Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| coxsackievirus b5 and the relationship to swine vesicular disease virus. | 1997 | 9294928 | |
| likelihood of introducing selected exotic diseases to domestic swine in the continental united states of america through uncooked swill. | to help policy makers determine the need for current regulations (which require cooking of swill prior to feeding to swine), an assessment of the likelihood of exposing domestic swine in the continental united states of america (usa) to selected foreign animal disease agents by feeding uncooked swill was carried out. the hazard was assumed to originate from contraband food items entering the usa and subsequently being discarded in household waste. such food waste may be collected by licensed was ... | 1997 | 9329117 |
| detection of early infection of swine vesicular disease virus in porcine cells and skin sections. a comparison of immunohistochemistry and in-situ hybridization. | sensitive methods are required to study the early pathogenesis of swine vesicular diseases (svd). therefore, two new methods, immunohistochemistry (ihc) and in-situ hybridization (ish), were developed and tested for their specificity and sensitivity. with these methods the svd virus (svdv) infection in cytospins of primary porcine kidney cells and in frozen skin sections was investigated. both ihc and the ish showed a specific cytoplasmic staining, but the ihc detected more infected cells than t ... | 1997 | 9389406 |
| rapid and sensitive polymerase chain reaction based detection and typing of foot-and-mouth disease virus in clinical samples and cell culture isolates, combined with a simultaneous differentiation with other genomically and/or symptomatically related viruses. | reverse transcription followed by the polymerase chain reaction method (pcr) allowed the detection of foot-and-mouth disease virus (fmdv), regardless of the serotype. a primer set corresponding to highly conserved regions of the 2b sequence was selected. by combining in a single reaction tube specific primer pairs for fmdv, swine vesicular disease virus, (svdv), encephalomyocarditis virus (emcv) and bovine viral diarrhea virus (bvdv), all four viruses could be identified and differentiated in on ... | 1996 | 8634024 |
| genetic and phylogenetic clustering of enteroviruses. | genetic and phylogenetic analysis of enteroviruses showed that in the 5'ncr enteroviruses formed three clusters: polioviruses (pvs), coxsackievirus a type 21 (cav21), cav24 and enterovirus type 70 (env70) formed one cluster; coxsackievirus b isolates (cbvs), cav9, cav16, env71, echovirus type 11 (ev11), ev12 and all partially sequenced echoviruses and swine vesicular disease virus (svdv) belonged to another cluster and bovine enteroviruses (bevs) formed the third cluster. in the capsid coding re ... | 1996 | 8760417 |
| identification of the location of antigenic sites of swine vesicular disease virus with neutralization-resistant mutants. | neutralization sites on swine vesicular disease virus (svdv) have been identified by sequence analysis of neutralization-resistant mutants. eight neutralizing monoclonal antibodies (mabs) were produced and neutralization-resistant mutants were selected with the mabs. resistance of the mutants to neutralization was shown using the stab-neutralization method, and the results indicated the presence of five neutralization sites on the virus. the location of each site was identified from amino acid c ... | 1995 | 8847515 |
| differential diagnosis and genetic analysis of the antigenically related swine vesicular disease virus and coxsackie viruses. | monoclonal antibodies directed against an isolate of swine vesicular disease virus (svdv), characterized by virus neutralization tests and competition assays, were used to compare svdv isolates and isolates of the antigenically related coxsackie viruses by elisa. svdv-specific reaction patterns and one specific for coxsackie viruses were observed. this provided a method for distinguishing between these enteroviruses. in addition, rt-pcrs were undertaken with coxsackie virus and svdv genomes. dif ... | 1995 | 7673387 |
| development of two novel monoclonal antibody-based elisas for the detection of antibodies and the identification of swine isotypes against swine vesicular disease virus. | two novel formats of elisa for the detection of antibodies against swine vesicular disease (svd) virus were developed. one of the tests described is a monoclonal antibody-based competitive elisa (mac-elisa). in this test, specific antibodies in serum are detected due to their ability to compete with a neutralizing monoclonal antibody (mab). the second is an indirect trapping elisa which employs isotype-specific mabs to detect swine igg or igm specific for svd virus. the diagnostic sensitivity an ... | 1995 | 7769029 |
| genotypic variation in coxsackievirus b5 isolates from three different outbreaks in the united states. | genomic sequences in vp1/2a and 5'-non-coding region of 10 isolates of coxsackievirus b5 from three outbreaks were compared with published sequences of another coxsackievirus b5, swine vesicular disease virus, coxsackievirus b1, coxsackievirus b3, and coxsackievirus b4. isolates of coxsackievirus b5 from the same outbreak showed close relations, not exceeding 7.2% in nucleotide differences. differences were greater between isolates from different outbreaks, varying between 8.4 and 16%. we have a ... | 1995 | 8578854 |
| intratypic genome variability of the coxsackievirus b1 2a protease region. | to analyse the intratypic genome variability of coxsackievirus b1, 17 coxsackievirus b1 isolates were collected over a period of 10 years. nucleotide sequences of the 2a coding region of the various coxsackievirus b1 isolates and known sequences of other enteroviruses were compared. the maximum diversity observed within the entire group of coxsackievirus b1 isolates was 25%. comparison of deduced amino acid sequences revealed a maximum diversity of 5%. phylogenetic analysis demonstrates a close ... | 1994 | 8126468 |
| the complete nucleotide sequence of a pathogenic swine vesicular disease virus isolated in japan (j1'73) and phylogenetic analysis. | 1993 | 8367308 | |
| complete nucleotide sequence of a coxsackie b5 virus and its relationship to swine vesicular disease virus. | we report the first complete nucleotide sequence of the picornavirus coxsackievirus b5 (cb5), strain 1954/uk/85, an isolate from a case of hand-foot-and-mouth disease. we have compared the sequence with those of other coxsackie b viruses, coxsackievirus a9, poliovirus and swine vesicular disease virus (svdv). the genes encoding the three major capsid proteins are most closely related to those of svdv but the 5' and 3' noncoding regions and the p3 gene are more similar to the corresponding region ... | 1993 | 8388019 |
| an indirect sandwich elisa for the identification of bovine enteroviruses. | an indirect sandwich elisa is described for the detection of bovine enteroviruses. the assay was developed as an alternative to the complement fixation test and proved to be more sensitive and convenient. ten bovine enterovirus prototype strains were easily discriminated. no cross-reactions were observed with other picornaviruses including foot-and-mouth disease viruses, swine vesicular disease virus, porcine enteroviruses and bovine rhinovirus. | 1993 | 8388399 |
| detection of foot-and-mouth disease virus rna in clinical samples and cell culture isolates by amplification of the capsid coding region. | foot-and-mouth disease is one of the most economically important virus diseases of livestock. two important requirements for the control of this disease are rapid laboratory diagnosis and epidemiological investigation. the use of the polymerase chain reaction method (pcr) to amplify specific nucleic acid regions offers the unique possibility of combining swift viral detection with the production of genetic material suitable for sequencing and other methods of molecular epidemiological analysis. ... | 1993 | 8391540 |
| characterization of a bicistronic retroviral vector composed of the swine vesicular disease virus internal ribosome entry site. | we cloned the 5' nontranslated region (ntr) from the genome of swine vesicular disease virus (svdv), a member of the family picornaviridae, and used it to construct a bicistronic retroviral vector. the vector is characterized by coexpression of two genes from a single transcript. we found that inclusion of the 5' ntr of svdv did not negate the viral vector titer. protein analysis indicated that the 5' ntr could efficiently direct internal initiation, thus allowing the downstream gene to be trans ... | 1993 | 8445723 |
| no serological evidence for the presence of swine vesicular disease virus in south africa. | an indirect elisa incorporating a protein a-peroxidase conjugate was developed for detecting antibodies to swine vesicular disease virus (svdv) in pig sera. this test and a conventional virus neutralization test were found to be equally sensitive. a total of 2846 pig sera collected from various abattoirs in south africa were tested using the indirect elisa. no serological evidence of infection with svdv in pigs in south africa was found. | 1992 | 1437026 |
| production of infectious swine vesicular disease virus from cloned cdna in mammalian cells. | full-length cdna clones of the swine vesicular disease virus (svdv) were constructed from subgenomic cdna clones in the expression vector psvl (psvls00). the direct transfection of mammalian cells with plasmid psvls00 results in the production of infectious virus. the recovered virus was neutralized completely by anti-svdv guinea-pig serum, but did show a difference in plaque morphology from the parental virus. | 1990 | 2167939 |
| the complete nucleotide sequence of a pathogenic swine vesicular disease virus. | the nucleotide sequence of a swine vesicular disease virus (svdv) strain that is pathogenic for pigs has been determined and compared with that of a non-pathogenic strain of svdv, as well as a number of other enteroviruses. it shows only 98 base changes in comparison with a non-pathogenic strain of svdv (inoue et al., 1989, j. gen. virol. 70, 919-934). fourteen of these nucleotide differences between the pathogenic and the non-pathogenic svdv strains occur in the 5' non-coding region which, by a ... | 1990 | 2168111 |
| the complete nucleotide sequence of swine vesicular disease virus. | the complete nucleotide sequence of the genome of the enterovirus swine vesicular disease virus (svdv; h/3 '76) isolated from a healthy pig has been determined using molecular cloning and dna sequencing techniques. the rna genome was 7400 nucleotides long, excluding the poly(a) tract, and appeared to encode a single polyprotein of 2185 amino acids. the predicted amino acid sequence of the polyprotein showed close homology (around 90%) to that of the previously sequenced coxsackieviruses b1, b3 a ... | 1989 | 2543767 |
| an enzyme-linked immunosorbent assay (elisa) for the detection and quantification of antibodies against swine vesicular disease virus (svdv). | a liquid phase blocking sandwich elisa has been compared with virus neutralisation for testing pig sera for antibodies against swine vesicular disease (svd) virus. highest infectivity titre of svd virus was obtained using a multiplicity of infection of 30 pfu/cell and harvesting after 21 h. titres obtained for 300 clinically normal animals were assessed by elisa and 89% were found to be 1/6 or less. results were skewed and spread up to 1/45. comparison of known positive sera resulted in a correl ... | 1989 | 2778030 |
| [the reproduction of swine vesicular disease virus in microcells and microcytoplasts]. | 1988 | 2849265 | |
| induction of neutralizing antibodies by structural proteins vp1 and vp2 of swine vesicular disease virus. | 1987 | 3033376 | |
| induction and characterization of swine beta interferon. | newcastle disease virus (ndv) strain "h" and polyinosinic-polycytidylic acid (poly i:c) were used for interferon (ifn) induction in secondary pig kidney cells. a functional ifn system was detected and characterized. a wide similarity with the correspondent human and bovine systems was appreciated, with particular regard to the kinetics of synthesis. a glycosylated protein was essential for activity in bovine cells, but not in swine cells. poly i:c proved to be a very weak inducer, even in condit ... | 1987 | 3034503 |
| antigenic comparison of the polypeptides of foot-and-mouth disease virus serotypes and other picornaviruses. | the cross-reactivity of proteins coded for by the seven serotypes of foot-and-mouth disease virus (fmdv) was assessed by reaction of infected cell lysates with polyclonal and monospecific antisera against the structural and nonstructural proteins of fmdv type a12 strain 119ab. it was shown that the homologous polypeptides from most serotypes are antigenically related. the least cross-reactivity occurred between vp1, vp3, and the protease (3c) of type a12 and south african territories types 1 and ... | 1987 | 2437694 |
| embryo transfer as a means of controlling the transmission of viral infections. ix. the in vitro exposure of zona pellucida-intact porcine embryos to swine vesicular disease virus. | when zona pellucida-intact porcine embryos were exposed to 10(7) plaque-forming units (pfu)/ml of swine vesicular disease virus (svdv) and then washed, infectious virus could be isolated from all of the embryos. culturing the embryos for 24 or 48 h or treating the embryos with pronase, trypsin, or antiserum after virus exposure and washing reduced the number of embryos carrying virus and lessened the amount of virus on each of the embryos. none of the treatments, however, was capable of disinfec ... | 1987 | 16726249 |
| embryo transfer as a means of controlling the transmission of viral infections. x. the in vivo exposure of zona pellucida-intact porcine embryos to swine vesicular disease virus. | two experiments involving the transfer of embryos from donors infected with swine vesicular disease virus (svdv) to "clean" recipients were carried out. in experiment 1, 47 embryos were collected from 4 svdv-infected donors and transferred to 2 recipients that subsequently produced 10 piglets. all of the recipients and piglets remained seronegative for svdv. in addition to the transfers, 10 embryos and 58 unfertilized eggs from the infected donors were assayed in vitro and found to be negative f ... | 1987 | 16726250 |
| replicase gene of coxsackievirus b3. | a cdna copy covering two-thirds of the coxsackievirus b3 genome was cloned in the psti site of the pbr322 vector. a nucleotide sequence containing the gene for the viral replicase and the 3' noncoding region of the coxsackievirus b3 genome was determined. the predicted amino acid sequence of the coxsackievirus b3 replicase was shown to be remarkably similar to that of the poliovirus 1 replicase. the 3' noncoding region, in contrast, was only weakly homologous to the poliovirus 1 sequence but sho ... | 1984 | 6088796 |
| plaque morphology and pathogenicity for newborn mice of swine vesicular disease virus. i. wild strains and their clones. | the population of wild swine vesicular disease virus (svdv) strains was found non-homogeneous as manifested by varying plaque size and different pathogenicity of the clones obtained. the clones derived from large plaques (5-9 mm)--dominating among wild strains--were more virulent for newborn mice than those obtained from smaller plaques (1-2 mm). to evaluate the pathogenicity of wild svdv strains the dose index was calculated; the clones were compared by dose index and theoretical pathogenicity ... | 1983 | 6138982 |
| plaque morphology and pathogenicity for newborn mice of swine vesicular disease virus. ii. temperature-dependent mutants and their clones. | from wild swine vesicular disease virus (svdv) strains temperature-dependent (td) mutants td 27 degrees c, td 32 degrees c and td 42 degrees c were derived. differences were noted in their pathogenicity for newborn mice. the non-homogeneity of the td populations was manifested by formation of plaques of various sizes and confirmed by differential pathogenicity of the clones derived from them. a higher pathogenicity of the td mutants and their clones was associated not only with larger plaques, b ... | 1983 | 6138983 |
| the propagation of a strain of swine vesicular disease virus in one-day-old mice. | 1983 | 6323803 | |
| comparative studies of united kingdom isolates of swine vesicular disease virus. | the characteristics of four united kingdom isolates of swine vesicular disease (svd) virus from 1981 to 1982 have been compared with those of an isolate obtained from the first outbreak of swine vesicular disease diagnosed in the united kingdom in 1972. when the virus structural proteins were examined by polyacrylamide gel electrophoresis the four isolates from 1981-82 all had the same polypeptide pattern, which was different from that of the 1972 isolate. immunodiffusion tests with the 1972 iso ... | 1983 | 6364280 |
| inactivation by gamma irradiation of animal viruses in simulated laboratory effluent. | several animal viruses were treated with gamma radiation from a 60co source under conditions which might be found in effluent from an animal disease laboratory. swine vesicular disease virus, vesicular stomatitis virus, and blue-tongue virus were irradiated in tissues from experimentally infected animals. pseudorabies virus, fowl plague virus, swine vesicular disease virus, and vesicular stomatitis virus were irradiated in liquid animal feces. all were tested in animals and in vitro. the d10 val ... | 1982 | 6285822 |
| blood dried on filter or blotting paper for the detection of antibody against swine vesicular disease virus by enzyme-linked immunosorbent assay. | 1982 | 6294958 | |
| the production and use of swine vesicular disease virus from pig kidney monolayer cells grown on the surface of 3-mm-diameter glass spheres. | 1982 | 18546115 | |
| electron microscopy of cells cultured in serum-free medium after inoculation of swine vesicular disease virus. | morphological alterations of ib-rs-2 cells cultured in serum-free maintenance medium after inoculation of swine vesicular disease virus (svdv) were studied electron-microscopically. cells harvested 0 to 3 hours after inoculation showed no alterations. cellular alterations were observed from 4 to 7 hours after inoculation. many vacuoles appeared just beneath the cytoplasmic membrane and were separated by thin cytoplasm. narrow pathways were sometimes seen in the degenerative cells. they occasiona ... | 1981 | 7335124 |
| comparison of proliferation and cytopathogenicity of swine vesicular virus and coxsackievirus b5. | sequential appearance of both swine vesicular disease virus and coxsackievirus b5 antigens in a pig kidney cell line was studied by immunofluorescence and electron microscopy. the replication cycle of each virus was approximately 3-4 h. viral antigens were demonstrable in the cytoplasm 2 h after inoculation. a compact mass of fluorescence was seen when cells showed cytopathogenic effect at 5.5 h. after 3 h, a few viral particles, seen by electron microscopy, were in the cytoplasm. morphological ... | 1981 | 6269809 |
| a comparative investigation of antibody to swine vesicular disease virus using counter immunoelectrophoresis, serum neutralization and double immunodiffusion tests. | 1980 | 6255777 | |
| crystal formation of swine vesicular disease virus in porcine kidney cells (ib-rs-2 cells). | crystal formation of swine vesicular disease virus (svdv) in ib-rs-2 cells was studied by electron microscopy. cells were harvested 0, 3, 3.5, 4, 4.5, 5, 6 and 7 hours after inoculation. crystalline arrays of svdv was first observed in the cytoplasm of a few cells 4.5 hours after inoculation. in the cytoplasm of many cells harvested at 5 hours, 1 to 3 crystalline arrays of svdv were observed. after that, a small number of cells had crystalline arrays in the cytoplasm. the cells with crystalline ... | 1980 | 6267485 |
| enzyme linked immunoassay and fluorescent antibody techniques in the diagnosis of viral diseases using staphylococcal protein-a instead of anti-gamma-globulins. | staphylococcal protein-a (spa) is known to interact with the crystallizable fragment (fc) of igg molecules from several species. in the present study, spa coupled to either fluorescein isothiocyanate (fitc) or peroxidase was used in place of antisera to igg for the fluorescent antibody (fa) techniques and the enzyme linked immunoassay (elisa). the spa conjugates produced low background staining when applied in these techniques, and provide a rapid, highly specific and sensitive means for the ide ... | 1980 | 15612263 |
| in vivo and in vitro studies on temperature-sensitive mutants of swine vesicular disease virus. | two temperature-sensitive mutants of the ukg 27/72 strain of swine vesicular disease virus were isolated in tissue culture and a third was derived following adaptation in mice. all three were found to have similar growth restrictive temperatures, but varied considerably in their virulence when administered to pigs. the route of inoculation appeared to exert a considerable influence on the apparent degree of attenuation, the antibody titre engendered and the transmission of disease to pigs held i ... | 1979 | 226626 |
| [diptera of calliphora genus, potential carriers and spreaders of swine vesicular disease virus]. | swine vesicular disease virus ingested by the adult fly calliphora persists several days in the digestive tract of the insect and is eliminated in feces. the virus ingested by the insect at larval stage has been recovered from the digestive tract and feces of adult flies. thus, the dissemination of the virus, even in a limited fashion, seems to be possible and attention is chiefly centered on the second process. | 1979 | 229988 |
| comparison of swine vesicular disease virus and coxsackie b5 virus by serological and rna hybridization methods. | the relatedness of swine vesicular disease virus (svdv) and coxsackie b5 virus has been studied by virus neutralization and immunodiffusion tests and by hybridization of the virus rnas. clearly defined differences between the two viruses were found by the three methods. isolates of svdv from several countries were very closely related but could be differentiated. recent isolates of coxsackie b5 virus also appeared to be similar but clear differences could be detected between these and the protot ... | 1976 | 58963 |
| structural proteins of swine vesicular disease virus. | 1975 | 163084 | |
| chemical inactivation of swine vesicular disease virus. | 1975 | 166725 | |
| the airborne excretion by pigs of swine vesicular disease virus. | the air of loose-boxes holding pigs affected with swine vesicular disease was sampled for virus. in the multistage impinger virus to a titre of 10(2.6) tcid 50 was associated with particles greater than 6 mum., 10(1.6) with particles 3-6 mum. and 10(1.4) or less with particles less than 3 mum. in the noses of workers in contact with the pigs for periods not less than 5 min., virus to a titre of 10(2.4) tcid 50 was found. virus was recovered from the air for 2-3 days during the disease and maximu ... | 1974 | 4361501 |
| airborne stability of swine vesicular disease virus. | 1974 | 4372779 | |
| swine vesicular disease: virus survival in pork products. | 1974 | 4377953 | |
| serological relationship of swine vesicular disease virus and coxsackie b5 virus. | 1973 | 4586437 | |
| rapid identification of swine vesicular disease virus in vesicular epithelium using the fluorescent antibody technique. | 1973 | 4365543 |