Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps]. | to study the distribution and drug sensitivity test of bacteria of patients on chronic rhinosinusitis with or without nasal polyps. | 2016 | 27192905 |
| restrictive reporting of selected antimicrobial susceptibilities influences clinical prescribing. | cascade and restrictive reporting are useful strategies to enhance antibiotic stewardship programs. | 2016 | 25466592 |
| migration of dictyostelium discoideum to the chemoattractant folic acid. | dictyostelium discoideum can be grown axenically in a cultured media or in the presence of a natural food source, such as the bacterium klebsiella aerogenes (ka). here we describe the advantages and methods for growing d. discoideum on a bacterial lawn for several processes studied using this model system. when grown on a bacterial lawn, d. discoideum show positive chemotaxis towards folic acid (fa). while these vegetative cells are highly unpolarized, it has been shown that the signaling and cy ... | 2016 | 27271892 |
| dictyostelium discoideum as a surrogate host-microbe model for antivirulence screening in pseudomonas aeruginosa pao1. | the interest of the pharmaceutical industry in developing new antibiotics is decreasing, as established screening systems which identify compounds that kill or inhibit the growth of bacteria can no longer be used. consequently, antimicrobial screening using classical minimum inhibitory concentration (mic) measurements is becoming obsolete. the discovery of antimicrobial agents that specifically target a bacterial pathogen without affecting the host and its beneficial bacteria is a promising stra ... | 2016 | 27066943 |
| characterization and evaluation of antibacterial and antiproliferative activities of crude protein extracts isolated from the seed of ricinus communis in bangladesh. | ricinus communis (euphorbiaceae) has previously been reported to possess analgesic, antihistamine, antioxidant and anti-inflammatory activities. this study was designed for isolation, characterization and evaluation of antibacterial and anti-proliferative activities of r. communis seed protein. | 2016 | 27405609 |
| influence of nanomechanical stress induced by zno nanoparticles of different shapes on the viability of cells. | there is growing interest in nanostructures interacting with living organisms. however, there are still no general rules for the design of biocompatible nanodevices. here, we present a step towards understanding the interactions between nanostructures and living cells. we study the influence of nanomechanical stress induced by zinc oxide (zno) nanostructures of different shapes on the viability of both prokaryotic (gram-negative bacteria: escherichia coli and enterobacter aerogenes, and gram-pos ... | 2016 | 27074722 |
| one-pot synthesis and antimicrobial evaluation of novel 3-cyanopyridine derivatives of (-)-β-pinene. | a series of novel 3-cyanopyridine derivatives of (-)-β-pinene were designed and synthesized by one-pot four-component domino reactions. the targeted compounds were evaluated for their antimicrobial activity against four bacteria (klebsiella pneumoniae, enterobacter aerogenes, staphylococcus aureus, staphylococcus epidermidis) and a fungus (candida albicans). the results showed that most of the minimal inhibitory concentrations (mics) of these 3-cyanopyridine derivatives against the tested strain ... | 2016 | 26898336 |
| effect of nanocomposite packaging containing zno on growth of bacillus subtilis and enterobacter aerogenes. | recent advances in nanotechnology have opened new windows in active food packaging. nano-sized zno is an inexpensive material with potential antimicrobial properties. the aim of the present study is to evaluate the antibacterial effect of low density polyethylene (ldpe) containing zno nanoparticles on bacillus subtilis and enterobacter aerogenes. zno nanoparticles have been synthesized by facil molten salt method and have been characterized by x-ray diffraction (xrd), and scanning electron micro ... | 2016 | 26478403 |
| ndm-producing enterobacteriaceae in a chinese hospital, 2014-2015: identification of ndm-producing citrobacterwerkmanii and acquisition of blandm-1-carrying plasmid in vivo in a clinical escherichia coli isolate. | new delhi metallo-β-lactamase (ndm)-producing enterobacteriaceae (npe) shows prevalence in china. little is known about the mechanisms related to the spread of npe. recently, a total of 51 non-duplicated npe isolates were collected from a tertiary-care hospital in china and analysed for genetic relatedness by pfge, antimicrobial susceptibility by etest and sequence type by multilocus sequence typing. s1-pfge and southern blot analysis or pcr amplification were used for plasmid profiling. between ... | 2016 | 27667823 |
| mobilization of the salmonella genomic island sgi1 and the proteus genomic island pgi1 by the a/c2 plasmid carrying blatem-24 harboured by various clinical species of enterobacteriaceae. | the objective of this study was to transfer the salmonella genomic islands (gis) sgi1 and sgi1-v and the proteus gi pgi1-pmesc to clinical isolates of enterobacteriaceae harbouring an a/c2 plasmid. | 2016 | 27150396 |
| dissemination of the blakpc gene by clonal spread and horizontal gene transfer: comparative study of incidence and molecular mechanisms. | in addition to the global spread of the kpc-producing klebsiella pneumoniae (kpc-kp) clonal complex (cc)-258 clone, the blakpc gene may also spread by horizontal gene transfer (hgt), as suspected when more than one kpc-producing enterobacteriaceae (kpc-ent) species are isolated in a single patient. we aimed to characterize the incidence and molecular features of kpc-kp that were isolated alone (singular kpc-kp) versus kpc-kp that were isolated together with another kpc-ent species (joint kpc-kp) ... | 2016 | 27073266 |
| carbapenem-resistant enterobacter spp. in retail seafood imported from southeast asia to canada. | 2016 | 27533255 | |
| emergence of the plasmid-mediated mcr-1 gene in colistin-resistant enterobacter aerogenes and enterobacter cloacae. | 2016 | 26976876 | |
| synthesis of novel sulfonamide analogs containing sulfamerazine/sulfaguanidine and their biological activities. | sulfamerazine and sulfaguanidine are clenched with p-nitrobenzoyl chloride and the products obtained are reduced to naxs in ethanol-water. novel sulfonamides (6a-g and 9a-g) were synthesized by the reaction of these reduced products (4 and 8) with various sulfonyl chlorides (5a-g). the structures of these compounds were characterized using spectroscopic analysis (ir, (1)h-nmr, (13)c-nmr and hrms) technique. antimicrobial activity of sulfonamides (3, 4, 7, 8, 6a-g and 9a-g) was evaluated by the a ... | 2016 | 26327456 |
| 2-alkyl(aryl)-quinazolin-4(3h)-thiones, 2-r-(quinazolin-4(3h)-ylthio)carboxylic acids and amides: synthesis, molecular docking, antimicrobial and anticancer properties. | in this study, a series of novel 2-alkyl(aryl)-quinazolin-4(3h)-thiones, 2-r-(quinazolin-4(3h)-ylthio)carboxylic acids and amides were synthesized and evaluated for antimicrobial and anticancer activities. their structure was confirmed by elemental analysis and spectral data (ft-ir, lc-ms, (1)h-nmr). antimicrobial activity was tested in vitro against staphylococcus aureus, enterococcus faecalis, enterobacter aerogenes, pseudomonas aeruginosa, escherichia coli, klebsiella pneumonia, candida albic ... | 2016 | 25831026 |
| antibacterial and antimycotic activity of cotton fabrics, impregnated with silver and binary silver/copper nanoparticles. | effective method of obtaining of the bactericidal bandage materials by impregnation of cotton fabric by aqueous solutions of silver and copper salts followed by a certain regime of heat treatment is developed. the study of obtained materials by methods of optical spectroscopy, electron microscopy, and x-ray phase analysis showed the formation of crystalline silver nanoparticles (nps) and bimetallic ag/cu composites with the corresponding surface plasmon resonance (spr) bands in the absorption sp ... | 2016 | 26781286 |
| tss-emote, a refined protocol for a more complete and less biased global mapping of transcription start sites in bacterial pathogens. | bacteria rely on efficient gene regulatory mechanisms to switch between genetic programs when they are facing new environments. although this regulation can occur at many different levels, one of the key steps is the initiation of transcription. identification of the first nucleotide transcribed by the rna polymerase is therefore essential to understand the underlying regulatory processes, since this provides insight on promoter strength and binding sites for transcriptional regulators, and addi ... | 2016 | 27806702 |
| antibacterial and antibiotic-resistance modifying activity of the extracts and compounds from nauclea pobeguinii against gram-negative multi-drug resistant phenotypes. | multi-drug resistance of gram-negative bacteria constitutes a major obstacle in the antibacterial fight worldwide. the discovery of new and effective antimicrobials and/or resistance modulators is necessary to combat the spread of resistance or to reverse the multi-drug resistance. in this study, we investigated the antibacterial and antibiotic-resistance modifying activities against 29 gram-negative bacteria including multi-drug resistant (mdr) phenotypes of the methanol extracts from nauclea p ... | 2016 | 27386848 |
| emphysematous cholecystitis presenting as gas-forming liver abscess and pneumoperitoneum in a dialysis patient: a case report and review of the literature. | emphysematous cholecystitis is a rare variant of acute cholecystitis with a high mortality rate. the combination of emphysematous cholecystitis, liver abscess and pneumoperitoneum are even rarer. herein we present a case of emphysematous cholecystitis in a senile diabetic lady who had worsening hemodynamics while undergoing hemodialysis. | 2016 | 26932814 |
| homeostasis of metabolites in escherichia coli on transition from anaerobic to aerobic conditions and the transient secretion of pyruvate. | we have developed a method for rapid quenching of samples taken from chemostat cultures of escherichia coli that gives reproducible and reliable measurements of extracellular and intracellular metabolites by (1)h nmr and have applied it to study the major central metabolites during the transition from anaerobic to aerobic growth. almost all metabolites showed a gradual change after perturbation with air, consistent with immediate inhibition of pyruvate formate-lyase, dilution of overflow metabol ... | 2016 | 27853594 |
| emphysematous pyelitis and cystitis associated with vesicoureteral reflux in a diabetic dog. | a 12-year-old female dog with a 3-month history of poor response to diabetes treatment had an acute worsening of symptoms, including weakness and blindness. the dog had elevated blood glucose, alkaline phosphatase and urea concentration, hyposthenuria, glycosuria, hematuria, and pyuria. escherichia coli was isolated from the urine. radiographs and ultrasound examination showed that the dog had unilateral emphysematous pyelitis and concurrent cystitis associated with vesicoureteral reflux. | 2016 | 27041755 |
| prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in messalata central hospital, libya. | to investigate the prevalence of urinary tract infection among patients at messalata central hospital, libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials. | 2016 | 27569886 |
| antibacterial properties of tebipenem pivoxil tablet, a new oral carbapenem preparation against a variety of pathogenic bacteria in vitro and in vivo. | to systemically investigate the in vitro and in vivo antibacterial properties of tebipenem pivoxil tablet. in addition, acute toxicity of this preparation was also studied. | 2016 | 26751436 |
| isolation and characterization of a bacteriophage phieap-2 infecting multidrug resistant enterobacter aerogenes. | enterobacter aerogenes (enterobacteriaceae) is an important opportunistic pathogen that causes hospital-acquired pneumonia, bacteremia, and urinary tract infections. recently, multidrug-resistant e. aerogenes have been a public health problem. to develop an effective antimicrobial agent, bacteriophage phieap-2 was isolated from sewage and its genome was sequenced because of its ability to lyse the multidrug-resistant clinical e. aerogenes strain 3-sp. morphological observations suggested that th ... | 2016 | 27320081 |
| in vitro antimicrobial activity of a siderophore cephalosporin, s-649266, against enterobacteriaceae clinical isolates, including carbapenem-resistant strains. | s-649266 is a novel siderophore cephalosporin antibiotic with a catechol moiety on the 3-position side chain. two sets of clinical isolate collections were used to evaluate the antimicrobial activity of s-649266 against enterobacteriaceae. these sets included 617 global isolates collected between 2009 and 2011 and 233 β-lactamase-identified isolates, including 47 kpc-, 49 ndm-, 12 vim-, and 8 imp-producers. the mic90 values of s-649266 against the first set of escherichia coli, klebsiella pneumo ... | 2016 | 26574013 |
| comprehensive genome analysis of carbapenemase-producing enterobacter spp.: new insights into phylogeny, population structure, and resistance mechanisms. | knowledge regarding the genomic structure of enterobacter spp., the second most prevalent carbapenemase-producing enterobacteriaceae, remains limited. here we sequenced 97 clinical enterobacter species isolates that were both carbapenem susceptible and resistant from various geographic regions to decipher the molecular origins of carbapenem resistance and to understand the changing phylogeny of these emerging and drug-resistant pathogens. of the carbapenem-resistant isolates, 30 possessed blakpc ... | 2016 | 27965456 |
| transmission of enterobacter aerogenes septicemia in healthcare workers. | enterobacter aerogenes is recognized as an important bacterial pathogen in hospital-acquired infections. this report describes two unusual cases of septicemia caused by e. aerogenes in immunocompetent healthcare workers. e. aerogenes was isolated from blood cultures of the two patients experiencing septicemia. the clinical isolates were initially identified as e. aerogenes using a vitek ii automated system and 16s rrna sequence analysis, and; both isolates involved in the outbreak shared a commo ... | 2016 | 27610316 |
| impaired blood neutrophil function in the frequent exacerbator of chronic obstructive pulmonary disease: a proof-of-concept study. | the underlying biological mechanisms of the frequent exacerbator phenotype of copd remain unclear. we compared systemic neutrophil function in copd patients with or without frequent exacerbations. | 2016 | 27530251 |
| occurrence and antimicrobial susceptibility of enteric rods and pseudomonads isolated from the dental prostheses biofilm. | to determine the occurrence and the in vitro antimicrobial susceptibility of enteric rods and pseudomonads from the denture biofilm of 52 subjects at the center for dental specialties of sobral/ ceara, brazil. | 2016 | 27812616 |
| antimicrobial activity of croton macrostachyus stem bark extracts against several human pathogenic bacteria. | in kenya, leaves and roots from croton macrostachyus are used as a traditional medicine for infectious diseases such as typhoid and measles, but reports on possible antimicrobial activity of stem bark do not exist. in this study, the antibacterial and antifungal effects of methanol, ethyl acetate and butanol extracts, and purified lupeol of c. macrostachyus stem bark were determined against important human gram-negative pathogens escherichia coli, salmonella typhi, klebsiella pneumoniae, and ent ... | 2016 | 27293897 |
| nqrm (duf539) protein is required for maturation of bacterial na+-translocating nadh:quinone oxidoreductase. | na(+)-translocating nadh:quinone oxidoreductase (na(+)-nqr) catalyzes electron transfer from nadh to ubiquinone in the bacterial respiratory chain, coupled with na(+) translocation across the membrane. na(+)-nqr maturation involves covalent attachment of flavin mononucleotide (fmn) residues, catalyzed by flavin transferase encoded by the nqr-associated apbe gene. analysis of complete bacterial genomes has revealed another putative gene (duf539, here renamed nqrm) that usually follows the apbe ge ... | 2016 | 26644436 |
| carbapenem-resistant enterobacteriaceae: prevalence and risk factors in a single community-based hospital in korea. | carbapenemase-producing enterobacteriaceae (cpe) are gram-negative bacteria with increasing prevalence of infection worldwide. in korea, 25 cases of cpe isolates were reported by the korea centers for disease control and prevention in 2011. most cpe cases were detected mainly at tertiary referral hospitals. we analyzed the prevalence and risk factors for carbapenem-resistant enterobacteriaceae (cre) in a mid-sized community-based hospital in korea. | 2016 | 27659436 |
| bioprospecting of lipolytic microorganisms obtained from industrial effluents. | the lipases have ability to catalyze diverse reactions and are important in different biotechnological applications. the aim of this work was to isolate and characterize microorganisms that produce lipases, from different food industry effluents localized in pelotas, rs/brazil. bacteria were identified using gram stain and biochemical tests (vitek 2(r)). fungi were identified according to macro and micromorphology characteristics. the extracellular lipase production was evaluated using the rhoda ... | 2016 | 27556331 |
| insights into the global regulation of anaerobic metabolism for improved biohydrogen production. | to improve the biohydrogen yield in bacterial dark fermentation, a new approach of global anaerobic regulation was introduced. two cellular global regulators fnr and narp were overexpressed in two model organisms: facultatively anaerobic enterobacter aerogenes (ea) and strictly anaerobic clostridium paraputrificum (cp). the overexpression of fnr and narp greatly altered anaerobic metabolism and increased the hydrogen yield by 40%. metabolic analysis showed that the global regulation caused more ... | 2016 | 26476162 |
| longer contact times increase cross-contamination of enterobacter aerogenes from surfaces to food. | bacterial cross-contamination from surfaces to food can contribute to foodborne disease. the cross-contamination rate of enterobacter aerogenes on household surfaces was evaluated by using scenarios that differed by surface type, food type, contact time (<1, 5, 30, and 300 s), and inoculum matrix (tryptic soy broth or peptone buffer). the surfaces used were stainless steel, tile, wood, and carpet. the food types were watermelon, bread, bread with butter, and gummy candy. surfaces (25 cm(2)) were ... | 2016 | 27590818 |
| in vivo acquisition of carbapenemase gene blakpc-2 in multiple species of enterobacteriaceae through horizontal transfer of insertion sequence or plasmid. | objectives: current worldwide spread of carbapenem resistance in enterobacteriaceae constitutes a critical public health threat. this study aims to investigate how carbapenem resistance is acquired in enterobacteriaceae in patients during antimicrobial therapy. methods: clinical strains from the same anatomical site of the same patients that converted from carbapenem-susceptible to resistant during antimicrobial therapy and showed identical or similar pfge patterns were identified. the similarly ... | 2016 | 27818649 |
| ca(ii) binding regulates and dominates the reactivity of a transition-metal-ion-dependent diesterase from mycobacterium tuberculosis. | the diesterase rv0805 from mycobacterium tuberculosis is a dinuclear metallohydrolase that plays an important role in signal transduction by controlling the intracellular levels of cyclic nucleotides. as rv0805 is essential for mycobacterial growth it is a promising new target for the development of chemotherapeutics to treat tuberculosis. the in vivo metal-ion composition of rv0805 is subject to debate. here, we demonstrate that the active site accommodates two divalent transition metal ions wi ... | 2016 | 26662456 |
| isolation of kpc 3-producing enterobacter aerogenes in a patient colonized by mdr klebsiella pneumoniae. | we describe the interspecies transmission of the plasmid-mediated blakpc-3 gene, which confers carbapenem resistance, between clinically relevant gram-negative bacteria in a single patient. a kpc-3 producing enterobacter aerogenes was isolated from a hospitalized patient previously colonized and then infected by a klebsiella pneumoniae st101 carrying the blakpc-3 gene. the strains showed identical plasmids. since intense horizontal exchanges among bacteria can occur in the gut, clinicians should ... | 2016 | 27284988 |
| efflux pump blockers in gram-negative bacteria: the new generation of hydantoin based-modulators to improve antibiotic activity. | multidrug resistant (mdr) bacteria are an increasing health problem with the shortage of new active antibiotic agents. among effective mechanisms that contribute to the spread of mdr gram-negative bacteria are drug efflux pumps that expel clinically important antibiotic classes out of the cell. drug pumps are attractive targets to restore the susceptibility toward the expelled antibiotics by impairing their efflux activity. arylhydantoin derivatives were investigated for their potentiation of ac ... | 2016 | 27199950 |
| occurrence and analysis of irp2 virulence gene in isolates of klebsiella pneumoniae and enterobacter spp. from microbiota and hospital and community-acquired infections. | eighty-five isolates of klebsiella pneumoniae and enterobacter spp., originating from hospital- and community-acquired infections and from oropharyngeal and faecal microbiota from patients in recife-pe, brazil, were analyzed regarding the presence of irp2 gene. this is a yersinia typical gene involved in the synthesis of siderophore yersiniabactin. dna sequencing confirmed the identity of irp2 gene in five k. pneumoniae, five enterobacter aerogenes and one enterobacter amnigenus isolates. to our ... | 2016 | 27133266 |
| multidrug-resistant combined infections in a liver transplanted patient: case report. | we report a case of successfully treated multiple liver abscesses in a liver-transplanted patient, sustained by combined multidrug-resistant infections. two months after a liver transplant, a computed tomography scan revealed the presence of multiple abscesses in the liver graft. blood cultures and abscessual liver fluid were both positive for acquired colistin- and carbapenem- resistant klebsiella pneumoniae and an extended-spectrum of beta-lactamases-producing enterobacter aerogenes. the treat ... | 2016 | 27063638 |
| isolation of enterobacter aerogenes carrying blatem-1 and blakpc-3 genes recovered from a hospital intensive care unit. | enterobacter aerogenes has recently emerged as an important hospital pathogen. in this study, we showed the emergence of e. aerogenes isolates carrying the blakpc gene in patients colonized by carbapenem-resistant klebsiella pneumoniae strains. two multiresistant e. aerogenes isolates were recovered from bronchial aspirates of two patients hospitalized in the intensive care unit at the "santa maria della scaletta" hospital, imola. the antimicrobial susceptibility test showed the high resistance ... | 2016 | 27004836 |
| osteoconductive composite graft based on bacterial synthesized hydroxyapatite nanoparticles doped with different ions: from synthesis to in vivo studies. | to repair damaged bone tissues, osteoconductive bone graft substitutes are required for enhancement of the regenerative potential of osteoblast cells. nanostructured hydroxyapatite is a bioactive ceramic used for bone tissue engineering purposes. in this study, carbonate hydroxyapatite (cha) and zinc-magnesium substituted hydroxyapatite (zn-mg-ha) nanoparticles were synthesized via biomineralization method using enterobacter aerogenes. the structural phase composition and the morphology of the s ... | 2016 | 26956413 |
| genome sequencing of four multidrug-resistant enterobacter aerogenes isolates from hospitalized patients in brazil. | 2016 | 27833588 | |
| the melatonin-sensitive circadian clock of the enteric bacterium enterobacter aerogenes. | circadian clocks are fundamental properties of all eukaryotic organisms and at least some prokaryotic organisms. recent studies in our laboratory have shown that the gastrointestinal system contains a circadian clock that controls many, if not all, aspects of gastrointestinal function. we now report that at least one species of intestinal bacteria, enterobacter aerogenes, responds to the pineal and gastrointestinal hormone melatonin by an increase in swarming activity. this swarming behavior is ... | 2016 | 27387841 |
| physiological and molecular characteristics of carbapenem resistance in klebsiella pneumoniae and enterobacter aerogenes. | bacterial resistance is a growing concern in the nosocomial environment in which klebsiella pneumoniae and enterobacter aerogenes play an important role due to their opportunism and carbapenemase-production. this work aimed to evaluate physiological and molecular characteristics of carbapenem-resistant k. pneumoniae and e. aerogenes isolated in a brazilian tertiary hospital. | 2016 | 27367007 |
| enhancing hydrogen production of enterobacter aerogenes by heterologous expression of hydrogenase genes originated from synechocystis sp. | the hydrogenase genes (hoxefuyh) of synechocystis sp. pcc 6803 were cloned and heterologously expressed in enterobacter aerogenes atcc13408 for the first time in this study, and the hydrogen yield was significantly enhanced using the recombinant strain. a recombinant plasmid containing the gene in-frame with glutathione-s-transferase (gst) gene was transformed into e. aerogenes atcc13408 to produce a gst-fusion protein. sds-page and western blot analysis confirm the successful expression of the ... | 2016 | 27343449 |
| adequate hand washing and glove use are necessary to reduce cross-contamination from hands with high bacterial loads. | hand washing and glove use are the main methods for reducing bacterial cross-contamination from hands to ready-to-eat food in a food service setting. however, bacterial transfer from hands to gloves is poorly understood, as is the effect of different durations of soap rubbing on bacterial reduction. to assess bacterial transfer from hands to gloves and to compare bacterial transfer rates to food after different soap washing times and glove use, participants' hands were artificially contaminated ... | 2016 | 26818993 |
| mutational analysis of the hyc-operon determining the relationship between hydrogenase-3 and nadh pathway in enterobacter aerogenes. | in this study, the hydrogenase-3 gene cluster (hycdefgh) was isolated and identified from enterobacter aerogenes cctcc ab91102. all gene products were highly homologous to the reported bacterial hydrogenase-3 (hyd-3) proteins. the genes hyce, hycf, hycg encoding the subunits of hydrogenase-3 were targeted for genetic knockout to inhibit the fhl hydrogen production pathway via the red recombination system, generating three mutant strains ab91102-e (δhyce), ab91102-f (δhycf) and ab91102-g (δhycg). ... | 2016 | 26672442 |
| etiology of early onset septicemia among neonates at the university college hospital, ibadan, nigeria. | neonatal septicemia remains a major cause of newborn deaths in developing countries. its burden is further compounded by the emergence of multidrug-resistant pathogens, which is related to a lack of antibiotic protocols resulting in unrestricted use of antibiotics. the absence of reliable antibiotic sensitivity testing makes the formulation of antibiotic guidelines and judicious use of antibiotics difficult. this study sought to identify the current bacterial agents associated with early onset s ... | 2016 | 28036314 |
| trapping and viability of swimming bacteria in an optoelectric trap. | non-contact manipulation methods capable of trapping and transporting swimming bacteria can significantly aid in chemotaxis studies. however, high swimming speed makes the trapping of these organisms an inherently challenging task. we demonstrate that an optoelectric technique, rapid electrokinetic patterning (rep), can effectively trap and manipulate enterobacter aerogenes bacteria swimming at velocities greater than 20 μm s(-1). rep uses electro-orientation, laser-induced ac electrothermal flo ... | 2016 | 26891971 |
| enhanced dark hydrogen fermentation by addition of ferric oxide nanoparticles using enterobacter aerogenes. | ferric oxide nanoparticles (fonps) were used to facilitate dark hydrogen fermentation using enterobacter aerogenes. the hydrogen yield of glucose increased from 164.5±2.29 to 192.4±1.14ml/g when fonps concentration increased from 0 to 200mg/l. sem images of e. aerogenes demonstrated the existence of bacterial nanowire among cells, suggesting fonps served as electron conduits to enhance electron transfer. tem showed cellular internalization of fonps, indicating hydrogenase synthesis and activity ... | 2016 | 26890796 |
| molecular docking of glycine max and medicago truncatula ureases with urea; bioinformatics approaches. | urease (ec 3.5.1.5) is a nickel-dependent metalloenzyme catalyzing the hydrolysis of urea into ammonia and carbon dioxide. it is present in many bacteria, fungi, yeasts and plants. most species, with few exceptions, use nickel metalloenzyme urease to hydrolyze urea, which is one of the commonly used nitrogen fertilizer in plant growth thus its enzymatic hydrolysis possesses vital importance in agricultural practices. considering the essentiality and importance of urea and urease activity in most ... | 2016 | 26852122 |
| non-thiolate ligation of nickel by nucleotide-free ureg of klebsiella aerogenes. | nickel-dependent ureases are activated by a multiprotein complex that includes the gtpase ureg. prior studies showed that nucleotide-free ureg from klebsiella aerogenes is monomeric and binds one nickel or zinc ion with near-equivalent affinity using an undefined binding site, whereas nucleotide-free ureg from helicobacter pylori selectively binds one zinc ion per dimer via a universally conserved cys-pro-his motif in each protomer. iodoacetamide-treated k. aerogenes ureg was nearly unaffected i ... | 2016 | 28004186 |
| in vitro antimicrobial activity screening of xylaria hypoxylon. | fungi have a potential of using both as nutritive and medicinal food stuff. because of containing several therapeutic agents, they are reported to be used for hundreds of years to treat several diseases caused by bacteria, fungi, viruses and parasites. the aim of this study is to determine the in vitro antimicrobial activity of xylaria hypoxylon, which were collected from yomra, trabzon, turkey. | 2016 | 28852719 |
| determination of histamine in milkfish stick implicated in food-borne poisoning. | an incident of food-borne poisoning causing illness in 37 victims due to ingestion of fried fish sticks occurred in september 2014, in tainan city, southern taiwan. leftovers of the victims' fried fish sticks and 16 other raw fish stick samples from retail stores were collected and tested to determine the occurrence of histamine and histamine-forming bacteria. two suspected fried fish samples contained 86.6 mg/100 g and 235.0 mg/100 g histamine; levels that are greater than the potential hazard ... | 2016 | 28911410 |
| hygienic quality, adulteration of pork and histamine production by raoultella ornithinolytica in milkfish dumpling. | ten milkfish dumpling products purchased from retail stores in southern taiwan were collected to determine the occurrence of biogenic amines, histamine-forming bacteria, and adulteration of pork. this study showed the high contents of aerobic plate count (apc), total coliforms (tc) and escherichia coli in tested milkfish dumpling samples, whereas the average content of various biogenic amines in all tested samples was < 1.6 mg/100 g (< 0.05 to 1.54 mg/100 g). three histamine-producing bacterial ... | 2016 | 28911614 |
| formations of calcium carbonate minerals by bacteria and its multiple applications. | biomineralization is a naturally occurring process in living organisms. in this review, we discuss microbially induced calcium carbonate precipitation (micp) in detail. in the micp process, urease plays a major role in urea hydrolysis by a wide variety of microorganisms capable of producing high levels of urease. we also elaborate on the different polymorphs and the role of calcium in the formation of calcite crystal structures using various calcium sources. additionally, the environmental facto ... | 2016 | 27026942 |
| partial purification and characterization of a novel histidine decarboxylase from enterobacter aerogenes dl-1. | histidine decarboxylase (hdc) from enterobacter aerogenes dl-1 was purified in a three-step procedure involving ammonium sulfate precipitation, sephadex g-100, and deae-sepharose column chromatography. the partially purified enzyme showed a single protein band of 52.4 kd on sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). the optimum ph for hdc activity was 6.5, and the enzyme was stable between ph 4 and 8. enterobacter aerogenes hdc had optimal activity at 40°c and retained ... | 2015 | 25036745 |
| mutational and computational evidence that a nickel-transfer tunnel in ured is used for activation of klebsiella aerogenes urease. | nickel-containing urease from klebsiella aerogenes requires four accessory proteins for proper active site metalation. the metallochaperone uree delivers nickel to ureg, a gtpase that forms a ured/uref/ureg complex, which binds to urease apoprotein via ured. prior in silico analysis of the homologous, structurally characterized ureh/uref/ureg complex from helicobacter pylori identified a water tunnel originating at a likely nickel-binding motif in ureg, passing through uref, and exiting ureh, su ... | 2015 | 26401965 |
| reduction of urease activity by interaction with the flap covering the active site. | with the increasing appreciation for the human microbiome coupled with the global rise of antibiotic resistant organisms, it is imperative that new methods be developed to specifically target pathogens. to that end, a novel computational approach was devised to identify compounds that reduce the activity of urease, a medically important enzyme of helicobacter pylori, proteus mirabilis, and many other microorganisms. urease contains a flexible loop that covers its active site; glide was used to i ... | 2015 | 25594724 |
| purification and antibiofilm activity of ahl-lactonase from endophytic enterobacter aerogenes vt66. | the opportunistic pathogen pseudomonas aeruginosa uses biofilm lifestyle to resist antibiotic treatment. in our study, endophytic bacterium enterobacter aerogenes vt66 quenched the n-acyl homoserine lactone (ahl) molecules produced by p. aeruginosa pao1. the quorum quenching activity was attributed to the presence of ahl-lactonase. the ahl-lactonase was purified using column chromatography and purified ahl-lactonase was applied for the control of biofilm formation in p. aeruginosa pao1. the resu ... | 2015 | 26432367 |
| use of aiia gene amplification for ahl-lactonase production from endophytic bacterium enterobacter species. | ahl-lactonase has gained renewed interest due to biotechnological applications such as antiquorum sensing, antibiofilm strategies, biofouling, etc. in our study, the production of ahl-lactonase from endophytic bacteria enterobacter aerogenes vt66 was optimized by response surface methodology (rsm) using central composite design (ccd) for four different cultural conditions. the relative activity of ahl-lactonase was correlated with amplification of aiia homologous gene amplification with respect ... | 2015 | 25451751 |
| cytotoxic, antibacterial and antioxidant activities of extracts of the bark of melia azedarach (china berry). | nature provides a variety of drugs and medicinal agents derived from plants. this study was conducted to determine antimicrobial, antioxidant and cytotoxic activities of extracts of melia azedarach bark with methanol/water (9:1 v/v), chloroform, butanol, hexane, water and ethyl acetate. for the determination of the antimicrobial activities, the agar well diffusion method was employed. cytotoxicity was studied by brine shrimp lethality assay; antioxidant activities were measured using 1,1-dipheny ... | 2015 | 25426766 |
| effects of eliminating pyruvate node pathways and of coexpression of heterogeneous carboxylation enzymes on succinate production by enterobacter aerogenes. | lowering the ph in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs. newly isolated enterobacter aerogenes strain aj110637, a rapid carbon source assimilator under weakly acidic (ph 5.0) conditions, was selected as a platform for succinate production. our previous work showed that the δadhe/pck strain, developed from aj110637 with inactivated ethanol dehydrogenase and introduced actinobacillus succinogenes phosphoenolpyruvate carboxykinase ... | 2015 | 25416770 |
| authors reply to letter to the editor--in response to: "bhat ss, undrakonda v, mukhopadhyay c, parmar pv. outbreak of multidrug-resistant acute postoperative endophthalmitis due to enterobacter aerogenes". | 2015 | 25356851 | |
| in response to: "bhat ss, undrakonda v, mukhopadhyay c, parmar pv. outbreak of multidrug-resistant acute postoperative endophthalmitis due to enterobacter aerogenes.". | 2015 | 25356796 | |
| prevalence and characterization of high histamine-producing bacteria in gulf of mexico fish species. | recent developments in detection and enumeration of histamine-producing bacteria (hpb) have created powerful molecular-based tools to better understand the presence of spoilage bacteria and conditions, resulting in increased risk of scombrotoxin fish poisoning. we examined 235 scombrotoxin-forming fish from the gulf of mexico for the presence of high hpb. photobacterium damselae subsp. damselae was the most prevalent hpb (49%), followed by morganella morganii (14%), enterobacter aerogenes (4%), ... | 2015 | 26197285 |
| disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in enterobacter aerogenes. | hydrogen production by enterobacter aerogenes from glucose was enhanced by deleting the targeted ldha and adh genes responsible for two nadh-consuming pathways which consume most nadh generated from glycolysis. compared with the wild-type, the hydrogen yield of iam1183-δldha increased 1.5 fold. metabolic flux analysis showed both iam1183-δldha and iam1183-δadh exhibited significant changes in flux, including enhanced flux towards the hydrogen generation. the lactate production of iam1183-δldha s ... | 2015 | 26188552 |
| impact of an energy-conserving strategy on succinate production under weak acidic and anaerobic conditions in enterobacter aerogenes. | succinate is an important c4 building block chemical, and its production via fermentative processes in bacteria has many practical applications in the biotechnology field. one of the major goals of optimizing the bacterium-based succinate production process is to lower the culture ph from the current neutral conditions, as this would reduce total production costs. in our previous studies, we selected enterobacter aerogenes, a rapid glucose assimilator at ph 5.0, in order to construct a metabolic ... | 2015 | 26063229 |
| [emphysematous cystitis: report of one case]. | emphysematous cystitis is found in diabetic patients and in individuals with urinary stasis and immunosuppression. we report a 58-year-old male with hypertension, type 2 diabetes on insulin treatment and central nervous system vasculitis on immunosuppressive therapy. he was admitted with weight loss and gait instability. a pet-ct showed a circumscribed image of air in the bladder contour without involving the upper urinary tract, suggesting emphysematous cystitis. re-interrogated, the patient re ... | 2015 | 26005827 |
| biohydrogen and bioethanol production from biodiesel-based glycerol by enterobacter aerogenes in a continuous stir tank reactor. | crude glycerol from the biodiesel manufacturing process is being produced in increasing quantities due to the expanding number of biodiesel plants. it has been previously shown that, in batch mode, semi-anaerobic fermentation of crude glycerol by enterobacter aerogenes can produce biohydrogen and bioethanol simultaneously. the present study demonstrated the possible scaling-up of this process from small batches performed in small bottles to a 3.6-l continuous stir tank reactor (cstr). fresh feed ... | 2015 | 25970750 |
| ndm-1 encoded by a pndm-bj01-like plasmid p3sp-ndm in clinical enterobacter aerogenes. | a carbapenem-nonsusceptible enterobacter aerogenes strain named 3-sp was isolated from a human case of pneumonia in a chinese teaching hospital. ndm-1 carbapenemase is produced by a pndm-bj01-like conjugative plasmid designated p3sp-ndm to account for carbapenem resistance of 3-sp. p3sp-ndm was fully sequenced and compared with all publically available pndm-bj01-like plasmids. the genetic differences between p3sp-ndm and pndm-bj01 include only 18 single nucleotide polymorphisms, a 1 bp deletion ... | 2015 | 25926823 |
| phenolic compounds: strong inhibitors derived from lignocellulosic hydrolysate for 2,3-butanediol production by enterobacter aerogenes. | lignocellulosic biomass are attractive feedstocks for 2,3-butanediol production due to their abundant supply and low price. during the hydrolysis of lignocellulosic biomass, various byproducts are formed and their effects on 2,3-butanediol production were not sufficiently studied compared to ethanol production. therefore, the effects of compounds derived from lignocellulosic biomass (weak acids, furan derivatives and phenolics) on the cell growth, the 2,3-butanediol production and the enzymes ac ... | 2015 | 26479290 |
| engineering tobacco to remove mercury from polluted soil. | tobacco is an ideal plant for modification to remove mercury from soil. although several transgenic tobacco strains have been developed, they either release elemental mercury directly into the air or are only capable of accumulating small quantities of mercury. in this study, we constructed two transgenic tobacco lines: ntk-7 (a tobacco plant transformed with mert-merp-merb1-merb2-ppk) and ntp-36 (tobacco transformed with mert-merp-merb1-merb2-pcs1). the genes mert, merp, merb1, and merb2 were o ... | 2015 | 25690353 |
| comparison of microbiological and probiotic characteristics of lactobacilli isolates from dairy food products and animal rumen contents. | lactobacilli are employed in probiotic food preparations and as feed additives in poultry and livestock, due to health benefits associated with their consumption. the objective of this study was to evaluate and compare the probiotic potential of ten lactobacilli strains isolated from commercial dairy food products and animal rumen contents in new zealand. genetic identification of the isolates revealed that all belonged to the genus lactobacillus, specifically the species l. reuteri, l. rhamnosu ... | 2015 | 27682086 |
| polygalacturonase production by calcium alginate immobilized enterobacter aerogenes nbo2 cells. | bacterial cells of enterobacter aerogenes nbo2 were entrapped in calcium alginate beads in order to enhance polygalacturonase production compared to free cells. the optimized condition of 5 % (w/v) sodium alginate concentration, agitation speed of 250 rpm, and 15 beads of calcium alginate with inoculum size of 4 % (v/v; 5.4 × 10(7) cells/ml) produced 23.48 u/ml of polygalacturonase compared to free cells of 18.54 u/ml. there was about 26.6 % increment in polygalaturonase production. however, in ... | 2015 | 25547814 |
| draft genome sequence of enterobacter aerogenes, a dde-degrading and plant growth-promoting strain isolated from cucurbita pepo. | we report here the draft genome of enterobacter aerogenes, a gram-negative bacterium of the enterobacteriaceae isolated from cucurbita pepo root tissue. this bacterium shows 2,2-bis(p-chlorophenyl)-1,1-dichloroethylene (dde)-degrading potential and plant growth-promoting capacity. an analysis of its 4.5-mb draft genome will enhance the understanding of dde degradation pathways and phytoremediation applications for dde-contaminated soils. | 2015 | 25883299 |
| comparison of validated polymerase chain reaction and culture isolation for the routine detection of acanthamoeba from ocular samples. | acanthamoeba keratitis should be definitively diagnosed for appropriate therapy. our institution has validated polymerase chain reaction (pcr) as a routine diagnostic test to detect acanthamoeba dna from ocular samples. we compared pcr with culture isolation for detecting acanthamoeba from ocular samples. | 2015 | 25839345 |
| quantifying the effect of hand wash duration, soap use, ground beef debris, and drying methods on the removal of enterobacter aerogenes on hands. | hand washing is recognized as a crucial step in preventing foodborne disease transmission by mitigating crosscontamination among hands, surfaces, and foods. this research was undertaken to establish the importance of several keys factors (soap, soil, time, and drying method) in reducing microorganisms during hand washing. a nonpathogenic nalidixic acid-resistant enterobacter aerogenes surrogate for salmonella was used to assess the efficacy of using soap or no soap for 5 or 20 s on hands with or ... | 2015 | 25836392 |
| kpc-producing enterobacter aerogenes infection. | enterobacter is a common nosocomial microorganism and its carbapenem's resistance has increased. the management of these cases is unclear. | 2015 | 25722130 |
| combining urban wastewater treatment with biohydrogen production--an integrated microalgae-based approach. | the aim of the present work was the simultaneous treatment of urban wastewater using microalgae and the energetic valorization of the obtained biomass. chlorella vulgaris (cv), scenedesmus obliquus (sc) and a naturally occurring algal consortium c (consc) were grown in an urban wastewater. the nutrient removals were quite high and the treated water fits the legislation (pt dec-lei 236/98) in what concerns the parameters analysed (n, p, cod). after nutrient depletion the microalgae remained two m ... | 2015 | 25453433 |
| molecular characteristics of extended-spectrum cephalosporin-resistant enterobacteriaceae from humans in the community. | to investigate the molecular characteristics of extended-spectrum cephalosporin (esc)-resistant enterobacteriaceae collected during a cross-sectional study examining the prevalence and risk factors for faecal carriage of extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae in humans living in areas with high or low broiler density. | 2015 | 26029910 |
| frequency of bacterial agents isolated from patients with chronic sinusitis in northern iran. | sinusitis is a disease with significant health problems. diagnosis of sinusitis is clinical. the golden standard for detection of microorganisms that cause sinusitis is the culture of sinus drainage discharge. | 2015 | 26652093 |
| in vitro antibacterial and antibiotic-potentiation activities of the methanol extracts from beilschmiedia acuta, clausena anisata, newbouldia laevis and polyscias fulva against multidrug-resistant gram-negative bacteria. | the present study was designed to investigate the antibacterial activities of the methanol extracts from different parts of beilschmedia acuta kosterm (lauraceae), clausena anisata (willd) hook (rutaceae), newbouldia laevis seem (bignoniaceae) and polyscias fulva (hiern) harms (araliaceae) as well as their synergistic effects with antibiotics against a panel of gram-negative bacteria, including multi-drug resistant (mdr) phenotypes expressing active efflux pumps. | 2015 | 26590804 |
| deterioration to extinction of wastewater bacteria by non-thermal atmospheric pressure air plasma as assessed by 16s rdna-dgge fingerprinting. | the use of cold plasma jets for inactivation of a variety of microorganisms has recently been evaluated via culture-based methods. accordingly, elucidation of the role of cold plasma in decontamination would be inaccurate because most microbial populations within a system remain unexplored owing to the high amount of yet uncultured bacteria. the impact of cold atmospheric plasma on the bacterial community structure of wastewater from two different industries was investigated by metagenomic-based ... | 2015 | 26500637 |
| association of tellurite resistance with hypervirulent clonal groups of klebsiella pneumoniae. | tellurite-based selective growth media are used for several bacterial pathogens. we found that, in klebsiella pneumoniae, tellurite resistance is strongly associated with hypervirulent clonal group 23 (cg23), cg65, and cg86, providing a novel approach for screening environmental or carriage samples. the terw gene was also associated with these groups. | 2015 | 25631812 |
| novel glucose-1-phosphatase with high phytase activity and unusual metal ion activation from soil bacterium pantoea sp. strain 3.5.1. | phosphorus is an important macronutrient, but its availability in soil is limited. many soil microorganisms improve the bioavailability of phosphate by releasing it from various organic compounds, including phytate. to investigate the diversity of phytate-hydrolyzing bacteria in soil, we sampled soils of various ecological habitats, including forest, private homesteads, large agricultural complexes, and urban landscapes. bacterial isolate pantoea sp. strain 3.5.1 with the highest level of phytas ... | 2015 | 26209662 |
| development of molecular approach based on pcr assay for detection of histamine producing bacteria. | histamine fish poisoning becomes highly concern not only in public health but also economic aspect. histamine is produced from histidine in fish muscles by bacterial decarboxylase enzyme. several techniques have been developed to determine the level of histamine in fish and their products but the effective method for detecting histamine producing bacteria is still required. this study was attempted to detect histamine producing bacteria by newly developed pcr condition. histamine producing bacte ... | 2015 | 26787983 |
| clostridium perfringens epsilon toxin binds to membrane lipids and its cytotoxic action depends on sulfatide. | epsilon toxin (etx) is one of the major lethal toxins produced by clostridium perfringens types b and d, being the causal agent of fatal enterotoxemia in animals, mainly sheep and goats. etx is synthesized as a non-active prototoxin form (proetx) that becomes active upon proteolytic activation. etx exhibits a cytotoxic effect through the formation of a pore in the plasma membrane of selected cell targets where etx specifically binds due to the presence of specific receptors. however, the identit ... | 2015 | 26452234 |
| amym, a novel maltohexaose-forming α-amylase from corallococcus sp. strain egb. | a novel α-amylase, amym, was purified from the culture supernatant of corallococcus sp. strain egb. amym is a maltohexaose-forming exoamylase with an apparent molecular mass of 43 kda. based on the results of matrix-assisted laser desorption ionization-time of flight mass spectrometry and peptide mass fingerprinting of amym and by comparison to the genome sequence of corallococcus coralloides dsm 2259, the amym gene was identified and cloned into escherichia coli. amym encodes a secretory amylas ... | 2015 | 25576603 |
| transcriptional regulation, metal binding properties and structure of pden1597, an unusual zinc transport protein from paracoccus denitrificans. | atp-binding cassette (abc) transporters of the cluster 9 family are ubiquitous among bacteria and essential for acquiring zn(2+) and mn(2+) from the environment or, in the case of pathogens, from the host. these rely on a substrate-binding protein (sbp) to coordinate the relevant metal with high affinity and specificity and subsequently release it to a membrane permease for translocation into the cytoplasm. although a number of cluster 9 sbp structures have been determined, the structural attrib ... | 2015 | 25787075 |
| how fast-growing bacteria robustly tune their ribosome concentration to approximate growth-rate maximization. | maximization of growth rate is an important fitness strategy for bacteria. bacteria can achieve this by expressing proteins at optimal concentrations, such that resources are not wasted. this is exemplified for escherichia coli by the increase of its ribosomal protein-fraction with growth rate, which precisely matches the increased protein synthesis demand. these findings and others have led to the hypothesis that e. coli aims to maximize its growth rate in environments that support growth. howe ... | 2015 | 25754869 |
| common isolation of new delhi metallo-beta-lactamase 1-producing enterobacteriaceae in a large surgical hospital in vietnam. | this study sought to monitor the presence of carbapenem-resistant enterobacteriaceae (cre) and the proportion new delhi metallo-beta-lactamase 1 (ndm-1)-producing bacteria between august 2010 and december 2012 in a surgical hospital in vietnam. we identified 47 cre strains from a total of 4,096 enterobacteriaceae isolates (1.1 %) that were ndm-1-positive from 45 patients admitted to 11 different departments, with the majority being from the urology department. the ndm-1 gene was found in seven d ... | 2015 | 25732142 |
| further modification of the modified hodge test for detecting metallo-β-lactamase-producing carbapenem-resistant enterobacteriaceae. | the modified hodge test (mht) was designed to detect carbapenemase-producing enterobacteriaceae (cpe). this study evaluated variables to improve the performance of mht. | 2015 | 25932437 |
| in vivo evolution of bacterial resistance in two cases of enterobacter aerogenes infections during treatment with imipenem. | infections caused by multidrug resistant (mdr) bacteria are a major concern worldwide. changes in membrane permeability, including decreased influx and/or increased efflux of antibiotics, are known as key contributors of bacterial mdr. therefore, it is of critical importance to understand molecular mechanisms that link membrane permeability to mdr in order to design new antimicrobial strategies. in this work, we describe genotype-phenotype correlations in enterobacter aerogenes, a clinically pro ... | 2015 | 26398358 |
| the spread of bla oxa-48 and bla oxa-244 carbapenemase genes among klebsiella pneumoniae, proteus mirabilis and enterobacter spp. isolated in moscow, russia. | the spread of carbapenemase-producing enterobacteriaceae (cpe) is a great problem of healthcare worldwide. study of the spread for bla oxa-48-like genes coding epidemically significant carbapenemases among hospital pathogens is important for the regional and global epidemiology of antimicrobial resistance. | 2015 | 26526183 |
| first case of propionibacterium acnes urinary tract infection in a dog. | propionibacterium acnes has been rarely isolated as a commensal from dogs, but there is little evidence of pathogenicity. urinary tract infections are common in dogs and are typically caused by various commensal bacteria. here we present the first case report of a urinary tract infection caused by p. acnes. | 2015 | 26690238 |
| expression of lactate dehydrogenase in aspergillus niger for l-lactic acid production. | different engineered organisms have been used to produce l-lactate. poor yields of lactate at low ph and expensive downstream processing remain as bottlenecks. aspergillus niger is a prolific citrate producer and a remarkably acid tolerant fungus. neither a functional lactate dehydrogenase (ldh) from nor lactate production by a. niger is reported. its genome was also investigated for the presence of a functional ldh. the endogenous a. niger citrate synthase promoter relevant to a. niger acidogen ... | 2015 | 26683313 |
| pan-genomic analysis to redefine species and subspecies based on quantum discontinuous variation: the klebsiella paradigm. | various methods are currently used to define species and are based on the phylogenetic marker 16s ribosomal rna gene sequence, dna-dna hybridization and dna gc content. however, these are restricted genetic tools and showed significant limitations. | 2015 | 26420254 |