Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cerebeller afferents from neurons in motor nuclei of cranial nerves demonstrated by retrograde axonal transport of horseradish peroxidase. | 1977 | 72590 | |
| somatopetal transport of horseradish peroxidase (hrp) in the peripheral and central branches of dorsal root ganglion cells. | the axonal transport of hrp in both the peripheral and central branches of dorsal root ganglion cells was studied in rats. for studying axonal transport in the peripheral branch hrp as a dry substance was applied to the peroneal nerve injured either by teasing, by cutting or crushing. after a short survival time (22 h) mainly small spinal ganglion cells of the corresponding segments were labelled, while after a prolonged survival time (70 h) mainly large cells were labelled. these labelling diff ... | 1977 | 72611 |
| afferents to the entorhinal cortex of the rat studied by the method of retrograde transport of horseradish peroxidase. | 1977 | 72679 | |
| effect of cyanide on nadph oxidation by granules from human polymorphonuclear leukocytes. | cyanide has been shown to stimulate both oxygen uptake and hexose monophosphate shunt activity in phagocytizing human polymorphonuclear leukocytes. it also stimulates the oxidation of nadph by a particulate fraction derived from phagocytizing cells. this stimulation of nadph oxidase is not observed in the presence of exogenous mn2+. studies with purified enzymes have shown that cn- also stimulates nadph oxidation by horseradish peroxidase or lactoperoxidase, suggesting that the respiratory burst ... | 1977 | 13879 |
| purification of bovine thyroid peroxidase. | trypsin-solubilized peroxidase activity from beef subcellular particles was resolved by deae-cellulose chromatography into 5 fractions, which contained enzymatically active components that ranged in molecular size from 73,000 to 340,000 daltons. the most active fraction (mol wt, 92,000 by gel filtration) was further purified (59,000-fold overall) by chromatography on hydroxylapatite. this highly purified peroxidase preparation had an absorbance purity ratio (a410:a280) of 0.55 and oxidized iodid ... | 1977 | 15822 |
| the role of cytochrome p-450 in the hydroperoxide-catalyzed oxidation of alcohols by rat-liver microsomes. | the organic hydroperoxide cumene hydroperoxide is capable of oxidizing ethanol to acetaldehyde in the presence of either catalase, purified cytochrome p-450 or rat liver microsomes. other hemoproteins like horseradish peroxidase, cytochrome c or hemoglobin were ineffective. in addition to ethanol, higher alcohols like 1-propanol, 1-butanol and 1-pentanol are also oxidized to their corresponding aldehydes to a lesser extent. other organic hydroxyperoxides will replace cumene hydroperoxide in oxid ... | 1977 | 20305 |
| adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin a, wheat germ agglutinin and goat anti-human immunoglobulin g on cell surfaces at the electron microscopic level: a new method, theory and application. | a method is described for the adsorption of selected macromolecules to colloidal gold which is then used as an electron dense marker for the indirect detection of specific cell surface molecules. membrane bound concanavalin a, which binds specific sugars on horseradish peroxidase, and wheat germ agglutinin, which binds specific sugars on ovomucoid are detected indirectly with gold labeled horseradish peroxidase and ovomucoid, respectively. goat anti-human igm on blood lymphocytes is detected wit ... | 1977 | 21217 |
| efferent connections of the septal area in the rat: an analysis utilizing retrograde and anterograde transport methods. | experiments were performed by either retrograde (horseradish peroxidase histochemistry) or anterograde ([3h]leucine radioautography) transport methods to determine the efferent connections of the septum in the rat. it was observed that the dorsal septum projects to the lateral preoptic area, lateral hypothalamus, periventricular hypothalamus and midline thalamus. fibers from the ventral half of the septum project topographically to the hippocampal formation, thalamus, hypothalamus and midbrain. ... | 1977 | 63306 |
| anatomical and functional aspects of the septo-hippocampal projections. | the origins, distribution, and cellular targets of the septo-hippocampal projections are reviewed. it appears that the distribution of acetylcholinesterase-positive neurons in the medial septum and diagonal bands and those cells labelled after injections of horseradish peroxidase into the hippocampus coincide; however, the possibility of a non-acetylcholinesterase septal projection remains. good agreement is found between the distribution of hippocampal acetylcholinesterase and the patterning of ... | 1977 | 83225 |
| [ultrastructural demonstration of the phagocytic activity of periodontal ligament fibroblasts in the rat using enzymatic tracers]. | the ultrastructural study of non-decalcified periodontal ligament of rats, labeled with horseradish peroxidase used as an enzymatic tracer for the mechanism of endocytosis, shows that there exists in the fibroblast, on the one hand, an endogeneous peroxidase revealed by the preparation and characteristic of certain cellular organelles: mitochondria, free ribosomes, ribosomes of the granular endoplasmic reticulum; and on the other hand an exogeneous peroxidase which demonstrates the ability of fi ... | 1977 | 122704 |
| the ultrastructural histochemistry of the basement membranes of the exocrine pancreas. | the basement membranes in the exocrine pancreas were examined by routine electron microscopy, by fixation for electron microscopy with a glutaraldehyde-tannic acid solution and by staining for ultrastructural demonstration of mucosubstances with the dialyzed iron, high iron diamine, ruthenium red and concanavalin a-horseradish peroxidase procedures. the basement membranes are considered from morphologic and histochemical observations to consist of an inner lamina lucida, an intermediate lamina ... | 1977 | 143223 |
| investigation of the endothelial cell changes in the brain of shr using horseradish peroxidase technique and enzyme histochemistry [proceedings]. | 1977 | 143545 | |
| hydrogen peroxide production and killing of staphylococcus aureus by human polymorphonuclear leukocytes. | the effects of bacterial neuraminidase on production of hydrogen peroxide (h2o2) and killing of staphylococcus aureus by human polymorphonuclear leukocytes (pmn) were studied. the concentration of h2o2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. the results indicated that desialylation of human pmn inhibited the stimulation of h2o2 production during phagocytosis. it also markedly impaired the killing of s. aureus. impaired killing of s. ... | 1977 | 189859 |
| relay cell classes in the lateral geniculate nucleus of the cat and the effects of visual deprivation. | this study presents evidence that the x- and y-cells described physiologically in the a laminae of the cat's dorsal lateral geniculate nucleus (lgn) are two morphologically distinct cell types recognizable in golgi preparations. it is shown firstly that the three cell types seen in golgi preparations of the a laminae (large and medium-sized principal cells and small interneurons-types 1,2 and 3 in the classification of guillery, '66) may be identified in 1-mum epon sections of osmicated material ... | 1977 | 190274 |
| tissue-specific antibodies against human lung and breast carcinoma dehistonized chromatins. | tissue-specific antisera against human lung and breast carcinoma dehistonized chromatins were obtained. the specificity of these antisera was determined by complement fixation. in the presence of antiserum against human lung carcinoma, only chromatins from lung carcinoma fixed complement significantly, whereas chromatins isolated from human breast carcinoma, hela cells, normal lung tissue, breast tissue, or term placenta were negative (i.e., inactive). in a similar assay with the use of antiseru ... | 1977 | 195066 |
| solid-phase enzyme immunoassay for immunoglobulin m antibodies to cytomegalovirus. | a sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin m antibodies to cytomegalovirus is described. the results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei ... | 1977 | 195977 |
| protein a-peroxidase: a valluable tool for the localization of antigens. | protein a of staphylococcus aureus has been conjugated to horseradish peroxidase and used in an indirect immunolabeling technique to visualize membrane and viral antigens. the same protein a-peroxidase conjugate was used with antisera from five different species. using this indirect test, membrane markers for t and b lymphocytes were labeled with a greater specificity than when peroxidase conjugated anti-immunoglobulin was used in the second step. viral antigens on cells infected with measles, v ... | 1977 | 199666 |
| the functional capacity of guinea pig megakaryocytes. ii. the uptake of particles and macromolecules and the effect of rabbit antiguinea pig platelet antiserum. | the capacity of megakaryocytes to take up particles and macromolecules was tested by exposing them to homologous erythrocytes, latex particles, micrococcus lysodeikticus, and horseradish peroxidase. uptake of particles 1.1 to 7 mum. in size was extensive under the experimental conditions employed. after uptake of each of the three particles the addition of tannic acid and calcium to the fixative produced electron-dense deposits within the vacuole surrounding the particle; therefore, the vacuole ... | 1977 | 320386 |
| an ultrastructure study on antibody production of the lymph nodes of rats with special reference to the role of germinal centers. | antibody-containing cells in lymph nodes, expecially in germinal centers, and in thoracic duct of rats sensitized by injection of horseradish peroxidase were studied with the immunoperoxidase method. in the primary response the large germinal center cells began to produce antibody on the 9th day and predominated thereafter in germinal centers ,added by intercellular antibody deposition in its late stage. the "ordinary" medium-sized germinal center cells were a minor component. in the secondary r ... | 1977 | 322442 |
| intimal healing. the pattern of reendothelialization and intimal thickening. | studies were undertaken to investigate further the basis of intimal proliferation and the identity of the surface lining cell in rabbit aortas subjected to extensive deendothelialization. endothelial cells were selectively removed by passage of an inflated balloon catheter through the arterial lumen. the healing response was evaluated at intervals up to 36 weeks by several techniques: 1) permeability to evans blue, 2) reappearance of endothelial cells as indicated by the specific marker, adsorbe ... | 1977 | 322503 |
| solid-phase enzyme immunoassay for hepatitis b surface antigen. | a solid-phase enzyme immunoassay is described for measuring hepatitis b surface antigen in human serum or plasma. immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. in the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specifici ... | 1977 | 322892 |
| ultrastructural immunocytochemical localisation of lysozyme in human bronchial glands. | an immunocytochemical unlabelled antibody method using rabbit antihuman lysozyme, antirabbit immunoglobulin, and soluble rabbit antihorseradish peroxidase/horseradish peroxidase complexes was used to study the fine structural distribution of lysozyme in human bronchial glands. none was identified in mucous cells but there was heavy staining of the serous cell granules. the serous cell granules were not stained uniformly, suggesting the presence of other secretory products but lysozyme secretion ... | 1977 | 325682 |
| the localization of proteoglycans and glycoproteins in the hyaline cartilage. | antibodies to proteoglycan (pg) and glycoprotein of bovine nasal cartilage were conjugated with fluorescein isothiocyanate and with horseradish peroxidase. hyaluronidase digestion of cartilage tissue-specimens increased the intensity of immune reactions; pronase digestion or extraction with 4 m guanidinium chloride abolished the staining. in the intercellular matrix fine filaments beaded with small granules were seen forming an irregular network. the interstices of the network are filled with co ... | 1977 | 332674 |
| immune complex receptors on cell surfaces. iii. topography of macrophage receptors demonstrated by new scanning electron microscopic peroxidase marker. | receptors for immune complexes have been localized on rabbit alveolar macrophages with scanning electron microscopy by exposing the cells first to a soluble immune complex composed of horseradish peroxidase and antibody to horseradish peroxidase, and then incubating with a benzidine-containing substrate that yields crystalline reaction product. receptors were visualized by this means as sites of attachment of laminated slender crystals that were easily distinguished from macrophage surface struc ... | 1977 | 333019 |
| evaluation of some of the parameters of the enzyme-linked immunospecific assay. | in an attempt to standardize the procedure for the enzyme-linked immunospecific assay, several parameters were examined. it was determined that horseradish peroxidase was the enzyme of choice and that several brands of polystyrene tubes and plates could be used. the test was considerably shortened by omitting long incubation steps previously used for eliminating background fixation of conjugate. the reproducibility of the procedure proved to be excellent, but reactivity curves indicated that use ... | 1977 | 333043 |
| a specific enzymeimmunoassay for progesterone in human plasma. | an enzymeimmunoassay for plasma progesterone was established using progesterone covalently linked to the enzyme, horseradish peroxidase, as the 'label'. separation of free and bound steroid was effected by sepharose-coupled antiprogesterone-11alpha-hemisuccinyl bovine serum albumin antiserum (sepharose-antisera). the enzymeimmunoassay satisfied the normal criteria of specificity, precision and accuracy. comparison of assay results obtained by radioimmunoassay (with and without thin-layer chromat ... | 1977 | 333666 |
| the blood-brain barrier to horseradish peroxidase under normal and experimental conditions. | this review paper deals with the transport of the protein tracer horseradish peroxidase across cerebral vessels under normal and various experimental conditions. electronmicroscopical investigations have revealed that, under normal conditions, a minor vesicular transfer of intravenously injected peroxidase occurs across the endothelium in segments of arterioles, capillaries and venules, especially in arterioles with a diameter about 15-30 mu. this normally occurring vesicular transport is suscep ... | 1977 | 333857 |
| development of an enzyme-linked receptor assay (era) for hcg. | an enzyme-linked receptor assay (era) for hcg was developed using horseradish peroxidase. the 1,500 g pellets, interstitial cell fraction, and solubilized homogenate from the rat testis linked to tanned sheep red blood cells (srbc) were used as the binding fractions for hcg. in these era systems, the limit of detection for hcg was 30 iu/l (3-12 miu/tube), which was almost equal to that of radio receptor assay (rra). the era using srbc linked solubilized receptor fraction showed the most satisfac ... | 1977 | 334520 |
| uptake of horseradish peroxidase by geniculo-cortical axons in the golden hamster: analysis by computer reconstruction. | micro-injections of horseradish peroxidase (hrp) were made into the visual cortex of the golden hamster. the "projection lines" of labelled neurons in the dorsal lateral geniculate nucleus (lgnd) were three-dimensionally reconstructed, using a computer graphics technique. the lines run rostrally and medially from their origins at the lateral surface of the nucleus. using an anatomically determined retinotopic map of the lgnd, the positions of all labelled cells near the lateral surface were conv ... | 1977 | 334561 |
| studies on translocation of immunoglobulins across intestinal epithelium. iv. evidence for binding of iga and igm to secretory component in intestinal epithelium. | we conducted studies concerning the issue of whether secretory component (sc) is a specific receptor on intestinal epithelial cells for iga and igm. initially, frozen sections of human intestinal mucosa were incubated with dimeric monoclonal human iga, conjugated to horseradish peroxidase; adjacent sections were reacted with peroxidase-conjugated antibodies to sc. the conjugated iga and anti-sc bound to similar sites in the epithelium, that is to basolateral margins and supranuclear cytoplasm of ... | 1977 | 334623 |
| absorption of horseradish peroxidase by neonatal pig intestinal epithelium: effect of escherichia coli (055b5) on absorption. | the pathway of macromolecular transport through the neonatal pig small intestinal epithelium was examined, utilizing the cytochemical marker horseradish peroxidase (hrp). the marker was found adsorbed to the apical microvillous of the enterocytes, within apical tubules, and cytoplasmic vacuoles. extracellular absorbed marker assumed a spherical appearance within the subepithelial spaces but was dispersed within the capillary lumens. uptake of hrp into the enterocyte occurred in 48-hour old neona ... | 1977 | 335926 |
| enzyme immunoassay for hepatitis b and its comparison to other methods. | a solid-phase enzyme immunoassay for the qualitative detection of hepatitis b surface antigenemia is presented and the results compared to the counter-immunoelectrophoresis and radioimmunoassay methods for detection of the same antigen. the enzyme-antibody conjugate was prepared from horseradish peroxidase coupled with antibodies to three sub-types of hepatitis b virus. polystyrene plastic tubes were used as the solid-phase support. the results of the enzyme immunoassay compare favorably with ra ... | 1977 | 336244 |
| identification of synaptic acetylcholine receptor sites in retina with peroxidase-labeled alpha-bungarotoxin. | an alpha-bungarotoxin-horseradish peroxidase conjugate, which binds specificity to nicotinic acetylcholine receptors, was synthesized. this conjugate was bound by 5-7% of the synapses in the inner plexiform layer of the chicken retina. bipolar ribbon synapses as well as amacrine synapses bound the conjugate. | 1977 | 269390 |
| effect of photocoagulation on the barrier function of the pigment epithelium. ii. a study by electron microscopy. | the healing of retinal photocoagulation burns and their permeability to colloidal carbon and horseradish peroxidase was studied by electron microscopy. recent burns showed disruption of intercellular junctions in the pigment epithelium. by 2 days there was infiltration of the burn by proliferating pigment epithelial cells, and by 7 days the burn was composed of a mass of interconnected cells of pigment epithelial and müller cell origin. fresh photocoagulation burns result in complete disruption ... | 1977 | 281795 |
| ultrastructural observations of horseradish peroxidase in synovial lining cells of the rats' temporomandibular joint. | 1977 | 274462 | |
| induction of pinocytosis in rat hepatocytes by partial hepatectomy. | rat hepatocytes, normally not highly pinocytic cells, becomes so after partial hepatectomy when about two-thirds of the liver is removed. droplets, up to 20 mum in diameter, develop, initially by addition to smaller pinocytic structures and later by fusion with lysosomes. the droplets contain a material with an electron microscope periodicity characteristic of fibrin; they are periodic acid schiff-positive as is plasma. it is therefore reasonable to consider plasma glycoproteins to be major comp ... | 1977 | 557041 |
| [regulation of antibody secretion: observation made on cultured lymphoid cells from immune rabbit lymph nodes]. | rabbits were immunized with soluble and particulate antigens (horseradish peroxidase or srbc) in complete freund's adjuvant. four to twelve weeks after footpad immunization, popliteal lymph nodes were removed and the lymphoid cells were cultured with autologous serum for 5 to 7 days. using local hemolysis assay, we observed a recrudescence of antibody secreting cells when the autologous serum present in the medium had previously been freed of antibody by specific adsorption. this phenomenon was ... | 1977 | 557948 |
| experimental spinal cord compression by epidural neoplasm. | we have developed an experimental model of spinal cord compression in rats. tumor injected anterior to the t-12 vertebral body grows through the intervertebral foramina to compress the cord and produces paraplegia in 3 to 4 weeks. evidence for vasogenic edema in spinal cord compressed by tumor includes increased water content, leakage of horseradish peroxidase into gray matter, and histologic evidence of edema. the vascular supply to the cord overlying the tumor appears to be compromised. both s ... | 1977 | 558545 |
| septal afferents to the arcuate-median eminence region in the guinea pig: correlative electrophysiological and horseradish peroxidase studies. | 1977 | 562702 | |
| probing axial ligands in ferric haemoproteins: an esr study of myoglobin and horseradish peroxidase in h217o. | 1977 | 202277 | |
| the visual cortex of the opossum: the retrograde transport of horseradish peroxidase to the lateral geniculate and lateral posterior nuclei. | the visual cortex of opossum was studied by injecting horseradish peroxidase into the cortex and identifying labeled neurons in the thalamus. the results show that the lateral geniculate nucleus projects to area 17 in a topographical manner: the rostral lateral geniculate is represented in caudal striate cortex, and the dorsal extremity of the lateral geniculate, which probably corresponds to the zero vertical meridian, is represented along the border of area 18. small injections in area 17 prod ... | 1977 | 73403 |
| membrane alterations during cornification of mammalian squamous epithelia: a freeze-fracture, tracer, and thin-section study. | tight junctions (zonulae occludentes) create a pericellular barrier to the diffusion of large molecules in non-keratinizing mammalian epithelia. however, in cornifying epithelia such as the epidermis, the importance of tight-junctional elements versus secreted intercellular lipid for barrier function is uncertain. in an attempt to resolve this question, we compared membrane structure in the stratum granulosum and stratum corneum of epidermis, esophagus, and vagina of newborn and adult humans and ... | 1977 | 339780 |
| circular dichroism studies on native and artificial horseradish peroxidases. | 1977 | 914868 | |
| effect of indole-3-acetic acid on the kinetics of horseradish peroxidase catalyzed scopoletin oxidation. | the kinetics of horseradish peroxidase catalyzed of scopoletin oxidation were observed to be sigmoidal. the apparent kms for scopoletin is 0.9 mm. inhibition of scopoletin oxidation by indole-3-acetic acid (iaa) appears to be non competitive. non competitive inhibition by iaa suggests a more significant role of the peroxidase protein matrix in regulating the activity of the heme moiety. | 1977 | 923704 |
| [silymarin--an inhibitor of horseradish peroxidase (author's transl)]. | silybin and silychristin non-competitively inhibit the horseradish peroxidase in vitro. silydianin on the other hand is a competitive inhibitor of the enzyme. | 1977 | 923693 |
| nuclear magnetic resonance studies of hemoproteins. acid-alkaline transition, ligand binding characteristics, and structure of the heme environments in horseradish peroxidase. | 1977 | 20945 | |
| descending input from the pneumotaxic system to the lateral respiratory nucleus of the medulla. an anatomical study with the horseradish peroxidase technique. | 1977 | 19605040 | |
| high resolution of peroxidase-indoleacetic acid oxidase isoenzymes from horseradish by isoelectric focusing. | several improved techniques for isoelectric focusing of isoenzymes in polyacrylamide gel slabs were developed. using these techniques, three commercial sources of horseradish peroxidase were each examined with three commercial sources of carrier ampholytes to determine their respective isoenzyme profiles.a much higher degree of isoenzyme resolution was obtained than reported previously. a composite of all of the various tests gave a total of 42 peroxidase isoenzymes. commercial sources of horser ... | 1977 | 16660185 |
| a sensitive histochemical method for light-and electron-microscopic demonstration of horseradish peroxidase. | 1977 | 915248 | |
| a new specific, sensitive and non-carcinogenic reagent for the demonstration of horseradish peroxidase. | 1977 | 72746 | |
| fine structural and ultracytochemical studies on the lymphocytes in three types of genetic mucopolysaccharidoses. | lymphocytes from 6 patients with 3 types of genetic mucopolysaccharidoses (hurler's syndrome, hunter's syndrome and morquio's syndrome) contained numberous vacuoles in their cytoplasm. the size of the vacuoles ranged from approximately 300 nm to 750 nm. the percentage of the lymphocytes with vacuoles varied from 10% to 38%. the vacuoles showed acid phosphatase activity, which indicated their lysosommal nature. staining with dialyzed iron solution usually localized acid mucosubstance in the perip ... | 1977 | 412306 |
| differential termination of large-diameter and small-diameter primary afferent fibers in the spinal dorsal gray matter as indicated by labeling with horseradish peroxidase. | 1977 | 19605029 | |
| a simple apparatus for the injection of small (nanolitre) volumes of horseradish peroxidase. | 1977 | 19605023 | |
| peroxidase and gold complexes of lectins for double labeling of surface-binding sites by electron microscopy. | double labeling experiments were performed for visualization of the binding sites of concanavalin a and anti-ahel (the lectin from helix pomatia). the anti-ahel was labeled with the colloidal gold whereas the membrane bound concanavalin a was demonstrated by an affinity technique using horseradish peroxidase. the two markers used could be clearly distinguished electron microscopically. the specificity of the cell surface double labeling was demonstrated in the control experiments. a topological ... | 1977 | 915241 |
| the nucleus corporis pontobulbaris of the north american opossum. | the nucleus of the pontobulbar body (pbu) in the north american opossum is located, for the most part, adjacent to the motor root of the trigeminal nerve. material prepared by degeneration and autoradiographic methods shows that the pbu receives projections from the facial motor-sensory cortex, red nucleus, spinal cord and cerebellum. the latter fibers probably take origin within the fastigial nucleus. each of the afferent connections ends in a restricuted part of the pbu, but there is considera ... | 1977 | 903428 |
| concanavalin a-binding by cells of the early chick embryo. | the surfaces of cells from the early embryo of the chick were examined using electron microscope techniques for the visualization of concanavalin a-binding sites. horseradish peroxidase and ferritin labelled concanavalin a were used to determine the distribution of the binding sites. all surfaces of the epiblast and hypoblast layers which were accessible to concanavalin a showed the presence of binding sites in stage 1 embryos. the ventral surface of the epiblast showed a high lectin affinity wh ... | 1977 | 561795 |
| kinetics of antibody and immunoglobulin-producing cells appearing in popliteal lymph nodes of mice stimulated with horseradish peroxidase. | 1977 | 561126 | |
| horseradish peroxidase transport from primate dental pulps. | 1977 | 75216 | |
| an electron microscopic investigation of the surface coat of the electrocyte of electrophorus electricus. | the surface coat of the electrocyte of the main electric organ of electrophorus electricus was studied using cytochemical methods (periodic acid-silver methanamine, periodic acid-chromic acid-silver methenamine, periodic acid-thiosemicarbazide-silver proteinate, concanavalin a - horseradish peroxidase, ruthenium red, alcian-blue lanthanum nitrate, colloidal iron hydroxide and cationized ferritin). the surface of the electrocyte presents perpendicularly oriented tubular invaginations of the cell ... | 1977 | 72610 |
| cytochemical localization of acetylcholine receptors at the neuromuscular junction by means of horseradish peroxidase-labeled alpha-bungarotoxin. | the cytochemical localization of acetylcholine (ach) receptors at the neuromuscular junction was investigated with a procedure utilizing alpha-bungarotoxin (alpha-btx) labeled directly with horseradish peroxidase (hrp). following incubation of tissues in the conjugate and reaction for peroxidase, activity was observed on the junctional folds of the motor endplate. a uniform layer of reaction product approximately 15 nm thick occurred over the apical portions of the junctional folds. membranes at ... | 1977 | 912399 |
| vestibular projections to the nucleus intercalatus of staderini mapped by retrograde transport of horseradish peroxidase. | medullary afferent projections to the nucleus intercalatus of staderini have been studied by retrograde transport of horseradish peroxidase (hrp) from highly localized injections. this nucleus receives afferent projections particularly from the medial and descending vestibular nuclei as well as from the nucleus praepositus hypoglossi of both sides. the nucleus intercalatus of staderini represents therefore an area of integration for the vestibular systems of both sides. | 1977 | 19605012 |
| electron microscopic labeling of intercellular spaces in isolated tissue specimens with horseradish peroxidase. | 1977 | 929637 | |
| magnetic circular dichroism studies on acid and alkaline forms of horseradish peroxidase. | the heme vicinities of the acid and alkaline forms of native (fd(iii)) horseradish peroxidase were investigated in terms of the magnetic circular dichroism (mcd) spectroscopy. the mcd spectrum of the acid form of native horseradish peroxidase was characteristic of a ferric high spin heme group. the resemblance in the mcd spectrum between the acid form and acetato-iron (iii)protoporphyrin ix dimethyl ester suggests that the heme iron of the acid form has the electronic structure similar to that i ... | 1977 | 19085 |
| a perfusion method of incubation to demonstrate horseradish peroxidase in bone. | a perfusion method of incubation to show horseradish peroxidase in the bone of young mice is presented. after perfusion fixation, the incubation medium is perfused from the descending aorta into the entire lower half of the animal. from the vessels there is good penetration of the medium into all tissues. this allows the preparation of any one perfused bone to ground-, semithin-, and ultrathin sections. differences in peroxidase distribution in the entire bone suggest regional differences in vas ... | 1977 | 914657 |
| the connections of the trigeminal and facial motor nuclei in the brain of the carp (cyprinus carpio l.) as revealed by anterograde and retrograde transport of horseradish peroxidase. | the connections of the rostral and caudal parts of the trigeminal and facial motor nuclei in the carp were studied with the horseradish peroxidase technique. following ionophoretic peroxidase injections in these motor nuclei, retrogradely labeled cells were observed together with anterogradely labeled motor cell processes. several cellular areas in thalamus, cerebellum and medulla oblongata were shown to project to the v and vii motor nuclei. labeled cells were found in the inferior lobe and the ... | 1977 | 903419 |
| anatomical and physiological characterization of avian sympathetic cardiac efferents. | in the preceding paper6 describing the right sympathetic cardiac nerve of the pigeon we reported a fast compound action potential component with no chronotropic effect, and single unit recordings from sympathetic postganglionic neurons demonstrated that this action potential component was not reflected in the antidromic postganglionic latencies. electron microscopy then indicated the presence of numerous myelinated fibers in the nerve, and together these findings suggested the existence of a sub ... | 1977 | 884549 |
| nuclear magnetic resonance studies of hemoproteins. interaction of horseradish peroxidase with indolepropionic acid. | 1977 | 891962 | |
| foveal sparing. new anatomical evidence for bilateral representation of the central retina. | the pattern of retinal ganglion cell projections to the dorsal lateral geniculate nuclei has been demonstrated with horseradish peroxidase neuronography. a 1 degree-wide strip centered on the vertical meridian has been found in which ipsilaterally and contralaterally projecting ganglion cells intermingle. this strip expands to a width of 3 degrees at the fovea, since mixing of horseradish peroxidase-labeled and unlabeled ganglion cells was found in a band approximately 0.5 degrees wide along bot ... | 1977 | 407893 |
| [kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase]. | a kinetic study of o-dianisidine oxidation by hydrogen peroxide in the presence of horseradish peroxidase within the ph range of 3.7-9.0 has been carried out. it was shown that the reaction of o-dianisidine peroxidase oxidation obeys the michaelis--menten kinetics; the kcat and km values within the ph range used were determined. the optimum of peroxidase catalytic activity during o-dianisidine oxidation was observed at ph 5.0-6.0. the kinetic pattern of the reaction is discussed. it was demonstr ... | 1977 | 20989 |
| [the reaction of holo-, apo- and coenzyme of the peroxidase to heating (author's transl)]. | the thermal reaction of the two components of the horseradish peroxidase--the apoenzyme and the prosthetic group--with that of the holoenzyme were compared. from this we conclude that the thermal inactivation of the peroxidase in aqueous solution is not caused by a change of the apoprotein alone, but rather by participation of the entire three-dimensional structure including the prosthetic group. spectrophotometric studies of the inactivation process in the holoenzyme revealed that the absorbanc ... | 1977 | 910551 |
| ultrastructural localization of a nerve growth factor-horseradish peroxidase (ngf-hrp) coupling product after retrograde axonal transport in adrenergic neurons. | 1977 | 69476 | |
| perihypoglossal and secondary vestibular projections to lobules vi and vii of the cerebellar cortex: an hrp study. | horseradish peroxidase (hrp) was injected into (1) lobule vi; (2) lobule vii; (3) lobule vi and vii of the cat's cerebellar vermis. marked cells were found in two groups of nuclei related to the vestibulo-ocular system; (a) the three perihypoglossal nuclei: praepositus hypoglossi, nucleus of roller and intercalatus of staderini and (b) two areas of the secondary vestibular complex located within the medial and the descending vestibular nuclei. it is concluded that fibers from the perihypoglossal ... | 1977 | 19604979 |
| an enzyme-linked immunoassay of testosterone. | an enzyme-linked immunoassay of testosterone is described. the conjugation of testosterone with high specific activity horseradish per-oxidase and a highly sensitive assay for this enzyme having previously been studied, here we describe the immobilization of the anti-testosterone antibody and the development of assay conditions permitting the determination of 50 pg to 1.5 ng testosterone in one working day. in this work attention has been paid to keeping the assay method as simple as possible. t ... | 1977 | 919013 |
| characterization of the pigeon isthmo-tectal pathway by selective uptake and retrograde movement of radioactive compounds and by golgi-like horseradish peroxidase labeling. | 1977 | 69469 | |
| effect of mixed solvents on the formation of horseradish peroxidase compound i. the importance of diffusion-controlled reactions. | in aqueous glycerol solutions, the rate constant for the formation of compound i of horseradish peroxidase from hydrogen peroxide is independent of the viscosity. however, whem m-chloroperbenzoic acid is used to form compound i, the rate is viscosity dependent, indicating that this reaction is diffusion controlled. the problems in obtaining accurate information about diffusion-controlled reactions are discussed as is the possible significance of diffusion control. ethanol has been used in previo ... | 1977 | 880290 |
| slow rate transport of horseradish peroxidase in toads. | 1977 | 69468 | |
| automated fluorometric analysis of galactose in blood. | in galactosemia, prevention of mental retardation depends on early recognition of the disorder and institution of dietary restriction of galactose. we describe an automated fluorometric micromethod for galactose in whole blood spotted on filter paper. galactose is oxidized by galactose oxidase to d-galacto-hexadialdose and h2o2 and measured as the highly fluorescent condensation product of homovanillic acid formed when h2o2 is acted upon by horseradish peroxidase. the procedure is 10-fold more s ... | 1977 | 870260 |
| [thermal inactivation and storage behavior of technologically important enzymes. iii. effect of reagents added to peroxidase and lipoxygenase]. | the influence of milieu factors on the thermal inactivation of peroxidase and lipoxigenase was investigated. cationogenic, anionogenic, non-ionogenic and amphoteric tensides were more or less effective in inactivating horseradish peroxidase. most effective in this respect were lecithine and monoglyceride, both capable of swelling in water. in presence of lecithine, peroxidase was inactivated already at 0 degrees c and ph 4.0. linoleic acid was more efficient in an oxygen stream than in presence ... | 1977 | 408986 |
| histochemical and ultrastructural studies in fibrodysplasia ossificans progressiva (myositis ossificans progressiva). | by light microscopy the subdermal nodule of a patient with fibrodysplasia ossificans progressiva (fop) had a fibromatoid histologic appearance. the cytoplasm of the cells stained strongly for mannose-rich glycoprotein with the concanavalin a-horseradish peroxidase (con a-hrp) method. the tumors also exhibited abundant hyaluronidase-digestible mucopolysaccharide in the interstitium with various basic staining reagents. this material appeared to consist principally of hyaluronic acid or chondroiti ... | 1977 | 141214 |
| [thermal inactivation and storage behavior of technologically important enzymes. i. horseradish and spinach peroxidase]. | the thermal inactivation and storage behaviour for horseradish and spinach peroxidases were investigated in defined systems, in spinach also within its natural environment. the inactivation curves of either enzyme show a sharp bend which is clearly visible at low, but not at higher temperatures. the d-values were taken from the inactivation curves. z-values resulting from the d-values were 25.5 degrees c for horseradish peroxidase, 13 degrees c for isolated peroxidase and 18 degrees c for peroxi ... | 1977 | 19887 |
| evaluation of the horseradish peroxidase-scopoletin method for the measurement of hydrogen peroxide formation in biological systems. | 1977 | 883627 | |
| the organization of the pulvinar in the grey squirrel (sciurus carolinensis). i. cytoarchitecture and connections. | the posterior neocortex in the grey squirrel, sciurus carolinensis, includes an extensive region which receives projections from the pulvinar. previous studies have demonstrated that this cortical region can be subdivided on the basis of differences in cytoarchitecture and electrophysiologically defined representations of the visual field. the main purpose of the present paper was to determine whether these cortical subdivisions could be related to corresponding subdivisions in the pulvinar. the ... | 1977 | 856889 |
| a new and sensitive staining method for axonally transported horseradish peroxidase (hrp) in the pigeon visual system. | 1977 | 67876 | |
| active site models of horseradish peroxidase compound i and a cytochrome p-450 analogue: electronic structure and electric field gradients. | 1977 | 853195 | |
| mechanism of thyroxine-mediated oxidation of reduced nicotinamide adenine dinucleotide in peroxidase-h2o2 system. | the oxidation of reduced nicotinamide adenine dinucleotide (nadh) by the horseradish peroxidase (hrp)-h2o2 system is greatly increased by the addition of thyroxine or related compounds. on the basis of a study of the rate of nadh oxidation in the presence of various concentrations of thyroxine, it is clear that thyroxine acts as a catalyst for nadh oxidation. spectral changes of a hrp-h2o2 complex (compound i) indicate that thyroxine acts as an electron donor to both compounds i and ii. the rate ... | 1977 | 192281 |
| analysis and computer simulation of aerobic oxidation of reduced nicotinamide adenine dinucleotide catalyzed by horseradish peroxidase. | under suitable experimental conditions the aerobic oxidation of nadh catalyzed by horseradish peroxidase occurred in four characteristic phases: initial burst, induction phase, steady state, and termination. a trace amount of h2o2 present in the nadh solution brought about initial burst in the formation of oxyperoxidase. about 2 mol of oxyperoxidase was formed per mol of h2o2. when a considerable amount of the ferric enzyme still remained, the initial burst was followed by an induction phase. in ... | 1977 | 192280 |
| [catalytic properties and thermostability of horseradish peroxidase covalently bound with sepharose by carbohydrate residues of the enzyme]. | a procedure for isolation of stabilized preparations of peroxidase, covalently bound with ah-sepharose 4b by the enzyme carbohydrate residues, which had been previously oxidized by iodine acid, is proposed. the catalytic properties and thermostability of soluble oxidized and immobilized peroxidase has been studied and a comparison of these properties with those of the native enzyme has been made. it has been demonstrated that the oxidation of the carbohydrate residues dose not affect eh km value ... | 1977 | 889967 |
| [development of arrangement and degradation of the system of rough endoplasmic reticulum in antibody-producing plasma cells]. | during the immune response of the cells of murine omentum to horseradish peroxidase which had been administered intraperitoneally together together with endotoxin or endotoxoid acting as an adjuvans the plasma cells showed several different patterns of the rough endoplasmic reticulum [rer] system. irregular to bizarre rer structures occurred plasma cells during the initial phase of the immune reaction only, whereas the plasma cells found at later stages had characteristic highly organized rer sy ... | 1977 | 597922 |
| retrograde axonal transport of horseradish peroxidase from cornea to trigeminal ganglion. | horseradish peroxidase (hrp) was dripped on the scarified left cornea of adult mice. twenty-four hours later the animals were fixed by vascular perfusion and frozen sections cut from both trigeminal ganglia. after incubation for peroxidase activity labelled nerve cells were restricted to the medial ophthalmic part of the ganglion ipsilateral to hrp administration. if the scarification was omitted no neuronal labelling was observed. this labelling of the neurons is most probably the result from a ... | 1977 | 300978 |
| plant glycoprotein biosynthesis. uridine diphosphate n-acetyl-glucosaminyltransferase from horseradish root. | a particulate enzyme preparation from horseradish root tissue was shown to catalyze the transfer of 2-acetamido-2-deoxy-d-[14c1]glucose from uridine diphosphate 2-acetamido-2-deoxy-d-[14c1]glucose to an exogenous acceptor molecule derived from horseradish peroxidase. the acceptor was produced from purified peroxidase by the action of a mixture of glycoside hydrolases covalently bound to sepharose. the membrane preparation containing the transferase was purified approximately 12-fold by aqueous t ... | 1977 | 403958 |
| polymerisation of oestradiol by potassium ferricy anide and by horseradish peroxidase. | 1977 | 862904 | |
| vesicular transport of horseradish peroxidase from brain to blood in segments of the cerebral microvasculature in adult mice. | the transfer of protein from the cerebral ventricles to the parenchymal bloodstream in mice was studied by electron microscopy. after perfusion with the protein tracer horseradish peroxidase (hrp; m.w. approx. 40,000) through the cerebral ventricles, the tracer penetrated the ependymal lining of the ventricles and was found in the extracellular space of the neuropil close to the ependyma. hrp was also seen in the vascular basement membrane and in endothelial vesicles opening at the abluminal end ... | 1977 | 843935 |
| amino-acid sequences of heme-linked, histidine-containing peptides of five peroxidases from horseradish and turnip. | in a previous paper we have characterized five plant peroxidases, p1, p2, p3 and p7 of turnip and horseradish isoperoxidase c by peptide mapping studies, and only found two highly homologous sequences present in all. both contained histidine. the findings supported previous suggestions of two histidine sequences nearthe peroxidase heme prostetic group. in the present paper we present the amino acid sequences around the histidine residues of all four turnip peroxidases, i. e. of 25 residues aroun ... | 1977 | 849740 |
| the organization of the facial nucleus of the brush-tailed possum (trichosurus vulpecula). | the facial nucleus of the brush-tailed possum has been studied using nissl staining and the horseradish peroxidase (hrp) retrograde tracing technique. in nissl stained sections the nucleus is seen to comprise five distinct subnuclei. injections of hrp into individual facial muscle groups have shown that these subnuclei reflect the peripheral innervation pattern of efferents from this nucleus. although in most cases, injection of hrp into a single facial muscle group resulted in the labelling of ... | 1977 | 65363 |
| efferent projections of the main and the accessory olfactory bulb in the tree shrew (tupaia glis). | the projections of the main and the accessory olfactory bulb in the tree shrew (tupaia glis) have been analyzed with anterograde degeneration and autoradiographic methods for identifying axonal projections, and with the horseradish peroxidase method for identifying the distribution of neurons from which these projections originate. the cytoarchitectonic features of the paleocortical areas which receive projections from the main and the accessory olfactory bulb have also been described. the effer ... | 1977 | 65361 |
| free radical intermediate in the n-demethylation of aminopyrine by horseradish peroxidase-hydrogen peroxide. | 1977 | 190040 | |
| sensitized photooxidation of low spin horseradish peroxidase. | 1977 | 191009 | |
| electron microscopic observations of cell coat of childhood leukemic cells using concanavalin a-horseradish peroxidase method. | 1977 | 327742 | |
| horseradish peroxidase. xxv. an electron spin resonance study of the low temperature photochemical reaction of compound i. | 1977 | 189760 | |
| protein-accumulating cells and dilated cisternae of the endoplasmic reticulum in three glucosinolate-containing genera: armoracia, capparis, drypetes. | three glucosinolate-containing species, armoracia rusticana gaertner, meyer et scherbius (brassicaceae), capparis cynophallophora l. (capparaceae) and drypetes roxburghii (wall.) hurusawa (euphorbiaceae), are shown by both light and electron microscopy to contain protein-accumulating cells (pac). the pac of armoracia and copparis (former "myrosin cells") occur as idioblasts. the pac of drypetes are usual members among axial phloem parenchyma cells rather than idioblasts. in drypetes the vacuoles ... | 1977 | 24420656 |