Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| community dynamics of bacteria in sourdough fermentations as revealed by their metatranscriptome. | the lactic acid bacterial (lab) community dynamics of two wheat and two spelt sourdough fermentations that were daily back-slopped were monitored during a period of 10 days by hybridizing time-related rna samples, representing the metatranscriptome, to an lab functional gene microarray. to indicate the species present in each hybridized sample, annotation information for the 2,269 oligonucleotides on the microarray was used. the overall hybridization data revealed that after a transition phase o ... | 2010 | 20581179 |
| diversity of lactic acid bacteria from hussuwa, a traditional african fermented sorghum food. | the diversity of lactic acid bacteria associated with hussuwa fermentation, a sudanese fermented sorghum food, was studied using a polyphasic taxonomical approach. predominant strains could be well characterised based on a combination of phenotypic tests and genotypic methods such as ardra, rep-pcr and rapd-pcr, as well as 16s rrna gene sequencing of representative strains. thus, the majority (128 of 220, 58.3%) of strains exhibited phenotypic properties typical of heterofermentative lactobacill ... | 2010 | 20630317 |
| effects of microbial inoculants on corn silage fermentation, microbial contents, aerobic stability, and milk production under field conditions. | the present study aimed to investigate the effects of 2 corn silage inoculation strategies (homofermentative vs. heterofermentative inoculation) under field conditions and to monitor responses in silage variables over the feeding season from january to august. thirty-nine commercial dairy farms participated in the study. farms were randomly assigned to 1 of 3 treatments: control (nonactive carrier; chr. hansen a/s, hørsholm, denmark), lactisil (inoculation with 1 x 10(5)lactobacillus pentosus an ... | 2010 | 20655446 |
| microorganisms associated with maari, a baobab seed fermented product. | a microbiological study was carried out on baobab fermented seeds (maari) obtained from 4 different production sites in burkina faso (mansila, toulfé, ouagadougou and gorgadji). a total of 390 representative isolates comprising 251 aerobic mesophilic bacteria (amb) and 139 lactic acid bacteria (lab) were isolated and identified to species level using a combination of pheno- and genotypic methods including conventional morphological analysis, carbohydrate fermentation profiling, rep-pcr ((gtg)(5) ... | 2010 | 20688411 |
| a real-time pcr assay for detection and quantification of 2-branched (1,3)-beta-d-glucan producing lactic acid bacteria in cider. | ropiness in natural cider is a relatively frequent alteration, mainly found after bottling, leading to consumer rejection. it is derived from the production of exopolysaccharides (eps) by some lactic acid bacteria most of which synthesize a 2-branched (1,3)-beta-d-glucan and belong to the genera pediococcus, lactobacillus and oenococcus. this polysaccharide synthesis is controlled by a single transmembrane glycosyltransferase (gtf). in this work, a method based on quantitative pcr (qpcr) and tar ... | 2010 | 20696488 |
| tyrosine- and histidine-decarboxylase positive lactic acid bacteria and enterococci in dry fermented sausages. | lactic acid bacteria (lab) and enterococci were isolated immediately after stuffing (day 0), at the end of ripening (28th day) and at the end of storage (112th day) from dry fermented sausages produced by two different producers (k; r) in two diameters (4.5 and 7 cm) using either of two spice mixtures (p; h) and either of two starter cultures (pediococcus pentosaceus, c; lactobacillus curvatus+staphylococcus carnosus, f), resulting in a total of 16 different combinations. tyrosine-decarboxylase ... | 2010 | 20696534 |
| heterologous production of pediocin pa-1 in lactobacillus reuteri. | the recombinant dna plr5cat_psab in which pediocin pa-1 structural and immunity genes (pedab) fused with the promoter and deduced signal sequence of an alpha-amylase gene from a bifidobacterial strain were inserted in plr5cat, an escherichia coli-lactobacilli shuttle vector was transferred to lactobacillus reuteri kctc 3679 and the transformant presented bacteriocin activity. the recombinant l. reuteri kctc 3679 transformed with the shortened plr5cat(s)_psab, where non-essential region for the l ... | 2010 | 20798585 |
| exploitation of acha (digitaria exiliis) and iburu (digitaria iburua) flours: chemical characterization and their use for sourdough fermentation. | this study aimed at characterizing the chemical and microbiological composition of acha (digitaria exiliis) and iburu (digitaria iburua) flours and at exploiting their potential for sourdough fermentation. both the flours had a gross composition similar to other cereals. as shown by two-dimensional electrophoresis analysis, acha flour had a higher number of prolamins with respect to iburu flour. the opposite was found for the number of glutelin spots. the concentration of total free amino acids ... | 2010 | 20832683 |
| differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers. | obesity alters gut microbiota ecology and associates with low-grade inflammation in humans. roux-en-y gastric bypass (rygb) surgery is one of the most efficient procedures for the treatment of morbid obesity resulting in drastic weight loss and improvement of metabolic and inflammatory status. we analyzed the impact of rygb on the modifications of gut microbiota and examined links with adaptations associated with this procedure. | 2010 | 20876719 |
| new nanostructured electrochemical biosensors based on three-dimensional (3-mercaptopropyl)-trimethoxysilane network. | the design and characterization of a new nanostructured organic-inorganic hybrid material and its application to l-lactic acid determination are described. this material is based on the integration of the enzyme lactate oxidase (lox) and gold nanoparticles (aunps) into a sol-gel 3d polymeric network derived from (3-mercaptopropyl)-trimethoxysilane (mpts) previously formed onto a gold surface. mpts presents the advantage of forming a 3d polymeric network containing a large number of thiol tail gr ... | 2010 | 21072332 |
| the efficacy of lactic acid bacteria pediococcus acidilactici, lactose and formic acid as dietary supplements for turkeys. | a feeding trial was performed on 1400 big-6 turkey toms divided into experimental groups subject to the use of dietary supplements. the ain of this study was to evaluate the efficacy of the probiotic supplement bactocell, containing lactic acid bacteria pediococcus acidilactici, and lactose, administered to turkeys separately or in combination, as well as a formic acid supplement. the addition of the probiotic under test (lactic acid bacteria pediococcus acidilactici) to diets for turkeys contri ... | 2010 | 21077430 |
| species diversity and relative abundance of lactic acid bacteria in the milk of rhesus monkeys (macaca mulatta). | mother's milk is a source of bacteria that influences the development of the infant commensal gut microbiota. to date, the species diversity and relative abundance of lactic acid bacteria in the milk of non-human primates have not been described. | 2010 | 20946146 |
| identification of escherichia coli o157:h7 surrogate organisms to evaluate beef carcass intervention treatment efficacy. | we compared the survival of potential pathogen surrogates-meat-hygiene indicators (non-escherichia coli coliforms), biotype i e. coli, and lactic acid bacteria starter cultures-with survival of an e. coli o157:h7 (eco157:h7) inoculum in beef carcass intervention trials. survival of one lactic acid bacteria starter culture (bactoferm lhp dry [pediococcus acidilactici and pediococcus pentosaceus]), a five-isolate biotype i inoculum, and a five-isolate non-e. coli coliform inoculum, was compared wi ... | 2010 | 21067675 |
| sco3900, co-transcripted with three downstream genes, is involved in the differentiation of streptomyces coelicolor. | sco3900, a putative target gene of the developmental regulator whii of streptomyces coelicolor, encodes a protein similar to padr from pediococcus pentosaceus. the sco3900 disruption mutant exhibited a bald phenotype with sparse aerial hyphae. however, a single copy of sco3900 under the control of its native promoter could not complement the mutant phenotype. a fragment containing sco3900 and its downstream three genes completely restored the mutant phenotype to the wild-type. these suggested th ... | 2010 | 20012957 |
| effect of probiotic pediococcus acidilactici on antioxidant defences and oxidative stress of litopenaeus stylirostris under vibrio nigripulchritudo challenge. | antioxidant defences and induced oxidative stress tissue damage of the blue shrimp litopenaeus stylirostris, under challenge with vibrio nigripulchritudo, were investigated for a 72-h period. for this purpose, l. stylirostris were first infected by immersion with pathogenic v. nigripulchritudo strain sfn1 and then antioxidant defences: superoxide dismutase (sod), catalase (cat), glutathione peroxidase (gpx), total antioxidant status (tas), glutathiones and induced tissue damage (mda and carbonyl ... | 2010 | 20045735 |
| potential of pediococcus pentosaceus (l006) isolated from maize leaf to suppress fumonisin-producing fungal growth. | the present study was aimed at characterizing the ability of lactic acid bacteria isolated from maize to repress the growth of fumonisin-producing fungi. a total of 67 isolates were screened for their antifungal activity against fusarium proliferatum and fusarium verticillioides by using the overlay method. the most efficient antifungal isolate was identified as pediococcus pentosaceus (l006), on the basis of physiological and biochemical characterization and 16s rrna gene sequencing. production ... | 2010 | 20537273 |
| efficacy of lactic acid bacteria (lab) supplement in management of constipation among nursing home residents. | constipation is a significant problem in the elderly, specifically nursing home and/or extended-care facility residents are reported to suffer from constipation. lactic acid bacteria (lab) are beneficial probiotic organisms that contribute to improved nutrition, microbial balance, and immuno-enhancement of the intestinal tract, as well as diarrhea and constipation effect. the objective of this study was to investigate the efficacy of this lab supplement in the management of nursing home resident ... | 2010 | 20137076 |
| increased inactivation of exopolysaccharide-producing pediococcus parvulus in apple juice by combined treatment with enterocin as-48 and high-intensity pulsed electric field. | the cyclic peptide bacteriocin enterocin as-48 was tested (at final concentrations of 0.175, 0.613, and 1.05 au/ml) against the exopolysaccharide-producing cider spoilage strain pediococcus parvulus 48 in apple juice in combination with high-intensity pulsed electric field (hipef) treatment (35 kv/cm and 150 hz for 4 mus and bipolar mode). the effect of the combined treatments was studied by surface response methodology, with as-48 concentration and hipef treatment time as process variables. a b ... | 2010 | 20051202 |
| pediococcus pentosaceus sn26 inhibits ige production and the occurrence of ovalbumin-induced allergic diarrhea in mice. | we investigated the anti-allergic effect of a new strain (pediococcus pentosaceus sn26, the sn26 strain) among 59 strains isolated from japanese fermented vegetable pickles, the sunki pickle. the sn26 strain increased th1 type cytokine (il-12 and ifn-gamma) production of peyer's patch (pp) cells in balb/c mice, improved the th1/th2 balance, and inhibited ige production of splenocytes of ovalbumin (ova)-induced allergic diarrheic mice. next we demonstrated, by neutralizing il-12 and ifn-gamma, th ... | 2010 | 20139622 |
| coculture with specific bacteria enhances survival of lactobacillus plantarum nc8, an autoinducer-regulated bacteriocin producer, in olive fermentations. | bacteriocin production in lactobacillus plantarum nc8 is activated by coculture with specific bacteriocin production-inducing bacterial strains. the system is further regulated by a three-component regulatory system involving a specific autoinducer peptide (plnc8if). we have used l. plantarum nc8 as a starter culture in spanish-style green olive fermentations and examined the influence of coculturing in its survival. we found that l. plantarum nc8 greatly enhanced its growth and survival in the ... | 2010 | 20227607 |
| gene cloning, expression, and characterization of phenolic acid decarboxylase from lactobacillus brevis rm84. | phenolic acid decarboxylase (pad) catalyzes the synthesis of vinyl phenols from hydroxycinnamic acids. the gene encoding pad from lactobacillus brevis was cloned and expressed as a fusion protein in escherichia coli. the recombinant pad enzyme is a heat-labile enzyme that functions optimally at 22 degrees c and ph 6.0. the purified enzyme did not show thermostability at temperatures above 22 degrees c. l. brevis pad is able to decarboxylate exclusively the hydroxycinnamic acids, such as p-coumar ... | 2010 | 20333439 |
| the effects of lactobacillus buchneri with or without a homolactic bacterium on the fermentation and aerobic stability of corn silages made at different locations. | whole-plant corn (31 to 39% dry matter) from several locations was chopped, treated with nothing (u), lactobacillus buchneri 40788 (4 x 10(5) cfu/g; lb), or l. buchneri (4 x 10(5) cfu/g) and pediococcus pentosaceus (1 x 10(5) cfu/g; lbpp), and packed into quadruplicate 20-l silos to determine their effects on silage fermentation and aerobic stability after 120 d of storage. the experiment was a randomized complete block design with main effects of treatment (t), block (location; l), and t x l in ... | 2010 | 20338439 |
| use of the yeast pichia pastoris as an expression host for secretion of enterocin l50, a leaderless two-peptide (l50a and l50b) bacteriocin from enterococcus faecium l50. | in this work, we report the expression and secretion of the leaderless two-peptide (entl50a and entl50b) bacteriocin enterocin l50 from enterococcus faecium l50 by the methylotrophic yeast pichia pastoris x-33. the bacteriocin structural genes entl50a and entl50b were fused to the saccharomyces cerevisiae gene region encoding the mating pheromone alpha-factor 1 secretion signal (mfalpha1(s)) and cloned, separately and together (entl50ab), into the p. pastoris expression and secretion vector ppic ... | 2010 | 20348300 |
| robustness of lactobacillus plantarum starters during daily propagation of wheat flour sourdough type i. | this study aimed at investigating the robustness of selected sourdough strains of lactobacillus plantarum. seven strains were singly used as sourdough type i starters under daily back-slopping propagation (ten days) using wheat flour. cell numbers of presumptive lactic acid bacteria varied slightly (median values of 9.13-9.46 log cfu g(-1)) between and within started sourdoughs, as well as the acidifying activity (median values of 1.24-1.33). after three days also the control sourdough (unstarte ... | 2010 | 20688231 |
| control of escherichia coli o157:h7 in corn silage with or without various inoculants: efficacy and mode of action. | this study aimed to evaluate the effectiveness of 3 commercial bacterial inoculants at controlling escherichia coli o157:h7 in corn silages during ensiling and feedout phases of silage production. a second objective was to determine whether the inoculants exhibited and transferred antibacterial activity against e. coli o157:h7 to the silages. chopped corn forage was ensiled after treatment with the following: distilled water (control); 5 x 10(5) cfu/g of e. coli o157:h7 (ec); ec and 1 x 10(6) cf ... | 2010 | 20172231 |
| the effect of pediococcus acidilactici on the gut microbiota and immune status of on-growing red tilapia (oreochromis niloticus). | aim: to assess pediococcus acidilactici as a dietary supplement for on-growing red tilapia (oreochromis niloticus). methods and results: tilapia were fed either a control diet or control diet supplemented with ped. acidilactici at 10(7) cfu g(-1) for 32 days. ped. acidilactici colonized the intestinal tract and significantly affected the intestinal microbial communities. pcr-dgge revealed direct antagonism of gastric ped. acidilactici with an endogenous uncultured bacterium during a period of re ... | 2010 | 20353430 |
| quorum sensing in sourdough lactobacillus plantarum dc400: induction of plantaricin a (plna) under co-cultivation with other lactic acid bacteria and effect of plna on bacterial and caco-2 cells. | this work aimed at showing the effect of pheromone plantaricin a (plna) by lactobacillus plantarum dc400 towards other sourdough lactic acid bacteria and the potential of plna to protect the function of the human intestinal barrier. growth and survival of sourdough lactic acid bacteria were differently affected by co-cultivation with l. plantarum dc400. compared to mono-cultures, lactobacillus sanfranciscensis dppma174 and pediococcus pentosaceus 2xa3 showed growth inhibition and decreased viabi ... | 2010 | 20354993 |
| evaluation of beneficial attributes for phytate-degrading pediococcus pentosaceus cfr r123. | in this study we have screened and selected potent phytate-degrading lactic acid bacteria (lab), and evaluated their beneficial attributes. around 60 lab strains were isolated from several cereal- and pulse-based conventional fermented preparations. they were screened for their ability to degrade myo-inositol hexakisphosphate (ip6) by cobalt chloride qualitative staining method (plate assay). one of the cultures, pediococcus pentosaceus cfr r123, was capable of degrading both calcium and sodium ... | 2010 | 21831762 |
| characterisation of the heat-stable bacteriocin-producing and vancomycin-sensitive pediococcus pentosaceus cfr b19 isolated from beans. | a bacteriocin-producing lactic culture with antilisterial activity was isolated from beans and identified as pediococcus pentosaceus cfr b19. it was able to grow and produce bacteriocin at 41 °c but not at 50 °c. this isolate was found to be sensitive to vancomycin and produced heat-stable (at 121 °c for 15 min) bacteriocin. molecular weight of the purified bacteriocin was found to be ~4.8 kda. this isolate can be used as a starter culture or co-culture in fermented milk products and the bacteri ... | 2010 | 21840803 |
| resolution of persistent pediococcus bacteremia with daptomycin treatment: case report and review of the literature. | a case of bacteremia caused by pediococcus acidilactici occurring in a patient with metastatic adenocarcinoma of the gallbladder is described. the bacteremia persisted despite an antimicrobial regimen of vancomycin, ciprofloxacin, metronidazole, and caspofungin. within 36 h of switching the vancomycin to daptomycin (6 mg/kg per day), the bacteremia resolved and the patient improved clinically. previous reports of human pediococcal infection are also reviewed. | 2010 | 19081696 |
| characterisation and transferability of antibiotic resistance genes from lactic acid bacteria isolated from irish pork and beef abattoirs. | lactic acid bacteria isolated from irish pork and beef abattoirs were analysed for their susceptibility to antimicrobials. thirty-seven isolates (12 enterococci, 10 lactobacilli, 8 streptococci, 3 lactococci, 2 leuconostoc, and 2 pediococci) were examined for phenotypic resistance using the e-test and their minimum inhibitory concentration to a panel of six antibiotics (ampicillin, chloramphenicol, erythromycin, streptomycin, tetracycline, and vancomycin) was recorded. the corresponding genetic ... | 2010 | 20074643 |
| assessment of potential probiotic- and starter properties of pediococcus spp. isolated from turkish-type fermented sausages (sucuk). | in this study, the metabolic activities of five strains of amount of pediococcus spp. in terms of the quantities they produced of lactic acid, hydrogen peroxide, exopolysaccharides and proteolytic activity were determined. lactic acid levels produced by these strains were found to be in the range of 2.5-5.6 mg/ml. all strains produced hydrogen peroxide. the p. pentosaceus z13p strain produced the maximum amount (0.25 mg/ml) of proteolytic activity. exopolysaccharide (eps) production by the pedio ... | 2010 | 20134248 |
| effects of a probiotic intervention in acute canine gastroenteritis--a controlled clinical trial. | to evaluate the effect of a probiotic product in acute self-limiting gastroenteritis in dogs. | 2010 | 20137007 |
| yield and chemical composition of fractions from fermented shrimp biowaste. | chemical composition of chitinous residue and fermentation liquor fractions, obtained from fermented shrimp biowaste, was evaluated in order to explore their potential for further utilization. lyophilization of the liquor fraction obtained after fermentation resulted in a powder rich in both protein (30%) and carotenoids (217.18 +/- 2.89 microg/g). the yield of chitinous residue was 44% (w/w) whereas the yield of lyophilized powder was >25% (w/v). about 69% of total carotenoids were recovered by ... | 2010 | 19723823 |
| pediocin production in milk by pediococcus acidilactici in co-culture with streptococcus thermophilus and lactobacillus delbrueckii subsp. bulgaricus. | the production of pediocin in milk by pediococcus acidilactici was evaluated in co-culture with the dairy fermentation cultures streptococcus thermophilus and lactobacillus delbrueckii ssp. bulgaricus. the cultures were tested singly and in different combinations in milk (0 or 2% fat content) during incubation at 40 degrees c for up to 10 h. cell-free milk samples taken every 60 min were tested for bacteriocin activity against listeria monocytogenes. pediocin activity was not detectable when p. ... | 2010 | 19844751 |
| genotypic diversity of lactic acid bacteria isolated from african traditional alkaline-fermented foods. | to identify and compare lactic acid bacteria (lab) isolated from alkaline fermentations of cassava (manihot esculenta crantz) leaves, roselle (hibiscus sabdariffa) and african locust bean (parkia biglobosa) seeds for production of, respectively, ntoba mbodi, bikalga and soumbala. | 2010 | 19895650 |
| association of beta-glucan endogenous production with increased stress tolerance of intestinal lactobacilli. | the exopolysaccharide beta-glucan has been reported to be associated with many health-promoting and prebiotic properties. the membrane-associated glycosyltransferase enzyme (encoded by the gtf gene), responsible for microbial beta-glucan production, catalyzes the conversion of sugar nucleotides into beta-glucan. in this study, the gtf gene from pediococcus parvulus 2.6 was heterologously expressed in lactobacillus paracasei nfbc 338. when grown in the presence of glucose (7%, wt/vol), the recomb ... | 2010 | 19933353 |
| susceptibility of meat starter cultures to antimicrobials used in food animals in canada. | lactic acid bacteria (lab) are extensively used in the food industry for fermentation processes. however, it is possible that these bacteria may serve as a reservoir for antibiotic resistance genes that can be transferred to pathogens, giving rise to public health concerns. animal operations that use antimicrobials as growth promotants have been linked to the origin of resistance due to the selective effect of low levels of antimicrobial used in this management strategy. the objective of this st ... | 2010 | 20501043 |
| pelagic fish hydrolysates as peptones for bacterial culture media. | for several years in the quebec fisheries' industry, landings of pelagic fish have been calculated at over 4000 tons. these under-exploited species, rich in lipids and proteins, could be used in valuable new products. in the present study, hydrolysates of mackerel and herring were produced and utilized as sources of peptones in the formulation of new bacterial culture media. the molecular weight distribution analysis showed that molecules present in the hydrolysates were lower than 1300 da for h ... | 2009 | 19940932 |
| characterization of anti-listeria bacteriocins isolated from shellfish: potential antimicrobials to control non-fermented seafood. | this work had as main objectives to characterize two bacteriocins produced by lactic acid bacteria (lab) previously isolated from non-fermented seafood, in order to evaluate their potential as new food protective agents. the two bacteriocinogenic isolates were identified by polymerase chain reaction (pcr) using genus- and species-specific primers, and confirmed by 16s rdna sequencing, as enterococcus faecium and pediococcus pentosaceus. the antimicrobial spectrum of each strain included several ... | 2009 | 19081155 |
| effect of applying molasses or inoculants containing homofermentative or heterofermentative bacteria at two rates on the fermentation and aerobic stability of corn silage. | this study determined how the fermentation and aerobic stability of corn silage are affected by treatment with molasses or 2 dual-purpose inoculants applied at or above the recommended rate. corn forage (dekalb 69-70) was harvested at 39% dry matter (dm) and ensiled after treatment with no additives (control, con), molasses (mol), buchneri 500 inoculant, or pioneer 11c33 inoculant. molasses was applied at 3% of forage dm. buchneri 500 was applied at the recommended rate of 8 mg/kg fresh forage t ... | 2009 | 19164681 |
| importance of tetrahydroiso alpha-acids to the microbiological stability of beer. | while beer provides a very stable microbiological environment, a few niche microorganisms are capable of growth in malt, wort, and beer. the production of off-flavors and development of turbidity in the packaged product are due to the growth and metabolic activity of wild yeast, certain lactic acid bacteria (lab) and anaerobic gram-negative bacteria. beer also contains bitter hop compounds, which are toxic to gram-positive and gram-negative bacteria, and contribute to preventing the spoilage of ... | 2009 | 19714985 |
| assessment of cell surface properties and adhesion potential of selected probiotic strains. | to evaluate the physicochemical cell surface and adhesive properties of selected probiotic strains for human use. | 2009 | 19725886 |
| susceptibility of pediococcus isolates to antimicrobial compounds in relation to hop-resistance and beer-spoilage. | though important in the context of food microbiology and as potential pathogens in immuno-compromised humans, bacterial isolates belonging to the genus pediococcus are best known for their association with contamination of ethanol fermentation processes (beer, wine, or fuel ethanol). use of antimicrobial compounds (e.g., hop-compounds, penicillin) by some industries to combat pediococcus contaminants is long-standing, yet knowledge about the resistance of pediococci to antimicrobial agents is mi ... | 2009 | 19735560 |
| rich nutrition from the poorest - cereal fermentations in africa and asia. | cereal fermentations in africa and asia involve mainly the processing of maize, rice, sorghum and the millets. lactic acid bacteria (lactobacillus, pediococcus), enterobacter spp., yeasts (candida, debaryomyces, endomycopsis, hansenula, pichia, saccharomyces and trichosporon spp.) and filamentous fungi (amylomyces, aspergillus, mucor, and rhizopus spp.) contribute to desirable modifications of taste, flavour, acidity, digestibility, and texture in non-alcoholic beverages (e.g., uji, and ben-saal ... | 2009 | 19747601 |
| the development of lactic acid bacteria and lactobacillus buchneri and their effects on the fermentation of alfalfa silage. | this study was conducted to document the development of populations of lactic acid bacteria (lab) and lactobacillus buchneri in alfalfa silage treated with various inoculants. wilted and chopped alfalfa (45% dry matter) was treated with 1) distilled water (untreated, u), 2) lactobacillus buchneri 40788 (4 x 10(5) cfu/g; lb), or 3) l. buchneri 40788 (4 x 10(5) cfu/g) and pediococcus pentosaceus (1 x 10(5) cfu/g; lbpp). forages were packed into triplicate vacuum-sealed, nylon-polyethylene bags per ... | 2009 | 19762819 |
| pediocin a improves growth performance of broilers challenged with clostridium perfringens. | the aim of this study was to investigate the efficacy of the anticlostridial pediocin a from pediococcus pentosaceus fbb61 to contain negative effects associated to clostridium proliferation in broilers, through 2 subsequent investigations. in the first study, 36 ross 508 broilers were divided into 3 groups and fed for 21 d as follows: the control diet (ctr), the control diet supplemented with supernatant filtrate of a culture of p. pentosaceus fbb61-2 (bac-, isogenic mutant nonproducing pedioci ... | 2009 | 19762869 |
| evaluation of lactic acid bacteria for sourdough fermentation of amaranth. | spontaneous fermented sourdoughs prepared from five amaranth flours were investigated for the presence of lactic acid bacteria predominating the autochthonous microbiota and thus may be suitable as starter cultures. the doughs were fermented with daily back-slopping on a laboratory scale at 30 degrees c for 10 days. each day, ph-values and total titratable acidity degrees were determined and samples were analyzed for lactic acid bacteria and yeasts by cultural methods. the identity of the strain ... | 2009 | 19783060 |
| genotypic characterization of non starter lactic acid bacteria involved in the ripening of artisanal bitto pdo cheese. | bitto of valchiavenna, an artisanal italian cheese produced without the addition of any starter cultures, has been attributed a protected designation origin (pdo) cheese, but the strain composition of the natural microbial population colonizing this traditional dairy product is still unknown. to obtain preliminary information on the non starter lactic acid bacteria involved in its ripening, a total of 136 nslab isolates, randomly selected from mrs and m17 agar plates, were collected from three d ... | 2009 | 19810037 |
| lethality of home-style dehydrator processes against escherichia coli o157:h7 and salmonella serovars in the manufacture of ground-and-formed beef jerky and the potential for using a pathogen surrogate in process validation. | ground-and-formed beef jerky can be made easily at home with ground beef and kits that include spice, cure, and jerky-forming equipment. ground beef poses inherent risks of illness due to escherichia coli o157:h7 and salmonella contamination, making adequate pathogen lethality important in jerky manufacturing. we evaluated the effectiveness of drying regimes at eliminating e. coli o157:h7 and salmonella in seasoned ground-and-formed beef jerky manufactured with three home-style dehydrators and o ... | 2009 | 19833027 |
| development of a pcr assay for the strain-specific identification of probiotic strain lactobacillus paracasei impc2.1. | recent investigations clearly indicate that the probiotic bacterium lactobacillus paracasei impc2.1 can be incorporated into vegetables to obtain innovative probiotic foods whose marketing has been authorized by the italian ministry of health. in this study, strain impc2.1 was characterized at a molecular level in order to define its taxonomic position and to develop a pcr test for strain-specific identification. molecular methods, such as 16s rrna gene sequencing and multiplex pcr, have provide ... | 2009 | 19833402 |
| identification of lactic acid bacteria isolated from tarhana, a traditional turkish fermented food. | tarhana is a traditional fermented product produced from a mixture of spontaneously fermented yogurt and wheat flour in turkey. the aims of the present study were to enumerate and identify for the first time by molecular biology-based methods predominant lactic acid bacteria (lab) isolated during processing of tarhana. samples were collected from eight different regions of turkey. in order to explore the relationship between raw material and the microbiology of tarhana, yogurt and wheat flour we ... | 2009 | 19703719 |
| characterization of prs5: a theta-type plasmid found in a strain of pediococcus pentosaceus isolated from wine that can be used to generate cloning vectors for lactic acid bacteria. | the nucleotide sequence of prs5 (10153bp) is reported. through sequence analysis, 9 open reading frames (orfs) were identified and the following features observed: a region likely involved in replication whose structural features indicate that prs5 belongs to the pucl287 group of theta-type replicons, and hypothetical proteins putatively involved in plasmid copy number control, restriction-modification system, toxin-antitoxin system and a putative integrase. shuttle vectors for escherichia coli ... | 2009 | 19027788 |
| isolation and characterization of acid-sensitive mutants of pediococcus acidilactici. | acid-sensitive mutants of pediococcus acidilactici bcc 9545, a starter culture of the thai fermented pork sausage nham, were isolated as spontaneous neomycin resistant mutants. the mutants generally produced less acid and acidified the culture media less than the parent strain in a 72 h culturing period. interestingly, the atpase activities of the mutants did not differ considerably from that of the parent strain in acidic conditions. it was also found that the internal ph values of the mutant s ... | 2009 | 19028310 |
| effect of autochthonous lactic acid bacteria starters on health-promoting and sensory properties of tomato juices. | strains of lactobacillus plantarum, weissella cibaria/confusa, lactobacillus brevis, pediococcus pentosaceous, lactobacillus sp. and enterococcus faecium/faecalis were identified from raw tomatoes by biolog system, partial 16s rrna gene sequence and subjected to typing by random amplified polymorphic dna-polymerase chain reaction (rapd-pcr) analysis. ten autochthonous strains were singly used to ferment tomato juice (tj) via a protocol which included fermentation at 25 degrees c for 17 h and fur ... | 2009 | 19028404 |
| administration of pediococcus acidilactici or saccharomyces cerevisiae boulardii modulates development of porcine mucosal immunity and reduces intestinal bacterial translocation after escherichia coli challenge. | in this study, the influence of the probiotics, pediococcus acidilactici (pa) and saccharomyces cerevisiae boulardii (scb), on intestinal immune traits and resistance to enterotoxigenic escherichia coli (etec) infection was evaluated in pigs. two weeks before farrowing, 30 sows and their future litters were allocated to the following treatments: 1) control group without antibiotic or probiotic treatment (ctrl), 2) control with antibiotic (tiamulin) added to weanling feed (abt), or litters treate ... | 2009 | 19028865 |
| bacterial spoilage of wine and approaches to minimize it. | bacteria are part of the natural microbial ecosystem of wine and play an important role in winemaking by reducing wine acidity and contributing to aroma and flavour. conversely, they can cause numerous unwelcome wine spoilage problems, which reduce wine quality and value. lactic acid bacteria, especially oenococcus oeni, contribute positively to wine sensory characters, but other species, such as lactobacillus sp. and pediococcus sp can produce undesirable volatile compounds. consequences of bac ... | 2009 | 19141041 |
| isolation of bovine intestinal lactobacillus plantarum and pediococcus acidilactici with inhibitory activity against escherichia coli o157 and f5. | the growth rate of bovine lactic acid bacteria (lab) in five different culture conditions, and their inhibitory activity against escherichia coli o157 and f5 in two assays was assessed to identify lab for potential prophylactic use in cattle. | 2009 | 19200307 |
| inactivation of oenological lactic acid bacteria (lactobacillus hilgardii and pediococcus pentosaceus) by wine phenolic compounds. | to investigate the inactivation properties of different classes of phenolic compounds present in wine against two wine isolates of lactobacillus hilgardii and pediococcus pentosaceus, and to explore their inactivation mechanism. | 2009 | 19486417 |
| detection of arc genes related with the ethyl carbamate precursors in wine lactic acid bacteria. | trace amounts of the carcinogen ethyl carbamate can appear in wine by the reaction of ethanol with compounds such as citrulline and carbamyl phosphate, which are produced from arginine degradation by some wine lactic acid bacteria (lab). in this work, the presence of arc genes for the arginine-deiminase pathway was studied in several strains of different species of lab. their ability to degrade arginine was also studied. to detect the presence of arc genes, degenerate primers were designed from ... | 2009 | 19219988 |
| broth and agar hop-gradient plates used to evaluate the beer-spoilage potential of lactobacillus and pediococcus isolates. | identification of the beer-spoilage lactobacillus and pediococcus bacteria has largely taken two approaches; identification of spoilage-associated genes or identification of specific species of bacteria regardless of ability to grow in beer. the problem with these two approaches is that they are either overly inclusive (i.e., detect all bacteria of a given species regardless of spoilage potential) or overly selective (i.e., rely upon individual, putative spoilage-associated genes). our goal was ... | 2009 | 19187996 |
| in vivo study on the effectiveness of pediocin pa-1 and pediococcus acidilactici ul5 at inhibiting listeria monocytogenes. | the anti-listerial effect of pediocin pa-1 and its producing strain, pediococcus acidilactici ul5, was investigated in vivo using an icr mouse model. the effect of intra-gastric administration of a single dose of p. acidilactici ul5 (4 x 10(10) cfu/animal) on the propagation of listeria monocytogenes lsd348 in intestine, liver and spleen was negligible. p. acidilactici ul5 did not appear competitive with the mouse intestinal flora and was not detectable in fecal samples collected two days after ... | 2009 | 19541383 |
| chemical and rheological properties of the beta-glucan produced by pediococcus parvulus 2.6. | some physicochemical and rheological properties of the exopolysaccharide (eps) produced by pediococcus parvulus 2.6 were examined. structural characterization by nmr ((1)h and 2d-cosy) showed that the same eps, a 2-substituted (1,3)-beta-d-glucan, was synthesized irrespective of sugar source used for growth (glucose, fructose, or maltose). the molecular masses of these beta-glucans were always very high (>10(6) da) and influenced by the culture medium or sugar source. the steady shear rheologica ... | 2009 | 19219990 |
| short communication: an evaluation of the effectiveness of lactobacillus buchneri 40788 to alter fermentation and improve the aerobic stability of corn silage in farm silos. | the objective of this study was to determine if the effects of inoculation with lactobacillus buchneri 40788 were detectable when applied to whole-plant corn stored in farm silos. corn silage was randomly sampled from farms in wisconsin, minnesota, and pennsylvania, and was untreated (n = 15) or treated with an inoculant (n = 16) containing l. buchneri 40788 alone or this organism combined with pediococcus pentosaceus during may and june 2007. corn silage that was removed from the silo face duri ... | 2009 | 19233810 |
| clinical efficacy of halophilic lactic acid bacterium tetragenococcus halophilus th221 from soy sauce moromi for perennial allergic rhinitis. | recently, some common foods in daily life, especially lactic acid bacteria, have been found to have anti-allergic effects. we previously isolated a halophilic lactic acid bacterium, tetragenococcus halophilus th221, from soy sauce moromi, a mixture of koji and salt solution, and showed that it possesses an immunomodulatory activity that promotes t helper type 1 immunity. | 2009 | 19240374 |
| probiotics, critical illness, and methodologic bias. | it has been proposed that probiotics can favorably influence the course of critically ill patients. to address this question, a limited systematic review was undertaken (medline search for articles published in english) to identify randomized, controlled trials that compared a group of critically ill patients taking probiotics with a group that did not. ten such trials, mostly with high risks of methodologic bias, were identified. when the data were combined, the probiotics did not appear to inf ... | 2009 | 19244148 |
| reclassification of pediococcus dextrinicus (coster and white 1964) back 1978 (approved lists 1980) as lactobacillus dextrinicus comb. nov., and emended description of the genus lactobacillus. | the taxonomic status of pediococcus dextrinicus is described and transfer of the species to the genus lactobacillus, with the name lactobacillus dextrinicus comb. nov., is proposed. this reclassification is supported by multilocus sequence analysis of the 16s rrna gene and cpn60, phes, reca and rpoa proteins. the mode of cell division and existing phenotypic information also show that p. dextrinicus does not belong to the genus pediococcus, but rather to the genus lactobacillus. as such, we prop ... | 2009 | 19244449 |
| pediocin a modulates intestinal microflora metabolism in swine in vitro intestinal fermentations. | the aim of this study was to investigate in vitro the effects of pediocin a [a bacteriocin produced by lactic acid bacteria (lab) pediococcus pentosaceus fbb61] on microbial metabolism in the small and large intestine of pigs. pediocin a was partially purified by ion exchange chromatography and added to an in vitro fermentation system. the intestinal inoculum was collected from pigs immediately after slaughter, diluted with a buffer, and dispensed into fermentation syringes and vessels of the 2 ... | 2009 | 19251932 |
| validation of ground-and-formed beef jerky processes using commercial lactic acid bacteria starter cultures as pathogen surrogates. | beef jerky has been linked to multiple outbreaks of salmonellosis and escherichia coli o157:h7 infection over the past 40 years. with increasing government scrutiny of jerky-making process lethality, a simple method by which processors can easily validate the lethality of their ground-and-formed beef jerky process against salmonella' and e. coli o157:h7 is greatly needed. previous research with whole-muscle beef jerky indicated that commercial lactic acid bacteria (lab) may be more heat resistan ... | 2009 | 19610334 |
| selectivity and antimicrobial action of bovine lactoferrin derived peptides against wine lactic acid bacteria. | in this study, the antibacterial activities of a bovine lactoferrin pepsin hydrolysate (lfh) and a synthetic peptide derived from bovine lactoferricin (lfcinb(17-31)) have been evaluated against oenococcus oeni and three additional lactic acid bacteria (lab) known to cause spoilage during winemaking processes. inhibition of bacterial growth was demonstrated in vitro in synthetic broth media (mrs) for both lfh and lfcinb(17-31). the bactericidal activity of the synthetic peptide was also assayed ... | 2009 | 19269579 |
| a lytic enzyme cocktail from streptomyces sp. b578 for the control of lactic and acetic acid bacteria in wine. | beside yeasts, lactic acid bacteria (lab) are the most abundant microbes in must during vinification. whereas oenococcos oeni is commercially used as a starter culture for the biological acid reduction in wines, other species are responsible for different types of wine spoilage. members of the genera pediococcus, weissella, leuconostoc, and lactobacillus are producers of exopolysaccharide slimes, biogenic amines, acetic acid, and other off-flavors. in order to control microbial growth, different ... | 2009 | 19277643 |
| development of a new method for the detection of lactic acid bacteria capable of protecting ham against enterobacteriaceae. | challenge trials seem to be the best assessment approach to evaluate the potency of food protective cultures. however, this method is time consuming and often difficult to implement. here, we describe the development of the 'sequential culturing method', a new method for the screening of strains as protective cultures. | 2009 | 19291212 |
| co-cultivation of a bacteriocin-producing mixed culture of bifidobacterium thermophilum rbl67 and pediococcus acidilactici uva1 isolated from baby faeces. | we investigated the kinetics of growth, metabolism and antimicrobial activity of bifidobacterium thermophilum rbl67 and pediococcus acidilactici uva1 previously isolated as a consortium from human baby faeces and producing antimicrobial proteinaceous compounds. | 2009 | 19298509 |
| modification of azo dyes by lactic acid bacteria. | the ability of lactobacillus casei and lactobacillus paracasei to modify the azo dye, tartrazine, was recently documented as the result of the investigation on red coloured spoilage in acidified cucumbers. fourteen other lactic acid bacteria (lab) were screened for their capability to modify the food colouring tartrazine and other azo dyes of relevance for the textile industry. | 2009 | 19302317 |
| characterization of the beneficial properties of lactobacilli isolated from bullfrog (rana catesbeiana) hatchery. | the present work addresses the isolation and partial identification of the microbial population of a r. catesbeiana hatchery in spring and summer as well as some beneficial properties of lactobacillus strains isolated in different seasons and hatchery areas. the bacterial population was grouped into the following taxa: lactobacillus spp., pediococcus spp., enterococcus faecalis and ent. faecium, and enterobacteriaceae (enterobacter spp., escherichia coli) while pseudomonas aeruginosa and staphyl ... | 2009 | 19306110 |
| production of shelf-stable ranch dressing using high-pressure processing. | high-pressure processing (hpp) can reduce or eliminate microorganisms of concern in food without deteriorating product quality; however, quality benefits must justify the substantial capital investment for the utilization of this technology. hpp is particularly a beneficial preservation technology for products damaged by thermal treatments or when product quality could be improved by reformulation to raise ph or eliminate chemical preservatives. the primary objectives of this study were to deter ... | 2009 | 19323763 |
| characterization and application of a native lactic acid bacterium isolated from tannery fleshings for fermentative bioconversion of tannery fleshings. | lactic acid bacteria (lab) species isolated from limed and delimed tannery fleshings (tf) were evaluated for their fermentation efficiency and antibacterial property. the native lab isolates efficiently fermented tf and resulted in a fermented mass with antioxidant properties, indicating their potential for effective eco-friendly bioconversion of tf. from among the lab isolated, a proteolytic isolate showing better antimicrobial spectrum and reasonably good fermentation efficiency was identified ... | 2009 | 19333593 |
| synthesis and antibacterial activity of nisin-containing block copolymers. | nisin, an antibacterial peptide proven to be an effective inhibitor of gram-positive bacteria, was incorporated into novel block copolymer constructs and tested for retained antibacterial activity. covalent coupling was achieved by chemical modification of the n-terminal isoleucine to introduce a thiol group. thiolated-nisin derivatives were then linked to poly[ethylene oxide]-poly[propylene oxide]-poly[ethylene oxide] (peo-ppo-peo) triblocks that had been end-activated such that terminal hydrox ... | 2009 | 19358262 |
| pediococcus lolii sp. nov., isolated from ryegrass silage. | a gram-positive, coccus-shaped, lactic acid bacterium, strain ngri 0510q(t), was isolated from ryegrass silage produced in okinawa prefecture, japan. the cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. the strain is homofermentative and produces d- and l-lactic acid from glucose. phylogenetic analysis based on 16s rrna gene sequences revealed that strain ngri 0510q(t) belongs to the genus pediococcus and clusters within the pediococcus acidilactici and pediococcus pentosac ... | 2009 | 19406783 |
| pyrosequencing of tagged 16s rrna gene amplicons for rapid deciphering of the microbiomes of fermented foods such as pearl millet slurries. | pearl millet slurries, mixed with groundnuts or not, were chosen as a model to investigate the feasibility of obtaining a rapid overview of community structure and population dynamics of fermented foods using pyrosequencing of tagged 16s rrna gene amplicons. from 14 fermented samples collected either in a traditional small-scale processing unit in burkina faso or at laboratory scale, 137,469 sequences of bacterial 16s rrna gene amplicons were characterized. except for a few proteobacteria, almos ... | 2009 | 19411407 |
| bacteriocin production by pediococcus pentosaceus isolated from marula (scerocarya birrea). | strain st44am, isolated from marula, was identified as pediococcus pentosaceus based on biochemical tests, sugar fermentation reactions (api 50chl), pcr with species-specific primers and 16s rdna sequencing. strain st44am produces a 6.5 kda class iia bacteriocin, active against lactic acid bacteria, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, listeria innocua, listeria ivanovii subsp. ivanovii and listeria monocytogenes. the peptide is inactivated by proteolytic enzymes, but ... | 2009 | 19446352 |
| evaluation of a facile method of template dna preparation for pcr-based detection and typing of lactic acid bacteria. | the objective of our investigation was to develop a convenient and reliable method of generating template dna for routine pcr-based detection and typing of lactic acid bacteria (lab). template dna extracted from lactobacillus, lactococcus, pediococcus and leuconostoc using a combination of urea, sds and naoh yielded amplicons of expected size in pcr with genus-specific primers. apart from lab, the proposed method could also be adopted to generate pcr-compatible template dna from a number of gram ... | 2009 | 19465247 |
| lactic acid bacteria as reducing and capping agent for the fast and efficient production of silver nanoparticles. | there is a growing demand for silver-based biocides, including both ionic silver forms and metallic nanosilver. the use of metallic nanosilver, typically chemically produced, faces challenges including particle agglomeration, high costs, and upscaling difficulties . additionally, there exists a need for the development of a more eco-friendly production of nanosilver. in this study, gram-positive and gram-negative bacteria were utilized in the non-enzymatic production of silver nanoparticles via ... | 2009 | 19488750 |
| probiotic properties of the 2-substituted (1,3)-beta-d-glucan-producing bacterium pediococcus parvulus 2.6. | exopolysaccharides have prebiotic potential and contribute to the rheology and texture of fermented foods. here we have analyzed the in vitro bioavailability and immunomodulatory properties of the 2-substituted (1,3)-beta-d-glucan-producing bacterium pediococcus parvulus 2.6. it resists gastrointestinal stress, adheres to caco-2 cells, and induces the production of inflammation-related cytokines by polarized macrophages. | 2009 | 19465528 |
| screening, selection and characterization of phytic acid degrading lactic acid bacteria from chicken intestine. | this study was undertaken to screen and select potent phytate degrading lactic acid bacteria and to evaluate their additional characteristic features. forty lactic acid bacterial strains were isolated from different sources and screened for their ability to degrade myo-inositol hexaphosphate or ip(6) by cobalt chloride staining (plate assay) method, using calcium or sodium salt of phytic acid as substrate. all the forty isolates were able to degrade calcium phytate. however, only two pediococcus ... | 2009 | 19481282 |
| biodiversity of lactic acid bacteria in french wheat sourdough as determined by molecular characterization using species-specific pcr. | the lactic acid microflora of nine traditional wheat sourdoughs from the midi-pyrénées area (south western france) was previously isolated and preliminary characterized using conventional morphological and biochemical analysis. however, such phenotypic methods alone are not always reliable and have a low taxonomic resolution for identification of lactic acid bacteria species. in the present study, a total of 290 lab isolates were identified by pcr amplification using different sets of specific p ... | 2009 | 19651455 |
| isolation and identification of spoilage microorganisms using food-based media combined with rdna sequencing: ranch dressing as a model food. | investigating microbial spoilage of food is hampered by the lack of suitable growth media and protocols to characterize the causative agents. microbial spoilage of salad dressing is sporadic and relatively unpredictable, thus processors struggle to develop strategies to minimize or prevent spoilage of this product. the objectives of this study were to (i) induce and characterize spoilage events in ranch-style dressing as a model food, and (ii) isolate and identify the causative microorganisms us ... | 2009 | 19269562 |
| [lactic acid bacteria diversity in fermented cabbage estimated by culture-dependent and-independent methods]. | to study the diversity of lactic acid bacteria (lab) and dominant lab in fermented cabbage. | 2009 | 19623964 |
| polyphasic taxonomic studies of lactic acid bacteria associated with tunisian fermented meat based on the heterogeneity of the 16s-23s rrna gene intergenic spacer region. | the objective of this work was to investigate the structure and diversity of lactic acid bacteria (lab) communities in traditionally fermented meat collected from different areas of tunisia. a polyphasic study, which involves phenotypic tests and ribosomal dna-based techniques, was used to identify gram-positive and catalase-negative isolates. pcr amplification of the 16s-23s rdna isr of 102 isolates and other reference lab strains gave (1) one type of rrn operon (m-isr) for lactococci, (2) two ... | 2009 | 19669730 |
| diversity of lactic acid bacteria in suan-tsai and fu-tsai, traditional fermented mustard products of taiwan. | fu-tsai and suan-tsai are spontaneously fermented mustard products traditionally prepared by the hakka tribe of taiwan. we chose 5 different processing stages of these products for analysis of the microbial community of lactic acid bacteria (lab) by 16s rrna gene sequencing. from 500 lab isolates we identified 119 representative strains belonging to 5 genera and 18 species, including enterococcus (1 species), lactobacillus (11 species), leuconostoc (3 species), pediococcus (1 species), and weiss ... | 2009 | 19700215 |
| taxonomic structure and monitoring of the dominant population of lactic acid bacteria during wheat flour sourdough type i propagation using lactobacillus sanfranciscensis starters. | the structure and stability of the dominant lactic acid bacterium population were assessed during wheat flour sourdough type i propagation by using singly nine strains of lactobacillus sanfranciscensis. under back-slopping propagation with wheat flour type 0 f114, cell numbers of presumptive lactic acid bacteria varied slightly between and within starters. as determined by randomly amplified polymorphic dna-pcr and restriction endonuclease analysis-pulsed-field gel electrophoresis analyses, only ... | 2009 | 19088320 |
| molecular screening of wine lactic acid bacteria degrading hydroxycinnamic acids. | the potential to produce volatile phenols from hydroxycinnamic acids was investigated for lactic acid bacteria (lab) isolated from spanish grape must and wine. a pcr assay was developed for the detection of lab that potentially produce volatile phenols. synthetic degenerate oligonucleotides for the specific detection of the pdc gene encoding a phenolic acid decarboxylase were designed. the pdc pcr assay amplifies a 321 bp dna fragment from phenolic acid decarboxylase. the pdc pcr method was appl ... | 2009 | 19099460 |
| bio-ecological control of chronic liver disease and encephalopathy. | minimal encephalopathy was originally associated with chronic liver disease but is increasingly associated with most other chronic diseases and particularly with diabetes and also chronic disorders in other organs: kidneys, lungs, thyroid and with obesity. it is increasingly with dramatically increased and more or less permanent increase in systemic inflammation, most likely a result of western lifestyle. frequent physical exercise and intake of foods rich in vitamins, antioxidants, fibres, lact ... | 2009 | 19104922 |
| description of the microflora of sourdoughs by culture-dependent and culture-independent methods. | four types of sourdoughs (l, c, b, q) from artisanal bakeries in northern italy were studied using culture-dependent and culture-independent methods. in all samples, the yeast numbers ranged from 160 to 10(7)cfu/g, and the numbers of lactic acid bacteria (lab) ranged from 10(3) to 10(9)cfu/g. the isolated lab were sequenced, and a similarity was noted between two samples (c, q), both in terms of the species that were present and in terms of the percentage of isolates. in these two samples, lacto ... | 2009 | 19171253 |
| biogenic amine content in dry fermented sausages as influenced by a producer, spice mix, starter culture, sausage diameter and time of ripening. | sixteen types of dry fermented sausages were commercially produced as combinations of two producers (designated k and r), two starter cultures (pediococcus pentosaceus, c; lactobacillus curvatus+staphylococcus carnosus, f), two spicing mixtures (h; p) and two casing diameters (4.5cm, t; 7cm, w), and were sampled at days zero, 14, 28 (end of ripening), 49, 70, 91 and 112 (samples were stored at 15 degrees c and relative humidity of 70% between days 28 and 112). tyramine and putrescine content (y, ... | 2009 | 20416663 |
| phenotypic and genetic heterogeneity of lactic acid bacteria isolated from "alheira", a traditional fermented sausage produced in portugal. | the aim of this study was to evaluate the phenotypic and genetic heterogeneity of lactic acid bacteria (lab) isolated from "alheira", a fermented sausage produced in portugal. lab were identified to genus and species level by phenotypic characteristics, using genus or species-specific primers and sequencing of the gene encoding 16s rrna. two-hundred and eighty-three isolates were grouped into 14 species. lactobacillus plantarum was isolated from all sausages and enterococcusfaecalis from most of ... | 2009 | 20416703 |
| antagonistic activity of spent culture supernatants of lactic acid bacteria against helicobacter pylori growth and infection in human gastric epithelial ags cells. | we investigated the bactericidal activity and exclusion effect of 10 strains of lactic acid bacteria (lab) isolated from different commercial food products and infant feces against helicobacter pylori (h. pylori) in human gastric epithelial ags cells. antagonistic activity of spent culture supernatants (scs) from lab (lab-scs) was tested, and the content of organic acids in scs was analyzed with high-performance liquid chromatography (hplc). in addition, the bactericidal activities of lab-scs we ... | 2009 | 19723205 |
| optimized culture conditions for bacteriocin production by pediococcus acidilactici lab 5 and its characterization. | a strain of pediococcus acidilactici lab 5 was isolated from vacuum-packed fermented meat product, in order to obtain a novel bacteriocin from food-grade organisms. optimized culture conditions for bacteriocin production in different media (viz., mrs, tge, tge + buffer, tge + tween 80, and tge + tween 80 + buffer) and at different temperatures and ph conditions were reported. tge + tween 80 + buffer medium was found to be most effective for bacteriocin production (about 2400 au/ml) by this strai ... | 2008 | 21086723 |
| effect of processing methods and starter culture (staphylococcus xylosus and pediococcus pentosaceus) on proteolytic changes in turkish sausages (sucuk) during ripening and storage. | proteolytic changes in turkish sausages (sucuk) produced by two methods (heat processing and traditional) were determined during processing and storage for 90 days. the sausages were produced with or without starter culture in both methods. a mixture of staphylococcus xylosus and pediococcus pentosaceus was used as starter culture for their acidic and proteolytic characteristics. the major changes in proteolytic characteristics of sucuk took place during the fermentation stage, with an increase ... | 2008 | 22063339 |
| screening of lactic acid bacteria and bifidobacteria for potential probiotic use in iberian dry fermented sausages. | the purpose of this study was to select lactic acid bacteria and bifibobacteria strains as potential probiotic cultures during the processing of iberian dry fermented sausages. a total of 1000 strains were isolated from iberian dry fermented sausages (363), and human (337) and pig faeces (300) in different culture media. around 30% of these strains, mainly isolated from iberian dry fermented sausages in lamvab agar, were pre-selected for testing as potential probiotics by their ability to grow a ... | 2008 | 22063588 |