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ultraviolet (uv)-reflective paint with ultraviolet germicidal irradiation (uvgi) improves decontamination of nosocomial bacteria on hospital room surfaces.an ultraviolet germicidal irradiation (uvgi) generator (the torch™, clordisys solutions, inc.) was used to compare the disinfection of surface coupons (plastic from a bedrail, stainless steel, and chrome-plated light switch cover) in a hospital room with walls coated with ultraviolet (uv)-reflective paint (lumacept) or standard paint. each surface coupon was inoculated with methicillin-resistant staphylococcus aureus (mrsa) or vancomycin-resistant enterococcus faecalis (vre), placed at 6 differe ...201728278065
effect of photodynamic therapy in combination with various irrigation protocols on an endodontic multispecies biofilm ex vivo.to analyse the antibacterial effect of photodynamic therapy (pdt) in combination with various irrigation protocols on a multispecies biofilm in root canals ex vivo.201728276583
identification of the cfr methyltransferase gene in enterococcus faecalis isolated from swine: first report in brazil. 201728274847
effectiveness of persea major kopp (lauraceae) extract against enterococcus faecalis: a preliminary in vitro study.persea major kopp (lauraceae) is a plant with wound healing, antibacterial, and analgesic properties. the aim of this study was to assess the in vitro antibacterial activity of the concentrated crude extract (cce) and ethyl acetate fraction (eaf) of this plant against enterococcus faecalis and compare it with calcium hydroxide [ca(oh)2] paste and 2% chlorhexidine digluconate (chx).201728264708
susceptibility to disinfectants in antimicrobial-resistant and -susceptible isolates of escherichia coli, enterococcus faecalis and enterococcus faecium from poultry - esbl / ampc-phenotype of escherichia coli is not associated with resistance to a quaternary ammonium compound, ddac.the spread of bacteria that are simultaneously resistant to disinfectants and antimicrobials would constitute an unsettling scenario. in order to explore an association between antimicrobial resistance and reduced susceptibility to biocides / microbicides like disinfectants in agriculture, we investigated escherichia (e.) coli (n = 438) and enterococci (n = 120) isolated from six different flocks of the same poultry farm with known history of antimicrobial treatment.201728261951
antibacterial efficacy of octenisept, alexidine, chlorhexidine, and sodium hypochlorite against enterococcus faecalis biofilms.the purpose of this study was to evaluate the antibacterial effectiveness of octenisept (oct; schülke & mayr gmbh, norderstedt, germany), 1% alexidine (alx) (santa cruz biotechnology, inc, santa cruz, ca), and 2% chlorhexidine (chx) against enterococcus faecalis biofilm using confocal laser scanning microscopy.201728258812
beneficial and safety properties of a corynebacterium vitaeruminis strain isolated from the cow rumen.corynebacterium vitaeruminis mru4 was isolated from the cow rumen and was differentiated from other isolates by rep-pcr and rapd and identified by 16s rrna sequencing. this strain presented higher survival rates for low ph and bile salts treatments, and it was able to survive and multiply in simulated gastric and intestinal environments. c. vitaeruminis mru4 had a 53.2% auto-aggregation rate, 42.4% co-aggregation rate with listeria monocytogenes scott a, 41.6% co-aggregation rate with enterococc ...201728258546
evaluation of 4% sodium hypochlorite in eliminating enterococcus faecalis from the root canal when used with three irrigation methods: an in vitro study.endodontic treatment removes all pathogens, such as enterococcus faecalis from pulp and root canals. the aim of this study is to assess the usefulness of sodium hypo-chlorite (naocl) in removing e. faecalis from the root canal used with three different irrigation methods.201728258267
antimicrobial efficacy of mixtures of nanosilver and zinc oxide eugenol against enterococcus faecalis.this study aimed to assess the antimicrobial efficacy of 0, 0.5, 2, and 5 wt% nanosilver in conjunction with zinc oxide eugenol (zoe) against enterococcus faecalis.201728258260
genetic diversity of enterococcus faecalis isolated from environmental, animal and clinical sources in malaysia.enterococcus faecalis ranks as one of the leading causes of nosocomial infections. a strong epidemiological link has been reported between e. faecalis inhabiting animals and environmental sources. this study investigates the genetic diversity, antibiotic resistance and virulence determinants in e. faecalis from three sources in malaysia. a total of 250 e. faecalis isolates were obtained consisting of 120 isolates from farm animals, 100 isolates from water sources and 30 isolates from hospitalize ...201728254461
[effects of epigallocatechin-3-gallate modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin].objective: to evaluate the effects of epigallocatechin-3-gallate (egcg) modification on the bonding stability of an etch-and-rinse adhesive to intraradicular dentin, and to find a new strategy to improve the stability of bonding interface. methods: egcg was incorporated into single bond 2 (sb2) with the concentration of 200 mg/l and 400 mg/l respectively to fabricated experimental adhesives group a and group b, while single bond 2 without egcg was used as control group. laser scanning confocal m ...201728253588
discovery of substituted oxadiazoles as a novel scaffold for dna gyrase inhibitors.dna gyrase and topoisomerase iv are type iia topoisomerases that are essential bacterial enzymes required to oversee the topological state of dna during transcription and replication processes. their atpase domains, gyrb and pare, respectively, are recognized as viable targets for small molecule inhibitors, however, no synthetic or natural product gyrb/pare inhibitors have so far reached the clinic for use as novel antibacterial agents, except for novobiocin which was withdrawn from the market. ...201728246042
confocal laser scanning, scanning electron, and transmission electron microscopy investigation of enterococcus faecalis biofilm degradation using passive and active sodium hypochlorite irrigation within a simulated root canal model.root canal irrigation is an important adjunct to control microbial infection. the aim of this study was to investigate the effect of 2.5% (wt/vol) sodium hypochlorite (naocl) agitation on the removal, killing, and degradation of enterococcus faecalis biofilm. a total of 45 root canal models were manufactured using 3d printing with each model comprising an 18 mm length simulated root canal of apical size 30 and taper 0.06. e. faecalis biofilms were grown on the apical 3 mm of the models for 10 da ...201728244230
enterococcus faecalis uses a pts permease and a host colonization-related abc transporter for maltodextrin uptake.maltodextrin is a mixture of maltooligosaccharides, which are produced by the degradation of starch or glycogen. they are mostly composed of α-1,4- and some α-1,6- linked glucose residues. genes presumed to code for the enterococcus faecalis maltodextrin transporter were induced during enterococcal infection. we therefore carried out a detailed study of maltodextrin transport in this organism. depending on their length (3 to 7 glucose residues), e. faecalis takes up maltodextrins either via malt ...201728242718
adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing.the sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. in order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast starmerella bombicola, which yielded both acidic c 18-1 ac ...201728242666
loop-mediated isothermal amplification label-based gold nanoparticles lateral flow biosensor for detection of enterococcus faecalis and staphylococcus aureus.the report describes a simple, rapid and sensitive assay for visual and multiplex detection of enterococcus faecalis and staphylococcus aureus based on multiple loop-mediated isothermal amplification (mlamp) and lateral flow biosensor (lfb). detection and differentiation of the ef0027 gene (e. faecalis-specific gene) and nuc gene (s. aureus-specific gene) were determined using fluorescein (fitc)-and digoxin-modified primers in the mlamp process. in the presence of biotin- and fitc-/digoxin-modif ...201728239371
genetic diversity and antibiotic resistance of enterococcus faecalis isolates from traditional korean fermented soybean foods.eighty-five enterococcus faecalis isolates collected from animals (40 isolates), meju (korean fermented soybean product; 27 isolates), humans (10 isolates), and various environmental samples (eight isolates) were subjected to multilocus sequence typing (mlst) to identify genetic differences between samples of different origins. mlst analysis resulted in 44 sequence types (sts), and the eburst algorithm clustered the sts into 21 clonal complexes (ccs) and 17 singletons. the predominant sts, st695 ...201728237994
efficacy of rifampicin combination therapy for the treatment of enterococcal infections assessed in vivo using a galleria mellonella infection model.enterococci are a leading cause of healthcare-associated infection worldwide and display increasing levels of resistance to many of the commonly used antimicrobials, making treatment of their infections challenging. combinations of antibiotics are occasionally employed to treat serious infections, allowing for the possibility of synergistic killing. the aim of this study was to evaluate the effects of different antibacterial combinations against enterococcal isolates using an in vitro approach a ...201728235571
antimicrobial resistance and virulence profile of enterococci isolated from poultry and cattle sources in nigeria.this study investigated the occurrence, antimicrobial resistance and virulence of enterococcus from poultry and cattle farms. three hundred and ninety samples: cloacal/rectal swabs (n = 260) and manure (n = 130] were processed for recovery of enterococcus species. standard bacteriological methods were used to isolate, identify and characterize enterococcus species for antimicrobial susceptibility and expression of virulence traits. detection of antibiotic resistance and virulence genes was carri ...201727987112
development of a rapid identification method for the differentiation of enterococcus species using a species-specific multiplex pcr based on comparative genomics.enterococci are lactic acid bacteria that are commonly found in food and in animal gut. since 16 s ribosomal rna (rrna) sequences, genetic markers for bacterial identification, are similar among several enterococcus species, it is very difficult to determine the correct species based on only 16 s rrna sequences. therefore, we developed a rapid method for the identification of different enterococcus species using comparative genomics. we compared 38 genomes of 13 enterococcus species retrieved fr ...201728229213
antibacterial efficacy of several intracanal medicaments for endodontic therapy.the antibacterial efficacy of intracanal medicaments for 2 and 7 days, in open apex root canals contaminated with enterococcus faecalis biofilms was compared. one hundred and thirty-eight standardized uniradicular human roots were inoculated with e. faecalis. colony forming units (cfu) were recorded before and after medication. samples were divided in two (2/7 days), and subdivided (n=8/group): triple antibiotic paste (tap); double antibiotic paste (dap); dental base paste (dbp), consisting of c ...201728228624
genotypic characterization of vancomycin-resistant enterococcus spp. in tertiary center, iran.enterococci infection rate, mortality and morbidity has been increased in recent decades accompanied by progressive emerging antimicrobial resistance. vancomycin-resistant enterococci (vre), important pathogen in hospitalized patients, have distinct antibiotic susceptibility to glycopeptides that varied genetically and can influence in choosing therapeutic agents. in this study, we aimed to determine the patterns of antimicrobial resistance according to the genotypic variations in vres.201728228089
requirement of the crors two-component system for resistance to cell-wall-targeting antimicrobials in enterococcus faecium.enterococci are serious opportunistic pathogens that are resistant to many cell-wall-targeting antibiotics. the crors two-component signaling system responds to antibiotic-mediated cell-wall stress and is critical for resistance to cell-wall-targeting antibiotics in enterococcus faecalis here, we identify and characterize an orthologous two-component system found in enterococcus faecium that is functionally equivalent to the crors system of e. faecalis deletion of crors in e. faecium resulted in ...201728223383
comparison of methods for identifying causative bacterial microorganisms in presumed acute endophthalmitis: conventional culture, blood culture, and pcr.identification of bacterial pathogens in endophthalmitis is important to inform antibiotic selection and treatment decisions. hemoculture bottles and polymerase chain reaction (pcr) analysis have been proposed to offer good detection sensitivity. this study compared the sensitivity and accuracy of a blood culture system, a pcr approach, and conventional culture methods for identification of causative bacteria in cases of acute endophthalmitis.201728222703
technological properties and biogenic amines production by bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk.technological properties and biogenic amine production were analyzed in 56 bacteriocinogenic lactococci and enterococci strains isolated from raw goat's milk. fifteen lactococci strains were able to reduce milk ph to 5.3 or lower after 6 h, while enterococci strains were initially slow in producing acids. lactococcus lactis subsp. lactis glc06 and three strains of enterococcus faecalis (gen20, gen22, and gen23) presented high proteolytic activity. l. lactis subsp. lactis glc06 and e. faecalis ge ...201728221886
conjugative type iv secretion in gram-positive pathogens: trag, a lytic transglycosylase and endopeptidase, interacts with translocation channel protein tram.conjugative transfer plays a major role in the transmission of antibiotic resistance in bacteria. pip501 is a gram-positive conjugative model plasmid with the broadest transfer host-range known so far and is frequently found in enterococcus faecalis and enterococcus faecium clinical isolates. the pip501 type iv secretion system is encoded by 15 transfer genes. in this work, we focus on the virb1-like protein trag, a modular peptidoglycan metabolizing enzyme, and the virb8-homolog tram, a potenti ...201728219792
enterocin as-48 as evidence for the use of bacteriocins as new leishmanicidal agents.we report the feasibility of enterocin as-48, a circular cationic peptide produced by enterococcus faecalis, as a new leishmanicidal agent. as-48 is lethal to leishmania promastigotes as well as to axenic and intracellular amastigotes at low micromolar concentrations, with scarce cytotoxicity to macrophages. as-48 induced a fast bioenergetic collapse of l. donovani promastigotes but only a partial permeation of their plasma membrane with limited entrance of vital dyes, even at concentrations bey ...201728167557
combination of ceftriaxone and ampicillin for the treatment of enterococcal endocarditis.the aim of this systematic review is to review all human trials assessing the efficacy and safety of ampicillin and ceftriaxone for enterococcal endocarditis and to discuss the clinical implications of the findings.201728166656
nipple shields in transaxillary breast augmentation.infection after breast augmentation occurs in 1.1% to 2.5% of patients. bacterial contamination of the implants could explain some complications of breast implant surgery, including infection, capsular contracture and even anaplastic large cell lymphoma. because of the evidence of bacterial spread from the nipple, nipple shields have been proposed as a routine maneuver to avoid contamination of the implants.201728166134
microbial shifts in the porcine distal gut in response to diets supplemented with enterococcus faecalis as alternatives to antibiotics.gut microbiota plays an important role in host health and nutrient digestion of animals. probiotics have become one of effective alternatives to antibiotics enhancing animal health and performance through modulating gut microbiota. previously, our research demonstrated that dietary enterococcus faecalis uc-100 substituting antibiotics enhanced growth and health of weaned pigs. to investigate the alterations of microbiota in the distal gut of pigs fed e. faecalis uc-100 substituting antibiotics, ...201728165001
screening for antibacterial and antibiofilm activities in astragalus angulosus.in a search for finding novel therapeutic agents, extracts from an endemic lebanese plant, astragalus angulosus, were evaluated for their potential in-vitro antibacterial and antibiofilm activities against three gram-positive bacterial strains; staphylococcus epidermidis (cip444), staphylococcus aureus (atcc25923), and enterococcus faecalis (atcc29212); in addition to two gram-negative strains, escherichia coli (atcc35218) and pseudomonas aeruginosa (atcc27853).201728163960
cold atmospheric pressure plasma elimination of clinically important single- and mixed-species biofilms.mixed-species biofilms reflect the natural environment of many pathogens in clinical settings and are highly resistant to disinfection methods. an indirect cold atmospheric-pressure air-plasma system was evaluated under two different discharge conditions for its ability to kill representative gram-positive (staphylococcus aureus) and gram-negative (pseudomonas aeruginosa) pathogens. plasma treatment of individual 24-h-old biofilms and mixed-species biofilms that contained additional species (ent ...201728161488
in vitro activity of oritavancin against gram-positive pathogens isolated in canadian hospital laboratories from 2011 to 2015.gram-positive bacterial pathogens isolated from patient specimens submitted to 15 canadian hospital laboratories from 2011 to 2015 were tested in the coordinating laboratory for susceptibility to oritavancin and comparative antimicrobial agents using the clinical and laboratory standards institute m07-a10 (2015) broth microdilution method. oritavancin's in vitro activity was equivalent to, or more potent than, vancomycin, daptomycin, linezolid, and tigecycline against methicillin-susceptible sta ...201728159446
synthesis, surfactant properties and antimicrobial activities of methyl glycopyranoside ethers.a series of amphiphilic methyl glucopyranoside ethers incorporating various alkyl chain lengths has been synthesized from commercially available methyl glucopyranosides following an acetalisation/hydrogenolysis sequence. the amphiphilic properties of ethers and acetal intermediates were evaluated. both families exhibit excellent surfactant properties with a maximum efficiency obtained for compounds bearing a linear dodecyl chain (cmc = 0.012 mm, γsat. = 30 mn m(-1)). antimicrobial activity studi ...201728157594
synthesis of novel pyrazinamide derivatives based on 3-chloropyrazine-2-carboxamide and their antimicrobial evaluation.aminodehalogenation of 3-chloropyrazine-2-carboxamide with variously substituted benzylamines yielded a series of fifteen 3-benzylaminopyrazine-2-carboxamides. four compounds possessed in vitro whole cell activity against mycobacterium tuberculosis h37rv that was at least equivalent to that of the standard pyrazinamide. mic values ranged from 6 to 42 μm. the best mic (6 μm) was displayed by 3-[(4-methylbenzyl)amino]pyrazine-2-carboxamide (8) that also showed low cytotoxicity in the hepg2 cell li ...201728157178
readmission rate and causes at 90-day after radical cystectomy in patients on early recovery after surgery protocol.background: radical cystectomy (rc) is associated with high risk of early and late perioperative complications, and readmissions. the enhanced recovery after surgery (eras) protocol has been applied to rc showing decreased hospital stay without increased morbidity. objective: to evaluate the specific causes of hospital readmissions in rc patients treated before and after adoption of an eras protocol at our institution. methods: we retrospectively evaluated the outcome of 207 rc patients on eras ...201728149935
sealing efficacy of mineral trioxide aggregate with and without nanosilver for root end filling: an in vitro bacterial leakage study.various materials have been added to mineral trioxide aggregate to enhance its properties. this study was aimed to compare the sealing efficacy of mta with and without nanosilver using bacterial leakage approach.201728149459
retrospective comparative analysis of cardiovascular implantable electronic device infections with and without the use of antibacterial envelopes.cardiovascular implantable electronic device (cied) infections are associated with morbidity and mortality. peri-operative systemic intravenous antibiotic prophylaxis reduces the rate of cied infections. aigisrx, a polymer envelope implanted with the cied, releases minocycline and rifampin, and has been introduced to reduce infections.201728131641
diversity of enterococcus faecalis genotypes from multiple oral sites associated with endodontic failure using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction.the aim of this study was to evaluate the diversity and similarity of enterococcus faecalis genotype isolates from multiple oral sites using repetitive sequence-based polymerase chain reaction and arbitrarily primed polymerase chain reaction (ap-pcr).201728131414
[update on antibiotic resistance in gram-positive bacteria].antimicrobial resistance among gram-positive bacteria, especially in staphylococcus aureus, enterococcus faecium, enterococcus faecalis, and streptococcus pneumoniae, is a serious threat to public health. these microorganisms have multiple resistance mechanisms to agents currently used in clinical practice. many of these resistance mechanisms are common to all 4 of these bacterial species, but other mechanisms seem to be more specific. the prevalence and dissemination of these mechanisms varies ...201728129816
antimicrobial activity of endodontic medicaments and vehicles using agar well diffusion method on facultative and obligate anaerobes.the aim of this study was to determine the relative antimicrobial effectiveness of these endodontic medicaments and various vehicles using an agar well diffusion assay.201728127166
a comparison of antibacterial and antibiofilm efficacy of phenothiazinium dyes between gram positive and gram negative bacterial biofilm.antimicrobial photodynamic therapy (apdt) is a process that generates reactive oxygen species (ros) in presence of photosensitizer, visible light and oxygen which destroys the bacterial cells. we investigated the photoinactivation efficiency of phenothiazinium dyes and the effect of ros generation on gram positive and gram negative bacterial cell as well as on biofilm.201728119141
growth, biogenic amine production and tyrdc transcription of enterococcus faecalis in synthetic medium containing defined amino acid concentrations.the tyraminogenic potential of the strains enterococcus faecalis ef37 and atcc 29212 was investigated in a synthetic medium containing defined amounts of tyrosine and phenylalanine at different temperatures.201728117533
catheter-associated urinary tract infection (cauti) after term cesarean delivery: incidence and risk factors at a multi-center academic institution.the purpose of this study is to identify the rate of catheter-associated urinary tract infection (cauti) after cesarean delivery (cd) and to determine if any factors increase risk of infection.201728114875
comparative efficacies of daptomycin, vancomycin, and linezolid in experimental enterococcal peritonitis.enterococci have become increasingly important pathogens for nosocomial infection (e.g. bacteremia, intra-abdominal infection, endocarditis, etc.), related to their intrinsic resistance to many antibiotics. although the in vitro susceptibility of daptomycin (dap) against enterococci is well established, the food and drug administration has only approved its use for complicated skin and skin structure infections induced by enterococcus faecalis. in this study we evaluated the potential therapeuti ...201728108098
antibacterial activity, computational analysis and host toxicity study of thymol-sulfonamide conjugates.methicillin-resistant staphylococcus aureus (mrsa) and vancomycin resistant enterococcus faecalis (vre) are notorious pathogenic multidrug resistant (mdr) bacteria in both hospital and community sectors, and today the first antibacterial drug sulfamethoxazole is ineffective. the monoterpene phenol, thymol was conjugated with seven sulfa drug derivatives individually, adopting the dye-azo synthesis protocol, and conjugates were characterized using spectral analysis techniques such as, uv, ftir, m ...201728107695
draft genome sequence of an enterococcus faecalis atcc 19433 siphovirus isolated from raw domestic sewage.we previously isolated and characterized an enterococcus faecalis atcc 19433 siphovirus from raw domestic sewage as a viral indicator of human fecal pollution. here, we report the draft genome sequence of this bacteriophage.201728104647
model systems for the study of enterococcal colonization and infection.enterococcus faecalis and enterococcus faecium are common inhabitants of the human gastrointestinal tract, as well as frequent opportunistic pathogens. enterococci cause a range of infections including, most frequently, infections of the urinary tract, catheterized urinary tract, bloodstream, wounds and surgical sites, and heart valves in endocarditis. enterococcal infections are often biofilm-associated, polymicrobial in nature, and resistant to antibiotics of last resort. understanding enteroc ...201728102784
interspecies transfer of vancomycin, erythromycin and tetracycline resistance among enterococcus species recovered from agrarian sources.enterococci are now well recognised for their ability to transfer antibiotic resistance and for their association with nosocomial infections, but less is known regarding their relevance in the wider environment. enterococcus faecalis and enterococcus faecium were isolated from a range of agrarian associated sources (low-flow water, septic tank, poultry litter, high flow water, slurry/soil) and were assessed for latent ability to transfer antimicrobial resistance.201728100194
multidrug-resistant enterococcal infections: new compounds, novel antimicrobial therapies?over the past two decades infections due to antibiotic-resistant bacteria have escalated world-wide, affecting patient morbidity, mortality, and health care costs. among these bacteria, enterococcus faecium and enterococcus faecalis represent opportunistic nosocomial pathogens that cause difficult-to-treat infections because of intrinsic and acquired resistance to a plethora of antibiotics. in recent years, a number of novel antimicrobial compound classes have been discovered and developed that ...201728209400
early in vitro development of daptomycin non-susceptibility in high-level aminoglycoside-resistant enterococcus faecalis predicts the efficacy of the combination of high-dose daptomycin plus ampicillin in an in vivo model of experimental endocarditis. 201728204495
some technological properties of lactic acid bacteria isolated from dahi and datshi, naturally fermented milk products of bhutan.dahi and datshi are common naturally fermented milk (nfm) products of bhutan. population of lactic acid bacteria (lab) in dahi (ph 3.7) and datshi (ph 5.2) was 1.4 × 10(7) and 3.9 × 10(8) cfu/ml, respectively. based on 16s rrna gene sequencing isolates of lab from dahi and datshi were identified as enterococcus faecalis, e. faecium, lactococcus lactis subsp. lactis. lab strains were tested for some technological properties. all lab strains except e. faecalis ch2:17 caused coagulation of milk at ...201728203227
diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on listeria monocytogenes and salmonella enterica serovar enteritidis in a co-culture model.food-borne infections cause huge economic and human life losses. listeria monocytogenes and salmonella enterica serovar enteritidis are among the top ranking pathogens causing such losses. control of such infections is hampered by persistent contamination of foods and food-processing environments, resistance of pathogens to sanitizing agents, existence of heterogeneous populations of pathogens (including culturable and viable but non-culturable cells) within the same food items, and inability to ...201728202007
effects of lactobacillus casei and enterococcus faecalis on growth performance, immune function and gut microbiota of suckling piglets.this study was carried out to investigate the effects of orally administrated lactobacillus casei and enterococcus faecalis on performance, immune function and gut microbiota of suckling piglets. neonatal piglets (n = 120) were randomly assigned to 4 groups, with 30 suckling piglets in each group. the piglets were from 15 litters, one male and one female piglet were selected for each group in each litter. the control group was administrated with normal saline, the other groups with l. casei or e ...201728201936
linezolid-resistant enterococci in polish hospitals: species, clonality and determinants of linezolid resistance.the significant increase of the linezolid-resistant enterococci (lre) has been observed in polish hospitals since 2012 and our study aimed at elucidating the possible reasons for this phenomenon. polish lre isolates were analysed by multilocus-sequence typing (mlst) and multiple locus variable-number tandem repeat (vntr) analysis (mlva), polymerase chain reaction (pcr) and pcr-restriction fragment length polymorphism (pcr-rflp) to establish clonal relatedness and mechanism of linezolid resistanc ...201728197728
antibacterial effect of urushiol on e. faecalis as a root canal irrigant.the purpose of this study was to compare the antibacterial activity of urushiol against enterococcus faecalis (e. faecalis) to that of naocl.201728194365
is there a place for corticosteroids in the therapy of infective endocarditis? report of a case and review. 201728189738
synthesis and antimicrobial evaluation of cationic low molecular weight amphipathic 1,2,3-triazoles.a library of 28 small cationic 1,4-substituted 1,2,3-triazoles was prepared for studies of antimicrobial activity. the structures addressed the pharmacophore model of small antimicrobial peptides and an amphipathic motif found in marine antimicrobials. eight compounds showed promising antimicrobial activity, of which the most potent compound 10b displayed minimum inhibitory concentrations of 4-8μg/ml against streptococcus agalacticae, staphylococcus aureus, pseudomonas aeruginosa, escherichia co ...201728189422
evaluation of antimicrobial photodynamic therapy using indocyanine green and near-infrared diode laser against enterococcus faecalis in infected human root canals.photodynamic therapy (pdt) is considered a promising adjunct to the currently available endodontic disinfection techniques leading to more effective reduction of intracanal bacteria. the present ex vivo study aimed to assess the antimicrobial effect of pdt using indocyanine green (icg) as photosensitizer and a near-infrared (nir) diode laser in root canals of human teeth infected with enterococcus faecalis.201728186464
effectiveness of repeated photodynamic therapy in the elimination of intracanal enterococcus faecalis biofilm: an in vitro study.the study aimed to investigate the effectiveness of photodynamic therapy in the elimination of intracanal enterococcus faecalis biofilm and to analyse how a repeated light irradiation, replenishment of oxygen and photosensitiser affect the results of the photodynamic disinfecting protocol. after chemomechanical preparation, 46 single-rooted human teeth were infected with a clinical strain of e. faecalis and incubated for a week in microaerobic conditions. the experimental procedures included gro ...201728185089
correlation between infective factors and antibiotic resistance in enterococci clinical isolates in west of iran.the present study was done to scrutinize the possible relation between infective genes and antimicrobial resistance in enterococcus faecalis and enterococcus faecium. considering the fact that the presence of recognized infective determinants among clinical isolates may promote the emergence of infections and persistence of enterococci in hospital settings, which can lead to an increase in antimicrobial resistance. 175 e. faecalis and 67 e. faecium isolated from clinical specimens were used. the ...201728184339
characterization of enterococcus faecalis isolated from the cloaca of "fancy breeds" and confined chickens.the aim was to investigate the intestinal community of e. faecalis from healthy confined non-industrialized chicken.201728177186
genome sequence of enterococcus faecalis strain cg_e.enterococcus faecalis cg_e is a gram-positive, lactic acid-producing coccus. the draft genome of e. faecalis strain cg_e comprises 2,969,881 bp and exhibits a g+c content of 37.34%. the genome encodes 2,848 predicted protein-encoding and 97 rna genes.201728082508
[early results of adjuvant topical treatment of recurrent osteomyelitis with absorbable antibiotic carriers].background treatment of musculoskeletal infections principally consists of radical surgical debridement and systemic administration of antibiotics. additional local antibiotic therapy is not yet generally established, and lacks evidence-based proof of efficacy. nonetheless, there are a variety of practical approaches, as most specialised departments are unwilling to forego this option. the established polymethylmetacrylate (pmma) carrier system has a number of practical disadvantages. this has l ...201728081583
characterization of multi-drug resistant enterococcus faecalis isolated from cephalic recording chambers in research macaques (macaca spp.).nonhuman primates are commonly used for cognitive neuroscience research and often surgically implanted with cephalic recording chambers for electrophysiological recording. aerobic bacterial cultures from 25 macaques identified 72 bacterial isolates, including 15 enterococcus faecalis isolates. the e. faecalis isolates displayed multi-drug resistant phenotypes, with resistance to ciprofloxacin, enrofloxacin, trimethoprim-sulfamethoxazole, tetracycline, chloramphenicol, bacitracin, and erythromyci ...201728081148
ozone therapy as an adjuvant for endondontic protocols: microbiological - ex vivo study and citotoxicity analyses.this study evaluated the antimicrobial efficacy of ozone therapy in teeth contaminated with pseudomonas aeruginosa, enterococcus faecalis, and staphylococcus aureus using a mono-species biofilm model. parallel to this, the study aimed to evaluate the cytotoxicity of ozone for human gingival fibroblasts. material and methods: one hundred and eighty single-root teeth were contaminated with a mono-species biofilm of enterococcus faecalis, pseudomonas aeruginosa, and staphylococcus aureus. groups we ...201728076466
effect of ultrasonic streaming on intra-dentinal disinfection and penetration of calcium hydroxide paste in endodontic treatment.the antimicrobial effect of ultrasonic agitation of calcium hydroxide (ch) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (clsm) and microbiological culture.201728076462
a novel phosphodiesterase of the gdpp family modulates cyclic di-amp levels in response to cell membrane stress in daptomycin-resistant enterococci.substitutions in the liafsr membrane stress pathway are frequently associated with the emergence of antimicrobial peptide resistance in both enterococcus faecalis and enterococcus faecium cyclic di-amp (c-di-amp) is an important signal molecule that affects many aspects of bacterial physiology, including stress responses. we have previously identified a mutation in a gene (designated yybt) in e. faecalis that was associated with the development of daptomycin resistance, resulting in a change at ...201728069645
probiotic e. coli nissle 1917 biofilms on silicone substrates for bacterial interference against pathogen colonization.bacterial interference is an alternative strategy to fight against device-associated bacterial infections. pursuing this strategy, a non-pathogenic bacterial biofilm is used as a live, protective barrier to fence off pathogen colonization. in this work, biofilms formed by probiotic escherichia coli strain nissle 1917 (ecn) are investigated for their potential for long-term bacterial interference against infections associated with silicone-based urinary catheters and indwelling catheters used in ...201728069496
design, synthesis and antibacterial evaluation of novel pleuromutilin derivatives possessing piperazine linker.a series of pleuromutilin derivatives bearing piperazine ring have been reported. the in vitro antibacterial activities of the synthetic derivatives against mrsa (atcc 43300), staphylococcus aureus (atcc 29213), enterococcus faecalis (atcc 29212), enterococcus faecium (atcc35667) and escherichia coli (atcc25922) were evaluated by the broth dilution method. most of the synthesized derivatives displayed potent activities. compounds 11c, 12a and 12c were found to be the most active antibacterial de ...201728068600
the effect of diode laser on planktonic enterococcus faecalis in infected root canals in an ex vivo model.this study examined the bactericidal effect of diode laser irradiation against intracanal enterococcus faecalis.201728068207
bactericidal efficacy of photodynamic therapy and chitosan in root canals experimentally infected with enterococcus faecalis: an in vitro study.to evaluate the antibacterial efficacy of photodynamic therapy (pdt) and chitosan against enterococcus faecalis and assess the possible enhancive effect of chitosan on the photosensitizer methylene blue in experimentally infected root canals of extracted human teeth in vitro.201728068186
genetic relatedness of faecal coliforms and enterococci bacteria isolated from water and sediments of the apies river, gauteng, south africa.to date, the microbiological quality of river sediments and its impact on water resources are not included in the water quality monitoring assessment. therefore, the aim of this study was to establish genetic relatedness between faecal coliforms and enterococci isolated from the river water and riverbed sediments of apies river to better understand the genetic similarity of microorganisms between the sediment and water phases. indicator bacteria were subjected to a molecular study, which consist ...201728063147
fosfomycin: mechanism and resistance.fosfomycin, a natural product antibiotic, has been in use for >20 years in spain, germany, france, japan, brazil, and south africa for urinary tract infections (utis) and other indications and was registered in the united states for the oral treatment of uncomplicated utis because of enterococcus faecalis and escherichia coli in 1996. it has a broad spectrum, is bactericidal, has very low toxicity, and acts as a time-dependent inhibitor of the mura enzyme, which catalyzes the first committed ste ...201728062557
critical role of tedizolid in the treatment of acute bacterial skin and skin structure infections.tedizolid phosphate has high activity against the gram-positive microorganisms mainly involved in acute bacterial skin and skin structure infections, such as strains of staphylococcus aureus (including methicillin-resistant s. aureus strains and methicillin-sensitive s. aureus strains), streptococcus pyogenes, streptococcus agalactiae, the streptococcus anginosus group, and enterococcus faecalis, including those with some mechanism of resistance limiting the use of linezolid. the area under the ...201728053508
isolation of rhamnolipids-producing cultures from faeces: influence of interspecies communication on the yield of rhamnolipid congeners.the aim of this study was to evaluate the ability of bacterial cultures isolated from cattle, poultry or pig faeces and manure to produce rhamnolipids, as well as to investigate the influence of interspecies communication on possible quantitative differences in the production of rhamnolipid congeners. initial screening methods (oil spreading, drop collapse, haemolytic activity and emulsification activity) showed that approximately 36% of the 51 isolated cultures exhibited the ability to produce ...201728043869
quantification of the d-ala-d-lac-terminated peptidoglycan structure in vancomycin-resistant enterococcus faecalis using a combined solid-state nuclear magnetic resonance and mass spectrometry analysis.induction of vancomycin resistance in vancomycin-resistant enterococci (vre) involves replacement of the d-ala-d-ala terminus of peptidoglycan (pg) stems with d-ala-d-lac, dramatically reducing the binding affinity of vancomycin for lipid ii. effects from vancomycin resistance induction in enterococcus faecalis (atcc 51299) were characterized using a combined solid-state nuclear magnetic resonance (nmr) and liquid chromatography-mass spectrometry (lc-ms) analysis. solid-state nmr directly measur ...201728040891
a comparison between effect of photodynamic therapy by led and calcium hydroxide therapy for root canal disinfection against enterococcus faecalis: a randomized controlled trial.insufficient root canal disinfection is one of the main reasons for persistent periapical pathology. photodynamic therapy (pdt) has been proven effective in disinfecting infected root canals. the aim of this study was to evaluate the antimicrobial effect of photo activated disinfection (pad) when using toluidine blue as photosensitizer and a led lamp after the conventional treatment, and comparing it with calcium hydroxide therapy in vivo.201728040502
novel lnu(g) gene conferring resistance to lincomycin by nucleotidylation, located on tn6260 from enterococcus faecalis e531.to identify a novel putative lincosamide resistance gene determinant in a swine enterococcus faecalis e531 exhibiting a lincosamide resistance/macrolide susceptibility (l r m s ) phenotype and to determine its location and genetic environment.201728039271
study on the excision and integration mediated by class 1 integron in enterococcus faecalis.recognized as a mobile genetic element, integron is correlated to the excision and integration of exogenous genes, especially bacterial resistance genes. however, most of the investigations focused on gram-positive bacteria with few exceptions. in this study, enterococcus faecalis was selected to investigate the excision and integration of class 1 integron. a total of eight plasmids were subjected to establish the transformants for excision and integration test. as results showed, positive excis ...201728390978
stimulatory effects of heat-killed enterococcus faecalis on cell-mediated immunity in fish.intracellular bacterial and viral diseases are widespread in the aquaculture industry and cause serious economic losses. development of effective vaccines and adjuvants that can induce cell-mediated immunity is urgently needed for prevention of these diseases. here we report the immunostimulatory effects of probiotic bacteria ''e. faecalis'' in ginbuna crucian carp carassius auratus langsdorfii. intraperitoneal injection of heat-killed e. faecalis induced an increase in cd4-1(+) lymphocytes, cd8 ...201728389237
antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens.adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ...201728377765
poly(methyl methacrylate) surface modification for surfactant-free real-time toxicity assay on droplet microfluidic platform.microfluidic droplet reactors have many potential uses, from analytical to synthesis. stable operation requires preferential wetting of the channel surface by the continuous phase which is often not fulfilled by materials commonly used for lab-on-chip devices. here we show that a silica nanoparticle (sinp) layer coated onto a poly(methyl methacrylate) (pmma) and other thermoplastics surface enhances its wetting properties by creating nanoroughness, and allows simple grafting of hydrocarbon chain ...201728375597
pharmacodynamics of ceftaroline plus ampicillin against enterococcus faecalis in an in vitro pharmacokinetic/pharmacodynamic model of simulated endocardial vegetations.the combination of ampicillin plus ceftaroline has been suggested to be more reliably synergistic against enterococcus faecalis than ampicillin plus ceftriaxone using time-kill methods. the purpose of this study was to determine if this trend persists in a two-compartment model of simulated endocardial vegetations (sev) using clinically relevant pharmacokinetic exposures of these antimicrobials. three clinically derived e. faecalis strains were included in the study. the mics of study antimicrob ...201728096164
in vitro and ex vivo microbial leakage assessment in endodontics: a literature review.the aim of this study was to perform a literature review of published in-vitro and ex-vivo studies, which evaluated microbial leakage in endodontics in the past 10 years. a comprehensive electronic literature search was carried out in pubmed database for english articles published from 2005 to 2016 using the keywords "endodontics," "in vitro," "ex vivo," "microbial leakage," "microbial penetration," "saliva," "enterococcus faecalis," "e. faecalis," "endodontic sealers," "temporary filling materi ...201728032041
fournier's gangrene during acth therapy.fournier's gangrene is an infectious necrotizing fasciitis of the perineal, genital, or perianal regions and is uncommon in children. adrenocorticotropic hormone (acth) is effective for the treatment of infantile spasms; however, suppression of immune function is one of the major adverse effects of this approach. we encountered a 2-month-old boy with infantile spasms that had been treated with acth and had developed complicating fournier's gangrene. strangulation of a right inguinal hernia was o ...201728007393
detection of linezolid resistance due to the optra gene in enterococcus faecalis from poultry meat from the american continent (colombia).three enterococcus isolates obtained from retail chicken collected in 2010-11 as part of the colombian integrated program for antimicrobial resistance surveillance (coipars) showed reduced susceptibility towards linezolid (mic 8 mg/l).201727999039
bactericidal effect of hydroxyl radicals generated by the sonolysis and photolysis of hydrogen peroxide for endodontic applications.the aim of endodontic root canal treatment is the elimination of bacteria and their products from an infected tooth root canal. to effectively disinfect a root canal, an ultrasonic irrigation system, in which hydroxyl radicals (ho(·)) generated artificially by sonolysis of h2o2, was developed previously for endodontic applications and was demonstrated to have bactericidal efficacy against enterococcus faecalis. to improve this system, we examined the in vitro bactericidal effects of ho(·) genera ...201727998731
entrococcus faecalis elimination in root canals using silver nanoparticles, photodynamic therapy, diode laser, or laser-activated nanoparticles: an in vitro study.the aim of this study was to compare the efficacy of silver nanoparticles (agnps), an 810-nm diode laser (dl), conventional photodynamic therapy (pdt) with the use of indocyanine green (icg) photosensitizer, and modified pdt with the use of agnps for the disinfection of root canals inoculated with enterococcus faecalis.201728027821
evaluation of enterococcus faecalis adhesion, penetration, and method to prevent the penetration of enterococcus faecalis into root cementum: confocal laser scanning microscope and scanning electron microscope analysis.to ascertain the role of enterococcus faecalis in persistent infection and a possible method to prevent the penetration of e. faecalis into root cementum.201727994316
ofloxacin loaded gellan/pva nanofibers - synthesis, characterization and evaluation of their gastroretentive/mucoadhesive drug delivery potential.the purpose of this investigation is to formulate a gastroretentive sustained drug release system for ofloxacin to improve its retention time, pharmacological activity, bioavailability and therapeutic efficacy in the stomach. ofloxacin loaded gellan/poly vinyl alcohol (pva) nanofibers were fabricated using a simple and versatile electrospinning technique. the fabricated nanofibers were evaluated for percent drug encapsulation efficiency and in vitro drug release in simulated gastric medium (ph1. ...201727987752
culture methods impact recovery of antibiotic-resistant enterococci including enterococcus cecorum from pre- and postharvest chicken.pathogenic strains of enterococcus cecorum (ec) expressing multidrug resistance have emerged. in national antimicrobial resistance monitoring system (narms) data, ec is rarely recovered from chickens. two narms methodologies (fda and usda) were compared with standard culture (sc) techniques for recovery of ec. narms methods failed to detect ec in 58 caecal samples, 20 chicken breast or six whole broiler samples. ec was recovered from 1 of 38 (2·6%) and 2 of 38 (5·2%) preharvest spinal lesions (u ...201727987523
integrated multivariate analysis with nondetects for the development of human sewage source-tracking tools using bacteriophages of enterococcus faecalis.we developed sewage-specific microbial source tracking (mst) tools using enterococci bacteriophages and evaluated their performance with univariate and multivariate analyses involving data below detection limits. newly isolated enterococci faecalis bacterial strains aim06 (dsm100702) and sr14 (dsm100701) demonstrated 100% specificity and 90% sensitivity to human sewage without detecting 68 animal manure pooled samples of cats, chickens, cows, dogs, ducks, pigs, and pigeons. aim06 and sr14 bacter ...201727983829
phage-inducible islands in the gram-positive cocci.the sapis are a cohesive subfamily of extremely common phage-inducible chromosomal islands (picis) that reside quiescently at specific att sites in the staphylococcal chromosome and are induced by helper phages to excise and replicate. they are usually packaged in small capsids composed of phage virion proteins, giving rise to very high transfer frequencies, which they enhance by interfering with helper phage reproduction. as the sapis represent a highly successful biological strategy, with many ...201727959343
effect of the association of nonsteroidal anti-inflammatory and antibiotic drugs on antibiofilm activity and ph of calcium hydroxide pastes.the objective of the present study was to evaluate the in vitro antibiofilm activity and ph of calcium hydroxide associated with different nonsteroidal anti-inflammatory drugs (nsaids).201727939738
presence and resistance of streptococcus agalactiae in vaginal specimens of pregnant and adult non-pregnant women and association with other aerobic bacteria.aim to determine the prevalence rate and resistance profile of streptococcus agalactiae (s. agalactiae) in vaginal swabs of pregnant and adult non-pregnant women in the tuzla region, bosnia and herzegovina (b&h), as well as its association with other aerobic bacteria. methods this prospective study included 200 women, 100 pregnant and 100 adult non-pregnant. the research was conducted at the institute of microbiology, university clinical center tuzla from october to december 2015. standard aerob ...201727917849
relationship between enterococcus faecalis infective endocarditis and colorectal neoplasm: preliminary results from a cohort of 154 patients.the association between streptococcus bovis group infective endocarditis and colorectal neoplasm (crn) is well-known. however, no studies have assessed the association between enterococcus faecalis infective endocarditis (efie) and crn. we aimed to determine whether the prevalence of crn is higher in patients with efie and an unclear source of infection than in patients with efie and a known source of infection or in the general population.201727916708
therapeutic effects of gold nanoparticles synthesized using musa paradisiaca peel extract against multiple antibiotic resistant enterococcus faecalis biofilms and human lung cancer cells (a549).botanical-mediated synthesis of nanomaterials is currently emerging as a cheap and eco-friendly nanotechnology, since it does not involve the use of toxic chemicals. in the present study, we focused on the synthesis of gold nanoparticles using the aqueous peel extract of musa paradisiaca (mppe-aunps) following a facile and cheap fabrication process. the green synthesized mppe-aunps were bio-physically characterized by uv-vis spectroscopy, ftir, xrd, tem, zeta potential analysis and edx. mppe-aun ...201727916691
antibody guided molecular imaging of infective endocarditis.in this protocol, we describe the application of using a high affinity monoclonal antibody generated against the major pilin protein component of the pilin structure of enterococcus faecalis as a pet imaging agent for enterococcal endocarditis detection. the anti-pilin -mab 64cu conjugate was able to specifically label enterococcal endocarditis vegetation in vivo in a rodent endocarditis model. by targeting pili, a covalently linked surface antigen extending from the bacterial surface, we provid ...201727914083
biosynthesis of selenium nanoparticles using enterococcus faecalis and evaluation of their antibacterial activities.microorganisms are capable of synthesizing metal nanoparticles, and specifically enterococcus faecalis bacteria were tested for its ability to synthesize selenium nanoparticles (se-nps) from sodium selenite. the biosynthesized se-nps were spherical in shape with the size range of 29-195nm. also, the tem microscopy showed the accumulation of nano-structures as extracellular deposits. the ability of the bacteria to tolerate high levels of toxic selenite was studied by changing with different conce ...201727908405
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