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Bacterial vaginosis and the natural history of human papillomavirus.To evaluate associations between common vaginal infections and human papillomavirus (HPV).201121869857
biomarkers of oxidant load and type-specific clearance of prevalent oncogenic human papillomavirus infection: markers of immune response?human papillomavirus (hpv) infection is the cause of cervical cancer. increased production of reactive oxygen species (ros) maybe the common mechanism through which hpv-cofactors (i.e., smoking and inflammation) influence duration of infections. biomarkers of total oxidant load may serve as cumulative measures of ros exposure due to these cofactors. therefore, we conducted a study evaluating the association between biomarkers of oxidant load and duration of hpv infections, early hpv natural hist ...201121858808
Usefulness of CINtec® PLUS p16/Ki-67 double-staining in cytological screening of cervical cancer.This study evaluated the usefulness of p16(INK4a)/Ki-67 as a new biomarker in the diagnosis of human papillomavirus (HPV)-related cervical lesions.201121986167
Feasibility of self-sampling and human papillomavirus testing for cervical cancer screening in First Nation women from Northwest Ontario, Canada: a pilot study.Background The incidence of cervical cancer is up to sixfold higher among First Nation women in Canada than in the general population. This is probably due to lower participation rates in cervical cancer prevention programmes. Objective To raise screening participation in this underserved population by launching an alternative approach to (Pap)anicolaou testing in a clinic-namely, vaginal self-sampling followed by human papillomavirus (HPV) diagnostics. Methods Good relationships were establis ...201122021733
project genesis: self-reported religiosity and spirituality and sexual risk-taking in young african-american women attending a historically african-american college.this pilot study explored the relationship between self-reported religiosity, spirituality, and sexual risk-taking. the participants were a convenience sample of (n = 100) female students attending a historically african-american college (hbcu) in the south. on this predominantly female campus, students completed an anonymous health-risk survey, plus additional items, to measure their religiosity and spirituality. correlation analysis revealed that although these students reported a high degree ...201121888148
human papillomavirus genotypes in iranian patients with cervical cancer.the aim of this study was to determine the frequency of hpv genotypes isolated from cervical intra-epithelial neoplasia grade iii and invasive carcinomas of iranian patients. a total of 94 cases were selected in five years from 2003 to 2007. after nucleic acid purification, real-time pcr was performed by means of gp5+/gp6+ primers. subsequently, pcr products were sequenced, on the basis of which a phylogenetic tree was constructed. negative samples and twelve randomly selected positive samples w ...201122041132
Tuberculosis and Oncogenic HPV: Potential Co-infections in Women at High-risk of Cervical Cancer in Rural China.The study was embedded in Shanxi Province Cervical Cancer Screening Study II with the aim of examining the association between history of diagnosed tuberculosis or cervical inflammation and oncogenic human papillomavirus (HPV) infection, persistent oncogenic HPV infection, cervical intraepithelial neoplasia grade 3 or cervical cancer (CIN3+) in an isolated rural population of China. A total of 8,798 women were recruited for cervical cancer screening and an interviewer-administered questionnaire. ...201122126473
Epidemiology and burden of HPV infection and related diseases: implications for prevention strategies.Human papillomavirus (HPV) infection is a necessary, although not sufficient cause of cervical cancer. Globally, HPV infection accounts for an estimated 530,000 cervical cancer cases (~270,000 deaths) annually, with the majority (86% of cases, 88% of deaths) occurring in developing countries. Approximately 90% of anal cancers and a smaller subset (<50%) of other cancers (oropharyngeal, penile, vaginal, vulvar) are also attributed to HPV. In total, HPV accounts for 5.2% of the worldwide cancer bu ...201121962466
Human papillomavirus in vaginal intraepithelial neoplasia.There are limited data on the prevalence and distribution of human papillomavirus (HPV) genotypes in vaginal intraepithelial neoplasia (VAIN). We sought to clarify this issue in a series of 450 VAIN cases with a confirmed diagnosis between 1990 and 2006. HPV genotyping was performed using paraffin-embedded specimens and polymerase chain reaction (PCR)-based methods. Multiple HPV types were validated by E6 type-specific PCR and direct sequencing. The HPV genotypes of the vaginal and cervical neop ...201122095387
Oral sex practices, oral human papillomavirus and correlations between oral and cervical human papillomavirus prevalence among female sex workers in Lima, Peru.Few data exist on oral human papillomavirus (HPV) prevalence in female sex workers (FSWs). Information regarding oral sex practices of 185 Peruvian FSWs, 18-26 years of age, was obtained via survey and compared with HPV testing results of oral rinse samples. Oral HPV prevalence was 14/185 (7.6%); four (28.9%) HPV genotypes were carcinogenic. One hundred and eighty-two participants reported having had oral sex; 95% reported condom use during oral sex with clients and 9.5% with partners. Women who ...201122096051
Eradication of large tumors expressing human papillomavirus E7 protein by therapeutic vaccination with E7 fused to the extra domain a from fibronectin.Cervical carcinoma is one of the most common cancers in women worldwide. It is well established that chronic infection of the genital tract by various mucosatropic human papillomavirus (HPV) types causes cervical cancer. Cellular immunity to E7 protein from HPV (HPVE7) has been associated with clinical and cytologic resolution of HPV-induced lesions. Thus, we decided to test if targeting of HPVE7 to dendritic cells using a fusion protein containing the extra domain A (EDA) from fibronectin, a na ...201121898393
[Detection of human papillomavirus in gingival fluid of Venezuelan HIV patients with periodontal disease].Evidence suggests that viruses may be involved in the activation of periodontal disease, allowing the overgrowth of periodontal pathogens. The purpose of the present study was to detect the presence of Human Papillomavirus (HPV) in gingival crevicular fluid (GCF) in HIV+ Venezuelan patients with periodontal disease. We evaluated GCF samples from 20 HIV+ patients with periodontal disease from the Infectious Disease Center, Faculty of Dentistry, Central University of Venezuela, and were clinically ...201121950192
diagnostic implications of l1, p16, and ki-67 proteins and hpv dna in low-grade cervical intraepithelial neoplasia.the expressions of p16, ki-67, and l1 proteins and human papillomavirus dna were investigated using polymerase chain reaction (hpv/pcr) and catalyzed signal-amplified colorimetric dna in situ hybridization (csac/ish) as potential molecular markers for the diagnosis and transforming potential of low cervical intraepithelial neoplasia (cin1). ki-67 and p16 protein expression increased linearly from control cases to more dysplastic cases (cin1, cin2, and cin3), peaking in squamous cell carcinoma ca ...201121979598
altered expression of claudin-1, claudin-7, and tricellulin regardless of human papilloma virus infection in human tonsillar squamous cell carcinoma.abstract conclusions: altered expression of claudin-1, claudin-7, and tricellulin in early tonsillar squamous cell carcinoma (scc) independent of human papilloma virus (hpv) infection may lead to tumor progression. objectives: integral tight junction proteins, the claudins and tricellulin, play a crucial role in all tissues. hpv is significantly associated with tonsillar scc. we sought to determine the expression of claudin-1, claudin-7, and tricellulin in hpv-infected and hpv-free tonsillar scc ...201121480761
cost-effectiveness of human papillomavirus vaccination and cervical cancer screening in thailand.please cite this paper as: sharma m, ortendahl j, van der ham e, sy s, kim j. cost-effectiveness of human papillomavirus vaccination and cervical cancer screening in thailand. bjog 2011; 10.1111/j.1471-0528.2011.02974.x. objective  to assess the health and economic outcomes of various screening and vaccination strategies for cervical cancer prevention. design  cost-effectiveness analysis from a societal perspective. setting  thailand. population  females aged 9 years and older. methods  using a ...201121481160
hpv vaccine acceptability in ghana, west africa.cervical cancer is a leading cause of cancer-related mortality among women in ghana. as of this writing no data are available concerning knowledge, attitudes and acceptability of human papillomavirus (hpv) vaccination by women in ghana.201121481327
design and characterization of an enhanced repressor of human papillomavirus e2 protein.papillomaviruses are causative agents of cervical and anogenital cancers. the viral e2 protein mediates viral dna replication and transactivation of viral oncogenes and thus represents a specific target for therapeutic intervention. short forms of e2, e2r, contain only the c-terminal dimerization domain, and repress the normal function of e2 due to formation of an inactive heterodimer. using structure-guided design, we replaced conserved residues at the dimer interface to design a heterodimer wi ...201121482558
advancing human papillomavirus research with a rhesus monkey model. 201121483022
immunogenicity and reactogenicity of alternative schedules of hpv vaccine in vietnam: a cluster randomized noninferiority trial.human papillomavirus (hpv) vaccine programs may decrease the morbidity and mortality due to cervical cancer seen among women in low-resource countries. however, the 3-dose schedule over a 6-month period is a potential barrier to vaccine introduction in such settings.201121486975
smoking initiation is followed by the early acquisition of epigenetic change in cervical epithelium: a longitudinal study.background:to prove a causal link between an epigenetic change and an environmental or behavioural risk factor for a given disease, it is first necessary to show that the onset of exposure precedes the first detection of that epigenetic change in subjects who are still free of disease.methods:towards this end, a cohort of women aged 15-19 years, recruited soon after they first had sexual intercourse, were used to provide sequential observations on the relationship between cigarette smoking and t ...201121487403
[current events in vaccination.]the annual meeting of the infectious disease society of america (idsa); which brought together nearly 5000 participants from over 80 countries in vancouver, canada, october 21 to 24, 2010; provided a review of the influenza (h1n1) 2009 pandemic, evaluated vaccination programmes and presented new vaccines under development. with 12 500 deaths in the united states in 2009-2010, the influenza (h1n1) 2009 pandemic was actually less deadly than the seasonal flu. but it essentially hit the young, and ...201121489733
transcriptome sequencing demonstrates that human papillomavirus is not active in cutaneous squamous cell carcinoma.β-human papillomavirus (β-hpv) dna is present in some cutaneous squamous cell carcinomas (cusccs), but no mechanism of carcinogenesis has been determined. we used ultra-high-throughput sequencing of the cancer transcriptome to assess whether papillomavirus transcripts are present in these cancers. in all, 67 cuscc samples were assayed for β-hpv dna by pcr, and viral loads were measured with type-specific quantitative pcr. a total of 31 sccs were selected for whole transcriptome sequencing. trans ...201121490616
medical interventions for high grade vulval intraepithelial neoplasia.vulval intraepithelial neoplasia (vin) is a pre-malignant condition of the vulval skin; its incidence is increasing in women under 50 years. vin is graded histologically as low grade or high grade. high grade vin is associated with infection with human papilloma virus (hpv) infection and may progress to invasive disease. there is no consensus on the optimal management of high grade vin. the high morbidity and high relapse rate associated with surgical interventions call for a formal appraisal of ...201121491403
impact of an hpv6/11/16/18 l1 virus-like particle vaccine on progression to cervical intraepithelial neoplasia in seropositive women with hpv16/18 infection.the impact of a human papillomavirus (hpv) vaccine on development of cervical intraepithelial neoplasia grade 2-3 or adenocarcinoma in situ (cin2-3/ais) in women with ongoing hpv16 or 18 infections prevaccination is reported. seventeen thousand six-hundred and twenty-two women aged 16-26 were enrolled in 1 of 2 randomized, placebo-controlled, efficacy trials (protocols 013 and 015). vaccine or placebo was given at day 1, month 2 and 6. women were tested for hpv6/11/16/18 dna and antibodies at da ...201121491420
the combined influence of oral contraceptives and human papillomavirus virus on cutaneous squamous cell carcinoma.the vast majority of cutaneous squamous cell carcinoma (cscc) will occur in those with fair complexion, tendency to burn, and high ultraviolet radiation (uvr) exposure. organ transplant recipients also are an important population at great risk for cscc. an association has been reported between oral contraceptive (oc) use, human papillomavirus virus (hpv) and cervical cancer, and there could be a similar association for cscc. the cutaneous hpv β-e6 protein, a close cousin of the transformative e6 ...201121499554
single-chain fv fragment antibodies selected from an intrabody library as effective mono- or bivalent reagents for in vitro protein detection.in spite of their many potential applications, recombinant antibody molecules selected by phage display are rarely available commercially, one reason being the absence of robust bacterial expression systems that yield sufficient quantities of reagents for routine applications. we previously described the construction and validation of an intrabody library that allows the selection of single-chain fv (scfv) fragments solubly expressed in the cytoplasm. here, we show that it is possible to obtain ...201121501618
detection of human papillomavirus using hybrid capture 2 in oral brushings from patients with oropharyngeal squamous cell carcinoma.detection of high-risk (hr) human papillomavirus (hpv) in oropharyngeal squamous cell carcinoma (scc) has important prognostic implications; patients exhibit improved survival compared with patients with hpv- scc. oral brushing and rinsing samples were obtained from patients with oropharyngeal, oral cavity, or hypopharyngeal scc and tested for hr-hpv using hybrid capture 2 (hc2; qiagen, valencia, ca). hr-hpv in situ hybridization (ish) was performed on biopsy tissue samples from the same patient ...201121502432
a population-based clinical trial comparing endocervical high-risk hpv testing using hybrid capture 2 and cervista from the shenccast ii study.our objective was to directly compare the accuracy of the high-risk human papillomavirus (hpv) assays, hybrid capture 2 (hc2; qiagen, gaithersburg, md) and cervista (hologic, bedford, ma), in diagnosing cervical intraepithelial neoplasia (cin) 3 or worse (cancer). a population-based, cross-sectional study (the shenzhen cervical cancer screening trial ii) was conducted in guangdong province in china. three high-risk hpv assays, self and direct cervical sampling and cytology, were studied. abnorma ...201121502436
phase 1 randomized trial of the vaginal safety and acceptability of spl7013 gel (vivagel) in sexually active young women (mtn-004).objectives:: the study was designed to assess the safety, adherence, acceptability, and effect on vaginal microflora of 3% spl7013 gel (vivagel), a novel dendrimer topical microbicide that inhibits hiv, herpes simplex virus-2, and human papillomavirus in vitro and in animal models. design:: phase 1, randomized, double-blind, placebo-controlled study on sexually active women. methods:: sixty-one sexually active women aged 18-24 years were recruited from three sites in the united states. participa ...201121505316
hpv e6 proteins target ubc9, the sumo conjugating enzyme.the human papillomavirus oncogenic protein, e6, interacts with a number of cellular proteins, and for some targets, e6 directs their degradation through the ubiquitin-proteasome pathway. post-translational modification with ubiquitin-like modifiers, such as sumo, also influences protein activities, protein-protein interactions, and protein stability. we report that the high risk hpve6 proteins reduce the intracellular quantity of the sole sumo conjugation enzyme, ubc9, concomitant with decreased ...201121510985
human papilloma virus genotype diversity of anal infection among trans (male to female transvestites, transsexuals or transgender) sex workers in argentina.reports on the prevalence and genotypes of hpv among trans (male to female transvestites, transsexuals or transgender) sex workers (tsw) are scarce in the literature.201121511521
multiplexed detection of nucleic acids in a combinatorial screening chip.multiplexed diagnostic testing has the potential to dramatically improve the quality of healthcare. simultaneous measurement of health indicators and/or disease markers reduces turnaround time and analysis cost and speeds up the decision making process for diagnosis and treatment. at present, however, most diagnostic tests only provide information on a single indicator or marker. development of efficient diagnostic tests capable of parallel screening of infectious disease markers could significa ...201121512691
"i thought cancer was one of those random things. i didn't know cancer could be caught…": adolescent girls' understandings and experiences of the hpv programme in the uk.the uk human papillomavirus (hpv) vaccination programme aims to provide girls aged 12-13 with protection against two of the most carcinogenic strains (types 16 and 18) of this sexually transmitted virus which together account for 70% of cases of cervical cancer. despite evidence suggesting a general lack of knowledge about hpv and its link with cervical cancer, vaccine uptake rates were generally high in the uk for the first year of the hpv vaccination programme. in countries that implemented th ...201121514348
anogenital warts in danish men who have sex with men.to determine the prevalence of anogenital warts (agws) and concurrent sexually transmitted infections (stis) in men who have sex with men (msm), and their knowledge of human papillomavirus (hpv). attitudes towards the hpv vaccine among msm are explored. a web-based cross-sectional survey on agws, sociodemographic factors and sexual behaviour conducted in august 2009 in denmark. overall 25.2% of the 1184 respondents reported a prior or current episode of agws. the prevalence of agw was significan ...201121515754
targeting hepatitis b virus and human papillomavirus induced carcinogenesis: novel patented therapeutics.viral infections leading to carcinogenesis tops the risk factors list for the development of human cancer. the decades of research has provided ample scientific evidence that directly links 10-15% of the worldwide incidence of human cancers to the infections with seven human viruses. moreover, the insights gained into the molecular pathogenetic and immune mechanisms of hepatitis b virus (hbv) and human papillomavirus (hpv) viral transmission to tumour progression, and the identification of their ...201121517743
human papillomavirus (hpv) prevalence and accuracy of hpv testing to detect high-grade cervical intraepithelial neoplasia.concern was raised on using testing for high-risk (hr) human papillomavirus (hpv) in cervical cancer screening in populations where hpv prevalence is high. the impact of hr hpv prevalence on the efficiency of hpv test-based screening has never been directly evaluated. a meta-regression of the relationship between hr hpv prevalence and the specificity and positive predictive value (ppv) of hpv dna testing for the presence of cervical intraepithelial neoplasia grade 2 or worse (cin2+) was performe ...201121520039
[whim syndrome: on the track of an interplay between human papillomavirus and the cxcl12 chemokine]. 201121524391
shedding of epstein-barr virus and cytomegalovirus from the genital tract of women in a peri-urban community in andhra pradesh, india.we found a large number of false-positive via (visual inspection with acetic acid) readings in a study of cervical cancer screening strategies (via, hpv dna testing, and pap cytology) in a peri-urban community in andhra pradesh, india. we evaluated whether these false-positives might be occurring as a result of infections with epstein-barr virus (ebv) or cytomegalovirus (cmv), prevalent latent herpesviruses known to be shed from the female genital tract. while we found that there was no associat ...201121525227
risks for persistence and progression by human papillomavirus type 16 variant lineages among a population-based sample of danish women.background: little is known about factors determining hpv16 persistence and progression, but several studies have suggested that genetic variants may play a role. methods: hpv16-positive women with normal cytology in a large danish cohort were re-assessed for hpv16 status at 2 years, and followed-up for cervical intraepithelial neoplasia 3 or worse (cin3+) over 11 years through linkage with a national pathology database. relative risks for clearance, persistence and progression were compared by ...201121527576
the prevalence of human papillomavirus in oral premalignant lesions and squamous cell carcinoma in comparison to cervical lesions used as a positive control.background: previous reports concerning the prevalence of human papillomavirus (hpv) in oral squamous cell carcinoma (oscc) have observed varied results. the aim of this study was to evaluate the prevalence of hpv in oral premalignant lesions (opl) and oscc. for accurate hpv detection in oral lesions, comparative analysis was performed on cervical lesions as positive controls. methods: fifty-seven cases with opl and 50 with oscc were selected. twenty-nine control cases were selected from cervica ...201121528380
nucleotide deficiency promotes genomic instability in early stages of cancer development.chromosomal instability in early cancer stages is caused by stress on dna replication. the molecular basis for replication perturbation in this context is currently unknown. we studied the replication dynamics in cells in which a regulator of s phase entry and cell proliferation, the rb-e2f pathway, is aberrantly activated. aberrant activation of this pathway by hpv-16 e6/e7 or cyclin e oncogenes significantly decreased the cellular nucleotide levels in the newly transformed cells. exogenously s ...201121529715
regulation of nucleocytoplasmic trafficking of viral proteins: an integral role in pathogenesis?signal-dependent targeting of proteins into and out of the nucleus is mediated by members of the importin (imp) family of transport receptors, which recognise targeting signals within a cargo protein and mediate passage through the nuclear envelope-embedded nuclear pore complexes. regulation of this process is paramount to processes such as cell division and differentiation, but is also critically important for viral replication and pathogenesis; phosphorylation appears to play a major role in r ...201121530593
the potential role of self-sampling for high-risk human papillomavirus detection in cervical cancer screening.high-risk human papillomavirus (hr-hpv) detection will become an important tool in the screening for cervical cancer. self-sampling is an inexpensive and well-accepted method for hpv detection that will increase participation of nonresponders in current screening programs. even more, because self-collected samples are as good as physician-collected samples for hpv detection, self-sampling might be a suitable method for future primary cervical cancer screening. copyright © 2011 john wiley & sons, ...201121538664
viruses and the fuel sensor: the emerging link between ampk and virus replication.adenosine 5' monophosphate-activated protein kinase (ampk) is conserved in all eukaryotic cells and functions as the key regulator of cellular metabolism by responding to the energy status of the cell. it is activated by an increase in the amp:atp ratio and then attempts to redress the balance by upregulating catabolic processes, whilst concomitantly inhibiting anabolic processes. despite its critical importance in the functioning of eukaryotic cells, there has been a paucity of studies investig ...201121538667
completion and timing of the three-dose human papillomavirus vaccine series among adolescents attending school-based health centers in oregon.many adolescents do not complete the 3-dose human papillomavirus vaccine series in the recommended time frame, or at all. given the challenges of administering a multi-dose vaccine to adolescents, especially those in vulnerable populations, we evaluated completion of the human papillomavirus vaccine series in 19 of oregon's school-based health centers.201121539853
long-term impact of human papillomavirus vaccination on infection rates, cervical abnormalities, and cancer incidence.: vaccination against human papillomavirus (hpv) types 16/18 is being implemented in many countries. there may be indirect benefit of hpv vaccination to nonvaccinated women, who may experience a reduced risk of infection with vaccine-preventable types (herd immunity). we attempt to disentangle the direct and indirect effects of hpv vaccination, while accounting for 14 oncogenic hpv types in a dynamic modeling framework.201121540743
polymorphisms of mdm4 and risk of squamous cell carcinoma of the head and neck.purpose: mouse double minute 4 (mdm4), a homolog of mdm2, is one of the key negative regulators of p53, and its amplification or overexpression contributes to carcinogenesis by inhibiting the p53 tumor suppressor activity. we investigated the association between mdm4 polymorphisms and the risk of squamous cell carcinoma of the head and neck (scchn). methods: we genotyped three mdm4 tagging polymorphisms, two in the 3' untranslated region (rs11801299g>a and rs10900598g>t) and one in intron 1 (rs1 ...201121540763
nrip, a novel calmodulin binding protein, activates calcineurin to dephosphorylate hpv e2 protein.previously, we found a gene named nuclear receptor interaction protein (nrip) (or dcaf6 or iqwd1). we demonstrate that nrip is a novel binding protein for human papillomavirus 16 (hpv-16) e2 protein. hpv-16 e2 and nrip can directly associate into a complex in vivo and in vitro, and the n-terminal domain of nrip interacts with the transactivation domain of hpv-16 e2. only full-length nrip can stabilize e2 protein and induce hpv gene expression, and nrip silenced by two designed sirnas decreases e ...201121543494
[prevalence of high- and low-risk oncogenic human papillomaviruses in patients with external genital pathology].the paper presents the results of examining 49 patients with genital papillomas, vulvar and vaginal leukoplakia, dysplasia, and cancer. the findings may suggest that human papillomavirus plays an important role in the development of vulvar and vaginal lesions and reconsider the importance of high- and low-risk oncogenic genotypes in the development of benign neoplasms, precancerous conditions, and malignant tumors of the vulva and vagina.201121545037
cervical human papilloma virus infection among the general female population in vietnam: a situation analysis.human papilloma virus (hpv) is the necessary cause of cervical cancer. this survey used a sample of 1,500 married women aged 15-69 to examine the prevalence of hpv infection and hpv specific types in vietnam as well as risk factors of hpv infection. results indicated that the prevalence of hpv infection in hanoi and hcm was 6.13 and 8.27. the proportion of multiple hpv infection was also higher in hcm than in hanoi (35.5% vs. 17.4%). risk factors having significant associations with general hpv ...201121545230
reported adverse events in girls aged 13-16 years after vaccination with the human papillomavirus (hpv)-16/18 vaccine in the netherlands.in 2009, human papillomavirus (hpv) vaccination was offered to girls born in 1993-1996 in a catch-up campaign, followed in 2010 by the implementation of the vaccination in the national immunization programme (nip) for girls born in 1997. to monitor the tolerability of the 2009 catch-up campaign, we investigated the occurrence of adverse events within 7 days after vaccination with the bivalent hpv vaccine. a total of 6000 girls were asked to participate, including 1500 from each birth cohort from ...201121549785
working to close the gap: identifying predictors of hpv vaccine uptake among young african american women.abstract:factors associated with greater likelihood of human papillomavirus (hpv) vaccination among women who experience excess cervical cancer incidence and mortality requires further study. the purpose of this study was to assess factors associated with hpv vaccine uptake among young african american women. three hundred sixty-three african american women aged 18-26 were recruited from historically black colleges/universities to complete a questionnaire to assess health beliefs associated with ...201121551933
considerations on the clinical application of the human papillomavirus vaccine in spain.the circumstances that in the opinion of the authors influence the lower than expected uptake of the vaccine against human papillomavirus in spain are analyzed. among others, aspects related to doubts about efficacy and safety, the attitude of the spanish health authorities or the role of media and the antivaccine groups, as well as the vaccination "culture" of the gynecologist, are discussed. the authors conclude that only with the coordinated effort of all involved bodies in the vaccination pr ...201121552002
the relationship of knowledge, health value and health self-efficacy with men's intentions to receive the human papillomavirus (hpv) vaccine.the aim of the present study was to determine whether it was possible to predict human papillomavirus (hpv) vaccination intentions an australian male sample. one hundred and twenty one males aged 18 to 26 (m = 22.3, sd = 2.06), completed an online survey assessing hpv-related knowledge, health beliefs and health self-efficacy. hpv knowledge and health self-efficacy were significant independent predictors of vaccine acceptance. a moderating effect was found between hpv knowledge and health self-e ...201121558104
prevalence and risk factors of human papillomavirus (hpv) infection in southern chinese women - a population-based study.persistent high-risk type human papillomavirus (hpv) infection is recognized as a necessary cause of cervical cancer. this study aimed to compare the hpv prevalence and risk factors between women residing in hong kong (hk) and guangzhou (gz) region of china.201121559276
clinical evaluation of polymerase chain reaction reverse hybridization assay for detection and identification of human papillomavirus type 16 variants.background: isolates of hpv16 comprise six variants: european (eu), asian (as), asian-american (aa), north american (na), african-1 (af1), and african-2 (af2) with different carcinogenic potentials. highly reliable automatable techniques for hpv variant genotyping would be helpful to confirm the role of variants in cervical cancer in large epidemiological studies. objective: to validate the performance of a novel assay for identification of hpv16 variants. study design: the test is a multiplex p ...201121561803
human papillomavirus infection in nasal polyps in a chinese population.in order to determine the prevalence and genotype distribution of human papillomavirus (hpv) infection in patients with nasal polyps, a total of 204 patients with nasal polyps and 36 healthy controls were recruited in this study. genomic deoxyribonucleic acid (dna) was extracted from paraffin-embedded tissue sections. hpv dna genotyping was achieved by a flow-through hybridization and gene chip method. hpv infection was positively identified in 82 of 204 (40.2%) patients, while hpv dna was not f ...201121562117
human papillomavirus vaccine introduction in low and middle income countries: guidance on the use of cost-effectiveness models.abstract: background: the world health organization recommends that the cost-effectiveness of introducing human papillomavirus (hpv) vaccination is considered before such a strategy is implemented. however, developing countries often lack the technical capacity to perform and interpret results of economic appraisals of vaccines. to provide information about the feasibility of using such models in a developing country setting, we evaluated models of hpv vaccination in terms of their capacity, req ...201121569406
genital human papillomavirus infection in patients with autoimmune inflammatory diseases.treatment advances achieved over the last few years have radically changed the management of patients with autoimmune inflammatory diseases requiring conventional or biological immunosuppressive therapy. these diseases and the drugs used to treat them increase the rate of infections, including genital infections due to the human papillomavirus (hpv). genital hpv infections have been extensively studied in organ transplant recipients, hiv-infected patients, and patients with congenital immune def ...201121570889
a protein profile study to discriminate cin lesions from normal cervical epithelium.background: cervical intraepithelial neoplasia (cin), a frequently encountered disease caused by human papilloma virus (hpv) is often diagnosed in formaldehyde-fixed paraffin embedded (ffpe) punch biopsies. since it is known that this procedure strongly affects the water-soluble proteins contained in the cervical tissue we decided to investigate whether a water-soluble protein-saving biopsy processing method can be used to support the diagnosis of normal and cin. methods: cervical punch biopsies ...201121573931
cancerous inhibitor of protein phosphatase 2a is overexpressed in cervical cancer and upregulated by human papillomavirus 16 e7 oncoprotein.objectives: cancerous inhibitor of protein phosphatase 2a (cip2a) is a recently identified oncoprotein stabilizing c-myc and promoting cell proliferation and transformation. here we investigated the role of cip2a in cervical cancer in vivo and in vitro. methods: cip2a expression was assessed in normal cervical, cervical intraepithelial neoplasia (cin) i to iii and cervical cancer tissues by immunohistochemistry and rt-pcr. cell growth was explored by cell proliferation assay, colony formation as ...201121575984
the impact of cytology screening and hpv vaccination on the burden of cervical cancer.aim:  to evaluate the impact of different strategies of human papillomavirus (hpv) vaccination on the burden of cervical cancer in singapore. methods:  the incidence of cervical cancer was calculated using a markov model with inputs based on singapore data for the prevalence of hpv infection, socioeconomic characteristics and screening prevalence. the evaluation was performed for 10 scenarios: no screening, current opportunistic cytology screening, ideal optimal screening, universal adolescent h ...201121585695
knowledge of pap smear, hpv and the hpv vaccine and the acceptability of the hpv vaccine by thai women.aims:  the primary prevention for cervical cancer, a human papilloma virus (hpv) vaccine, has been available in thailand for almost 3 years. the present study evaluates knowledge about the papanicolaou (pap) smear, hpv and the hpv vaccine and focuses on identifying predictors for the acceptability of the hpv vaccine. methods:  a sample of 764 women attending the gynecology clinic at ramathibodi hospital, bangkok, was asked to answer a questionnaire on their personal background, their knowledge o ...201121585696
should males be vaccinated against hpv? 201121587022
cytopathic effects of human papillomavirus infection and the severity of cervical intraepithelial neoplasia : a frequency study.cytopathic effects related to the human papillomavirus (hpv) infection are more frequently found in cervical intraepithelial neoplasia (cin) 1; however, there are indications that at least half the histological diagnoses of cin2 and cin3 include koilocytosis areas. the objective of this study was to evaluate the frequency of the cytological criteria suggestive of hpv infection in the cervical smears of women with a histological diagnosis of cin. one hundred and sixty-two women with abnormal cerv ...201121591275
differential survival trends for patients with tonsillar, base of tongue and tongue cancer in sweden.tonsillar, base of tongue and tongue cancer have similar anatomical and histopathological appearances but present differences in prognosis. human papillomavirus (hpv) is a known risk factor for tonsillar and base of tongue cancer, and a survival benefit has been shown for these tumors; however, hpv prevalence in tongue cancer is low. tonsillar, base of tongue and tongue cancer patients registered in the swedish cancer registry between 1960 and 2004 were followed from the date of cancer diagnosis ...201121592846
ccnd1 rs9344 polymorphisms are associated with the genetic susceptibility to cervical cancer in chinese population.cyclin d1, with a common g/a polymorphism in rs9344, is an essential regulator of the g1 phase in cell cycles and plays an important role in several tumor types, and the homology of cyclin d1 with human papillomavirus (hpv)-16 e7 brought our attention to ccnd1 gene in cervical cancer. a total of 738 native chinese subjects consist of 327 cases and 411 controls were enrolled in this study. ccnd1 genotyping was analyzed by polymerase chain reaction-restriction fragment length polymorphism (pcr-rfl ...201121594903
green and black tea in relation to gynecologic cancers.scope: observational studies have evaluated the relationship between green tea intake and cancers of the ovary and endometrium, but we are not aware of the published studies on green tea intake and risk of human papillomavirus (hpv)-related cancers of the cervix, vagina, or vulva. methods and results: a critical review of the published literature on tea intake and risk of ovarian and endometrial cancers was conducted. in meta-analyses, we report inverse associations for green tea intake and risk ...201121595018
molecular epidemiology of hpv infection using a clinical array methodology in 2952 women in greece.clin microbiol infect abstract: the molecular epidemiology of human papillomavirus (hpv) infection in a sample of greek women (n = 2952, mean age 42.2 ± 13.3 years) was examined. hpv prevalence was 50.7% (95% confidence interval, 48.8-52.6). the most frequent hpv types were hpv 53, 51 and 66 (10.2%, 9.4% and 9.3%, respectively). hpv positivity was associated with age, age of sexual debut, number of sexual partners and duration of sexual relationship, while marriage or multiparity protected again ...201121595788
[papillomavirus and head and neck cancer.]tobacco and alcohol consumption are the main risk factors for head and neck cancers. papillomavirus (hpv) infection was recently associated with the development of malignant tumors of the oropharynx, according to molecular and biological arguments. we describe the oncogenic mechanisms of hpv infections, the epidemiological and clinical aspects of associated head and neck cancers, their prognosis, and issues of specific therapeutic strategies.201121596407
a case of multiple squamous cell papillomas of the trachea.we report a case of 68-year-old woman suffering from breathlessness on exertion with stridor. a chest computed tomography showed a tumor arising from the posterior wall of the trachea. the diagnosis was squamous cell papilloma of the surgically removed tumor, which had caused the asphyxiation. after removal of the tumor, the patient received radical therapy: semiconductor laser transpiration. polymerase chain reaction (pcr) detected human papilloma virus (hpv) type 6, thought to be the cause of ...201121597426
[significance for the detection of human papillomavirus dna in cervical atypical glandular cells.]objective: to evaluate the potential link between high-risk human papilloma virus (hpv)dna testing and histological diagnosis in women referred for atypical glandular cells (agc) at pap smear. methods: all cervical cytological examinations with the diagnosis of agc between january 2007 and dec 2009 were identified by means of a computerized database in 97 patients. the histopathological results of cervix were analyzed by colposcopic biopsy, dilatation and curettage (d&c), cervical loop electrosu ...201121600120
knowledge of hpv in west virginia high school health students and the effects of an educational tool.study objective: to assess the knowledge of human papillomavirus (hpv) in west virginia high school students, create an educational dvd that could be used as a supplement to health class, and evaluate whether our intervention increases student knowledge of hpv. design: prospective, randomized experimental design with analysis of questionnaire data. setting: west virginia high school health class. participants: a total of 626 students participated. interventions: hpv educational dvd designed by h ...201121600809
follow-up outcomes for a large cohort of us women with negative imaged liquid-based cytology findings and positive high risk human papillomavirus test results.objective: this study aimed to follow a large group of us women with negative computer-imaged liquid-based cytology (lbc) and positive high risk (hr) hpv dna results. methods: negative lbc and positive hrhpv cases were identified between july 1, 2005 and december 31, 2009. cytologic and histopathologic follow-up results, repeat hpv results, and prior history were analyzed. results: 1099 patients with negative lbc and positive hrhpv results were identified. eight hundred sixty-nine had repeat pap ...201121601911
magnetic microparticle-based multiplexed dna detection with biobarcoded quantum dot probes.we have developed a new analytical method to detect multiple dna simultaneously based on the biobarcoded cdse/zns quantum dot (qd) and magnetic microparticle (mmp). it was demonstrated by using oligonucleotide sequences of 64 bases associated with human papillomavirus 16 and 18 l1 genes (hpv-16 and hpv-18) as model systems. this analytical system involves three types of probes, a mmp probe and two streptavidin-modified qd probes. the mmps are functionalized with hpv-16 and hpv-18 captures dna to ...201121602038
a long-term prospective study of type-specific human papillomavirus infection and risk of cervical neoplasia among 20,000 women in the portland kaiser cohort study.background. human papillomavirus (hpv) dna testing is more sensitive than cytology for detection of cervical intraepithelial neoplasia grade 3 and cancer (>cin3). adding hpv testing to cytology is recommended for women >30 but long-term prospective studies of hpv testing are rare. methods. beginning in 1989-1990, ~20,000 women in a pre-paid health maintenance organization (median age = 34) were followed passively by recommended annual cytology. we tested archived cervicovaginal lavage specimens ...201121602310
human papillomavirus testing in primary cervical screening and the cut-off level for hybrid capture 2 tests: systematic review.to determine the trade-off between the sensitivity and the specificity for high grade cervical intraepithelial neoplasia at hybrid capture 2 cut-off values above the standard ≥1 relative light units/cut-off level (rlu/co).201121606136
prevalence of human papillomavirus, epstein-barr virus, p21, and p53 expression in sinonasal inverted papilloma, nasal polyp, and hypertrophied turbinate in hong kong patients.background: the purpose of this study of human papillomavirus (hpv), epstein-barr virus (ebv), p21, and p53 in sinonasal inverted papilloma (ip) was to help elucidate its pathogenesis. methods: seventy-three ips, 48 nasal polyps, and 85 hypertrophied turbinates were subjected to hpv polymerase chain reaction (pcr) study. seventy-three ips, 30 nasal polyps, and 32 hypertrophied turbinates were subjected to ebv in situ hybridization (ish), p21, and p53 immunohistochemical (ihc) studies. results: h ...201121608063
[detection of human papilloma virus (hpv) in liquid-based cervical samples. correlation with protein p16ink4a expression].the liquid-based cervical cytology improves the quality of the sample and the residual sample could be used efficiently to carry out complementary tests, such as the detection of hpv dna and the immunocytochemical biomarkers study. the purpose of this study was to correlate the presence of hpv and immunoexpression of p16ink4a in liquid-based cervical samples to examine the utility of these new tools in the detection of cervical cancer. the included patients (n = 67) presented an abnormal cytolog ...201121614811
a systemic review of human papillomavirus studies: global publication comparison and research trend analyses from 1993 to 2008.the term "human papillomavirus" has been used as the keyword during searching titles, abstracts, and keywords based on the online version of science citation index (sci), web of science from 1993 to 2008. twelve document types were found among the 14,943 papers published in 1,072 journals that were listed in 99 sci subject categories. all the articles referring to human papillomavirus were assessed by using the following aspects: characteristics of publication output, distribution of output in j ...201121614898
human papillomavirus (hpv) vaccination: perception and practice among french general practitioners in the year since licensing.acceptance of the human papillomavirus (hpv) vaccine by targeted populations will depend to a large extent on its acceptability among physicians. we examined the perceptions, attitudes and practices of general practitioners (gps) in relation to hpv vaccination. from november 2007 to april 2008, a cross-sectional survey was carried out among a representative 5% sample of gps in the large rhône-alpes region of france. both quantitative (self-administered questionnaire) and qualitative (interview) ...201121616114
human papillomavirus genotypes among infected thai women with different cytological findings by analysis of e1 genes.cervical cytological data may not be sufficient for cervical cancer screening and prevention. in this project, we determined hpv genotype among infected thai women with different cytological findings by characterization of e1 genes. five hundred and thirty-five specimens were tested by pcr amplification of the e1 genes. hpv genotypes were determined by sequencing, comparison with the genbank database and were analyzed in relation to different cytological findings. hpv-dna by pcr were typed and r ...201121617826
distribution of human papillomavirus genotypes in paraguayan women according to the severity of the cervical lesion.the incidence of cervical cancer in paraguay is among the highest in the world. this study aimed to determine the distribution of human papillomavirus (hpv) genotypes in paraguayan women, according to the severity of the cervical lesion. this cross-sectional study included 207 women without a squamous intraepithelial lesion, 164 with low-grade squamous intraepithelial lesions, 74 with high-grade squamous intraepithelial lesions, and 41 with cervical cancer. type-specific hpv was determined by th ...201121618554
improved genetic association tests for an ordinal outcome representing the disease progression process.we are interested in detecting genetic variants that influence transition between discrete stages of a disease progression process, such as the natural history of progression to cervical cancer with the following four stages: (1) normal-human papillomavirus (hpv) exposed, (2) persistent infection with oncogenic hpv, (3) cervical intraepithelial neoplasia grades 2 or 3 (cin2/3), and (4) cervical cancer. standard statistical tests derived from the proportional odds model or polytomous regression m ...201121618605
hpv vaccine information-seeking behaviors among us physicians: government, media, or colleagues?the multiple information sources available may pose a challenge to physicians in providing accurate human papillomavirus (hpv) vaccine information to patients. the purpose of this study was to describe physicians' hpv vaccine information-seeking behaviors and assess if these behaviors differ by physician specialty and sociodemographic characteristics. in 2009, 1008 family physicians (fps), pediatricians (peds), and obstetricians/gynecologists (obgyns) completed a survey to assess their hpv vacci ...201121619906
clinicopathologic correlations between human papillomavirus 16 infection and beclin 1 expression in human cervical cancer.our earlier study showed that the autophagy gene beclin 1 could affect cell proliferation in a cervical cancer hela cell line. in this study, we examined beclin 1 protein expression in 81 specimens of cervical squamous carcinoma by immunohistochemistry. meanwhile, we detected e6 and e7 genes of human papillomavirus 16 in these tissues by polymerase chain reaction. beclin 1 expression significantly decreased in samples of malignant cervical cancer tissues than in those of normal or cervical intra ...201121623196
association between toll-like receptor expression and human papillomavirus type 16 persistence.the mechanisms involved in mucosal immune control of cervical human papillomavirus (hpv) infection remain ill defined. because toll-like receptors (tlrs) are key players in innate immune responses, we investigated the association between tlr expression and viral persistence or clearance in young women with incident infections with oncogenic hpv types 16 or 51. messenger rna expression of tlr1, tlr2, tlr3, tlr4, tlr6, tlr7, tlr8 and tlr9 was measured by quantitative reverse transcription-pcr usin ...201120473890
nf-κ b-dependent upregulation of icam-1 by hpv16-e6/e7 facilitates nk cell/target cell interaction.nk cell recognition of tumor cells is mediated by a delicate balance of signals received by mhc class i-binding inhibitory nk cell receptors and activating nk cell receptors, which mainly bind to virus-, stress- or tumor-induced ligands. in addition, adhesion molecules such as the intercellular adhesion molecule-1 (icam-1) and its receptors, the lymphocyte function-associated antigen-1 (lfa-1) and mac-1, are crucial for immune synapse formation and nk cell-mediated killing. in this study, we sho ...201120473930
a tritherapy combination of a fusion protein vaccine with immune-modulating doses of sequential chemotherapies in an optimized regimen completely eradicates large tumors in mice.tumor-induced immunosuppression plays a critical role in both impeding tumor-specific immune responses and limiting the effects of cancer immunotherapy. analyses of regulatory cells recruited during the growth of the e7-expressing tumor, tc-1, revealed a high percentage of regulatory t cells (tregs) as well as myeloid-derived suppressor cells (mdscs) in spleens and tumors. in this study, we proposed that treatment with immune-modulating doses of cyclophosphamide (ctx) and all-trans retinoic acid ...201120473939
awareness and knowledge about human papillomavirus among inuit women in nunavik, quebec.accurate knowledge about human papillomavirus (hpv) and its link to cervical cancer is essential for women to understand and make use of cervical cancer prevention and detection opportunities. this study was the first to survey awareness and knowledge of hpv in a population of canadian inuit. the objectives of this study were to assess the level of awareness and knowledge of inuit women in nunavik, quebec, canada, about hpv infection and its relation to cervical cancer and to study correlates of ...201120517640
trends in cervical cancer incidence and mortality in the baltic countries, bulgaria and romania.the burden of cervical cancer varies considerably in the european union (eu). in this article, we describe trends in incidence of and mortality from this cancer in the two most affected areas: the baltic countries (estonia, latvia and lithuania) and southeast europe (bulgaria and romania). incidence data were obtained from the national cancer registries. data on population and number of deaths from uterine cancers were extracted from the world health organization mortality database. mortality ra ...201120568103
immortalization of oral keratinocytes by functional inactivation of the p53 and prb pathways.a subgroup of head and neck squamous cell carcinomas (hnsccs) contains high-risk human papillomavirus-type 16 (hpv16). the viral e6 and e7 oncoproteins inactivate the p53 and prb proteins, respectively. we examined the causative effect of hpv16 e6 and e7 expression on the immortalization of normal oral keratinocytes (okcs) and compared the resulting phenotype with alternative ways of p53- and prb-pathway abrogation frequently found in hnsccs without hpv. primary okcs were conditionally immortali ...201120499310
perinatal outcomes in condyloma acuminata pregnancies.to investigate pregnancy outcome in patients with condyloma acuminata.201120556405
clustering of concordant and discordant cancer types in swedish couples is rare.spouses are exposed to common environmental cancer risk factors during adulthood. investigating the aggregation of cancer in couples might provide valuable insights into cancer development.201120655736
squamous cell carcinoma arising from human papillomavirus associated cyst. 201120661120
the acceptability of human papillomavirus vaccine among parents and guardians of newborn to 10-year-old children.the purpose of our study was to evaluate hpv vaccine acceptance among parents and guardians of children aged 0-10 years.201120709581
production and purification of human papillomavirus type 33 l1 virus-like particles from spodoptera frugiperda 9 cells using two-step column chromatography.the major capsid protein l1 of human papillomavirus (hpv) is essential in construction of recombinant antigen vaccines against cervical cancer. hpv type 33 accounts for about 10% of all hpv infections in asia. the gene encoding the major capsid protein l1 of the high-risk hpv type 33 was isolated from a korean patient and expressed in sf-9 insect cells using a baculovirus expression system. hpv33 l1 protein was isolated by two-step chromatographic purification using strong-cation exchange and ce ...201120716445
human papillomavirus and survival in patients with base of tongue cancer.the incidence of base of tongue cancer is increasing in sweden and the proportion of human papillomavirus (hpv) positive cancer has increased in stockholm, sweden. between 2006 and 2007, 84% of base of tongue cancer cases in stockholm were hpv-positive. the objective of this study was to assess the impact of hpv status on prognosis for base of tongue cancer patients. one-hundred and nine patients were diagnosed with base of tongue cancer between 1998 and 2007 in stockholm county and 95 paraffin- ...201120725995
[detection and quantification of human papillomavirus genital infections: virological, epidemiological, and clinical applications].human papillomaviruses (hpv) are the main risk factor for cervical cancer. by introducing its dna into the genome of infected human cells, the virus expresses two oncoproteins (e6 and e7) that induce inactivation of tumour suppressors and telomerase. hpv infection is extremely common. but most of women will clear the infection over an 8-10-month period without developing any cervical lesion. in fact, it is the persistence of infection that truly exposes to the risk of cervical cancer. hpv testin ...201120828954
randomized healthservices study of human papillomavirus-based management of low-grade cytological abnormalities.human papillomavirus (hpv)-based management of women with borderline atypical squamous cells of undetermined significance (ascus) or mildly abnormal cervical intraepithelial neoplasia (cini) cervical cytology has been extensively studied in the research setting. we wished to assess safety and health care resource use of a real-life health care policy using hpv triaging. all 15 outpatient clinics involved in the organized population-based screening program in stockholm, sweden screening program w ...201120824706
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