Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [effect of gamma radiation on the immunobiological and immunochemical properties of cholera exotoxin. iii. the serological activity and immunochemical properties of irradiated unpurified toxin]. | the immunochemical properties and serological activity of irradiated preparations of crude cholera exotoxin have been studied. this study has revealed that with the increase of the dose of ionizing radiation changes occur in the physico-chemical properties of the preparations of the toxin, which leads to an increase in the electrophoretic motility of the protein components of the toxin, to the aggregation and polymerization of individual fragments. the preparations of antigen exotoxins have been ... | 1984 | 6380166 |
| detection of campylobacter by immunofluorescence in stools and rectal biopsies of patients with diarrhoea. | rabbit antiserum, elicited by the intravenous injection of a strain of campylobacter jejuni heated to 100 degrees c, cross reacted strongly with all other thermophilic campylobacters tested as well as with "c pyloridis" and could be detected by indirect fluorescence with labelled anti-rabbit serum. antisera to formalin killed cells did not do so. the correlation of positive stool culture with positive immunofluorescence of stools and rectal biopsies from patients with diarrhoea was 70-80%. some ... | 1984 | 6381550 |
| robert koch and the cholera vibrio: a centenary. | 1984 | 6419937 | |
| toxin receptors and their pathogenetic significance. | the pathogenetic significance of toxin receptor interaction in disease is examplified by infectious diarrhoea. the world-wide problem of infectious diarrhoea is presented with regard to epidemiology, etiology, and pathophysiology. enterotoxigenic diarrhoea is examined in the light of recent knowledge on toxin receptor interaction, which has greatly contributed to new vaccine as well as drug development in this disease condition. | 1984 | 6425929 |
| [inhibition of premature uterine contractions]. | various drugs used to stop premature uterine contractions are discussed in the paper. particular attention is paid to beta-mimetic drugs. the results of ming partusisten, one of beta-mimetic drugs, is presented on the material of 104 patients with threatening immature and premature labour. partusisten was administered in the form of intravenous drip infusion or tablets. during treatment monitoring of the uterine contractility and of foetal heart rate took place. inhibition of the uterine contrac ... | 1984 | 263521 |
| the kidney in sickle cell anemia. | 1984 | 240053 | |
| [occurrence of atypically shaped teeth in patients with cleft palate]. | 1984 | 271542 | |
| intraocular lens implants. an introduction. | 1984 | 271406 | |
| trampolines and triangles. the surgical pathology of the vitreous. | the vitreous cavity may be invaded by cells penetrating through dehiscences in the inner limiting lamina of the retina, and exaggerated vitreo-retinal adhesions may also develop at such sites. the vitreous often separates from the inner limiting lamina except at these anomalous adhesions, and retinal traction thus results from excessive movement of the otherwise detached gel; traction on the retina may also follow proliferation and contraction of invading cells on the retinal surface, along the ... | 1984 | 345530 |
| phase behavior of ganglioside-lecithin mixtures. relation to dispersion of gangliosides in membranes. | ganglioside gm1 and mixed brain gangliosides were mixed with 1-stearoyl-2-oleoyl lecithin (sopc) and examined by differential scanning calorimetry as a function of ganglioside content and temperature. low mole fractions of ganglioside gm1 and of mixed brain gangliosides are shown to be miscible with sopc in the gel phase up to x = 0.3, with the possible exception of a small region of immiscibility for the mixed brain gangliosides system centered around x = 0.05. above x = 0.3, the low-temperatur ... | 1984 | 263689 |
| [clinical and pathomorphological evaluation of malignant epithelial odontogenic tumors]. | 1984 | 271067 | |
| cyclosporin-induced renal magnesium leak in renal transplant patients. | renal transplant patients (n = 116) attending an outpatients clinic were screened for hypomagnesaemia. no azathioprine-treated patients (n = 46) but 24% (17 of 70) of the cyclosporin treated patients were hypomagnesaemic. the hypomagnesaemia in all cases was associated with a renal magnesium leak. this leak did not correlate with plasma bicarbonate, urate, calcium, cyclosporin or creatinine concentrations, nor did it correlate with the use of loop diuretics or duration of the transplant. a renal ... | 1984 | 2129356 |
| a method of obturation of an oral-cutaneous fistula. | 1984 | 264321 | |
| use of metrizamide in coronary arteriography. | in the investigation of the arteriae coronariae by means of angiography, a comparative study has been done of two contrast media in the same patients, by injecting these media in a randomized order. the contrast media are sodium methylglucamine diatrizoate (10:66) and metrizamide, both used in a concentration of 370 mg i/ml. the reaction of the heart was registered in different ways with which also the influence on the blood pressure was measured. the quality of the films was judged by persons w ... | 1984 | 498916 |
| metacarpophalangeal arthroplasty. | 1984 | 261543 | |
| effects of nutrient deprivation on vibrio cholerae. | [this corrects the article on p. 931 in vol. 46.]. | 1984 | 16346550 |
| [determination of the initial localization of the growth of disseminated malignant tumors in the nasal cavity and paranasal sinuses]. | 1984 | 425256 | |
| air pollution in the region of kafue industrial estate--a preliminary report. | 1984 | 263371 | |
| urinary 5 alpha-androstane-3 alpha,17 beta-diol radioimmunoassay: a new clinical evaluation. | a rapid specific and reliable ria for urinary 5 alpha-androstane-3 alpha,17 beta-diol (adiol) is described using chromatographical purification and a specific antibody. values are reported under some physiological and pathological conditions in 179 individuals. in 43 normal adult men, the mean (+/-sd) urinary adiol excretion was 193 +/- 77 micrograms/24 h, and in 29 normal women it was 44 +/- 23 micrograms/24 h. these values are significantly different (p less than 0.01). in 49 hirsute women, ur ... | 1984 | 263329 |
| over-all view of neurosurgical manpower and training. | 1984 | 241529 | |
| [health care sociology]. | 1984 | 254414 | |
| effects of chlordiazepoxide, cns stimulants and their combinations on avoidance behaviour in mice. | various central stimulant drugs were tested in mice subjected to five daily 100-trial avoidance sessions in the shuttle-box. facilitation of avoidance responding was observed following i.p. administration of metamphetamine and cocaine, but not following methlyphenidate, caffeine, pentetrazole and strychnine. some favourable effects were obtained by combining the above stimulant drugs with cholrdiazepoxide. advantages in the combination with chlordiazepoxide were particularly evident in the case ... | 1984 | 240332 |
| [treatment of the rural population by feldshers]. | 1984 | 240204 | |
| good bites, bad bites, and malpractice suits. | 1984 | 271163 | |
| [results of the surgical treatment of children with residual developmental anomalies of the 1st and 2d branchial clefts and thyroglossal duct]. | the purpose of this study was assessment of the results of surgical treatment of children with developmental anomalies derived from the first and second branchial clefts and thyroglossal duct. normal and abnormal development of branchial arches and clefts, pharyngeal pouches and thyroglossal duct, as well as the anatomy nd topography of the resulting developmental anomalies are discussed. the observed developmental anomalies are classified into: cysts, cysts with sinuses developing as a result o ... | 1984 | 263538 |
| [clinical and biochemical diagnosis of galactosemia among our cases]. | clinical and biochemical diagnostic studies concerned 17 cases of galactosemia coming from 15 not consauguineous families. galactosemia was diagnosed between 1-st day and 11-th month of life. tentative diagnosis based on clinical picture was made in 12 infants, others were detected through family history of galactosemia and/or biochemical newborn screening carried out at the national research institute of mother and child since 1969. clinical symptoms of galactosemia occurred in most patients in ... | 1984 | 263527 |
| guanosine diphosphate-l-fucose plasma: n-acetylglucosaminide fucosyltransferase as in index of bone marrow hyperplasia after chemotherapy. | we have measured the plasma level of a fucosyltransferase in patients with acute myelogenous leukemia and non-hodgkin's lymphoma at various stages of the disease and in normal controls. this enzyme transfers the sugar fucose from a guanosine diphosphate-l-fucose donor to high-molecular-weight acceptors with a terminal n-acetyl-glucosamine residue. the enzyme levels of fucosyltransferase in individuals free from disease and in patients with untreated leukemia or lymphoma were comparable. a substa ... | 1984 | 271043 |
| refractory anaemia with excess of myeloblasts in the bone marrow: a clinical trial of androgens in 90 patients. | in a cooperative trial, 90 patients with refractory anaemia with an excess of blast cells in the marrow were evaluated and treated with androgens. the clinical presentation was very similar to previously published observations: features of medullary insufficiency were less marked than in primary aplastic anaemia; bone marrow blastic infiltration varied from case to case, and remained stable until death or until an acute leukaemic change. all the patients were treated with high doses of androgens ... | 1984 | 271013 |
| supernumerary deciduous lateral incisor in a cleft lip and palate patient. case report. | 1984 | 264327 | |
| [conservative treatment of patients with small cell lung cancer]. | 1983 | 184622 | |
| responses to salvage chemotherapy in ovarian cancer: a critical need for precise definitions of the treated population. | 1983 | 1548513 | |
| an analysis of admissions procedures in a dental school. | 1983 | 264477 | |
| the dentist and the battered child syndrome. | 1983 | 264457 | |
| an expanded model for extramural dental clinics. | the extramural program at the university of the pacific school of dentistry is described. the program is designed to facilitate the students' transition from a school clinic to dental practice after graduation. management skills are taught by providing supervised experience in a model practice setting. five major facilities are described along with the curriculum modifications made to permit all students to spend eight four-day weeks in these remote sites. the quality control mechanism is discus ... | 1983 | 264892 |
| [aleksandr fedorovich makarchenko]. | 1983 | 393534 | |
| [study of lichen planus by direct immunofluorescence (author's transl)]. | the results of direct immunofluorescent staining from patients with different types of lichen planus are reported. total antiserum, iga, igg, igm, c1b/c1a and fg antiserums were used. immunoglobulin deposits were noted on colloid bodies, on the epidermal basement ,nd the walls of the papillary vessels. | 1983 | 348005 |
| phenformin acidosis. | 1983 | 272564 | |
| allies in health. | 1983 | 264623 | |
| financial impact of physician assistants on medical practice. | a study of revenues generated and expenses incurred by 12 physician assistants (medex) who had held salaried positions for at least one year was conducted to determine their financial impact on primary-care practices. daily charge logs were used to make annual estimates of medex-generated revenues. one method of estimating annual revenues produced a mean of $28,190 per year, and a second method yielded a figure of $30,210 per year. financial statements were used in two different ways to estimate ... | 1983 | 239338 |
| [certain peculiarities in the phenomenology and therapy of jaw osteomyelitis]. | 1983 | 272810 | |
| [in memory of vladimir vladimirovich kuntsevich]. | 1983 | 348161 | |
| kinetic and equilibrium studies of cyanide and fluoride binding to turnip peroxidases. | 1983 | 242258 | |
| enzyme-linked immunoassays for antibodies against vibrio cholerae. | glutaraldehyde-treated v. cholerae organisms bind firmly to the surfaces of plastic microelisa plates, thus providing a stable immobilized antigen for use in enzyme-linked immunosorbent assays (elisa). serum absorption and elisa-inhibition experiments indicate that, in addition to detecting natural antibodies in normal rat serum, the immobilized antigen may be used to quantitate specific anti-v. cholerae antibodies induced in rats by injection of live organisms. apart from serotypically specific ... | 1983 | 6426450 |
| food microbiology update. emerging foodborne pathogens. | a review of three "emerging" foodborne pathogen groups is presented, including campylobacter jejuni/coli, yersinia enterocolitica, and foodborne vibrio sp. | 1983 | 6383214 |
| comparative behaviour of classical and el tor biotypes of vibrio cholerae 01 isolated in bangladesh during 1982. | the change from classical to the el tor biotype of vibrio cholerae during the early seventies in bangladesh remains to be elucidated. the shift in 1982 from el tor back to the classical was not predicted, but provided an opportunity to study both the biotypes occurring simultaneously in nature, compared with earlier isolates. comparative studies showed tcbs to be as good as ttga for isolation of the 2 biotypes. replacement of the el tor by the classical biotype as the dominant epidemic strain ... | 1983 | 6384354 |
| the suitability of coagglutination for the rapid bacteriological diagnosis of vibrio cholerae 0:1. | 1983 | 6380449 | |
| a longitudinal study of the prevalence of bacterial enteric pathogens among adults with diarrhea in bangkok, thailand. | six hundred sixty adults with diarrhea treated at bamrasnaradura hospital, bangkok, thailand were investigated to determine the prevalence, seasonality, and severity of diarrhea associated with bacterial enteric pathogens in 1980 and 1981. shigella were isolated from 27% and vibrio parahaemolyticus from 19% of the patients studied. enterotoxigenic escherichia coli (5%), salmonella (3%), non-01 vibrio cholerae (3%), campylobacter jejuni (1%), and group f vibrio (less than 1%) were isolated from a ... | 1983 | 6370564 |
| immunochemical properties of the major outer membrane protein of vibrio cholerae. | antisera to the major outer membrane protein of vibrio cholerae (molecular weight, 48,000) raised in rabbits (i) agglutinated several strains of v. cholerae and (ii) immunoprecipitated outer membrane proteins prepared from both the biotypes and serotypes of v. cholerae. antibodies of all isotypes to the major outer membrane protein were detected in immune human sera by enzyme-linked immunosorbent assay. these results suggest that the major outer membrane protein was the common outer membrane ant ... | 1983 | 6822427 |
| medium-dependent production of extracellular enterotoxins by non-o-1 vibrio cholerae, vibrio mimicus, and vibrio fluvialis. | fluid accumulation at 4 h in the intestines of suckling mice enabled us to distinguish non-o-1 vibrio cholerae, v. mimicus, and v. fluvialis clinical isolates from environmental isolates. enterotoxin production was culture medium dependent. filtrates of cultures grown in tryptic soy broth without glucose but with added 0.5% nacl did not exhibit marked enterotoxin activity in the assay. culture filtrates of all clinical strains grown in brain heart infusion broth supplemented with 0.5% nacl induc ... | 1983 | 6824317 |
| isolation of vibrio cholerae serotype ogawa from a florida estuary. | vibrio cholerae serotype ogawa was recently isolated from the estuarine waters of apalachicola bay, fla., in areas that are subject to consistent fecal contamination and in areas that are remote from any apparent source of contamination. the significance of these organisms in the environment has not been determined. | 1983 | 6824323 |
| plasmid-borne multiple drug resistance in vibrio cholerae serogroup o1, biotype el tor: evidence for a point-source outbreak in bangladesh. | in 1979, an outbreak of plasmid-borne, multiply drug-resistant vibrio cholerae serogroup o1 biotype el tor (v. cholerae o1) occurred in the matlab area of bangladesh. the outbreak could have resulted from the introduction into the area of a single resistant strain or from multiple conjugations of drug-sensitive v. cholerae o1 with c plasmids in other environmental flora. resistant strains were phage typed to determine their relatedness, and plasmid studies were conducted to determine the frequen ... | 1983 | 6827137 |
| migrating action-potential complexes in vitro in cholera-exposed rabbit ileum. | the objective of this study was to determine whether cholera-exposed rabbit ileum exhibits altered myoelectric activity in vitro, without central nervous system connections. whole-cell lysate of vibrio cholerae, 100 mg in 1 ml saline, was injected into the jejunum of new zealand white rabbits. segments of ileum were removed at 12 and 24 h after inoculation and studied in vitro using myoelectric recording techniques. propagating ring contractions were visualized and corresponded to intense action ... | 1983 | 6829769 |
| temperature-mediated interaction of tetanus toxin with cerebral neuron cultures: characterization of a neuraminidase-insensitive toxin-receptor complex. | energy-dependent internalization of 125i-labeled tetanus toxin into cultured neural cells is shown to follow an energy-independent binding process. a three-step model, involving receptor-mediated binding followed by sequestration and internalization is proposed. in the first step, binding of toxin is enhanced in appearance under low ionic strength medium, at 0-4 degrees c; it is suppressed, however, with increasing incubation temperature under physiological salt concentrations. cell-bound toxin ... | 1983 | 6834057 |
| radiation-sensitive mutant of hypertoxinogenic strain 569b of vibrio cholerae. | a radiation-sensitive mutant of the hypertoxinogenic strain 569b of vibrio cholerae was isolated and characterized. the mutant, designated v. cholerae 569bs, lacks both excision- and medium-dependent dark-repair mechanisms of uv-induced dna damage while retaining the wild-type photoreactivating capability. analysis of the uv-irradiated cell dna by velocity sedimentation in alkaline sucrose gradient suggests that uv-induced pyrimidine dimers may not be incised in these cells. in contrast to the w ... | 1983 | 6835234 |
| [relationship between hemolysin and hemolysin-destructive factor activities in vibrio cholerae of eltor biovar]. | the study of hemolysin activity, hemolysin-destructive factor (hdf) activity and cholerogenicity in 143 vibrio eltor strains has revealed the existance of close relationship between cholerogenicity and hemolysin production, as well as between cholerogenicity and hdf activity. the negative character of conjugation between the hdf activity and the hemolytic activity of the strains under study has been established, which allows one to suggest the possibility of the regulating action of hemolysin on ... | 1983 | 6837203 |
| thiosulfate-citrate-bile salts-sucrose agar and its selectivity for clinical and marine vibrio organisms. | recently documented vibrio cholerae and non-cholera vibrio infections in florida and other american waters led to a study to determine the selectivity of thiosulfate-citrate-bile salts-sucrose media (tcbs). thirty-one species with 188 different strains of clinical, marine, and stock origin were examined on the tcbs media. one hundred seventy-seven of the 188 strains grew. nine species had the typical yellow colony appearance that might be confused with v. cholerae and nine species were green, po ... | 1983 | 6838150 |
| the role of sialic acid in the dysfibrinogenemia associated with liver disease: distribution of sialic acid on the constituent chains. | to further evaluate the role of sialic acid in the dysfibrinogenemia associated with liver disease, we studied the effect of removal of excess sialic acid residues from the fibrinogen of five patients with liver disease on the thrombin time and fibrin monomer aggregation. patient fibrinogens containing 1.4-3.4 residues of sialic acid per molecule in excess of normal controls, with thrombin times 12-22 sec longer than normal and with abnormal fibrin monomer aggregation, were stripped of their exc ... | 1983 | 6839020 |
| serological characterization of humoral lectins from the freshwater prawn macrobrachium rosenbergii. | we have detected and partially characterized multiple lectins present in the serum of the freshwater prawn macrobrachium rosenbergii. since agglutination of erythrocytes (rbc) is not abolished by treatment with vibrio cholerae neuraminidase (vcn), macrobrachium shows an agglutination pattern different from that of other sialic acid-specific lectins such as limulus polyphemus lectin. however, after absorption with primate and bird vcn-treated rbc, macrobrachium serum exhibits high titers with unt ... | 1983 | 6840378 |
| characterization of surface properties of vibrio cholerae. | a number of isolates of vibrio cholerae were examined with respect to their (i) surface hydrophobicity as measured by hydrophobic interaction chromatography, (ii) capacity to agglutinate erythrocytes, and (iii) ability to bind to an ion-exchange matrix. v. cholerae isolates, cultured under a variety of growth conditions, were conspicuously hydrophobic. the hydrophobicity was accentuated when these strains were (i) cultivated in a chemically defined synthetic medium, (ii) harvested at the exponen ... | 1983 | 6840833 |
| genetic mapping of the tox-1000 locus of vibrio cholerae el tor strain rj1. | the results of a genetic cross between a vibrio cholerae rj1 donor and a v. cholerae 3083-2 recipient suggest that the map position of tox-1000 is between his and trp. | 1983 | 6840862 |
| the role of the flagellum in the adherence of vibrio cholerae. | the role of the flagellar structure in the in vitro adherence and in vivo colonization of vibrio cholerae was studied by comparing the behavior of pairs of related motility variants. although the presence of the flagellum seemed essential for in vitro attachment, the property of motility was neither necessary nor sufficient. since it was possible to demonstrate independently both motility and binding capacities associated with this structure, it was concluded that the flagellum functions as the ... | 1983 | 6842021 |
| the specificity of vibrio cholerae adherence and the significance of the slime agglutinin as a second mediator of in vitro attachment. | the aim of the present studies was to clarify vibrio cholerae adherence by elucidating the basis for the apparently variable nature of in vitro attachment. an examination of the purported specificity of vibrio adherence did not support the concept of host receptor structures; organisms selected for their capacity to adhere to inert substrates simultaneously acquired the capacity to bind to intestinal tissue in vitro. further investigation of the bacterial factors involved in adherence confirmed ... | 1983 | 6842022 |
| protection against cholera in breast-fed children by antibodies in breast milk. | we performed a prospective study to examine whether the iga antibodies against cholera that are present in breast milk protect breast-fed infants and children against colonization with vibrio cholerae 01 and disease. among families of patients with cholera, we collected breast milk from mothers who had not had diarrhea in the previous week and monitored them and their breast-fed children for cholera colonization and diarrhea for 10 days. breast milk was assayed for iga antibodies to cholera toxi ... | 1983 | 6843632 |
| temperate phage induction and filament formation in vibrio cholerae by furazolidone. | furazolidone a synthetic nitrofuran used in the treatment of cholera, was found to cause prophage induction and filament formation in vibrio cholerae. maximum induction of phage beta was obtained at a drug concentration of 0.5 microgram/ml. neither induction of prophage nor filamentation took place if the drug treatment was carried out in the presence of 2.5 micrograms/ml chloramphenicol, indicating a requirement for de novo protein synthesis. our results strongly suggest the existence of "sos" ... | 1983 | 6847681 |
| auto-antibody dependent activation of the autologous classical complement pathway by guinea-pig red cells treated with influenza virus or neuraminidase: in vitro and in vivo study. | guinea-pig erythrocytes that had been exposed to influenza a virus or vibrio cholerae neuraminidase activated the classical complement pathway in autologous serum. because all viral particles were eluted from the treated cells, activation was not dependent on anti-viral antibodies or on the particles themselves. after a threshold of 45-55% desialation, had been reached, the relative capacity of treated cells to activate complement increased very rapidly with desialation. desialation unmasked sit ... | 1983 | 6852870 |
| vibrio factors cause rapid fluid accumulation in suckling mice. | non-o-1 and o-1 vibrio cholerae and vibrio fluvialis isolated from clinical and environmental sources were examined for virulence factor production in 3-day-old suckling mice and in y-1 tissue culture. the responses of the suckling mice to intragastrically administered bacterial cultures were measured by intestinal fluid accumulation (fa), diarrhea, and mortality. regardless of the o-serovar, source of isolation, or ability to produce cholera toxin, all strains of v. cholerae stimulated increase ... | 1983 | 6852913 |
| glycolipids of murine lymphocyte subpopulations. structural characterization of thymus gangliosides. | several sialic acid-containing glycolipids have been isolated and purified from murine thymus. eleven of these acidic glycolipids (gangliosides) have been analyzed by several techniques, permitting us to propose a tentative structure for each. sugar analysis reveals that all 11 gangliosides contain the same base structure, gal(beta 1-3)galnac(beta 1-4)gal(beta 1-4)glc(beta 1-1) ceramide, which is gangliotetraosylceramide. no glucosamine or fucose is present in glycolipids from this tissue. there ... | 1983 | 6853556 |
| [possible reversion of the virulence of vibrio cholerae]. | 1983 | 6855642 | |
| synthesis and secretion of the plasmid-coded heat-labile enterotoxin of escherichia coli in vibrio cholerae. | both cholera toxin and heat-labile enterotoxin were made and secreted into culture supernatants by vibrio cholerae containing the enterotoxin plasmid pcg86. several regulatory mutations in v. cholerae that increased or decreased the synthesis of cholera toxin did not affect production of heat-labile enterotoxin. in contrast, a mutation in v. cholerae that interfered with the secretion of cholera toxin also decreased the secretion of heat-labile enterotoxin, indicating that they are processed by ... | 1983 | 6857285 |
| effects of storage on microbial loads of two commercially important shellfish species, crassostrea virginica and mercenaria campechiensis. | the effects of storage on the microbial load in two commercially important species of shellfish were examined. oysters (crassostrea virginica) were stored as shellstock, shucked meats, and fully processed meats at four temperatures for up to 21 days, and clams (mercenaria campechiensis) were stored only as shellstock. the concentrations of most microbiological groups of organisms increased with the duration and temperature of storage in both shellfish species, although the increases were signifi ... | 1983 | 6859844 |
| differential effect of neuraminidase-treatment on the surface charge-associated properties of rat reticulocytes and erythrocytes. studies by partitioning in two-polymer aqueous phases. | rat reticulocytes undergo charge-associated surface changes, detectable by cell partitioning in charged dextran-poly(ethylene glycol) aqueous phase systems, as they become mature erythrocytes. young reticulocytes have a lower partition coefficient, i.e., quantity of cells in the top phase as a percentage of total cells added, than do mature erythrocytes. sialic acid is the main charge-bearing group on red blood cells and, in the case of the rat, most of the sialic acid can be removed by treatmen ... | 1983 | 6860666 |
| plasmid associated suppression of pathogenicity of wild-type strains of vibrio cholerae from cholera patients. | 1983 | 6862533 | |
| isolation of nontoxigenic vibrio cholerae o1 from a human wound infection. | vibrio cholerae serotype o1 organisms that do not produce cholera toxin and, in fact, lack the genetic material encoding the enterotoxin have recently been detected in coastal regions of the united states. although these organisms have been assumed to be nonpathogenic, they have been considered a potential reservoir of toxigenic v. cholerae. in 1979, nontoxigenic v. cholerae o1 was isolated from a leg wound of an accident victim residing in new orleans. the only known risk factors of the patient ... | 1983 | 6863510 |
| mechanisms for the removal of senescent human erythrocytes from circulation: specificity of the membrane-bound immunoglobulin g. | direct antiglobulin (coombs') tests of erythrocyte (rbc) subpopulations confirmed the presence of membrane-bound immunoglobulin g (igg) on old (density greater than 1.110) human rbcs but not on the young (density less than 1.110) rbcs. after thermal elution of the bound igg, this coombs' reaction was negative, but incubation of thermally eluted igg (he-igg) with heat-treated rbcs induced a positive antiglobulin test. a positive direct antiglobulin reaction was also obtained after incubation of h ... | 1983 | 6865498 |
| transmission of r plasmids in vibrio anguillarum to vibrio cholerae. | 1983 | 6865805 | |
| texas star-sr: attenuated "vibrio cholerae" oral vaccine candidate. | texas star-sr, a laboratory-derived mutant of vibrio cholerae el tor ogawa 3083, which produces b but not a subunit of cholera toxin was given to 68 healthy adult volunteers in doses of 10(5) to 5 x 10(10) organisms. 16 of 68 exhibited loose stools but in only one individual was stool volume notable. vomiting occurred in 1 and abdominal cramps in 3 vaccines; malaise and fever were not seen. texas star was recovered from stools of 22% who received low doses (10(5) or 10(6) organisms) and from 63% ... | 1983 | 6873477 |
| immunogenicity and protective capacity of inactivated "vibrio cholerae" whole cell vaccines. | the effects of heat and chemical inactivants on the immunogenicity and protective capacity of v. cholerae was studied. elisa systems, using whole cells of v. cholerae, purified lps or flagella preparations as immobilized antigens were employed to measure serum antibody titers. the immune response to parenteral vaccination in rabbits with killed whole cell vaccines was dependent upon the inactivation procedure used. cells inactivated by heat alone or in combination with phenol were found to be co ... | 1983 | 6873478 |
| observations on the texas star-sr avirulent a-b+ mutant candidate cholera vaccine. | comprehensive controlled vaccine trials have revealed that neither conventional cholera vaccines nor cholera toxoid administered parenterally are effective in inducing durable immunity against cholera. the only truly effective means of immunization against cholera as yet demonstrated conclusively only in volunteers, are (a) recovery from the disease itself and (b) that observed following oral administration of living attenuated mutants. in both instances, the host is presented with a consortium ... | 1983 | 6873480 |
| successful colonization and immunization of adult rabbits by oral inoculation with vibrio cholerae o1. | adult rabbits were inoculated orally (or duodenally) with virulent vibrio cholerae o1. jejunal colonization occurred only when hypoperistalsis was induced at the time of inoculation by tincture of opium given intraperitoneally (or by temporary ileal obstruction). for oral inoculation, prior neutralization of gastric acid was also required. inoculation with 10(9) v. cholerae caused jejunal colonization for 1 to 2 days and ileal colonization for 5 to 6 days. the extent of small bowel colonization ... | 1983 | 6874074 |
| numerical taxonomy of vibrio cholerae and related species isolated from areas that are endemic and nonendemic for cholera. | a total of 165 strains of vibrios isolated from clinical and environmental sources in the united states, india, and bangladesh, 11 reference cultures, and 4 duplicated cultures were compared in a numerical taxonomic study using 83 unit characters. similarity between strains was computed by using the simple matching coefficient and the jaccard coefficient. strains were clustered by unweighted average linkage and single linkage algorithms. all methods gave similar cluster compositions. the estimat ... | 1983 | 6874901 |
| repair of ultraviolet light-induced dna damage in cholera bacteriophages. | dna repair-proficient and -deficient strains of vibrio cholerae were used to examine host cell reactivation, weigle reactivation and photoreactivation of u.v.-irradiated cholera bacteriophages. u.v. light-induced dna damage in phages of different morphological and serological groups could be efficiently photoreactivated. host cell reactivation of irradiated phages of different groups was different on the same indicator host. phage phi 149 was the most sensitive, and phi 138 the most resistant to ... | 1983 | 6875517 |
| augmented delayed footpad reaction in thymus cell-depleted mice induced by cholera toxoid. | one hundred microgram of cholera toxoid was injected intravenously into ddd and akr mice and its effects on lymphoid tissues and immune responses against sheep erythrocytes (srbc) were examined at various times after the injection. (1) a remarkable reduction of thymus cells was revealed from day 1 to 7 and from day 1 to 4 in ddd and akr mice, respectively. (2) cholera toxoid exhibited only slight effects on the numbers of spleen cells and peripheral blood leukocytes in both strains. (3) delayed ... | 1983 | 6876141 |
| cholera on a gulf coast oil rig. | a single case of severe diarrhea on a floating texas oil rig was followed two days later by what proved to be the largest outbreak of cholera in the united states in over a century. after isolation of toxigenic vibrio cholerae el tor inaba of the typical united states phage type from the index patient's stool, the ensuing investigation detected 14 additional cases of cholera and one asymptomatic infection serologically. infection was associated with eating rice on the oil rig on a particular day ... | 1983 | 6877323 |
| non-01 vibrio cholerae gastroenteritis in new hampshire: a case report. | non-01 vibrio cholerae gastroenteritis has been reported in the literature in association with foreign travel, or seafood ingestion, particularly raw shellfish harvested from warm-water climates. non-01 v. cholerae gastroenteritis has not been reported before from new england. the case presented describes a previously healthy female who developed non-01 v. cholerae gastroenteritis approximately 26 hours after ingestion of raw cherrystone clams. | 1983 | 6881192 |
| persistence of cholera in the united states. | in 1973, 1978, and 1981, cases of cholera were acquired along the gulf coast of the united states. the isolates from all of the cases were toxigenic vibrio cholerae o-group 1, biotype el tor, serotype inaba, hemolytic, and of the same phage sensitivity pattern, and all had the same restriction endonuclease pattern by molecular genetic analysis. the strain from one of the two 1981 cases differed from the others in having a small plasmid and a negative voges-proskauer reaction. multiple importatio ... | 1983 | 6881430 |
| [antibiotic sensitivity of el tor vibrios isolated from open reservoirs]. | sensitivity of 500 el tor vibrio strains isolated from open water reservoirs was studied with respect to 14 antibiotics. some of the strains markedly differing from the other isolates by the sensitivity levels and the set of the resistance determinants were subjected to a detailed analysis. the problem of distribution of antibiotic resistant strains of the cholera germ is discussed. | 1983 | 6881946 |
| binding of choleragen and anti-ganglioside antibodies to gangliosides incorporated into preformed liposomes. | exogenously added gangliosides were taken up and incorporated into liposomes just as they are incorporated into cells. ganglioside gm1 was rapidly taken up by liposomes containing dimyristoyl- or dipalmitoylphosphatidylcholine, cholesterol and dicetyl phosphate. when incubated with a wide range of gm1 concentrations for 18 h, the liposomes incorporated about 10% of the added ganglioside. the rate of gm1 uptake by preformed liposomes was both time- and temperature-dependent. the liposomes also in ... | 1983 | 6882761 |
| colonization of the rabbit small intestine by clinical and environmental isolates of non-o1 vibrio cholerae and vibrio mimicus. | we examined the capability of 12 isolates of non-cholera toxin-producing o1 and non-o1 vibrio cholerae to colonize the small intestine of adult rabbits and cause diarrhea. using the removable intestinal tie-adult rabbit diarrhea model, we found that eight environmental isolates that showed no or marginal biological activity in other diarrhea models (rabbit ileal loop, infant rabbit, and suckling mouse) appeared to be incapable of attaching to and colonizing, even transiently, the small intestina ... | 1983 | 6885157 |
| epidemiological features of an outbreak of gastroenteritis/cholera in katsina, northern nigeria. | in april 1982, katsina, in northern nigeria, was affected by an outbreak of gastroenteritis associated with vibrio cholerae serotype 'ogawa' and 662 patients were admitted to the katsina general hospital during a 16-week period. the outbreak affected all ages and both sexes and all parts of the town and its immediate surroundings except the government residential area (gra). the overall case fatality rate was 7.7%. male specific case fatality and female specific case fatality rates were 9.7 and ... | 1983 | 6886407 |
| [ige antibody production by vibrio cholerae and its cellular components]. | 1983 | 6887516 | |
| vibrio cholerae bacteraemia in a newborn infant. a case report. | a 6-day-old black male infant presented with diarrhoea and biochemical evidence of severe electrolyte imbalance. despite treatment with intravenous fluids and antibiotics, he died within 24 hours of admission. enterotoxigenic vibrio cholerae, biotype e1 tor, serotype inaba, was isolated from the blood. the significance of this finding is discussed. | 1983 | 6612541 |
| the effect of neuraminidase on the relative surface charge-associated properties of rat red blood cells of different ages. | approx. 70% of the sialic acid on the rat erythrocyte surface is susceptible to cleavage by neuraminidase (vibrio cholerae). neuraminidase treatment results in a reduction in the partition coefficient (k) of the red cells in a charged dextran-poly(ethylene glycol) aqueous phase system and in the electrophoretic mobility of the cells. countercurrent distribution of rat neuraminidase-treated erythrocytes, containing 59fe-labeled mature red cells of distinct age, indicates that (a) the electrophore ... | 1983 | 6615837 |
| synthesis of phage-specific transfer rna molecules by vibriophage phi 149. | 32p-labelled trna was isolated from uninfected and phage phi 149-infected vibrio cholerae cells. these trna preparations were then hybridised with dna isolated from phage phi 149. significant hybridisation was observed only with trna from phage phi 149-infected cells. this strongly suggests that infection of classical vibrio with phage phi 149 results in the synthesis of phage-specific trna molecules. | 1983 | 6617890 |
| molecular epidemiological studies of united states gulf coast vibrio cholerae strains: integration site of mutator vibriophage vca-3. | environmental and clinical vibrio cholerae o-1 strains isolated from the u.s. gulf coast region were found to be lysogenic for a vibriophage which we have designated vca-3. comparison of vca-3 with the previously described vibriophages vca-1 and vca-2 has shown that vca-1 and vca-3 are homoimmune, have extensive sequence homology, but have markedly different restriction endonuclease digestion patterns. vca-3 was found to randomly integrate into the v. cholerae rv79 chromosome and to introduce st ... | 1983 | 6618665 |
| determination of serum bactericidal activity with the aid of luminous bacteria. | nonmarine luminous bacteria belonging to the genus vibrio cholerae were extremely sensitive to the bactericidal activity of human serum. luminous bacteria incubated in a medium containing serum showed a decrease in their in vivo luminescence that was directly proportional to the decrease in the viable count and was a function of the serum concentration. both immunoglobulins and the complement system were required to exert the serum bactericidal activity. serum lacking immunoglobulins or certain ... | 1983 | 6619281 |
| biological consequences of neuraminidase deficiency in newcastle disease virus. | a second-step revertant (l1) of a temperature-sensitive mutant (c1) of newcastle disease virus agglutinated erythrocytes normally but had less than 3% of the wild-type (strain av) levels of neuraminidase activity. revertant l1 had seven times more virion-associated n-acetylneuraminic acid (nana) than strain av. nana residues on purified virions were specifically labeled with periodate and tritiated borohydride. analyses of radiolabeled l1 virions on sodium dodecyl sulfate-polyacrylamide gels sho ... | 1983 | 6620458 |
| [adhesive properties of vibrio cholerae strains and mutants with varying biological characteristics]. | v. cholerae strains and mutants devoid of adhesive activity and possessing very high adhesive activity were found in the collection under study. as a rule, cholerigenic strains possessed high or moderate adhesive activity, while in noncholerigenic strains the coefficient of adhesiveness varied over a wide range. the existence of a definite pronounced correlation between lecithinase activity and adhesiveness was established. hemolysin-producing strains were found to possess high adhesive activity ... | 1983 | 6624312 |
| spectrum of vibrio infections in a gulf coast community. | infection with vibrio species was diagnosed in 23 patients over a 10-year period in a gulf coast community. species isolated were vibrio vulnificus, v. cholerae, v. parahaemolyticus, and v. alginolyticus. clinical presentations included diarrhea, wound infection, and bacteremia. gastrointestinal tract infection was infrequently diagnosed, despite the routine use of stool culture media appropriate for vibrio isolation. bacteremic infections were severe and occurred only in patients with underlyin ... | 1983 | 6625378 |
| removal of o-acetylated sialic acids from rat colonic epithelial glycoproteins by cell-free extracts of rat faeces. | sterile, cell-free, extracts of freshly defaecated wistar rat faeces in a ph 7.0 "minimal medium" contain neuraminidase(s), capable of removing sialic acids both with and without side-chain substituents from bovine submandibular mucin and rat colonic epithelial glycoproteins, and an esterase which removes o-acetyl substituents from the side chain of sialic acid residues. studies of the removal of sialic acids from bovine submandibular mucin and rat colonic epithelial glycoproteins indicated that ... | 1983 | 6627098 |
| duplication and amplification of toxin genes in vibrio cholerae. | vibrio cholerae strains of the classical biotype all contain two widely separated copies of the cholera toxin operon ctxab. in contrast, ei tor strains containing multiple copies of ctx have their copies arranged on large tandem repeats which are either 7 or 9.7 kb in length. the variation in size among these large tandem duplications was due to a difference in the copy number of a smaller, 2.7 kb, tandemly repeated sequence (rs1) that is located at the novel joint of these duplications, as well ... | 1983 | 6627396 |