Publications

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inactivation of vibrio parahaemolyticus in shucked raw oyster ( grassostrea gigas) and clam ( venerupis phillippinarum) by using a combination of naclo and gamma irradiation.this study investigated the synergistic effects of sodium hypochlorite (naclo) and gamma irradiation combination against vibrio parahaemolyticus in shucked oysters and clams. v. parahaemolyticus decreased to 1.1-5.6 log10 cfu/g in oysters and 1.1-5.7 log10cfu/g in clams by naclo (20-80 ppm) + γ irradiation (0.1-2.0 kgy) combinations. v. parahaemolyticus was not detected by 60 or 80 ppm naclo + 2.0 kgy. synergistic reduction of >1 log was observed by 60 ppm naclo + 0.3-2 kgy and 80 ppm naclo + 0. ...201728840748
microbiome dynamics in a shrimp grow-out pond with possible outbreak of acute hepatopancreatic necrosis disease.acute hepatopancreatic necrosis disease (ahpnd) (formerly, early mortality syndrome) is a high-mortality-rate shrimp disease prevalent in shrimp farming areas. although ahpnd is known to be caused by pathogenic vibrio parahaemolyticus hosting the plasmid-related pirab(vp) toxin gene, the effects of disturbances in microbiome have not yet been studied. we took 62 samples from a grow-out pond during an ahpnd developing period from days 23 to 37 after stocking white postlarvae shrimp and sequenced ...201728839269
in vitro isothermal nucleic acid amplification assisted surface-enhanced raman spectroscopic (sers) for ultrasensitive detection of vibrio parahaemolyticus.detection of pathogenic microorganisms is of great importance for public health and food safety. traditional protocols can hardly meet the continuously increased demanding in sensitivity and specificity of pathogen detections. in this study, we adopted vibrio parahaemolyticus (v. parahaemolyticus, vp) as the model analyte, and developed an antibody-vp-aptamer hetero-sandwich based surface-enhanced raman scattering (sers) method in conjunction with in vitro isothermal amplification for sensitive ...201728832123
proteomic analysis of vibrio parahaemolyticus under cold stress.vibrio parahaemolyticus is a kind of food-borne pathogenic bacterium, which can seriously infect food, especially seafood causing gastroenteritis and other disease. we studied the global proteome responses of v. parahaemolyticus under cold stress by nano-liquid chromatography-tandem mass spectrometry to improve the present understanding of v. parahaemolyticus proteomics events under cold stress. a total of 1151 proteins were identified and 101 proteins were differentially expressed, of which 69 ...201728831596
characterization, expression, and evolutionary analysis of new tlr3 and tlr5m genes cloned from the spiny eel mastacembelus armatus.toll-like receptors (tlrs) play an important role in innate and adaptive immunity. here, we identify two new tlrs from the spiny eel mastacembelus armatus (tlr3 and membrane tlr5m). both matlr3 and matlr5m were expressed in all tested tissues; expression was highest in liver and spleen, respectively. after infection with vibrio parahaemolyticus, expression of both tlrs fluctuated and differed significantly from controls at several time points. the predicted three-dimensional model of the matlr3 ...201728821419
christmas-tree derived amplification immuno-strategy for sensitive visual detection of vibrio parahaemolyticus based on gold label silver stain technology.a developed christmas-tree derived immunosensor based on a gold label silver stain (glss) technique was fabricated for a highly sensitive analysis of vibrio parahaemolyticu (vp). in this strategy, captured vp antibody (cab) was immobilized on a solid substrate; then, the vps were sequentially tagged with a signal probe by incubating the assay with a detection vp antibody (dab) conjugated gold nanoparticles (aunps)-labeled graphite-like carbon nitride (g-c3n4). finally, the attached signal probe ...201728794324
insight into the origin and evolution of the vibrio parahaemolyticus pandemic strain.a strain of vibrio parahaemolyticus that emerged in 1995 caused the first known pandemic involving this species. this strain comprises clonal autochthonous ocean-dwelling bacteria whose evolution has occurred in the ocean environment. the low sequence diversity in this population enabled the discovery of information on its origin and evolution that has been hidden in bacterial clones that have evolved over a long period. multilocus sequencing and microarray analysis, together with phylogenetic a ...201728790994
distribution and antimicrobial susceptibility of vibrio species associated with zooplankton in coastal area of korea.vibrio parahaemolyticus and v. vulnificus are the most common pathogens causing seafood-borne illnesses in korea. this study determines the abundance and antimicrobial resistance of pathogenic vibrio species in seawater and zooplankton samples from the geoje island coast in korea, which is an important area for coastal fisheries, the fishing industry, and tourism. the two vibrio species were detected more in mesozooplankton samples than in seawater samples. v. parahaemolyticus isolates showed gr ...201728781188
function of two ficolin-like proteins in innate immune defense of the oriental river prawn, macrobrachium nipponense.ficolins have crucial functions in recognizing and eliminating pathogens in innate immunity. in this study, we identified two ficolin-like genes from the oriental river prawn, macrobrachium nipponense. these genes were designated as mnfico1 and mnfico2. mnfico1 cdna has 1600 bp, whereas mnfico2 has 1486 bp. in addition to a coiled-coil region or a low complexity region, the two ficolins both contained a signal peptide and a fibrinogen-related domain. qrt-pcr results showed that the highest expre ...201728764985
nmr-based metabolomics reveals the metabolite profiles of vibrio parahaemolyticus under ferric iron stimulation.vibrio parahaemolyticus is a halophilic bacterium endemic to coastal areas, and its pathogenicity has caused widespread seafood poisoning. in our previous research, the protein expression of v. parahaemolyticus in fe(3+) medium was determined using isobaric tags for relative and absolute quantitation (itraq). here, nuclear magnetic resonance (nmr) was used to detect changes in the v. parahaemolyticus metabolome. nmr spectra were obtained using methanol-water extracts of intracellular metabolites ...201728752295
identification of an mitf gene and its polymorphisms associated with the vibrio resistance trait in the clam meretrix petechialis.microphthalmia-associated transcription factor (mitf) regulates the transcription of its target genes by binding to their promoters. in this study, an mitf gene, mpmitf was identified in the clam meretrix petechialis. the full-length cdna of mpmitf is 3564 bp with an orf of 1365 bp. the deduced amino acid sequence consists of a conserved functional structure of bhlh-lz, which could bind with e-box. the mrna and protein expression levels of mpmitf were significantly up-regulated 6 h post-vibrio i ...201728734968
characterizing the adherence profiles of virulent vibrio parahaemolyticus isolates.the human pathogen vibrio parahaemolyticus is a leading cause of seafood-borne illness in the usa, and infections with v. parahaemolyticus typically result from eating raw or undercooked oysters. v. parahaemolyticus has been shown to be highly resistant to oyster depuration, suggesting that the bacterium possesses specific mechanisms or factors for colonizing oysters and persisting during depuration. in this study, we characterized eight different v. parahaemolyticus strains for differences in r ...201728717834
antibacterial activity of food-grade chitosan against vibrio parahaemolyticus biofilms.biofilm is a community composed of microbes and the extracellular polymeric substances. this special architecture poses a significant public health risk as it increases the fitness of bacteria in harsh conditions and renders bacterial resistance to antimicrobial agents and cleaning. in this study, we investigated the inhibition and eradication effects of chitosan on the biofilm of vibrio parahaemolyticus, an important food-borne pathogen. the crystal violet staining, [2, 3-bis (2-methoxy-4-nitro ...201728710011
molecular characterization and expression analyses of toll like receptor-5 induced by vibrio parahaemolyticus antigens in pacific red snapper.toll-like receptor 5 (tlr5) is a member of tlrs family responsible for the bacterial flagellin recognition in vertebrates. herein, the tlr5m gene structure of pacific red snapper (lutjanus peru) was characterized. the full-length cdna of lptlr5m comprises an open reading frame (orf) of 2715 bp, encoding a polypeptide of 904 amino acids including 9 lrrs (residues 119-562) and one lrr-ct domain (residues 593-646) at the extracellular region, and a tir domain (residues 710-904) in the cytoplasmic r ...201728698126
parallel evolution of two clades of a major atlantic endemic vibrio parahaemolyticus pathogen lineage by independent acquisition of related pathogenicity islands.shellfish-transmitted vibrio parahaemolyticus infections have recently increased from locations with historically low disease incidence, such as the northeast united states (us). this change coincided with a bacterial population shift towards human pathogenic variants occurring in part through the introduction of several pacific native lineages (st36, st43 and st636) to near-shore areas off the atlantic coast of the northeast us. concomitantly, st631 emerged as a major endemic pathogen. phylogen ...201728687650
characterization of clinical vibrio parahaemolyticus strains in zhoushan, china, from 2013 to 2014.vibrio parahaemolyticus is recognized as major cause of foodborne illness of global public health concern. this study collected 107 strains of v. parahaemolyticus during active surveillance of diarrheal diseases in hospitals in zhoushan during 2013 to 2014 and investigated their serotypes, virulence genes (tdh, trh, and orf8), antimicrobial resistance, and genotypes. the dominant serotypes of the 107 clinical strains were o3:k6, o4:k8, and o4:kut with 87.9% and 3.7% of the strains carrying the v ...201728678810
prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from short mackerels (rastrelliger brachysoma) in malaysia.numerous prevalence studies and outbreaks of vibrio parahaemolyticus infection have been extensively reported in shellfish and crustaceans. information on the quantitative detection of v. parahaemolyticus in finfish species is limited. in this study, short mackerels (rastrelliger brachysoma) obtained from different retail marketplaces were monitored with the presence of total and pathogenic strains of v. parahaemolyticus. out of 130 short mackerel samples, 116 (89.2%) were detected with the pres ...201728659901
involvement of a tachylectin-like gene and its protein in pathogenesis of acute hepatopancreatic necrosis disease (ahpnd) in the shrimp, penaeus monodon.a shrimp disease, the so-called acute hepatopancreatic necrosis disease (ahpnd) is caused by a specific strain of vibrio parahaemolyticus (vp) and it has resulted in significant losses to the global shrimp farming industry. in our previous study, three of tachylectin-like genes were cloned and characterized from the intestine of penaeus monodon, designated as penlectin5-1 (pl5-1), penlectin5-2 (pl5-2) and penlectin5-3 (pl5-3). these three genes all contain fibrinogen-related domain (fred). the e ...201728655576
transcriptome analysis of the hepatopancreas in exopalaemon carinicauda infected with an ahpnd-causing strain of vibrio parahaemolyticus.acute hepatopancreatic necrosis disease (ahpnd) caused by vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in multiple shrimp species throughout asia. in order to understand the immunological response of shrimp to infection by ahpnd-causing strain of v. parahaemolyticus (vpahpnd), the transcriptomic profiles of the hepatopancreas from severe ahpnd-infected (bs_g), ahpnd-survived (kb_g) and non-infected (pbs_g) exopalaemon carinicauda groups were obtained us ...201728648882
a comprehensive epidemiological research for clinical vibrio parahaemolyticus in shanghai.vibrio parahaemolyticus is one of the most important pathogen for seafood-borne gastroenteritis in shanghai and the rest of the world. a total of 42 v. parahaemolyticus strains were isolated from 1900 fecal specimens collected from patients in shanghai hospital presenting from january 2014 to december 2015. all isolates were evaluated for potential virulence factors [tdh, trh, and type three secretion system (t3ss) genes], typed using multilocus sequence typing (mlst) and screened for antimicrob ...201728642752
t3ss effector vopl inhibits the host ros response, promoting the intracellular survival of vibrio parahaemolyticus.the production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (nadph) oxidase complex is an important mechanism for control of invading pathogens. herein, we show that the gastrointestinal pathogen vibrio parahaemolyticus counteracts reactive oxygen species (ros) production using the type iii secretion system 2 (t3ss2) effector vopl. in the absence of vopl, intracellular v. parahaemolyticus undergoes ros-dependent filamentation, with concurrent limited growth ...201728640881
comparative transcriptome analysis of sinonovacula constricta in gills and hepatopancreas in response to vibrio parahaemolyticus infection.the razor clam sinonovacula constricta is an important economic species in china. however, bacterial pathogenic diseases limits s. constricta farming industry for large-scale production. in this study, de novo transcriptome sequencing was performed on s. constricta gills and hepatopancreas under vibrio parahaemolyticus challenge for 12 h and 48 h, respectively. transcripts assembly constructed 18,330 sequences, each of which was 500 bp long and functionally annotated, and 1781 and 490 transcript ...201728630016
survival of litopenaeus vannamei shrimp fed on diets supplemented with dunaliella sp. is improved after challenges by vibrio parahaemolyticus.survival of litopenaeus vannamei was evaluated during a vibrio parahaemolyticus infection. this bacteria has been causing significant economic losses in the shrimp industry due to the appearance of early mortality syndrome (ems), also known as acute hepatopancreatic necrosis disease (ahpnd). control of bacteria in ponds is difficult to achieve with antibiotics due to environmental infections and antibiotic resistance. new methods have been proposed to control and prevent the impact of bacterial ...201728625840
pathogenicity profile of vibrio parahaemolyticus in farmed pacific white shrimp, penaeus vannamei.a pathobiological study was conducted using vibrio parahaemolyticus (vp) strain isolated from vibriosis affected shrimp (penaeus vannamei) farms in kancheepuram and thiruvallur districts of tamil nadu during august 2014 to february 2015. the isolate was identified based on the morphological, physiological, biochemical and molecular characters. ld50 value with intramuscular injection was determined as 2.6 × 10(4) cfu/shrimp and sequential pathology was studied giving 6.1 × 10(3) cfu/shrimp (ld25) ...201728606862
genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (vpacspa and vpacspd) in vibrio parahaemolyticus chn25 during low-temperature survival.vibrio parahaemolyticus causes serious seafood-borne gastroenteritis and death in humans. raw seafood is often subjected to post-harvest processing and low-temperature storage. to date, very little information is available regarding the biological functions of cold shock proteins (csps) in the low-temperature survival of the bacterium. in this study, we determined the complete genome sequence of v. parahaemolyticus chn25 (serotype: o5:kut). the two main csp-encoding genes (vpacspa and vpacspd) w ...201728583064
a natural vibrio parahaemolyticus pir(vp)a(-)b(+) mutant kills shrimp but produces no pir(vp) toxins or ahpnd lesions.acute hepatopancreatic necrosis disease (ahpnd) of shrimp is caused by vibrio parahaemolyticus (vp) isolates that harbor a pva plasmid encoding toxins pir(vp)a and pir(vp)b (vpahpnd). these are released from vpahpnd that colonize the shrimp stomach and produce pathognomonic ahpnd lesions (massive sloughing of hepatopancreatic tubule epithelial cells). pcr results indicated that vp isolate xn87 lacked pir(vp)a but carried pir(vp)b unexpectedly, western blot analysis of proteins from culture broth ...201728576761
spreading of pandemic vibrio parahaemolyticus o3:k6 and its serovariants: a re-analysis of strains isolated from multiple studies.in china, v. parahaemolyticus has been a leading cause of foodborne outbreaks and bacterial infectious diarrhea since the 1990s, and most infections have been associated with the pandemic v. parahaemolyticus o3:k6 and its serovariants. however, a comprehensive overview of the sero-prevalence and genetic diversity of the pandemic v. parahaemolyticus clone in china is lacking. to compensate for this deficiency, pandemic isolates in both clinical and environmental chinese samples collected from mul ...201728573108
induction of cellular differentiation and single cell imaging of vibrio parahaemolyticus swimmer and swarmer cells.the ability to study the intracellular localization of proteins is essential for the understanding of many cellular processes. in turn, this requires the ability to obtain single cells for fluorescence microscopy, which can be particularly challenging when imaging cells that exist within bacterial communities. for example, the human pathogen vibrio parahaemolyticus exists as short rod-shaped swimmer cells in liquid conditions that upon surface contact differentiate into a subpopulation of highly ...201728570527
different abundance and correlational patterns exist between total and presumed pathogenic vibrio vulnificus and v. parahaemolyticus in shellfish and waters along the north carolina coast.monitoring of vibrio vulnificus and v. parahaemolyticus abundance is pertinent due to the ability of these species to cause disease in humans through aquatic vectors. previously, we performed a multiyear investigation tracking vibrio spp. levels in five sites along the southeastern north carolina coast. from february 2013 to october 2015, total v. vulnificus and v. parahaemolyticus abundance was measured in water, oysters and clams. in the current study, pathogenic subpopulations were identified ...201728531284
gene expression of vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment.vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °c, a condition seldom found in the ocean. however, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. in this context, revealing how gene expression enables v. parahaemolyticus to adapt to this particular condition -common to almost all v. parahaemolyticus isolates- will improve our understan ...201728525966
molecular typing of vibrio parahaemolyticus strains isolated from mollusks in the north adriatic sea.vibrio parahaemolyticus is an emerging foodborne pathogen in the mediterranean, usually associated with shellfish consumption. the increase in the number of outbreaks in europe is primarily associated with the global warming of the ocean that has a great impact on the spread and genetic selection of waterborne pathogens. the primary role of italy in europe's mollusk production, together with the fact that cases of infections with v. parahaemolyticus are not always notified to the european commun ...201728514180
science signaling podcast for 16 may 2017: vibrio rewires host cells.this podcast features a conversation with kim orth and nicole de nisco, authors of a research resource that appears in the 16 may 2017 issue of science signaling, about how the marine bacterium vibrio parahaemolyticus rewires host cell signaling networks. v. parahaemolyticus thrives in warm brackish waters and infects both shellfish and finfish. this bacterium causes gastroenteritis when humans consume contaminated seafood that is raw or undercooked. v. parahaemolyticus delivers virulence factor ...201728512146
the cytotoxic type 3 secretion system 1 of vibrio rewires host gene expression to subvert cell death and activate cell survival pathways.bacterial effectors potently manipulate host signaling pathways. the marine bacterium vibrio parahaemolyticus (v. para) delivers effectors into host cells through two type 3 secretion systems (t3sss). t3ss1 is vital for v. para survival in the environment, whereas t3ss2 causes acute gastroenteritis in human hosts. although the natural host is undefined, t3ss1 effectors attack highly conserved cellular processes and pathways to orchestrate nonapoptotic cell death. to understand how the concerted ...201728512145
new sequence types of vibrio parahaemolyticus isolated from a malaysian aquaculture pond, as revealed by whole-genome sequencing.the acquisition of photorhabdus insect-related (pir) toxin-like genes in vibrio parahaemolyticus has been linked to hepatopancreatic necrosis disease in shrimp. we report the whole-genome sequences of genetically virulent and avirulent v. parahaemolyticus isolated from a malaysian aquaculture pond and show that they represent previously unreported sequence types of v. parahaemolyticus.201728495773
isolation of bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by vibrio parahaemolyticus.acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by vibrio parahaemolyticus (ahpnd vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to photorhabdus insect-related toxins. we obtained 9 isolates of bdellovibrio and like organisms (balos) from water and sediment samples in thailand. using 16s rrna sequencing, all of the organisms were identified as bacteriovorax spp. and were able to attack all tested ahpnd vp isolates. in addition, ...201728492178
pathogenic vibrio species isolated from estuarine environments (ceará, brazil) - antimicrobial resistance and virulence potential profiles.detection of virulent strains associated with aquatic environment is a current concern for the management and control of human and animal health. thus, vibrio diversity was investigated in four estuaries from state of ceará (pacoti, choró, pirangi and jaguaribe) followed by antimicrobial susceptibility to different antimicrobials used in aquaculture and detection of main virulence factors to human health. isolation and identification were performed on tcbs agar (selective medium) and dichotomous ...201728489191
bile sensing: the activation of vibrio parahaemolyticus virulence.bacteria must develop resistance to various inhospitable conditions in order to survive in the human gastrointestinal tract. bile, which is secreted by the liver, and plays an important role in food digestion also has antimicrobial properties and is able to disrupt cellular homeostasis. paradoxically, although bile is one of the guts defenses, many studies have reported that bacteria such as vibrio parahaemolyticus can sense bile and use its presence as an environmental cue to upregulate virulen ...201728484445
effects of ambient exposure, refrigeration, and icing on vibrio vulnificus and vibrio parahaemolyticus abundances in oysters.vibrio vulnificus (vv) and v. parahaemolyticus (vp) illnesses are typically acquired through the consumption of raw molluscan shellfish, particularly oysters. as vibrio spp. are naturally-occurring bacteria, one means of mitigation of illness is achieved by limiting post-harvest growth. in this study, effects of ambient air storage, refrigeration, and icing of oysters on vibrio spp. abundances were examined at two sites in alabama (al) [dog river (dr) and cedar point (cp)] and one site in delawa ...201728482282
a sulfated galactans supplemented diet enhances the expression of immune genes and protects against vibrio parahaemolyticus infection in shrimp.a sulfated galactans (sg) supplemented diet was evaluated for the potential to stimulate immune activity in shrimp penaeus vannamei (p. vannamei). shrimp given the sg supplemented diet (0.5, 1 and 2% w/w) for 7 days showed enhanced expression of the downstream signaling mediator of lipopolysaccharide and β-1,3-glucan binding protein (lgbp) and immune related genes including p-nf-κb, imd, ikkβ and ikkε, antimicrobial peptide pen-4, propo-i and ii. following immersion with vibrio parahaemolyticus ...201728442419
three new piscidins from orange-spotted grouper (epinephelus coioides): phylogeny, expression and functional characterization.the present study reports the identification, and characterization of three new putative piscidin paralogues, ecpis-2, ecpis-3 and ecpis-4, from orange-spotted grouper (epinephelus coioides). the cdna of the three piscidins with the 207, 216, and 231 nt open reading frame encoded respectively a 68-, 71-, and 76-amino acid preprotein consisting of the predicted signal peptide, and putative mature peptide and prodomain. the phylogenetic analysis indicated that multiple piscidin paralogues in one f ...201728433718
acute hepatopancreatic necrosis disease-causing vibrio parahaemolyticus strains maintain an antibacterial type vi secretion system with versatile effector repertoires.acute hepatopancreatic necrosis disease (ahpnd) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. ahpnd is caused by toxic strains of vibrio parahaemolyticus that have acquired a "selfish plasmid" encoding the deadly binary toxins pira(vp)/pirb(vp) to better understand the repertoire of virulence factors in ahpnd-causing v. parahaemolyticus, we conducted a comparative analysis using the genome sequences of the clinical strain rimd2210633 and of environmenta ...201728432099
recombinant pira-like toxin protects shrimp against challenge with vibrio parahaemolyticus, the aetiological agent of acute hepatopancreatic necrosis disease. 201728422302
plasmid dynamics in vibrio parahaemolyticus strains related to shrimp acute hepatopancreatic necrosis syndrome (ahpns).vibrio parahaemolyticus is a causative agent of acute hapatopancreatic necrosis syndrome (ahpns) which causes early mortality in white shrimp. emergence of ahpns has caused tremendous economic loss for aquaculture industry particularly in asia since 2010. previous studies reported that strains causing ahpns harbor a 69-kb plasmid with possession of virulence genes, pira and pirb. however, genetic variation of the 69-kb plasmid among ahpns related strains has not been investigated. this study aim ...201728404482
antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium bacillus subtilis mtcc 10403.brown seaweed anthophycus longifolius (turner) kützing (family sargassaceae) associated heterotrophic bacterium bacillus subtilis mtcc 10403 was found to be a potent isolate with broad range of antibacterial activity against important perceptive food pathogens vibrio parahaemolyticus, v. vulnificus, and aeromonas hydrophila. this bacterium was positive for polyketide synthetase gene (kc589397), and therefore, was selected to bioprospect specialized metabolites bearing polyketide backbone. bioact ...201728704687
antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from ocimum basilicum var. thyrsiflora leaves.fourteen fungal endophytes were isolated from the ocimum basilicum var. thyrsiflora leaves collected from northern thailand. eight genera were identified including aspergillus, ascochyta, nigrospora, blastomyces, colletotrichum, exidia, clitopilus, and nomuraea. the antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus cereus, escherichia coli, klebsiella ...201728698913
identification and growth optimization of a marine bacillus dk1-sa11 having potential of producing broad spectrum antimicrobial compounds.control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. marine bacteria are an enormous source of bio-controlling agents. the aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain dk1-sa11 isolated from qingdao bay of china yellow sea. microscopic characterization, api® 20e and 50 chb kit base carbohy ...201728653930
antibacterial and antifungal activities of spices.infectious diseases caused by pathogens and food poisoning caused by spoilage microorganisms are threatening human health all over the world. the efficacies of some antimicrobial agents, which are currently used to extend shelf-life and increase the safety of food products in food industry and to inhibit disease-causing microorganisms in medicine, have been weakened by microbial resistance. therefore, new antimicrobial agents that could overcome this resistance need to be discovered. many spices ...201728621716
development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens.bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ...201728505584
seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium.seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ...201728733150
preparation, characterization and antibacterial activity of chitosan and phosphorylated chitosan from cuttlebone of sepia kobiensis (hoyle, 1885).chitosan is a commercially available derivative of chitin that has been extensively studied for its antimicrobial properties. in order to improve the water solubility and its biological activity, the chemical modification or derivatisation is attempted. in the present investigation, the chitosan prepared from the cuttlebone of sepia kobiensis was being chemically modified by reacting it with orthophosphoric acid so as to obtain phosphorylated chitosan. then the chitosan and phosphorylated chitos ...201628507912
differential proteomics to explore the inhibitory effects of acidic, slightly acidic electrolysed water and sodium hypochlorite solution on vibrio parahaemolyticus.slightly acidic electrolysed water (slaew) and acidic electrolysed water (aew) have been demonstrated to effectively inactivate food-borne pathogens. however, the underlying mechanism of inactivation remains unknown. therefore, in this study, a differential proteomic platform was used to investigate the bactericidal mechanism of slaew, aew, and sodium hypochlorite (naocl) solutions against vibrio parahaemolyticus. the upregulated proteins after slaew, aew, and naocl treatments were identified as ...201626471589
halobacteriovorax, an underestimated predator on bacteria: potential impact relative to viruses on bacterial mortality.predation on bacteria and accompanying mortality are important mechanisms in controlling bacterial populations and recycling of nutrients through the microbial loop. the agents most investigated and seen as responsible for bacterial mortality are viruses and protists. however, a body of evidence suggests that predatory bacteria such as the halobacteriovorax (formerly bacteriovorax), a bdellovibrio-like organism, contribute substantially to bacterial death. until now, conclusive evidence has been ...201626251870
inactivation of vibrio parahaemolyticus by antimicrobial photodynamic technology using methylene blue.vibrio parahaemolyticus is the leading causative pathogen of gastroenteritis often related to contaminated seafood. photodynamic inactivation has been recently proposed as a strategy for killing cells and viruses. the objective of this study was to verify the bactericidal effects caused by photodynamic inactivation using methylene blue (mb) over v. parahaemolyticus via flow cytometry, agarose gel electrophoresis and sodium dodecyl sulfate polyacrylamide gel electrophoresis. vibrio parahaemolytic ...201625989459
detection of acute hepatopancreatic necrosis disease strain of vibrio parahaemolyticus using loop-mediated isothermal amplification. 201625988949
the first report of vibrio parahaemolyticus strain o10:k60 in japan, a new combination of o and k serotypes isolated from a patient with gastroenteritis.vibrio parahaemolyticus is an important pathogen that causes gastroenteritis in humans, generally associated with the consumption of contaminated seafood, particularly raw shellfish. there are many serotypes in v. parahaemolyticus resulting from a combination of o and k antigens. among them, o3:k6 and their variants, which represent the pandemic clone, are the most widespread strains worldwide. in this study, we examined v. parahaemolyticus isolated from a gastroenteritis patient's stool at a ho ...201625971326
identification of immune response-related genes and signalling pathways in spleen of vibrio parahaemolyticus-infected epinephelus fuscoguttatus (forskal) by next-generation sequencing. 201625786532
centrifugal loop-mediated isothermal amplification microdevice for rapid, multiplex and colorimetric foodborne pathogen detection.we present a centrifugal microfluidic device which enables multiplex foodborne pathogen identification by loop-mediated isothermal amplification (lamp) and colorimetric detection using eriochrome black t (ebt). five identical structures were designed in the centrifugal microfluidic system to perform the genetic analysis of 25 pathogen samples in a high-throughput manner. the sequential loading and aliquoting of the lamp cocktail, the primer mixtures, and the dna sample solutions were accomplishe ...201626322592
draft genome sequences of one marine and one clinical vibrio parahaemolyticus strain, both isolated in sweden.vibrio parahaemolyticus is the leading bacterial pathogen associated with seafood consumption. here, we report the draft genome sequences of one marine and one clinical strain, both isolated in sweden. these sequences will inform future comparative analysis of v. parahaemolyticus in northern europe.201627789643
epidemiological investigation of a foodborne outbreak in spain associated with u.s. west coast genotypes of vibrio parahaemolyticus.we describe an outbreak of seafood-associated vibrio parahaemolyticus in galicia, spain in on 18th of august 2012 affecting 100 of the 114 passengers travelling on a food banquet cruise boat. epidemiological information from 65 people was available from follow-on interviews, of which 51 cases showed symptoms of illness. the food items identified through the questionnaires as the most probable source of the infections was shrimp. this product was unique in showing a statistically significant and ...201626848427
serology, virulence, antimicrobial susceptibility and molecular characteristics of clinical vibrio parahaemolyticus strains circulating in southeastern china from 2009 to 2013.vibrio parahaemolyticus is a leading cause of food-borne diarrhoea in coastal countries. although v. parahaemolyticus cases have been reported since 1950, they have been poorly documented. from july 2009 to june 2013, we collected 6951 faecal specimens for pathogen detection; v. parahaemolyticus strains were isolated from 563 specimens (8.1%). we then analysed the characteristics of the 501 v. parahaemolyticus strains that were isolated as the sole pathogen. twenty-one serotypes were identified ...201626597222
a label-free multi-functionalized graphene oxide based electrochemiluminscence immunosensor for ultrasensitive and rapid detection of vibrio parahaemolyticus in seawater and seafood.a label-free electrochemiluminescence (ecl) immunosensor for ultrasensitive and rapid detection of marine pathogenic bacterium vibrio parahaemolyticus (vp) in seawater and seafood was developed based on multi-functionalized graphene oxide, which was prepared with n-(4-aminobutyl)-n-ethylisoluminol (abei) and vp antibody (anti-vp) simultaneously immobilized on the surface of magnetic graphene oxide (nanofe3o4@go). abei and anti-vp acted as the electrochemiluminophore and the capture device for vp ...201626592599
genetic diversity of vibrio parahaemolyticus strains isolated from farmed pacific white shrimp and ambient pond water affected by acute hepatopancreatic necrosis disease outbreak in thailand.acute hepatopancreatic necrosis disease (ahpnd) is an emerging shrimp disease that causes massive die-offs in farmed shrimps. recent outbreaks of ahpnd in asia have been causing great losses for shrimp culture and have become a serious socioeconomic problem. the causative agent of ahpnd is vibrio parahaemolyticus, which is typically known to cause food-borne gastroenteritis in humans. however, there have been few reports of the epidemiology of v. parahaemolyticus ahpnd strains, and the genetic r ...201626590959
detection, identification, and prevalence of pathogenic vibrio parahaemolyticus in fish and coastal environment in jordan.vibrio parahaemolyticus is widely distributed in the marine environments and considered the leading cause of human gastroenteritis in asian countries. a total of 150 marketed fish and 50 water and sediment samples from the gulf of aqaba were examined for the prevalence of pathogenic strains of v. parahaemolyticus. a total of 132 typical isolates obtained from the primary selective medium (thiosulfate-citrate bile salt sucrose agar) and showed positive biochemical properties were subjected to con ...201626554333
zebrafish as a useful model for zoonotic vibrio parahaemolyticus pathogenicity in fish and human.vibrio parahaemolyticus is an important aquatic zoonotic pathogen worldwide that causes vibriosis in many marine fish, and sepsis, gastroenteritis and wound infection in humans. however, the pathogenesis of different sources of v. parahaemolyticus is not fully understood. here, we examined the pathogenicity and histopathology of fish (v. parahaemolyticus 1.2164) and human (v. parahaemolyticus 17) strains in a zebrafish (danio rerio). we found that different infection routes resulted in different ...201626519599
prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from oysters in korea.vibrio parahaemolyticus is the most prevalent gastroenteritis-causing pathogen in korea and in some other asian countries. it is frequently found in oysters and other seafood. this study monitored changes in the prevalence of v. parahaemolyticus and environmental parameters in oyster aquaculture environments in korea. from june to october 2014, we tested oysters (crassostrea gigas) from shellfish-harvesting areas off the west coast of korea. these 71 isolates were the sum of 16 (22.5%), 19 (26.8 ...201626511256
trh (tdh-/trh+) gene analysis of clinical, environmental and food isolates of vibrio parahaemolyticus as a tool for investigating pathogenicity.sequencing analysis of the trh gene encoding the tdh-related haemolysin of tdh-/trh+ vibrio parahaemolyticus isolated in italy between 2002 and 2011 from clinical, environmental, and food samples revealed the presence of the trh2 variant in all isolates. the trh2 of the clinical isolate was 100% identical to other clinical tdh-/trh2 v. parahaemolyticus from europe. nucleotide and amino acid differences in the trh2 sequences of clinical isolates from italy and other countries allowed a differenti ...201626990408
variability in biofilm formation correlates with hydrophobicity and quorum sensing among vibrio parahaemolyticus isolates from food contact surfaces and the distribution of the genes involved in biofilm formation.vibrio parahaemolyticus is one of the leading foodborne pathogens causing seafood contamination. here, 22 v. parahaemolyticus strains were analyzed for biofilm formation to determine whether there is a correlation between biofilm formation and quorum sensing (qs), swimming motility, or hydrophobicity. the results indicate that the biofilm formation ability of v. parahaemolyticus is positively correlated with cell surface hydrophobicity, autoinducer (ai-2) production, and protease activity. field ...201626980068
genome characterization of a novel vibriophage vpkk5 (siphoviridae) specific to fish pathogenic strain of vibrio parahaemolyticus.vibrio parahaemolyticus has long been known pathogenic to shrimp but only recently it is also reported pathogenic to tropical cultured marine finfish. traditionally, bacterial diseases in aquaculture are often treated using synthetic antibiotics but concern due to side effects of these chemicals is elevating hence, new control strategies which are both environmental and consumer friendly, are urgently needed. one promising control strategy is the bacteriophage therapy. in this study, we report t ...201626960780
prevalence, characterization, and antibiotic susceptibility of vibrio parahaemolyticus isolated from retail aquatic products in north china.vibrio parahaemolyticus is a major foodborne pathogen, particularly in asian countries. increased occurrence of outbreaks of v. parahaemolyticus gastroenteritis in china indicates the need to evaluation of the prevalence of this pathogenic species. v. parahaemolyticus distribution in shellfish from the eastern coast of china has been reported previously. however, to date, the prevalence of v. parahaemolyticus in retail aquatic products in north china has not been determined. to investigate the p ...201626955871
essential oils of nigella sativa protects artemia from the pathogenic effect of vibrio parahaemolyticus dahv2.the anti-vibrio activity of essential oils (eos) of nine medicinal plants was tested against 28 vibrio spp. isolated from diseased fenneropenaeus indicus. eo of nigella sativa exhibited anti-vibrio activity against all vibrio spp. and greater inhibition was noted for the isolate v2 which was identified as vibrio parahaemolyticus dahv2. further, eo of n. sativa effectively inhibited v. parahaemolyticus dahv2 with an inhibition zone of 23.9mm at 101.2μgml(-1). moreover, eo of n. sativa revealed an ...201626945773
incidence rates and clinical symptoms of salmonella, vibrio parahaemolyticus, and shigella infections in china, 1998-2013.the etiological and clinical characteristics of patients with infectious diarrhea have changed during the last decade in shanghai.201626927452
single crd containing lectin from macrobrachium rosenbergii (mrlec) participates in innate immunity against pathogen infections.as a type of pattern-recognition proteins, lectins perform important functions in the innate immunity of crustaceans, including prawns. although several reports showed that c-type lectin domain family (clec) importantly functions in host-pathogen interactions, limited research has focused on clec in macrobrachium rosenbergii. in the present study, a new single crd containing clec (designated as mrlec) was reported in freshwater prawns, m. rosenbergii. the full-length cdna of mrlec consisted of 1 ...201626923244
editorial: ecology, virulence, and detection of pathogenic and pandemic vibrio parahaemolyticus. 201626909072
protective roles of katg-homologous genes against extrinsic peroxides in vibrio parahaemolyticus.the marine foodborne enteropathogen, vibrio parahaemolyticus, has four putative catalase genes. function of the katg-homologous genes, katg1(vpa0768) and katg2(vpa0453), was examined using gene deletion mutants, and compared with those of the kate-homologous genes, kate1(vpa1418) and kate2(vpa0305). bacterial growth of δkatg1 was significantly delayed in the presence of 200-300 μm h2o2, and such inhibition was enhanced when incubation temperature was lowered from 37°c to 22°c. in the stationary ...201626892020
variations of immune parameters in the lined seahorse hippocampus erectus after infection with enteritis pathogen of vibrio parahaemolyticus.enteritis has been increasingly recognized as one of the major obstacles for the lined seahorse hippocampus erectus mass culture success. in the present study, the intestinal bacteria strains of the lined seahorses h. erectus suffered from enteritis were isolated, then their pathogenicities were confirmed by artificial infection, and one pathogenic bacteria strain named ds3 was obtained. the median lethal dose (ld50) of strain ds3 for 10 days was determined. the seahorses with different infectio ...201626851568
responses of three very large inducible gtpases to bacterial and white spot syndrome virus challenges in the giant fresh water prawn macrobrachium rosenbergii.interferons (ifns) are cytokines secreted by cells in response to invasion by pathogens, such as viruses, bacteria, parasites, or tumor cells. very large inducible gtpases (vlig) are the latest ifn-inducible gtpase family to be discovered and are the largest known gtpases of any species. however, vlig proteins from invertebrates have yet to be characterized. in this study, three forms of vligs designated as mrvlig1, mrvlig2, and mrvlig3 were cloned from the giant fresh water prawn macrobrachium ...201626850335
sensor histidine kinase is a β-lactam receptor and induces resistance to β-lactam antibiotics.β-lactams disrupt bacterial cell wall synthesis, and these agents are the most widely used antibiotics. one of the principle mechanisms by which bacteria resist the action of β-lactams is by producing β-lactamases, enzymes that degrade β-lactams. in gram-negative bacteria, production of β-lactamases is often induced in response to the antibiotic-associated damage to the cell wall. here, we have identified a previously unidentified mechanism that governs β-lactamase production. in the gram-negati ...201626831117
pathogenicity of vibrio parahaemolyticus in different food matrices.the pathogenicity and virulence factors of vibrio parahaemolyticus in four food matrices--shrimp, freshwater fish, pork, and egg-fried rice--were compared by measuring the thermostable direct hemolysin activity and total hemolytic titer. significantly high thermostable direct hemolysin and also hemolytic titers (p < 0.05) were produced by v. parahaemolyticus in egg-fried rice > shrimp > freshwater fish > pork. filtrates of v. parahaemolyticus in shrimp given intraperitoneally induced marked live ...201626818990
biocompatibility and antibacterial activity of the adathoda vasica linn extract mediated silver nanoparticles.the aim of this study is to investigate the biocompatibility and anti-vibrio efficacy of green synthesized silver nanoparticles (agnps) using an aqueous leaf extract of adathoda vasica (a. vasica). the green synthesized silver nanoparticles were characterized by uv-vis, fourier transform infrared (ftir) spectroscopy, x-ray diffraction (xrd), transmission electron microscopy (tem), scanning electron microscopy (sem) and energy dispersive x-ray analysis (edx). a. vasica agnps showed significant an ...201626802519
rapid and sensitive detection of vibrio parahaemolyticus and vibrio vulnificus by multiple endonuclease restriction real-time loop-mediated isothermal amplification technique.vibrio parahaemolyticus and vibrio vulnificus are two marine seafood-borne pathogens causing severe illnesses in humans and aquatic animals. in this study, a recently developed novel multiple endonuclease restriction real-time loop-mediated isothermal amplification technology (mert-lamp) were successfully developed and evaluated for simultaneous detection of v. parahaemolyticus and v. vulnificus strains in only a single reaction. two mert-lamp primer sets were designed to specifically target tox ...201626797596
serogroup, virulence, and molecular traits of vibrio parahaemolyticus isolated from clinical and cockle sources in northeastern thailand.vibrio parahaemolyticus is responsible for seafood-borne gastroenteritis worldwide. isolates of v. parahaemolyticus from clinical samples (n=74) and cockles (anadara granosa) (n=74) in thailand were analyzed by serotyping, determination of virulence and related marker genes present, response to antimicrobial agents, and genetic relatedness. serological analysis revealed 31 different serotypes, 10 of which occurred among both clinical and cockle samples. the clinical isolates commonly included th ...201626773828
identification and pathogenicity of vibrio parahaemolyticus isolates and immune responses of penaeus (litopenaeus) vannamei (boone).five different vibrio parahaemolyticus strains (sh8, sh108, sh58, ah5 and gd10) isolated from the hepatopancreas of moribund shrimp in farms of mainland china were identified and capable of inducing massive mortality of penaeus (litopenaeus) vannamei. the immersion challenge results with five isolates indicated variance of virulence, while only gd10 caused massive sloughing of tubule epithelial cells which was recognized as the most significant symptom of ahpnd. differences in immune responses w ...201626763100
identification and characterization of mkk7 as an upstream activator of jnk in litopenaeus vannamei.mitogen-activated protein kinase kinase 7 (mkk7) is a key signal transduction regulator in c-jun n-terminal kinase (jnk) signaling pathway, which is involved in a wide range of physiological and pathological processes. in this study, we described the molecular cloning of a new member of mkk7 group from litopenaeus vannamei named as lvmkk7. the full-length cdna of lvmkk7 was 3093 bp in length, with an open reading frame (orf) of 1440bp encoding a putative protein of 479 amino acids. lvmkk7 contai ...201626707780
two distinct interferon-γ genes in tetraodon nigroviridis: functional analysis during vibrio parahaemolyticus infection.interferon gamma (ifnγ) is a th1 cytokine that plays a very important role in almost all phases of immune and inflammatory responses. in this study, we explored the functions of ifnγ1 and ifnγ2 of tetraodon nigroviridis. treating t. nigroviridis spleen and head kidney cells in vitro with recombinant t. nigroviridis ifnγ1 protein (rtn ifnγ1) or recombinant t. nigroviridis ifnγ2 protein (rtn ifnγ2) enhanced their nitric oxide responses. both rtn ifnγ1 and rtn ifnγ2 also induced the expression of i ...201626701668
draft genome sequence of pathogenic bacteria vibrio parahaemolyticus strain ba94c2, associated with acute hepatopancreatic necrosis disease isolate from south america.vibrio parahaemolyticus is a pathogenic bacteria which has been associated to the early mortality syndrome (ems) also known as hepatopancreatic necrosis disease (ahpnd) causing high mortality in shrimp farms. pathogenic strains contain two homologous genes related to insecticidal toxin genes, pira and pirb, these toxin genes are located on a plasmid contained within the bacteria. genomic sequences have allowed the finding of two strains with a divergent structure related to the geographic region ...201627570736
unhealthy sugars in bacterial cytotoxicity.bacterial type three secretion systems (t3sss) are central to virulence, but the processes stimulating their activation are unclear. in this issue of cell host & microbe, blondel et al. (2016) perform a genome-wide screen using crispr/cas technology to identify a role for cell surface sulfation and fucosylation in cytotoxicity mediated by vibrio parahaemolyticus t3sss.201627512898
identification and characterization of an 18.4kda antimicrobial truncation from shrimp litopenaeus vannamei hemocyanin upon vibrio parahaemolyticus infection.hemocyanin (hmc) is a multifunctional protein which plays many essential roles in invertebrate organism. recently more and more immune-related functions have been discovered on this protein. here the shrimp was infected with vibrio parahaemolyticus and the shrimp sera were analyzed by two-dimensional gel electrophoresis. totally 15 spots were identified as significantly up-regulated spots and further analyzed by maldi-tof/tof mass spectrometry (ms). four of them were identified as hmc derived tr ...201627506277
shrimp with knockdown of lvsocs2, a negative feedback loop regulator of jak/stat pathway in litopenaeus vannamei, exhibit enhanced resistance against wssv.jak/stat pathway is one of cytokine signaling pathways and mediates diversity immune responses to protect host from viral infection. in this study, lvsocs2, a member of suppressor of cytokine signaling (socs) families, has been cloned and identified from litopenaeus vannamei. the full length of lvsocs2 is 1601 bp, including an 1194 bp open reading frame (orf) coding for a putative protein of 397 amino acids with a calculated molecular weight of ∼42.3 kda. lvsocs2 expression was most abundant in ...201627497874
virulence and antibiotic and heavy metal resistance of vibrio parahaemolyticus isolated from crustaceans and shellfish in shanghai, china.vibrio parahaemolyticus can cause serious human seafoodborne gastroenteritis and even death. in this study, we isolated and characterized 208 v. parahaemolyticus strains from 10 species of commonly consumed crustaceans and shellfish available in fish markets in shanghai, people's republic of china, in 2014. most of these aquatic species had not been detected previously. the results revealed an extremely low occurrence of pathogenic v. parahaemolyticus carrying the toxin gene trh (1.9%). however, ...201627497124
leukocyte susceptibility and immune response against vibrio parahaemolyticus in totoaba macdonaldi.vibrio parahaemolyticus is a serious pathogen that affects aquaculture. nonetheless, to the best of our knowledge, no studies have focused on its immunological implications in totoaba macdonaldi. thus, the early immune response to v. parahaemolyticus in juveniles of totoaba was studied at 24 h post-infection with an in vivo study. in addition, changes in cellular innate immune parameters - phagocytosis, respiratory burst activity and viability (annexin v/propidium iodide) - were evaluated in vit ...201627486683
cloning of a big defensin gene and its response to vibrio parahaemolyticus challenge in the noble scallop chlamys nobilis (bivalve: pectinidae).the noble scallop chlamys nobilis has been an important marine cultured bivalve in the southern sea of china for decades. however, large-scale mortality events often occurred during the scallop' cultivation. as one of amps (antimicrobial peptides), big defensin is an important component of the innate immunity against pathogenic microorganisms in invertebrates. in order to investigate whether the big defensin can play a role in the immune defense against pathogenic microorganisms in noble scallop ...201627474446
feasibility of using multiple-locus variable-number tandem-repeat analysis for epidemiology study of vibrio parahaemolyticus infections.vibrio parahaemolyticus causes foodborne gastroenteritis, which is often associated with the consumption of raw or undercooked shellfish. molecular typing can provide critical information for detecting outbreaks and for source attribution. in this study, we describe the development and evaluation of an optimized multiple-locus variable-number tandem-repeat (vntr) analysis (mlva) for the characterization of v. parahaemolyticus isolates. the discriminatory power of mlva was compared to that of pul ...201627454001
crispr/cas9 screens reveal requirements for host cell sulfation and fucosylation in bacterial type iii secretion system-mediated cytotoxicity.type iii secretion systems (t3sss) inject bacterial effector proteins into host cells and underlie the virulence of many gram-negative pathogens. studies have illuminated bacterial factors required for t3ss function, but the required host processes remain largely undefined. we coupled crispr/cas9 genome editing technology with the cytotoxicity of two vibrio parahaemolyticus t3sss (t3ss1 and t3ss2) to identify human genome disruptions conferring resistance to t3ss-dependent cytotoxicity. we ident ...201627453484
comparison of the effects of environmental parameters on the growth variability of vibrio parahaemolyticus coupled with strain sources and genotypes analyses.microbial growth variability plays an important role on food safety risk assessment. in this study, the growth kinetic characteristics corresponding to maximum specific growth rate (μmax) of 50 v. parahaemolyticus isolates from different sources and genotypes were evaluated at different temperatures (10, 20, 30, and 37°c) and salinity (0.5, 3, 5, 7, and 9%) using the automated turbidimetric system bioscreen c. the results demonstrated that strain growth variability increased as the growth condit ...201627446034
pharmacokinetics and pharmacodynamics of sulfamethoxazole and trimethoprim in swimming crabs (portunus trituberculatus) and in vitro antibacterial activity against vibrio: pk/pd of smz-tmp in crabs and antibacterial activity against vibrio.serious bacterial pathogens have recently become a major cause of massive mortality in swimming crabs (portunus trituberculatus). in this study, the antibacterial activity against vibrio and the pharmacokinetics (pk) of sulfamethoxazole (smz)-trimethoprim (tmp) in crabs were estimated to explore the pharmacokinetics/pharmacodynamics (pk/pd) properties of the smz-tmp combination. the in vitro bacteriostatic activity and the anti-vibrio infection activity of the smz-tmp combination at various rati ...201627434870
tetraodon nigroviridis: a model of vibrio parahaemolyticus infection.vibriosis is the most common bacterial diseases and brings great economic loss on aquaculture. vibrio parahaemolyticus (v. parahaemolyticus), a gram-negative bacterium, has been identified as one main pathogens of vibriosis. the pathogenic mechanism of v. parahaemolyticus is not entirely clear now. in our study, a model of v. parahaemolyticus infection of green-spotted puffer fish (tetraodon nigroviridis) was established. t. nigroviridis were injected intraperitoneally (i.p.) with 200 μl of v. p ...201627426523
evaluation of toxa and vibrio parahaemolyticus lysate on humoral immune response and immune-related genes in pacific red snapper.immunogenicity of toxa and vibrio parahaemolyticus lysate was evaluated in a double immunostimulation scheme in pacific red snapper after v. parahaemolyticus infection. three groups of pacific red snapper were intraperitonealy (i.p.) injected with phosphate-buffered saline (pbs group), toxa of v. parahaemolyticus (toxa-vp group) or v. parahaemolyticus lysate (lysate-vp group) (first injection, day 1; second injection, day 7). fish were subsequently infected with live v. parahaemolyticus. humoral ...201627417232
sensitive detection of multiple pathogens using a single dna probe.a simple but promising electrochemical dna nanosensor was designed, constructed and applied to differentiate a few food-borne pathogens. the dna probe was initially designed to have a complementary region in vibrio parahaemolyticus (vp) genome and to make different hybridization patterns with other selected pathogens. the sensor was based on a screen printed carbon electrode (spce) modified with polylactide-stabilized gold nanoparticles (pla-aunps) and methylene blue (mb) was employed as the red ...201627414245
dangerous hitchhikers? evidence for potentially pathogenic vibrio spp. on microplastic particles.the taxonomic composition of biofilms on marine microplastics is widely unknown. recent sequencing results indicate that potentially pathogenic vibrio spp. might be present on floating microplastics. hence, these particles might function as vectors for the dispersal of pathogens. microplastics and water samples collected in the north and baltic sea were subjected to selective enrichment for pathogenic vibrio species. bacterial colonies were isolated from chromagar™vibrio and assigned to vibrio s ...201627411093
cloning and differential expression of a novel toll-like receptor gene in noble scallop chlamys nobilis with different total carotenoid content.to investigate whether toll like receptors (tlrs) genes do have an immune influence on noble scallop chlamys nobilis under pathogen stress, acute challenges lasting 48 h to vibrio parahaemolyticus, lipopolysaccharide (lps), polyinosinic polycytidylic acid (poly i:c), and pbs were conducted in two scallop stains of orange and brown with different carotenoids content. a novel toll-like receptor gene called cntlr-1 was cloned and its transcripts under different challenges were determined. meantime, ...201627403592
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