Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| an indirect elisa for serodiagnosis of cattle footrot caused by fusobacterium necrophorum. | a serodiagnostic elisa (rl-elisa) using recombinant truncated leukotoxin protein pl2 (aa 311-644) of fusobacterium necrophorum as antigen was developed for detection of antibodies against f. necrophorum from cattle footrot. in rl-elisa, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type a, bovine escherichia coli, and bovine salmonella. the rl-elisa ... | 2010 | 20304080 |
| effect of foot-and-mouth disease virus capsid precursor protein and 3c protease expression on bovine herpesvirus 1 replication. | several reports have previously shown that expression of the foot-and-mouth disease virus (fmdv) capsid precursor protein encoding region p1-2a together with the 3c protease (p1-2a/3c) results in correct processing of the capsid precursor into vp0, vp1 and vp3 and formation of fmdv capsid structures that are able to induce a protective immune response against fmdv challenge after immunization using naked dna constructs or recombinant viruses. to elucidate whether bovine herpesvirus 1 (bhv-1) mig ... | 2010 | 20333533 |
| bighorn sheep fetal lung cell line for detection of respiratory viruses. | pneumonia is an important disease of bighorn sheep (bhs) that is primarily responsible for the drastic decline in numbers of these animals in north america. members of the genus mannheimia and pasteurella have frequently been isolated from the pneumonic lungs of bhs. antibodies to several respiratory viruses, including bovine parainfluenza virus 3 (bpiv-3), bovine respiratory syncytial virus (brsv), bovine viral diarrhea virus (bvdv), and bovine herpesvirus 1 (bohv-1), have been detected in herd ... | 2010 | 20357964 |
| immunogenicity of a modified-live virus vaccine against bovine viral diarrhea virus types 1 and 2, infectious bovine rhinotracheitis virus, bovine parainfluenza-3 virus, and bovine respiratory syncytial virus when administered intranasally in young calves. | the immunogenicity of an intranasally-administered modified-live virus (mlv) vaccine in 3-8 day old calves was evaluated against bovine viral diarrhea virus (bvdv) types 1 and 2, infectious bovine rhinotracheitis (ibr) virus, parainfluenza-3 (pi-3) virus and bovine respiratory syncytial virus (brsv). calves were intranasally vaccinated with a single dose of a multivalent mlv vaccine and were challenged with one of the respective viruses three to four weeks post-vaccination in five separate studi ... | 2010 | 20381643 |
| erratum to: serological evidence of bovine herpesvirus-1 (bohv-1) infection in yaks (peophagus grunniens) from the national research centre on yak, india. | 2010 | 20390352 | |
| productive infection and bicp0 early promoter activity of bovine herpesvirus 1 are stimulated by e2f1. | bovine herpesvirus 1 (bohv-1) is an important viral pathogen of cattle. like other members of the subfamily alphaherpesvirinae, bohv-1 establishes latency in sensory neurons and has the potential to reactivate from latency. dexamethasone (dex) treatment of latently infected calves or rabbits consistently leads to reactivation from latency. the bohv-1 transcript encoding the infected cell protein 0 (bicp0) is consistently detected during reactivation from latency, in part because the bicp0 early ... | 2010 | 20410283 |
| small noncoding rnas encoded within the bovine herpesvirus 1 latency-related gene can reduce steady-state levels of infected cell protein 0 (bicp0). | following acute infection in mucosal epithelium, bovine herpes virus 1 (bhv-1) establishes lifelong latency in sensory neurons within trigeminal ganglia. the latency-related rna (lr-rna) is abundantly expressed in sensory neurons of latently infected calves. expression of lr proteins is necessary for the latency reactivation cycle because a mutant virus that does not express lr proteins is unable to reactivate from latency after dexamethasone treatment. lr-rna sequences also inhibit bicp0 expres ... | 2010 | 20410286 |
| intracellular trafficking of vp22 in bovine herpesvirus-1 infected cells. | the intracellular trafficking of different vp22-enhanced yellow fluorescent protein (eyfp) fusion proteins expressed by bovine herpesvirus-1 (bhv-1) recombinants was examined by live-cell imaging. our results demonstrate that (i) the fusion of eyfp to the c terminus of vp22 does not alter the trafficking of the protein in infected cells, (ii) vp22 expressed during bhv-1 infection translocates to the nucleus through three different pathways, namely early mitosis-dependent nuclear translocation, l ... | 2010 | 19922972 |
| neutralizing antibodies to bovine herpesvirus types 1 (bohv-1) and 5 (bohv-5) and its subtypes. | this study was carried out to determine whether the sensitivity of serum neutralization (sn) tests would be affected by the use of distinct subtypes of bovine herpesvirus 1 (bohv-1) and 5 (bohv-5) as test challenge viruses. bovine sera collected from a randomized sample (n=287) were tested in a 24h incubation sn against three type 1 viruses (bohv-1.1 strains "los angeles" (la) and "evi 123"; bohv-1.2a strain "sv 265") and three type 5 viruses (bohv-5a strain "evi 88"; bohv-5b strain "a 663" and ... | 2010 | 19926411 |
| characterization of micrornas encoded by the bovine herpesvirus 1 genome. | bovine herpesvirus 1 (bohv-1) is a ubiquitous and important pathogen of cattle worldwide. this study reports the identification of 10 microrna (mirna) genes, bhv1-mir-b1-bhv1-mir-b10, encoded by the bohv-1 genome that were processed into 12 detectable mature mirnas as determined by ultra-high throughput sequencing bioinformatics analyses of small rna libraries and expression studies. we found that four of the mirna genes were present as two copies in the bohv-1 genome, resulting in a total of 14 ... | 2010 | 19793906 |
| a ul47 gene deletion mutant of bovine herpesvirus type 1 exhibits impaired growth in cell culture and lack of virulence in cattle. | tegument protein vp8 encoded by the u(l)47 gene of bovine herpesvirus type 1 (bhv-1) is the most abundant constituent of mature virions. in the present report, we describe the characterization of u(l)47 gene-deleted bhv-1 in cultured cells and its natural host. the u(l)47 deletion mutant exhibited reduced plaque size and more than 100-fold decrease in intracellular and extracellular viral titers in cultured cells. ultrastructural observations of infected cells showed normal maturation of bhv-1 v ... | 2010 | 19864376 |
| bovine herpesvirus type 1 as a novel oncolytic virus. | oncolytic virotherapy is a promising avenue of cancer gene therapy. current vectors include human viruses that have been engineered to replicate in tumor cells or nonhuman viruses that are naturally oncotropic and preferentially replicate in tumor cells harboring defects in innate immune pathways such as the type 1 interferon (ifn) pathway. bovine herpesvirus type 1 (bhv-1) is a species-specific herpesvirus closely related to the human herpes simplex virus type 1 (hsv-1). although bhv-1 does not ... | 2010 | 19893594 |
| prevalence of brucellosis and infectious bovine rhinotracheitis in organized dairy farms in india. | this study was carried out to investigate the prevalence of bovine brucellosis and infectious bovine rhinotracheitis (ibr) in organized dairy farms with history of abortion in india. elisa and rose bengal plate test (rbpt) were used to detect the seropositive animals and the test results indicated that 22.18% and 13.78% animals were declared as sero-positive by elisa and rbpt, respectively. milk ring test (mrt) was carried out only in one farm and 12.82% of the tested animals were turned positiv ... | 2010 | 19644761 |
| seroconversion to bovine viral diarrhoea virus and infectious bovine rhinotracheitis virus in dairy herds of michoacan, mexico. | bovine viral diarrhoea virus (bvdv) and infectious bovine rhinotracheitis virus (ibrv) are important viral diseases around the world. the objective of this study was to estimate the incidence of seroconversion to bvdv and ibrv and to identify associated risk factors in dairy herds of michoacan, mexico. the longitudinal study included 62 herds and ran from december 2001 to november 2002. the total number of animals enrolled and completing the study were 392 and 342 animals for bvdv and 925 and 89 ... | 2010 | 19685278 |
| bovine herpesvirus: what is missing from our understanding of the relationship between bohv-1 and bohv-5? | 2010 | 19703787 | |
| biology of bovine herpesvirus 5. | bovine herpesvirus 5 (bohv-5) is an alphaherpesvirus responsible for meningoencephalitis in young cattle and is closely antigenically and genetically related to bovine herpesvirus 1 (bohv-1). both viruses have common aspects in their pathogenesis: (1) they infect epithelial cells at the portal of entry and (2) they establish a latent infection in the sensory nerve ganglia, i.e., the trigeminal ganglia. however, they have different neuroinvasion and neurovirulence capacities. only in rare cases c ... | 2010 | 19409823 |
| 2,3,7,8-tetrachlorodibenzo-p-dioxin modifies expression and nuclear/cytosolic localization of bovine herpesvirus 1 immediate-early protein (bicp0) during infection. | our previous studies have demonstrated that 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) increases bovine herpesvirus 1 (bhv-1) replication through a dose-dependent increase in cytopathy and increased viral titer. furthermore, tcdd was able to trigger bhv-1-induced apoptosis by up-regulating the activation of initiator caspases 8 and 9, as well as of effector caspase 3. since tcdd activates caspase 3 after 4 h of infection, we have hypothesized an involvement of bhv-1 infected cell protein 0 (bicp ... | 2010 | 20506271 |
| recombinant n-terminal part of bovine herpesvirus 1 icp27 protein: its preparation, purification, and use for raising specific antiserum. | recombinant n-terminal part of bovine herpesvirus-1 (bohv-1) icp27 protein fused with thioredoxin and his-tag ("the recombinant protein") expressed in escherichia coli was purified by the ni2+-nta affinity chromatography and used for the preparation of antiserum by immunization of rabbits. the antiserum recognized the recombinant protein in western blot analysis and was able to detect bohv-1 icp27 in the nucleoli of bohv-1-infected mdbk cells. these results showed that such an antiserum could se ... | 2010 | 20545445 |
| seroepidemiology of bovine herpesvirus 1 (bhv1) infection among estonian dairy herds and risk factors for the spread within herds. | the objectives of this study were to reassess the herd level and within-herd prevalence of bovine herpesvirus 1 (bhv1) infection in estonian dairy cattle, estimate the sensitivity and specificity of the enzyme-linked immunosorbent assay (elisa) for bulk tank milk (btm) testing and determine the risk factors related to high prevalence of the infection in herds. to estimate the herd prevalence, btm samples from each of the 1,205 herds that sell milk to dairy companies were analysed for bhv1 antibo ... | 2010 | 20598386 |
| immunization and challenge experiments with a new modified live bovine herpesvirus type 1 marker vaccine prototype adjuvanted with a co-polymer. | western european control programs against bovine herpesvirus type 1 (bohv-1) infections utilize attenuated bohv-1 marker vaccines with a deletion of the glycoprotein e (ge) encoding gene. however, a recent study demonstrated the potential risk of virulence recovery of ge-deleted bohv-1 marker vaccine strains due to recombination (muylkens et al. [15]). based on an infectious bacterial artificial chromosome clone, a ge- and thymidine kinase (tk)-gene-deleted bohv-1 mutant (bohv-1deltagedeltatk) w ... | 2010 | 20600486 |
| a comparative analysis of envelope and tegument proteins of suid herpesvirus 1, bovine herpesvirus 1 and bovine herpesvirus 5. | the aim of this study was to analyze the disordered regions (drs) in the envelope and tegument proteins of three closely related herpesviruses: bovine herpesvirus 1, bovine herpesvirus 5 and suid herpesvirus 1. tegument proteins showed a greater percentage of drs than the envelope proteins did. regions of disorder were found in the less conserved portions of the proteins, n-terminal and c-terminal regions, and, in a few cases, functionally important sites. the presence of drs is an important fac ... | 2010 | 20607317 |
| bovine herpesvirus type 1 (bhv-1) is an important cofactor in the bovine respiratory disease complex. | bhv-1 is an important pathogen of cattle. because of its ability to induce immune suppression, bhv-1 is an important agent in the multifactorial disorder, bovine respiratory disease complex (brdc). bhv-1 encodes several proteins that inhibit various arms of the immune system suggesting that these proteins are important in the development of brdc. | 2010 | 20619186 |
| validation of an indirect elisa to detect antibodies against bohv-1 in bovine and guinea-pig serum samples using iso/iec 17025 standards. | two elisas to quantify antibodies to bohv-1 in the sera of cattle and immunized guinea pigs were developed and validated using iso/iec 17025 standards. the cut-off value of the assay was established at 20% positivity of a high positive control for screening of cattle. using this threshold, the assay properly classified the oie bovine reference sera eu1, eu2 and eu3. for vaccine potency testing, a cut-off of 40% was selected for both species. the reliability of the assays, given by their diagnost ... | 2010 | 20655331 |
| antibodies to pathogenic livestock viruses in a wild vicuña (vicugna vicugna) population in the argentinean andean altiplano. | serum samples from 128 wild vicuñas (vicugna vicugna) were tested for antibodies (ab) to rotavirus (rv), bovine parainfluenza virus 3 (bpiv-3), bovine herpesvirus-1 (bhv-1), bovine viral diarrhea virus (bvdv-1), foot-and-mouth disease virus (fmdv), bluetongue virus (btv), equine herpesvirus-1 (ehv-1), and influenza a virus equine (eiv). samples were collected in cieneguillas province of jujuy, in northern argentina. feces from 44 vicuñas were also collected to investigate rv shedding. llamas (la ... | 2010 | 20688660 |
| abortifacient vaccines and bovine herpesvirus-1. | 2010 | 20712064 | |
| microbiological and pathological examination of fatal calf pneumonia cases induced by bacterial and viral respiratory pathogens. | the infectious origin of fatal cases of calf pneumonia was studied in 48 calves from 27 different herds on postmortem examination. lung tissue samples were examined by pathological, histological, bacterial culture, virus isolation and immunohistochemical methods for the detection of viral and bacterial infections. pneumonia was diagnosed in 47/48 cases and infectious agents were found in 40/47 (85%) of those cases. the presence of multiple respiratory pathogens in 23/40 (57.5%) cases indicated t ... | 2010 | 20713325 |
| water-extracted polysaccharides from azadirachta indica leaves: structural features, chemical modification and anti-bovine herpesvirus type 1 (bohv-1) activity. | in this study, we have analyzed the pectic arabinogalactan isolated from azadirachta indica and its chemically sulfated derivative. the native polysaccharide had an apparent molecular mass of 80 kda and is made up of (1→5)-/(1→3,5)-linked α-l-arabinosyl, (1→3)-/(1→6)-/(1→3,6)-linked β-d-galactosyl, and terminal-rhamnosyl and α-l-arabinosyl residues. these macromolecules showed activity against bovine herpesvirus type-1. their inhibitory concentration 50% values ranging from 31.12 to 105.25 μg/ml ... | 2010 | 20732349 |
| chitosan based delivery systems for mucosal immunization against bovine herpesvirus 1 (bhv-1). | bovine herpesvirus 1 (bhv-1), is a major pathogen of cattle which causes serious infections, including infectious bovine rhinotracheitis (ibr)/infectious pustular vulvovaginitis (ipv). at present, bhv-1 is still a serious threat to animal health and productivity in turkey, hence to develop a more efficient and economical vaccine system against bhv-1 is certainly an important necessity. a mucosal vaccination strategy would provide both mucosal and systemic immune responses to protect disease prog ... | 2010 | 20800678 |
| prevalence of pasteurella multocida and other respiratory pathogens in the nasal tract of scottish calves. | the prevalence of pasteurella multocida, a cause of bovine respiratory disease, was studied in a random sample of beef suckler and dairy farms throughout scotland, by means of a cross-sectional survey. a total of 637 calves from 68 farms from six geographical regions of scotland were sampled between february and june 2008. deep nasal swabs were taken, and samples that were culture-positive for p multocida were confirmed by pcr. prevalence of p multocida was 17 per cent (105 of 616 calves); 47 pe ... | 2010 | 21257416 |
| indications of a relationship between buying-in policy and infectious diseases on dairy farms in wales. | during the period february to may 2008, bulk milk samples were collected from 57 dairy farms throughout wales in the framework of a voluntary somatic cell count project. bulk milk samples were tested for antibodies to bovine viral diarrhoea virus (bvdv), bovine herpesvirus type 1 (bhv-1) and leptospira hardjo, and samples were also tested for the presence of bvdv antigen by pcr. a questionnaire was used to determine whether the herd was open or closed, what the vaccination status was, and to obt ... | 2010 | 21257463 |
| detection of bovine herpesvirus 1 sequences in yaks (bos grunniens) with keratoconjunctivitis, using a highly sensitive nested polymerase chain reaction. | thirty-seven yaks (bos grunniens) with keratoconjunctivitis and 22 healthy yaks were used to investigate the role of bovine herpesvirus 1 (bohv-1) in keratoconjunctivitis in yaks. nucleic acid sequences of bohv-1 glycoproteins b and e were detected in conjunctival swabs from all yaks with keratoconjunctivitis using a nested polymerase chain reaction (pcr). in 21 yaks, bohv-1 sequences were detected along with moraxella bovis (m. bovis) and neisseria spp. the amplified bohv-1 sequences were ident ... | 2010 | 21309468 |
| regulation of innate immune responses by bovine herpesvirus 1 and infected cell protein 0 (bicp0). | bovine herpesvirus 1 (bohv-1) infected cell protein 0 (bicp0) is an important transcriptional regulatory protein that stimulates productive infection. in transient transfection assays, bicp0 also inhibits interferon dependent transcription. bicp0 can induce degradation of interferon stimulatory factor 3 (irf3), a cellular transcription factor that is crucial for activating beta interferon (ifn-ß) promoter activity. recent studies also concluded that interactions between bicp0 and irf7 inhibit tr ... | 2009 | 21994549 |
| comparison of the pseudorabies virus us9 protein with homologs from other veterinary and human alphaherpesviruses. | pseudorabies virus (prv) us9 is a small, tail-anchored (ta) membrane protein that is essential for axonal sorting of viral structural proteins and is highly conserved among other members of the alphaherpesvirus subfamily. we cloned the us9 homologs from two human pathogens, varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1), as well as two veterinary pathogens, equine herpesvirus type 1 (ehv-1) and bovine herpesvirus type 1 (bhv-1), and fused them to enhanced green fluorescent ... | 2009 | 19420087 |
| critical assessment of an in vitro bovine respiratory organ culture system: a model of bovine herpesvirus-1 infection. | a bovine in vitro organ culture (bivoc) system was evaluated as a model to study host and pathogen events during the course of bovine herpesvirus-1 infection. upper respiratory tract epithelium, from slaughtered animals, was cultured in an air-liquid interface system and integrity, viability, and tnf-alpha gene expression of tissue explants were monitored over 72h in the presence or absence of infection by bovine herpesvirus type 1 (bhv-1). uninfected explants maintained viability and integrity ... | 2009 | 19428580 |
| natural exposure of dromedary camels in sudan to infectious bovine rhinotracheitis virus (bovine herpes virus-1). | the occurrence of bovine herpes virus-1 (bhv-1) in camels was studied. a total of 186 pneumonic camel lungs were collected from slaughter houses at four different areas in sudan during 2000-2006. using sandwich elisa 1.6% of 186 tested lungs were found positive for bhv-1 antigen, all were from tambool at central sudan. direct fluorescent antibody test (fat) was used to confirm the bhv-1 elisa positives, all elisa positives were also positive. pcr was used to detect bhv-1 genome with three positi ... | 2009 | 19433051 |
| characterization of interspecific recombinants generated from closely related bovine herpesviruses 1 and 5 through multiple pcr sequencing assays. | bovine herpesviruses 1 (bohv-1) and 5 (bohv-5) are closely related alphaherpesviruses infecting cattle. in countries where both viruses circulate, co-infection of cattle is likely. it was shown that recombination occurs at a high frequency in cattle infected dually with two bohv-1 mutants. in addition, interspecific recombinants are generated in cell culture co-infected with bohv-1 and bohv-5. even if the process of interspecific recombination appears inefficient relative to intraspecific recomb ... | 2009 | 19501619 |
| dexamethasone treatment of calves latently infected with bovine herpesvirus 1 leads to activation of the bicp0 early promoter, in part by the cellular transcription factor c/ebp-alpha. | sensory neurons within trigeminal ganglia (tg) are the primary site for bovine herpesvirus 1 (bhv-1) latency. during latency, viral gene expression is restricted to the latency-related (lr) gene and the open reading frame orf-e. we previously constructed an lr mutant virus that expresses lr rna but not any of the known lr proteins. in contrast to calves latently infected with wild-type (wt) bhv-1 or the lr rescued virus, the lr mutant virus does not reactivate from latency following dexamethason ... | 2009 | 19553330 |
| bovine herpes virus infections in cattle. | bovine herpes virus 1 (bhv-1) is primarily associated with clinical syndromes such as rhinotracheitis, pustular vulvovaginitis and balanoposthitis, abortion, infertility, conjunctivitis and encephalitis in bovine species. the main sources of infection are the nasal exudates and the respiratory droplets, genital secretions, semen, fetal fluids and tissues. the bhv-1 virus can become latent following a primary infection with a field isolate or vaccination with an attenuated strain. the viral genom ... | 2009 | 19558751 |
| high prevalence of co-infections with bovine herpesvirus 1 and 5 found in cattle in southern brazil. | based on small scale studies or on little sensitive serological tests, bovines in the south of the state of rio grande do sul, brazil, are known to be infected with either bovine herpesvirus 1 (bohv-1) or 5 (bohv-5). however, whether the prevalence of each of these viruses is high or low is currently still unknown. in order to determine the extent of bohv (-1 and/or -5) infections in bovines in this region of brazil, 200 bovines were studied for the presence of bohv dna. to this end, first a qua ... | 2009 | 19560292 |
| lung pathology and infectious agents in fatal feedlot pneumonias and relationship with mortality, disease onset, and treatments. | this study charted 237 fatal cases of bovine respiratory disease (brd) observed from may 2002 to may 2003 in a single oklahoma feed yard. postmortem lung samples were used for agent identification and histopathology. late in the study, 94 skin samples (ear notches) were tested for bovine viral diarrhea virus (bvdv) by immunohistochemistry (ihc). bovine respiratory disease morbidity was 14.7%, and the mortality rate of all causes was 1.3%, with more than half (53.8%) attributed to brd (0.7% total ... | 2009 | 19564494 |
| evaluation of a blocking enzyme-linked immunosorbent assay for serological diagnosis of bovine herpesvirus 1. | bovine herpesvirus 1 (bohv-1) is an infectious agent of concern in the international export of bovine products; it is endemic in the united states, but it has been eradicated in many countries of the european union (eu). for export of semen to the eu, accurate assessment of bohv-1 status of the bull is required and is usually accomplished by measuring the level of antibody to the virus. the gold standard is virus neutralization (vn) using overnight incubation with the virus, a test approved by t ... | 2009 | 19564503 |
| serological response in cattle immunized with inactivated oil and algel adjuvant vaccines against infectious bovine rhinotracheitis. | infectious bovine rhinotracheitis (ibr) virus was grown in madin darby bovine kidney (mdbk) cell line using a roller culture system for its large-scale production. optimum multiplicity of infection (moi) of 1:750 was found to give consistent virus yield. to determine the appropriate payload, three batches of antigen with virus titres ranging from 10(8.37) to 10(6.37) tcid50 per ml were used to prepare experimental inactivated ibr oil adjuvant vaccine. beta-propiolactone (bpl) was used as inactiv ... | 2009 | 19579689 |
| alphaherpesvirus infections in semidomesticated reindeer: a cross-sectional serological study. | alphaherpesviruses infect a wide range of animal species and cause diseases. cervid herpesvirus 2 (cvhv-2) was originally isolated from reindeer in finland but the impact of cvhv-2 infections on reindeer remains unclear. cvhv-2 infection could be partly responsible for calf losses as there are indications that it is associated with abortions and neonatal diseases. previous serosurveys of reindeer (rangifer tarandus tarandus) have shown that an alphaherpesvirus is circulating among reindeer in no ... | 2009 | 19604658 |
| effect of cryopreservation by slow cooling and vitrification on viral contamination of ivf embryos experimentally exposed to bovine viral diarrhea virus and bovine herpesvirus-1. | the objective was to determine the effect of cryopreservation by conventional slow controlled cooling (0.5 degrees c/min) and by vitrification on the presence of bovine viral diarrhea virus (bvdv) and bovine herpesvirus-1 (bhv-1) infectivity associated with frozen-thawed day 7 bovine embryos. in this study, day 7 embryos generated by in vitro fertilization (ivf) were exposed in vitro for 1.5h to bvdv (n=393) and bhv-1 (n=242) and subsequently tested before and after cryopreservation for the pres ... | 2009 | 19616294 |
| infertility and venereal disease in cattle inseminated with semen containing bovine herpesvirus type 5. | a group of 20 cows and heifers experienced poor conception rates and probable ovarian dysfunction after being artificially inseminated. when first examined, some showed signs of vulvovaginitis, with pustular, ulcerative lesions consistent with a herpesvirus infection. they had had no contact with bulls during the current breeding season. bovine herpesvirus type 5 (bhv-5) was isolated from samples of frozen semen from the batch that had been used for the artificial insemination programme. bhv-5 w ... | 2009 | 19633324 |
| repertoire of bovine mirna and mirna-like small regulatory rnas expressed upon viral infection. | microrna (mirna) and other types of small regulatory rnas play a crucial role in the regulation of gene expression in eukaryotes. several distinct classes of small regulatory rnas have been discovered in recent years. to extend the repertoire of small rnas characterized in mammals and to examine relationship between host mirna expression and viral infection we used illumina's ultrahigh throughput sequencing approach. we sequenced three small rna libraries prepared from cell line derived from the ... | 2009 | 19633723 |
| [construction and identification of recombinant bhv-1 expressing foot and mouth disease virus vp1 gene]. | in order to construct the recombinant bovine hepervirus-1 (bhv-1) which expressed foot and mouth disease virus (fmdv) vp1 gene, we constructed a bhv-1 ge gene transfer vector by inserting the synthetic vp1 gene of fmdv (o/china/99) under the immediate-early promoter of cytomegalovirus. | 2009 | 19637579 |
| efficacy of a combination viral vaccine for protection of cattle against experimental infection with field isolates of bovine herpesvirus-1. | to determine whether a combination viral vaccine containing a modified-live bovine herpesvirus-1 (bhv-1) would protect calves from infection with virulent field strains of bhv-1 for weeks or months after vaccination. | 2009 | 19719449 |
| comparison of single vaccination versus revaccination with a modified-live virus vaccine containing bovine herpesvirus-1, bovine viral diarrhea virus (types 1a and 2a), parainfluenza type 3 virus, and bovine respiratory syncytial virus in the prevention of bovine respiratory disease in cattle. | objective-to compare effects of administration of a modified-live respiratory virus vaccine once with administration of the same vaccine twice on the health and performance of cattle. design-randomized, controlled trial. animals-612 mixed-breed male cattle with unknown health histories. procedures-cattle were randomly assigned to 1 of 2 treatment groups (single vaccination treatment group [svac group] vs revaccination treatment group [revac group]) during the preconditioning phase of production. ... | 2009 | 19719452 |
| major tegument protein vp8 of bovine herpesvirus 1 is phosphorylated by viral us3 and cellular ck2 protein kinases. | the ul47 gene product, vp8, is one of the major tegument proteins of bovine herpesvirus 1 (bohv-1) and is subject to phosphorylation. analysis of protein bands co-immunoprecipitated with vp8 from bohv-1-infected cells by mass spectroscopy suggested that vp8 interacts with two protein kinases: cellular ck2 and viral us3. ck2 is a highly conserved cellular protein, expressed ubiquitously and known to phosphorylate numerous proteins. the us3 gene product is one of the viral kinases produced by bohv ... | 2009 | 19692545 |
| characterization of the nuclear and nucleolar localization signals of bovine herpesvirus-1 infected cell protein 27. | bovine herpesvirus-1 infected cell protein 27 (bicp27) was detected predominantly in the nucleolus. the open reading frame of bicp27 was fused with the enhanced yellow fluorescent protein (eyfp) gene to investigate its subcellular localization in live cells and bicp27 was able to direct monomeric, dimeric or trimeric eyfp exclusively to the nucleolus. by constructing a series of deletion mutants, the putative nuclear localization signal (nls) and nucleolar localization signal (nols) were mapped ... | 2009 | 19682510 |
| exposure to pathogens and incidence of respiratory disease in young bulls on their arrival at fattening operations in france. | the incidence of clinical respiratory disease in 698 young beef bulls kept in 68 pens, and their exposure to respiratory pathogens after their arrival at 51 fattening operations in western france were assessed. antibodies against bovine respiratory syncytial virus (brsv), bovine herpesvirus type 1 (bhv-1), mannheimia haemolytica and mycoplasma bovis were measured by elisa. the incidence risk of respiratory disease was 18.5 per cent during the first six weeks. cases occurred in 37 of the 68 pens, ... | 2009 | 19684344 |
| cloning of bovine herpesvirus type 1 and type 5 as infectious bacterial artifical chromosomes. | bovine herpesviruses type 1 (bohv1) and type 5 (bohv5) are two closely related pathogens of cattle. the identity of the two viruses on the amino acid level averages 82%. despite their high antigenetic similarities the two pathogens induce distinctive clinical signs. bohv1 causes respiratory and genital tract infections while bohv5 leads to severe encephalitis in calves. | 2009 | 19828032 |
| inhibition of bovine herpesvirus multiplication in mdbk cells by small interfering rnas. | small interfering rna (sirna) mediated gene silencing is a promising approach in antiviral therapy. to investigate the antiviral effects of sirnas on bovine herpesvirus 1 (bohv-1) multiplication, we designed and in vitro synthesized two sirnas (sirna-1 and sirna-2) targeting the ul25 gene that is essential for bhv-1 multiplication. sirna-1 and sirna-2 inhibited the bohv-1 multiplication in mdbk cells to a different extent, namely by 11% and 40%, respectively, as demonstrated by virus titers (tci ... | 2009 | 19941403 |
| bovine respiratory disease research (1983-2009). | bovine respiratory disease (brd) research has provided significant understanding of the disease over the past 26 years. modern research tools that have been used include monoclonal antibodies, genomics, polymerase chain reaction, immunohistochemistry (ihc), dna vaccines and viral vectors coding for immunogens. emerging/reemerging viruses and new antigenic strains of viruses and bacteria have been identified. methods of detection and the role for cattle persistently infected bovine viral diarrhea ... | 2009 | 20003649 |
| host response to bovine respiratory pathogens. | bovine respiratory disease (brd) involves complex interactions amongst viral and bacterial pathogens that can lead to intense pulmonary inflammation (fibrinous pleuropneumonia). viral infection greatly increases the susceptibility of cattle to secondary infection of the lung with bacterial pathogens like mannheimia haemolytica and histophilus somni. the underlying reason for this viral/bacterial synergism, and the manner in which cattle respond to the virulence strategies of the bacterial pathog ... | 2009 | 20003650 |
| update on viral pathogenesis in brd. | many viruses, including bovine herpesvirus-1 (bhv-1), bovine respiratory syncytial virus (brsv), parainfluenzavirus-3 (pi3), bovine coronavirus, bovine viral diarrhea virus and bovine reovirus, have been etiologically associated with respiratory disease in cattle. this review focuses on the pathogenesis of bhv-1 and brsv, two very different agents that primarily cause disease in the upper and lower respiratory tract, respectively. | 2009 | 20003652 |
| a serological survey of antibodies against bovine herpesvirus-1 in yak (poephagus grunniens) in arunachal pradesh in india. | serum samples were collected from 254 yak (poephagus grunniens, presently bos grunniens) in different yak tracts of india. these samples were then screened by virus neutralisation test (vnt) and avidin-biotin enzyme-linked immunosorbent assay (ab-elisa) to study the seroprevalence of antibodies against bovine herpesvirus type 1 (bhv-1). the overall seroprevalence in yak was found to be 41% (105) by vnt and ab-elisa. the sex of the animal, whether it was on a farm or free-ranging and the location ... | 2009 | 20462162 |
| lactoferrin from bovine milk inhibits bovine herpesvirus 1 in cell culture but suppresses development of in vitro-produced bovine embryos. | bovine herpesvirus 1 (bohv-1) is widely distributed among cattle populations and has been associated with cells, fluids, and tissues collected from donor animals for use in reproductive technologies. the purpose of this study was to determine if lactoferrin would inhibit bohv-1 in cell culture and to evaluate if embryos could develop normally when cultured in vitro with lactoferrin. in experiment 1, lactoferrin (10 mg/ml) inhibited up to 25,000 plaque forming units (pfu)/ml of bohv-1 in madin da ... | 2009 | 18586420 |
| microarray-based detection of viruses causing vesicular or vesicular-like lesions in livestock animals. | definitive diagnosis of vesicular or vesicular-like lesions in livestock animals presents challenges both for veterinary clinicians and diagnostic laboratories. it is often impossible to diagnose the causative disease agent on a clinical basis alone and difficult to collect ample vesicular epithelium samples. due to restrictions of time and sample size, once laboratory tests have ruled out foot-and-mouth disease, vesicular stomatitis and swine vesicular disease a definitive diagnosis may remain ... | 2009 | 18621489 |
| bovine herpesvirus type 1 (bhv-1) and bovine viral diarrhoea virus (bvdv) infections in dairy herds: self clearance and the detection of seroconversions against a new atypical pestivirus. | the epidemiology of bovine herpesvirus type 1 (bhv-1) and bovine viral diarrhoea virus (bvdv) was studied in a population of small dairy herds that had not been vaccinated. bulk tank milk samples of 186 herds in thailand were collected four times between 2002 and 2004. serum samples from individual animals in 11 herds were also taken on three occasions. the prevalence of bhv-1 in the 186 herds was 61% in 2002, decreasing to 48% in 2004 and for bvdv was 91% in 2002, decreasing to 72% in 2004. a b ... | 2009 | 18760643 |
| comparison of the biobullet versus traditional pharmaceutical injection techniques on injection-site tissue damage and tenderness in beef subprimals. | the incidence and severity of injection-site lesions has decreased since the development of the beef quality assurance program. the objective was to evaluate the route of administration and the pharmaceutical product on the impact on tenderness, collagen concentration, and lesion occurrence in muscles of chucks and rounds. a total of 144 yearling steers (initial bw = 383 +/- 29.4 kg) were selected and transported to oklahoma state university. steers were blocked into 2 groups of 72 based on init ... | 2009 | 18849383 |
| evaluation of trypsin treatment on the inactivation of bovine herpesvirus type 1 on in vitro produced pre-implantation embryos. | the aim of this study was to evaluate the efficiency of trypsin treatment on the inactivation of bovine herpesvirus type 1 (bohv-1) on in vitro produced by fertilization and artificially infected bovine embryos. bovine embryos on day 7 were exposed with 10 microl of bohv-1, los angeles strain 10(7.5) tcid. these embryos and control embryos were divided in two groups: submitted to the sequential washes or to the trypsin treatment according to the international embryo transfer society (iets) guide ... | 2009 | 18992124 |
| multiple molecular regions confer intracellular localization of bovine toll-like receptor 8. | toll-like receptors (tlrs), a family of highly conserved germline-encoded pattern-recognition receptors (prrs), are essential for the host immune response. the cellular localization of tlr proteins determines the access to certain sources of ligands and thus the triggering of downstream cellular signaling. the tlr7/8/9 subfamily proteins are localized intracellularly but the molecular elements determining the cellular localization of these proteins are not fully understood. here we demonstrated ... | 2009 | 19019441 |
| antibody response against three widespread bovine viruses is not impaired in holstein cattle carrying bovine leukocyte antigen drb3.2 alleles associated with bovine leukemia virus resistance. | due to the wide dissemination of bovine leukemia virus (blv) infection among dairy cattle, control and eradication programs based on serological detection of infected cattle and subsequent culling face a major economic task. in argentina, genetic selection of cattle carrying alleles of the bovine leukocyte antigen (bola) drb3.2 gene associated with blv-infection resistance, like *0902, emerges as the best additional tool toward controlling virus spread. a potential risk in expanding or segregati ... | 2009 | 19109295 |
| bovine herpesvirus type 1 (bohv-1) anterograde neuronal transport from trigeminal ganglia to nose and eye requires glycoprotein e. | the requirement of bovine herpesvirus type 1 (bohv-1) envelope protein ge (us8 homolog) for establishment of latency and reactivation in trigeminal ganglia (tg) was examined. although bhv-1 ge-rescued and ge-deleted viruses were isolated from nasal or ocular swabs during primary infection, only the ge-rescued virus was isolated following dexamethasone-induced reactivation. furthermore, gc protein expression, which requires viral dna replication for its expression, was detected in tg of calves in ... | 2009 | 19115127 |
| the cellular transcription factor, ccaat enhancer-binding protein alpha (c/ebp-alpha), has the potential to activate the bovine herpesvirus 1 immediate-early transcription unit 1 promoter. | following acute infection, bovine herpesvirus-1 (bhv-1) establishes a lifelong latent infection in sensory neurons of trigeminal ganglia. bhv-1 periodically reactivates from latency and is shed as infectious virus. the latency-related (lr) gene is abundantly expressed in trigeminal ganglia of infected calves, and proteins encoded by the lr gene are necessary for reactivation from latency. we previously demonstrated that a novel lr protein interacts with a host transcription factor, ccaat enhance ... | 2009 | 19115128 |
| amended recombinant cells (arcs) expressing bovine ifn-gamma: an economical and highly effective adjuvant system. | interferon-gamma (ifn-gamma) has potent immune stimulatory activity, but purifying recombinant protein is expensive, soluble cytokine delivery is inefficient, and high doses of ifn-gamma cause adverse systemic effects. we've shown, however, that chemically fixed, amended recombinant pseudomonas fluorescens cells (arcs), expressing soluble bovine ifn-gamma (bgi/arcs), provide an effective production and delivery vehicle for highly active cytokine. in this report we investigate the immune enhancin ... | 2009 | 19150640 |
| coinfection with two closely related alphaherpesviruses results in a highly diversified recombination mosaic displaying negative genetic interference. | phylogenetic studies of the emergence and spread of natural recombinants in herpesviruses infecting humans and animals have been reported recently. however, despite an ever-increasing amount of evidence of recombination in herpesvirus history, the recombination process and the consequences on the genetic diversity of the progeny remain poorly characterized. we addressed this issue by using multiple single-nucleotide polymorphisms (snps) differentiating the two subtypes of an alphaherpesvirus, bo ... | 2009 | 19153224 |
| the infected cell protein 0 encoded by bovine herpesvirus 1 (bicp0) associates with interferon regulatory factor 7 and consequently inhibits beta interferon promoter activity. | the bicp0 protein encoded by bovine herpesvirus 1 stimulates productive infection and viral gene expression but inhibits interferon (ifn)-dependent transcription. bicp0 inhibits beta ifn (ifn-beta) promoter activity and induces degradation of ifn regulatory factor 3 (irf3). although bicp0 inhibits the trans-activation activity of irf7, irf7 protein levels are not reduced. in this study, we demonstrate that bicp0 is associated with irf7. furthermore, bicp0 inhibits the ability of irf7 to trans-ac ... | 2009 | 19176627 |
| a four year longitudinal sero-epidemiological study of bovine herpesvirus type-1 (bhv-1) in adult cattle in 107 unvaccinated herds in south west england. | bovine herpesvirus type-1 (bhv-1) is an important pathogen of cattle that presents with a variety of clinical signs, including the upper respiratory tract infection infectious bovine rhinotracheitis (ibr). a seroepidemiological study of bhv-1 antibodies was conducted in england from 2002 - 2004: 29,782 blood samples were taken from 15,736 cattle from 114 herds which were visited on up to three occasions. antibody concentration was measured using a commercial elisa. farm management information wa ... | 2009 | 19183476 |
| [examination of biocides for their effectiveness against animal viruses according to european union standards with emphasis on the selection of a suitable test virus]. | because of the changes to be expected in the methods for testing disinfectants deemed to be used in the veterinary field, candidate viral species were evaluated for their suitability as test virus. considered viral species included different non-enveloped viruses [bovine enterovirus type 1 (ecbo (enteric cytopathogenic bovine orphan) virus), mammalian reovirus type 1, feline calici virus (fcv), and bovine parvovirus (bpv)], as well as enveloped viruses, as equine arteritisvirus (eav), bovine her ... | 2009 | 19226936 |
| evaluation of three commercial bovine elisa kits for detection of antibodies against alphaherpesviruses in reindeer (rangifer tarandus tarandus). | the genus varicellovirus (family herpesviridae subfamily alphaherpesvirinae) includes a group of viruses genetically and antigenically related to bovine herpesvirus 1 (bohv-1) among which cervid herpesvirus 2 (cvhv-2) can be of importance in reindeer. these viruses are known to be responsible for different diseases in both wild and domestic animals. reindeer are a keystone in the indigenous saami culture and previous studies have reported the presence of antibodies against alphaherpesviruses in ... | 2009 | 19272136 |
| enhanced immune response and protection efficacy of a dna vaccine constructed by linkage of the mycobacterium tuberculosis ag85b-encoding gene with the bvp22-encoding gene. | plasmid dna vaccines have been widely explored for use in tuberculosis immunization but their immunogenicity needs improvement. in the present study, we incorporated the bovine herpesvirus 1 vp22 (bvp22)-encoding gene, which encodes a protein that demonstrates a capability for disseminating the expressed antigen to neighbouring cells, into a dna vector in which it was fused to the ag85b-encoding gene of mycobacterium tuberculosis (mtb), and investigated whether this linkage could enhance immune ... | 2009 | 19273642 |
| cervid herpesvirus 2 causes respiratory and fetal infections in semidomesticated reindeer. | members of the viral subfamily alphaherpesvirinae establish latency from which they can be reactivated. bovine herpesvirus 1 causes infectious bovine rhinotracheitis and infectious pustular vulvovaginitis in cattle, as well as abortion and weak calves. serological evidence of alphaherpesvirus infection has been reported for wild and semidomesticated reindeer (rangifer tarandus tarandus) in norway. to address the possibility that reindeer alphaherpesvirus (cervid herpesvirus 2 [cvhv-2]) infection ... | 2009 | 19279181 |
| granular vulvovaginitis syndrome in nelore pubertal and post pubertal replacement heifers under tropical conditions: role of mycoplasma spp., ureaplasma diversum and bhv-1. | in order to determine the role of mycoplasma spp, ureaplasma diversum and bhv-1 as causal agents of granular vulvovaginitis syndrome in nelore heifers raised under tropical conditions and based on the hypothesis that stressful conditions during puberty or breeding season would be a determinant factor for the infection, 340 heifers not vaccinated against bhv-1 were divided in post-pubertal, in the beginning of the first breeding season, and pubertal heifers. the vaginal lesion score (vls) grade 1 ... | 2009 | 19319656 |
| construction of a recombinant bhv-1 expressing the vp1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model. | foot-and-mouth disease (fmd) and infectious bovine rhinotracheitis (ibr) are two important infectious diseases of cattle. using bovine herpesvirus type 1 (bhv-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. in this study, a recombinant bhv-1 was constructed by inserting the synthetic fmdv (o/china/99) vp1 gene in the the ge locus of bhv-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phie cmv) and bovine ... | 2009 | 19343503 |
| modified bovine herpesvirus 1 for protein secretion. | the traditional way to utilize bovine herpesvirus 1 (bhv-1) and many other herpesviruses as vectors for synthesis of heterologous proteins like reporter proteins, antigens, or immunomodulatory active molecules was (and still is) the expression of the protein of interest from an entire gene consisting of promoter, 5'- and 3'-noncoding regions, the open reading frame (orf), and a signal sequence for polyadenylation. this approach is doubtlessly appropriate especially in cases when expression of la ... | 2009 | 19378128 |
| bovine herpesvirus type 1 infection of bovine bronchial epithelial cells increases neutrophil adhesion and activation. | respiratory infection of cattle with bovine herpesvirus type 1 (bhv-1) predisposes cattle to secondary pneumonia with mannheimia haemolytica as part of the bovine respiratory disease complex (brd). one cell type that has received limited investigation for its role in the inflammation that accompanies brd is the respiratory epithelial cell. in the present study we investigated mechanisms by which bhv-1 infection of respiratory epithelial cells contributes to the recruitment and activation of bovi ... | 2009 | 19406483 |
| the essential and non-essential genes of bovine herpesvirus 1. | bovine herpesvirus 1 (bohv-1) is an economically important pathogen of cattle associated with respiratory and reproductive disease. to further develop bohv-1 as a vaccine vector, a study was conducted to identify the essential and non-essential genes required for in vitro viability. random-insertion mutagenesis utilizing a tn5 transposition system and targeted gene deletion were employed to construct gene disruption and gene deletion libraries, respectively, of an infectious clone of bohv-1. tra ... | 2008 | 18931083 |
| prevalence of infectious agents in free-ranging white-tailed deer in northeastern mexico. | the objectives of this study were to determine the prevalence of antibodies against brucellosis, leptospirosis, infectious bovine rhinotracheitis virus, and bovine viral diarrhea virus (bvdv) in white-tailed deer (odocoileus virginianus) in northeastern mexico. deer (n=521) were captured from helicopter using a netgun on 15 ranches covering 62,114 ha in the states of coahuila, nuevo leon, and tamaulipas during spring 2004. the prevalence of antibodies against leptospira, infectious bovine rhinot ... | 2008 | 18957659 |
| the varicellovirus ul49.5 protein blocks the transporter associated with antigen processing (tap) by inhibiting essential conformational transitions in the 6+6 transmembrane tap core complex. | tap translocates virus-derived peptides from the cytosol into the endoplasmic reticulum, where the peptides are loaded onto mhc class i molecules. this process is crucial for the detection of virus-infected cells by ctl that recognize the mhc class i-peptide complexes at the cell surface. the varicellovirus bovine herpesvirus 1 encodes a protein, ul49.5, that acts as a potent inhibitor of tap. ul49.5 acts in two ways, as follows: 1) by blocking conformational changes of tap required for the tran ... | 2008 | 18802093 |
| double immunization strategy with a bohv-4-vectorialized secreted chimeric peptide bvdv-e2/bohv-1-gd. | a bovine herpesvirus 4 was isolated from the milk cell fraction of a healthy cow and his full genome cloned as a bacterial artificial chromosome. so cloned viral genome was used as a vector platform to deliver in vitro and in vivo an optimized secreted chimeric peptide obtained by the fusion of the bovine viral diarrhoea virus glycoprotein e2 ectodomain with the bovine herpesvirus 1 glycoprotein d ectodomain. recombinant virus infected cells robustly expressed and secreted the chimeric peptide i ... | 2008 | 18812200 |
| serosurveillance of viral diseases in korean native goats (capra hircus). | a total of 804 goat sera were collected from 144 goat farms in five regions of south korea during a period between 2005 and 2006 and screened for the antibodies of viral pathogens in ruminants. the individual seropositive rates for each virus were 13.7% (110/804) for bovine herpesvirus-1 (bhv-1), 9.5% (76/804) for bovine parainfluenza type-3 virus (pi-3v), 5.5% (44/804) for akabane virus (akav), 13.3% (107/804) for aino virus (ainv), 2.0% (16/804) for chuzan virus (chuv) and 1.0% (8/804) for bov ... | 2008 | 18840974 |
| the zinc ring finger of bovine herpesvirus 1-encoded bicp0 protein is crucial for viral replication and virulence. | bovine herpesvirus 1 (bhv-1) infected cell protein 0 (bicp0) stimulates productive infection, in part by activating viral gene expression. the c(3)hc(4) zinc ring finger of bicp0 is crucial for activating viral transcription and productive infection. in this study, we used a bacterial artificial chromosome containing a wild-type (wt) virulent bhv-1 strain to generate a single amino acid mutation in the c(3)hc(4) zinc ring finger of bicp0. this virus (the 51g mutant) contains a cysteine-to-glycin ... | 2008 | 18842710 |
| 2,3,7,8-tetrachlorodibenzo-p-dioxin regulates bovine herpesvirus type 1 induced apoptosis by modulating bcl-2 family members. | exposure to environmental contaminants, like 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd), leads to an increased susceptibility to infectious agents. infection of bovine cells (mdbk) with bovine herpesvirus 1 (bhv-1) anticipates virus-induced apoptosis, suggesting an involvement of tcdd in virus infection. herein we analyzed the effects of tcdd on apoptotic pathway in mdbk cells infected with bhv-1. after 12 h of infection, tcdd induced a significant increase in apoptotic cells. tcdd caused a dose ... | 2008 | 18696228 |
| open reading frame 2, encoded by the latency-related gene of bovine herpesvirus 1, has antiapoptotic activity in transiently transfected neuroblastoma cells. | the latency-related (lr) rna encoded by bovine herpesvirus 1 is abundantly expressed in the trigeminal ganglia of latently infected calves. expression of lr proteins is necessary for reactivation from latency and the protection of infected neurons from apoptosis. in this study, we demonstrated that an lr-encoded protein, open reading frame 2 (orf-2), or orf-2 fusion proteins encoded by alternatively spliced lr transcripts inhibit cold shock or fas ligand-induced apoptosis in mouse neuroblastoma ... | 2008 | 18715935 |
| the ul49 gene product of bohv-1: a major factor in efficient cell-to-cell spread. | the role of the ul49 gene product, vp22, of bovine herpesvirus type 1 (bohv-1) in virus replication was characterized with respect to a putative functional interaction of vp22 with the viral glycoprotein e (ge) during bohv-1 cell-to-cell spread. deletion of the open reading frames of ul49 and/or ge from an infectious bohv-1 bacterial artificial chromosome clone did not severely impair the production of viral progeny in single-step growth experiments. however, plaque sizes induced by a vp22-negat ... | 2008 | 18753236 |
| associations between health and productivity in cow-calf beef herds and persistent infection with bovine viral diarrhea virus, antibodies against bovine viral diarrhea virus, or antibodies against infectious bovine rhinotracheitis virus in calves. | to measure associations between health and productivity in cow-calf beef herds and persistent infection with bovine viral diarrhea virus (bvdv), antibodies against bvdv, or antibodies against infectious bovine rhinotracheitis (ibr) virus in calves. | 2008 | 18593246 |
| effect of age at the time of vaccination on antibody titers and feedlot performance in beef calves. | to compare antibody responses, feedlot morbidity and mortality rates, feedlot performance, and carcass value for calves vaccinated with 1 of 2 vaccination strategies and for unvaccinated control calves. | 2008 | 18593324 |
| eicosanoids mediate melanotic nodulation reactions to viral infection in larvae of the parasitic wasp, pimpla turionellae. | nodulation is the quantitatively predominant insect cellular immune function activated in response to bacterial, fungal and some viral infections. we posed the hypothesis that parasitoid insects express melanotic nodulation reactions to viral challenge and that eicosanoids mediate these reactions. treating fifth-instar larvae of the ichneumonid endoparasitoid pimpla turionellae with bovine herpes simplex virus-1 (bhsv-1) induced nodulation reactions in a challenge dose-dependent manner. experime ... | 2008 | 17904576 |
| expression of iron-related proteins during infection by bovine herpes virus type-1. | bovine herpesvirus 1 (bhv-1), a dsdna animal virus, is an economically important pathogen of cattle and the aetiological agent of many types of disease. the efficient replication of a dna virus is strictly dependent on iron since this metal plays a crucial role in the catalytic center of viral ribonucleotide reductase. consequently, iron metabolism is an important area for virus/host interaction and a large body of evidence suggests that viral infection is potentially influenced by the iron stat ... | 2008 | 17990282 |
| serological evidence of caprine herpesvirus 1 infection in mediterranean france. | caprine herpesvirus 1 (cphv-1) is responsible of a systemic disease in kids and genital diseases inducing abortions in adult goats. in europe, cphv-1 is widespread in mediterranean countries such as greece, italy and spain. as france is geographically close to these countries, a survey was conducted to investigate the presence of cphv-1 in goats in a mediterranean department (corse-du-sud) and in continental departments (dordogne and vendée) of this country. taking into account the close antigen ... | 2008 | 18031957 |
| the presence of a deletion sequence in the bhv-1 ul49 homolog in a live attenuated vaccine for infectious bovine rhinotracheitis (ibr). | the difference between the attenuated live vaccine strain 758-43 and its parent virulent strain 758 was investigated genetically. these viruses were propagated in madin-darby bovine kidney cells, and viral dna was obtained from the culture supernatants of the infected cells. based on a previous report, a large deleted region would seem to exist in the hind iii j fragment located between nucleotide numbers 2439 and 11,270. three pairs of primers were designed based on the complete bhv-1 dna seque ... | 2008 | 18164789 |
| respiratory disease associated with bovine coronavirus infection in cattle herds in southern italy. | four outbreaks of bovine respiratory disease (brd) associated with bovine coronavirus (bcov) infection in italian cattle herds were reported. in 3 outbreaks, brd was observed only in 2-3-month-old feedlot calves, whereas in the remaining outbreak, lactating cows, heifers, and calves were simultaneously affected. by using reverse transcription polymerase chain reaction (rt-pcr), bcov rna was detected in all outbreaks without evidence of concurrent viral pathogens (i.e., bovine respiratory syncyti ... | 2008 | 18182504 |
| humoral and t cell-mediated immune responses to bivalent killed bovine viral diarrhea virus vaccine in beef cattle. | the objective of this research project was to evaluate the antibody and cell-mediated immune responses to a multivalent vaccine containing killed bovine viral diarrhea virus (bvdv) types 1 and 2. twenty castrated male crossbred beef cattle (350-420kg body weight) seronegative to bvdv were randomly divided into two groups of 10 each. group 1 served as negative mock-vaccinated control. group 2 was vaccinated subcutaneously twice, 3 weeks apart, with modified live bovine herpesvirus 1, parainfluenz ... | 2008 | 18190971 |
| effects of on-arrival versus delayed modified live virus vaccination on health, performance, and serum infectious bovine rhinotracheitis titers of newly received beef calves. | stress commonly associated with weaning, marketing, and shipment of feeder cattle can temporarily compromise immune function, thereby reducing the effective response to vaccination intended to control bovine respiratory disease (brd). two vaccination timing treatments were used to evaluate the effect of timing of a multivalent modified live virus (mlv) brd vaccine on health, performance, and infectious bovine rhinotracheitis (ibr) antibody titers of newly received stocker cattle. crossbred bull ... | 2008 | 18192559 |
| bovine herpesvirus type 1 induces cell death by a cell-type-dependent fashion. | bovine herpesvirus 1 (bhv-1), a member of the alpha-herpesvirinae sub-family, causes significant losses to the cattle industry. bhv-1 establishes latency in trigeminal ganglionic sensory neurons, but periodically reactivates from latency. previous studies suggested that infection with bhv-1-induced novel morphological changes in rabbit skin (rs) cells versus bovine kidney cells (mdbk). consequently, we hypothesized that viral infection led to a novel form of cell death in rs cells compared to md ... | 2008 | 18222625 |
| efficacy of a recombinant trypsin product against bovine herpesvirus 1 associated with in vivo- and in vitro-derived bovine embryos. | although porcine-origin trypsin will effectively remove bovine herpesvirus 1 (bhv-1) associated with in vivo-derived embryos, tryple, a recombinant trypsin-like protease, has not been evaluated. in experiment 1, 17 groups of 10 in vivo-derived embryos were exposed to bhv-1, treated with tryple express or tryple select (10x concentration) for varying intervals, and assayed as 2 groups of 5 embryos. tryple select treatment for 5 and 10 min (two and seven groups of five embryos, respectively) effec ... | 2008 | 18242686 |
| validation of betapropiolactone (bpl) as an inactivant for infectious bovine rhinotracheitis (ibr) virus. | infectious bovine rhinotracheitis (ibr) virus causes vulvovaginitis, abortion and respiratory disease in cows and heifers. betapropiolactone (bpl) is a disinfectant, effective against bacteria, fungi and viruses. it is also used to prepare inactivated vaccines because it destroys the nucleic acid core of viruses but does not damage the capsid. for the validation of bpl when used as an inactivant, it is more important to assure the quality of inactivating agent and the validity of the inactivatio ... | 2008 | 18262212 |