Publications

[kinetics and mechanism of nucleophilic effect on the oxidation of o-dianisidine catalyzed by horseradish peroxidase].	the effects of benzimidazole and 4-nitroimidazole on the reaction of o-dianisidine peroxidase oxidation within the ph range of 3.7--9.0 were studied. both substituted imidazoles activate the reaction at less than 0.6. in the presence of 4-nitroimidazole the activation is non-competitive, whereas in the presence of benzimidazole it is of a mixed type, which is close to the non-competitive one. the kinetic parameters (kacat, alpha, ka) for the reaction activated by both imidazoles were determined. ...	1978	27245
mechanism of n-demethylation of aminopyrine by hydrogen peroxide catalyzed by horseradish peroxidase, metmyoglobin, and protohemin.		1978	667019
an autoradiographic study of midbrain-diencephalic projections to the inferior olivary nucleus in the opossum (didelphis virginiana).	techniques of intra-axonal transport were utlizied to elucidate the organization of diencephalic and midbrain projections to the inferior olivary nucleus of the virginia opossum. retrograde transport of horseradish peroxidase injected into the olive suggests that terminals within it arise from the subparafascicular nucleus of the caudal thalamus, the nucleus of darkschewitsch, the fields of forel, the interstitial nucleus of cajal, the periaqueductal grey, the caudal pretectal nucleus, the tegme ...	1978	641221
the origin of brainstem-spinal pathways in the north american opossum (didelphis virginiana). studies using the horseradish peroxidase method.		1978	8980723
[chemical modification of epsilon-nh2 groups of lysine residues in horseradish peroxidase. accessibility of these groups to different modifying agents].	modification of epsilon-nh2 groups of lysine residues in horseradish peroxidase by acetic, propionic, butyric, valeric and succinic anhydrides and trinitrobenzenesulfonic acid (tnbs) is carried out. all the anhydrides modify at 0 degrees c four epsilon-nh2 lysine groups, and tnbs--three groups. tnbs modifies all 6 peroxidase epsilon-nh2 groups at 40 degrees c. possible spacing of lysine epsilon-nh2 groups in the enzyme molecule is discussed.	1978	656503
identification of the trochlear motoneurons by retrograde transport of horseradish peroxidase.		1978	648617
the organization of central auditory pathways in a reptile, iguana iguana.	the present experiments were designed to trace the central auditory pathways in an extant reptile, the new worlkd lizard--iguana iguana, utilizing anterograde axonal degeneration stained by the fink-heimer ('67) method and the retrograde axonal transport of horseradish peroxidase (lavail and lavail, '74). beginning with the projections of the auditory portion of the viiith nerve, the ascending pathways were traced through successive relay nuclei to the telencephalon. the auditory portion of the ...	1978	632382
abnormal transport of horseradish peroxidase from the medial gastrocnemius muscle in dystrophic mice [proceedings].		1978	650592
[immunoelectron microscopy in dermatology].	the principle of immunoelectronmicroscopic studies using horseradish perpoxidase is described. this method, especially the peroxidase-antiperoxidase multistep technique, reveals more details about the exact localization of immunophenomena in different dermatological diseases. the results of immunological investigations performed on the ultra-structural level in bullous diseases, lupus erythermatosus, vasculitis, and psoriasis are summarized and compared with the immunofluorescent and classical e ...	1978	348650
uptake of horseradish peroxydase by the testis of locusta migratoria during the last larval instar; relation with variations of ecdysteroid levels in haemolymph.	by using horseradish peroxydase (hrp) as a tracer, it is shown that the gonial region of the locust testis is an "open" compartment which is almost always freely penetrated by the tracer. during the last larval instar, however, the penetration of hrp decreases and ceases at the time when high levels of ecdysteroids are detected in the haemolymph by radioimmunoassay. a cause and effect relationship between tracer uptake and hormonal level could not be demonstrated by the experiments carried out u ...	1978	639097
kinetic analysis of the acid-alkaline conversion of horseradish peroxidases.	the nature of the acid-alkaline conversion of horseradish peroxidases was studied by measuring four rate constants in reactions, e + h+ (k1) in equilibrium (k2) eh+ and e + h2o (k3) in equilibrium (k4) eh+ + oh-, where eh+ and e denote the acid and alkaline forms of the enzymes. the values of k1, (k2 + k3), and k4 were obtained by measuring the relaxation rates of the acid leads to alkaline and alkaline leads to acid conversions by means of th ph jump method with a stopped-flow apparatus. the va ...	1978	24465
proteolytic and peroxidatic reactions of commercial horseradish peroxidase with myelin basic protein.	degradation of myelin basic protein during incubations with high concentrations of horseradish peroxidase has been demonstrated [johnson & cammer (1977) j. histochem. cytochem.25, 329-336]. possible mechanisms for the interaction of the basic protein with peroxidase were investigated in the present study. because the peroxidase samples previously observed to degrade basic protein were mixtures of isoenzymes, commercial preparations of the separated isoenzymes were tested, and all three degraded ...	1978	77159
interaction of the hemin 2 and 4 substituents with apo horseradish peroxidase.		1978	637861
calcium-related properties of horseradish peroxidase.		1978	416823
localization of rabbit's flocculus purkinje cells projecting to the cerebellar lateral nucleus and the nucleus prepositus hypoglossi investigated by means of the horseradish peroxidase retrograde axonal transport.		1978	19605112
neural spread of herpes simplex virus types 1 and 2 in mice after corneal or subcutaneous (footpad) inoculation.	twelve herpes simplex virus (hsv) strains, 6 of each type, were inoculated subcutaneously into the left hind foot and into the cornea of the right eye of 12-day-old swiss albino mice. the neural spread of virus to trigeminal and spinal ganglia and to brain and spinal cord was studied by demonstration of infective virus, histology and electron microscopy. type 1 and type 2 infections seemed to spread equally well by intra-axonal transport. using a protein tracer (horseradish peroxidase) injected ...	1978	632838
ionic strength dependence of the oxidation of iodide and ferrocyanide by compound i of horseradish peroxidase.	the kinetics of the oxidation of iodide and ferrocyanide by compound i of horseradish peroxidase have been studied at 25 degrees c as a function of ionic strength and ph. the ionic strength dependencies of the second-order rate constants are tested with an extended form of the debye-hückel equation for the activity coefficients of the reacting species. for the reaction of iodide with compound i it is shown that the ph variation of the rate constant at zero ionic strength is caused mainly by titr ...	1978	624287
lignin synthesis: the generation of hydrogen peroxide and superoxide by horseradish peroxidase and its stimulation by manganese (ii) and phenols.	the enzyme horseradish peroxidase (ec 1.11.1.7) catalyses oxidation of nadh. nadh oxidation is prevented by addition of the enzyme superoxide dismutase (ec 1.15.1.1) to the reaction mixture before adding peroxidase but addition of dismutase after peroxidase has little inhibitory effect. catalase (ec 1.11.1.6) inhibits peroxidase-catalysed nadh oxidation when added at any time during the reaction. apparently the peroxidase uses hydrogen peroxide (h2o2) generated by non-enzymic breakdown of nadh t ...	1978	24414365
the bound auxins: protection of indole-3-acetic acid from peroxidase-catalyzed oxidation.	indole-3-acetic acid (iaa) was oxidized by horseradish peroxidase, but ester and amide conjugates of iaa were not degraded. addition of indoleacetyl-myo-inositol, indoleacetyl-l-aspartate, indoleacetylglycine, indoleacetyl-l-alanine, indoleacetyl-d-alanine, or indoleacetyl-β-alanine did not affect the rate of oxidation of iaa by horseradish peroxidase. peroxidase preparations from pisum sativum l. and zea mays l. behaved similarly in that they rapidly oxidized iaa, but not conjugates found in th ...	1978	24414261
cytochemical localization of exogenous peroxidase in adrenal medullary cells of hamster.	the endocytosis in hamster adrenal medullary cells has been studied by intravenously administered horseradish peroxidase, which was then detected with the graham-karnovsky method. the protein uptake was investigated 30, 60, and 120 min after the peroxidase injection. the exogenous protein was accumulated mostly in the lumen of the sinusoids, around the capillaries as well as at the apical pode of chromaffin reaction product was detectable also in the chromaffin cells. the protein uptake was very ...	1978	754419
efferent connections of the septal area in the pigeon.	the efferent connections of the septum in the pigeon were studied with the use of anterograde (3h-leucine autoradiography) and retrograde (horseradish peroxidase histochemistry) transport methods. it was observed that the dorsal septum projects to the lateral hypothalamus, portions of the periventricular hypothalamus, dorsomedial thalamus and midbrain reticular formation. fibers from the ventral half of the septum (i.e., nucleus of the diagonal band) project to medial and lateral sectors of the ...	1978	737528
equilibria between horseradish peroxidase and aromatic donors.	equilibria between horseradish peroxidase and aromatic hydrogen donors have been analyzed spectrophotometrically and potentiometrically. the donors alter the peroxidase spectrum slightly but reproducibly with changes of two types. donors of the two groups compete for the same binding site with no systematic difference in affinity for the enzyme. donors with one aromatic ring are fairly loosely ligated, kd3-25 mm, but enlargement, or extension of the pi-electron system, increases the affinity. a ...	1978	706949
the influence of taipoxin on cholinergic synapses in the electric organ of torpedo marmorata.	the influences of the snake venom taipoxin on the cholinergic synapses of the electric organ of torpedo mamorata were investigated. 1. in ultrathin sections presynaptic membrane indentations are noted under the influence of taipoxin/nacl. 2. the presynaptic membranes often show small interruptions. 3. in taipoxin/horseradish peroxidase incubated organ pieces typical omega-shaped indentations are found, but on the postsynaptic side. 4. the vesicle density decreased under the influence of the taip ...	1978	681922
[anomalously high pkapp shifts in protoporphyrin ix protonization during its solubilization on the apoperoxidase from horseradish and on surfactant micelles. the effect of a nonpolar environment on the pkaff shift].		1978	679814
[electrophysiologic and anatomic demonstration of inhibitory commisural vestibular neurons in the tench (tinca tinca)].	horseradish peroxydase was injected intracellularly in some of the medullary neurons which, in fish, exhibit a passive hyperpolarizing potential after spinal cord stimulation (this indicates that such neurons both electrical and chemical inhibitions of the mauthner cells). with positive staining, commissural vestibular neurons were identified; their axons cross the midline and they establish connexions with the mauthner cell and with vestibular neurons of both sides. thus the existence of a cros ...	1978	417859
distribution of neurons projecting to the retina of caiman crocodilus.	horseradish peroxidase (hrp) was injected into the vitreous of the eye, the orbital cavity, or the optic tectum of caiman crocodilus. following intravitreal injections, retrograde transport of the enzyme was observed bilaterally, but predominantly contralaterally, in a large oblong field of cells at the isthmic level of the midbrain, bounded medially by the trochlear nucleus and laterally by the nucleus isthmi. control injections of hrp into the orbital cavity and eye muscles labelled motoneuron ...	1978	81087
analysis of acid-base properties of peroxidase and myoglobin.	two heme-linked groups of horseradish peroxidases, characterized by pka = 5.8 (isoenzyme a) and 7.3 (isoenzyme c), are believed to be distal amino acid residues and, judging by their acid-base properties in various derivatives, influence the functions of these isoenzymes. in contrast, in myoglobin ionization of the distal histidine does not influence its reactivity. accordingly, we conclude that the interaction of the distal base with a 6th ligand is weak in myoglobin but very strong in peroxida ...	1978	27955
nuclear magnetic resonance studies of high-spin ferric hemoproteins.	220 mhz proton fourier transform (ft) nmr with quadrature phase detection (qpd) technique is applied to observe largely hyperfine-shifted signals of various hemoproteins and hemoenzymes in ferric high-spin state. the binding of f-, ocn-, scn-, and ch3oh to the ferric heme iron in high-spin state in various hemoproteins has been studied by the use of ft/qpd technique at 220 mhz. the binding of formate ion to metmyoglobin (metmb) has also been studied. the spectrum of the formate complex was compa ...	1978	27954
[effect of n-alkylimidazoles on oxidation of o-dianizidine catalyzed by horseradish peroxidase].	effect of a number of n-alkylimidazoles (from n-methyl to n-octylimidazole) on peroxidase oxidation of o-dianizidine at ph 8.0 is studied. alkylimidazoles studied are found to activate the reaction under given conditions, the activation type being non-competitive ka and (alpha) values are similar for all the activators, which suggests a similar mechanism of their action. similar ka values suppose an insignificant role of hydrophobic interactions in the binding of n-alkylimidazoles with the enzym ...	1978	26428
location of single neurons containing hrp for electron microscopy.	a technique is described for finding single neurons containing transported horseradish peroxidase (hrp) for study in the electron microscope. tissue chopper sections which have been reacted to demonstrate hrp are cleared in glycerol and examined with darkfield light microscopy. the granular reaction product is clearly seen, enabling accurate drawings to be made for later dissection for ultramicrotomy. the neuron sought can then be found within a few microns of the cutting face of the block. whil ...	1977	75048
vagal preganglionic innervation of the cat heart--an attempt to identify the medullary "heart area" by the retrograde axoplasmic transport of horseradish peroxidase.	the medullary "heart area", origins of the vagal preganglionic fibers innervating the heart, were identified in 19 cats with the use of horseradish peroxidase (hrp) as a retrograde axonal tracer. when hrp was injected in the a-v node area, s-a node area, right atrial wall and right ventricular wall, the labeled neurons were principally found in the lateral half of the dorsal motor nucleus (dmn) and in the medial solitary nucleus (msn), were almost the same in number bilaterally, and were chiefly ...	1977	75272
noradrenaline innervation of the spinal cord studied by the horseradish peroxidase method combined with monoamine oxidase staining.	the origin of the spinal cord noradrenaline (na) has been investigated by means of the horseradish peroxidase (hrp) method, combined with monoamine oxidase staining (glenner) to identify the na neurons. following the injection of hrp to the various levels of rat spinal cord, cervical to sacral cord, a1-3, 5-7 na neuron groups were labeled with hrp. they showed almost the same distribution pattern regardless of difference in the injected segment. labeled na neurons in a6 were concentrated in the ...	1977	74341
the pontine projection to the flocculonodular lobe and the paraflocculus studied by means of retrograde axonal transport of horseradish peroxidase in the rabbit.	the occurrence and distribution of labeled cells in the pontine nuclei were mapped following injections of small amounts of horseradish peroxidase (0.05-0.5 microliter, 50% suspension) in the flocculus, nodulus and the dorsal and ventral paraflocculus in adult albino rabbits. while no labeled cells were found in the pontine nuclei following injections in the nodulus, some were present following injections in the flocculus and a great number following injections in the paraflocculus. the projecti ...	1977	74343
further studies on hybrid cell-surface antigens associated with human chromosome 11.	a new human immunogenetic cell-surface activity associated with human chromosome 11 in the al human-chinese hamster ovary cell hybrid is described. like a1, but not a2, it is present on the human erythrocyte. by mutagenesis and selection, specific, stable, variants of the al hybrid have been prepared exhibiting various combinations of a1, a2, a3, and lactic dehydrogenase a activities. the antigens of the al system can be demonstrated by the horseradish peroxidase system which offers a promising ...	1977	74861
techniques for histological reconstruction of brain stem recording sites.	the masseter muscle of sprague-dawley rats was injected with 10 mg of horseradish peroxidase. the electrical activity of the trigeminal motor nucleus was investigated 24-36 hours later using micropipettes filled with a 2.0 m nacl-7% fast green fcf solution. a parallel series of penetrations at 200 micron intervals was made through the motor complex at known depths. the grid formed from these penetrations was reconstructed using the following techniques. marks were made at known depths in one or ...	1977	74873
descending course of primary afferent collaterals in the cat's spinal cord.	after injecting horseradish-peroxidase into the lower thoracic, lumbar and sacral spinal cord segments of cats, labelled perikarya were found in several spinal ganglia localized cranially to the site of injection. the segmental distance between the injection site and the rostralmost localized ganglion with labelled cells varied depending on the medio-lateral localization of the injection. the longest distance (10 segments) was achieved by medial injections. primary sensory neurones with long des ...	1977	79302
intraaxonal transport of horseradish peroxidase in the sympathetic nervous system.	following a single injection of horseradish peroxidase (hrp) into the superior cervical ganglion (scg) of the rabbit, the uptake and anterograde transport of this label was confirmed in the ganglion cell bodies, postganglionic axons, and preterminal and terminal ending axons in the ciliary processes of the eye. from the same injection site the intraaxonal hrp reaction product was demonstrated in myelinated axons, presumably by retrograde transport. intracytoplasmic hrp was identified in large, s ...	1977	64283
identification of cells or origin of non-primary afferents to the dorsal column nuclei of the cat.	in order to identify cells of origin in the spinal cord of non-primary afferents to the dorsal column nuclei (dcn), the retrograde transport of horseradish peroxidase (hrp) has been utilized in adult cats. 10 to 30% hrp was injected bilaterally (0.6 mul per side) in the dorsal medulla of nine cats. in most instances the spread of the injected enzyme extended a few millimeters rostrocaudally and infiltrated the dcn as well as other nuclei and fiber tracts. labelled cells in these cases are numero ...	1977	64365
anatomical studies of a temporal visual area in the rabbit.	using fink-heimer, autoradiographic and horseradish peroxidase techniques, the connections of a temporal visual cortical region of the rabbit were explored. the temporal visual area covers portions of areas t1 and t2, and is reciprocally connected with the posterior nucleus and suprageniculate nuclei of the thalamus. it was also shown that the temporal visual area projects to a similar region in the opposite hemisphere, and to intermediate laminae of the superior colliculus. the temporal visual ...	1977	64476
functional organization of the vestibular input to the anterior and posterior cerebellar vermis of cat.	1. responses evoked by electrical stimulation (auditory division of the viiith nerve sectioned chronically) and natural stimulation of the vestibular apparatus were recorded in the anterior and posterior cerebellar vermis of cats anesthetized with ketamine or nembutal. under ketamine the functional state of the cerebellar cortex was similar to that of the decerebrate or encéphale isolé preparation. 2. vestibular-evoked responses were found bilaterally throughout the vermis (lob. i-x) and parts o ...	1977	65291
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. iv. the projection to the anterior lobe.	following injections of horseradish peroxidase (hrp) in the cerebellar cortex of the anterior lobe of the cat, the distribution of labeled cells in the inferior olive was mapped. the findings largely confirm those made previously in studies of olivary retrograde cell loss following cerebellar ablations (brodal, '40b). in addition, they reveal further olivary areas projecting onto the anterior lobe, and permit a more detailed analysis of the pattern in this projection. concerning major points the ...	1977	65365
brain stem afferents to the fastigial nucleus in the cat demonstrated by transport of horseradish peroxidase.	although retrograde and anterograde degeneration studies have provided important information concerning brain stem afferents to the fastigal nucleus (fn), these data may be incomplete and should be confirmed by axonal transport methods. attempts were made to inject horseradish peroxidase (hrp) unilaterally into the fn in a series of adult cats. animals were perfused with dextran and a fixative solution of paraformaldehyde and glutaraldehyde in 0.1 m phospate buffer. representative sections were ...	1977	65366
the vestibular complex of the american opossum didelphis virginiana. ii. afferent and efferent connections.	we have demonstrated the connectivity of the opossum's vestibular nuclei using degeneration, autoradiographic and horseradish peroxidase techniques and have found it to be generally comparable to that reported for the cat. apart from the primary input described in part i of our study, the cerebellum provides the major source of afferent connections to the vestibular complex. axons from the cerebellar cortex distribute mainly to vestibular areas which receive no primary afferent projections, e.g. ...	1977	65367
ultrastructural visualization of cellular carbohydrate components by means of lectins on ultrathin glycol methacrylate sections.	a method for the visualization of cellular carbohydrate components by both light and electron microscopy using lectins on glycol methacrylate sections is proposed. this method, which is an application of the lectin-peroxidase affinity technique, solves the problem of limited penetration when it is attempted to demonstrated lectins receptors within the tissue block. following partial dissolution of glycol methacrylate from thin sections using alcohol, they are incubated successively with lectin ( ...	1977	65417
organization of the facial nerve nucleus in the cat.	the location of efferent neurons in the brain stem of newborn kittens supplying the facial muscles was studied by means of horseradish peroxidase (hrp) used as a tracer of retrograde protein transport. three to 15 microliters containing 0.3 to 1.5 mg of hrp was injected into the orbicularis oris and orbicularis oculi muscles of six kittens and in the seventh kitten, the ventral ramus of the facial nerve was transected and hrp was instilled on the cut end of the nerve. after a survival time of 24 ...	1977	65707
brain stem projections to lobule vii of the posterior vermis in the squirrel monkey: as demonstrated by the retrograde axonal transport of tritiated horseradish peroxidase.		1977	66085
localization of abductor and adductor fibers in the kitten recurrent laryngeal nerve: use of a variation of the horseradish peroxidase tracer technique.		1977	66152
retrograde axonal transport of horseradish perioxidase in afferent fibers of the inferior mesenteric ganglion of the guinea pig. identification of the cells of origin in dorsal root ganglia.		1977	66970
orthograde and retrograde axoplasmic transport during acute ocular hypertension in the monkey.	orthograde and retrograde axoplasmic transport have been studied in the optic nerve heads of 37 macaca fascicularis eyes with normal or elevated intraocular pressure (iop) produced by cannulation of the anterior chamber. orthograde transport was labeled by 3h-amino acids injected intravitreally and incorporated into retinal ganglion cell proteins. retrograde transport was studied in the same eyes by injecting horseradish peroxidase (hrp) into one or both optic tracts and dorsal lateral geniculat ...	1977	67096
identification of different size motoneurons labeled by the retrograde axonal transport of horseradish peroxidase.	three main groups of motoneurons of different size have been labeled in adult cats by using the method for retrograde axonal transport following injection of horseradish peroxidase in the medial gastrocnemius and soleus muscles. in particular small, medium-size and large neurons which probably correspond respectively to gamma, small alpha and large alpha motoneurons innervating the calf muscles, have been identified and the corresponding area measured.	1977	67590
the organization of thalamocortical relay neurons in the rat ventrobasal complex studied by the retrograde transport of horseradish peroxidase.		1977	68039
commercial horseradish peroxidase degrades myelin encephalitogenic protein during coupling for immunohistochemical studies.	conjugates of myelin encephalitogenic basic protein (ep) and commercial horseradish peroxidase (hrp) have been used for immunohistochemical demonstrations of anti-ep antibody in animals with experimental allergic encephalomyelitis. we performed gel electrophoresis studies on ep-hrp conjugates prepared with glutaraldehyde and on mixtures of ep and hrp incubated without glutaraldehyde. the results show that under conditions of one-and two-step coupling hrp causes rapid loss of the native ep band, ...	1977	68067
[study of the vagal innervation of the cat stomach by means of retrograde axoplasmic transport of horseradish peroxidase--on the localization of the origins of the preganglionic fibers].		1977	68782
retrograde axonal transport of horseradish peroxidase. uptake at mouse neuromuscular junctions following systemic injection.	eye muscles were examined histochemically for the presence of horseradish peroxidase (hrp) activity after intravenous injections of large doses of this tracer protein. hrp had penetrated through the blood vessels and diffused into the areas of motor end plates, where it outlined axon terminals at the synaptic clefts. hrp was incorporated into pinocytotic vesicles in the axons from where an intraaxonal transport in the retrograde direction to the nerve cell bodies in the brainstem followed. accum ...	1977	69382
demonstration of bilateral claustro-cortical connections in the cat with the method of retrograde axonal transport of horseradish peroxidase.	the claustrum of the cat was studied to determine the extent of its projection to the cerebral cortex. neurons in the bilateral dorsal claustrum are labeled by retrograde axonal transport of horseradish peroxidase (hrp) from injection sites of different neocortical areas with preponderance to the ipsilateral side. it is revealed that the dorsal claustrum projects to almost entire regions of the bilateral neocortex and that distribution of labeled neurons in the bilateral claustrum has topographi ...	1977	69423
identification of cells of origin of tectopontine fibers in the cat superior colliculus: an experimental study with the horseradish peroxidase method.	the pontine projections from the superior colliculus in the cat have been studied by means of retrograde axonal transport of horseradish peroxidase (hrp). following injections of hrp in the dorsolateral pontine nucleus, where the tectopontine fibers terminate, a fair number of labeled cells are found throughout the rostrocaudal extent of the ipsilateral superior colliculus. relatively few of the labeled cells are of medium size (25-40 micron in diameter), more than 80% are small (10-25 micron), ...	1977	69477
the effects of monocular deprivation on different neuronal classes in the lateral geniculate nucleus of the cat.	retrograde axonal transport of horseradish peroxidase (hrp) was used to identify two populations of cells in the lateral geniculate nucleus (lgn) of the cat. hrp was injected into area 17 and 18 separately in the same animals, and the neuronal somata giving rise to thalamo-cortical axons, identified by the presence of granular hrp reaction product within them, were measured. the mean size of lgn neurones labelled by injections in area 17 ("17-relay" cells) was less than of neurones filled from a ...	1977	69546
ultrastructural immunoenzymatic study of alpha-fetoprotein-producing cells in the human fetal liver.	cells producing alpha-fetoprotein in human fetal liver have been studied with specific horseradish peroxidase labeled immunoglobulins. under light microscopy, the alpha-fetoprotein is strictly localized in the cytoplasm of certain hepatocytes, distributed randomly in the hepatic lobule. ultrastructural examination of thesame cells shows that the alpha-fetoprotein is present within the cytoplasm. ultrastructural differences are described in hepatocytes according to whether or not the cell is prod ...	1977	69654
axoplasmic transport in ocular hypotony and papilledema in the monkey.	orthograde and retrograde axoplasmic transport were studied in optic nerve heads of seven hypotensive macaca fascicularis eyes. orthograde transport was studied by radioautography after intravitreal radioisotope injections. retrograde transport was studied in the same eyes by horseradish peroxidase injection into the dorsal lateral geniculate nuclei or optic tracts. three eyes had developed marked papilledema before injections. orthograde axoplasmic transport was blocked in swollen axons of the ...	1977	70200
bidirectional movement of horseradish peroxidase and the demonstration of reciprocal thalamocortical connections.		1977	70257
evidence for direct synaptic interconnections between cat spinal alpha-motoneurons via the recurrent axon collaterals: a morphological study using intracellular injection of horseradish peroxidase.	by using intracellular injection of horseradish peroxidase into cat triceps surae alpha-motoneurons we have been able to trace the motor axons as well as the recurrent motor axon collaterals with their synaptic terminals both with the light and electron microscope. in addition to the expected projection to the 'renshaw cell area', the triceps surae motor axon collaterals were frequently found to terminate within the motor nuclei, where at least some of the terminals made direct synaptic contact ...	1977	70258
role of gangliosides in the uptake and retrograde axonal transport of cholera and tetanus toxin as compared to nerve growth factor and wheat germ agglutinin.	previous investigations have shown that tetanus toxin is transported retrogradely in all peripheral neurons whereas the transport of ngf is confined to adrenergic and sensory neurons. other macromolecules with molecular weights and general physiochemical properties similar to ngf and tetanus toxin (e.g., cytochrome c, insulin, horseradish peroxidase and bovine serum albumin) are not transported to a detectable extent if injected in comparable molar concentrations. for tetanus toxin, which is tra ...	1977	70259
retrograde axonal transport of horseradish peroxidase for determining motor projection patterns to the developing limb in xenopus.	horseradish peroxidase (hrp) injected into developing limb buds of xenopus laevis tadpoles is carried by retrograde axonal transport to the somata of motoneurones in the ventral horn. small injection of 10% hrp were found to remain well localised to specified sites in the limb bud. two types of labelled cells were found: diffusely labelled and granular labelled. diffusely labelled cells result from axonal damage in the presence of hrp. granular labelled cells result only from uptake of hrp from ...	1977	70260
[projections of bulbar reticular nuclei and raphe nuclei to lobules vi and vii of cat cerebellar cortex].	injections of horseradish peroxidase were made in lobules vi and vii of the cerebellar vermis (in the cat). serial frontal sections of the brain stem reveal that the lateral and paramedian nuclei were essentially projected onto lobule vi, and the projections from the raphe nuclei onto lobule vii.	1977	70281
origin of the direct hippocampus--anterior thalamic bundle in the rat: a combined horseradish peroxidase--golgi analysis.		1977	71242
localization of thyroiglobulin antigenicity in rat thyroid sections using antibodies labeled with peroxidase or 125i-radioiodine.	in the hope of localizing thyroglobulin within focullar cells of the thyroid gland, antibodies raised against rat thyroglobulin were labeled with the enzyme horseradish peroxidase or with (125)i-radioiodine. sections of rat thyroids fixed in glutaraldehyde and embedded in glycol methacrylate or araldite were placed in contact with the labeled antibodies. the sites of antibody binding were detected by diaminobenzidine staining in the case of peroxidase labeling, and radioautography in the case of ...	1977	71304
movement of horseradish peroxidase in the cornea, sclera and the anterior uvea.	the anterior chambers of rabbit, monkey and human eyes were perfused with horseradish peroxidase. the perfusion was started immediately after enucleation of the monkey and human eyes, while the rabbit eyes were perfused in situ after the animals had been killed. comparative results were obtained after 1 h or perfusion and were mainly based on frozen sections. intensive staining was found in the descemet's membrane, the sclera and the iridial stroma, considerably more than in the corneal stroma a ...	1977	71844
the permeability of the human ciliary and iridial epithelium to horseradish peroxidase. an in vitro study.	the permeability of the human ciliary epithelium to horseradish peroxidase (po) has been studied in vitro with the electron microscope. ciliary body and iris specimens were obtained from freshly enucleated eyes. po was applied at the stromal side of the epithelium, and was left for 120 min. the movement of po through the intercellular spaces of the human ciliary epithelium was blocked apically in the lateral intercellular spaces of the non-pigmented epithelial cells, indicating that these cells ...	1977	71845
arborization of initial axon collaterals of spinocervical tract cells stained intracellularly with horseradish peroxidase.	the initial axon collateral arborizations of two feline spinocervical tract cells have been analysed after intracellular staining with horseradish peroxidase. the images of the cells were reconstructed in the light microscope from transverse serial sections. the cell bodies were situated in laminae iv or iii of the lumbar dorsal horn. each cell exhibited one initial collateral. these emerged from the parent axons at distances of about 310 and 580 micron, respectively, from the cell body. the col ...	1977	71934
the pontine projection to the crebellar anterior lobe. an experimental study in the cat with retrograde transport of horseradish peroxidase.		1977	72000
detection of epstein-barr virus antigens with enzyme-conjugated antibody.	monospecific conjugated (fluorescein isothiocynate and horseradish peroxidase) goat antisera, prepared against three human immunoglobulin classes, im (mu), igg (gamma) and iga (alpha), were compared for their ability to detect human ig classes possessing specificity for epstein-barr virus (ebv) viral capsid antigens (vca) in a chronically infected human lymphoid cell line, p3j-hrik. it was determined that the enzyme system was significantly more sensitive than immunofluorescence in detecting mos ...	1977	72045
observations on labeling of neuronal cell bodies, axons, and terminals after injection of horseradish peroxidase into rat brain.		1977	72079
the olivocerebellar projection studied with the method of retrograde axonal transport of horseradish peroxidase. v. the projections to the flocculonodular lobe and the paraflocculus in the rabbit.	hrp was injected in the flocculonodular lobe and the paraflocculus in the rabbit to determine the areas of the inferior olive which project onto these cerebellar regions. following injections in the flocculus labeled cells occurred in the dorsal cap and the rostralmost tip of the medial accessory olive. following injections in the nodulus labeled cells were likewise found in the dorsal cap, but in addition in the rostralmost part of the dorsomedial cell column and the adjoining part of the media ...	1977	72080
the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. vi. the projection onto longitudinal zones of the paramedian lobule.	microinjections (30-50 nl) of a horseradish peroxidase (hrp) suspension of 25% (wt./vol.) were made in different folia of the paramedian lobule of cats, and the sites of occurrence of labeled cells in the inferior olive were precisely determined. in each case only a small number of cells are labeled, aggregated in a minute area. the labeled cells are found within three only of the four olivary areas previously determined (brodal et al., '75) to project onto the paramedian lobule (fig. 1): one ar ...	1977	72081
salivatory neurons innervate the submandibular and sublingual glands in the rat: horseradish peroxidase study.		1977	72589
cerebeller afferents from neurons in motor nuclei of cranial nerves demonstrated by retrograde axonal transport of horseradish peroxidase.		1977	72590
somatopetal transport of horseradish peroxidase (hrp) in the peripheral and central branches of dorsal root ganglion cells.	the axonal transport of hrp in both the peripheral and central branches of dorsal root ganglion cells was studied in rats. for studying axonal transport in the peripheral branch hrp as a dry substance was applied to the peroneal nerve injured either by teasing, by cutting or crushing. after a short survival time (22 h) mainly small spinal ganglion cells of the corresponding segments were labelled, while after a prolonged survival time (70 h) mainly large cells were labelled. these labelling diff ...	1977	72611
afferents to the entorhinal cortex of the rat studied by the method of retrograde transport of horseradish peroxidase.		1977	72679
effect of cyanide on nadph oxidation by granules from human polymorphonuclear leukocytes.	cyanide has been shown to stimulate both oxygen uptake and hexose monophosphate shunt activity in phagocytizing human polymorphonuclear leukocytes. it also stimulates the oxidation of nadph by a particulate fraction derived from phagocytizing cells. this stimulation of nadph oxidase is not observed in the presence of exogenous mn2+. studies with purified enzymes have shown that cn- also stimulates nadph oxidation by horseradish peroxidase or lactoperoxidase, suggesting that the respiratory burst ...	1977	13879
purification of bovine thyroid peroxidase.	trypsin-solubilized peroxidase activity from beef subcellular particles was resolved by deae-cellulose chromatography into 5 fractions, which contained enzymatically active components that ranged in molecular size from 73,000 to 340,000 daltons. the most active fraction (mol wt, 92,000 by gel filtration) was further purified (59,000-fold overall) by chromatography on hydroxylapatite. this highly purified peroxidase preparation had an absorbance purity ratio (a410:a280) of 0.55 and oxidized iodid ...	1977	15822
the role of cytochrome p-450 in the hydroperoxide-catalyzed oxidation of alcohols by rat-liver microsomes.	the organic hydroperoxide cumene hydroperoxide is capable of oxidizing ethanol to acetaldehyde in the presence of either catalase, purified cytochrome p-450 or rat liver microsomes. other hemoproteins like horseradish peroxidase, cytochrome c or hemoglobin were ineffective. in addition to ethanol, higher alcohols like 1-propanol, 1-butanol and 1-pentanol are also oxidized to their corresponding aldehydes to a lesser extent. other organic hydroxyperoxides will replace cumene hydroperoxide in oxid ...	1977	20305
adsorption of horseradish peroxidase, ovomucoid and anti-immunoglobulin to colloidal gold for the indirect detection of concanavalin a, wheat germ agglutinin and goat anti-human immunoglobulin g on cell surfaces at the electron microscopic level: a new method, theory and application.	a method is described for the adsorption of selected macromolecules to colloidal gold which is then used as an electron dense marker for the indirect detection of specific cell surface molecules. membrane bound concanavalin a, which binds specific sugars on horseradish peroxidase, and wheat germ agglutinin, which binds specific sugars on ovomucoid are detected indirectly with gold labeled horseradish peroxidase and ovomucoid, respectively. goat anti-human igm on blood lymphocytes is detected wit ...	1977	21217
efferent connections of the septal area in the rat: an analysis utilizing retrograde and anterograde transport methods.	experiments were performed by either retrograde (horseradish peroxidase histochemistry) or anterograde ([3h]leucine radioautography) transport methods to determine the efferent connections of the septum in the rat. it was observed that the dorsal septum projects to the lateral preoptic area, lateral hypothalamus, periventricular hypothalamus and midline thalamus. fibers from the ventral half of the septum project topographically to the hippocampal formation, thalamus, hypothalamus and midbrain. ...	1977	63306
anatomical and functional aspects of the septo-hippocampal projections.	the origins, distribution, and cellular targets of the septo-hippocampal projections are reviewed. it appears that the distribution of acetylcholinesterase-positive neurons in the medial septum and diagonal bands and those cells labelled after injections of horseradish peroxidase into the hippocampus coincide; however, the possibility of a non-acetylcholinesterase septal projection remains. good agreement is found between the distribution of hippocampal acetylcholinesterase and the patterning of ...	1977	83225
[ultrastructural demonstration of the phagocytic activity of periodontal ligament fibroblasts in the rat using enzymatic tracers].	the ultrastructural study of non-decalcified periodontal ligament of rats, labeled with horseradish peroxidase used as an enzymatic tracer for the mechanism of endocytosis, shows that there exists in the fibroblast, on the one hand, an endogeneous peroxidase revealed by the preparation and characteristic of certain cellular organelles: mitochondria, free ribosomes, ribosomes of the granular endoplasmic reticulum; and on the other hand an exogeneous peroxidase which demonstrates the ability of fi ...	1977	122704
the ultrastructural histochemistry of the basement membranes of the exocrine pancreas.	the basement membranes in the exocrine pancreas were examined by routine electron microscopy, by fixation for electron microscopy with a glutaraldehyde-tannic acid solution and by staining for ultrastructural demonstration of mucosubstances with the dialyzed iron, high iron diamine, ruthenium red and concanavalin a-horseradish peroxidase procedures. the basement membranes are considered from morphologic and histochemical observations to consist of an inner lamina lucida, an intermediate lamina ...	1977	143223
investigation of the endothelial cell changes in the brain of shr using horseradish peroxidase technique and enzyme histochemistry [proceedings].		1977	143545
hydrogen peroxide production and killing of staphylococcus aureus by human polymorphonuclear leukocytes.	the effects of bacterial neuraminidase on production of hydrogen peroxide (h2o2) and killing of staphylococcus aureus by human polymorphonuclear leukocytes (pmn) were studied. the concentration of h2o2 was measured by the disappearance of scopoletin fluorescence in the presence of horseradish peroxidase. the results indicated that desialylation of human pmn inhibited the stimulation of h2o2 production during phagocytosis. it also markedly impaired the killing of s. aureus. impaired killing of s. ...	1977	189859
relay cell classes in the lateral geniculate nucleus of the cat and the effects of visual deprivation.	this study presents evidence that the x- and y-cells described physiologically in the a laminae of the cat's dorsal lateral geniculate nucleus (lgn) are two morphologically distinct cell types recognizable in golgi preparations. it is shown firstly that the three cell types seen in golgi preparations of the a laminae (large and medium-sized principal cells and small interneurons-types 1,2 and 3 in the classification of guillery, '66) may be identified in 1-mum epon sections of osmicated material ...	1977	190274
tissue-specific antibodies against human lung and breast carcinoma dehistonized chromatins.	tissue-specific antisera against human lung and breast carcinoma dehistonized chromatins were obtained. the specificity of these antisera was determined by complement fixation. in the presence of antiserum against human lung carcinoma, only chromatins from lung carcinoma fixed complement significantly, whereas chromatins isolated from human breast carcinoma, hela cells, normal lung tissue, breast tissue, or term placenta were negative (i.e., inactive). in a similar assay with the use of antiseru ...	1977	195066
solid-phase enzyme immunoassay for immunoglobulin m antibodies to cytomegalovirus.	a sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin m antibodies to cytomegalovirus is described. the results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei ...	1977	195977
protein a-peroxidase: a valluable tool for the localization of antigens.	protein a of staphylococcus aureus has been conjugated to horseradish peroxidase and used in an indirect immunolabeling technique to visualize membrane and viral antigens. the same protein a-peroxidase conjugate was used with antisera from five different species. using this indirect test, membrane markers for t and b lymphocytes were labeled with a greater specificity than when peroxidase conjugated anti-immunoglobulin was used in the second step. viral antigens on cells infected with measles, v ...	1977	199666
the functional capacity of guinea pig megakaryocytes. ii. the uptake of particles and macromolecules and the effect of rabbit antiguinea pig platelet antiserum.	the capacity of megakaryocytes to take up particles and macromolecules was tested by exposing them to homologous erythrocytes, latex particles, micrococcus lysodeikticus, and horseradish peroxidase. uptake of particles 1.1 to 7 mum. in size was extensive under the experimental conditions employed. after uptake of each of the three particles the addition of tannic acid and calcium to the fixative produced electron-dense deposits within the vacuole surrounding the particle; therefore, the vacuole ...	1977	320386
an ultrastructure study on antibody production of the lymph nodes of rats with special reference to the role of germinal centers.	antibody-containing cells in lymph nodes, expecially in germinal centers, and in thoracic duct of rats sensitized by injection of horseradish peroxidase were studied with the immunoperoxidase method. in the primary response the large germinal center cells began to produce antibody on the 9th day and predominated thereafter in germinal centers ,added by intercellular antibody deposition in its late stage. the "ordinary" medium-sized germinal center cells were a minor component. in the secondary r ...	1977	322442
intimal healing. the pattern of reendothelialization and intimal thickening.	studies were undertaken to investigate further the basis of intimal proliferation and the identity of the surface lining cell in rabbit aortas subjected to extensive deendothelialization. endothelial cells were selectively removed by passage of an inflated balloon catheter through the arterial lumen. the healing response was evaluated at intervals up to 36 weeks by several techniques: 1) permeability to evans blue, 2) reappearance of endothelial cells as indicated by the specific marker, adsorbe ...	1977	322503
solid-phase enzyme immunoassay for hepatitis b surface antigen.	a solid-phase enzyme immunoassay is described for measuring hepatitis b surface antigen in human serum or plasma. immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. in the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specifici ...	1977	322892
ultrastructural immunocytochemical localisation of lysozyme in human bronchial glands.	an immunocytochemical unlabelled antibody method using rabbit antihuman lysozyme, antirabbit immunoglobulin, and soluble rabbit antihorseradish peroxidase/horseradish peroxidase complexes was used to study the fine structural distribution of lysozyme in human bronchial glands. none was identified in mucous cells but there was heavy staining of the serous cell granules. the serous cell granules were not stained uniformly, suggesting the presence of other secretory products but lysozyme secretion ...	1977	325682
the localization of proteoglycans and glycoproteins in the hyaline cartilage.	antibodies to proteoglycan (pg) and glycoprotein of bovine nasal cartilage were conjugated with fluorescein isothiocyanate and with horseradish peroxidase. hyaluronidase digestion of cartilage tissue-specimens increased the intensity of immune reactions; pronase digestion or extraction with 4 m guanidinium chloride abolished the staining. in the intercellular matrix fine filaments beaded with small granules were seen forming an irregular network. the interstices of the network are filled with co ...	1977	332674
immune complex receptors on cell surfaces. iii. topography of macrophage receptors demonstrated by new scanning electron microscopic peroxidase marker.	receptors for immune complexes have been localized on rabbit alveolar macrophages with scanning electron microscopy by exposing the cells first to a soluble immune complex composed of horseradish peroxidase and antibody to horseradish peroxidase, and then incubating with a benzidine-containing substrate that yields crystalline reaction product. receptors were visualized by this means as sites of attachment of laminated slender crystals that were easily distinguished from macrophage surface struc ...	1977	333019
evaluation of some of the parameters of the enzyme-linked immunospecific assay.	in an attempt to standardize the procedure for the enzyme-linked immunospecific assay, several parameters were examined. it was determined that horseradish peroxidase was the enzyme of choice and that several brands of polystyrene tubes and plates could be used. the test was considerably shortened by omitting long incubation steps previously used for eliminating background fixation of conjugate. the reproducibility of the procedure proved to be excellent, but reactivity curves indicated that use ...	1977	333043