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agoraphobia and parental bereavement.the prevalence of parental bereavement was determined in 50 married female outpatients with a dsm-iii diagnosis of agoraphobia and in a control group of married female outpatients diagnosed as having non-psychotic psychiatric disorders other than agoraphobia. the two groups were matched for age and overall severity of psychiatric symptoms. compared with the general population, the patient control group reported a statistically significant excess of parental, but not maternal, bereavement. the ag ...20123435374
thyroglobulin and 131i uptake of remaining tissue in patients with differentiated carcinoma after thyroidectomy.in 158 thyroidectomized patients with well-differentiated non-metastatic thyroid cancer the results of serum thyroglobulin (tg) determination and 131i uptake values established immediately before radioiodine treatment (19 days after surgery) were compared. in 113 patients (72%) tg was above 6 ng ml-1 (lower limit of detection) and 131i uptake exceeded 2%. in 11 patients (7%) tg was undetectable and 131i uptake less than 2%. in these cases a radioiodine treatment was not performed; the thyroid ab ...20123399222
evaluation of aerosolized triamcinolone acetonide as a replacement for aerosolized beclomethasone dipropionate.substitution of aerosolized triamcinolone acetonide for aerosolized beclomethasone dipropionate in treatment of 30 chronic asthmatics resulted in subjective improvement in all patients. there was reduction in requirement of systemic steroids and inhaled beta adrenergics. no adrenal suppression or oropharyngeal candidiasis occurred after replacing beclomethasone dipropionate with triamcinolone acetonide.20123528120
annual summary 1984. reported morbidity and mortality in the united states. 20113092006
[x-ray computed tomography in venous pathology].after presenting the tomodensitometric semiology of venous thromboses in various territories, the authors define its indications. it is essentially in deep territories, head, chest and abdomen that the ct-scan is used to study and diagnose unrecognized thromboses as well as determine the venous repercussion of various neighboring ailments. the respective place of the ct-scan, ultrasonography and various phlebographies is analyzed.20113043474
synthesis and antineoplastic effects of furo[3,4-d]pyrimidine derivatives.furo[3,4-d]pyrimidine was obtained in the reaction of 2-phenyl-4-phenylamino-6-methyl-5-pyrimidinecarboxylic acid with socl2. this compound, heated with aliphatic amines, yielded mono- and diamino-derivatives. some of the obtained compounds exhibited a potent antineoplastic activity.20113237569
oesophageal pain exacerbated by propranolol. 20113113570
valproate-associated hepatotoxicity and its biochemical mechanisms.intake of the anticonvulsant drug valproic acid, or its sodium salt, has been associated with occasional instances of severe and sometimes fatal hepatotoxicity. probably at least 80 cases have occurred worldwide. the syndrome affects perhaps 1 in 10,000 persons taking the drug, and usually develops in the early weeks or months of therapy. most instances have involved children, usually those receiving more than 1 anticonvulsant. multiple cases have occurred in 2 families. the typical presentation ...20113131628
[respiratory problems in patients with muscular dystrophy]. 20113285556
[identification of vibrio mimicus bacteriophages].lysogeny was studied in vibrio mimicus; the indicator v. cholerae el tor strain was selected to identify phages. new v. mimicus phages were obtained and identified, which had a morphological similarity and an antigen affinity for morphological group i cholerae phages. phage differentiation revealed that morphological group i v. mimicus phages showed certain differences manifested as their lytic activity against v. cholerae strain 1322-69 of serovar 37 while this property was absent in cholerae p ...201121574461
design and validation of a novel multiplex real-time pcr assay for vibrio pathogen detection.three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus--account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species. additionally, biochemical identification and confirmation require 2 or more days to complete. rapid and sensitive molecular techniques for the detection of vibrio pathogens would be useful for the surveillance and management o ...201121669071
genome sequencing reveals unique mutations in characteristic metabolic pathways and the transfer of virulence genes between v. mimicus and v. cholerae.vibrio mimicus, the species most similar to v. cholerae, is a microbe present in the natural environmental and sometimes causes diarrhea and internal infections in humans. it shows similar phenotypes to v. cholerae but differs in some biochemical characteristics. the molecular mechanisms underlying the differences in biochemical metabolism between v. mimicus and v. cholerae are currently unclear. several v. mimicus isolates have been found that carry cholera toxin genes (ctxab) and cause cholera ...201121731695
Detection and characterization of pathogenic vibrios in shellfish by a Ligation Detection Reaction-Universal Array approach.Vibrios are a group of major foodborne pathogens widely distributed in marine environment. Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus are the pathogenic species of Vibrio that pose the greatest threat to human health. However, other vibrios, e.g. Vibrio alginolyticus, Vibrio mimicus and Grimontia hollisae, apparently less relevant in the group of foodborne pathogens, have been sporadically found in outbreaks. For seafood safety and economic purposes, a rapid and powerful met ...201122177227
structural variation of the superintegron in the toxigenic vibrio cholerae o1 el tor.to understand the genetic structures and variations of the superintegron (si) in vibrio cholerae isolated in the seventh cholera pandemic.201122365393
physical and psychological sequelae to torture. a controlled clinical study of exiled asylum applicants.the study comprised 24 male lebanese refugees living in denmark. twelve of them alleged having been tortured in lebanon during the period 1981-85. the remaining twelve had neither been imprisoned nor tortured and thus acted as control persons. all the testimonies were found to be valid according to a method previously used by the author. the most common forms of torture were blows against the head, body and foot soles, suspension and asphyxiation. threats and solitary confinement were frequent, ...20113209147
increased cough reflex associated with angiotensin converting enzyme inhibitor cough. 20113120857
darkroom diseases and how to combat them. 20113112384
computer-aided molecular modelling: research study or research tool?developments in computational methods and equipment have produced a new type of research chemist, who prefers to calculate properties as well as measure them, either to gain a better understanding of microscopic molecular behaviour per se, or to guide a broader scientific study using a so-called 'rational' approach. while there is good reason to believe that significant results can be obtained this way, it is clear that only some of the 'tools of the trade' are sufficiently robust to present to ...20113236047
[enterostoma therapy--a pilot course in the essen education center of the german nursing association]. 20113127632
development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of the tdh and trh genes of vibrio parahaemolyticus and related vibrio species.thermostable direct hemolysin (tdh) and tdh-related hemolysin (trh) are the major virulence determinants of vibrio parahaemolyticus. trh is further differentiated into trh1 and trh2 on the basis of genetic and phenotypic differences. we developed a novel and highly specific loop-mediated isothermal amplification (lamp) assay for sensitive and rapid detection of the tdh, trh1, and trh2 genes of v. parahaemolyticus. the lamp assay was designed for both combined and individual detection of the tdh, ...201019966027
notes from the field: vibrio mimicus infection from consuming crayfish --- spokane, washington, june 2010.on june 24, 2010, the spokane (washington) regional health district (srhd) was notified of two hospitalized patients under intensive care with severe dehydration whose stool specimens yielded vibrio mimicus. cdc was asked to assist with the environmental and epidemiologic investigation. investigators learned that both persons had consumed crayfish on june 20, 2010. the previous day, live crayfish obtained from an online seafood company had been boiled and served warm at a party. the chef reporte ...201021030943
identification and characterization of a vibrio mimicus gene encoding the heme/hemoglobin receptor.the present authors have previously reported that vibrio mimicus expresses 77-kda and 80-kda outer membrane proteins in response to iron-limited conditions, and that the 77-kda protein serves as the receptor for ferriaerobactin. in this study, it was found that v. mimicus can use heme and hemoglobin as iron sources. furta was then applied to v. mimicus 7pt to obtain candidate gene fragments involved in utilization of heme and hemoglobin. one furta-positive clone was shown to contain a partial ge ...201021118298
renal growth after neonatal urinary tract infection.this study presents the result of 12-21 years' follow-up in a group of children with neonatal urinary tract infection (onset within 1 month after birth) in whom early renal growth retardation was noted without concomitant classical renal scarring. in all cases the neonatal infection was diagnosed and treated within a few days of onset and the patients were closely supervised thereafter. renal length, parenchymal thickness and area were measured at urography. at first follow-up (22 children, mean ...20103153287
[epidemiologic investigations on caries prevalence among schoolchildren in brittany (france)].1421 randomly selected 6- to 15-year-old breton children were examined in 1987 in order to assess the prevalence of caries in permanent teeth. methods proposed by turlot & cahen (1989) were used. at 6, 85.9% of all children were caries-free and at 15, only 5.3%. at 14, dmft and dmfs rates reached 6.46 and 12.36, respectively. the differences of scores stated between boys and girls and between children from urban and rural regions were not statistically significant. pits and fissures were more fr ...20102799351
interference of bromide in determination of serum chloride. 20103815810
the hypothalamic-pituitary-adrenal axis in depression. 20103035885
effects of blocked versus random practice by mentally retarded subjects on learning a novel skill.the purpose of this study was to assess the effects of different practice conditions on mentally retarded subjects learning a novel task. 20 subjects with a mean iq of 51.82 were randomly assigned to one of two practice conditions. the first group practiced at three different speeds (60, 30, and 15 rpm) on the pursuit rotor using a block arrangement in which each speed was practiced for five consecutive trials of 20 sec. duration before practicing at the next speed. the second group used random ...20102812988
development of a single base extension-tag microarray for the detection of pathogenic vibrio species in seafood.in this study, a single base extension-tag array on glass slides (sbe-tags) microarray was established to detect the seven leading seafood-borne pathogens, including vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio mimicus, vibrio alginolyticus, vibrio anguillarum, and vibrio harveyi. three multiplex pcr assays were developed to specifically target the following species with individual gene markers, which are aads, tdh, and trh for v. parahaemolyticus; col, toxr, and vvh for v ...201021161212
simultaneous differential detection of human pathogenic and nonpathogenic vibrio species using a multiplex pcr based on gyrb and pnta genes.to develop a multiplex pcr targeting the gyrb and pnta genes for vibrio species differentiation.201019891709
validation of a method for the detection of five species, serogroups, biotypes and virulence factors of vibrio by multiplex pcr in fish and seafood.in this work a sequential multiplex pcr system was designed and validated for the detection of most frequent foodborne pathogen vibrio species in fish and seafood (vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio alginoliticus and vibrio mimicus). the method proposed functions in a hierarchical way, being composed of an end-point multiplex pcr to detect the presence of dna belonging to the studied species, followed by multiplex pcr and fragment analysis allowing the viability ...201019913702
comparative genomics of clinical and environmental vibrio mimicus.whether vibrio mimicus is a variant of vibrio cholerae or a separate species has been the subject of taxonomic controversy. a genomic analysis was undertaken to resolve the issue. the genomes of v. mimicus mb451, a clinical isolate, and vm223, an environmental isolate, comprise ca. 4,347,971 and 4,313,453 bp and encode 3,802 and 3,290 orfs, respectively. as in other vibrios, chromosome i (c-i) predominantly contains genes necessary for growth and viability, whereas chromosome ii (c-ii) bears gen ...201021078967
presence of genes for type iii secretion system 2 in vibrio mimicus strains.vibrios, which include more than 100 species, are ubiquitous in marine and estuarine environments, and several of them e.g. vibrio cholerae, v. parahaemolyticus, v. vulnificus and v. mimicus, are pathogens for humans. pathogenic v. parahaemolyticus strains possess two sets of genes for type iii secretion system (t3ss), t3ss1 and t3ss2. the latter are critical for virulence of the organism and be classified into two distinct phylogroups, t3ss2α and t3ss2β, which are reportedly also found in patho ...201021110901
rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry.vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ...201020059616
occurrence of the vibrio cholerae seventh pandemic vsp-i island and a new variant.using comparative genomics, we identified a new variant of the vibrio seventh pandemic island-i (vsp-i). results of polymerase chain reaction (pcr) screening for both known vsp-i variants indicate that the novel variant is present only in non-o1/non-o139 strains of v. cholerae and vibrio mimicus. comparative genomics revealed little sequence divergence in the seventh pandemic vsp-i; however, a second insertion site located on the smaller chromosome was identified. although the seventh pandemic v ...201020141327
[simultaneous detection of vibrio cholerae o1 and o139 by real-time quantitative pcr].targeting the specific o-antigen gene cluster, a taq-man real-time fluorescence pcr assay was developed to detect 01 and o139 vibrio cholera concurrently and avoid frequently occurred false positives.201020364601
in vitro antibacterial, cytotoxic and antioxidant activities of plant nephelium longan.the petroleum ether, chloroform and ethyl acetate fractions of ethanol extract of leaf and stem from the plant nephelium longan (fam-sapindaceae) was subjected to antioxidant, antibacterial and cytotoxic activity. all the fractions showed potent antioxidant activity, of which the ethyl acetate and chloroform fraction of leaf demonstrated the strongest antioxidant activity with the ic50 value of 44.28 and 44.31 microg ml(-1), respectively. the petroleum ether extracts (500 microg disc(-1)) of lea ...201020415149
identification of non-coding rnas in environmental vibrios.the discovery of non-coding rna (ncrna) has been mainly limited to laboratory model systems and human pathogenic bacteria. in this study, we begin to explore the ncrna diversity in four recently sequenced environmental vibrio species (vibrio alginolyticus 40b, vibrio communis 1da3, vibrio mimicus vm573 and vibrio campbellii baa-1116) by performing in silico searches using infernal and rfam for the identification of putative ncrna-encoding genes. this search method resulted in the identification ...201020447992
interaction between vibrio mimicus and acanthamoeba castellanii.vibrio mimicus is a gram-negative bacterium, which causes gastroenteritis and is closely related to vibrio cholerae. the environmental reservoir of this bacterium is far from defined. acanthamoeba as well as vibrio species are found in diverse aquatic environments. the present study was aimed to investigate the ability of a. castellanii to host v. mimicus, the role of bacterial protease on interaction with a. castellanii and to disclose the ability of cysts to protect intracellular v. mimicus. c ...201020454692
discovery of novel vibrio cholerae vsp-ii genomic islands using comparative genomic analysis.this report describes vibrio seventh pandemic island ii (vsp-ii) and three novel variants revealed by comparative genomics of 23 vibrio cholerae strains and their presence among a large and diverse collection of v. cholerae isolates. three vsp-ii variants were reported previously and our results demonstrate the presence of three novel vsp-ii in clinical and environmental v. cholerae marked by major deletions and genetic rearrangements. a new vsp-ii cluster was found in the seventh pandemic v. ch ...201020528940
anticholinergic drugs in the treatment of airways disease. 20102861843
[biochemical aspect in cardiac ischemia--ischemic changes and reperfusion injury]. 20102838873
[70 years nursing school innsbruck]. 20092694061
[poisoning by geromitra esculenta].gyromitra esculenta (persoon ex fries) mushrooms have been responsible for severe intoxications and even deaths. clinical data are characterized primarily by vomiting and diarrhoea, and, afterwhile, by jaundice, convulsions and coma. the species of concern are mainly g. esculenta, g. fastigiata and g. gigas; nevertheless, recent advances in chromatography, biochemistry and toxicology have established that other species within the ascomycetes may prove also toxic. the toxins, i.e. gyromitrin (n-m ...20092681713
[the results of an investigation of the various factors influencing the late patency ratio of coronary bypass grafting].the purpose of this communication was to find out the factors influencing the late patency ratio of coronary artery bypass grafts of 42 patients in our institution. the following results were obtained: 1. the size of the measured diameters of coronary arteries and the graft flow rates were correlated well to the late patency ratio (p less than 0.05). 2. the regular physical exercise trainings were significantly important to keep patent grafts (p less than 0.01). 3. in blood serum studies, serum ...20092786538
dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus.the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ...200920735646
rna colony blot hybridization method for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria.a species-specific rna colony blot hybridization protocol was developed for enumeration of culturable vibrio cholerae and vibrio mimicus bacteria in environmental water samples. bacterial colonies on selective or nonselective plates were lysed by sodium dodecyl sulfate, and the lysates were immobilized on nylon membranes. a fluorescently labeled oligonucleotide probe targeting a phylogenetic signature sequence of 16s rrna of v. cholerae and v. mimicus was hybridized to rrna molecules immobilized ...200919561182
[development of taqman real-time pcr in detection of aeromonas hydrophila].to develop a taqman real-time pcr for the detection of aeromonas hydrophila.200919954074
development and degeneration of retina in rds mutant mice: altered disc shedding pattern in the heterozygotes and its relation to ocular pigmentation.in the heterozygous mutant (rds/+) mice, receptor outer segments (ros) are irregular in form and are shed as abnormally large phagosomes. in the albino rds/+ mice, peak frequency of pigment epithelial (rpe) phagosomes is higher than normal and is recorded near the end of the light period, instead of at the time of light onset as in the normal (+/+) albino mice. in pigmented mice of both genotypes, the maximum numbers of phagosomes in the rpe remain lower than in the albinos. in pigmented +/+ mic ...20092612198
an incidence of large foodborne outbreak associated with vibrio mimicus. 200918925423
more on index of refraction. 20092805311
more on index of refraction. 20092805311
anomalous origin of right pulmonary artery from the ascending aorta.anomalous origin of one pulmonary artery from the ascending aorta without associated intracardiac defects is a rare congenital malformation. about 60 anatomic and surgical descriptions have been reported in the literature. up to 1974, 22 of the 50 reported cases underwent surgical correction with a 40% mortality rate. the natural history without surgery is poor with a very high mortality during the first year of life. successful anatomic correction of anomalous origin of right pulmonary artery f ...20092777879
modulation of vibrio mimicus hemolysin through limited proteolysis by an endogenous metalloprotease.vibrio mimicus is a causative agent of human gastroenteritis and food poisoning, and this species produces an enterotoxic hemolysin (v. mimicus hemolysin) as a virulence determinant. vibrio mimicus hemolysin is secreted as an 80 kda precursor, which is later converted to the 66 kda mature toxin through removal of an n-terminal propeptide via cleavage of the arg151-ser152 bond. in this article, we investigate the role of the endogenous metalloprotease (v. mimicus protease) in the maturation of v. ...200919143841
mycotic abdominal aortic aneurysm infected by vibrio mimicus: report of a case.this report describes a case of a mycotic aneurysm of the infrarenal abdominal aorta infected by vibrio mimicus, which is the only such case ever reported in the literature. the 80-year-old male patient was first treated for gastroenteritis for 7 days. two months later, he was admitted to the hospital and the aneurysm was diagnosed by three-dimensional computed tomography. the aneurysm was excised and the restoration was done using a 16-mm dacron straight graft. the cultivation of the excised an ...200919198993
dietary supplementation of mannan oligosaccharide improves the immune responses and survival of marron, cherax tenuimanus (smith, 1912) when challenged with different stressors.three trials were conducted to determine the effects of mannan oligosaccharide (bio-mos ) on the immune responses of marron. in the first trial marron were challenged with vibrio mimicus infection, in the second with nh3 exposure and in the third, the marron were exposed to air during a simulated live transportation trial. for v. mimicus infection and live transportation trials, marron (10.44 +/- 0.20 g and 4.44 +/- 0.20 g initial weights, respectively) were fed three different diets containing ...200919539033
expression of a fusion protein containing human epidermal growth factor and the collagen-binding domain of vibrio mimicus metalloprotease.human epidermal growth factor (hegf) is a polypeptide of 53 amino acids, is an important autocrine/paracrine factor in the human body, and is used in the pharmaceutical and cosmetics industries. we constructed a fusion hegf protein with a collagen-binding domain (cbd) composed of 33 amino acids from vibrio mimicus metalloprotease (vmcbd). the cbd segment of the metalloprotease was fused at the c terminus of the hegf protein. the recombinant fusion protein was expressed in escherichia coli and pu ...200918931974
evaluating the optometric literature: the educated clinician.the purpose of this article is to present a framework for reader evaluation of optometric literature. the elements of a research article are outlined. suggestions are presented that clinicians can use to develop competence in evaluating research literature. also presented is an annotated bibliography of research references. better patient care and increased professional growth will result when clinicians evaluate and make use of the research literature.20082703662
evaluating the optometric literature: the educated clinician.the purpose of this article is to present a framework for reader evaluation of optometric literature. the elements of a research article are outlined. suggestions are presented that clinicians can use to develop competence in evaluating research literature. also presented is an annotated bibliography of research references. better patient care and increased professional growth will result when clinicians evaluate and make use of the research literature.20082703662
improvement of innate immune responses and defense activity in mitten crab (eriocheir sinensis) by oral administration of beta-glucan.the beta-glucan (bg), extracted from saccharomyces cerevisiae cell wall, was orally administrated to the mitten crab (eriocheir sinensis) at 0, 1, 5 and 10 g bg/kg diet for 28 days, followed by a challenge with vibrio mimicus by intramuscular injection. growth, phenoloxidase, superoxide dismutase, acid phosphatase and alkaline phosphatase activity were monitored after 14 and 28 days. the results showed an immunomodulatory effect and protection against v. mimicus by dietary supplementation of bg. ...200818568402
a methoxylated fatty acid isolated from the brown seaweed ishige okamurae inhibits bacterial phospholipase a2.a methoxylated fatty acid that inhibits phospholipase a(2) (pla(2); ec 3.1.1.4) was purified from the brown seaweed ishige okamurae. approximately 8.1 mg of the inhibitory compound, 7-methoxy-9-methylhexadeca-4,8-dienoic acid, was isolated from 1 kg of i. okamurae powder. recombinant pla(2) derived from the pathogenic bacterium vibrio mimicus was used as the target enzyme. the methoxylated fatty acid compound competitively inhibited pla(2) with a ki value of 3.9 microg/ml. the concentrations req ...200818570268
distortion product emissions and sensorineural hearing loss.distortion product emissions (dpes) are intimately linked to normal outer hair cell function. outer hair cell function is itself intimately linked with normal auditory thresholds. we sought to correlate frequency-specific dpe measurements with auditory thresholds, in seven frequency regions from 700 hz to 6 khz, in each of 21 ears. eleven of these ears had thresholds below 25 db spl at every test frequency, whereas 10 ears demonstrated some degree of purely sensorineural hearing loss. an analysi ...20082593222
identification of vibrio cholerae and vibrio mimicus by multilocus sequence analysis (mlsa).vibrio cholerae and vibrio mimicus have similar phenotypes and genomes making rapid differentiation of these two species difficult. the first standard multilocus sequence analysis (mlsa) scheme for the identification of these species is described. a collection of 45 representative isolates from different geographical regions and hosts was examined using segments of the housekeeping genes pyrh, reca and rpoa. overall, the closest phylogenetic neighbours of these species were vibrio furnissii and ...200818319466
vibrio porteresiae sp. nov., a diazotrophic bacterium isolated from a mangrove-associated wild rice (porteresia coarctata tateoka).two facultatively anaerobic, nitrogen-fixing bacteria (strains mssrf30(t) and mssrf31) were isolated from a mangrove-associated wild rice (porteresia coarctata tateoka). these strains were determined to be nitrogen-fixers using the acetylene reduction assay and by pcr detection of a nifh gene amplicon. phylogenetic analyses based on 16s rrna gene sequences indicated that the novel strains were most closely related to vibrio fluvialis lmg 7894(t) (96.8 % gene sequence similarity), vibrio furnissi ...200818599703
a randomized trial of anticoagulation with warfarin and of alternating chemotherapy in extensive small-cell lung cancer by the cancer and leukemia group b.the cancer and leukemia group b (calgb) conducted a prospective randomized trial to evaluate the role of warfarin and alternating chemotherapy in extensive small-cell lung cancer (sccl). after stratification for sex and performance status, patients were randomly assigned to receive chemotherapy with methotrexate, doxorubicin (adriamycin; adria laboratories, columbus, oh), cyclophosphamide, and lomustine (ccnu) (macc), or macc plus warfarin (macc + w), or mitomycin, etoposide, cisplatin, and hexa ...20082547030
[atypical and combined forms of infectious-allergic polyneuropathies]. 20071865642
haemolysin produced by vibrio mimicus activates two cl- secretory pathways in cultured intestinal-like caco-2 cells.haemolysin (vmh) is a virulent factor produced by vibrio mimicus, a human pathogen that causes diarrhoea. as intestinal epithelial cells are the primary targets of haemolysin, we investigated its effects on ion transport in human colonic epithelial caco-2 cells. vmh increased the cellular short circuit current (isc), used to estimated ion fluxes, and 125i efflux of the cells. the vmh-induced increases in isc and 125i efflux were suppressed by depleting ca2+ from the medium or by pretreating the ...200717026482
catecholamine-induced stimulation of growth in vibrio species.to evaluate the effect of norepinephrine (ne) and related compounds on the growth of bacteria, we have examined the effect of the neuroendocrine hormone ne and related compounds on the growth of vibrio parahaemolyticus and other human-pathogenic vibrio species (vibrio cholerae, vibrio vulnificus, and vibrio mimicus).200717576228
rapid and specific identification of 5 human pathogenic vibrio species by multiplex polymerase chain reaction targeted to dnaj gene.a multiplex polymerase chain reaction (pcr) method, specifically designed for application in routine diagnostic laboratories, was developed for identifying 5 human pathogen vibrio species: vibrio cholerae, vibrio parahaemolyticus, vibrio vulnificus, vibrio mimicus, and vibrio alginolyticus. this assay directed toward the dnaj gene was tested on a total of 355 strains representing 13 vibrio species and 17 non-vibrio species. specific pcr fragments were produced in isolates belonging to the 5 targ ...200717614235
identification of vibrio isolates by a multiplex pcr assay and rpob sequence determination.vibrio, a diverse genus of aquatic bacteria, currently includes 72 species, 12 of which occur in human clinical samples. of these 12, three species--vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus-account for the majority of vibrio infections in humans. rapid and accurate identification of vibrio species has been problematic because phenotypic characteristics are variable within species and biochemical identification requires 2 or more days to complete. to facilitate the identifi ...200717093013
intracerebral hemorrhage, cerebral infarction, and subdural hematoma after acute myocardial infarction and thrombolytic therapy in the thrombolysis in myocardial infarction study. thrombolysis in myocardial infarction, phase ii, pilot and clinical trial.in the thrombolysis in myocardial infarction, phase ii pilot and clinical trial, 908 patients [326 (35.9%) in the pilot study and 582 (64.0%) in the randomized study] were treated with 150 mg recombinant tissue-type plasminogen (rt-pa) activator in combination with heparin and aspirin, and 3,016 patients [64 (2.1%) in the pilot study and 2,952 (97.9%) in the randomized study] were treated with 100 mg rt-pa in combination with heparin and aspirin. adverse neurological events occurred in 23 patien ...20071899364
comparison between end-systolic pressure-volume and end-systolic wall stress in determining left ventricular contractility with increased afterload.this study compared end-systolic pressure-volume relationship (p/v) versus wall stress index (ws) as a tool for the evaluation of ventricular contractile state during upright isometric exercise. both indices of contractility exhibit significant (p < 0.001) increase during exercise compared to resting values. the correlation coefficients for the two indices of contractility were r = -0.45 for exercise and r = -0.70 for rest. both were significant at the p < 0.01 level. the correlation of the diff ...20061477857
effect of antihypertensive drugs on insulin, glucose, and lipid metabolism.the close relationship between diabetes and hypertension has been recognized for decades. new information indicates that resistance to insulin action on glucose uptake in peripheral tissues is a common underlying mechanism in hypertension and diabetes. in prospective trials, the effects of antihypertensive agents on insulin sensitivity and lipoprotein metabolism have been evaluated. both beta-blockers and thiazide diuretics worsen insulin resistance and deteriorate lipoprotein metabolism. angiot ...20061828417
effect of antihypertensive drugs on insulin, glucose, and lipid metabolism.the close relationship between diabetes and hypertension has been recognized for decades. new information indicates that resistance to insulin action on glucose uptake in peripheral tissues is a common underlying mechanism in hypertension and diabetes. in prospective trials, the effects of antihypertensive agents on insulin sensitivity and lipoprotein metabolism have been evaluated. both beta-blockers and thiazide diuretics worsen insulin resistance and deteriorate lipoprotein metabolism. angiot ...20061828417
the value of an expert system in performing clinical drug trials.an expert system has been designed to assist the cardiologist in determining whether patients can be included in clinical trials. this system contains knowledge on inclusion and exclusion criteria for six drug trials, and has been validated in 100 randomly selected patients. in 97 cases, the expert system and the cardiologist made an identical classification; in the remaining three cases, the patient was incorrectly classified by the physician. the system will also optimize the order in which qu ...20061764928
proteolytic activation of vibrio mimicus (vm) major outer membrane protein haemagglutinin (ha) with vm-ha/protease: implication for understanding bacterial adherence.vibrio mimicus (vm) haemagglutinins (has), such as an extracellular ha/protease (vm-ha/protease) and a major outer membrane protein-ha (vm-ompha), have been recognized as the putative adherence factors for the bacterium. however, the mechanism by which has coordinate the adherence function of the bacterium remains as yet unknown. we report herein the positive interaction between vm-ha/protease and vm-ompha resulting in significant enhancement of the haemagglutinating ability. in this interaction ...200617116978
intravenous nifedipine for prevention of myocardial ischaemia after coronary revascularization.we sought to determine the pharmacokinetic and pharmacodynamic behaviour of a continuous infusion of nifedipine given for prevention of myocardial ischaemia following coronary artery bypass graft (cabg) surgery. patients scheduled for elective cabg, who had good left ventricular function, were included. only normotensive patients who did not require treatment with vasoactive drugs and were bleeding less than 100 ml.hr-1 following surgery were included. the patients were randomly distributed into ...20061464126
haemorheology and exercise: intrinsic flow properties of blood in marathon running.haemorheological and haematological parameters were studied in venous blood samples taken from 8 endurance-trained athletes before and after a marathon run. viscometry was performed in a 20 microns glass capillary and in a couette viscometer. apparent blood viscosity was lower in the capillary than in the rotational viscometer, but significant differences between pre- and post-run samples were not observed in either system. this is presumably due to the absence of changes of haematocrit (pre-run ...20061459744
pcr-based identification of vibrio cholerae and the closely related species vibrio mimicus using the large chromosomal ori sequence of vibrio cholerae.the bacterial chromosomal replication origin (ori) sequences are a highly conserved essential genetic element. in this study, the large chromosomal replication origin sequence of vibrio cholerae (oricivc) has been targeted for identification of the organism, including the biotypes of serogroup o1. the oricivc sequence-based pcr assay specifically amplified an 890 bp fragment from all the v. cholerae strains examined. a point mutation in the oricivc sequence of the classical biotype of o1 serogro ...200616553836
presence of luxs/ai-2 based quorum-sensing system in vibrio mimicus : luxo controls protease activity.presence of the quorum-sensing regulation system in vibrio mimicus was investigated. the culture supernatants of v. mimicus strains were found to possess ai-2 autoinducer like activity, and the strains were found to harbor the genes which are homologous to luxs, luxo, and luxr of v. harveyi. these genes of v. harveyi have been shown to be important components of v. harveyi-like quorum-sensing system. the luxo gene homologue known to encode luxo, the central component of the regulation system, wa ...200616714849
development of monoclonal antibodies for simple identification of vibrio alginolyticus.the present study was aimed to produce monoclonal antibodies (mabs) for simple and specific identification of vibrio alginolyticus infection in shrimp.200616965376
isolation of vibrio mimicus from a case of acute diarrhoea--a case report.over a four months period (june to september, 2003) 111 stool samples from cases of acute diarrhoea were screened for vibronaceae in department of microbiology, smt. n. h. l. municipal medical college, ahmedabad. all the isolates were identified by standard laboratory technique. a total of 36 species belonging to vibrionaceae were isolated. phage typing of all these vibronaceae were done at national institute of cholera and enteric disease, kolkata. out of 36 isolates, 35 were vibrio cholereae a ...200617001922
health checks on patients 75 years and over in nottinghamshire after the new gp contract.to investigate annual health checks for patients of 75 years and over required by the 1990 contract for general practitioners.20061393076
organization of the ctx prophage in environmental isolates of vibrio mimicus.the organization of the ctx prophage in environmental strains of vibrio mimicus was investigated. sixteen hundred non-sucrose fermenting vibrios were examined for ctx gene by hybridization. out of 1,600 isolates, 6 v. mimicus isolates contained ctxa gene. the organization of ctx prophage was determined by rflp using ctxa probe. the ctx prophage integrated at a single site in v. mimicus genome which was present as a single copy flanked by at least a single rs element. ribotype pattern revealed th ...200516113506
a simple centrifuge column for desalting protein solutions. 2005543535
use of oligonucleotide array for identification of six foodborne pathogens and pseudomonas aeruginosa grown on selective media.identification of presumptive foodborne pathogens grown on selective media may take one to several days and requires a different battery of biochemical tests for each microorganism. a molecular identification method was developed in which universal primers were used to amplify the 16s to 23s rdna intergenic spacer of target microorganisms, and pcr products were hybridized to a panel of species-specific oligonucleotides that were immobilized on a nylon membrane. the seven target microorganisms we ...200516300063
a hemolysin of vibrio mimicus (vmh) stimulates cells to produce atp and cyclic amp which appear to be secretory mediators.the hemolysin of vibrio mimicus(vmh) is a pore-forming toxin with both enterotoxic and hemolytic activity. the hemolysis by vmh is induced by creation of pores in the membrane of erythrocyte; however, the mechanism for the enterotoxic action of vmh has remained unclear. in order to clarify the mechanism, we incubated t84 cells (a human colon carcinoma cell line) with vmh and found that the levels of atp and cyclic amp of culture medium increased after exposure of the cells to vmh. subsequently, ...200515665456
biochemical characterization of a cambialistic superoxide dismutase isozyme from diatom thallassiosira weissflogii: cloning, expression, and enzyme stability.a cdna clone of 1081 bp encoding a second putative superoxide dismutase (sod) from diatom thallassiosira weissflogii was cloned by the polymerase chain reaction technique. the cdna encodes a protein of 286 amino acid residues. alignment of the truncated sod sequence containing 217 amino acid residues with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed greater homology to mn-sod. the residues required to coordin ...200516076113
[massive lower gastrointestinal hemorrhage of diverticular origin. diagnostic and therapeutic problems]. 20051082809
[pathogenic factors of vibrios with special emphasis on vibrio vulnificus].bacteria of the genus vibrio are normal habitants of the aquatic environment and play roles for biocontrole of aquatic ecosystem, but some species are believed to be human pathogens. these species can be classified into two groups according to the types of diseases they cause: the gastrointestinal infections and the extraintestinal infections. the pathogenic species produce various pathogenic factors including enterotoxin, hemolysin, cytotoxin, protease, siderophore, adhesive factor, and hemaggl ...200515997210
the faxwxxt motif in the carboxyl terminus of vibrio mimicus metalloprotease is involved in binding to collagen.we have shown previously that the c-terminal region of the extracellular metalloprotease of vibrio mimicus (vmc) is essential for collagenase activity. here, we demonstrate that deletion of 100 amino acids, but not 67 amino acids, from the c-terminus of the intact vmc protein (vmc61) abolished the collagenase activity. the intervening 33-amino acid region contains a repeated faxwxxt motif that is essential for insoluble type i collagen binding; the isolated 33-amino acid peptide bound to insolub ...200515848196
male transsexualism in england: sixteen cases with surgical intervention.the syndrome of transsexualism is now regarded as a dinstinct entity separate from transvestism and homosexuality (benjamin, 1966; green and money, 1969). it refers to those patients who since childhood have been convinced of their wrong sex assignment and who wish to attain the sexual characteristics of the opposite sex and to live as a member of that sex. the present report is an initial one referring to the first group of transsexual patients studied at a postgraduate teaching hospital by a m ...20051169047
selectivity and specificity of a chromogenic medium for detecting vibrio parahaemolyticust.the thiosulfate-citrate-bile salts-sucrose agar (tcbs) used in the most-probable-number method for detecting vibrio parahaemolyticus cannot differentiate growth of v. parahaemolyticus from vibrio vulnificus or vibrio mimicus. this study examined the selectivity and specificity of bio-chrome vibrio medium (bcvm), a chromogenic medium that detects v. parahaemolyticus on the basis of the formation of distinct purple colonies on the medium. a panel consisting of 221 strains of bacteria, including 17 ...200516013386
molecular evolution of vibrio pathogenicity island-2 (vpi-2): mosaic structure among vibrio cholerae and vibrio mimicus natural isolates.vibrio cholerae is a gram-negative rod that inhabits the aquatic environment and is the aetiological agent of cholera, a disease that is endemic in much of southern asia. the 57.3 kb vibrio pathogenicity island-2 (vpi-2) is confined predominantly to toxigenic v. cholerae o1 and o139 serogroup isolates and encodes 52 orfs (vc1758 to vc1809), which include homologues of an integrase (vc1758), a restriction modification system, a sialic acid metabolism gene cluster (vc1773-vc1783), a neuraminidase ...200515632448
molecular typing of epidemic and nonepidemic vibrio cholerae isolates and differentiation of v. cholerae and v. mimicus isolates by pcr-single-strand conformation polymorphism analysis.to examine the utility of polymerase chain reaction (pcr)-single-strand conformation polymorphism (sscp) analysis to differentiate epidemic and nonepidemic vibrio cholerae isolates as well as to differentiate v. cholerae and vibrio mimicus isolates.200515715856
characterization of fe/mn-superoxide dismutase from diatom thallassiosira weissflogii: cloning, expression, and property.a cdna clone of 1114 bp encoding a putative mn-superoxide dismutase (mn-sod) from diatom thallassiosira weissflogii was cloned by the pcr technique. nucleotide sequence analysis of this cdna clone revealed that it was translated into 201 amino acid residues. when the sequence was compared with mn-sods from vibrio mimicus and escherichia coli, as well as two fe-sods from e. coli and photobacterium leiognathi, this sod showed higher homology to mn-sod. the amino acid residues required to coordinat ...200515740026
electrocardiographic changes under moderate hypoxia. 2005546723
proceedings: the transient effect of atp on k movement in hela cells. 20051151801
[choledochoplasty in the treatment of cholelithiasis]. 2004516291
management of bone in digital replantation: free vascularized and composite bone grafts.microvascular surgical techniques have been applied to the problem of digital amputation, persistent nonunion and complicated extremity injuries with segmental bone and soft tissue loss. in replantations, adequate bone shortening and fixation with intramedullary pins or rods is mandatory. proper patient selection, preoperative management of the amputated parts, application of microsurgical disciplines, employment of an organized surgical sequence, and rigid postoperative monitoring has resulted ...2004357062
use of reca as an alternative phylogenetic marker in the family vibrionaceae.this study analysed the usefulness of reca gene sequences as an alternative phylogenetic and/or identification marker for vibrios. the reca sequences suggest that the genus vibrio is polyphyletic. the high heterogeneity observed within vibrios was congruent with former polyphasic taxonomic studies on this group. photobacterium species clustered together and apparently nested within vibrios, while grimontia hollisae was apart from other vibrios. within the vibrios, vibrio cholerae and vibrio mimi ...200415143042
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