Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| chemical defence in a millipede: evaluation and characterization of antimicrobial activity of the defensive secretion from pachyiulus hungaricus (karsch, 1881) (diplopoda, julida, julidae). | the chemical defence of the millipede pachyiulus hungaricus is reported in the present paper, in which a chemical characterization is given and antimicrobial activity is determined. in total, independently of sex, 44 compounds were identified. all compounds belong to two groups: quinones and pentyl and hexyl esters of long-chain fatty acids. the relative abundances of quinones and non-quinones were 94.7% vs. 5.3% (males) and 87.3% vs. 12.7% (females), respectively. the two dominant quinones in b ... | 2016 | 27907048 |
| co-regulation of iron metabolism and virulence associated functions by iron and xibr, a novel iron binding transcription factor, in the plant pathogen xanthomonas. | abilities of bacterial pathogens to adapt to the iron limitation present in hosts is critical to their virulence. bacterial pathogens have evolved diverse strategies to coordinately regulate iron metabolism and virulence associated functions to maintain iron homeostasis in response to changing iron availability in the environment. in many bacteria the ferric uptake regulator (fur) functions as transcription factor that utilize ferrous form of iron as cofactor to regulate transcription of iron me ... | 2016 | 27902780 |
| the preparation and characterization of a novel sphingan wl from marine sphingomonas sp. wg. | sphingans, a group of structurally closely related bacterial exopolysaccharides produced by members of the genus sphingomonas, can be applied in a variety of industries such as food, cement, and personal care applications due to their high viscosity. a high sphingan-producing-bacterium, sphingomonas sp. wg can secret large quantity of sphingan designated as wl. to enhance the production of wl, a three-stage control strategy was applied and the highest wl production can reach 33.3 g/l. the rheolo ... | 2016 | 27883073 |
| genetic modulation of c-di-gmp turnover affects multiple virulence traits and bacterial virulence in rice pathogen dickeya zeae. | the frequent outbreaks of rice foot rot disease caused by dickeya zeae have become a significant concern in rice planting regions and countries, but the regulatory mechanisms that govern the virulence of this important pathogen remain vague. given that the second messenger cyclic di-gmp (c-di-gmp) is associated with modulation of various virulence-related traits in various microorganisms, here we set to investigate the role of the genes encoding c-di-gmp metabolism in the regulation of the bacte ... | 2016 | 27855163 |
| oxyr-regulated catalase catb promotes the virulence in rice via detoxifying hydrogen peroxide in xanthomonas oryzae pv. oryzae. | to facilitate infection, xanthomonas oryzae pv. oryzae (xoo), the bacterial blight pathogen of rice, needs to degrade hydrogen peroxide (h2o2) generated by the host defense response via a mechanism that is mediated by the transcriptional regulator oxyr. the catalase (cat) gene catb has previously been shown to belong to the oxyr regulon in xoo. however, its expression patterns and function in h2o2 detoxification and bacterial pathogenicity on rice remain to be elucidated. | 2016 | 27825304 |
| identification and classification of known and putative antimicrobial compounds produced by a wide variety of bacillales species. | gram-positive bacteria of the bacillales are important producers of antimicrobial compounds that might be utilized for medical, food or agricultural applications. thanks to the wide availability of whole genome sequence data and the development of specific genome mining tools, novel antimicrobial compounds, either ribosomally- or non-ribosomally produced, of various bacillales species can be predicted and classified. here, we provide a classification scheme of known and putative antimicrobial co ... | 2016 | 27821051 |
| old concepts, new molecules and current approaches applied to the bacterial nucleotide signalling field. | signalling nucleotides are key molecules that help bacteria to rapidly coordinate cellular pathways and adapt to changes in their environment. during the past 10 years, the nucleotide signalling field has seen much excitement, as several new signalling nucleotides have been discovered in both eukaryotic and bacterial cells. the fields have since advanced quickly, aided by the development of important tools such as the synthesis of modified nucleotides, which, combined with sensitive mass spectro ... | 2016 | 27672152 |
| marine fungi as producers of benzocoumarins, a new class of inhibitors of glycogen-synthase-kinase 3β. | the glycogen-synthase-kinase 3 (gsk-3) is an important target in drug discovery. this enzyme is involved in the signaling pathways of type 2 diabetes, neurological disorders, cancer, and other diseases. therefore, inhibitors of gsk-3 are promising drug candidates for the treatment of a broad range of diseases. here we report pannorin (1), alternariol (2), and alternariol-9-methylether (3) to be promising inhibitors of the isoform gsk-3β showing sub-μm ic50 values. the in vitro inhibition is in t ... | 2016 | 27801816 |
| draft genome sequence of the xanthan producer xanthomonas campestris lmg 8031. | here, we report the draft genome sequence of xanthomonas campestris lmg 8031, for which nearly no genetic information is available, despite its good xanthan-producing properties. we performed an illumina-based sequencing approach of lmg 8031. the genome revealed a 5.0-mb chromosome having 4,434 coding sequences and a g+c content of 65%. | 2016 | 27789631 |
| type three secretion system in attaching and effacing pathogens. | enteropathogenic escherichia coli and enterohemorrhagic e. coli are diarrheagenic bacterial human pathogens that cause severe gastroenteritis. these enteric pathotypes, together with the mouse pathogen citrobacter rodentium, belong to the family of attaching and effacing pathogens that form a distinctive histological lesion in the intestinal epithelium. the virulence of these bacteria depends on a type iii secretion system (t3ss), which mediates the translocation of effector proteins from the ba ... | 2016 | 27818950 |
| molecular basis for catalysis and substrate-mediated cellular stabilization of human tryptophan 2,3-dioxygenase. | tryptophan 2,3-dioxygenase (tdo) and indoleamine 2,3-dioxygenase (ido) play a central role in tryptophan metabolism and are involved in many cellular and disease processes. here we report the crystal structure of human tdo (htdo) in a ternary complex with the substrates l-trp and o2 and in a binary complex with the product n-formylkynurenine (nfk), defining for the first time the binding modes of both substrates and the product of this enzyme. the structure indicates that the dioxygenation react ... | 2016 | 27762317 |
| correction: nudix effectors: a common weapon in the arsenal of plant pathogens. | [this corrects the article doi: 10.1371/journal.ppat.1005704.]. | 2016 | 27737001 |
| potential for biocontrol of melanized fungi by actinobacteria isolated from intertidal region of ilha do mel, paraná, brazil. | actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16s region of ribosomal dna (rdna), which is conserved and present in all bacteria, is an important method of identification. melanized fungi are free-living organisms, which can also be pathogens of clinical importance. ... | 2016 | 27777013 |
| potential for biocontrol of melanized fungi by actinobacteria isolated from intertidal region of ilha do mel, paraná, brazil. | actinobacteria occur in many environments and have the capacity to produce secondary metabolites with antibiotic potential. identification and taxonomy of actinobacteria that produce antimicrobial substances is essential for the screening of new compounds, and sequencing of the 16s region of ribosomal dna (rdna), which is conserved and present in all bacteria, is an important method of identification. melanized fungi are free-living organisms, which can also be pathogens of clinical importance. ... | 2016 | 27777013 |
| dissection of exopolysaccharide biosynthesis in kozakia baliensis. | acetic acid bacteria (aab) are well known producers of commercially used exopolysaccharides, such as cellulose and levan. kozakia (k.) baliensis is a relatively new member of aab, which produces ultra-high molecular weight levan from sucrose. throughout cultivation of two k. baliensis strains (dsm 14400, nbrc 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources. this indica ... | 2016 | 27716345 |
| bioactivity of diterpens from the ethyl acetate extract of kingiodendron pinnatum rox. hams. | kingiodendron pinnatum rox. hams. is an endangered medicinal plant used in gonorrhoe, catarrhal conditions of genito-urinary and respiratory tracts. the scientific and pharmacological formulation of k. pinnatum has not been established so far though it is being traditionally used by tribes of the region. | 2016 | 27695270 |
| quantitative, image-based phenotyping methods provide insight into spatial and temporal dimensions of plant disease. | plant disease symptoms exhibit complex spatial and temporal patterns that are challenging to quantify. image-based phenotyping approaches enable multidimensional characterization of host-microbe interactions and are well suited to capture spatial and temporal data that are key to understanding disease progression. we applied image-based methods to investigate cassava bacterial blight, which is caused by the pathogen xanthomonas axonopodis pv. manihotis (xam). we generated xam strains in which in ... | 2016 | 27443602 |
| the arabidopsis cerk1-associated kinase pbl27 connects chitin perception to mapk activation. | perception of microbe-associated molecular patterns by host cell surface pattern recognition receptors (prrs) triggers the intracellular activation of mitogen-activated protein kinase (mapk) cascades. however, it is not known how prrs transmit immune signals to mapk cascades in plants. here, we identify a complete phospho-signaling transduction pathway from prr-mediated pathogen recognition to mapk activation in plants. we found that the receptor-like cytoplasmic kinase pbl27 connects the chitin ... | 2016 | 27679653 |
| multilocus variable number of tandem repeat analysis reveals multiple introductions in spain of xanthomonas arboricola pv. pruni, the causal agent of bacterial spot disease of stone fruits and almond. | xanthomonas arboricola pv. pruni is the causal agent of the bacterial spot disease of stone fruits, almond and some ornamental prunus species. in spain it was first detected in 2002 and since then, several outbreaks have occurred in different regions affecting mainly japanese plum, peach and almond, both in commercial orchards and nurseries. as the origin of the introduction(s) was unknown, we have assessed the genetic diversity of 239 x. arboricola pv. pruni strains collected from 11 spanish pr ... | 2016 | 27669415 |
| using deep learning for image-based plant disease detection. | crop diseases are a major threat to food security, but their rapid identification remains difficult in many parts of the world due to the lack of the necessary infrastructure. the combination of increasing global smartphone penetration and recent advances in computer vision made possible by deep learning has paved the way for smartphone-assisted disease diagnosis. using a public dataset of 54,306 images of diseased and healthy plant leaves collected under controlled conditions, we train a deep c ... | 2016 | 27713752 |
| structural differences in gut bacteria communities in developmental stages of natural populations of lutzomyia evansi from colombia's caribbean coast. | lutzomyia evansi, a phlebotomine insect endemic to colombia's caribbean coast, is considered to be the main vector of visceral and cutaneous leishmaniasis in the region. although insects of this species can harbor pathogenic and non-pathogenic microorganisms in their intestinal microbiota, there is little information available about the diversity of gut bacteria present in lutzomyia evansi. in this study, conventional microbiological methods and molecular tools were used to assess the compositio ... | 2016 | 27618991 |
| a self-inducible heterologous protein expression system in escherichia coli. | escherichia coli is an important experimental, medical and industrial cell factory for recombinant protein production. the inducible lac promoter is one of the most commonly used promoters for heterologous protein expression in e. coli. isopropyl-β-d-thiogalactoside (iptg) is currently the most efficient molecular inducer for regulating this promoter's transcriptional activity. however, limitations have been observed in large-scale and microplate production, including toxicity, cost and culture ... | 2016 | 27611846 |
| genomic characterization of the novel aeromonas hydrophila phage ahp1 suggests the derivation of a new subgroup from phikmv-like family. | aeromonas hydrophila is an opportunistic pathogenic bacterium causing diseases in human and fish. the emergence of multidrug-resistant a. hydrophila isolates has been increasing in recent years. in this study, we have isolated a novel virulent podophage of a. hydrophila, designated as ahp1, from waste water. ahp1 has a rapid adsorption (96% adsorbed in 2 min), a latent period of 15 min, and a burst size of 112 pfu per infected cell. at least eighteen ahp1 virion proteins were visualized in sds-p ... | 2016 | 27603936 |
| high-quality draft genome sequences of two xanthomonas pathotype strains infecting aroid plants. | we present here the draft genome sequences of bacterial pathogens of the araceae family, xanthomonas axonopodis pv. dieffenbachiae lmg 695 and xanthomonas campestris pv. syngonii lmg 9055, differing in host range. a comparison between genome sequences will help understand the mechanisms involved in tissue specificity and adaptation to host plants. | 2016 | 27587819 |
| exploiting indigenous knowledge of subsistence farmers' for the management and conservation of enset (ensete ventricosum (welw.) cheesman) (musaceae family) diversity on-farm. | enset (ensete ventricosum (welw.) cheesman) belongs to the order sctaminae, the family musaceae. the musaceae family is subdivided into the genera musa and ensete. enset is an important staple crop for about 20 million people in the country. recent publications on enset ethnobotany are insignificant when compared to the diverse ethnolingustic communities in the country. hence, this paper try to identify and document wealth of indigenous knowledge associated with the distribution, diversity, and ... | 2016 | 27586388 |
| regulation of organic hydroperoxide stress response by two ohrr homologs in pseudomonas aeruginosa. | pseudomonas aeruginosa ohrr and ospr are gene homologs encoding oxidant sensing transcription regulators. ospr is known to regulate gpx, encoding a glutathione peroxidase, while ohrr regulates the expression of ohr that encodes an organic peroxide specific peroxiredoxin. here, we show that ospr mediated gpx expression, like ohrr and ohr, specifically responds to organic hydroperoxides as compared to hydrogen peroxide and superoxide anion. furthermore, the regulation of these two systems is inter ... | 2016 | 27560944 |
| the intervening sequence of coxiella burnetii: characterization and evolution. | the intervening sequence (ivs) of coxiella burnetii, the agent of q fever, is a 428-nt selfish genetic element located in helix 45 of the precursor 23s rrna. the ivs element, in turn, contains an orf that encodes a hypothetical ribosomal s23 protein (s23p). although s23p can be synthesized in vitro in the presence of an engineered e. coli promoter and ribosome binding site, results suggest that the protein is not synthesized in vivo. in spite of a high degree of ivs conservation among different ... | 2016 | 27595093 |
| draft genome sequences of three xanthomonas translucens pathovar reference strains (pv. arrhenatheri, pv. poae and pv. phlei) with different specificities for forage grasses. | as causal agents of bacterial wilt in pastures and meadows, bacteria of the species xanthomonas translucens are a serious issue in forage grass production. so far, only little is known about host-pathogen interactions at the molecular level and the lack of comprehensive genome data impeded targeted breeding strategies towards resistant forage grass cultivars. here we announce the draft genome sequences of three grass-pathogenic xanthomonas translucens pathotype strains, i.e. pv. arrhenatheri lmg ... | 2016 | 27536340 |
| two distinct mechanisms of transcriptional regulation by the redox sensor yodb. | for bacteria, cysteine thiol groups in proteins are commonly used as thiol-based switches for redox sensing to activate specific detoxification pathways and restore the redox balance. among the known thiol-based regulatory systems, the marr/duf24 family regulators have been reported to sense and respond to reactive electrophilic species, including diamide, quinones, and aldehydes, with high specificity. here, we report that the prototypical regulator yodb of the marr/duf24 family from bacillus s ... | 2016 | 27531959 |
| interaction network and mass spectrometry data of xanthomonas citri subsp. citri surface proteins from differential proteomic analysis of infectious and non-infectious cells. | here we provide the mass-spectrometry and in silico interaction network dataset of proteins identified on our research article on surface proteomic analysis from xanthomonas citri subsp. citri (xac) cells grown in vivo (infectious) and in vitro (non-infectious, control) by 2d-dige approach. fluorescence labeling of proteins were performed on intact cells followed by cellular lysis and labeled spots from 2d gel differing in abundance between the two conditions (anova, p-value<0.05) were analyzed ... | 2016 | 27595129 |
| antimicrobial profile of arthrobacter kerguelensis vl-rk_09 isolated from mango orchards. | an actinobacterial strain vl-rk_09 having potential antimicrobial activities was isolated from a mango orchard in krishna district, andhra pradesh (india) and was identified as arthrobacter kerguelensis. the strain a. kerguelensis vl-rk_09 exhibited a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. production of bioactive metabolites by the strain was the highest in modified yeast extract malt extract dextrose broth, as compared to other media tested. lactose (1%) a ... | 2016 | 27515463 |
| dispersal of human and plant pathogens biofilms via nitric oxide donors at 4 °c. | recent studies suggest that nitric oxide donors capable of manipulating nitric oxide-mediated signaling in bacteria could induce dispersal of biofilms. encased in extracellular polymeric substances, human and plant pathogens within biofilms are significantly more resistant to sanitizers. this is particularly a problem in refrigerated environments where food is processed. in an exercise aimed to study the potential of nitric oxide donors as biofilm dispersal in refrigerated conditions, we compare ... | 2016 | 27457245 |
| from discovery to production: biotechnology of marine fungi for the production of new antibiotics. | filamentous fungi are well known for their capability of producing antibiotic natural products. recent studies have demonstrated the potential of antimicrobials with vast chemodiversity from marine fungi. development of such natural products into lead compounds requires sustainable supply. marine biotechnology can significantly contribute to the production of new antibiotics at various levels of the process chain including discovery, production, downstream processing, and lead development. howev ... | 2016 | 27455283 |
| the molecular basis for ubiquitin and ubiquitin-like specificities in bacterial effector proteases. | pathogenic bacteria rely on secreted effector proteins to manipulate host signaling pathways, often in creative ways. ce clan proteases, specific hydrolases for ubiquitin-like modifications (sumo and nedd8) in eukaryotes, reportedly serve as bacterial effector proteins with desumoylase, deubiquitinase, or, even, acetyltransferase activities. here, we characterize bacterial ce protease activities, revealing k63-linkage-specific deubiquitinases in human pathogens, such as salmonella, escherichia, ... | 2016 | 27425412 |
| promiscuous diffusible signal factor production and responsiveness of the xylella fastidiosa rpf system. | cell density-dependent regulation of gene expression in xylella fastidiosa that is crucial to its switching between plant hosts and insect vectors is dependent on rpff and its production of 2-enoic acids known as diffusible signal factor (dsf). we show that x. fastidiosa produces a particularly large variety of similar, relatively long-chain-length 2-enoic acids that are active in modulating gene expression. both x. fastidiosa itself and a pantoea agglomerans surrogate host harboring x. fastidio ... | 2016 | 27435463 |
| dcsbis (pa2771) from pseudomonas aeruginosa is a highly active diguanylate cyclase with unique activity regulation. | c-di-gmp (3',5' -cyclic diguanylic acid) is an important second messenger in bacteria that influences virulence, motility, biofilm formation, and cell division. the level of c-di-gmp in cells is controlled by diguanyl cyclases (dgcs) and phosphodiesterases (pdes). here, we report the biochemical functions and crystal structure of the potential diguanylase dcsbis (pa2771, a diguanylate cyclase with a self-blocked i-site) from pseudomonas aeruginosa pao1. the full-length dcsbis protein contains an ... | 2016 | 27388857 |
| dynamic light scattering of xanthan gum biopolymer in colloidal dispersion. | the dynamical properties of nanogels of xanthan gum (xg) with hydrodynamic radius controlled in a size range from 5 nm to 35 nm, were studied at the different xg concentrations in water/sodium bis-2-ethylhexyl-sulfosuccinate (aot)/decane reverse micelles (rms) vs. mass fraction of nano-droplet (mfd) at w = 40, using dynamic light scattering (dls). the diffusion study of nanometer-sized droplets by dls technique indicated that enhancing concentration of the xg polysaccharide resulted in exchangin ... | 2016 | 27489730 |
| next-generation sequencing diagnostics of bacteremia in septic patients. | bloodstream infections remain one of the major challenges in intensive care units, leading to sepsis or even septic shock in many cases. due to the lack of timely diagnostic approaches with sufficient sensitivity, mortality rates of sepsis are still unacceptably high. however a prompt diagnosis of the causative microorganism is critical to significantly improve outcome of bloodstream infections. although various targeted molecular tests for blood samples are available, time-consuming blood cultu ... | 2016 | 27368373 |
| discovery and identification of candidate genes from the chitinase gene family for verticillium dahliae resistance in cotton. | verticillium dahliae, a destructive and soil-borne fungal pathogen, causes massive losses in cotton yields. however, the resistance mechanism to v. dahilae in cotton is still poorly understood. accumulating evidence indicates that chitinases are crucial hydrolytic enzymes, which attack fungal pathogens by catalyzing the fungal cell wall degradation. as a large gene family, to date, the chitinase genes (chis) have not been systematically analyzed and effectively utilized in cotton. here, we ident ... | 2016 | 27354165 |
| morphological and physiological changes induced by contact-dependent interaction between candida albicans and fusobacterium nucleatum. | candida albicans and fusobacterium nucleatum are well-studied oral commensal microbes with pathogenic potential that are involved in various oral polymicrobial infectious diseases. recently, we demonstrated that f. nucleatum atcc 23726 coaggregates with c. albicans sn152, a process mainly mediated by fusobacterial membrane protein radd and candida cell wall protein flo9. the aim of this study was to investigate the potential biological impact of this inter-kingdom interaction. we found that f. n ... | 2016 | 27295972 |
| an innate immunity pathway in the moss physcomitrella patens. | map kinase (mpk) cascades in arabidopsis thaliana and other vascular plants are activated by developmental cues, abiotic stress, and pathogen infection. much less is known of mpk functions in nonvascular land plants such as the moss physcomitrella patens here, we provide evidence for a signaling pathway in p. patens required for immunity triggered by pathogen associated molecular patterns (pamps). this pathway induces rapid growth inhibition, a novel fluorescence burst, cell wall depositions, an ... | 2016 | 27268428 |
| identification of the c-terminal gh5 domain from cbcel9b/man5a as the first glycoside hydrolase with thermal activation property from a multimodular bifunctional enzyme. | caldicellulosiruptor bescii encodes at least six unique multimodular glycoside hydrolases crucial for plant cell wall polysaccharides degradation, with each having two catalytic domains separated by two to three carbohydrate binding modules. among the six enzymes, three have one n- or c-terminal gh5 domain with identical amino acid sequences. despite a few reports on some of these multimodular enzymes, little is known about how the conserved gh5 domains behave, which are believed to be important ... | 2016 | 27258548 |
| complete genome sequences of lytic bacteriophages of xanthomonas arboricola pv. juglandis. | three bacteriophages, f20-xaj, f29-xaj, and f30-xaj, with lytic activity against xanthomonas arboricola pv. juglandis were isolated from walnut trees (viii bío bío region, chile). these lytic bacteriophages have double-stranded dna (dsdna) genomes of 43,851 bp, 41,865 bp, and 44,262 bp, respectively. these are the first described bacteriophages with lytic activity against x. arboricola pv. juglandis that can be utilized as biocontrol agents. | 2016 | 27257210 |
| presence of extracellular dna during biofilm formation by xanthomonas citri subsp. citri strains with different host range. | xanthomonas citri subsp. citri (xcc) a strain causes citrus bacterial canker, a serious leaf, fruit and stem spotting disease of several citrus species. x. alfalfae subsp. citrumelonis (xac) is the cause of citrus bacterial spot, a minor disease of citrus nursery plants and x. campestris pv. campestris (xc) is a systemic pathogen that causes black rot of cabbage. xanthomonas spp. form biofilms in planta that facilitate the host infection process. herein, the role of extracellular dna (edna) was ... | 2016 | 27248687 |
| emerging insights on brazilian pepper tree (schinus terebinthifolius) invasion: the potential role of soil microorganisms. | invasive plant species constitute a major ecological and economic problem worldwide, often distorting trophic levels and ecosystem balance. numerous studies implicate factors ranging from environmental plasticity, competition for nutrient and space, and allelopathy in the success of invasive species in general. the brazilian pepper tree (bp) was introduced to the united states in the 1800s and has since become a category one invasive plant in florida. it has aggressively spread to about 3000 km( ... | 2016 | 27252726 |
| the influence of a modified lipopolysaccharide o-antigen on the biosynthesis of xanthan in xanthomonas campestris pv. campestris b100. | the exopolysaccharide xanthan is a natural product which is extensively used in industry. it is a thickening agent in many fields, from oil recovery to the food sector. xanthan is produced by the gram negative bacterium xanthomonas campestris pv. campestris (xcc). we analyzed the lipopolysaccharide (lps) of three mutant strains of the xcc wild type b100 to distinguish if the xanthan production can be increased when lps biosynthesis is affected. | 2016 | 27215401 |
| cloning and expression analysis of cinnamoyl-coa reductase (ccr) genes in sorghum. | cinnamoyl-coa reductase (ccr) is the first enzyme in the monolignol-specific branch of the lignin biosynthetic pathway. in this research, three sorghum ccr genes including sbccr1, sbccr2-1 and sbccr2-2 were cloned and characterized. analyses of the structure and phylogeny of the three ccr genes showed evolutionary conservation of the functional domains and divergence of function. transient expression assays in nicotiana benthamiana leaves demonstrated that the three ccr proteins were localized i ... | 2016 | 27231650 |
| detecting cell lysis using viscosity monitoring in e. coli fermentation to prevent product loss. | monitoring the physical or chemical properties of cell broths to infer cell status is often challenging due to the complex nature of the broth. key factors indicative of cell status include cell density, cell viability, product leakage, and dna release to the fermentation broth. the rapid and accurate prediction of cell status for hosts with intracellular protein products can minimise product loss due to leakage at the onset of cell lysis in fermentation. this article reports the rheological exa ... | 2016 | 27111912 |
| type ii toxin-antitoxin distribution and adaptive aspects on xanthomonas genomes: focus on xanthomonas citri. | prokaryotic toxin-antitoxin (ta) systems were first described as being designed to prevent plasmid loss in bacteria. however, with the increase in prokaryotic genome sequencing, recently many tas have been found in bacterial chromosomes, having other biological functions, such as environmental stress response. to date, only few studies have focused on ta systems in phytopathogens, and their possible impact on the bacterial fitness. this may be especially important for pathogens like xanthomonas ... | 2016 | 27242687 |
| plant genetic background increasing the efficiency and durability of major resistance genes to root-knot nematodes can be resolved into a few resistance qtls. | with the banning of most chemical nematicides, the control of root-knot nematodes (rkns) in vegetable crops is now based essentially on the deployment of single, major resistance genes (r-genes). however, these genes are rare and their efficacy is threatened by the capacity of rkns to adapt. in pepper, several dominant r-genes are effective against rkns, and their efficacy and durability have been shown to be greater in a partially resistant genetic background. however, the genetic determinants ... | 2016 | 27242835 |
| stenotrophomonas maltophilia phop, a two-component response regulator, involved in antimicrobial susceptibilities. | stenotrophomonas maltophilia, a gram-negative bacterium, has increasingly emerged as an important nosocomial pathogen. it is well-known for resistance to a variety of antimicrobial agents including cationic antimicrobial polypeptides (caps). resistance to polymyxin b, a kind of caps, is known to be controlled by the two-component system phopq. to unravel the role of phopq in polymyxin b resistance of s. maltophilia, a phop mutant was constructed. we found mics of polymyxin b, chloramphenicol, am ... | 2016 | 27159404 |
| supporting community annotation and user collaboration in the integrated microbial genomes (img) system. | the exponential growth of genomic data from next generation technologies renders traditional manual expert curation effort unsustainable. many genomic systems have included community annotation tools to address the problem. most of these systems adopted a "wiki-based" approach to take advantage of existing wiki technologies, but encountered obstacles in issues such as usability, authorship recognition, information reliability and incentive for community participation. | 2016 | 27118214 |
| genome-wide transcription start site mapping of bradyrhizobium japonicum grown free-living or in symbiosis - a rich resource to identify new transcripts, proteins and to study gene regulation. | differential rna-sequencing (drna-seq) is indispensable for determination of primary transcriptomes. however, using drna-seq data to map transcriptional start sites (tsss) and promoters genome-wide is a bioinformatics challenge. we performed drna-seq of bradyrhizobium japonicum usda 110, the nitrogen-fixing symbiont of soybean, and developed algorithms to map tsss and promoters. | 2016 | 27107716 |
| identification of novel major and minor qtls associated with xanthomonas oryzae pv. oryzae (african strains) resistance in rice (oryza sativa l.). | xanthomonas oryzae pv. oryzae (xoo) is the causal agent of bacterial leaf blight (bb), an emerging disease in rice in west-africa which can induce up to 50 % of yield losses. so far, no specific resistance gene or qtl to african xoo were mapped. the objectives of this study were to identify and map novels and specific resistance qtls to african xoo strains. | 2016 | 27107993 |
| edgar 2.0: an enhanced software platform for comparative gene content analyses. | the rapidly increasing availability of microbial genome sequences has led to a growing demand for bioinformatics software tools that support the functional analysis based on the comparison of closely related genomes. by utilizing comparative approaches on gene level it is possible to gain insights into the core genes which represent the set of shared features for a set of organisms under study. vice versa singleton genes can be identified to elucidate the specific properties of an individual gen ... | 2016 | 27098043 |
| polysaccharide-based membranes in food packaging applications. | plastic packaging is essential nowadays. however, the huge environmental problem caused by landfill disposal of non-biodegradable polymers in the end of life has to be minimized and preferentially eliminated. the solution may rely on the use of biopolymers, in particular polysaccharides. these macromolecules with film-forming properties are able to produce attracting biodegradable materials, possibly applicable in food packaging. despite all advantages of using polysaccharides obtained from diff ... | 2016 | 27089372 |
| differences in stability of seed-associated microbial assemblages in response to invasion by phytopathogenic microorganisms. | seeds are involved in the vertical transmission of microorganisms from one plant generation to another and consequently act as reservoirs for the plant microbiota. however, little is known about the structure of seed-associated microbial assemblages and the regulators of assemblage structure. in this work, we have assessed the response of seed-associated microbial assemblages of raphanus sativus to invading phytopathogenic agents, the bacterial strain xanthomonas campestris pv. campestris (xcc) ... | 2016 | 27077013 |
| systematic mutational analysis of histidine kinase genes in the nosocomial pathogen stenotrophomonas maltophilia identifies bfmak system control of biofilm development. | the gram-negative bacterium stenotrophomonas maltophilialives in diverse ecological niches. as a result of its formidable capabilities of forming biofilm and its resistance to multiple antibiotic agents, the bacterium is also a nosocomial pathogen of serious threat to the health of patients whose immune systems are suppressed or compromised. besides the histidine kinase rpfc, the two-component signal transduction system (tcs), which is the canonical regulatory machinery used by most bacterial pa ... | 2016 | 26873318 |
| 3-methylthiopropionic acid of rhizoctonia solani ag-3 and its role in the pathogenicity of the fungus. | studies were conducted to determine the role of 3-methylthioproprionic acid (mtpa) in the pathogenicity of potato stem canker, rhizoctonia solani, and the concentrations required to inhibit growth of r. solani under laboratory and plant house-based conditions. the experiments were laid out in a completely randomized design with five treatments and five replications. the treatments were 0, 1, 2, 4, and 8 mm concentrations of mtpa. the purified toxin exhibited maximal activity at ph 2.5 and 30°c. ... | 2016 | 27147928 |
| genetics and molecular mapping of black rot resistance locus xca1bc on chromosome b-7 in ethiopian mustard (brassica carinata a. braun). | black rot caused by xanthomonas campestris pv. campestris (pam.) dowson is the most destructive disease of cauliflower causing huge loss to the farmers throughout the world. since there are limited sources of resistance to black rot in b. oleracea (c genome brassica), exploration of a and b genomes of brassica was planned as these were thought to be potential reservoirs of black rot resistance gene(s). in our search for new gene(s) for black rot resistance, f2 mapping population was developed in ... | 2016 | 27023128 |
| natural personal care products-analysis of ingredient lists and legal situation. | many natural substances are classified as dangerous substances according to the european regulation on classification and labelling. are they used in natural personal care products today? one hundred ingredient lists were analyzed to find this out. | 2016 | 27752443 |
| the atp-dependent rna helicase hrpb plays an important role in motility and biofilm formation in xanthomonas citri subsp. citri. | rna helicases are enzymes that catalyze the separation of double-stranded rna (dsrna) using the free energy of atp binding and hydrolysis. dead/deah families participate in many different aspects of rna metabolism, including rna synthesis, rna folding, rna-rna interactions, rna localization and rna degradation. several important bacterial dead/deah-box rna helicases have been extensively studied. in this study, we characterize the atp-dependent rna helicase encoded by the hrpb (xac0293) gene usi ... | 2016 | 27005008 |
| crystal structure of the vicilin from solanum melongena reveals existence of different anionic ligands in structurally similar pockets. | crystal structure of a vicilin, sm80.1, was determined towards exploring its possible physiological functions. the protein was purified from solanum melongena by combination of ammonium sulphate fractionation and size exclusion chromatography. structure was determined ab initio at resolution of 1.5 å by x-ray crystallography showing the three-dimensional topology of the trimeric protein. each monomer of sm80.1 consists of two similar domains with hydrophobic binding pocket and each accommodating ... | 2016 | 27004988 |
| genome sequence of type strains of genus stenotrophomonas. | genomic resource of type strains and historically important strains of genus stenotrophomonas allowed us to reveal the existence of 18 distinct species by applying modern phylogenomic criterions. apart from stenotrophomonas maltophilia, s. africana represents another species of clinical importance. interestingly, pseudomonas hibsicola, p. beteli, and s. pavani that are of plant origin are closer to s. maltophilia than the majority of the environmental isolates. the genus has an open pan-genome. ... | 2016 | 27014232 |
| two overlapping two-component systems in xanthomonas oryzae pv. oryzae contribute to full fitness in rice by regulating virulence factors expression. | two-component signal transduction systems (tcss) are widely used by bacteria to adapt to the environment. in the present study, stos (stress tolerance-related oxygen sensor) and srekrs (salt response kinase, regulator, and sensor) were found to positively regulate extracellular polysaccharide (eps) production and swarming in the rice pathogen xanthomonas oryzae pv. oryzae (xoo). surprisingly, the absence of stos or srekrs did not attenuate virulence. to better understand the intrinsic functions ... | 2016 | 26957113 |
| crp is an activator of yersinia pestis biofilm formation that operates via a mechanism involving gmha and waaae-coad. | gmha encodes a phosphoheptose isomerase that catalyzes the biosynthesis of heptose, a conserved component of lipopolysaccharide (lps). gmha plays an important role in yersinia pestis biofilm blockage in the flea gut. waaa, waae, and coad constitute a three-gene operon waaae-coad in y. pestis. waaa encodes a transferase that is responsible for binding lipid-a to the core oligosaccharide of lps. waaa is a key determinant in y. pestis biofilm formation, and the waaa expression is positively regulat ... | 2016 | 27014218 |
| the placental membrane microbiome is altered among subjects with spontaneous preterm birth with and without chorioamnionitis. | preterm birth (ptb) is a leading cause of neonatal morbidity and mortality and is not uncommonly associated with chorioamnionitis. we recently have demonstrated that the placenta harbors a unique microbiome with similar flora to the oral community. we also have shown an association of these placental microbiota with ptb, history of antenatal infection, and excess maternal weight gain. on the basis of these previous observations, we hypothesized that the placental membranes would retain a microbi ... | 2016 | 26965447 |
| draft genome sequence of pantoea ananatis strain amg521, a rice plant growth-promoting bacterial endophyte isolated from the guadalquivir marshes in southern spain. | the rice endophyte pantoea ananatis amg521 shows several plant growth-promoting properties and promotes rice yield increases. its draft genome was estimated at 4,891,568 bp with 4,704 coding sequences (cds). the genome encodes genes for n-acylhomoserine lactone (ahl) synthases, ahl hydrolases, hyperadherence (yidq, yidp, and yidr), fusaric acid resistance, and oxidation of lignin, highlighting its biotechnological potential. | 2016 | 26893418 |
| structure of xanthan gum and cell ultrastructure at different times of alkali stress. | the effect of alkali stress on the yield, viscosity, gum structure, and cell ultrastructure of xanthan gum was evaluated at the end of fermentation process of xanthan production by xanthomonas campestris pv. manihotis 280-95. although greater xanthan production was observed after a 24h-alkali stress process, a lower viscosity was observed when compared to the alkali stress-free gum, regardless of the alkali stress time. however, this outcome is not conclusive as further studies on gum purificati ... | 2016 | 26887232 |
| in vitro and in vivo correlation of colon-targeted compression-coated tablets. | this study was performed to assess and correlate in vitro drug release with in vivo absorption of prednisolone (pdl) from a colon-targeted tablet prepared by compression coating of core tablet. in vivo drug absorption study was conducted using a high performance liquid chromatographic (hplc) method, which was developed and validated for the estimation of pdl in rabbit plasma. the calibration curve showed linearity in the concentration range of 0.05 to 50 μg/ml with the correlation coefficient (r ... | 2016 | 26989562 |
| draft genome sequences of two strains of xanthomonas arboricola pv. celebensis isolated from banana plants. | we report here the annotated draft genome sequences of strains xanthomonas arboricola pv. celebensis ncppb 1832 and ncppb 1630 (ncppb, national collection of plant pathogenic bacteria), both isolated from musa species in new zealand. this will allow the comparison of genomes between phylogenetically distant xanthomonads that have independently converged with the ability to colonize banana plants. | 2016 | 26868395 |
| characterization of the gntr family regulator hpar1 of the crucifer black rot pathogen xanthomonas campestris pathovar campestris. | the gntr family transcription regulator hpar1 identified from xanthomonas campestris pv. campestris has been previously shown to positively regulate the genes responsible for hypersensitive reaction and pathogenicity and to autorepress its own expression. here, we demonstrated that hpar1 is a global regulator that positively regulates diverse biological processes, including xanthan polysaccharide production, extracellular enzyme activity, cell motility and tolerance to various stresses. to inves ... | 2016 | 26818230 |
| natural products from marine fungi--still an underrepresented resource. | marine fungi represent a huge potential for new natural products and an increased number of new metabolites have become known over the past years, while much of the hidden potential still needs to be uncovered. representative examples of biodiversity studies of marine fungi and of natural products from a diverse selection of marine fungi from the author's lab are highlighting important aspects of this research. if one considers the huge phylogenetic diversity of marine fungi and their almost ubi ... | 2016 | 26784209 |
| identification of the key sequence in the flik c-terminal domain for substrate specificity switching in the flagellar protein secretion. | the flagellar hook is a short tubular structure located between the external filament and the membrane-bound basal body. the average hook length is 55 nm and is determined by the soluble protein flik and the integral membrane protein flhb. hook elongation is terminated by flik-mediated cessation of hook protein secretion, followed by the secretion of filamentous proteins. this process is referred to as the substrate specificity switch. switching of the secretion modes results from a direct inter ... | 2016 | 26527646 |
| the type ii secreted lipase/esterase lesa is a key virulence factor required for xylella fastidiosa pathogenesis in grapevines. | pierce's disease (pd) of grapevines is caused by xylella fastidiosa (xf), a xylem-limited gamma-proteobacterium that is responsible for several economically important crop diseases. the occlusion of xylem elements and interference with water transport by xf and its associated biofilm have been posited as the main cause of pd symptom development; however, xf virulence mechanisms have not been described. analysis of the xf secretome revealed a putative lipase/esterase (lesa) that was abundantly se ... | 2016 | 26753904 |
| isolation and characterization of pepper genes interacting with the cmv-p1 helicase domain. | cucumber mosaic virus (cmv) is a destructive pathogen affecting capsicum annuum (pepper) production. the pepper cmr1 gene confers resistance to most cmv strains, but is overcome by cmv-p1 in a process dependent on the cmv-p1 rna1 helicase domain (p1 helicase). here, to identify host factors involved in cmv-p1 infection in pepper, a yeast two-hybrid library derived from a c. annuum 'bukang' cdna library was screened, producing a total of 76 potential clones interacting with the p1 helicase. beta- ... | 2016 | 26751216 |
| effect of site-directed mutagenesis at the ggeef domain of the biofilm forming ggeef protein from vibrio cholerae. | vibrio cholerae, the cause of seven noted pandemics, leads a dual lifecycle-one in the human host in its virulent form, and the other as a sessile, non-virulent bacterium in aquatic bodies in surface biofilms. surface biofilms have been attributed to be associated with a ubiquitous protein domain present in all branches of bacteria, known as the ggd(/e)ef domain. while the diguanlyate cyclase activities of these proteins are universally established, the role of these proteins as diguanlyate-spec ... | 2016 | 26728467 |
| the dsf type quorum sensing signalling system rpff/r regulates diverse phenotypes in the opportunistic pathogen cronobacter. | several bacterial pathogens produce diffusible signal factor (dsf)-type quorum sensing (qs) signals to control biofilm formation and virulence. previous work showed that in burkholderia cenocepacia the rpffbc/rpfr system is involved in sensing and responding to dsf signals and that this signal/sensor gene pair is highly conserved in several bacterial species including cronobacter spp. here we show that c. turicensis lmg 23827(t) possesses a functional rpff/r system that is involved in the regula ... | 2016 | 26725701 |
| 7-hydroxycalamenene effects on secreted aspartic proteases activity and biofilm formation of candida spp. | the 7-hydroxycalamenenene-rich essential oil (eo) obtained from the leaves of croton cajucara (red morphotype) have been described as active against bacteria, protozoa, and fungi species. in this work, we aimed to evaluate the effectiveness of 7-hydroxycalamenenene against candida albicans and nonalbicans species. | 2016 | 27019560 |
| embryo localization enhances the survival of acidovorax citrulli in watermelon seeds. | acidovorax citrulli, the causal agent of bacterial fruit blotch (bfb) of cucurbits has been observed to survive for >34 years in stored melon and watermelon seeds. to better understand this remarkable longevity, we investigated the bacterium's tolerance to desiccation and the effect of bacterial localization in different watermelon seed tissues on its survival. we compared the ability of a. citrulli to tolerate desiccation on filter paper discs and on host (watermelon) and nonhost (cabbage, corn ... | 2016 | 26756827 |
| simultaneous detection of three bacterial seed-borne diseases in rice using multiplex polymerase chain reaction. | burkholderia glumae (bacterial grain rot), xanthomonas oryzae pv. oryzae (bacterial leaf blight), and acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. based on the 16s and 23s rdna sequences for a. avenae subsp. avenae and b. glumae, and transposase a gene sequence for x. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for b. glumae, 490 bp for x. oryzae, and 290 bp for a. avenae subsp. avenae with the 63°c as an optimum ... | 2016 | 27904465 |
| cloning, expression and characterization of a cold-adapted endo-1, 4-β-glucanase from citrobacter farmeri a1, a symbiotic bacterium of reticulitermes labralis. | many biotechnological and industrial applications can benefit from cold-adapted eglcs through increased efficiency of catalytic processes at low temperature. in our previous study, citrobacter farmeri a1 which was isolated from a wood-inhabiting termite reticulitermes labralis could secrete a cold-adapted eglc. however, its eglc was difficult to purify for enzymatic properties detection because of its low activity (0.8 u/ml). the objective of the present study was to clone and express the c. far ... | 2016 | 27843715 |
| trigger phosphodiesterases as a novel class of c-di-gmp effector proteins. | the bacterial second messenger c-di-gmp controls bacterial biofilm formation, motility, cell cycle progression, development and virulence. it is synthesized by diguanylate cyclases (with ggdef domains), degraded by specific phosphodiesterases (pdes, with eal of hd-gyp domains) and sensed by a wide variety of c-di-gmp-binding effectors that control diverse targets. c-di-gmp-binding effectors can be riboswitches as well as proteins with highly diverse structures and functions. the latter include ' ... | 2016 | 27672149 |
| pseudomonas fluorescens filamentous hemagglutinin, an iron-regulated protein, is an important virulence factor that modulates bacterial pathogenicity. | pseudomonas fluorescens is a common bacterial pathogen to a wide range of aquaculture animals including various species of fish. in this study, we employed proteomic analysis and identified filamentous hemagglutinin (fha) as an iron-responsive protein secreted by tss, a pathogenic p. fluorescens isolate. in vitro study showed that compared to the wild type, the fha mutant tssfha (i) exhibited a largely similar vegetative growth profile but significantly retarded in the ability of biofilm growth ... | 2016 | 27602029 |
| grape cultivar and sap culture conditions affect the development of xylella fastidiosa phenotypes associated with pierce's disease. | xylella fastidiosa is a xylem-limited bacterium in plant hosts and causes pierce's disease (pd) of grapevines, which differ in susceptibility according to the vitis species (spp.). in this work we compared x. fastidiosa biofilm formation and population dynamics when cultured in xylem saps from pd-susceptible and -resistant vitis spp. under different conditions. behaviors in a closed-culture system were compared to those in different sap-renewal cultures that would more closely mimic the physicoc ... | 2016 | 27508296 |
| the complete sequences and ecological roles of two incp-1β plasmids, phb44 and pbs64, isolated from the mycosphere of laccaria proxima. | two novel plasmids, coined phb44 and pbs64, were recently found in variovorax paradoxus strains hb44 and bs64 isolated from the mycosphere of laccaria proxima, on two different sampling occasions. we here describe the full sequences of phb44 and pbs64 and establish their evolutionary placement and ecological function. both plasmids, unique for mycospheric v. paradoxus, were around 58 kb in size. they possessed, in a very similar fashion, three main plasmid backbone regions, which were predicted ... | 2016 | 27445994 |
| pseudomonas syringae type iii effector hopaf1 suppresses plant immunity by targeting methionine recycling to block ethylene induction. | hopaf1 is a type iii effector protein of unknown function encoded in the genomes of several strains of pseudomonas syringae and other plant pathogens. structural modeling predicted that hopaf1 is closely related to deamidase proteins. deamidation is the irreversible substitution of an amide group with a carboxylate group. several bacterial virulence factors are deamidases that manipulate the activity of specific host protein substrates. we identified arabidopsis methylthioadenosine nucleosidase ... | 2016 | 27274076 |
| coordinated regulation of species-specific hydroxycinnamic acid degradation and siderophore biosynthesis pathways in agrobacterium fabrum. | the rhizosphere-inhabiting species agrobacterium fabrum (genomospecies g8 of the agrobacterium tumefaciens species complex) is known to degrade hydroxycinnamic acids (hcas), especially ferulic acid and p-coumaric acid, via the novel a. fabrum hca degradation pathway. gene expression profiles of a. fabrum strain c58 were investigated in the presence of hcas, using a c58 whole-genome oligoarray. both ferulic acid and p-coumaric acid caused variations in the expression of more than 10% of the c58 g ... | 2016 | 27060117 |
| biosynthesis of the acetyl-coa carboxylase-inhibiting antibiotic, andrimid in serratia is regulated by hfq and the lysr-type transcriptional regulator, admx. | infections due to multidrug-resistant bacteria represent a major global health challenge. to combat this problem, new antibiotics are urgently needed and some plant-associated bacteria are a promising source. the rhizobacterium serratia plymuthica a153 produces several bioactive secondary metabolites, including the anti-oomycete and antifungal haterumalide, oocydin a and the broad spectrum polyamine antibiotic, zeamine. in this study, we show that a153 produces a second broad spectrum antibiotic ... | 2016 | 26914969 |
| loss of gltb inhibits biofilm formation and biocontrol efficiency of bacillus subtilis bs916 by altering the production of γ-polyglutamate and three lipopeptides. | this study examined the contribution of gltb on biofilm formation and biocontrol efficiency of b. subtilis bs916. | 2016 | 27223617 |
| draft genome sequence of pseudomonas fluorescens strain et76, isolated from rice rhizosphere in northwestern morocco. | pseudomonas fluorescens et76 was isolated from rice rhizosphere in northwestern morocco. its draft genome was estimated to be 6,681,652 bp with 5,789 coding sequences (cdss). genes encoding for type i to vi secretion systems, pvdq, proteases, siderophores, hydrogen cyanide synthase, acc-deaminase, among others, highlight its potential use in biological control of plant pathogens. | 2016 | 27198014 |
| the draft genome sequence of the yersinia entomophaga entomopathogenic type strain mh96t. | here we report the draft genome of yersinia entomophaga type strain mh96t. the genome shows 93.8% nucleotide sequence identity to that of yersinia nurmii type strain apn3a-ct, and comprises a single chromosome of approximately 4,275,531 bp. in silico analysis identified that, in addition to the previously documented y. entomophaga yen-tc gene cluster, the genome encodes a diverse array of toxins, including two type iii secretion systems, and five rhs-associated gene clusters. as well as these mu ... | 2016 | 27187466 |
| biofilms and cyclic di-gmp (c-di-gmp) signaling: lessons from pseudomonas aeruginosa and other bacteria. | the cyclic di-gmp (c-di-gmp) second messenger represents a signaling system that regulates many bacterial behaviors and is of key importance for driving the lifestyle switch between motile loner cells and biofilm formers. this review provides an up-to-date compendium of c-di-gmp pathways connected to biofilm formation, biofilm-associated motilities, and other functionalities in the ubiquitous and opportunistic human pathogen pseudomonas aeruginosa this bacterium is frequently adopted as a model ... | 2016 | 27129226 |
| characterization of novel bacteriophages for biocontrol of bacterial blight in leek caused by pseudomonas syringae pv. porri. | pseudomonas syringae pv. porri, the causative agent of bacterial blight in leek (allium porrum), is increasingly frequent causing problems in leek cultivation. because of the current lack of control measures, novel bacteriophages were isolated to control this pathogen using phage therapy. five novel phages were isolated from infected fields in flanders (vb_psym_kil1, vb_psym_kil2, vb_psym_kil3, vb_psym_kil4, and vb_psym_kil5), and were complemented with one selected host range mutant phage (vb_p ... | 2016 | 27014204 |
| bioactive compounds produced by strains of penicillium and talaromyces of marine origin. | in recent years, the search for novel natural compounds with bioactive properties has received a remarkable boost in view of their possible pharmaceutical exploitation. in this respect the sea is entitled to hold a prominent place, considering the potential of the manifold animals and plants interacting in this ecological context, which becomes even greater when their associated microbes are considered for bioprospecting. this is the case particularly of fungi, which have only recently started t ... | 2016 | 26901206 |
| the diguanylate cyclase hsbd intersects with the hptb regulatory cascade to control pseudomonas aeruginosa biofilm and motility. | the molecular basis of second messenger signaling relies on an array of proteins that synthesize, degrade or bind the molecule to produce coherent functional outputs. cyclic di-gmp (c-di-gmp) has emerged as a eubacterial nucleotide second messenger regulating a plethora of key behaviors, like the transition from planktonic cells to biofilm communities. the striking multiplicity of c-di-gmp control modules and regulated cellular functions raised the question of signaling specificity. are c-di-gmp ... | 2016 | 27792789 |
| editorial: harnessing useful rhizosphere microorganisms for pathogen and pest biocontrol. | 2016 | 27807429 | |
| salicylic acid is involved in the basal resistance of tomato plants to citrus exocortis viroid and tomato spotted wilt virus. | tomato plants expressing the nahg transgene, which prevents accumulation of endogenous salicylic acid (sa), were used to study the importance of the sa signalling pathway in basal defence against citrus exocortis viroid (cevd) or tomato spotted wilt virus (tswv). the lack of sa accumulation in the cevd- or tswv-infected nahg tomato plants led to an early and dramatic disease phenotype, as compared to that observed in the corresponding parental money maker. addition of acibenzolar-s-methyl, a ben ... | 2016 | 27893781 |
| inhibition of xanthomonas fragariae, causative agent of angular leaf spot of strawberry, through iron deprivation. | in commercial production settings, few options exist to prevent or treat angular leaf spot (als) of strawberry, a disease of economic importance and caused by the bacterial pathogen xanthomonas fragariae. in the process of isolating and identifying x. fragariae bacteria from symptomatic plants, we observed growth inhibition of x. fragariae by bacterial isolates from the same leaf macerates. identified as species of pseudomonas and rhizobium, these isolates were confirmed to suppress growth of x. ... | 2016 | 27790193 |
| two distinct cardiolipin synthases operate in agrobacterium tumefaciens. | cardiolipin (cl) is a universal component of energy generating membranes. in most bacteria, it is synthesized via the condensation of two molecules phosphatidylglycerol (pg) by phospholipase d-type cardiolipin synthases (pld-type cls). in the plant pathogen and natural genetic engineer agrobacterium tumefaciens cl comprises up to 15% of all phospholipids in late stationary growth phase. a. tumefaciens harbors two genes, atu1630 (cls1) and atu2486 (cls2), coding for pld-type cls. heterologous exp ... | 2016 | 27472399 |