Publications

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transfer and expression of degradative and antibiotic resistance plasmids in acidophilic bacteria.the genetic accessibility of selected acidophilic bacteria was investigated to evaluate their applicability to degrading pollutants in acidic environments. the incp1 antibiotic resistance plasmids rp4 and pvk101 and the phenol degradation-encoding plasmid ppgh11 were transferred from neutrophilic bacteria into the extreme acidophilic eubacterium acidiphilium cryptum at frequencies of 1.8 x 10(-2) to 9.8 x 10(-4) transconjugants per recipient cell. the incq antibiotic resistance plasmid psup106 w ...19948161188
cultural and phylogenetic analysis of mixed microbial populations found in natural and commercial bioleaching environments.a range of autotrophic and heterotrophic enrichment cultures were established to determine the cultural bacterial diversity present in samples obtained from the acidic runoff of a chalcocite overburden heap and from laboratory-scale (1- to 4-liter) batch and continuous bioreactors which were being used for the commercial assessment of the bioleachability of zinc sulfide ore concentrates. strains identified as thiobacillus ferrooxidans, thiobacillus thiooxidans, "leptospirillum ferrooxidans," and ...19947517131
acpi, a novel isoschizomer of asuii from acidiphilium cryptum 25h, recognizes the sequence 5'tt decreases cgaa3'. 19911956800
glucose catabolism in strains of acidophilic, heterotrophic bacteria.pathways of glucose catabolism, potentially operational in six strains of obligately aerobic, acidophilic bacteria, including acidiphilium cryptum strain lhet2, were investigated by short-term radiorespirometry and enzyme assays. short-term radiorespirometry was conducted at ph 3.0 with specifically labeled [c]glucose. the high rate and yield of c-1 oxidized to co(2) indicated that the entner-doudoroff, pentose phosphate, or both pathways were operational in all strains. apparent nonequivalent y ...198516346876
phylogenetic analysis of the genera thiobacillus and thiomicrospira by 5s rrna sequences.5s rrna nucleotide sequences from thiobacillus neapolitanus, thiobacillus ferrooxidans, thiobacillus thiooxidans, thiobacillus intermedius, thiobacillus perometabolis, thiobacillus thioparus, thiobacillus versutus, thiobacillus novellus, thiobacillus acidophilus, thiomicrospira pelophila, thiomicrospira sp. strain l-12, and acidiphilium cryptum were determined. a phylogenetic tree, based upon comparison of these and other related 5s rrna sequences, is presented. the results place the thiobacilli ...19853924899
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