Publications

[septic shock fusobacterium necrophorum from origin gynecological at complicated an acute respiratory distress syndrome: a variant of lemierre's syndrome].	we report a case of a female patient of 47 years old who presents in a state of septic shock with acute insufficient respiratory complicated with syndrome of acute respiratory distress, together with a list of abdominal pain and polyarthralgia too. in her case of medical history, it is retained that she has had a intra-uterine device since 6 years without medical follow up. the initial thoraco-abdomino-pelvic scan shows a left ovarian vein thrombosis, as well as the opaqueness alveolus diffused ...	2011	21464014
microbiology of sinusitis.	most sinus infections are viral, and only a small proportion develops a secondary bacterial infection. rhinoviruses, influenza viruses, and parainfluenza viruses are the most common causes of sinusitis. the most common bacteria isolated from pediatric and adult patients with community-acquired acute purulent sinusitis are streptococcus pneumoniae, haemophilus influenzae, moraxella catarrhalis, and streptococcus pyogenes. staphylococcus aureus and anaerobic bacteria (prevotella and porphyromonas, ...	2011	21364226
analysis of symptomatic and asymptomatic primary root canal infections in adult norwegian patients.	this molecular study analyzed the microbiota of primary root canal infections from adult norwegian patients.	2011	21846535
extracellular deoxyribonuclease production by periodontal bacteria.	palmer lj, chapple ilc, wright hj, roberts a, cooper pr. extracellular deoxyribonuclease production by periodontal bacteria. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01451.x © 2011 john wiley & sons a/s background and objective: whilst certain bacteria have long been known to secrete extracellular deoxyribonuclease (dnase), the purpose in microbial physiology was unclear. recently, however, this enzyme has been demonstrated to confer enhanced virulence, enabling bacteria to evade the ...	2011	22150619
burden of emerging anaerobes in the maldi-tof and 16s rrna gene sequencing era.	the isolation of anaerobes from patients has declined in recent years, whereas their detection by molecular techniques has increased. in the present work, we analyzed the application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (maldi-tof ms) and 16s rrna gene sequencing to routine identification of anaerobes in clinical microbiology laboratory. we identified 544 isolates of 79 species by routine culture from deep samples in our hospital. maldi-tof ms allowed i ...	2011	21672636
a pilot study: microbiological conditions of the oral cavity in minipigs for peri-implantitis models.	as peri-implantitis is an emerging problem, the development of validated animal models is mandatory. the aim of this pilot study was to provide a first step in describing the normal oral flora of minipigs. in five minipigs, samples of the oral flora were collected with sterile cotton swabs from the buccal gingiva of the lower jaw. two swabs per animal were collected, followed by bacterial isolation under both aerobe and anaerobe conditions. microbiological analyses included biochemical tests, ma ...	2011	21540281
severe pneumonia and jaundice in a young man: an atypical presentation of an uncommon disease.	we present a patient with an atypical presentation of fusobacterium infection, the genus responsible for lemierre's syndrome. this syndrome, which often affects healthy, young people and can be fatal if not recognised and treated early, is defined as a history of recent oropharyngeal infection with clinical or radiological evidence of internal jugular vein thrombosis and isolation of anaerobic pathogens, mainly fusobacterium necrophorum. the history, presentation, investigations and management o ...	2011	21546562
effects of adding polyclonal antibody preparations on ruminal fermentation patterns and digestibility of cows fed different energy sources.	nine ruminally cannulated cows fed different energy sources were used to evaluate an avian-derived polyclonal antibody preparation (pap-mv) against the specific ruminal bacteria streptococcus bovis, fusobacterium necrophorum, clostridium aminophilum, peptostreptococcus anaerobius and clostridium sticklandii and monensin (mon) on ruminal fermentation patterns and in vivo digestibility. the experimental design was three latin squares 3 x 3 distinguished by the main energy source in the diet [dry-g ...	2011	21551346
lateral saccular laryngeal cyst and unexpected asphyxial death.	we present the case of a 41-year-old man with a herniated saccular laryngeal cyst in the left cervical region who died unexpectedly at home from acute asphyxia. a few days before death the patient complained an obvious palpable swelling on the left side of the neck. the autopsy showed an oval-shaped mass originating on the wall of the larynx and herniating into the extralaryngeal region. the critical reduction of the laryngeal lumen originated from an intense oedema of the laryngeal mucosa above ...	2011	20663624
effect of smoking on subgingival microflora of patients with periodontitis in japan.	smoking is a risk factor for periodontitis. to clarify the contribution of smoking to periodontitis, it is essential to assess the relationship between smoking and the subgingival microflora. the aim of this study was to gain an insight into the influence of smoking on the microflora of japanese patients with periodontitis.	2011	21208407
identification of periodontal pathogens and severity of periodontitis in patients with and without chronic kidney disease.	in this study of patients with chronic periodontitis (cp), the severity of the disease and the main periodontal pathogens identified in patients with chronic kidney disease (ckd) were compared with those detected in individuals without systemic disease.	2011	21211789
effect of nicotine, cotinine and cigarette smoke extract on the neutrophil respiratory burst.	to determine the effect of nicotine, cotinine and cigarette smoke extract (cse) on the neutrophil respiratory burst and their effect on activation of the nuclear factor-κb (nfκb) pathway in oral epithelium.	2011	21214612
fusobacterium nucleatum in periodontal health and disease.	the pathogenesis of periodontitis involves the interplay of microbiota present in the subgingival plaque and the host responses. inflammation and destruction of periodontal tissues are considered to result from the response of a susceptible host to a microbial biofilm containing gram-negative pathogens. antimicrobial peptides are important contributors to maintaining the balance between health and disease in this complex environment. these include several salivary antimicrobial peptides such as ...	2011	21220789
interactions between various microbes and ginseng botanicals.	three kinds of interactions occur between ginseng botanicals and microorganisms: a) spoilage of the botanical by various fungi (e.g., aspergillus, penicillium, alternaria, and eurotium species) and bacteria; b) transformation of ginsenosides into more bioactive forms by bacteria such as intrasporangium sp. gs603, microbacterium sp. gs514, caulobacter leidyia, bifidobacterium sp. int57, bifidobacterium sp. sj32, fusobacterium sp. and bacteroides sp., and moulds (e.g., aspergillus niger, fusarium ...	2011	21254831
[fusobacterium nucleatum isolated from a patient presenting lachrymal canaliculitis].	a 68-year-old woman presented with a painless inflammation of the right superior eyelid that had started several weeks before. the clinical diagnosis concluded in canaliculitis and the solid concretions were surgically extracted from the superior canalicula. the anaerobic bacteria fusobacterium nucleatum sp. nucleatum was isolated. signs dramatically regressed two weeks after surgery followed by one course of oral amoxicillin and clavulanic acid associated with topical tobramycin. the clinical s ...	2011	21255867
[a case of liver abscess caused by fusobacterium nucleatum in a patient with recurrent periodontal diseases].	fusobacteria are anaerobic gram-negative, non-spore forming bacilli found in normal flora of the oral cavity, urogenital tract, and gastrointestinal tract. fusobacterium nucleatum has been seldom reported as a cause of liver abscess, particularly in immunocompetent hosts. a 55-year-old man with frequent periodontal disease visited our hospital with intermittent fever and headache for 2 months. abdominal ct scan revealed an 8.2 × 6 cm mass in the right hepatic lobe with central low density. absce ...	2011	21258201
salivary infectious agents and periodontal disease status.	the potential of salivary microorganisms to diagnose periodontal disease and to guide periodontal treatment is a research topic of current interest. this study aimed to determine whether the salivary counts of periodontopathic microbes correlated with the periodontal pocket counts of the same infectious agents, and whether the salivary counts of the test infectious agents could distinguish among individuals with periodontal health and various types of periodontal disease.	2011	21261620
structure, viability and bacterial kinetics of an in vitro biofilm model using six bacteria from the subgingival microbiota.	there are few in vitro models available in the scientific literature for study of the structure, formation and development of the subgingival biofilm. the purpose of this study was to develop and validate an in vitro biofilm model, using representative selected bacteria from the subgingival microbiota.	2011	21261622
antimicrobial activity of diterpenes from viguiera arenaria against endodontic bacteria.	six pimarane-type diterpenes isolated from viguiera arenaria baker and two semi-synthetic derivatives were evaluated in vitro against a panel of representative microorganisms responsible for dental root canal infections. the microdilution method was used for the determination of the minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) against porphyromonas gingivalis, prevotella nigrescens, prevotella intermedia, prevotella buccae, fusobacterium nucleatum, bacteroi ...	2011	21233793
invasive potential of gut mucosa-derived fusobacterium nucleatum positively correlates with ibd status of the host.	background:: fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. given that some strains of f. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (ibd) with strains isolated from healthy controls to determine 1) whether this species was more commonly associated with ibd patients; and 2) whether gut-derived f. nucleatum strains from ibd pa ...	2011	21234912
evaluation of three swab transport systems for the maintenance of clinically important bacteria in simulated mono- and polymicrobial samples.	in this study, three swab transport systems were evaluated: m40 transystem, amies broth with a relatively new type of swab (both copan diagnostics, corona, ca, usa), and ssi transportmedium (statens serum institut, copenhagen denmark). the clsi m40-a standard procedures and 11 culture collection strains were used. the transport systems were tested at room temperature for holding times of 0, 24, and 48 h, and both mono- and polymicrobial samples were included. after 24 h of simulated transportati ...	2011	21284737
effect of ozone on periodontopathogenic species-an in vitro study.	the in vitro study was aimed to determine the effect of ozone on periodontopathogenic microorganisms. ozone was generated for 6 s-2?×?24 s (corresponding to 0.56 mg-2?×?2.24 mg of ozone) against 23 mainly anaerobic periodontopathogenic species. agar diffusion test was used as a screening method. then, the killing activity was tested in a serum-free environment and with 25% v/v inactivated serum. further, the effect of ozone on bactericidal activity of native serum was analyzed against fusobacter ...	2011	21287208
assessing the efficacy of vesicle fusion with planar membrane arrays using a mitochondrial porin as reporter.	reconstitution of functionally active membrane protein into artificially made lipid bilayers is a challenge that must be overcome to create a membrane-based biomimetic sensor and separation device. in this study we address the efficacy of proteoliposome fusion with planar membrane arrays. we establish a protein incorporation efficacy assay using the major non-specific porin of fusobacterium nucleatum (foma) as reporter. we use electrical conductance measurements and fluorescence microscopy to ch ...	2011	21295545
impact of systemic antimicrobials combined with anti-infective mechanical debridement on the microbiota of generalized aggressive periodontitis: a 6-month rct.	to compare the effects of systemic amoxicillin (amx) plus metronidazole (met) or placebos combined with anti-infective mechanical debridement on the sub-gingival microbiota of generalized aggressive periodontitis (gap).	2011	21303403
the effect of xylitol on the composition of the oral flora: a pilot study.	our aim was to investigate the effect of short-term xylitol consumption on the microbial composition of plaque and saliva.	2011	21311610
the origin of fusobacterium nucleatum involved in intra-amniotic infection and preterm birth.	objective. to evaluate the potential oral origin of fusobacterium nucleatum found in amniotic fluid of women at high risk of preterm birth. methods. a transversal study nested into a cohort study of women with preterm labor and/or preterm premature rupture of membranes was undergone. women with the presence of f. nucleatum in the amniotic fluid and their respective partners were invited to be examined for their periodontal health after delivery, and samples of saliva and subgingival plaque were ...	2011	21314291
the role of acidification in the inhibition of neisseria gonorrhoeae by vaginal lactobacilli during anaerobic growth.	vaginal lactobacilli protect the female genital tract by producing lactic acid, bacteriocins, hydrogen peroxide or a local immune response. in bacterial vaginosis, normal lactobacilli are replaced by an anaerobic flora and this may increase susceptibility to neisseria gonorrhoeae, a facultative anaerobe. bacterial interference between vaginal lactobacilli and n. gonorrhoeae has not been studied in liquid medium under anaerobic conditions. by co-cultivating n. gonorrhoeae in the presence of lacto ...	2011	21329492
short- and medium-chain fatty acids exhibit antimicrobial activity for oral microorganisms.	this study assessed the antibacterial activity of short-, medium-, and long-chain fatty acids against various oral microorganisms.	2011	21333271
epigenetic regulation of human β-defensin 2 and cc chemokine ligand 20 expression in gingival epithelial cells in response to oral bacteria.	gingival epithelia utilize multiple signaling pathways to regulate innate immune responses to various oral bacteria, but little is understood about how these bacteria alter epithelial epigenetic status. in this study we report that dna methyltransferase (dnmt1) and histone deacetylase expression were decreased in gingival epithelial cells treated with oral pathogen porphyromonas gingivalis and nonpathogen fusobacterium nucleatum. pretreatment with trichostatin a and sodium butyrate, which increa ...	2011	21248725
in vitro antimicrobial activity of razupenem (smp-601, ptz601) against anaerobic bacteria.	we evaluated the in vitro antianaerobic activity of razupenem (smp-601, ptz601), a new parenterally administered carbapenem, against 70 reference strains and 323 clinical isolates. razupenem exhibited broad-spectrum activity against anaerobes, inhibiting most of the reference strains when used at a concentration of =1 µg/ml. furthermore, it exhibited strong activity, comparable to those of other carbapenems (meropenem and doripenem), against clinically isolated non-fragilis bacteroides spp. (mic ...	2011	21343447
association between periodontal pathogens and risk of nonfatal myocardial infarction.	the direct effect of periodontal pathogens on atherosclerotic plaque development has been suggested as a potential mechanism for the observed association between periodontal disease and coronary heart disease, but few studies have tested this theory.	2011	21375559
rna-oligonucleotide quantification technique (roqt) for the enumeration of uncultivated bacterial species in subgingival biofilms.	approximately 35% of the species present in subgingival biofilms are as yet uncultivated, so their role in periodontal pathogenesis is unknown. the aim of the present study was to develop a high throughput method to quantify a wide range of cultivated and uncultivated taxa in subgingival biofilm samples associated with periodontal disease or health. oligonucleotides targeting the 16s ribosomal dna gene were designed, synthesized and labeled with digoxigenin. these probes were hybridized with the ...	2011	21375703
streptococcus cristatus attenuates fusobacterium nucleatum-induced cytokine expression by influencing pathways converging on nuclear factor-?b.	we previously reported that streptococcus cristatus, an oral commensal, was able to downregulate the interleukin-8 (il-8) response to fusobacterium nucleatum, a putative oral pathogen in oral epithelial cells. the aim of this study was to extend the understanding of how s. cristatus regulates cytokine expression in oral epithelial cells on a broad basis, and investigate whether the modulation of a toll-like receptor (tlr) pathway was involved in this process. kb and tert-2 cells were co-cultured ...	2011	21375705
in-vivo-induced antigenic determinants of fusobacterium nucleatum subsp. nucleatum.	fusobacterium nucleatum plays a pivotal role in dental plaque biofilm formation and is known to be involved in chronic inflammatory systemic disease. however, limited knowledge of f. nucleatum genes expressed in vivo interferes with our understanding of pathogenesis. in this study, we identified f. nucleatum genes induced in vivo using in-vivo-induced antigen technology (iviat). among 30,000 recombinant clones screened, 87 reacted reproducibly with pooled sera from 10 patients with periodontitis ...	2011	21375706
clinical spectrum of bacteraemic fusobacterium infections: from septic shock to nosocomial bacteraemia.	fusobacterium species are anaerobic bacteria that relatively rarely cause sepsis with a variable clinical presentation.	2011	21391770
clinical periodontal and microbiologic parameters in patients with rheumatoid arthritis.	background: a limited number of studies suggest the prevalence of periodontal pathogens in patients with rheumatoid arthritis (ra); however, results are inconsistent. the aim of this study was to investigate clinical periodontal and microbiologic parameters in patients with ra. methods: 66 patients with ra aged 49.5±8.4 years participated in the study. the periodontal situation was assessed with the periodontal screening index (psr(®)/psi); allocated to: healthy, gingivitis (psr(®)/psi score 0-2 ...	2011	21405936
clustering of subgingival microbial species in adolescents with periodontitis.	it has become increasingly recognized that groups of microorganisms interact within the subgingival plaque of adult subjects with periodontitis. it is much less clear, however, whether the consortia of microorganisms associated with periodontitis are different in early and more advanced cases of periodontitis. to investigate this point further, subgingival plaque was collected from six sites in 87 adolescents with periodontitis and 73 controls and the samples were analyzed for the detection of 1 ...	2011	21410554
four cases of bacteraemia caused by fusobacterium nucleatum in febrile, neutropenic patients.	although bacteraemia caused by obligate anaerobic bacteria is a rare event, this phenomenon will be an emerging problem among oncohaematological patients. we report four cases of bacteraemia caused by fusobacterium nucleatum in febrile, neutropenic patients over a 10 month period. all patients had haematological malignancy and severe neutropenia, and three of them suffered from oral mucositis or oedema of the oral mucosal surfaces, which was the probable portal of entry. all isolated strains wer ...	2011	21415204
microbiologic results after non-surgical erbium-doped:yttrium, aluminum, and garnet laser or air-abrasive treatment of peri-implantitis: a randomized clinical trial.	background: the purpose of this study is to assess clinical and microbiologic effects of the non-surgical treatment of peri-implantitis lesions using either an erbium-doped:yttrium, aluminum, and garnet (er:yag) laser or an air-abrasive subgingival polishing method. methods: in a 6-month clinical trial, 42 patients with peri-implantitis were treated at one time with an er:yag laser or an air-abrasive device. routine clinical methods were used to monitor clinical conditions. baseline and 6-month ...	2011	21417591
novel model for multispecies biofilms that uses rigid gas-permeable lenses.	oral biofilms comprise complex multispecies consortia aided by specific inter- and intraspecies interactions occurring among commensals and pathogenic bacterial species. oral biofilms are primary initiating factors of periodontal disease, although complex multifactorial biological influences, including host cell responses, contribute to the individual outcome of the disease. to provide a system to study initial stages of interaction between oral biofilms and the host cells that contribute to the ...	2011	21421785
structural basis of cooperative ligand binding by the glycine riboswitch.	the glycine riboswitch regulates gene expression through the cooperative recognition of its amino acid ligand by a tandem pair of aptamers. a 3.6 +à crystal structure of the tandem riboswitch from the glycine permease operon of fusobacterium nucleatum reveals the glycine binding sites and an extensive network of interactions, largely mediated by asymmetric a-minor contacts, that serve to communicate ligand binding status between the aptamers. these interactions provide a structural basis for how ...	2011	21439473
development of reflective biosensor using fabrication of functionalized photonic nanocrystals.	photonic crystals (pcs) are periodic dielectric structures that have a band-gap that forbids propagation of a certain range of wavelengths of light. this property enables control of light with remarkable facility by modification of the band-gaps and produce effects that are impossible with conventional optics. using chemically functionalized pcs, where the chemical functional group consists of amine and carboxyl group, in conjunction with a biomolecular probe material, the detection of pathogens ...	2011	21446513
mpc-polymer reduces adherence and biofilm formation by oral bacteria.	oral biofilms such as dental plaque cause dental caries and periodontitis, as well as aspiration pneumonia and infectious endocarditis by translocation. hence, the suppression of oral biofilm formation is an issue of considerable importance. mechanical removal, disinfectants, inhibition of polysaccharide formation, and artificial sugar have been used for the reduction of oral biofilm. from the viewpoint of the inhibition of bacterial adherence, we investigated whether aqueous biocompatible 2-met ...	2011	21447697
detection of periodontal bacteria in atheromatous plaques by nested polymerase chain reaction.	background. in recent years, increasing evidence regarding the potential association between periodontal diseases and cardiovascular diseases has been elicited. the available evidence underlines the importance of detecting periodontal pathogens on atheromatous plaques as the first step in demonstrating the causal relationship between both entities. it is the main aim of this investigation to detect periodontitis-associated bacteria from carotid artery atheromatous plaques recovered from patients ...	2011	21453047
the oral commensal, streptococcus gordonii, synergizes with tat protein to induce hiv-1 promoter activation in monocytes/macrophages.	trans-activator of transcription (tat) is an hiv-1 protein essential for viral replication. oral periodontopathogens (e.g. fusobacterium nucleatum) enhance hiv-1ltr promoter activation in monocytes/macrophages in absence of tat; however, some oral commensals fail to trigger this response. we sought to determine the effect of tat on hiv-1ltr promoter activation induced by the representative oral commensal streptococcus gordonii in monocytes/macrophages. s. gordonii enhanced hiv-1ltr reactivation ...	2011	21459369
multispecies communities: interspecies interactions influence growth on saliva as sole nutritional source.	human oral bacteria live in multispecies communities in the biofilm called dental plaque. this review focuses on the interactions of seven species and the ability of each species individually and together with other species to grow on saliva as the sole source of nutrient. community formation in biofilms in flow cells is monitored using species-specific fluorophore-conjugated immunoglobulin g, and images are captured by confocal microscopy. early colonizing veillonellae emerge from this review o ...	2011	21485308
analysis of interspecies adherence of oral bacteria using a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis profiling.	information on co-adherence of different oral bacterial species is important for understanding interspecies interactions within oral microbial community. current knowledge on this topic is heavily based on pariwise coaggregation of known, cultivable species. in this study, we employed a membrane binding assay coupled with polymerase chain reaction-denaturing gradient gel electrophoresis (pcr-dgge) to systematically analyze the co-adherence profiles of oral bacterial species, and achieved a more ...	2011	21485313
microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease.	uzel ng, teles fr, teles rp, song xq, torresyap g, socransky ss, haffajee ad. microbial shifts during dental biofilm re-development in the absence of oral hygiene in periodontal health and disease. j clin peridontol 2011; doi: 10.1111/j.1600-051x.2011.01730.x. abstract: aim: to monitor microbial shifts during dental biofilm re-development. materials and methods: supra- and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects at baseline and i ...	2011	21488936
influence of topography and hydrophilicity on initial oral biofilm formation on microstructured titanium surfaces in vitro.	objectives: the aim of this study was to analyse the influence of the microtopography and hydrophilicity of titanium (ti) substrates on initial oral biofilm formation. materials and methods: nine bacterial species belonging to the normal oral microbiota, including: aggregatibacter actinomycetemcomitans, actinomyces israelii, campylobacter rectus, eikenella corrodens, fusobacterium nucleatum, parvimonas micra, porphyromonas gingivalis, prevotella intermedia, and streptococcus sanguinis were teste ...	2011	21492236
identification and enzymatic analysis of a novel protein associated with production of hydrogen sulfide and l-serine from l-cysteine in fusobacterium nucleatum subsp. nucleatum atcc 25586.	a third enzyme that produced hydrogen sulfide from l-cysteine was identified in fusobacterium nucleatum subsp. nucleatum. the fn1055 gene was cloned from a cosmid library constructed with the genomic dna of f. nucleatum atcc 25586. despite the database annotation that the product of fn1055 is a cysteine synthase, reverse phase hplc revealed that no l-cysteine was produced in vitro by the purified fn1055 protein; however, the enzyme did produce l-serine. in addition, a cysteine auxotroph, escheri ...	2011	21493682
antigenicity of primary endodontic infection against macrophages by the levels of pge(2) production.	root canal contents are potent stimuli for proinflammatory cytokines involved in apical periodontitis. this study investigated target gram-negative bacterial species and endotoxins in primary endodontic infection with apical periodontitis, determined their antigenicity against macrophages through the levels of pge(2), and evaluated their relationship with clinical findings.	2011	21496656
pulmonary actinomycosis in a male patient with a tracheal bronchus.	<span style="font-weight: bold">	2011	21499592
sitafloxacin: in bacterial infections.	sitafloxacin is a fluoroquinolone antibacterial with in vitro activity against a broad range of gram-positive and -negative bacteria, including anaerobic bacteria, as well as against atypical pathogens. it is approved in japan for use in a number of bacterial infections caused by sitafloxacin-susceptible strains of staphylococcus spp., streptococcus pneumoniae, other streptococcus spp., enterococcus spp., moraxella catarrhalis, escherichia coli, citrobacter spp., klebsiella spp., enterobacter sp ...	2011	21504249
streptococcus cristatus modulates the fusobacterium nucleatum-induced epithelial interleukin-8 response through the nuclear factor-kappa b pathway.	zhang g, chen r, rudney jd.streptococcus cristatusmodulates thefusobacterium nucleatum-induced epithelial interleukin-8 response through the nuclear factor-kappa b pathway. j periodont res 2011; doi: 10.1111/j.1600-0765.2011.01373.x.©2011 john wiley & sons a/s background and objective: we previously reported that the interleukin-8 (il-8) response to fusobacterum nucleatum was attenuated in the presence of streptococcus cristatus. here, we further examined the underlying mechanism(s) involved in ...	2011	21521225
spiramycin resistance in human periodontitis microbiota.	purpose: the occurrence of in vitro resistance to therapeutic concentrations of spiramycin, amoxicillin, and metronidazole was determined for putative periodontal pathogens isolated in the united states. materials and methods: subgingival plaque specimens from 37 consecutive adults with untreated severe periodontitis were anaerobically cultured, and isolated putative periodontal pathogens were identified to a species level. in vitro resistance to spiramycin at 4 μg/ml, amoxicillin at 8 μg/ml, an ...	2011	21524712
fusobacterium nucleatum infection of colonic cells stimulates muc2 mucin and tumor necrosis factor-{alpha}	even though the etiology of inflammatory bowel disease is not completely known, it is influenced by the presence of normal gut microflora as well as-yet, unrecognized pathogens. the anaerobic, gram-negative bacterial species, fusobacterium nucleatum, is a common resident of the human mouth and gut and varies in its pathogenic potential. in this study, we demonstrate that highly invasive f. nucleatum isolates derived from the inflamed gut of crohn's disease patients evoked significantly greater m ...	2011	21536792
inhibitory effect of lactobacillus reuteri on periodontopathic and cariogenic bacteria.	the interaction between lactobacillus reuteri, a probiotic bacterium, and oral pathogenic bacteria have not been studied adequately. this study examined the effects of l. reuteri on the proliferation of periodontopathic bacteria including aggregatibacter actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, and tannerella forsythia, and on the formation of streptococcus mutans biofilms. human-derived l. reuteri strains (kctc 3594 and kctc 3678) and rat-derived l. reuteri kctc ...	2011	21538238
interaction of oral bacteria with gingival epithelial cell multilayers.	primary gingival epithelial cells were cultured in multilayers as a model for the study of interactions with oral bacteria associated with health and periodontal disease. multilayers maintained at an air-liquid interface in low-calcium medium displayed differentiation and cytokeratin properties characteristic of junctional epithelium. multilayers were infected with fluorescently labeled porphyromonas gingivalis, aggregatibacter actinomycetemcomitans, fusobacterium nucleatum or streptococcus gord ...	2011	21545698
antibacterial compounds from siraitia grosvenorii leaves.	luo han guo (lhg) fruits (siraitia grosvenorii swingle) have been used as traditional medicine in china for centuries to treat sore throats and coughs. however, lhg leaves are seldom used and minimal scientific information is available on them. in our recent study on the leaves of s. grosvenorii, the bioactive compounds β-amyrin (2), aloe emodin (5), aloe-emodin acetate (6), 5α,8α-epidioxy-24(r)-methylcholesta-6,22-dien-3β-ol (7) and p-hydroxyl benzyl acid (8), accompanied by n-hexadecaoic acid ...	2011	21547839
garlic allicin as a potential agent for controlling oral pathogens.	abstract garlic has been used medicinally throughout human history. allicin is considered the most therapeutic constituent of garlic. this study tested the antimicrobial activity of garlic allicin on oral pathogens associated with dental caries and periodontitis. allicin was found effective against all the tested bacteria. the broth dilution method revealed that planktonic growth of the cariogenic, gram-positive species streptococcus mutans, s. sobrinus, and actinomyces oris was inhibited by an ...	2011	21548800
oxidative stress response in the opportunistic oral pathogen fusobacterium nucleatum.	the anaerobic, gram-negative bacillus fusobacterium nucleatum plays a vital role in oral biofilm formation and the development of periodontal disease. the organism plays a central bridging role between early and late colonizers within dental plaque and plays a protective role against reactive oxygen species. using a two-dimensional gel electrophoresis and mass spectrometry approach, we have annotated 78 proteins within the proteome of f. nucleatum subsp. nucleatum and identified those proteins w ...	2011	21563313
antimicrobial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study.	becerik s, türkoğlu o, emingil g, vural c, özdemir g, atilla g. anti-microbial effect of adjunctive use of chlorhexidine mouthrinse in untreated gingivitis: a randomized, placebo-controlled study. apmis 2011; 119: 364-72. the aim of this study was to examine the effectiveness of chlorhexidine mouthrinse (chx) in addition to daily plaque control on subgingival microbiota in patients with untreated gingivitis. fifty gingivitis patients were randomized to chx or placebo groups. chx group rinsed wit ...	2011	21569094
experimental alveolitis in rats: microbiological, acute phase response and histometric characterization of delayed alveolar healing.	the pathogenesis of alveolitis is not well known and therefore experimental situations that mimic some features of this disease should be developed.	2011	21625744
activation of the contact system at the surface of fusobacterium necrophorum: a possible virulence mechanism in lemierre's syndrome.	fusobacterium necrophorum causes lemièrre's syndrome, a serious disease with septic thrombophlebitis of the internal jugular vein, pulmonary involvement and systemic inflammation. the contact system is a link between inflammation and coagulation and contact activation by the bacteria could therefore contribute to the abnormal coagulation and inflammation seen in patients with lemièrre's syndrome. in this study, f. necrophorum was found to bind radiolabeled high molecular weight kininogen (hk), a ...	2011	21646449
case records of the massachusetts general hospital. case 1-2011. a 35-year-old man with fever, bacteremia, and a mass in the left atrium.		2011	21226582
fusobacterium necrophorum endocarditis case report and review of the literature.	fusobacterium necrophorum is a gram-negative anaerobic bacillus that can cause serious systemic infections typically in previously healthy young adults. lemierre's syndrome, also known as post-anginal sepsis or necrobacillosis, is the infection most usually associated with f. necrophorum. however, f. necrophorum is also a very rare cause of anaerobic endocarditis. mortality and rates of thromboembolism are high with f. necrophorum endocarditis. in this article, we describe a case of f. necrophor ...	2011	21251564
a 17-year-old adolescent with persistent sore throat.		2011	21323201
identification of an l-methionine {gamma}-lyase involved in the production of hydrogen sulfide from l-cysteine in fusobacterium nucleatum subsp. nucleatum atcc 25586.	fusobacterium nucleatum produces an abundance of hydrogen sulfide (h(2)s) in the oral cavity, which is mediated by several enzymes. the identification and characterization of three distinct enzymes (fn0625, fn1055 and fn1220) in f. nucleatum that catalyze the production of h(2)s from l-cysteine has been reported. in the current study, a novel enzyme involved in the production of h(2)s in f. nucleatum atcc 25586, whose molecular mass had been estimated to be approximately 130-kda, was identified ...	2011	21798982
necrobacillosis in humans.	necrobacillosis, often used synonymously with lemierre's syndrome, is a form of abscess infection in the peritonsillar area associated with a thrombophlebitis and caused by the strict anaerobic species fusobacterium necrophorum. the thrombosis formed affects the internal jugular vein, from which the bacteria are seeded out in the bloodstream and cause bacteremia. septicemia is a common complication with an often fatal outcome. necrobacillosis is very rare and is referred to as the 'forgotten dis ...	2011	21342070
microbiological aspects of human mandibular subperiosteal dental implants.	abstract purpose: clinical, microbiological and biochemical features of human mandibular subperiosteal dental implants exhibiting peri-implantitis were compared to those experiencing long-term peri-implant health.materials and methods: after evaluation of clinical parameters, submucosal plaque biofilm samples were obtained from permucosal implant abutment posts exhibiting probing depths = 5 mm and bleeding on probing in subjects with peri-implantitis (n = 3), and from posts with peri-implant hea ...	2011	21767207
a 25-year-old male with oligoarthritis as the first sign of lemierre's syndrome.		2011	21221703
analysis of the antimicrobial susceptibility of anaerobic bacteria isolated from endodontic infections in brazil during a period of nine years.	the purpose of this study was to analyze the susceptibility of some anaerobic species isolated from a brazilian population at different periods of time by determining a pattern of development of resistance to frequently prescribed antibiotics in endodontics.	2011	21763894
analysis of the lung microbiome in the "healthy" smoker and in copd.	although culture-independent techniques have shown that the lungs are not sterile, little is known about the lung microbiome in chronic obstructive pulmonary disease (copd). we used pyrosequencing of 16s amplicons to analyze the lung microbiome in two ways: first, using bronchoalveolar lavage (bal) to sample the distal bronchi and air-spaces; and second, by examining multiple discrete tissue sites in the lungs of six subjects removed at the time of transplantation. we performed bal on three neve ...	2011	21364979
molecular sensing by the aptamer domain of the fmn riboswitch: a general model for ligand binding by conformational selection.	understanding the nature of the free state of riboswitch aptamers is important for illuminating common themes in gene regulation by riboswitches. prior evidence indicated the flavin mononucleotide (fmn)-binding riboswitch aptamer adopted a 'bound-like' structure in absence of fmn, suggesting only local conformational changes upon ligand binding. in the scope of pinpointing the general nature of such changes at the nucleotide level, we performed shape mapping experiments using the aptamer domain ...	2011	21745821
a novel organotypic dento-epithelial culture model: effect of fusobacterium nucleatum biofilm on b-defensin-2, -3, and ll-37 expression.	background: in the present study, the expression and localization of three epithelial peptides, human +¦-defensin (hbd)-2, -3, and cathelicidin (ll-37), were studied in an organotypic dento-epithelial (od-e) model exposed to fusobacterium nucleatum biofilm. methods: biofilm of f. nucleatum atcc 25586 or ahn 9508 were produced by culturing each strain on semipermeable membranes. the od-e model was constructed by seeding keratinocytes on fibroblast-containing collagen gels and by placing dentin pi ...	2011	21692631
invasive potential of gut mucosa-derived fusobacterium nucleatum positively correlates with ibd status of the host.	fusobacterium nucleatum is a heterogeneous oral pathogen that is also a common resident of the human gut mucosa. given that some strains of f. nucleatum are known to be invasive and proinflammatory in the oral mucosa, we compared strains isolated from patients with inflammatory bowel disease (ibd) with strains isolated from healthy controls to determine 1) whether this species was more commonly associated with ibd patients; and 2) whether gut-derived f. nucleatum strains from ibd patients showed ...	2011	21830275
epidemiological characteristics of infections caused by bacteroides, prevotella and fusobacterium species: a prospective observational study.	in order to investigate differences among infections due to gram-negative anaerobic bacteria (bacteroides, prevotella and fusobacterium spp.), clinical, epidemiological, and microbiological data were collected and evaluated from 206 anaerobic infections. the most frequently isolated species was bacteroides fragilis. the majority of the cases were intra-abdominal infections (49%) followed by skin and soft tissue infections (24.7%). logistic regression analysis showed that bacteroides spp. strains ...	2011	21664284
[a young man with mononucleosis and swelling of the right eye].	treatment of acute infections in the upper airways comprises a significant part of direct healthcare expenditure and is a challenge for healthcare professionals. in norway, 11,495 hospitalized days were recorded for acute infections (influenza and pneumonia excluded) in the upper airways in 2008. acute bacterial rhinosinusitis (abrs) is defined as inflammation of the nose and the paranasal sinuses characterized by both 1): symptoms of nasal blockage/obstruction/congestion or nasal discharge, and ...	2011	21233887
efficacy of chlorhexidine digluconate-containing formulations and other mouthrinses against periodontopathogenic microorganisms.	objective: to determine in vitro the action of chlorhexidine digluconate and different commercially available mouthrinses on oral microorganisms. method and materials: minimal inhibitory concentrations and possible induction of resistance by chlorhexidine digluconate, an essential oil-containing mouthwash and an amine fluoride/stannous fluoride solution, were determined against microorganisms normally found in the oral cavity (10 streptococci, 2 enterobacteria, 1 candida albicans, 8 porphyromona ...	2011	21842009
the identification of anaerobic bacteria using maldi-tof ms.	matrix assisted laser desorption and ionization time-of-flight mass spectrometry (maldi-tof ms) has gained more and more popularity for the identification of bacteria. several studies show that bacterial diagnosticis is being revolutionized by the application of maldi-tof ms. for anaerobic bacteria, maldi-tof ms has been used for the identification of prevotella spp., fusobacterium spp., clostridium spp., bacteroides spp. and gram-positive anaerobic cocci. however, to identify bacteria reliably, ...	2011	21515395
[prevalence of helicobacter pylori detected by real-time pcr in the subgingival plaque of patients with chronic periodontitis].	helicobacter pylori plays a significant role in gastric disease. the aim of the present pilot study was to evaluate the prevalence of h. pylori detected by real-time polymerase chain reaction (real-time pcr) in the sub-gingival plaque of chronic periodontitis patients and to determine periodontopathogens profile of positive sites at h. pylori.	2011	21688602
recovery of aerobic and anaerobic bacteria in sinus fungal ball.	this case study with chart review describes the aerobic and anaerobic microbiology of specimens obtained from 47 patients with aspergillus spp fungus ball. bacteria were recovered from 32 of the 47 (68%) patients. eighty-six isolates, 29 aerobic and facultatives and 57 anaerobic, were recovered. aerobic and facultatives only were recovered in 6 instances (19% of culture-positive specimens), anaerobes only in 11 (34%), and mixed aerobes and anaerobes in 15 (47%). the predominant aerobes were stap ...	2011	21804027
an unusual infection in an injecting drug user.	injecting drug users are prone to atypical infections. we present a case of septic thrombophlebitis secondary to fusobacterium gonidiaformans infection in a heroin user, which demonstrates the frequently unusual nature of pathogens and presentations in this group of patients.	2011	21515536
acute appendicitis is characterised by local invasion with fusobacterium nucleatum/necrophorum.	acute appendicitis is a local intestinal inflammation with unclear origin. the aim was to test whether bacteria in appendicitis differ in composition to bacteria found in caecal biopsies from healthy and disease controls.	2011	19926616
antiaggregation potential of berry fractions against pairs of streptococcus mutans with fusobacterium nucleatum or actinomyces naeslundii.	coaggregation is an interspecies adhesion process, which is essential to the development of dental plaque. this is an in vitro study of the composition of the soluble solids in the berry juice molecular size fractions (<10 kda, fi; 10-100 kda, fii; >100 kda, fiii) derived from apple, bilberry, blackcurrant, cloudberry, crowberry and lingonberry and their ability to inhibit and reverse coaggregation of the pairs of common species in dental plaque: streptococcus mutans with fusobacterium nucleatum ...	2011	20623601
fusobacterium nucleatum regulation of neutrophil transcription.	abnormal neutrophil responses have been observed in periodontitis patients, including hyper-reactivity in terms of production of reactive oxygen species (ros) following exposure to the key quorum-sensing plaque bacterium, fusobacterium nucleatum. this study was designed to characterize the transcriptional response of neutrophils to f. nucleatum.	2011	20663022
microbial communities in the tonsils of healthy pigs.	the tonsils of mammals such as humans and pigs are colonized with an extensive microbiota and are frequently the site for asymptomatic carriage of bacterial pathogens. the goal of this study was to determine the composition of the microbial community of the tonsils in healthy pigs. tonsils were collected from eight pigs from two different healthy herds. samples of the tonsils from each pig were used for culture dependent and culture independent identification of the microbial community. aerobic ...	2011	20663617
polybacterial challenge effects on cytokine/chemokine production by macrophages and dendritic cells.	to investigate the polymicrobial infection of periodontal disease, which elicits inflammatory mediators/cytokines/chemokines in the local gingival tissues, and a polybacterial challenge of antigen-presenting cells, e.g. macrophages and dendritic cells (dcs), at the mucosal surface.	2011	20798974
[brain abscess as the first clinical manifestation of isolated pulmonary arteriovenous malformation without rendu-osler disease].	brain abscesses occur in 5 to 13 % of patients with pulmonary arteriovenous malformation (pavm), more often present in rendu-osler-weber disease or hereditary hemorrhagic telangiectasia (hht).	2011	20466398
fusobacterium nucleatum binding to complement regulatory protein cd46 modulates the expression and secretion of cytokines and matrix metalloproteinases by oral epithelial cells.	periodontitis is a chronic inflammatory disease that results in the destruction of the supporting tissues of the teeth. gingival epithelial cells are an important mechanical barrier and participate in the host inflammatory response to periodontopathogens. the aim of the present study is to investigate the capacity of fusobacterium nucleatum to bind to the complement regulatory protein cd46 expressed by oral epithelial cells and to determine the impact of the binding on the gene expression and pr ...	2011	20843232
a 6-month study of the effects of 0.3% triclosan/copolymer dentifrice on dental implants.	supportive therapy to maintain dental implants is increasingly important. this study examined the effect of a 0.3% triclosan/2% copolymer dentifrice on oral biofilms and gingival inflammation (gi) on dental implants and peri-implant tissues.	2011	20831669
lemierre's syndrome.	lemierre's syndrome is a rare disease that results in an oropharyngeal infection, which precipitates an internal jugular vein thrombosis and metastatic infection. fusobacterium necrophorum is an anaerobic gram-negative bacillus and has been identified as the causative agent. we describe the case of a young girl whose presentation and diagnosis were confounded by a history of valvular heart disease. infection of heart valves can produce many of the signs and symptoms associated with lemierre's sy ...	2011	19002548
rapid quantification of periodontitis-related bacteria using a novel modification of invader plus technologies.	the invader plus technology is a sensitive, rapid method for the detection and quantification of nucleic acid. while the original technology is based on the amplification by polymerase chain reaction (pcr) of the target sequence followed by its detection using the invader technology, the current modification allows simultaneous pcr amplification and invader reaction. the pcr primers and the invader probes are designed to operate at the same temperature. this allows simpler design and faster resu ...	2010	18718748
oropharyngeal infection with metastatic hand infection: an uncommon variant of lemierre's syndrome.	lemierre's syndrome is caused by acute oropharyngeal infection with septic secondary thrombophlebitis of the internal jugular vein and metastatic infection. fusobacterium necrophorum is the most commonly implicated aetiological agent. the authors present the case of a healthy young man presenting with a history of general malaise and sore throat who developed septic metastatic complications in the left upper limb. he made a complete recovery after treatment with a combination of parenteral antib ...	2010	20527653
energy dose parameters affect antimicrobial photodynamic therapy-mediated eradication of periopathogenic biofilm and planktonic cultures.	this study evaluated the in vitro efficacy of a commercially available apdt system in eradication of the periopathogens porphyromonas gingivalis, fusobacterium nucleatum, and aggregatibacter actinomycetemcomitans in both planktonic and biofilm cultures.	2010	20666581
vaccination of mice with porphyromonas gingivalis or fusobacterium nucleatum modulates the inflammatory response, but fails to prevent experimental periodontitis.	to assess the potential of using vaccination with porphyromonas gingivalis or fusobacterium nucleatum, in modulating local subcutaneous inflammatory response and alveolar bone loss following coinfection with both bacteria.	2010	20670341
lemierre syndrome caused by co-infection by arcanobacterium haemolyticum and fusobacterium necrophorum.		2010	20661621
lemierre-like syndrome: a series of unique cases.		2010	20488807
[identification of anaerobic bacteria isolated from various clinical specimens and determination of antibiotic susceptibilities].	routine isolation, identification and susceptibility testing of anaerobic bacteria present several difficulties leading to defects in the determination of local susceptibility patterns which will guide empirical treatment protocols. this study was carried out to identify the anaerobic bacteria isolated from various clinical materials obtained from the suspected patients with anaerobic infection and to determine the antibiotic susceptibilities against several antibiotics. one hundred clinical spe ...	2010	20549955
fusobacterium nucleatum-associated beta-defensin inducer (fad-i): identification, isolation, and functional evaluation.	human β-defensins (hbds) are small, cationic antimicrobial peptides, secreted by mucosal epithelial cells that regulate adaptive immune functions. we previously reported that fusobacterium nucleatum, a ubiquitous gram-negative bacterium of the human oral cavity, induces human β-defensin 2 (hbd2) upon contact with primary oral epithelial cells. we now report the isolation and characterization of an f. nucleatum (atcc 25586)-associated defensin inducer (fad-i). biochemical approaches revealed a ce ...	2010	20847052