Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| immunological characterization and expression of lily-type lectin in response to environmental stress in turbot (scophthalmus maximus). | lectins are a superfamily of carbohydrate-binding proteins that are widely distributed throughout living organisms. in earlier work, we identified lily-type lectin (smltl) in the skin mucus of turbot scophthalmus maximus, and we characterized the protein in the present study. results from qrt-pcr indicated that smltl was expressed highly in skin, intestine and gill tissue. changes in smltl expression occurred in these tissues in response to environmental stressors including ciliate infection, hi ... | 2016 | 27542614 |
| dna vaccine encoding molecular chaperone groel of edwardsiella tarda confers protective efficacy against edwardsiellosis. | in the present study, molecular chaperone groel of edwardsiella tarda was found both react with flounder (paralichthys olivaceus) anti-recombinant groel (rgroel) and anti-e. tarda antibodies, and it was both detected in the outer membrane and the secretome. to evaluate the vaccine potential of groel, pci-neo-gfp-groel (pcg-groel) was prepared and used to vaccinate healthy flounder, and immune protective effects were investigated. the results showed that pcg-groel produced an rps of 60% following ... | 2016 | 27701022 |
| selecting stable reference genes for real-time quantitative pcr analysis in edwardsiella tarda. | edwardsiella tarda is a gram-negative pathogenic bacterium in aquaculture which can cause hemorrhagic septicemia in fish. many secreted proteins have been identified as virulent factors of e. tarda. while the virulent phenotype is based on the expression regulation of the virulent genes. it is important to understand the expression profile of the virulent genes. quantitative rt-pcr is one of the preferred methods for determining different gene expressions. it is important to select the most stab ... | 2016 | 27666991 |
| immunoglobulin tau heavy chain (igt) in flounder, paralichthys olivaceus: molecular cloning, characterization, and expression analyses. | immunoglobulin tau (igt) is a new teleost immunoglobulin isotype, and its potential function in adaptive immunity is not very clear. in the present study, the membrane-bound and secreted igt (migt and sigt) heavy chain genes were cloned for the first time and characterized in flounder (paralichthys olivaceus), and found the nucleic acid sequence were exactly same in the cτ1-cτ4 constant domains of migt and sigt, but different in variable regions and the c-terminus. the amino acid sequence of mig ... | 2016 | 27649168 |
| tolc plays a crucial role in immune protection conferred by edwardsiella tarda whole-cell vaccines. | although vaccines developed from live organisms have better efficacy than those developed from dead organisms, the mechanisms underlying this differential efficacy remain unexplored. in this study, we combined sub-immunoproteomics with immune challenge to investigate the action of the outer membrane proteome in the immune protection conferred by four edwardsiella tarda whole-cell vaccines prepared via different treatments and to identify protective immunogens that play a key role in this immune ... | 2016 | 27406266 |
| antimicrobial activity of essential oil of eucalyptus globulus against fish pathogenic bacteria. | the antibacterial activities of the essential oil of eucalyptus globulus (eoeg) was determined against 7 fish pathogenic bacteria (edwardsiella tarda, streptococcus iniae, s. parauberis, lactococcus garviae, vibrio harveyi, v. ichthyoenteri and photobacterium damselae) obtained from farmed olive flounder. the inhibitory activity was evaluated by three methods: disc diffusion method, minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc). according to the disc diffusi ... | 2016 | 27382376 |
| molecular cloning and expression analysis of a new lily-type lectin in the rock bream, oplegnathus fasciatus. | a new lily-type lectin rbltl was identified from rock bream (oplegnathus fasciatus) and its expression analysed. in this study, a new lily-type lectin gene (rbltl) was cloned from rock bream using expressed sequence tag (est) analysis. the full-length rbltl cdna was encoding a 117-amino acid protein. the deduced amino acid sequence of rbltl contained all of the conserved features crucial for its fundamental structure, including b-lectin domain and three d-mannose binding sites. rbltl mrna was pr ... | 2016 | 27345170 |
| distinct immune tones are established by lactococcus lactis bfe920 and lactobacillus plantarum fgl0001 in the gut of olive flounder (paralichthys olivaceus). | the immune tone is defined as an immunological state during which the readiness for immune response is potentiated. the establishment of immune tone in the gut of olive flounder (paralichthys olivaceus) was investigated by feeding lactococcus lactis bfe920 (ll) or lactobacillus plantarum fgl0001 (lp). ll-fed flounder showed significantly increased levels of regulatory genes (foxp3, il-10, and tgf-β1), cd18, and cd83 in the gut. in contrast, lp feeding drastically increased proinflammatory genes ... | 2016 | 27320869 |
| expression and role of gap junction protein connexin43 in immune challenge-induced extracellular atp release in japanese flounder (paralichthys olivaceus). | connexin43 (cx43) is the best characterized gap junction protein that allows the direct exchange of signaling molecules during cell-to-cell communications. the immunological functions and atp permeable properties of cx43 have been insensitively examined in mammals. the similar biological significance of cx43 in lower vertebrates, however, is not yet understood. in the present study we identified and characterized a cx43 ortholog (termed pocx43) from japanese flounder (paralichthys olivaceus) and ... | 2016 | 27291350 |
| computer-aided vaccine designing approach against fish pathogens edwardsiella tarda and flavobacterium columnare using bioinformatics softwares. | edwardsiella tarda and flavobacterium columnare are two important intracellular pathogenic bacteria that cause the infectious diseases edwardsiellosis and columnaris in wild and cultured fish. prediction of major histocompatibility complex (mhc) binding is an important issue in t-cell epitope prediction. in a healthy immune system, the t-cells must recognize epitopes and induce the immune response. in this study, t-cell epitopes were predicted by using in silico immunoinformatics approach with t ... | 2016 | 27284239 |
| edwardsiella tarda esae (orf19 protein) is required for the secretion of type iii substrates, and pathogenesis in fish. | type iii secretion system (t3ss) is a large macromolecular assembly found on the surface of many pathogenic gram-negative bacteria. edwardsiella tarda is an important gram-negative pathogen that employs t3ss to deliver effectors into host cells to facilitate its survival and replication. eseb, esec, and esed, when secreted, form a translocon complex esebcd on host membranes through which effectors are translocated. the orf19 gene (esae) of e. tarda is located upstream of esak, and downstream of ... | 2016 | 27283851 |
| esee of edwardsiella tarda augments secretion of translocon protein esec and expression of the escc-esee operon. | edwardsiella tarda is an important gram-negative pathogen that employs a type iii secretion system (t3ss) to deliver effectors into host cells to facilitate bacterial survival and replication. these effectors are translocated into host cells through a translocon complex composed of three secreted proteins, namely, eseb, esec, and esed. the secretion of eseb and esed requires a chaperone protein called escc, whereas the secretion of esec requires the chaperone esca. in this study, we identified a ... | 2016 | 27271743 |
| antigen uptake and expression of antigen presentation-related immune genes in flounder (paralichthys olivaceus) after vaccination with an inactivated edwardsiella tarda immersion vaccine, following hyperosmotic treatment. | antigen uptake is a critical process for activation of the immune system, and therefore the ability to enhance antigen uptake is a primary consideration in the development of an immersion vaccination of fish. in the present work, flounders (paralichthys olivaceus) were immersed in three hyperosmotic solutions with 40, 50 and 60‰ salinities, then transferred into seawater of normal salinity (i.e. 30‰) containing formalin-inactivated edwardsiella tarda for 30 min. the antigen uptake in vaccinated ... | 2016 | 27263114 |
| the serine protease autotransporter tsh contributes to the virulence of edwardsiella tarda. | the temperature-sensitive hemagglutinin (tsh), identified as serine protease autotransporters of the enterobacteriaceae (spates) proteins, is an important virulence factor for avian-pathogenic escherichia coli (apec) and uropathogenic e. coli. however, little is known about the role of tsh as a virulence factor in edwardsiella tarda, a severe fish pathogen. in this study, we characterized the tsh of e. tarda (named tshet) and examined its function and vaccine potential. tshet is composed of 1224 ... | 2016 | 27259829 |
| lactoferrin decreases the intestinal inflammation triggered by a soybean meal-based diet in zebrafish. | intestinal inflammation is a harmful condition in fish that can be triggered by the ingestion of soybean meal. due to the positive costs-benefits ratio of including soybean meal in farmed fish diets, identifying additives with intestinal anti-inflammatory effects could contribute to solving the issues caused by this plant protein. this study evaluated the effect of incorporating lactoferrin (lf) into a soybean meal-based diet on intestinal inflammation in zebrafish. larvae were fed with diets co ... | 2016 | 27247950 |
| nod2 in zebrafish functions in antibacterial and also antiviral responses via nf-κb, and also mda5, rig-i and mavs. | nod2/ripk2 signalling plays essential role in the modulation of innate and adaptive immunity in mammals. in this study, nod2 was functionally characterized in zebrafish (danio rerio), and its interaction with a receptor-interaction protein, ripk2, and rlrs such as mda5 and rig-i, as well as the adaptor, mavs was revealed in fish innate immunity. the expression of nod2 and ripk2 in zf4 cells has been constitutive and can be induced by the infection of edwardsiella tarda and svcv. the nod2 can sen ... | 2016 | 27235368 |
| intracellular translocation and localization of edwardsiella tarda type iii secretion system effector eseg in host cells. | edwardsiella tarda, an important fish pathogenic bacterium, could utilize type iii secretion system (t3ss) to transfer multiple effector proteins into host cells during infection. eseg was identified to be an e. tarda t3ss effector, which could be injected by t3ss into non-phagocytic cells. since e. tarda is a facultative intracellular pathogen that resides and replicates in macrophage, it is interesting to expand our knowledge about eseg translocation and localization within phagocytic cells. h ... | 2016 | 27208750 |
| a prebiotic role of ecklonia cava improves the mortality of edwardsiella tarda-infected zebrafish models via regulating the growth of lactic acid bacteria and pathogen bacteria. | in this study, the beneficial prebiotic roles of ecklonia cava (e. cava, ec) were evaluated on the growth of lactic acid bacteria (lab) and pathogen bacteria and the mortality of pathogen-bacteria infected zebrafish model. the result showed that the original e. cava (ec) led to the highest growth effects on three labs (lactobacillus brevis, l. brevis; lactobacillus pentosus, l. pentosus; lactobacillus plantarum; l. plantarum) and it was dose-dependent manners. also, ec, its celluclast enzymatic ... | 2016 | 27192145 |
| expression, secretion and bactericidal activity of type vi secretion system in vibrio anguillarum. | the type vi secretion system (t6ss) was recently shown to modulate quorum sensing and the stress response in vibrio anguillarum serotype o1 strain nb10. it is not known whether there is a functionally active t6ss in other serotypes of v. anguillarum. here, homologues to t6ss cluster vtsefgh and hemolysin-coregulated protein (hcp)-encoding genes were found to be prevalent and conserved in clinical isolates of v. anguillarum from fish, including four o1 and five non-o1 serotype strains. unexpected ... | 2016 | 27172981 |
| orf13 in the type iii secretion system gene cluster of edwardsiella tarda binds to the mammalian factor cugbp2. | the type iii secretion system (ttss) is essential for the intracellular replication of edwardsiella tarda in phagocytes of fish and mammals, and a hypothetical gene (orf13) located in the ttss gene cluster is required for intracellular replication and virulence of e. tarda. here, we show that under ttss-inducing conditions, the protein orf13 was secreted into culture supernatant. then, using a yeast 2-hybrid screen, we show that the mammalian factor cugbp2, which regulates apoptosis in breast ca ... | 2016 | 27137075 |
| immunomodulation of lactobacillus pentosus pl11 against edwardsiella tarda infection in the head kidney cells of the japanese eel (anguilla japonica). | wild and farm-raised fish can be simultaneously exposed to different types of pathogens in their habitats. hence, it is important to study their effects, whether isolated or in combination. therefore, the aim of this study was to evaluate the effects of lactobacillus pentosus pl11 on the transcription of specific cytokine genes related to immune response, using japanese eel macrophages as an in vitro model. head kidney leukocytes were isolated from japanese eels and cell viability was determined ... | 2016 | 27108377 |
| cloning and characterization of apoptosis-associated speck-like protein containing a card domain (asc) gene from japanese flounder paralichthys olivaceus. | apoptosis-associated speck-like protein containing a card domain (asc) is a critical adaptor molecule in multiple inflammasome protein complexes that mediate inflammation and host defense. however, few studies have been performed in lower vertebrates such as in teleost. here we identified and characterized a novel asc gene (namely poasc) from japanese flounder paralichthys olivaceus. the complete cdna sequence of poasc contains a 22 bp 5'-untranslated sequence, a 612 bp open reading frame, and a ... | 2016 | 27103005 |
| identification and characterization of atp-gated p2x2 receptor gene dominantly expressed in the japanese flounder (paralichthys olivaceus) head kidney macrophages. | p2x2 receptor (p2x2r) belongs to the family of purinergic receptors that have been shown to play important roles in regulating host innate immune response. although the immunologic significance of p2x2r has been studied in mammals, the presence and immune relevance of p2x2r in fish remains unclear. in this study we extended our previous observations by identifying and characterizing a p2x2r ortholog (termed pop2x2r) from japanese flounder (paralichthys olivaceus). quantitative real-time pcr anal ... | 2016 | 27103003 |
| the global regulatory effect of edwardsiella tarda fur on iron acquisition, stress resistance, and host infection: a proteomics-based interpretation. | ferric uptake regulator (fur) is an important transcriptional regulator of gram-negative bacteria. edwardsiella tarda is a severe fish bacterial pathogen with a broad host range that includes humans. in this study, we examined the regulatory function of fur in e. tarda via a proteomic approach. compared to the wild type tx01, the fur mutant tx01δfur exhibited (i) retarded growth, (ii) enhanced siderophore production, (iii) increased acid tolerance, which is in contrast to observations in other b ... | 2016 | 27102497 |
| identification of genes associated with the penetration activity of the human type of edwardsiella tarda edwgii through human colon epithelial cell monolayers. | edwardsiella tarda is a gram-negative pathogen with a broad host range including fish and humans. e. tarda causes gastrointestinal and extraintestinal infections in humans. in present study, the penetration activities of 22 strains of e. tarda, including 10 human isolates and 12 diseased fish isolates, through caco-2 cell monolayers were evaluated. all the human isolates exhibited penetration activity in contrast to the fish isolates, which did not. in order to identify genes responsible for pen ... | 2016 | 27057670 |
| the role of regulator eha in edwardsiella tarda pathogenesis and virulence gene transcription. | edwardsiella tarda is a pathogen with a broad host range that infects both animals and humans. eha is a new transcriptional regulator identified in et13, which is involved in the bacterial hemolytic activity. this study explored the effect of the eha in the pathogenesis of e. tarda and the transcriptional regulation of the bacterial virulence genes (esec, flic, pagc and fima). our results found that the virulence of the eha mutant was 2.5-fold less than the one of its wild et13 by ld50 in a muri ... | 2016 | 27038844 |
| thioredoxin domain-containing protein 12 from oplegnathus fasciatus: molecular characterization, expression against immune stimuli, and biological activities related to oxidative stress. | thioredoxin domain-containing protein 12 (txndc12) is a small, disulfide-containing protein that belongs to the thioredoxin (txn) superfamily. in the present study, we identified and characterized a txndc12-like gene, designated oftxndc12, from rock bream, oplegnathus fasciatus. oftxndc12 consists of seven exons interrupted by six introns. comparative genomic structural analysis revealed that the txndc12 of vertebrates is a structurally conserved gene. the coding sequence of oftxndc12 comprises ... | 2016 | 27026037 |
| histologic and molecular characterization of edwardsiella piscicida infection in largemouth bass (micropterus salmoides). | the genus edwardsiella is composed of a diverse group of facultative anaerobic, gram-negative bacteria that can produce disease in a wide variety of hosts, including birds, reptiles, mammals, and fish. our report describes the isolation and identification of edwardsiella piscicida associated with chronic mortality events in 2 separate captive largemouth bass (micropterus salmoides) populations in new york and florida. wet-mount biopsies of skin mucus, gill, kidney, and spleen from several affect ... | 2016 | 26951328 |
| a prebiotic effect of ecklonia cava on the growth and mortality of olive flounder infected with pathogenic bacteria. | olive flounder (paralichthys olivaceus), also known as the japanese flounder in japan, is one of the most important commercial marine finfish species cultured in korea and japan. the purpose of this study was to evaluate how a species of brown algae (ecklonia cava, e. cava) affects the growth rate of olive flounder and its immune response to pathogenic bacteria. first, the experimental fish were divided into four groups: the control group was fed the diet containing only 1.0% lactobacillus plant ... | 2016 | 26921543 |
| identification and molecular characterization of peroxiredoxin 6 from japanese eel (anguilla japonica) revealing its potent antioxidant properties and putative immune relevancy. | peroxiredoxins (prdx) are thiol specific antioxidant enzymes that play a pivotal role in cellular oxidative stress by reducing toxic peroxide compounds into nontoxic products. in this study, we identified and characterized a peroxiredoxin 6 counterpart from japanese eel (anguilla japonica) (ajprdx6) at molecular, transcriptional and protein level. the identified full-length coding sequence of ajprdx6 (669 bp) coded for a polypeptide of 223 aa residues (24.9 kda). deduced protein of ajprdx6 showe ... | 2016 | 26911410 |
| edwardsiella tarda endocarditis confirmed by indium-111 white blood cell scan: an unusual pathogen and diagnostic modality. | edwardsiella tarda is a freshwater marine member of the family enterobacteriaceae which often colonizes fish, lizards, snakes, and turtles but is an infrequent human pathogen. indium-111- ((111)in-) labeled white blood cell (wbc) scintigraphy is an imaging modality which has a wide range of reported sensitivity and specificity (from 60 to 100% and from 68 to 92%, resp.) for diagnosing acute and chronic infection. we describe a case of suspected e. tarda prosthetic aortic valve and mitral valve e ... | 2016 | 26885418 |
| identification and characterization of a novel nod-like receptor family card domain containing 3 gene in response to extracellular atp stimulation and its role in regulating lps-induced innate immune response in japanese flounder (paralichthys olivaceus) head kidney macrophages. | nucleotide oligomerization domain (nod)-like receptor (nlr) family with a caspase activation and recruitment domain (card) containing 3 (nlrc3) protein is an important cytosolic pattern recognition receptor that negatively regulates innate immune response in mammals. hitherto, the immunological significance of nlrc3 protein in fish remains largely uncharacterized. here we identified and characterized a novel nlrc3 gene (named ponlrc3) implicated in regulation of fish innate immunity from japanes ... | 2016 | 26820104 |
| antimicrobial response of galectin-1 from rock bream oplegnathus fasciatus: molecular, transcriptional, and biological characterization. | in this study, we describe the identification and characterization of a proto type galectin, galectin-1, from rock bream oplegnathus fasciatus (ofgal-1). galectins are evolutionarily conserved carbohydrate binding lectins that show a wide range of functions related to development and immune physiology. they have been identified as pattern recognition receptors of innate immune system that recognize a broad range of microbes. ofgal-1 cdna comprised of 993 bp with an open reading frame of 408 bp t ... | 2016 | 26792759 |
| identification of endonuclease domain-containing 1 gene in japanese flounder paralichthys olivaceus. | the mrna level of the endonuclease domain-containing 1 gene (jf_endod1) in japanese flounder paralichthys olivaceus kidney was significantly increased after injection of formalin-killed bacteria cells (fkc) in the previous microarray study. endod1 is a member of the dna/rna non-specific nucleases family, and its role in fish immunity has not been reported. the open reading frame of jf_endod1 cdna was 912 bp, encoding 303 amino acids. the first 27 amino acids were predicted to be a signal peptide ... | 2016 | 26784919 |
| edwardsiella tarda ompa encapsulated in chitosan nanoparticles shows superior protection over inactivated whole cell vaccine in orally vaccinated fringed-lipped peninsula carp (labeo fimbriatus). | the use of oral vaccination in finfish has lagged behind injectable vaccines for a long time as oral vaccines fall short of injection vaccines in conferring protective immunity. biodegradable polymeric nanoparticles (nps) have shown potential to serve as antigen delivery systems for oral vaccines. in this study the recombinant outer membrane protein a (rompa) of edwardsiella tarda was encapsulated in chitosan nps (np-rompa) and used for oral vaccination of labeo fimbriatus. the rompa purity was ... | 2016 | 27827990 |
| temperature-dependent regulation of gene expression in japanese flounder paralichthys olivaceus kidney after edwardsiella tarda formalin-killed cells. | temperature affects the activities of the immune system and the susceptibility of fish to pathogens. to investigate the modulation of temperature on immune related gene expression in formalin-killed cells (fkc) of edwardsiella tarda-injected japanese flounder paralichthys olivaceus, fish reared at 15 or 22 °c were injected with fkc of e. tarda. the up-regulation of immune related genes was detected in fkc-injected fish at both temperatures by qpcr. the mrna expression of ifnγ was highly up-regul ... | 2016 | 27815208 |
| the protection effect of leap-2 on the mudskipper (boleophthalmus pectinirostris) against edwardsiella tarda infection is associated with its immunomodulatory activity on monocytes/macrophages. | liver-expressed antimicrobial peptide 2 (leap-2) is a cationic peptide that plays an important role in the host's innate immune system. however, the mechanism by which leap-2 modulates/regulates the host defense against pathogens remains largely unknown. in this study, we identified a cdna sequence encoding leap-2 homolog (bpleap-2) in the mudskipper, boleophthalmus pectinirostris. sequence analysis revealed that bpleap-2 belonged to the fish leap-2a cluster and that it was closely related to ay ... | 2016 | 27765699 |
| eha, a regulator of edwardsiella tarda, required for resistance to oxidative stress in macrophages. | edwardsiella tarda is distributed widely in a variety of hosts. eha has recently been found to be its virulence regulator. in order to explore the mechanism of its regulation, we investigated the survival rates of wild type strain et13, and its eha mutant and complemented strains in raw264.7 macrophages under light microscopic observation as well as by counting bacterial cfus on the plates. all of the different strains could live within the macrophages; however, the intracellular numbers of the ... | 2016 | 27511959 |
| transgenic overexpression of baff regulates the expression of immune-related genes in zebrafish, danio rerio. | the b-cell activating factor (baff) is a member of tumour necrosis factor (tnf) superfamily that specifically regulates b lymphocyte proliferation and survival. excess baff leads to overproduction of antibodies for secretion, anti-dsdna antibodies and a lupus-like syndrome in mice. to investigate whether transgenic overexpression of the zebrafish baff leads to immunoglobulin changes and/or early maturing of the immune system, a tol2-gfp-2a-baff/his recombinant plasmid was constructed by insertin ... | 2016 | 27994173 |
| comparison of the effects of dietary single and multi-probiotics on growth, non-specific immune responses and disease resistance in starry flounder, platichthys stellatus. | an 8-week feeding trial was conducted to evaluate the effects of dietary probiotics on growth performance and non-specific immune responses in starry flounder, platichthys stellatus. fish averaging 46.5 ± 0.65 g (mean ± sd) were fed one of the six experimental diets; one control (cont), and five other diets were prepared by supplementing single-probiotics 1 (bacillus subtilis; sp1, 2 × 10(9) cfu kg(-1) diet), single-probiotics 2 (bacillus licheniformis; sp2, 2 × 10(9) cfu kg(-1) diet), multi-pro ... | 2016 | 27818339 |
| essential oils to control ichthyophthiriasis in pacu, piaractus mesopotamicus (holmberg): special emphasis on treatment with melaleuca alternifolia. | in vitro effect of the melaleuca alternifolia, lavandula angustifolia and mentha piperita essential oils (eos) against ichthyophthirius multifiliis and in vivo effect of m. alternifolia for treating ichthyophthiriasis in one of the most important south american fish, piaractus mesopotamicus (holmberg), were evaluated. the in vitro test consisted of three eos, each at concentrations of 57 μl l(-1) , 114 μl l (-1) , 227 μl l(-1) and 455 μl l (-1) , which were assessed once an hour for 4 h in micro ... | 2016 | 26776242 |
| design and evaluation of an edwardsiella tarda dna vaccine co-encoding antigenic and adjuvant peptide. | edwardsiella tarda is associated with edwardsiellosis in cultured fish, resulting in heavy losses in aquaculture. so far, different types of vaccine have been attempted against e. tarda. in this study, an optimized eukaryotic expression plasmid was developed and an optimized dna vaccine co-encoding antigenic and adjuvant peptide using a bicistronic expression system was designed. as a result, a modified plasmid harbored cytomegalovirus (cmv) promoter attached with r region of long terminal repea ... | 2016 | 27765700 |
| polysaccharides from marine macroalga, padina gymnospora improve the nonspecific and specific immune responses of cyprinus carpio and protect it from different pathogens. | immunostimulation by plant-derived compounds presents a fascinating alternative to vaccines and antibiotics in aquaculture. fish farmers are longing for immunostimulants that activate both specific and nonspecific immune responses of fish and protect fishes from all possible infections. in this study, we observed that polysaccharide fraction from marine macroalga, padina gymnospora stimulated the immune response of common carp cyprinus carpio (filed for patent, indian patent no. 201641027311 dat ... | 2016 | 27633680 |
| analysis of the antibacterial effect of an edwardsiella tarda luxs inhibitor. | luxs/ai-2 quorum sensing is involved in the virulence of many bacterial pathogens, including the fish pathogen edwardsiella tarda. in a previous study, we identified a small peptide, 5906, which inhibits e. tarda luxs activity by binding specifically to luxs in a manner that probably prevents the formation of functional luxs homodimer. in the present study, using japanese flounder as the experimental animal, we analyzed the antibacterial effect of 5906 produced by dh5α/p5906 (an escherichia coli ... | 2016 | 26848432 |
| near-drowning-associated pneumonia with bacteremia caused by coinfection with methicillin-susceptible staphylococcus aureus and edwardsiella tarda in a healthy white man: a case report. | edwardsiella tarda is an enterobacteriaceae found in aquatic environments. extraintestinal infections caused by edwardsiella tarda in humans are rare and occur in the presence of some risk factors. as far as we know, this is the first case of near-drowning-associated pneumonia with bacteremia caused by coinfection with methicillin-susceptible staphylococcus aureus and edwardsiella tarda in a healthy patient. | 2016 | 27423181 |
| comment on the method of determining the trapped volume of plasma after centrifugation, based on the correlation between the trapped volume and the electrical conductivity. | 2016 | 4964894 | |
| the particulate graft in tumor surgery. | 2016 | 4942608 | |
| [racemization during peptide synthesis]. | 2016 | 4881545 | |
| [bibliographic tudy of nursing. present publications in nursing. 2]. | 2016 | 4877474 | |
| toxoplasma testing. | 2016 | 4739210 | |
| intramacrophage infection reinforces the virulence of edwardsiella tarda. | edwardsiella tarda is an important pathogenic bacterium that can replicate in macrophages. however, how the intramacrophage infection process affects the virulence of this bacterium is essentially unknown. here, we show that e. tarda replicates and induces a caspase-1-dependent cell pyroptosis in a murine macrophage model. via pyroptosis, intracellular e. tarda escapes to the extracellular milieu, forming a unique bacterial population. being different from the bacteria cultured alone, this uniqu ... | 2016 | 26953340 |
| edwardsiella tarda-induced inhibition of apoptosis: a strategy for intracellular survival. | edwardsiella tarda is a gram-negative bacterial pathogen that can infect a wide range of freshwater and marine fish. one salient feature of e. tarda is the ability to survive and replicate in various host cells. in this study, we observed that e. tarda replicated robustly in the zebrafish cell line zf4, and that e. tarda-infected cells exhibited no detectable signs of apoptosis. global transcriptome analysis and quantitative real-time rt-pcr revealed that e. tarda infection generally significant ... | 2016 | 27471679 |
| locomotion and activity phasing of some medium-sized mammals. | 2016 | 4996451 | |
| selection of donor for cardiac transplant. | 2016 | 4866697 | |
| comparative growth of the thermal algae cyanidium caldarium on nitrate and ammonia at different temperatures. | 2016 | 5072724 | |
| the pragmatic utilization of the p-k phenomenon. | 2016 | 5039530 | |
| distortion produced by pinhole collimators. | 2016 | 5056700 | |
| metabolic and hormonal patterns after three days of total fasting in 27 obese and nonobese subjects. | 2016 | 5051804 | |
| [clinical attempt to prevent post-traumatic epilepsy in children]. | 2016 | 4976100 | |
| diverticula of the rectum. | 2016 | 4972827 | |
| hair transplantation. | 2016 | 4887148 | |
| the elbow in arthrogryposis. | 2016 | 4766189 | |
| [method of determining residues of herbicides and their metabolites in nutritional products by thin-layer chromatography]. | 2016 | 4780621 | |
| [bibliography of psychology in japan]. | 2016 | 4926483 | |
| resection of the inferior vena cava above the renal veins. | 2016 | 5055980 | |
| gene dosage in a deletion for a nuclear-coded, mitochondrial enzyme. | 2016 | 4823666 | |
| [symptoms and recent findings in pseudo-hypoparathyroidism (author's transl)]. | 2016 | 4824010 | |
| upper extremity venous thrombosis with pulmonary embolism. a complication of excretory urography. | 2016 | 4859603 | |
| the fate and dosimetry of two lung scanning agents, 131 i maa and 99tcm ferrous hydroxide. | 2016 | 4720741 | |
| [in memoriam dr ante jamnicki]. | 2016 | 4943942 | |
| support for the free-end saddle partial denture. | 2016 | 4888880 | |
| jewish migration in its historical perspective. | 2016 | 4947356 | |
| [effect of 3,4-dihydroxyphenylalanine (levodopa) in hepatic coma]. | 2016 | 5037838 | |
| [clinico-genetic considerations on basilar impression. ii. the role of the hereditary factor in basilar impression]. | 2016 | 4978197 | |
| oral lesions. | 2016 | 4931041 | |
| [ideas of lenin and bulgarian medicine]. | 2016 | 4921010 | |
| dental changes in burkitt's lymphoma. | 2016 | 4718558 | |
| innervation of the hand: an electromyographic study. | 2016 | 4732560 | |
| [la fontaine and the apothecaries]. | 2016 | 4929537 | |
| editorial: the heavy chain diseases. | 2016 | 4844731 | |
| [testicular damage in elastorrhexis generalisata]. | 2016 | 5031134 | |
| planning for briggs. 1. | 2016 | 4844745 | |
| first description of programmed cell death10 (pdcd10) in rock bream (oplegnathus fasciatus): potential relations to the regulation of apoptosis by several pathogens. | in this study, we isolated and characterized programmed cell death10 (pdcd10), which is known to be related to apoptosis, from rock bream (oplegnathus fasciatus). the full-length rock bream pdcd10 (rbpdcd10) cdna (1459 bp) contains an open reading frame of 633 bp that encodes 210 amino acids. furthermore, multiple alignments revealed that the six of the α-helix bundles were well conserved among the other pdcd10 sequences tested. rbpdcd10 was significantly expressed in the liver, rbc (red blood c ... | 2016 | 26472617 |
| insights into the virulence-related genes of edwardsiella tarda isolated from turbot in europe: genetic homogeneity and evidence for vibrioferrin production. | edwardsiella tarda has long been known as a pathogen that causes severe economic losses in aquaculture industry. insights gained on e. tarda pathogenesis may prove useful in the development of new methods for the treatment of infections as well as preventive measures against future outbreaks. in this report, we have established the correlation between the presence of virulence genes, related with three aspects typically involved in bacterial pathogenesis (chondroitinase activity, quorum sensing ... | 2016 | 26096159 |
| effects of protein hydrolysates supplementation in low fish meal diets on growth performance, innate immunity and disease resistance of red sea bream pagrus major. | this study was conducted to evaluate the supplemental effects of three different types of protein hydrolysates in a low fish meal (fm) diet on growth performance, feed utilization, intestinal morphology, innate immunity and disease resistance of juvenile red sea bream. a fm-based diet was used as a high fish meal diet (hfm) and a low fish meal (lfm) diet was prepared by replacing 50% of fm by soy protein concentrate. three other diets were prepared by supplementing shrimp, tilapia or krill hydro ... | 2015 | 26074096 |
| generation of killed but metabolically active (kbma) edwardsiella tarda and evaluation of its potential as a protective vaccine. | a technology for inactivation of pathogens in human blood products by treatment with amotosalen hydrochloride (s-59) in combination with long wavelength ultraviolet light (uva) to decrease transfusion-mediated sepsis has been applied to make safe vaccines against human pathogenic bacteria, and the resultants were called killed but metabolically active (kbma) bacteria. in the present study, we first generated kbma edwardsiella tarda and evaluated its potential as a protective vaccine in olive flo ... | 2015 | 26074095 |
| metabolome strategy against edwardsiella tarda infection through glucose-enhanced metabolic modulation in tilapias. | edwardsiella tarda causes fish disease and great economic loss. however, metabolic strategy against the pathogen remains unexplored. in the present study, gc-ms based metabolomics was used to investigate the metabolic profile from tilapias infected by sublethal dose of e. tarda. the metabolic differences between the dying group and survival group allow the identification of key pathways and crucial metabolites during infections. more importantly, those metabolites may modulate the survival-relat ... | 2015 | 26057462 |
| full-genome sequence of a novel myovirus, gf-2, infecting edwardsiella tarda: comparison with other edwardsiella myoviral genomes. | edwardsiellosis, which is caused by edwardsiella tarda, a gram-negative bacterium, is one of the most serious infectious diseases in both marine and freshwater fish farms worldwide. previously, we reported the complete genome sequences of three e. tarda-lytic bacteriophages (two podoviruses and a myovirus), which were isolated from fish tissues and fish-rearing seawater. further genomic information regarding e. tarda phages is important for understanding phage-host interactions as well as for ap ... | 2015 | 26049743 |
| in vivo programmed gene expression based on artificial quorum networks. | the quorum sensing (qs) system, as a well-functioning population-dependent gene switch, has been widely applied in many gene circuits in synthetic biology. in our work, an efficient cell density-controlled expression system (qs) was established via engineering of the vibrio fischeri luxi-luxr quorum sensing system. in order to achieve in vivo programmed gene expression, a synthetic binary regulation circuit (araqs) was constructed by assembling multiple genetic components, including the quorum q ... | 2015 | 25979894 |
| comparative proteomic study of edwardsiella tarda strains with different degrees of virulence. | edwardsiella tarda is an enteric opportunistic pathogen that causes a great loss in aquaculture. this species has been described as a phenotypical homogeneous group; in contrast, serological studies and molecular typing revealed a wide heterogeneity. in this work, a proteomic study of differential expression of a virulent isolate from turbot cultured in the norwest of spain in comparison with an avirulent collection strain was performed in order to recognize proteins involved in virulence. one h ... | 2015 | 25979771 |
| comparative analysis of adaptive immune response after vaccine trials using live attenuated and formalin-killed cells of edwardsiella tarda in ginbuna crucian carp (carassius auratus langsdorfii). | edwardsiella tarda is an intracellular pathogen that causes edwardsiellosis in fish. although vaccine trials with formalin-killed cells (fkc) have been reported, the vaccinations failed in protect against e. tarda infection. on the other hand, a live attenuated vaccine strategy is effective against edwardsiellosis; however, the mechanism underlying its effectiveness in fish is unclear. in the present study, we compared the adaptive immune responses in fish vaccinated with fkcs and live attenuate ... | 2015 | 25959574 |
| first molecular characterisation and expression analysis of a teleost thioredoxin-interacting protein (txnip) gene from rock bream (oplegnathus fasciatus). | thioredoxin-interacting protein (txnip) is an important regulator of glucose metabolism that functions by inhibiting cellular glucose uptake. the full-length rock bream (oplegnathus fasciatus) txnip (rbtxnip) cdna (2499 bp) contains an open reading frame of 1188 bp encoding 396 amino acids. furthermore, multiple alignments showed that the arrestin domain was well conserved among the other txnip sequences tested. rbtxnip was predicted to contain a pxxp and ppxy motif. phylogenetic analysis indica ... | 2015 | 25934185 |
| a potential antioxidant enzyme belonging to the atypical 2-cys peroxiredoxin subfamily characterized from rock bream, oplegnathus fasciatus. | peroxiredoxins (prxs), a diverse family of antioxidant enzymes, exert their antioxidant function through which different peroxide species are detoxified. this study describes both structural and functional characterization of a mitochondrial prx identified in rock bream, oplegnathus fasciatus (rbprx5). the orf (573 bp) of rbprx5 encoded a protein of 190 amino acids (20 kda) containing a putative mitochondrial targeting sequence (residues 1-20) and a thioredoxin-2 motif (residues 31-190) and thre ... | 2015 | 25934084 |
| functional characterisation and expression analysis of recombinant serum amyloid p isoform 1 (rbsap1) from rock bream (oplegnathus fasciatus). | lectins are carbohydrate-binding proteins that play important roles in the recognition and elimination of pathogens via the innate immune system. pentraxins (ptx) are humoral lectins, which are multifunctional proteins in vertebrates. pentraxins can be divided into two groups based on their primary structure: short (c-reactive protein and serum amyloid p [sap]) and long pentraxins (ptx3 and neuronal pentraxins). previously, sap was shown to have ca(2+)-dependent binding specificity for various l ... | 2015 | 25917975 |
| edwardsiella tarda sip1: a serum-induced zinc metalloprotease that is essential to serum resistance and host infection. | edwardsiella tarda is a severe bacterial pathogen to a wide arrange of farmed fish. one salient virulent feature of e. tarda is a remarkable ability to survive in host serum. in this study, in order to identify e. tarda proteins involved in serum resistance, we conducted proteomic analysis to examine the extracellular protein profiles of tx01, a pathogenic e. tarda isolate, in response to serum treatment. five differentially expressed proteins were identified, one of which was a putative zinc pr ... | 2015 | 25899393 |
| edwardsiella tarda-regulated proteins in japanese flounder (paralichthys olivaceus): identification and evaluation of antibacterial potentials. | edwardsiella tarda is a severe aquaculture pathogen that infects a wide range of fish including japanese flounder (paralichthys olivaceus). in this study, proteomic analysis was conducted to examine flounder spleen proteins altered in expression during e. tarda infection. twenty differentially expressed proteins were identified, which belonged to five functional categories. five upregulated proteins, i.e. calmodulin (cam), cathepsin l (catl), calreticulin (crt), ferritin middle subunit (ferm), a ... | 2015 | 25896741 |
| fructose restores susceptibility of multidrug-resistant edwardsiella tarda to kanamycin. | edwardsiella tarda, the causative agent of edwardsiellosis, imposes medical challenges in both the clinic and aquaculture. the emergence of multidrug resistant strains makes antibiotic treatment impractical. the identification of molecules that facilitate or promote antibiotic efficacy is in high demand. in the present study, we aimed to identify small molecules whose abundance is correlated with kanamycin resistance in e. tarda by gas chromatography-mass spectrometry. we found that the abundanc ... | 2015 | 25675328 |
| exogenous alanine and/or glucose plus kanamycin kills antibiotic-resistant bacteria. | multidrug-resistant bacteria are an increasingly serious threat to human and animal health. however, novel drugs that can manage infections by multidrug-resistant bacteria have proved elusive. here we show that glucose and alanine abundances are greatly suppressed in kanamycin-resistant edwardsiella tarda by gc-ms-based metabolomics. exogenous alanine or glucose restores susceptibility of multidrug-resistant e. tarda to killing by kanamycin, demonstrating an approach to killing multidrug-resista ... | 2015 | 25651179 |
| real-time polymerase chain reaction assays for the detection and quantification of edwardsiella tarda, edwardsiella piscicida, and edwardsiella piscicida-like species in catfish tissues and pond water. | researchers have proposed the adoption of 3 distinct genetic taxa among bacteria previously classified as edwardsiella tarda; namely e. tarda, e. piscicida, and a taxon presently termed e. piscicida-like. individual real-time polymerase chain reaction (qpcr) assays were developed, based on published primers, for e. tarda, e. piscicida, and e. piscicida-like sp. to provide rapid quantitative confirmatory tests for these phenotypically ambiguous bacteria. the qpcr assays were shown to be repeatabl ... | 2015 | 25613040 |
| characterization of ampicillin-stressed proteomics and development of a direct method for detecting drug-binding proteins in edwardsiella tarda. | antibiotic-resistant edwardsiella tarda poses a severe challenge to aquaculture. an understanding for antibiotic-resistant mechanisms is crucial to control the disease. the present study characterizes e. tarda ampicillin-stressed proteome and shows the importance of energy metabolism including the tca cycle and glycolysis/gluconeogenesis in the antibiotic resistance. further combination with antibiotic measurement develops a new method for identification of antibiotic-binding proteins out of dif ... | 2015 | 25596334 |