Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| structures and stereochemistry of the very long alpha, omega-bifunctional alkyl species in the membrane of sarcina ventriculi indicate that they are formed by tail-to-tail coupling of normal fatty acids. | in a previous study, we demonstrated that sarcina ventriculi is capable of adjusting to alterations in environmental conditions (such as increase in temperature, lowering of ph, or addition of exogenous organic solvents) by the synthesis of a family of alpha, omega-dicarboxylic acids ranging from 28 to 36 carbons long (jung, s., et al. 1993. j. biol. chem. 268: 2828-2835). the chain lengths and relative abundance of the very long dicarboxylic acids found in s. ventriculi suggest that they may be ... | 1994 | 7868972 |
| phylogenetic placement of sarcina ventriculi and sarcina maxima within group i clostridium, a possible problem for future revision of the genus clostridium. request for an opinion. | the 16s rrna gene sequences of sarcina ventriculi dsm 286t (t = type strain) and sarcina maxima dsm 316t were determined. phylogenetic analysis revealed that these two species are closely related to each other and belong to group i clostridium (sensu johnson and francis). the implications of these phylogenetic findings for future revision of the genus clostridium are discussed. | 1994 | 7520746 |
| isolation and physico-chemical characterization of an antifungal and antibacterial peptide produced by bacillus licheniformis a12. | an antifungal substance named peptide a12-c has been purified to homogeneity from supernatants of sporulated cultures of bacillus licheniformis a12. it consists of a 0.77-kda hydrophilic peptide containing two residues of glu and one of arg, ala, pro, tyr and orn. no fatty acids, phosphorus or carbohydrates have been detected. peptide a12-c is active on several fungi (microsporum canis cect 2797, mucor mucedo cect 2653, m. plumbeus (ccm f 443, sporothrix schenckii cect 2799 and trichophyton ment ... | 1993 | 7763922 |
| detection of beta-lactamase production and enzyme profiles in enterobacteriaceae isolated in moldova. | in our study, out of 449 enterobacteriaceae strains isolated between 1985 and 1990, 16 strains (3 proteus, 6 nontyphoidal salmonella, 7 escherichia coli) were resistant both to ampicillin- sulbactam and amoxycillin-clavulanic acid associations. the activity profiles of the beta-lactamases produced by these resistant strains are described. sarcina lutea atcc 9341 was used as test strain. the effect of the enzymatic filtrate against beta-lactam antibiotics: ampicillin, cloxacillin, cefadroxil, cef ... | 1993 | 8153468 |
| prevalence of self-medication with antibiotics among patients attending a clinic for treatment of sexually transmitted diseases. | to ascertain the prevalence of self-medication with antimicrobial agents among patients attending a clinic for treatment of sexually transmitted diseases (stds), we administered a questionnaire to and collected a urine specimen for antimicrobial testing from 551 patients before treatment. we defined self-medication as an antimicrobial agent taken on the patient's own initiative by self-report during the week before the visit to the clinic or a positive urine assay for antimicrobial agents at the ... | 1993 | 8218690 |
| new heterocyclic structures. thiazolo[3,2-a][1,2,5] thiadiazolo[3,4-d]pyrimidine and [1,2,5]thiadiazolo[3',4':4,5] pyrimido[2,1-b][1,3]thiazine. biological assays. | 6,7-dihydro-9h-thiazolo[3,2-a][1,2,5]thiadiazolo [3,4-d]pyrimidin-9-one, 7,8-dihydro-6h,10h-[1,2,5]thiadiazolo[3',4':4,5]pyrimido [2,1-b][1,3]thiazin-10-one and its 3-methyl derivative were prepared by reacting 6,7-diamino-2,3-dihydro-5h-thiazolo[3,2-a]pyrimidin-5-one, 7,8-diamino-3,4-dihydro-2h,6h-pyrimido[2,1-b][1,3]thiazin-6-one or its 3-methyl derivative with n-thionylaniline. a reaction mechanism is proposed. the compounds and the sodium salts of (7-amino-2,3-dihydro-5h-thiazolo[3,2-a]pyrim ... | 1993 | 8323673 |
| penetration of rifampicin into the brain tissue and cerebral extracellular space of rats. | rifampicin is used to treat neurosurgical shunt infections because of its excellent in-vitro activity against staphylococci and its adequate penetration into the csf. however, nothing is known about rifampicin concentrations in the cerebral extra-cellular space (ces). we measured the penetration of rifampicin into the ces of anaesthetized rats by microdialysis using low-flow and equilibrium methods. depending on the method, rifampicin concentrations in the ces were 0.3-1% of the serum concentrat ... | 1993 | 8335500 |
| sarcina ventriculi synthesizes very long chain dicarboxylic acids in response to different forms of environmental stress. | changes in the composition of membrane lipids in a strictly anaerobic, facultative acidophilic eubacterium, sarcina ventriculi, were studied in response to various forms of environmental stress. changes in lipid composition and structure occurred in response to changes in environmental ph. at neutral ph, the predominant membrane fatty acids ranged in chain length from c14 to c18. however, when cells were grown at ph 3.0, a family of unique very long chain fatty acids containing 32-36 carbon atom ... | 1993 | 8428957 |
| prospective evaluation of the incidence of bacteremia after protected specimen brushing in icu patients with and without pneumonia. | to test the hypothesis that the use of protected specimen brushing (psb) via flexible bronchoscopy does not predispose to bacteremia in icu patients, we prospectively performed aerobic and anaerobic blood cultures immediately following bronchoscopy with psb. a total of 123 episodes in 68 consecutive patients with suspected pneumonia were analyzed. blood cultures were negative in 110 cases (89 percent) and positive in 13 cases (11 percent) (p < 0.001). twelve of these 13 patients with positive bl ... | 1993 | 8432123 |
| purification, characterization, and lytic activity against naegleria fowleri of two amoebicins produced by bacillus licheniformis a12. | bacillus licheniformis a12 produces two amoebolytic substances (amoebicins a12-a and a12-b) in liquid media during sporulation. both substances have been purified and characterized. they are heat- and protease-resistant peptides containing aspartic acid, glutamic acid, serine, proline, and tyrosine in a molar ratio of 5:2:2:2:2. no fatty acids or carbohydrates have been detected. their molecular weight is 1,430. purified amoebicins a12-a and a12-b exhibit amoebolytic action against naegleria fow ... | 1993 | 8517742 |
| investigation of the causes facilitating formation of microflora in atomic reactor pool water. | in the course of investigations realized by us earlier it was found that there was no difference between radioresistance of microbes taken from various water sources. as a matter of fact quality of the microflora clearly reflects a unique phenomenon called selection which causes disappearance of all radiosensitive and survival of radioresistant types of microbes. there is indeed an increased number of radioresistant types of microbes with intensified activity of catalase and nuclease in pool wat ... | 1992 | 1338637 |
| cloning and expression of midecamycin 4"-acylase gene in spiramycin producing strains. | a recombinant plasmid p66b containing the midecamycin 4"-acylase gene was obtained by cloning this gene into plasmid vetor pij680 from the primary clone pcn6c5, presumably harboring the midecamycin biosynthetic gene. the expression of the midecamycin 4"-acylase gene (p66b) in spiramycin producing strains resulted mainly in the production of 4"-isovalerylspiramycin. another positive clone pcn10f5 was discovered from the genomic library of s. mycarofacians 1748 by probing with p66b dna bamhi-bamhi ... | 1992 | 1457716 |
| synthesis and biological activity of new heterocyclic structures: [1,3]thiazino[2,3-i]purine, thiazolo[3,2-c] [1,2,3]triazolo[4,5-e]pyrimidine and [1,2,3]triazolo [4', 5': 4,5] pyrimido[6,1-b][1,3]thiazine. | some derivatives of thiazolo[3,2-c]pyrimidine, pyrimido[6,1-b][1,3]thiazine, thiazolo[2,3-i]purine, [1,3]thiazino[2,3-i]purine, thiazolo[3,2-c][1,2,3]triazolo[4,5-e]pyrimidine and [1,2,3]triazolo[4',5':4,5]pyrimido[6,1-b][1,3]thiazine were prepared. the compounds were tested for antimicrobial and antimycotic activity on a number of strains, namely, escherichia coli, proteus vulgaris, p. mirabilis, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus, streptococcus faecalis, bacillus sub ... | 1992 | 1482518 |
| effect of penicillin on the clinical course of streptococcal pharyngitis in general practice. | the aim of the study was to explore whether penicillin was superior to placebo in altering the clinical course of proven streptococcal pharyngitis. a randomised, parallel, double blind placebo controlled trial of 10 days duration was undertaken in 42 general practices in the gent region (flemish part of belgium). phenoxymethylpenicillin (adults 250 mg t.i.d. and children 125 mg t.i.d.) or placebo were administrated to 173 patients, aged 5 to 50 y, with acute sore throat and a positive culture fo ... | 1992 | 1493837 |
| biological inactivation by singlet oxygen: distinguishing o2(1 delta g) and o2(1 sigma g+) | experiments were performed to determine whether bacterial inactivation in the separated-surface-sensitizer system for singlet oxygen generation is due to o2(1 delta g) or o2(1 sigma g+). the rates of inactivation of gram-negative salmonella typhimurium lt-2 and a nonpigmented strain of gram-positive sarcina lutea were found to increase linearly with the concentration of 1 delta g. the gas phase lifetime of the inactivating agent was found to be within the range of values expected for the gas pha ... | 1992 | 1525183 |
| in vitro hydrogen production by enteric bacteria cultured from children with small bowel bacterial overgrowth. | lactulose breath hydrogen test and enterotest string test were carried out simultaneously on 19 children 3-5 years old. bacteria isolated from the jejunal fluid in upper small intestines of these children were incubated with lactulose at neutral ph. anaerobes were present in all but one child, and in 15 children they were present in numbers greater than 5 log10 organisms per ml. most of these bacteria did not produce hydrogen in vitro. hydrogen production (greater than 100 ppm) was observed with ... | 1992 | 1534365 |
| purification and characterization of pyruvate decarboxylase from sarcina ventriculi. | pyruvate decarboxylase from the obligate anaerobe sarcina ventriculi was purified eightfold. the subunit mr was 57,000 +/- 3000 as estimated from sds-page, and the native mr estimated by gel filtration on a superose 6 column was 240,000, indicating that the enzyme is a tetramer. the mr values are comparable to those for pyruvate decarboxylase from zymomonas mobilis and saccharomyces cerevisiae, which are also tetrameric enzymes. the enzyme was oxygen stable, and had a ph optimum within the range ... | 1992 | 1588311 |
| comparison of automated liquid chromatographic and bioassay methods for determining spiramycin concentration in bovine plasma. | the performance of a liquid chromatographic (lc) method for spiramycin measurement in bovine plasma has been compared with that of a microbiological method. plasma samples were obtained from cattle administered spiramycin intravenously. comparison tests used were intraclass correlation (r1), correlation (r), and student's paired t-test. for concentrations lower than 2.5 iu/ml, microbiological values were higher than lc values. this difference in results modified pharmacokinetic interpretation an ... | 1991 | 1757414 |
| high-performance liquid chromatographic determination of rifapentine and its metabolite in human plasma by direct injection into a shielded hydrophobic phase column. | a simple high-performance liquid chromatographic method for determination of rifapentine, a cyclopentyl semisynthetic analogue of rifamycin belonging to the class of piperazinyl hydrazone derivatives of 3-formylrifamycin sv, and its metabolite, 25-desacetylrifapentine, in human plasma was developed using direct injection of the sample onto a supelco lc hisep column. the mean recovery was 100.3% for rifapentine and 99.7% for the metabolite and the precision of the assays was 3% and 7%, respective ... | 1991 | 1787163 |
| synthesis and biological activity of pyrimido[2,1-b][1,3]thiazine, [1,3]thiazino[3,2-a]purine and [1,2,3]triazolo[4,5-d][1,3]thiazino[3,2-a]pyrimidine derivatives and thiazole analogues. | some series of thiazolo[3,2-a]pyrimidine, pyrimido[2,1-b] [1,3]thiazine, thiazolo[3,2-a]purine, [1,3]thiazino[3,2-a]purine, thiazolo[3,2-a][1,2,3]triazolo[4,5-d]pyrimidine and [1,2,3]triazolo[4,5-d][1,3]thiazino[3,2-a]pyrimidine derivatives, variously functionalized, were prepared. the compounds were tested for antimicrobial and antimycotic activity on a number of strains, namely: e. coli, proteus vulgaris, p. mirabilis, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus, s. faecalis, ... | 1991 | 1793474 |
| penicillin amidohydrolase productivity of locally isolated bacterial species. | penicillin amidohydrolase productivity of four locally isolated bacterial species is described. organisms were identified as escherichia coli, pseudomonas aeruginosa, sarcina lutea and bacillus megaterium. highest enzyme productivity of 3.2 u/ml with a corresponding dry cell mass of 4.5 g/l was recorded from s. lutea. | 1991 | 1821869 |
| [biological assay, reliability test, error analysis and pooled calculation for assessing leucomycin yield]. | a biological assay for determining leucomycin yield was established by using a curillinear protocol (sarcina lutea) and (2.2) dosage or (3.3) dosage protocol (bacillus subtillis 63501). at the same time, according to the principles of biostatistics and the methods of variance analysis, reliability tests, error analysis and pooled calculations were carried out, so as to hold the confidence limit below the internationally accepted level of 5%. | 1991 | 1839519 |
| [shigella endotoxin protein--its electrophoretic and serological properties]. | the electrophoretic analysis of lipid a-associated protein (lap), obtained from s. sonnei, in polyacrylamide gel in the presence of sodium dodecyl sulfate and urea has revealed the heterogeneity of the preparation; it has found to contain three main components with molecular weights of 43, 38 and 18 kd and some minor components with molecular weights of 49, 45 35, 30, 29, 27, 5, 21 and 14 kd. the electrophoretic mobility of the main protein components in the isolated preparation of lap coincides ... | 1991 | 1882599 |
| bactericidal effect of 15-deoxyspergualin on staphylococcus aureus. | 15-deoxyspergualin (dsg), an immunosuppressive agent used in organ transplantation, exerts metabolic and antiproliferative effects on methicillin- and gentamicin-resistant staphylococcus aureus and other bacteria (e.g. sarcina lutea, bacillus subtilis, shigella sonnei, salmonella typhi and citrobacter freundii). dsg, at the concentration of 20 mg/l, depleted intracellular putrescine and spermidine in s. aureus to 43 and 40% of the controls, respectively. in these polyamine-depleted s. aureus cel ... | 1991 | 1889307 |
| some pharmacokinetic data on miocamycin ii. concentrations in gynaecological tissues. | the gynaecological tissue levels of miocamycin were studied in ten female patients after pre-operative administration. the patients were divided into two groups on the basis of the scheduled sampling time. all the patients received 4200 mg of the drug, divided in 7 tablets of 600 mg each every 8 h, last administration 2 or 3 h before surgery. the tissue levels, obtained by means of the microbiological method (sarcina lutea 9341), were frequently higher than those obtained in the serum at the sam ... | 1991 | 1914845 |
| kinetic profile of miocamycin in middle ear fluid and mucosa. | a group of 18 patients, before undergoing otoplastic surgery, was treated with miocamycin 600 mg in a single dose; middle ear tissue samples were collected at 2, 3, 4 and 6 h. a group of 20 patients suffering from secretory otitis media (som) was also treated with 600 mg of miocamycin in a single dose; collections of middle ear fluid (mef) were performed at 1, 2, 4 and 6 h. blood withdrawals from all patients occurred at the same time intervals. the miocamycin determination was performed by mean ... | 1991 | 1950539 |
| cellulose biosynthesis and function in bacteria. | the current model of cellulose biogenesis in plants, as well as bacteria, holds that the membranous cellulose synthase complex polymerizes glucose moieties from udp-glc into beta-1,4-glucan chains which give rise to rigid crystalline fibrils upon extrusion at the outer surface of the cell. the distinct arrangement and degree of association of the polymerizing enzyme units presumably govern extracellular chain assembly in addition to the pattern and width of cellulose fibril deposition. most evid ... | 1991 | 2030672 |
| female genital tissue concentrations of roxithromycin. | a new macrolide, roxithromycin, appears to have some interesting pharmacokinetic characteristics dissimilar to those of erythromycin, e.g. it has the unusual property of a long serum half-life and the possibility of once daily dosing. moreover tissue levels are higher than those obtained with other macrolides, and the drug is still present in the tissues up to 24 h. after dosing. the pharmacokinetics of roxithromycin were studied in 36 women undergoing gynecological surgery, and divided by means ... | 1991 | 12041780 |
| miocamycin distribution in tonsillar and pulmonary tissues after repeated administration. | twenty patients undergoing tonsillectomy were treated with a peroral administration of 600 mg of miocamycin every 12 hours for 4 days. on the 5th day, after a last administration of a dose of 600 mg the ablation of the tonsils was carried out on groups of 4 subjects, each one at the following times after oral intake of the drug: 2, 4, 6, 8, 12 h. twenty-nine patients, admitted to hospital to undergo lung resection were treated with peroral administration of miocamycin in accordance with the abov ... | 1990 | 2093111 |
| polar lipids of non-alkaliphilic halococci. | until recently, only one species of halococcus has been recognized, namely, h. morrhuae, but a large number of extremely halophilic non-alkaliphilic cocci have now been isolated from hypersaline habitats in spain and classified into four phenons (a-d); one of the phenon d strains has been classified as a new species, halococcus saccharolyticus. examination of the lipids of h. saccharolyticus and four strains of phenons a-c showed the presence in all of them of c20-c20 and c20-c25 diether molecul ... | 1990 | 2223853 |
| [a method for finding the minimal treponemicidal concentration of penicillin]. | 1990 | 2256392 | |
| [efficient hygiene precautions in the household today]. | the results of epidemiological investigations justifies the assumption that increasing health defects, especially enteritis infectiosa, are caused inter alia by inadequate hygienic conditions in households. the number of such diseases ranges between 100,000 and more than 1,000,000 cases per year in the frg. responsible for this development is a lack of information about the behaviour of microorganisms in the environment and its pathways of distribution. in addition risks are growing with the rec ... | 1989 | 2500806 |
| [the aerobic air microflora in airplanes on various international routes]. | aerobic microflora (bacteria, fungi), in the cock pits of the tarom company (boeing 707 and il 62 m) airships flying on various international routes and airports was studied during november 1988-january 1989. 157-8,800 bacteria and 78-1,336 fungi per m3 air were recorded. except for staphylococcus aureus (hemolytic and non hemolytic) the greatest part of the isolated microorganisms was nonpathogenic for man: bacillus, corynebacterium, neisseria, staphylococcus epidermidis, sarcina, aspergillus, ... | 1989 | 2616999 |
| comparison of killing of gram-negative and gram-positive bacteria by pure singlet oxygen. | gram-negative and gram-positive bacteria were found to display different sensitivities to pure singlet oxygen generated outside of cells. killing curves for salmonella typhimurium and escherichia coli strains were indicative of multihit killing, whereas curves for sarcina lutea, staphylococcus aureus, streptococcus lactis, and streptococcus faecalis exhibited single-hit kinetics. the s. typhimurium deep rough strain ta1975, which lacks nearly all of the cell wall lipopolysaccharide coat and mani ... | 1989 | 2703469 |
| influence of ph extremes on sporulation and ultrastructure of sarcina ventriculi. | distinct morphological changes in the ultrastructure of sarcina ventriculi were observed when cells were grown in medium of constant composition at ph extremes of 3.0 and 8.0. transmission electron microscopy revealed that at low ph (less than or equal to 3.0) the cells formed regular packets and cell division was uniform. when the ph was increased (to greater than or equal to 7.0), the cells became larger and cell division resulted in irregular cells that varied in shape and size. sporulation o ... | 1989 | 2738022 |
| antimicrobial activity of julifloricine isolated from prosopis juliflora. | antimicrobial activity of julifloricine, an alkaloid isolated from prosopis juliflora, was studied in vitro against 40 microorganisms which included 31 bacteria, two candida species, five dermatophytic fungi and two viruses. significant inhibitory effect was noted against gram positive bacteria. the minimal inhibitory concentration (mic) for staphylococcus aureus, s. epidermidis, s. citreus, streptococcus pyogenes and sarcina lutea was 1 microgram/ml and against s. faecalis, s. pneumoniae, s. la ... | 1989 | 2775330 |
| [the distribution of microorganisms in household kitchens. i. problems, experiments, results]. | epidemiological investigations have demonstrated that insufficient hygiene in households results in increasing health hazards. in order to be able to recommend ways to disrupt infection chains it was necessary to explore the most important pathways of cross-contamination. housewives without special training were instructed to prepare a complete meal, in the kitchen of a modern, vacant apartment. among the raw products provided was minced meat, contaminated with sarcinae (unbeknown to the housewi ... | 1988 | 3134770 |
| [the distribution of microorganisms in household kitchens. ii. evaluation of results and hygienic inferences]. | the very high morbidity rates of enteritis infectiosa diseases demand improved prophylactic measures. an important indication of the source of these illnesses is the fact that infections in private households are about three times more frequent than in canteens. indeed, the rise in morbidity is undoubtedly caused by inadequate treatment of raw products, of meal rests and by insufficient heating processes. furthermore, in household kitchens no efforts are made to interrupt infection chains, and d ... | 1988 | 3134771 |
| ecophysiological adaptations of anaerobic bacteria to low ph: analysis of anaerobic digestion in acidic bog sediments. | the dynamics of anaerobic digestion were examined in the low-ph sediments of crystal bog in wisconsin. the sediments (ph 4.9) contained 71% organic matter and the following concentrations of dissolved gases (micromoles per liter): co2, 1,140; ch4, 490; and h2, 0.01. the rate of methane production was 6.2 mumol/liter of sediment per h, which is slower than eutrophic, neutral sediments. microbial metabolic processes displayed the following ph optima: hydrolysis reactions, between 4.2 and 5.6; acet ... | 1987 | 3103534 |
| factors affecting the in-vitro activity of roxithromycin. | the effect of human serum and co2 on the activity of roxithromycin and erythromycin was assessed. protein binding of roxithromycin in serum from various animal sources, acid alpha 1-glycoprotein and human albumin v was determined. there was a four- to eight-fold increase in mic and mbc of roxithromycin in the presence of 70 and 100% human serum (minimum effect seen with erythromycin) and for both compounds there was a four- to eight-fold increase in mic for fastidious organisms in the presence o ... | 1987 | 3123452 |
| physiological adaptations of anaerobic bacteria to low ph: metabolic control of proton motive force in sarcina ventriculi. | detailed physiological studies were done to compare the influence of environmental ph and fermentation end product formation on metabolism, growth, and proton motive force in sarcina ventriculi. the kinetics of end product formation during glucose fermentation in unbuffered batch cultures shifted from hydrogen-acetate production to ethanol production as the medium ph dropped from 7.0 to 3.3. at a constant ph of 3.0, the production of acetate ceased when the accumulation of acetate in the medium ... | 1987 | 3571164 |
| bioequivalence studies of five brands of ampicillin trihydrate capsules. | using the microbiological assay method, the bioavailability of ampicillin in healthy human volunteers has been examined. the plasma levels were used to determine the extent and rate of bioavailability following oral administration of 250 mg capsules produced by 5 different manufacturers. balanced incomplete block design was employed and standard statistical techniques were used to detect any significant difference between multivariate and univariate characteristics. the results indicate that the ... | 1987 | 3592855 |
| food-borne amines and amides as potential precursors of endogenous carcinogens. | this paper reviews the experimental results of our research in the past several years and other related papers that have been directed toward the occurrence, biotransformation and epidemiological significance of carcinogenic n-nitroso compounds in biosphere. endogenous carcinogens are a group of cancer-causing compounds produced in vivo from harmless precursors. this category has been exemplified by the well-known carcinogens, n-nitroso compounds. the significance of naturally occurring amines a ... | 1986 | 3714904 |
| pharmacokinetics of cefotaxime, moxalactam, and cefoperazone in the early puerperium. | twelve parturient women volunteered to receive 1 g of cefotaxime on the second or third day postpartum by intravenous infusion over 3 min. blood was taken from the antecubital vein of the contralateral arm at 0.25, 0.5, 0.75, 1, 2, 4, and 6 h. the concentration of cefotaxime in serum was assayed by agar diffusion, with sarcina lutea atcc 9341 as the indicator strain. the same 12 women received an identical dose of antibiotic 4 months after the first dose, and blood was taken at the same time int ... | 1986 | 3729345 |
| roxithromycin bioassay procedures for human plasma, urine and milk specimens [corrected and issued with original paging in eur j clin microbiol 1986 dec;5(6)]. | 1986 | 3780710 | |
| plasma penicillin-g parameters in man after intraduodenal or intramuscular administration. | concentrations of penicillin-g produced in serum by either direct intraduodenal (i.d.) or intramuscular (i.m.) administration were compared in 9 healthy volunteers. serum specimens were assayed by the sarcina lutea cup plate method. the peak serum levels achieved by i.d. application were similar to those observed after intramuscular dose. however, higher concentrations were better sustained after i.m. penicillin. we concluded that intraduodenal administration of penicillin-g could be considered ... | 1986 | 3793296 |
| [isolation and study of the biological properties of substances stimulating levorin biosynthesis in a mixed actinomycete and yeast culture]. | active components responsible for increased synthesis levels of levorin, a polyenic antibiotic in mixed cultures of an actinomycete and a yeast-like fungus c. tropicalis were isolated from the fermentation broth. component i identified as succinic acid increased the levels of the antibiotic production by 40-50 per cent. component ii found to be lactic acid increased the antibiotic production levels by 20 per cent. component iii, a mixture of nonidentified substances of the amine nature stimulate ... | 1986 | 3545067 |
| bacterial growth rates and competition affect nodulation and root colonization by rhizobium meliloti. | the addition of streptomycin to nonsterile soil suppressed the numbers of bacterial cells in the rhizosphere of alfalfa (medicago sativa l.) for several days, resulted in the enhanced growth of a streptomycin-resistant strain of rhizobium meliloti, and increased the numbers of nodules on the alfalfa roots. a bacterial mixture inoculated into sterile soil inhibited the colonization of alfalfa roots by r. meliloti, caused a diminution in the number of nodules, and reduced plant growth. enterobacte ... | 1986 | 16347173 |
| nutrition and growth characteristics of trichomitopsis termopsidis, a cellulolytic protozoan from termites. | putatively axenic cultures of trichomitopsis termopsidis 6057, isolated by m. a. yamin (j. protozool. 25:535-538, 1978) from the hindgut of zootermopsis termites, apparently contained methanogenic bacteria, inasmuch as small amounts of ch(4) were produced during growth. however, t. termopsidis could be "cured" of methanogenic activity by incubation in the presence of bromoethanesulfonate. both the cured derivative (6057c) and the parent strain (6057) required nahco(3) and fetal bovine serum for ... | 1985 | 16346754 |
| cefoxitin and cefamandole levels in the human bile. | the bile levels of cefoxitin and cefamandole were studied in 75 patients. the two antibiotics were shown to be present at high concentration and to undergo a rapid metabolization. it is concluded that they represent the antibiotics of choice in the prophylactic and therapeutic treatment of biliary tract infections. | 1985 | 3830955 |
| pharmacokinetic studies of azlocillin and piperacillin during late pregnancy. | in this paper pharmacokinetic data of acylureidopenicillins (azlocillin and piperacillin) in pregnant women in the last trimenon are compared with those from nonpregnant controls. four g of each drug was administered as slow bolus injection intravenously. the concentrations in serum and urine of both drugs were determined by polarographic and microbiological methods, for comparison. we used for evaluation of pharmacokinetic data a computer program based on the equations of a two-compartment open ... | 1985 | 3908007 |
| pharmacokinetic study of a paediatric formulation of amoxycillin and clavulanic acid in children. | a combination of amoxycillin and clavulanic acid 4:1 was administered to 35 children (aged 2 to 10 years) with infections. the combination was administered orally as a suspension, every 8 h for 5 to 7 days. sixteen children (aged 2 to 5 years), received 125 mg amoxycillin and 31.25 mg clavulanic acid, and 19 (6 to 10 years) received 250 mg amoxycillin and 62.5 mg clavulanic acid per dose. following the first dose serum concentrations of amoxycillin and clavulanic acid were determined by microbio ... | 1985 | 3908124 |
| comparison of liquid chromatographic and bioassay procedures for determining depletion of intramuscularly injected tylosin. | crossbred pigs weighing 80-110 kg were injected intramuscularly in the ham with 8.8 mg/kg tylosin. animals were slaughtered in groups of 3 at intervals of 4 h, and 1, 2, 4, and 8 days after injection, and samples of blood, injected muscle, uninjected muscle, liver, and kidney were analyzed by liquid chromatography (lc) and by bioassay using sarcina lutea as the test organism. the lc method was far more sensitive with a detection limit of less than 0.1 ppm, while the detection limit by bioassay w ... | 1985 | 4019360 |
| a note on the contamination of eye-drops following use by hospital out-patients. | examination of 273 eye-drops, returned to a hospital pharmacy by out-patients showed that 27% were contaminated. common contaminants included bacillus spp, staphylococcus albus and sarcina spp. those eye-drops containing the preservatives chlorbutol or thiomersal showed above average levels of contamination, thus questioning the suitability of these preservatives for ophthalmic preparations. | 1985 | 4019793 |
| [antimicrobial activity of some medicinal species from the dakar markets]. | after drawing up an inventory of dakar markets popular medicinals plants, antimicrobial activity of 43 species have been screened. with the agar diffusion method, crude extracts of 24 species show an activity against sarcina lutea or escherichia coli. the antimicrobial spectrum of these extracts against 11 germs has been evaluated with the agar dilution method. 13 species show m.i.c. lower than 10 mg/ml against some germs. this preliminary study select some plants of potential interest for devel ... | 1985 | 4048256 |
| connections between ascaridia galli and the bacterial flora in the intestine of hens. | parasitological dissections of 502 intestinal tracts of hens deriving from big private chicken-farms have been done. in the jejunum of 146 hosts (ext. 29.1%) from 1 to 21 individuals of a. galli were detected. using bacterial selective media and biochemical tests, the microorganisms from the hen's intestinal tracts as well as from the cuticle surface of the nematodes were identified. among them were: grampositive (+) lactobacillus, bacillus, staphylococcus, streptococcus, micrococcus, sarcina, c ... | 1985 | 4061960 |
| [quantitative microbiologic determination of organophosphorus compounds]. | 1985 | 4091983 | |
| the rate of evolutionary divergence of initiation factors if2 and if3 in various bacterial species determined quantitatively by immunoblotting. | antibodies to escherichia coli translational initiation factors if2 and if3 were used for an immunological comparison of unpurified proteins from the following genera: salmonella, serratia, proteus, aeromonas, pseudomonas, streptococcus, sarcina and bacillus. immunological relatedness was compared by ouchterlony double diffusion experiments and immunoblotting analysis. immunoblotting is a quantitative technique for measuring levels of specific proteins in crude cell lysates. we have used this te ... | 1984 | 6084987 |
| production of a macromomycin (mcr)-monoclonal antibody conjugate and its biological activity. | macromomycin (mcr), an unique membrane-reactive anticancer antibiotic, was incubated with murine monoclonal anti-hla igg1 antibody (h-1) in the presence of carbodiimide. the resulting mixture was fractionated with a sephadex g-200 column. the first and second fractions were shown to contain mcr-(h-1) conjugate by the elution profile, as well as by the sarcina lutea growth inhibition assay and ouchterlony double-diffusion method. a membrane immunofluorescence test with anti-mcr and anti-mouse igg ... | 1984 | 6375268 |
| comparison of bone levels after intramuscular administration of cephradine ('velosef') or flucloxacillin/ampicillin in hip replacement. | serum and bone levels of antibiotics were measured after the intramuscular injection of either 1 g cephradine or 500 mg flucloxacillin plus 500 mg ampicillin in 48 patients undergoing total hip replacement. the results showed that both regimens produced adequate blood levels, but that the levels after cephradine were higher and more prolonged. bone levels of cephradine were significantly higher than for flucloxacillin or ampicillin. in half of the patients studied, flucloxacillin bone levels wer ... | 1984 | 6463064 |
| penicillin compliance in splenectomized thalassemics. | compliance with penicillin prophylaxis after splenectomy was studied in 42 thalassemic children and adolescents. urine samples were tested five times per patient for the presence of penicillin by the sarcina lutea inhibition test. only 7% of patients were found to be absolute noncompliers, while an additional 14% had a compliance considered to be insufficient. noncompliers tended to have been splenectomized more than 5 years before, while couples of affected siblings were uniformly full-complier ... | 1984 | 6468437 |
| [fractionation of carbon isotopes by a methane-forming sarcina]. | 1984 | 6479008 | |
| contamination of irrigation solutions in an operating theatre. | sterile irrigation solution, used in open systems in a conventional orthopedic operating theatre, were examined for bacterial contamination. at the end of 13 of 21 operations (duration greater than or equal to one hour) the solution tested displayed the growth of staphylococcus epidermidis and/or diphtheroid rods and/or other species. it is concluded that irrigation by solutions, kept in open bowls during an operation, is a potential source of contamination. | 1984 | 6564086 |
| n-nitrosophenacetin: its synthesis, characterization, mutagenicity, and teratogenicity. | reaction of phenacetin (cas: 62-44-2; p-acetophenetidide) with nitrous fumes (n2o3) in glacial acetic acid at 0-5 degrees c yields n-nitrosophenacetin (np), 2-nitrophenacetin, n-nitroso-2-nitrophenacetin (nnp), and other compounds. both np and nnp are fairly stable at low temperature (-30 degrees c) but extremely labile at ambient temperature. np (median lethal dose to sprague-dawley rat: 21 mg/kg body wt) is 80 times more toxic than its parent compound phenacetin and is directly mutagenic to ba ... | 1984 | 6584662 |
| salt and base sequence specific changes in the chiroptical properties of dna. | chiroptical properties of natural dna molecules differing in base composition were studied in solutions with high concentrations of monovalent sodium and caesium salts. it was found that the properties were dependent on the dna base sequence and nature of both cations and anions. a comparison with the behaviour of the synthetic molecules of dna demonstrated that the salt-induced changes in the natural molecules of dna could not be accounted for by the appearance of the left-handed z conformation ... | 1984 | 6434373 |
| [effect of the methods of levamisole administration on selected elements of cellular immunity in guinea pigs]. | 1984 | 6443815 | |
| a note on the temperature tolerance of legionella. | a strain of legionella pneumophila serogroup 1 isolated from the environment had a decimal reduction time in water at 50 degrees c (d50) of 111 min, a d54 of 27 min and a d58 of 6 min. there was little loss of viability at 46 degrees c. other environmental organisms, a pseudomonas sp., a micrococcus sp. and a coliform survived less well at these temperatures. a species of sarcina had a survival time greater than the l. pneumophila at all the temperatures tested. other strains of legionellas were ... | 1984 | 6725165 |
| [antimicrobial activity in tissues of swine following subcutaneous administration of turimycin]. | 1983 | 6651444 | |
| antibacterial activity of wratizolin. | wratizolin in the concentration of 1-10 micrograms/ml was found to inhibit the growth of gram-positive bacteria such as: staphylococcus, streptococcus, sarcina, corynebacterium, and some representatives of aerobic bacilli. gram-negative bacteria of enterobacteriaceae and pseudomonas were not sensitive to wratizolin even in the concentration of 100 micrograms/ml. | 1983 | 6675569 |
| molecular properties of succinate dehydrogenase isolated from micrococcus luteus (lysodeikticus). | succinate dehydrogenase (ec 1.3.99.1) of micrococcus luteus was selectively precipitated from triton x-100-solubilized membranes by using specific antiserum. the precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. the 72,000 polypeptide possessed a covalently bound flavin prosthetic group and appeared to be strongly antigenic as judged by immunoprinting experiments. low-temperature absorption spectroscopy rev ... | 1983 | 6402500 |
| determination of penicillin g, ampicillin, and cephapirin residues in tissues. | a cylinder plate microbiological method was developed for the rapid, quantitative determination of penicillin g, ampicillin, and cephapirin in animal tissues. the method uses agar plates seeded with stable spores of bacillus stearothermophilus var. calidolactis and incubated 4 h at 64 degrees c. standard curves were obtained for the following ranges of concentration of antibiotics in tissues: 0.02-0.32 iu penicillin g/g, 0.0125-0.2 micrograms ampicillin/g, and 0.02-0.32 micrograms cephapirin/g. ... | 1983 | 6826504 |
| survey, purification, and properties of sugar phosphate phosphohydrolase among microorganisms. | sugar phosphate phosphohydrolase was purified approximately 500- to 600-fold to apparent homogeneity from escherichia coli b, escherichia coli c, escherichia coli var. communior, escherichia acidilactici, enterobacter aerogenes, neisseria meningitidis, and saccharomyces cereviseae. the molecular weights of the enzyme as estimated by gel filtration ranged from 97 x 10(3) to 101 x 10(3). the enzyme was composed of two subunits with the same molecular weight which ranged from 50 x 10(3) to 52 x 10( ... | 1983 | 6322944 |
| methanogenic bacteria from the bondyuzhskoe oil field: general characterization and analysis of stable-carbon isotopic fractionation. | selective enrichment culture techniques were employed to obtain mixed cultures of methanogenic rods and sarcina from surface flooding waters and deep subsurface ( approximately 1650 m) oil-bearing sedimentary rocks and formation waters sampled from an old oil field in the u.s.s.r. previously reported to display active biological methanogenesis. the methanogens were selectively isolated as colonies on agar petri dishes that were incubated in a novel container. the general cellular and growth feat ... | 1983 | 16346216 |
| l-serine production by a mutant of sarcina albida defective in l-serine degradation. | for improved l-serine production, an l-serine dehydratase-defective mutant of sarcina albida iam 1012 was obtained. in the mutant, the activities of the enzymes responsible for l-serine production were as high as those in the parent strain, and, at a low glycine concentration, the mutant accumulated l-serine more efficiently than the parent. under optimum conditions, 21 mg of l-serine per ml accumulated from 100 mg of glycine per ml. l-serine was isolated from a reaction mixture as l-serine m-xy ... | 1983 | 16346305 |
| culture medium for confirmation of penicillin-resistant and penicillinase-producing neisseria gonorrhoeae. | a culture method for the isolation and identification of penicillinase (beta-lactamase)-producing neisseria gonorrhoeae (ppng) was evaluated in the philippines where ppng are common. the method uses plastic biplates containing standard martin-lewis gonorrhea culture medium in one side of the biplate and ppng-selective medium containing 1.5 microgram of penicillin g per ml and a suspension of sarcina lutea (micrococcus lutea) that was susceptible to 0.01 microgram of penicillin g per ml in the ot ... | 1982 | 6808016 |
| antimicrobial characteristic of insoluble alkylpyridinium iodide. | insoluble and soluble alkylpyridinium iodides (c8 to c18) were synthesized. the insoluble agents were quaternized 4-vinylpyridine-divinylbenzene copolymers. the insoluble agent [c12(50)] that contained 50% divinylbenzene and had a c12 alkyl chain was selected as the most suitable insoluble agent. c12(50) showed poor durability of the antibacterial activity, but c12(50), which had lost the activity, was refreshed by washing with ethanol. this washing became ineffective after a few cycles of antib ... | 1982 | 6808918 |
| determination of apalcillin and its metabolites in human body fluids by high-pressure liquid chromatography. | we describe two methods for the quantitative analysis of apalcillin and its metabolites in serum and urine by reverse-phase high-pressure liquid chromatography (hplc), a fast isocratic method for the parent drug, and a gradient method that allows the simultaneous assay of two metabolites. serum was deproteinized with acetonitrile, and urine was diluted with buffer solution. the detection limit was about 0.5 micrograms/ml at a detection wavelength of 254 nm and 1.5 micrograms/ml at 310 nm. within ... | 1982 | 6818901 |
| [kinetic and clinical studies on a new cephalosporin: cefotiam]. | kinetic and clinical evaluation of cefotiam, a new cephalosporin, is reported. it was found that the drug is rapidly distributed to the tissues. equilibrium between tissues and plasma is reached in about an hour. some 90-91% of the dose administered is excreted in the urine, and accumulation does not occur. a clinical cure was obtained in 27 of a series of 35 patients (77.1%). improvement was observed in 7 cases (20%). the antibiotic proved ineffective in the remaining cases (2.8%). tolerance wa ... | 1982 | 6289193 |
| largomycin: preparation, properties, and structure. | largomycin has been purified to homogeneity by chromatography on hydroxylapatite whereby carbohydrate and protease impurities were removed. largomycin is an acidic protein (pi 4.13, molecular weight 29300) which forms a dimer in phosphate buffer. an n-terminal amino acid sequence analysis from the amino-terminal residue gave, for the first 32 residues, asp-ile-leu-ile-ala-gly-ala-thr-gly-asn-val-gly-lys-pro-leu-val-glu-gly-leu-leu - ala-ala-gly-lys-pro-val-arg-ala-leu-thr-arg-asn... the sequence ... | 1982 | 7138848 |
| oral penicillin prophylaxis in children with imparied splenic function: a study of compliance. | one measure used to prevent overwhelming sepsis due to streptococcus pneumoniae in children with defective splenic function is oral penicillin prophylaxis. however, a frequently cited argument against this approach is the likelihood of poor compliance. compliance was studied by examining urine specimens for penicillin by the sarcina lutea disc diffusion technique in 22 surgically asplenic children, two patients following bone marrow transplantation, and 38 infants and young children with sickle ... | 1982 | 7145549 |
| correlation between fluorimetric and microbiological methods for determination of cephalexin in urine and serum. | the fluorimetric and microbiological methods for the determination of serum and urine cephalexin were compared with the aid of coded samples in two different laboratories. the coefficient of correlation between the two methods for serum cephalexin was 0.94. at urine cephalexin concentrations above 0.5 g/l, the coefficient of correlations between two methods was 0.83. at lower concentrations (0.5 g/l), the coefficient of correlation was 0.72. it was concluded that the tested methods are comparabl ... | 1981 | 7205158 |
| [kinetic and clinical study of bacampicillin]. | a mean plasma concentration curve, with peaks within the first hour and moderate individual variations, was obtained after a single administration of 800 mg of bacampicillin to 5 healthy volunteers. an adequate antibiotic transfer from the circulation to the bronchial apparatus was demonstrated. treatment of 34 patients suffering from ampicillin-sensitive bacterial diseases permitted clinical cure of all patients, without onset of side-effects. it is concluded that bacampicillin is preferable to ... | 1981 | 7219802 |
| the effect of temperature on the growth and lipid composition of the extremely halophilic coccus, sarcina marina. | sarcina marina (ncmb 778) grew over the temperature range 20-45 degrees c but no growth was recorded at 15 degrees c or 50 degrees c. at the optimum growth temperature of 34 degrees c the doubling time was 14.5 h. the major polar lipid components, tentatively identified as the diether analogues of phosphatidyl glycerophosphate (pgp), phosphatidyl glycerol (pg), diglycosyl diglyceride (dgd) and triglycosyl diglyceride (tgd), and the major neutral lipid components, tentatively identified as squale ... | 1981 | 7247392 |
| fluorimetric and microbiological assays of erythromycin concentrations in plasma and vaginal washings. | using a microbiological and a fluorimetric assay to determine penetration of erythromycin into vaginal fluids, concentrations were measured in plasma from nine men one hour after a single oral dose of erythromycin stearate 2 g and in vaginal washings and plasma samples taken simultaneously from 11 women two hours after the last dose of a 10-day course of erythromycin stearate (250 mg four times daily). both assay methods gave accurate and reproducible results in plasma but only the fluorimetric ... | 1981 | 7272705 |
| photosensitivity in carotenoidless mutants of micrococcus luteus (sarcina lutea): non-photodynamic menaquinone destruction in white light. | 1981 | 7280045 | |
| pharmacokinetics of cefamandole in osseous tissue. | the ability of cefamandole to cross osseous capillary membranes and its concentrations in interstitial fluid spaces are studied in canine cortical bone. extraction studies were performed by using indicator dilution techniques and demonstrated that cefamandole readily traversed osseous capillary membranes. volume-of-distribution studies demonstrated that cefamandole was distributed in the plasma and interstitial fluid spaces of cortical bone. there was a direct correlation between the calculated ... | 1981 | 7294769 |
| influence of curvature of response lines in antibiotic agar diffusion assays. | 1981 | 6783664 | |
| a simple method for the determination of dna molecular weight distribution. | a method is described for the molecular weight distribution of dna which is determined from sedimentation-velocity analysis. knowing the distribution of sedimentation coefficients for a single dna concentration it is possible to extrapolate such a distribution to infinite dilution of the solute in a simple way. two versions (using two or three terms of a series) of extrapolating equations are considered and discussed in detail. the sedimentation coefficients distribution calculated from these eq ... | 1981 | 6785340 |
| the second international standard for erythromycin. | 1981 | 6792202 | |
| [recovery of germs on surfaces with the collector by thran (author's transl)]. | 1981 | 6798780 | |
| [recovery of germs dried on surfaces with the collector by thran]. | 1981 | 6798781 | |
| bioassay of clindamycin in bile. | this study compares different preparations of standard solutions for determining clindamycin in bile. pooled human serum, ox bile, 10% human bile, and 0.1 m phosphate buffer were compared, and pooled human bile was serving as reference standard curve. the use of pooled human serum and ox bile resulted in a considerable error of overestimation of clindamycin in bile. an error of underestimation occurred when 10% human bile or 0.1 m phosphate buffer was used as a standard solution. it is concluded ... | 1981 | 7471906 |
| bioassay of antibiotics in body fluids from patients receiving cancer chemotherapeutic agents. | patients receiving antitumor chemotherapy are at increased risk of developing nosocomial infections, and the antibacterial therapy of such infections is often monitored by bioassay. the effect of antitumor agents on seven bioassay procedures using strains of sarcina, klebsiella, clostridium, pseudomonas, staphylococcus aureus, and s. epidermidis or bacillus was evaluated. the minimum inhibitory concentrations of six antitumor drugs, cytarabine, dactinomycin, doxorubicin, 5-fluorouracil, methotre ... | 1980 | 6903435 |
| simple and microbiologically safe portable transport unit for patients requiring protected isolation. | the removal of a patient from a laminar air flow room during protected isolation poses the risk of his microbial contamination and infection. nonetheless, temporary removal from the laminar air flow room may be necessary for certain medical procedures or for psychological relief during prolonged periods of isolation or both. a means for protecting patients during temporary removal from the laminar air flow room is highly desirable. a previously described total body garment has been shown to be m ... | 1980 | 6769945 |
| [airborne microflora found in some stations of the metro in the hungarian capital of budapest (author's transl)]. | both the microflora and some physical parameters of the air in three underground railway (metro) stations were analysed. the air samples taken in the first two stations contained considerably more microbes, namely three times as many, than the sample from station no. 3. in the samples from the first two stations both the pathogenic and anthropogenic germs occured more frequently than in the samples from the third station. the normal flora consisted of gram-positive cocci/streptococci, micrococci ... | 1980 | 6775455 |
| lymphotropic accumulation of an antitumor antibiotic protein, neocarzinostatin. | 1980 | 6446456 | |
| fast and simple method for the separation of intermediates and cofactors involved in the biosynthesis of cephalosporin c using chemically bonded c12 reversed-phase thin-layer chromatography. | 1980 | 7380971 | |
| purification and characterization of macromomycin-i, a protein antibiotic containing a non-protein chromophore. | 1980 | 7396932 | |
| [preservation of lyophilized cultures of saprophytic microorganisms]. | all of the tested saprophytic microbial cultures remained viable upon 11--16 years of storage in the lyophilized state at 3--6 degrees. the cultures belonged to the following taxonomic groups: acetobacter, achromobacter, azotobacter, bacillus, chromatium, escherichia, flavobacterium, lactobacillus, micrococcus, proteus, propionibacterium, pseudomonas, rhizobium, sarcina, serratia, streptococcus, torula, mycobacterium, actinomyces. the number of viable cells in the tubes decredased only slightly ... | 1980 | 7412623 |
| the microbial transformation of tylosin by the spiramycin-producing strain, streptomyces ambofaciens ka-1028. | 1980 | 7429995 |