Publications

TitleAbstractYear(sorted descending)
Filter
PMID
Filter
rilyd4, a human cd59 inhibitor, enhances complement-dependent cytotoxicity of ofatumumab against rituximab-resistant b-cell lymphoma cells and chronic lymphocytic leukemia.ofatumumab is an anti-cd20 antibody recently approved for treatment of fludarabine and alemtuzumab refractory chronic lymphocytic leukemia (cll); it mediates much stronger complement-dependent cytotoxicity (cdc) than rituximab. human cd59, a key membrane complement regulator that inhibits cdc, is highly expressed in b-cell malignancies and its upregulation is an important determinant of the sensitivity of b-cell malignancies to rituximab treatment. previously, we have shown that the potent cd59 ...201121918174
A lingual abscess caused by Streptococcus intermedius.Lingual abscesses are rare. We describe a case in a healthy female with no recent history of trauma. The organism recovered by culture of drainage material collected prior to antibiotic treatment was Streptococcus intermedius, an organism recognized as flora of the oropharynx and associated with abscess formation. The isolate was resistant to clindamycin, which was the antibiotic therapy that the patient received.201122116986
role of streptococcus intermedius dnak chaperone system in stress tolerance and pathogenicity.streptococcus intermedius is a facultatively anaerobic, opportunistic pathogen that causes purulent infections and abscess formation. the dnak chaperone system has been characterized in several pathogenic bacteria and seems to have important functions in stress resistance and pathogenicity. however, the role of dnak in s. intermedius remains unclear. therefore, we constructed a dnak knockout mutant that exhibited slow growth, thermosensitivity, accumulation of groel in the cell, and reduced cyto ...201121822788
[brain abscess as the first clinical manifestation of isolated pulmonary arteriovenous malformation without rendu-osler disease].brain abscesses occur in 5 to 13 % of patients with pulmonary arteriovenous malformation (pavm), more often present in rendu-osler-weber disease or hereditary hemorrhagic telangiectasia (hht).201120466398
efficacy and safety of adjunctive local moxifloxacin delivery in the treatment of periodontitis.moxifloxacin exerts excellent antibacterial activity against most putative periodontal pathogens and has been shown to kill bacteria in biofilm and host cells.201120843236
clustering of subgingival microbial species in adolescents with periodontitis.it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ...201121410554
spiramycin resistance in human periodontitis microbiota.purpose: the occurrence of in vitro resistance to therapeutic concentrations of spiramycin, amoxicillin, and metronidazole was determined for putative periodontal pathogens isolated in the united states. materials and methods: subgingival plaque specimens from 37 consecutive adults with untreated severe periodontitis were anaerobically cultured, and isolated putative periodontal pathogens were identified to a species level. in vitro resistance to spiramycin at 4 μg/ml, amoxicillin at 8 μg/ml, an ...201121524712
human cd59 inhibitor sensitizes rituximab-resistant lymphoma cells to complement-mediated cytolysis.rituximab efficacy in cancer therapy depends in part on induction of complement-dependent cytotoxicity (cdc). human cd59 (hcd59) is a key complement regulatory protein that restricts the formation of the membrane attack complex, thereby inhibiting induction of cdc. hcd59 is highly expressed in b-cell non-hodgkin's lymphoma (nhl), and upregulation of hcd59 is an important determinant of the sensitivity of nhl cells to rituximab treatment. here, we report that the potent hcd59 inhibitor rilyd4 enh ...201121252115
application of a novel inhibitor of human cd59 for the enhancement of complement-dependent cytolysis on cancer cells.many monoclonal antibodies (mabs) have been extensively used in the clinic, such as rituximab to treat lymphoma. however, resistance and non-responsiveness to mab treatment have been challenging for this line of therapy. complement is one of the main mediators of antibody-based cancer therapy via the complement-dependent cytolysis (cdc) effect. cd59 plays a critical role in resistance to mabs through the cdc effect. in this paper, we attempted to investigate whether the novel cd59 inhibitor, rec ...201121258360
induction of experimental endocarditis by continuous low-grade bacteremia mimicking spontaneous bacteremia in humans.transient high-grade bacteremia following invasive procedures carries a risk of infective endocarditis (ie). this is supported by experimental endocarditis. on the other hand, case-control studies showed that ie could be caused by cumulative exposure to low-grade bacteremia occurring during daily activities. however, no experimental demonstration of this latter possibility exists. this study investigated the infectivity in animals of continuous low-grade bacteremia compared to that of brief high ...201121321073
mapping the intermedilysin-human cd59 receptor interface reveals a deep correspondence with the binding site on cd59 for complement binding proteins c8{alpha} and c9.cd59 is a glycosylphosphatidylinositol-anchored protein that inhibits the assembly of the terminal complement membrane attack complex (mac) pore, whereas streptococcus intermedius intermedilysin (ily), a pore forming cholesterol-dependent cytolysin (cdc), specifically binds to human cd59 (hcd59) to initiate the formation of its pore. the identification of the residues of ily and hcd59 that form their binding interface revealed a remarkably deep correspondence between the hcd59 binding site for i ...201121507937
multiple lung abscesses secondary to a uterine empyema caused by an intrauterine device. 201121533605
encephalitozoon cuniculi genotype i as a causative agent of brain abscess in an immunocompetent patient.a brain abscess caused by encephalitozoon cuniculi genotype i together with streptococcus intermedius occurred in a patient without major immunocompromise and with diabetes. distinct clinical signs were hemiparesis and epilepsy. the microsporidium was observed in the abscess aspirate, and its specific dna was detected also in stool and urine. the patient was successfully treated with albendazole and mebendazole.201121593268
validation of the z++rich burn-biofilm model.background: despite advances in the use of topical and parenteral antimicrobial therapy and the practice of early tangential burn-wound excision, bacterial infection remains a major problem in the management of burn victims today. the purpose of this study was to design and evaluate a polyspecies biofilm model with bacteria known to cause severe infections in burn patients. the model is simple to prepare, maintain and analyse, and allows for short-term exposure to antimicrobials. results: initia ...201121724333
[a brain abscess as a complication of hepatopulmonary syndrome coexisting with interstitial pneumonia].a 76-year-old woman with a 20-year history of chronic hepatitis c was referred to our hospital for worsening exertional dyspnea. she had been given a diagnosis of interstitial pneumonia based on chest computed tomography findings 5 years previously. contrast-enhanced echocardiography confirmed an intrapulmonary right-to-left shunt, and therefore we diagnosed hepatopulmonary syndrome comorbid with interstitial pneumonia. in july 2009 she was admitted to our hospital with a low grade fever, headac ...201121842692
tubo-ovarian abscess during therapy of chronic hepatitis c with pegylated interferon and ribavirin.serious infections are rare complications of standard treatment in chronic hepatitis c with pegylated interferon alpha (peg ifn) and ribavirin.201121407153
splenic abscess and multiple brain abscesses caused by streptococcus intermedius in a young healthy man.we report a case of splenic abscess with multiple brain abscesses caused by streptococcus intermedius in a healthy young man without any identifiable risk factors, which resolved with percutaneous drainage and antibiotics. streptococcus intermedius, a member of the streptococcus anginosus group, is a common commensal organism of the oral cavity and gastrointestinal tract, and it is a known cause of deep-seated infections. suppurative infections caused by streptococcus anginosus group are sometim ...201121738290
brain abscess due to streptococcus intermedius in a 3-year-old child. 201122181575
Cerebral abscess due to persistent left superior vena cava draining into the left atrium. 201122105199
Giant psoas abscess caused by Streptococcus intermedius. 201121847553
Extracellular DNA in biofilms.Extracellular DNA (eDNA) is an important biofilm component that was recently discovered. Its presence has been initially observed in biofilms of Pseudomonas aeruginosa, Streptococcus intermedius, Streptococcus mutans, then Enterococcus faecalis and staphylococci. Autolysis is the common mechanism by which eDNA is released. In P. aeruginosa eDNA is generated by lysis of a bacterial subpopulation, under control of quorum sensing system. In E. faecalis autolysis proceeds in a fratricide mode, resul ...201122094562
pathology in practice. bacterial pseudomycetoma. 201121879957
successful neuroendoscopic treatment of intraventricular brain abscess rupture.intraventricular rupture of a brain abscess is still associated with a high mortality rate. here, we report such a case in a patient with normal immunity that was treated successfully using neuroendoscopic approach. a 69-year-old man who had presented with headache and fever developed confusion and restlessness. magnetic resonance imaging revealed a mass with ring enhancement extending to the right ventricle. emergency aspiration of cerebrospinal fluid (csf) from the spinal canal revealed severe ...201124765313
polymicrobial subdural empyema: involvement of streptococcus pneumoniae revealed by lyta pcr and antigen detection.the authors report a case of a subdural empyema (sde) caused by a coinfection with streptococcus intermedius and streptococcus pneumoniae, initially considered a s. intermedius infection only. an otherwise healthy 11-year-old female was admitted to the hospital after 5 days of illness. symptoms were consistent with classical sde symptoms and progressed rapidly with finally somnolence before the first neurosurgical procedure despite relevant antibiotic treatment. primary mri showed an interhemisp ...201122707602
invasion of human aortic endothelial cells by oral viridans group streptococci and induction of inflammatory cytokine production.oral viridans group streptococci are the major commensal bacteria of the supragingival oral biofilm and have been detected in human atheromatous plaque. atherosclerosis involves an ongoing inflammatory response, reportedly involving chronic infection caused by multiple pathogens. the aim of this study was to examine the invasion of human aortic endothelial cells (haecs) by oral viridans group streptococci and the subsequent cytokine production by viable invaded haecs. the invasion of haecs by ba ...201021214874
sialidase of streptococcus intermedius: a putative virulence factor modifying sugar chains.a sialidase gene of streptococcus intermedius was cloned. it was most similar to nana, a major sialidase gene in streptococcus pneumoniae, and was expressed in escherichia coli. since the gene-knockout s. intermedius strain lost detectable sialidase activity, the gene might code, either solely or mainly, the glycosidase in the bacterial genome. polymerase chain reaction using the primers for the nana homologue in s. intermedius (described as nana below) showed that this sialidase gene was common ...201021118296
osteoradionecrosis contains a wide variety of cultivable and non-cultivable bacteria.direct microscopy, anaerobic culture and dna-dna hybridization have previously demonstrated an association between microorganisms and osteoradionecrosis (orn). the purpose of our study was to use culture independent molecular techniques to detect bacteria in necrotic bone lesions of the mandible after radiation therapy.201021523230
a high-affinity inhibitor of human cd59 enhances complement-mediated virolysis of hiv-1: implications for treatment of hiv-1/aids.many pathogenic enveloped viruses, including hiv-1, escape complement-mediated virolysis by incorporating host cell regulators of complement activation into their own viral envelope. the presence of complement regulators including cd59 on the external surface of the viral envelope confers resistance to complement-mediated virolysis, which may explain why human pathogenic viruses such as hiv-1 are not neutralized by complement in human fluids, even in the presence of high ab titers against the vi ...201019955519
characterization of streptococcus milleri group isolates from expectorated sputum of adult patients with cystic fibrosis.with the recent insights into the streptococcus milleri group (smg) as pulmonary pathogens in patients with cystic fibrosis (cf), we sought to characterize 128 isolates from the sputum of adults with cf, along with 45 isolates from patients with invasive diseases for comparison. the tests performed included lancefield grouping; tests for hemolysis; tests for the production of hyaluronidase, chondroitin sulfatase, dnase, proteases, and hydrogen peroxide; and pcr for the detection of the intermedi ...201020007382
diffusion-weighted mr imaging of intramedullary spinal cord abscess.early diagnosis and prompt initiation of adequate treatment are essential for clinical outcome in isca. we report a case in which dwi provided a more specific diagnosis than conventional mr imaging and allowed differentiation of a ring-enhancing lesion from intramedullary tumor. diagnosis was proved by pcr from csf (streptococcus intermedius). adequate antibiotic treatment was immediately initiated, and the patient recovered completely.201020019101
amplification of minute amounts of oral bacterial dna for real-time quantitative pcr analysis.high-throughput technologies for typing caries or health-associated bacterial populations including pcr, dna microarrays and next-generation sequencing techniques require significant amounts of bacterial dna. in clinical settings, the amount of sampled dna is often limited and amplification is therefore essential. protocols should be able to reproducibly amplify sequences in order to maintain initial sequence ratios and should not bias the representation of particular dna sequence types.201020881394
inerolysin, a cholesterol-dependent cytolysin produced by lactobacillus iners.lactobacillus iners is a common constituent of the human vaginal microbiota. this species was only recently characterized due to its fastidious growth requirements and has been hypothesized to play a role in the pathogenesis of bacterial vaginosis. here we present the identification and molecular characterization of a protein toxin produced by l. iners. the l. iners genome encodes an open reading frame with significant primary sequence similarity to intermedilysin (ily; 69.2% similarity) and vag ...201021169489
crystal structure of cytotoxin protein suilysin from streptococcus suis.cholesterol-dependent cytolysins (cdc) are pore forming toxins. a prototype of the cdc family members is perfringolysin o (pfo), which directly binds to the cell membrane enriched in cholesterol, causing cell lysis. however, an exception of this general observation is intermedilysin (ily) of streptococcus intermedius, which requires human cd59 as a receptor in addition to cholesterol for its hemolytic activity. a possible explanation of this functional difference is the conformational variation ...201021204001
acute subepicardial infarction associated with severe septic shock--insight in myocardial perfusion.isolated infarctions of the subepicardial myocardium without changes in subendocardium are extremely rare. we present an autoptic case with an acute subepicardial infarction of the right- and left-ventricular myocardium. a 53-year-old male was admitted to hospital with acute upper abdominal pain. clinical examination revealed an acute infero-lateral myocardial infarction. the patient succumbed to acute heart failure a few hours later. autopsy revealed numerous pulmonary abscesses due to suppurat ...201019945802
streptococcal bacteraemia in patients submitted to hematopoietic stem cell transplantation: the role of tooth brushing and use of chlorhexidine.a retrospective evaluation of 73 consecutive recipients of hematopoietic stem cell transplantation (hsct) was conducted to investigated the role of oral care and incidence of streptococcal bacteremia in patients submitted to hematopoietic stem cell transplantation. patients were retrospectively evaluated and divided into group a (ga=38) and group b (gb=35). during hospitalization patients from ga performed oral hygiene daily with extra soft toothbrush and toothpaste besides performing mouth clea ...201020038916
the cholesterol-dependent cytolysin family of gram-positive bacterial toxins.the cholesterol-dependent cytolysins (cdcs) are a family of beta-barrel pore-forming toxins secreted by gram-positive bacteria. these toxins are produced as water-soluble monomeric proteins that after binding to the target cell oligomerize on the membrane surface forming a ring-like pre-pore complex, and finally insert a large beta-barrel into the membrane (about 250 a in diameter). formation of such a large transmembrane structure requires multiple and coordinated conformational changes. the pr ...201020213558
a curious case of convulsion. 201020569845
unsuspected polymicrobial brain abscess arising from an intra-abdominal source in a patient with hereditary hemorrhagic telangiectasia.the unusual and unprecedented occurrence of a patient with hereditary hemorrhagic telangiectasia (hht) and a polymicrobial abscess with three different organisms, including fungi, is reported. the patient was a 48-year-old woman with human immunodeficiency virus (hiv) infection and hht who was brought to the hospital after a motor vehicle accident with altered mental status. computed tomography did not reveal evidence of acute brain injury but showed a left frontal brain abscess. the patient und ...201020622729
role of catabolite control protein a in the regulation of intermedilysin production by streptococcus intermedius.streptococcus intermedius is an opportunistic pathogen of humans that causes purulent infections, including brain and liver abscesses. this pathogen secretes a human-specific cytolysin, intermedilysin, which has been recognized as a major virulence factor. however, most of the expressional control mechanisms of ily are still unknown. to determine these mechanisms, we analyzed the nucleotide sequence of the ily promoter region. we found a highly homologous region to the catabolite-repressible ele ...201020624907
endophthalmitis caused by phialophora verrucosa and streptococcus intermedius: a case report.the most common pathogenic germs of mycotic endophthalmitis are candida, fusarium and aspergillus fumigatus. as a dematiaceous fungus, phialophora verrucosa (medlar, 1915) has not been reported to cause endophthalmitis. herein, we report a case of endophthalmitis induced by p. verrucosa and streptococcus intermedius.201020807026
descending necrotising mediastinitis: two case reports and review of the literature.descending necrotising mediastinitis is a rare and serious infection with a high mortality rate, which complicates pharyngeal or odontogenic infection. early recognition and treatment are essential in order to minimise morbidity. evaluation with computed tomography is necessary to confirm the diagnosis and facilitate surgical planning. in addition to prompt empirical antibiotic therapy, surgical intervention is necessary in nearly all cases. surgical drainage and debridement may be performed thr ...201020956183
photokilling of bacteria by curcumin in selected polyethylene glycol 400 (peg 400) preparations. studies on curcumin and curcuminoids, xli.curcumin, bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione, is a yellow-orange pigment which can be synthesised chemically or isolated from the plant curcuma longa l. curcumin has a rather broad absorption peak in the range 300-500 nm (maximum approximately 430 nm) and has potential as a photosensitiser for treatment of localised superficial infections in e.g., the mouth or skin. previously, we have demonstrated phototoxic effects of curcumin in selected aqueous preparations against both ...201020824961
identification of cultivable microorganisms from primary teeth with necrotic pulps.the objective of this study was to identify cultivable microorganisms from primary teeth with necrotic pulps. this experimental study included 21 patients of both sexes between 4 and 7 years of age with necrotic pulps in primary teeth. twenty-one maxillary and mandibular molars containing at least 1 necrotic canal, an abscess or sinus tract, one or more radiolucent areas in the furcation or periapical region, teeth having at least two thirds of root length, and carious lesions directly exposed t ...201020831135
spontaneous bacterial seeding of a biceps hematoma.a 19-year-old male construction worker presented with an injury to his left upper arm after lifting a heavy pipe. he reported an acute onset of sharp pain followed by swelling, warmth, and weakness with elbow flexion. the diagnosis of a distal biceps tendon rupture was made and elective repair was scheduled. seventy-two hours later, the patient presented with a spontaneous draining wound on his anterior distal humerus. the wound was draining thick purulent material. the patient underwent surgery ...201021053873
intermedilysin induces egr-1 expression through calcineurin/nfat pathway in human cholangiocellular carcinoma cells.intermedilysin (ily) is a cholesterol-dependent cytolysin produced by streptococcus intermedius, which is associated with human brain and liver abscesses. although intrahepatic bile duct cells play a valuable role in the pathogenesis of liver abscess, the molecular mechanism of ily-treated intrahepatic bile duct cells remains unknown. in this study, we report that ily induced a nuclear accumulation of intracellular calcium ([ca(2+)]i) in human cholangiocellular cells hucct1. we also demonstrate ...201021094139
the critical roles of platelet activation and reduced no bioavailability in fatal pulmonary arterial hypertension in a murine hemolysis model.pulmonary arterial hypertension (pah) is suspected to be a strong mortality determinant of hemolytic disorders. however, direct contribution of acute intravascular hemolysis to fatal pah has not been investigated. the roles of nitric oxide (no) insufficiency and platelet activation in hemolysis-associated fatal pah have been suspected but not been experimentally studied. we recently generated a unique intravascular hemolysis mouse model in which the membrane toxin, intermedilysin (ily), exclusiv ...201020511540
a streptococcal effector protein that inhibits porphyromonas gingivalis biofilm development.dental plaque formation is a developmental process involving cooperation and competition within a diverse microbial community, approximately 70 % of which is composed of an array of streptococci during the early stages of supragingival plaque formation. in this study, 79 cell-free culture supernatants from a variety of oral streptococci were screened to identify extracellular compounds that inhibit biofilm formation by the oral anaerobe porphyromonas gingivalis strain 381. the majority of the st ...201020705665
development of real-time pcr assays for detection of the streptococcus milleri group from cystic fibrosis clinical specimens by targeting the cpn60 and 16s rrna genes.cystic fibrosis (cf) is a multiorgan disease, with the majority of mortalities resulting from pulmonary failure due to repeated pulmonary exacerbations. recently, members of the streptococcus anginosus group (s. anginosus, s. constellatus, and s. intermedius), herein referred to as the "streptococcus milleri group" (smg) have been implicated as important etiological pathogens contributing to pulmonary exacerbations in cf patients. this is partly due to better microbiological detection of the smg ...201020164275
endodontic photoactivated disinfection using a conventional light source: an in vitro and ex vivo study.the antimicrobial effect of photoactivated disinfection (pad) using toluidine blue and an led lamp was tested on endodontic pathogens in planktonic suspension and after inoculation into extracted teeth. irradiation time was limited to 30 seconds.201020303060
mckay agar enables routine quantification of the 'streptococcus milleri' group in cystic fibrosis patients.the 'streptococcus milleri' group (smg) has recently been recognized as a contributor to bronchopulmonary disease in cystic fibrosis (cf). routine detection and quantification is limited by current cf microbiology protocols. mckay agar was developed previously for the semi-selective isolation of this group. here, mckay agar was validated against a panel of clinical smg isolates, which revealed improved smg recovery compared with columbia blood agar. the effectiveness of this medium was evaluated ...201020093379
viridans streptococcal (streptococcus intermedius) mitral valve subacute bacterial endocarditis (sbe) in a patient with mitral valve prolapse after a dental procedure: the importance of antibiotic prophylaxis.subacute bacterial endocarditis (sbe) is an infection of the heart involving damaged valves or endothelium. the most common organisms causing sbe are the viridans streptococci. viridans streptococci differ in their propensity to cause sbe, which is related to the ability to adhere to damaged heart valves and endothelium, which is a function of extracellular matrix production. streptococcus intermedius is a member of the s. anginosus group. s. intermedius is one of the many strains of viridans st ...201020109987
long-term bacterial exposure can trigger nonsuppurative destructive cholangitis associated with multifocal epithelial inflammation.bacterial infection has become a focus of attention in the pathogenesis of primary biliary cirrhosis (pbc). we earlier reported that the bacterial lipoteichoic acid was detected at the sites of inflammation around damaged bile ducts in the livers of pbc, and pbc patients' sera showed high titers against streptococcal histone-like protein. here, we investigated whether chronic bacterial exposure could trigger pbc-like epithelial cell damage in normal mouse. balb/c mice were repeatedly inoculated ...201020142809
multiple liver abscesses with isolation of streptococcus intermedius related to a pyogenic dental infection in an immuno-competent patient.introduction: streptococcus intermedius - a member of the streptococcus anginosus group - is part of the normal microbial flora of the oral cavity. despite being regarded as a harmless apathogenic commensal, streptococcus intermedius has been described to cause abscesses in various locations of the body. - case presentation: we report the clinical case and course of treatment of a 18-year-old male patient presenting with multiple hepatic abscesses associated with an untreated pyogenic dental inf ...201020696645
photoactivated disinfection of streptococcus intermedius through dentin disc at clinically relevant intervals: an in vitro study.in this present study we have tested the impact of porfimer sodium (photofrin, axcan pharma inc., quebec, canada) photoactivated disinfection (pd) on cells of streptococcus intermedius in suspension. in order to provide basic data to support future clinical studies of pd in dentistry the study used exposure to quartz-tungsten-halogen (qth) dental curing light for clinically relevant time periods to activate photofrin and measured its effectiveness under a variety of conditions including activati ...201020705280
intermedilysin-receptor interactions during assembly of the pore complex: assembly intermediates increase host cell susceptibility to complement-mediated lysis.intermedilysin (ily) is an unusual member of the family of cholesterol-dependent cytolysins because it binds to human cd59 (hcd59) rather than directly to cholesterol-rich membranes. binding of ily to hcd59 initiates a series of conformational changes within the toxin that result in the conversion of the soluble monomer into an oligomeric membrane-embedded pore complex. in this study the association of ily with its membrane receptor has been examined throughout the assembly and formation of the ...200919293153
identification of the high affinity binding site in the streptococcus intermedius toxin intermedilysin for its membrane receptor, the human complement regulator cd59.the unique species specificity of the bacterial cytolysin intermedilysin is explained by its requirement for the human complement regulator cd59 as the primary receptor. binding studies using individual domains of intermedilysin mapped the cd59-binding site to domain 4 and swap mutants between human and rabbit (non-intermedilysin-binding) cd59 implicated a short sequence (residues 42-59) in human cd59 in binding intermedilysin. we set out to map more closely the cd59 binding site in intermedilys ...200919200600
streptococcus intermedius-associated late-onset endophthalmitis after trabeculectomy with adjunctive mitomycin c.to report a woman with congenital rubella syndrome of delayed-onset, bleb-related endophthalmitis induced by streptococcus intermedius.200919142140
photokilling of bacteria by curcumin in different aqueous preparations. studies on curcumin and curcuminoids xxxvii.curcumin has potential as a photosensitiser (ps) in photodynamic therapy (pdt) for localised superficial infections. however, it is a challenge to make an optimal curcumin formulation in which curcumin has acceptable solubility and stability at physiological ph and combined with high selective phototoxic activity towards bacteria. in the present study, the phototoxic effects of curcumin against gram-negative and gram-positive bacteria were investigated in selected aqueous preparations. the gram- ...200919947170
ai-2/luxs is involved in increased biofilm formation by streptococcus intermedius in the presence of antibiotics.bacteria utilize quorum-sensing communication to organize their behavior by monitoring the concentration of bacterial signals, referred to as autoinducers (ais). the widespread detection of ai-2 signals and its enzymatic synthase (luxs) in bacteria suggests that ai-2 is an inter- and intraspecies communication signal. we have previously shown that antibiotic susceptibility is affected by ai-2 signaling in streptococcus anginosus. since chronic infections involve persistent biofilms resilient to ...200919596873
gene cloning and characterization of streptococcus intermedius fimbriae involved in saliva-mediated aggregation.streptococcus intermedius, an oral commensal and a cause of systemic pyogenic disease, expresses fimbriae. to identify the gene(s) encoding these fimbriae, we used a serum raised against purified fimbriae to screen libaries of recombinant lambda phages. the cloned gene cluster encoding s. intermedius fimbriae, (saliva-mediated aggregation and adherence-associated fimbriae), contained 4 orfs, i.e. a putative ribonulease (saf1), a putative adhesin (saf2), the main pilus subunit (saf3) and a sortas ...200919819330
multiple brain abscesses associated with tongue piercing. 200919822789
spontaneous cranial osteomyelitis in an otherwise healthy ten-year-old male.frontal bone osteomyelitis is a relatively rare entity, particularly in the otherwise healthy pediatric population. most cases trace their origins to either previous frontal sinusitis or trauma. in children, three origins for cranial osteomyelitis appear to dominate: pott's puffy tumor, skull base osteomyelitis secondary to ear infection, or post-surgical complications. however, on extremely rare occasions, risk factors or etiology may not be ascertained. we present a case of spontaneous frontal ...200920051698
evaluation of genotypic and phenotypic methods for differentiation of the members of the anginosus group streptococci.the terminology and classification of the anginosus group streptococci has been inconsistent. we tested the utility of 16s rrna gene and tuf gene sequencing and conventional biochemical tests for the reliable differentiation of the anginosus group streptococci. biochemical testing included rapid id 32 strep, api strep, fluo-card milleri, wee-tabs, and lancefield antigen typing. altogether, 61 anginosus group isolates from skin and soft tissue infections and four reference strains were included. ...200919495818
brain abscesses in a patient with a patent foramen ovale: a case report.brain abscesses arising from right-to-left cardiac shunting are very rare in adults.200920062788
does pregnancy have an impact on the subgingival microbiota?we investigated clinical and subgingival microbiologic changes during pregnancy in 20 consecutive pregnant women > or =18 years not receiving dental care.200919228092
cellular functions and x-ray structure of anthrolysin o, a cholesterol-dependent cytolysin secreted by bacillus anthracis.anthrolysin o (alo) is a pore-forming, cholesterol-dependent cytolysin (cdc) secreted by bacillus anthracis, the etiologic agent for anthrax. growing evidence suggests the involvement of alo in anthrax pathogenesis. here, we show that the apical application of alo decreases the barrier function of human polarized epithelial cells as well as increases intracellular calcium and the internalization of the tight junction protein occludin. using pharmacological agents, we also found that barrier func ...200919307185
streptococcus constellatus bacteremia causing septic shock following tooth extraction: a case report.organisms of the streptococcus milleri group consists of streptococcus intermedius, streptococcus constellatus, and streptococcus anginosus. though they are commonly associated with abscesses, bacteremia with subsequent septic shock and mortality is infrequent.200919829816
histone-like dna binding protein of streptococcus intermedius induces the expression of pro-inflammatory cytokines in human monocytes via activation of erk1/2 and jnk pathways.streptococcus intermedius is a commensal associated with serious, deep-seated purulent infections in major organs, such as the brain and liver. histone-like dna binding protein (hlp) is an accessory architectural protein in a variety of bacterial cellular processes. in this study, we investigated the mechanisms of pro-inflammatory cytokine inductions in thp-1 cells by stimulation with recombinant hlp of s. intermedius (rsi-hlp). rsi-hlp stimulation-induced production of pro-inflammatory cytokine ...200817883418
analysis of the antimicrobial activity of local anaesthetics used for dental analgesia.seven local anaesthetics and their active anaesthetic components [ultracaine d-s (articaine hydrochloride), carbostesin (bupivacaine hydrochloride), scandicaine (mepivacaine hydrochloride), xylonest (prilocaine hydrochloride), xylocaine (lidocaine hydrochloride), hostacaine (butanilicaine phosphate) and novocaine (procaine hydrochloride)] were tested for their antimicrobial activity against 311 bacterial strains from 52 different species and 14 candida albicans strains. the tested pathogens were ...200818065672
functional and phylogenetic characterization of vaginolysin, the human-specific cytolysin from gardnerella vaginalis.pore-forming toxins are essential to the virulence of a wide variety of pathogenic bacteria. gardnerella vaginalis is a bacterial species associated with bacterial vaginosis (bv) and its significant adverse sequelae, including preterm birth and acquisition of human immunodeficiency virus. g. vaginalis makes a protein toxin that generates host immune responses and has been hypothesized to be involved in the pathogenesis of bv. we demonstrate that g. vaginalis produces a toxin (vaginolysin [vly]) ...200818390664
pcr-based identification of selected pathogens associated with endodontic infections in deciduous and permanent teeth.the aim of the present study was to evaluate the presence of the selected pathogens in samples from deciduous and permanent tooth root canals by using pcr method and to determine the association of these organisms with clinical symptoms.200818547832
characterization of a streptococcal cholesterol-dependent cytolysin with a lewis y and b specific lectin domain.the cholesterol-dependent cytolysins (cdcs) are a large family of pore-forming toxins that often exhibit distinct structural changes that modify their pore-forming activity. a soluble platelet aggregation factor from streptococcus mitis (sm-hpaf) was characterized and shown to be a functional cdc with an amino-terminal fucose-binding lectin domain. sm-hpaf, or lectinolysin (lly) as renamed herein, is most closely related to cdcs from streptococcus intermedius (ily) and streptococcus pneumoniae ( ...200818553932
streptococcus cristatus attenuates fusobacterium nucleatum-induced interleukin-8 expression in oral epithelial cells.oral epithelial cells may be invaded by a polymicrobial intracellular flora, including pathogens together with commensals. various oral pathogens can induce the production of interleukin-8, a potent neutrophil chemotractant, in oral epithelial cells. evidence from the gut suggests that commensal species may modulate inflammatory responses to pathogens. the aim of this study was to examine the interleukin-8 responses of oral epithelial cells to an oral pro-inflammatory species, fusobacterium nucl ...200818942189
biofilm formation and autoinducer-2 signaling in streptococcus intermedius: role of thermal and ph factors.bacteria sense their population density using autoinducer (ai) signaling systems. the ai-2 signal is thought to mediate communication among and within bacterial species. streptococcus intermedius is a commensal organism frequently associated with periodontitis and purulent infections. we investigated the role of ai-2 signaling in s. intermedius biofilm formation under temperatures and ph levels relevant to human physiology.200818954356
streptococcus intermedius causing infective endocarditis and abscesses: a report of three cases and review of the literature.streptococcus intermedius is a member of the streptococcus anginosus group. clinical disease with s. intermedius is characterized by abscess formation and rarely endocarditis. identification of streptococcus intermedius is difficult, leading to the development of molecular methods to more accurately identify and characterize this organism.200818992173
streptococcus mutans and streptococcus intermedius adhesion to fibronectin films are oppositely influenced by ionic strength.bacterial adhesion to protein-coated surfaces is mediated by an interplay of specific and nonspecific interactions. although nonspecific interactions are ubiquitously present, little is known about the physicochemical mechanisms of specific interactions. the aim of this paper is to determine the influence of ionic strength on the adhesion of two streptococcal strains to fibronectin films. streptococcus mutans lt11 and streptococcus intermedius nctc11324 both possess antigen i/ii with the ability ...200818729493
heterologous expression ofa histone-like protein from streptococcus intermedius in escherichia coli alters the nucleoid structure and inhibits the growth of e. coli.escherichia coli failed to survive after transformation with a streptococcus intermedius histone-like protein gene (si-hlp) and its promoter-harbored plasmid. the promoter function of si-hlp in e. coli was determined using enhanced green fluorescence protein (egfp) gene as a reporter. the inhibitory effect of si-hlp on e. coli viability was verified by a tetracycline-inducible gene expression system. further study suggested that si-hlp may alter the bacterial nucleoid structure, leading to the g ...200818761672
identification and molecular analysis of betac-s lyase producing hydrogen sulfide in streptococcus intermedius.hydrogen sulfide (h(2)s) is a toxic gas that induces the modification and release of haemoglobin in erythrocytes; however, it also functions in methionine biosynthesis in bacteria. betac-s lyase, encoded by the lcd gene, is responsible for bacterial h(2)s production through the cleavage of l-cysteine. in this study, 26 of 29 crude extracts from reference and clinical strains of streptococcus intermedius produced h(2)s from l-cysteine. the capacities in those strains were not higher than those in ...200818927421
[when genetics matters: an unusual case of left-sided hemiparesis].we report the case of a 35-year-old woman who has been admitted to our emergency room because of the sudden onset of a left-sided hemiparesis. the physical examination showed disseminated teleangiectases on the upper and lower lip, on the mucosal surface of the tongue and on the skin. the cerebral ct scan presented a right-sided fronto-parietal lesion, more likely an abscess, confirmed on surgical removal. a meticulous family history showed the presence of similar clinical features among several ...200819016425
pott's puffy tumor and epidural abscess arising from pansinusitis.background: sinusitis is a common disorder that can result in rare but serious complications including periorbital or orbital cellulitis, intracranial abscess or meningitis, subperiosteal scalp abscess ("pott's puffy tumor"), osteomyelitis, and cavernous sinus thrombosis. case report: we report a case of a 41-year-old man who presented to our emergency department with pansinusitis. he did not obtain recommended follow-up treatment after discharge and 26 days later returned with a persistence of ...200819022612
rapid conditional targeted ablation of cells expressing human cd59 in transgenic mice by intermedilysin.conditional targeted cell ablation is a powerful approach for investigating the pathogenesis of human diseases and in vivo cellular functions. intermedilysin (ily) is a cytolytic pore-forming toxin secreted by streptococcus intermedius that lyses human cells exclusively, owing to its receptor specificity for human cd59. we generated two transgenic mouse strains that express human cd59 either on erythrocytes (strain thcd59(rbc)) or on endothelia (strain thcd59(end)). intravenous injection of ily ...200818157141
luxs and expression of virulence factors in streptococcus intermedius.autoinducer-2 (ai-2) is used by several bacteria in quorum-sensing signaling and is a product of luxs. the aim was to investigate the effect of luxs mutation on expression of streptococcus intermedius virulence factors.200818173802
role of hyaluronidase in streptococcus intermedius biofilm.streptococcus intermedius is found in biofilms on teeth and as a commensal member of the gastrointestinal and urinary floras, but may also be associated with deep-seated purulent infections and infective endocarditis. s. intermedius produces hyaluronidase, an enzyme that breaks down hyaluronan (ha), a major component of the extracellular matrix of connective tissue. we investigated the involvement of hyaluronidase in s. intermedius biofilm formation and dispersal as well as adhesion to human cel ...200818310039
a possible role of histone-like dna-binding protein of streptococcus intermedius in the pathogenesis of bile duct damage in primary biliary cirrhosis.bacterial infection has become a focus of attention in the pathogenesis of primary biliary cirrhosis (pbc). it was reported that anti-histone autoantibody was detected in pbc, suggesting that bacterial histone-like dna-binding protein (hlp) may be involved in the pathogenesis of pbc. to identify bacterial species in pbc to confirm this possibility, serum reactivity to bacterial cells was studied by elisa. the igm class streptococcus intermedius titers were significantly higher in pbc than chroni ...200818337173
the essentiality and involvement of streptococcus intermedius histone-like dna-binding protein in bacterial viability and normal growth.streptococcus intermedius histone-like dna-binding protein (si-hlp) is a homodimeric protein and, conserved with escherichia coli hu, a well-documented nucleoid-associated protein (nap). in e. coli, hu plays important roles as both structural and regulatory factors, but it is not essential for e. coli viability. streptococcal hlp has been found to bind host cells and induce cytokine production, but its physiological role remains poorly defined. in the present study, using gene insertion knockout ...200818410499
electronic clinical challenges and images in gi. pyogenic liver abscesses caused by an infection with streptococcus intermedius (streptococcus milleri group). 200819000680
spondylodiscitis and infectious endocarditis: a round-trip to be considered.the association between spondylodiscitis and endocarditis was first reported in 1965 by de sèze et al. the most common clinical picture of this association is musculoskeletal symptoms preceding endocarditis diagnosis, but we report here a case of spondylodiscitis complicating endocarditis in its late course. a 70-year-old man, with an established diagnosis of mitral valve endocarditis caused by streptococcus intermedius, early submitted to surgical treatment because of heart failure, who had an ...200819287848
[virulence factors of anginosus group streptococci]. 200819317232
detection of porphyromonas gingivalis and streptococcus intermedius in chronic periodontitis patients by multiplex pcr.a multiplex pcr assay for the detection of porphyromonas gingivalis and streptococcus intermedius in chronic periodontitis is presented. a total of 180 samples from 65 adults with untreated periodontitis and 17 healthy volunteers were taken and processed in a simple boiling step. cell lysates were used as dna source for multiplex pcr assays. primers were designed from 16s rrna gene sequences from the genbank-embl database showing specificity for target pathogens. this multiplex pcr system could ...200819177854
assessment of the microbiota in root canals of human primary teeth by checkerboard dna-dna hybridization.the purpose of this study was to evaluate in vivo the prevalence of microorganisms in root canals of human primary teeth, by checkerboard dna-dna hybridization.200718477431
acetaldehyde production from ethanol by oral streptococci.alcohol is a well documented risk factor for upper digestive tract cancers. it has been shown that acetaldehyde, the first metabolite of ethanol is carcinogenic. the role of microbes in the production of acetaldehyde to the oral cavity has previously been described in several studies. in the present study, the aim was to investigate the capability of viridans group streptococci of normal oral flora to produce acetaldehyde in vitro during ethanol incubation. furthermore, the aim was to measure th ...200716859955
structural elements of the cholesterol-dependent cytolysins that are responsible for their cholesterol-sensitive membrane interactions.the pore-forming mechanism of the cholesterol-dependent cytolysins (cdcs) exhibits an absolute requirement for membrane cholesterol. the structural elements of the cdcs that mediate this interaction are not well understood. three short hydrophobic loops (l1-l3) and a highly conserved undecapeptide sequence at the tip of domain 4 of the cdc structure are known to anchor the cdc to the membrane. it has been thought that the undecapeptide directly mediates the interaction of the cdcs with a cholest ...200718077338
splenic abscess in an infant caused by streptococcus intermedius.we report a 20-month-old girl with splenic abscess. the patient was admitted to our hospital because of persistent high fever and abdominal pain. laboratory data showed leucocytosis and elevated c-reactive protein levels. abdominal computed tomography showed multiple low-density lesions in the spleen. these findings were consistent with a diagnosis of splenic abscess. she was successfully treated with ultrasonographically guided percutaneous drainage for 11 days and intravenous antibiotic for 17 ...200718095094
lemierre syndrome associated with dental infections. report of one case and review of the literature.the first publication on lemierre syndrome appears in 1936 by lemierre. it is defined as an "oropharynx bacterial infection characterized by the thrombophlebitis in the internal jugular vein, derived in a systemic septic embolism". in 81% of the cases, the fusobacterium necrophorum is the most frequent etiologic agent. fever is the most common symptom, but it can depending on the primary infection, tonsillitis, mastoiditis or odontogenic infection. according to the literature the mortality is ve ...200717767106
specific protein-membrane contacts are required for prepore and pore assembly by a cholesterol-dependent cytolysin.three short hydrophobic loops and a conserved undecapeptide at the tip of domain 4 (d4) of the cholesterol-dependent cytolysins (cdcs) mediate the binding of the cdc monomers to cholesterol-rich cell membranes. but intermedilysin (ily), from streptococcus intermedius, does not bind to cholesterol-rich membranes unless they contain the human protein cd59. this observation suggested that the d4 loops, which include loops l1-l3 and the undecapeptide, of ily were no longer required for its cell bind ...200717412689
conformational changes that effect oligomerization and initiate pore formation are triggered throughout perfringolysin o upon binding to cholesterol.pore formation by the cholesterol-dependent cytolysins (cdcs) requires the presence of cholesterol in the target membrane. cholesterol was long thought to be the cellular receptor for these toxins, but not all cdcs require cholesterol for binding. intermedilysin, secreted by streptococcus intermedius, only binds to membranes containing the human protein cd59 but forms pores only if the membrane contains sufficient cholesterol. in contrast, perfringolysin o (pfo), secreted by clostridium perfring ...200717553799
primary ventriculitis caused by streptococcus intermedius.streptococcus intermedius is increasingly being recognised as an aetiological agent in central nervous system infections. primary ventriculitis caused by this organism has not been reported so far. we present a case of primary ventriculitis, which resulted in adhesions and multiloculated hydrocephalus, necessitating numerous surgical procedures to control it. no predisposing factor(s) could be identified. although the organism could not be cultured from csf, as he was already on antibiotic treat ...200717612921
orbital abscess after extraction of a maxillary wisdom tooth.a 21-year-old patient with orbital abscess and vision loss left after wisdom-tooth extraction is presented.200716310905
thigh abscess caused by eikenella corrodens and streptococcus intermedius: a case report.a 35-year-old chinese female has a large thigh abscess without a clear source. no underlying diseases were revealed in the patient. the combination of clindamycin and cefazolin or vancomycin was administered but without a clinical response. eikenella corrodens and streptococcus intermedius were isolated from puncture drainage. then, ceftriaxone was administered and a surgery of incision and further draining was carried. eventually, the patient recovered. to our knowledge, this is the first case ...200716682079
alternative roles for cd59.cd59 was first identified as a regulator of the terminal pathway of complement, which acts by binding to the c8/c9 components of the assembling membrane attack complex (mac), to inhibit formation of the lytic pore. structurally, cd59 is a small, highly glycosylated, gpi-linked protein, with a wide expression profile. functionally, the role of cd59 in complement regulation is well-defined but studies have also shown clear evidence for signalling properties, which are linked to its glycophosphatid ...200716884774
Refresh
firstprev
12345
nextlast
Displaying items 101 - 200 of 485