Publications

gastric acid secretion in patients with typhoid fever.	gastric secretory studies were carried out in the following groups of patients: group a, 30 salmonella typhi positive enteric patients, group b, 15 non-enteric fever patients and group c, 20 healthy controls. patients with typhoid were divided into two subgroups on the basis of the severity of the disease: group a1 (17) consisted of uncomplicated cases of enteric fever and group a2 (13) comprised of those who had associated complications. during convalescence both groups of typhoid patients show ...	1985	3996939
cadmium chloride susceptibility, a characteristic of campylobacter spp.	we report a simple diagnostic characteristic useful in the presumptive identification of campylobacter jejuni and campylobacter coli. filter paper disks impregnated with cadmium chloride were placed on streaked agar medium. zones of growth inhibition for campylobacter spp. occurred at 1.25 micrograms per disk. other enteropathogens (salmonella spp., shigella spp., vibrio cholerae, vibrio parahaemolyticus, escherichia coli, and yersinia enterocolitica) were resistant to at least 40 micrograms per ...	1985	3998099
enhancement of encephalomyeloradiculitis in mice sensitized with spinal cord tissue and infected with lactate dehydrogenase-elevating virus.	c57br/cdj mice develop encephalomyeloradiculitis following peripheral inoculation of the c strain of lactate dehydrogenase-elevating virus (ldv-c). we investigated the effect of subcutaneous administration of syngeneic spinal cord homogenate mixed with phosphate buffered saline or emulsified in complete or incomplete freund's adjuvant 1 week before or after inoculation of ldv-c on the incidence and severity of central nervous system lesions. c57br/cdj mice developed an acute histological allergi ...	1985	3998124
the fetal tobacco syndrome.		1985	3999279
new observations in reducing body myopathy.	numerous autofluorescent reducing bodies were found in deltoid and biceps muscle biopsies of a 7-year-old girl with progressive, asymmetric muscle weakness. the structures were composed of tubular filaments as seen by electronmicroscopy. they seemed to have formed around nuclei. an excess of two unidentified proteins was found in muscle homogenates. this patient, like one previously reported, had high serum titers of antibodies to coxsackievirus, but her serum, when tested by indirect immunocyto ...	1985	4000482
[non-0:1 group vibrio cholerae isolated in clinical cases of acute diarrhea].		1985	4001752
vibrio cholerae infection and acquired immunity in an adult rabbit model.	we modified the rabbit model for enteric infection by vibrio cholerae developed by spira et al. and designated the ritard (for removable intestinal tie-adult rabbit diarrhea) model (20). our modification disc comprises a permanent ligation of the cecum (c) to prevent resorption of the fluid secreted by the small intestine, a temporary ligation of the small intestine (s) to enable the bacteria to colonize, and duodenal inoculation (di) of the challenge material. the main difference between ritard ...	1985	4002931
an investigation of the involvement of platelet activating factor in the non-histamine immediate hypersensitivity reaction in the rat peritoneal cavity.	synthetic paf-acether injected intraperitoneally (i.p.) into sham-sensitized rats, caused dose-dependent extravasation of plasma proteins into the peritoneal fluids. platelet aggregating activity was detected in the peritoneal fluids. in rats that had been passively sensitized for a non-histamine mediated immediate hypersensitivity reaction, antigen challenge caused extravasation of plasma proteins into the peritoneal fluids. no platelet aggregating activity was detected in these fluids. paf is ...	1985	4003197
retinal ganglion cell loss produced by intraocular kainic acid in cats: variation with somal size and eccentricity.	eight eyes of adult cats were injected with different doses of kainic acid (ka) and examined following survival times of either 5 or 12 days. at a survival time of 12 days, a dose of 76 nmol produced an 18% loss of ganglion cells in the center of the area centralis (ac), 70% loss at a location 2 mm from the ac, and 95% loss at a location 6 mm from the ac. larger doses (240, 760 and 2400 nmol) produced losses comparable to that observed for 76 nmol. for example, 2400 nmol produced a 35% loss in t ...	1985	4005551
[effects of anoxia and reoxygenation on the myocardial metabolism of hyperenergetic compounds in rats].		1985	4006712
the morphology of l-fucose, d-mannose specific lectin (sfl 100-2) produced by streptomyces no. 100-2.	the morphology of an l-fucose specific lectin, sel 100-2, from a streptomyces sp. was studied. electron microscopic observation showed that purified sfl 100-2 preparation consisted of particles homogeneous in size. the diameter was 25 nm. the digitized images of these particles had 2-fold rotation symmetry. the sedimentation coefficient (s020,w) was determined to be 20.6s. the particle weight and the stokes radius were calculated to be 8.0 x 10(5) daltons and 94 a, respectively, by three indepen ...	1985	4008461
the morphology of l-fucose, d-mannose specific lectin (sfl 100-2) produced by streptomyces no. 100-2.	the morphology of an l-fucose specific lectin, sel 100-2, from a streptomyces sp. was studied. electron microscopic observation showed that purified sfl 100-2 preparation consisted of particles homogeneous in size. the diameter was 25 nm. the digitized images of these particles had 2-fold rotation symmetry. the sedimentation coefficient (s020,w) was determined to be 20.6s. the particle weight and the stokes radius were calculated to be 8.0 x 10(5) daltons and 94 a, respectively, by three indepen ...	1985	4008461
in vitro and in vivo cholera toxin production by classical and el tor isolates of vibrio cholerae.	a comparative study was carried out on the in vitro production of cholera toxin by 19 vibrio cholerae el tor isolates from patients with cholera in south africa, one el tor isolate from a patient in malawi (a country approximately 1000 km north-northeast of south africa), 6 el tor and 12 classical type isolates from patients in bangladesh, and 5 culture collection classical strains. identical phage types and indistinguishable toxigenicities among the south african and malawi v. cholerae, represe ...	1985	4008618
effect of severe injury and critical illness on high-energy phosphates in human liver and muscle.	changes in hepatic and muscle high-energy phosphates in varying degrees of resting hypermetabolism were studied. fourteen severely injured and five critically ill patients with normal blood pressure were investigated: the results were compared with 14 normal controls. atp and adp levels in the muscle were significantly changed in acutely severe injury: lactate and pyruvate levels in the liver and muscle increased; glycogen level in the liver decreased. meanwhile, high-energy phosphates in both l ...	1985	4009768
relationship of renal prostaglandins to distal transport of sodium chloride in normokalemic and hypokalemic man.	the relationship between renal prostaglandins (pg) and electrolyte transport in the thick ascending limb of henle's loop (talh) was examined in 8 normal subjects and in 7 patients with hypokalemia, the latter including 4 cases of bartter's syndrome. distal fractional delivery and distal reabsorption of chloride and sodium were determined using clearance techniques under maximal volume expansion, and urinary excretion rates of pge2 and pgf2 alpha were measured. in the normal subjects, suppression ...	1985	4010649
pseudomonas nasal septal abscess.		1985	4012839
peritoneal neutrophilic cell response in necrotizing enterocolitis.	neutropenia commonly occurs in neonates with necrotizing enterocolitis (nec). in an attempt to determine the etiology of this neutropenia, we observed the peripheral and peritoneal neutrophil cell responses of seven infants at the time of surgery. six of seven patients had diminished peripheral neutrophil counts within 24 hours prior to surgery, with substantial mobilization of mature neutrophils into the peritoneum. we also looked at the rat peritoneum as a model for neutrophilic cell consumpti ...	1985	4014093
energy dependence of lung phosphatidylcholine biosynthesis studied with co hypoxia.	the energy dependence of lung phosphatidylcholine (pc) biosynthesis was evaluated by measuring incorporation of radiolabeled precursor substrates by the isolated perfused lung during carbon monoxide (co)-induced hypoxia. ventilation with gases containing 75 or 90% co (plus 5% o2 and 5% co2) resulted in a mean decrease of lung atp content by 29 and 53%, respectively, and a significant decrease of [u-14c]glucose incorporation into free fatty acids and the fatty acyl moiety of lung pc. [9,10-3h]pal ...	1985	4014459
transient q waves followed by left anterior fascicular block during exercise.	a 45 year old white man developed transient abnormal q waves and st segment elevation preceding left anterior fascicular block during exercise stress testing. the simultaneous disappearance of q waves and fascicular block suggested that the abnormal q waves were determined by an early septal conduction defect.	1985	4015909
spermine as a gastric antisecretory agent.		1985	4017838
a profile of the intestinal mucosal corticosteroid receptors in the domestic duck.	the corticosteroid receptor profile of the intestinal tract of the domestic duck (maintained on either a low-sodium (ls) or a high-sodium (hs) diet) was investigated. using tritiated triamcinolone acetonide (ta), corticosterone, or aldosterone as ligands, cytoplasmic mineralocorticoid receptors (mr, type i) and glucocorticoid receptors (gr, type ii) were found in the mucosal cytosol of the jejunum and colon with the following binding parameters: ls jejunum gr-kd, 3.4 nm; nmax, 245 fmol/mg protei ...	1985	4018554
humoral immunity to dietary antigens in healthy adults. occurrence, isotype and igg subclass distribution of serum antibodies to protein antigens.	the occurrence of antibodies to five dietary protein antigens in the sera from 21 healthy adults was investigated by a modified farr assay. antibody to ovalbumin (oa) occurred most frequently (90%) whereas only 24% had antibodies to alpha-lactalbumin (ala). no correlation was noted between the titer of antibodies against bovine serum albumin (bsa) and oa in the single individual. the avidity constants (10(8)-10(9) l/mol) and cross-reactivities against other albumins of anti-bsa antibodies in two ...	1985	4018884
appearance of dark keratinocytes following intracutaneous injection of cholera toxin in mouse skin.	intracutaneous injection of cholera toxin (ct), exotoxin of vibrio cholerae, induces epidermal hyperplasia in mice, rats, and hamsters. in the work reported here we found that, like other hyperplasiogenic compounds such as 12-o-tetradecanoylphorbol-13-acetate which are tumor promoters, ct induces dark basal keratinocytes (dark cells) in the epidermis of mice. these are distinct from other epidermal cells since they contain dense cytoplasm rich in ribosomes and tonofilaments. this was demonstrate ...	1985	4020159
surgical treatment for infective endocarditis--decision tree for the treatment and introduction of translocation method.	persistent fever during active-phase ie is an indication for surgical treatment in the light of the causative organism and the underlying disease. to counter worsening hemodynamics, surgery may be the only feasible way. surgery is performed when the patient does not respond to vasodilators and he shows an fs of less than 25%. the introduction of translocation provides a solution for the treatment of active ie. lesions, vegetations more than 5 mm in diameter, periannular abscess, and mycotic aneu ...	1985	4021068
[characteristics of monosynaptic connections between neurons of the callosal system and specific thalamic nuclei].	in the sensorimotor cortex of awake rabbits monosynaptic afferent and efferent connections of callosal neurons (cn) with ipsilateral specific thalamic nuclei (ventral posterolateral, ventral posteromedial, ventral lateral and anteroventral nuclei) were analyzed by electrophysiological method and compared with those of target-units of callosal fibres (tu). it was demonstrated that cn and tu differed in their monosynaptic inputs from the thalamus and in their axons projecting to these structures a ...	1985	4022178
fatty acid compositions of triacylglycerols and phospholipids in hen liver lipid before and after sexual maturity.	changes in fatty acid composition of triacylglycerols and phospholipids of the hen liver lipid on coming into egg production were described. of the major fatty acids of triacylglycerols, a high content of oleate and low content of stearate and polyenoic acids were observed in the hen liver at all ages (97 to 167 days of age). in contrast, high stearate and polyenoic acids were present in the liver phospholipids at all ages. the oleate content in triacylglycerols was twice that of phospholipids. ...	1985	4022907
the instability of intestinal clostridial colonisation in living beings.		1985	4022979
nag vibrio cholerae isolated from imported shellfish in guam.	a survey of imported shellfish available in public markets on the island of guam revealed the presence of nag vibrio cholerae contamination (10 of 38 sample lots positive) and high coliform counts (21 of 33 sample lots in excess of 2400 per 100 grams). nag v. cholerae contamination was associated with high coliform counts and origin in fresh or brackish waters rather than saltwater. further importation of fresh shellfish from other than approved sources was prohibited.	1985	4023801
[morphofunctional changes in microvessels of the stomach and pancreas in early periods following selective vagotomy].		1985	4024587
gastrin release after endoluminal perfusion with cisapride of the isolated antrum in the pig.		1985	4027024
[effect of phosphemide in combination with dexamethasone on dna structure in sarcoma 45].	it is shown that under the influence of phosphemide in combination with dexamethasone some characteristics of tumour dna (content of 5 methyl cytosine, temperature, interval and enthalpy of melting) approximate the corresponding characteristics of dna of healthy animals' liver better than under the influence of phosphemide only.	1985	4027269
increased maternal-fetal transport of fat in diabetes assessed by polyunsaturated fatty acid content in fetal lipids.	the distribution of fatty acids was determined by gas-liquid chromatography in total lipid and triglyceride fraction of extracts of several tissues of streptozotocin-diabetic rats and their fetuses on day 20 of pregnancy. in maternal rats, diabetes did not significantly affect fatty acid distribution apart from small changes in the relative content of linoleate in adipose tissue and liver. in the placenta, the fetal carcass and the fetal liver the triglyceride content increased approximately 2-f ...	1985	4027297
freeze-fracture replicas of rat ameloblasts reveal unusual membrane domains around gap junctions.	specimens of aldehyde-fixed and glycerol-impregnated tooth germs obtained from 1-2 day old rats were prepared for ultrathin section studies and for freeze-fracture, with the purpose of studying the structural organization of membranes of developing ameloblasts. in this report we describe unusual membrane domains which were found surrounding several ameloblast gap junctions. developing ameloblasts - when examined in ultrathin sections - exhibit gap junctions which appear straight, curved or invag ...	1985	4028186
subset specificity of lupus antilymphocyte antibodies studied by two-colour microfluorimetry.	subset specific lymphocytotoxic activity of lupus sera was studied by a combination of selective immunofluorescence labelling and complement-mediated lysis. most frequently death of b cells was detected. many of the sera caused lysis of t lymphocytes; selective cytotoxicity against suppressor t cells could be observed less frequently. all the anti-t4, anti-t8 and anti-b lymphocyte antibodies proved to be cold reactive.	1985	4030025
effects of histamine on bronchial artery blood flow and bronchomotor tone.	the effects of aerosolized 5% histamine (10 breaths) on bronchial artery blood flow (qbr), airflow resistance (rl), and pulmonary and systemic hemodynamics were studied in mechanically ventilated sheep anesthetized with pentobarbital sodium. histamine increased mean qbr and rl to 252 +/- 45 and 337 +/- 53% of base line, respectively. this effect was significantly different from base line for 30 min after challenge. the histamine-induced increase in rl was blocked by pretreatment with the histami ...	1985	4030570
effect of changes in the osmolarity of the growth medium on vibrio cholerae cells.	the rate and extent of lysis of vibrio cholerae cells under nongrowing conditions were dependent on the osmolarity of the growth medium. gross alterations in cellular morphology were observed when v. cholerae cells were grown in media of high and low osmolarity. the rate of lysis of v. cholerae cells under nongrowing conditions increased after treatment with chloramphenicol. chloramphenicol-treated v. cholerae 569b cells showed formation of sphaeroplast-like bodies in medium of high osmolarity, ...	1985	4030693
anomeric and other substrate specificity studies with myo-inositol-1-p synthase.	l-myo-inositol-1-phosphate synthase has been found to have at least a 5-fold preference for the beta-anomer of its natural substrate d-glc-6-p. the alpha-anomer appears to be an inhibitor of the reaction and may be converted to product as well. as well as showing an enzymatic preference for the equatorial c-1 hydroxyl of d-glc-6-p, our results suggest that it is the pyranose form of d-glc-6-p that binds to the enzyme and that ring-opening is an enzymatic step. we have also found d-2-dglc-6-p, d- ...	1985	4030785
irradiation and bcnu effects in the stroma in a rat transplanted glioma model: analysis of cerebral tumor bed effect.	f-344 rats were treated with whole-brain fractionated or unfractionated 137cs at several time intervals up to 6 weeks before they received intracerebral grafts of an ethylnitrosourea-induced rat astrocytoma (36b-10). rats treated with a single dose of 18 gy at 3- and 2 week intervals prior to tumor implantation died earlier than unirradiated control tumor recipients (p less than 0.05, log rank sum test). delivered 2 weeks prior to tumor grafting single doses of 10, 15, and 20, but not 5 gy also ...	1985	4033874
suppression of trypanosoma congolense, t. vivax and t. brucei infection rates in tsetse flies maintained on goats immunized with uncoated forms of trypanosomes grown in vitro.	significant suppression in the incidence of cyclical development of trypanosoma congolense, t. vivax and t. brucei occurred in glossina morsitans centralis maintained on goats immunized with in vitro-propagated uncoated forms of t. congolense, t. vivax and t. brucei, respectively. this was observed when tsetse given a t. congolense-infected feed were subsequently maintained on uninfected immunized goats and also when uninfected tsetse were fed on immunized goats infected with t. congolense, t. v ...	1985	4034247
the early revascularization of membranous bone.	the experimental finding that membranous onlay bone grafts maintain volume and viability to a greater extent than do endochondral grafts may be related to the more rapid vascularization of membranous bone. microangiographic techniques were used to study the rates of vascularization of membranous and endochondral bone grafts in adult white new zealand rabbits at 1, 3, 7, 14, and 21 days after bone grafting. vascularization patterns were quantified microscopically using a modified point-counting t ...	1985	4034769
isolation of porcine, canine and feline igg by affinity chromatography using protein a.	isolation of porcine, canine and feline igg has hitherto been achieved usually by deae-ion exchange chromatography and gel filtration. these procedures, however, are rather time-consuming, as they involve purification of igg. isolation of igg by affinity chromatography on a column of protein a-sepharose was attempted. igg of all three animal species was very easily isolated with a high yield. in the absence of any other method that allows isolation of igg of three animal species from a single co ...	1985	4035977
identification of thiamine diphosphate-binding proteins from rat liver supernatant.	transketolase (ec 2.2.1.1) was shown to be the sole enzyme protein bound to thiamine diphosphate (thdp) as coenzyme in the soluble fraction of rat liver. no new thdp-binding proteins with the molar ratio of thdp to protein of 1.0 were detected in the rat liver supernatant under the purification and assay conditions employed.	1985	4036754
involvement of the histidine residues in the ph-induced conformational change of histone h5.	comparative studies on the conformational stability of histones h1 and h5 have been carried out by monitoring the ph-induced conformational transitions of the proteins by cd and 1h nmr spectroscopies. the transition point of h1 agrees with the pka of the carboxyl groups of the acidic residues. in contrast, the transition of h5 is associated with the ionization of the histidine residues which exist exclusively in h5, as well as the deionization of the acidic residues. these observations, combined ...	1985	4037808
suitability of the p388f mouse lymphoma system for detecting potential carcinogens and mutagens.	the p388f mouse lymphoma (tk +/-) system has been evaluated further for use as a primary mammalian-cell test and as a complementary assay to the ames salmonella reverse-mutation assay. the system showed a clear dose response with three alkylating agents of previously demonstrated genotoxic potential and gave the anticipated positive result with benzo[a]pyrene in the presence of s-9 mix. in addition, while giving the expected negative result with sugar, the assay detected genotoxic activity in he ...	1985	4038675
inhibition of lipases by proteins. a kinetic study with dicaprin monolayers.	we report further investigations on protein inhibition of pancreatic and microbial lipases carried out with the monolayer technique. when beta-lactoglobulin a, melittin, serum albumin, myoglobin, and a protein inhibiting lipase from soybean were preincubated with a dicaprin film at a surface pressure of 35 dynes/cm, no activity was detected with horse pancreatic or rhizopus delemar lipases. by contrast, rhizopus arrhizus and geotrichum candidum lipase activities were not impaired under the same ...	1985	4038705
tetraphenylphosphonium ion is a true indicator of negative plasma-membrane potential in the yeast rhodotorula glutinis. experiments under osmotic stress and at low external ph values.	in the yeast rhodotorula glutinis, accumulation of the tetraphenylphosphonium ion (tpp+) was increased under conditions of osmotic stress, indicating a hyperpolarization of the negative membrane potential (delta psi). the following observations were consistent with the occurrence of hyperpolarized delta psi: enhanced accumulation of glucosamine, the uptake of which is also driven by delta psi; increased respiratory rate. the accumulation of tpp+ was gradually decreased by lowering the ph of cell ...	1985	4038875
grand rounds. immune globulin in the treatment of autoimmune thrombocytopenic purpura.	two patients with autoimmune thrombocytopenic purpura (atp) who received high-dose intravenous immune globulin before undergoing splenectomy are described, and the pathogenesis, clinical presentation, diagnosis, and treatment of chronic atp are reviewed. one patient was a 62-year-old white man who was admitted to the hospital with a history of thrombocytopenia and probable steroid-induced diabetes mellitus. the second patient was a 24-year-old black woman who was admitted for recurrent bleeding ...	1985	4039241
permeability studies of the guinea pig placental labyrinth. ii. tracer permeation and freeze-fracture of fetal endothelium.	permeability of the fetal endothelium within the guinea pig placental labyrinth is studied by means of horseradish peroxidase (hrp) and ionic lanthanum as diffusion tracers. the paracellular transport of hrp is restricted by the occluding junctions of the fetal endothelium. in contrast, ionic lanthanum readily permeates most of the intercellular junctions and rapidly infiltrates the basal lamina. freeze-fracture replicas reveal zonulae occludentes connecting the fetal endothelial cells. the netw ...	1985	4040341
permeability studies of the guinea pig placental labyrinth. ii. tracer permeation and freeze-fracture of fetal endothelium.	permeability of the fetal endothelium within the guinea pig placental labyrinth is studied by means of horseradish peroxidase (hrp) and ionic lanthanum as diffusion tracers. the paracellular transport of hrp is restricted by the occluding junctions of the fetal endothelium. in contrast, ionic lanthanum readily permeates most of the intercellular junctions and rapidly infiltrates the basal lamina. freeze-fracture replicas reveal zonulae occludentes connecting the fetal endothelial cells. the netw ...	1985	4040341
local cerebral glucose utilization in normal female rats: variations during the estrous cycle and comparison with males.	the quantitative 2-[14c]deoxyglucose autoradiographic method was used to study the fluctuations of energy metabolism in discrete brain regions of female rats during the estrous cycle. a consistent though statistically nonsignificant cyclic variation in average glucose utilization of the brain as a whole was observed. highest levels of glucose utilization occurred during proestrus and metestrus, whereas lower rates were found during estrus and diestrus. statistically significant fluctuations were ...	1985	4040921
solubilization and reconstitution of the renal phosphate transporter.	proteins from brush-border membrane vesicles of rabbit kidney cortex were solubilized with 1% octylglucoside (protein to detergent ratio, 1:4 (w/w). the solubilized proteins (80.2 +/- 2.3% of the original brush-border proteins, n = 10, mean +/- s.e.) were reconstituted into artificial lipid vesicles or liposomes prepared from purified egg yolk phosphatidylcholine (80%) and cholesterol (20%). transport of pi into the proteoliposomes was measured by rapid filtration in the presence of a na+ or a k ...	1985	4041452
expression of cell surface glycoproteins in human melanoma cell lines with different tumorigenic properties.	human malignant melanoma cell lines characterized by either a high or a low ability to grow subcutaneously in athymic nude mice have been examined for their cell-surface glycoproteins. striking differences were demonstrated between these 2 groups. cells from lines of low tumorigenicity (lt group) displayed twice as much vibrio cholerae neuraminidase and galactose oxidase accessible glycoproteins as cells from lines of high tumorigenicity (ht group) and each group of cell lines could be character ...	1985	4044055
ultrastructural immunocytochemical co-localization of serotonin and pnmt in adrenal medullary vesicles.	previous immunocytochemical studies at the light microscopic level have demonstrated serotonin immunoreactivity in rat adrenal epinephrine-containing cells. in this study we have used electron microscopic immunocytochemical methods to study the subcellular distribution of serotonin and the enzyme responsible for epinephrine biosynthesis, phenylethanolamine-n-methyltransferase (pnmt). the distribution of the immunostaining was compared in adjacent serial thin sections using a post-embedding metho ...	1985	4044301
[early penicillin treatment can prevent cns symptoms in tick-transmitted borrelia meningitis].		1985	4046663
dermatophytes in sporting activities.		1985	4047096
pathogenicity of exophiala jeanselmei for ddy mice.	the pathogenicity of three cultures isolated as phialophora jeanselmei was compared with that of three cultures of phialophora gougerotii using ddy mice. one hundred and twenty mice were used. they were divided into 6 groups consisting of 20 each. each culture was evaluated in 20 mice. mice were inoculated intravenously with 0.2 ml of a 1% (wet weight/vol.) yeast-like cell suspension and sacrificed at adequate intervals until the 30th day. as results, 1) the virulence of the three cultures each ...	1985	4047124
effects of tobacco and distraction on sensory and slow cortical evoked potentials during task performance.	sensory and slow cortical evoked potentials (ep) of female smokers were investigated under two conditions in separate sham and real smoking sessions: a non-distraction (nd) condition consisting of a constant fore-period s1-s2, i.e., a single-choice reaction time situation with a tone (s1)/light (s2) key press sequence, and a task distraction (td) condition identical to nd with the addition of a short-term memory task consisting of four letters presented within the s1-s2 interval with the require ...	1985	4047375
increase in placental 15-hydroxy-prostaglandin dehydrogenase in the first half of human pregnancy.	nad-dependent 15-hydroxy-prostaglandin dehydrogenase (pgdh) activity was measured in homogenates of 25 human placentae obtained between 7 and 17 weeks of gestation. pgdh activity, expressed in nanomoles pgf2 alpha metabolized per min, ranged from 0.2 to 5.4 nmoles per mg placental protein and from 1.5 to 80 nmoles per g wet weight. pgdh activity per mg protein and per g weight increased significantly in function of gestational age (p less than 0.001). between 7-8 weeks' gestation and 15-16 weeks ...	1985	4048476
prognostic role of cell morphology of animal tumors.	the purpose of this presentation is to determine the prognostic role of cellular morphology in animal neoplasia. with some exceptions, cellular morphology is the single most accurate predictor of the prospective behavior of neoplasms. there is generally a positive correlation between the degree of malignancy and prognosis. the exceptions are a) morphologically malignant-appearing tumors following a benign course (e.g., canine histiocytoma, canine seminoma, equine sarcoid) and b) morphologically ...	1985	4048772
prognostic role of cell morphology of animal tumors.	the purpose of this presentation is to determine the prognostic role of cellular morphology in animal neoplasia. with some exceptions, cellular morphology is the single most accurate predictor of the prospective behavior of neoplasms. there is generally a positive correlation between the degree of malignancy and prognosis. the exceptions are a) morphologically malignant-appearing tumors following a benign course (e.g., canine histiocytoma, canine seminoma, equine sarcoid) and b) morphologically ...	1985	4048772
fluoridation of drinking water: effects on kidney stone formation.	the influence of fluoride in drinking water on stone formation was studied in animals and in "in vitro" crystallization experiments. in male wistar rats fluoride inhibits ethylenglycol induced calcification of the kidneys and stone formation. the in vitro results performed in synthetic urine exhibited a dose-dependent delay of crystal growth.	1985	4049603
fluoridation of drinking water: effects on kidney stone formation.	the influence of fluoride in drinking water on stone formation was studied in animals and in "in vitro" crystallization experiments. in male wistar rats fluoride inhibits ethylenglycol induced calcification of the kidneys and stone formation. the in vitro results performed in synthetic urine exhibited a dose-dependent delay of crystal growth.	1985	4049603
[tasks of social public health in medical research in the field of human reproduction].	social hygiene research on human reproduction extends both to the social (demographic) as well as to the individual aspects (successful family planning) of the reproductive process. the main points in both central aspects, which coordinate the gynecological aspects of reproductive behavior in this research, are outlined. one central concern of the policy aspects of social hygiene administration in the german democratic republic is the question of reproduction. the demographic effects of activ ...	1985	4049933
lymphocyte migration from peyer's patches by diapedesis through m cells into the intestinal lumen.		1985	4050593
cryptococcal meningitis: presentation as sudden deafness.	a patient presented with a bilateral profound hearing loss of sudden onset following a two-month neurologic illness. microscopy and culture of cerebrospinal fluid revealed cryptococcus neoformans. treatment with amphotericin b and 5-fluorocytosine failed to restore hearing. auditory brain stem response and electrical promontory stimulation suggest a profound deafness with poor neuronal survival. this is consistent with previous temporal bone histopathology reports in individuals dying of cryptoc ...	1985	4050983
[anesthesiologic problems in unsuspected extra-adrenal pheochromocytoma].	although surgical mortality associated with pheochromocytoma has been markedly reduced in recent years, due to improvements in medical and anesthesiologic management, surgery in patients with unsuspected pheochromocytoma is still accompanied by a high fatality rate. a 20-year-old man without history of hypertension and previously in good health is reported who presented for exploratory thoracotomy and removal of a highly vascular mediastinal tumor. intraoperatively a pheochromocytoma was suspect ...	1985	4051167
incidence of vibrio cholerae and related vibrios in a coastal lagoon and seawater influenced by lake discharges along an annual cycle.	most probable numbers of vibrio cholerae and related vibrios were determined in albufera lake, valencia, spain, and in coastal waters under the influence of the lake discharges over the course of an annual cycle. the influence of temperature, kind of water, and characteristics of the different sampling sites on the numbers of vibrios recovered was evaluated. maximum recovery of vibrios reached 10(3)/ml in both types of waters analyzed. v. cholerae numbers reached 10(3)/ml in the lake and 10(2) i ...	1985	4051487
vector diagrams in the evaluation of human gait.	from recordings of reaction forces on the feet in the vertical (z) and horizontal for-aft (x) direction during walking, x-z vector-diagrams (vdg) are constructed for the purpose of clinical use in the evaluation of gait. such use presupposes the existence of reference values. the parameters of vdg, ie, the maximal coordinates of the vectors, are dependent on weight, speed of walking, age, step length, and physical condition. a requirement of studies on reference values is that these variables as ...	1985	4051709
stimulus-related correlations between medial gastrocnemius muscle tension and homonymous motoneuron membrane potential result from non-linearities.	in anesthetized cats, medial gastrocnemius motor units (mus) were electrically stimulated via their ventral-root axons with independent random patterns. muscle tension and homonymous alpha-motoneuron (alpha-mn) membrane potential fluctuations in response to these stimuli were recorded simultaneously. cross-correlating these two signals in temporal relation to the stimuli showed the covariance of the two signals around their respective means. with moderate to strong mu-mn couplings indicated by c ...	1985	4052760
the roles of reactive gliosis and mitosis on tropic factor production in traumatized nervous system tissue.	proximal stumps of rat sciatic nerves were attached to inlet ends of y-shaped silastic implants and offered a 'choice' of growing toward an elvax pellet containing homogenate from previously crushed optic nerve which had been exposed to saline or cytosine arabinofuranoside (arac). previous studies indicate that arac administration inhibits reactive gliosis in crushed optic nerve. preferential or exclusive growth of axons occurred in implant forks attached to pellets containing saline- (vs drug-) ...	1985	4052768
targeting of scrapie lesions and spread of agent via the retino-tectal projection.	scrapie infectivity and degenerative vacuolation was initially localized within the contralateral superior colliculus following intraocular injection. the time course of these events was prolonged. with the me7 strain of scrapie in sincs7 genotype mice, infectivity began to rise in the superior colliculus from about 70 days, followed by the earliest asymmetrical lesions there from 120 days, with death occurring at about 250 days, at which time vacuolar degeneration was widespread in the brain. w ...	1985	4052769
pharmacokinetics of n'-nitrosonornicotine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in laboratory animals.	the pharmacokinetics of n'-nitrosonornicotine (nnn) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (nnk) in the syrian golden hamster, the cd-1 mouse, and the baboon were compared to the pharmacokinetics in the fischer rat. the formation and biological half-life of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (nnal), the major metabolite of nnk, was also studied in these animal species. the biological half-life of nnn in these 4 animal species ranged from 0.24 h to 3.06 h, that of nnk from ...	1985	4052989
structural parameters and natural occurrence of 2-deoxy-2,3-didehydro-n-glycoloylneuraminic acid.	2-deoxy-2,3-didehydro-n-glycoloylneuraminic acid has been found to occur in porcine, bovine and equine submandibular glands as well as in the urine of pig, horse and rat. this novel, unsaturated sialic acid was isolated by gel filtration and ion-exchange chromatography. final purification was achieved by column chromatography or by preparative thin-layer chromatography on cellulose. the structural analysis was performed by combined capillary gas-liquid chromatography/mass spectrometry. the vario ...	1985	4054116
canine liver isolation-perfusion at normo- and hyperthermic temperatures with perfluorochemical emulsion (fluosol-43).	a perfluorocarbon emulsion, fluosol-43, was used as a blood substitute for oxygen transport during isolation-perfusion of the dog liver at 37 and 43 degrees c. preservation of hepatic functional integrity was assessed through analysis of perfusate constituents and animal survival after perfusion. flow to the liver during perfusion was greater than 1 ml/min/g with one-third of total flow provided through the hepatic artery and two-thirds through the protal vein. perfusion duration was 3 h. the po ...	1985	4054188
characteristics of rat megakaryocyte colonies and their progenitors in agar culture.	the characteristics of megakaryocyte colonies that develop from megakaryocyte progenitors of rat bone marrow stimulated by rat spleen-conditioned medium (scm) in agar culture were investigated. colony frequency was optimal on day 7 and increased relative to both the number of cells plated and the concentration of scm used. plating efficiencies averaged 72 +/- 16 megakaryocyte colonies/10(5) cells with 0.1 ml scm/culture. colonies were categorized as small cell and big cell. small-cell colonies h ...	1985	4054241
[inhibitory neurons in the structural organization of cortical inhibition].		1985	4054361
[role of cortical inhibition in the analysis of auditory stimuli].		1985	4054363
comparison of api 20e, api rapid e, and api rapid nft for identification of members of the family vibrionaceae.	sixty isolates, from nine species of the family vibrionaceae, were tested by the api 20e, api rapid e, and api rapid nft systems. results were compared with those obtained with standard biochemicals. included were 7 aeromonas caviae isolates, 27 aeromonas hydrophilia isolates, 10 aeromonas sobria isolates, 3 plesiomonas shigelloides isolates, 3 vibrio alginolyticus isolates, 3 vibrio cholerae isolates, 1 vibrio fluvialis isolate, 5 vibrio parahaemolyticus isolates, and 1 vibrio vulnificus isolat ...	1985	4056003
the portacaval and splenocaval shunt in the normal rat. a morphometric and functional reevaluation.	consequences of a portacaval and a distal splenocaval shunt on the liver were examined in normal male rats with measurements of the fractional clearance of icg, of aminopyrine demethylation and of the hepatic ultrastructure with morphometric techniques. twenty days after a portacaval shunt liver weight/100 g body weight was 30% smaller than in sham-operated controls. the most pronounced reduction of 50% occurred with surface area of the rer, whereas hepatocyte volumes, ser-surface area, mitochon ...	1985	4056341
inherited nuclear cataracts in the morgan horse.	congenital cataracts affecting the fetal and embryonal lens nucleus were found in 12 morgan horses. ten of the 12 affected animals were sired by the same stallion and the condition also affected his female half sibling. although females were almost three times more likely to be affected than males (9 vs 3), the difference was not significant. the ratio of 11 normal to 10 affected offspring by the affected stallion is compatible with an autosomal dominant mode of inheritance.	1985	4056369
functional characteristics of the calf muscles of the rat.	length-force relations, both active and passive, and twitch contraction characteristics were quantified for the entire complex of the superficial calf muscles, as well as individually for the mm. soleus, plantaris, and gastrocnemius, caput mediale and laterale, of eight male wistar rats. the m. soleus composes approximately 5% of the weight and cross-sectional area of the entire group of superficial calf muscles and is the only muscle of the group containing mainly slow-twitch fibers. the other ...	1985	4057263
effect of neuraminidase treatment of cells and effect of soluble glycoproteins on type 3 reovirus attachment to murine l cells.	the effect of pretreatment of murine l cells with bacterial neuraminidases on type 3 reovirus attachment was examined. we observed that such treatments resulted in a 60 to 80% decrease of subsequent attachment of 35s-labeled type 3 reovirus in a time- and dose-dependent manner. this result was specific for removal of cell surface sialic acid residues since the specific neuraminidase inhibitor 2-deoxy-2,3-dehydro-n-acetyl neuraminic acid completely prevented the observed effect. although the tota ...	1985	4057353
[ultrastructural and morphometric aspects of fibroblasts in the endometrium of rats during the estrus cycle].		1985	4059606
isolation and characterization of influenza c virus inhibitors in rat serum.	two inhibitors against haemagglutination by influenza c virus were isolated from pooled sera of normal rats by sequential chromatography on blue sepharose cl 6b, ultrogel aca 2, and deae-cellulose. the two inhibitors were identified as alpha 1-macroglobulin and murinoglobulin by comparison with the authentic samples. these inhibitors abolished the haemagglutination by influenza c virus strains but did not affect the haemagglutination by influenza a and b virus strains. haemagglutination inhibiti ...	1985	4060850
[modifications in relation to age in the number and diameter of nerve fibers in dorsal spinal roots of the rat].		1985	4062475
[development of glands of internal secretion in birds and immaturely-born mammals].	certain general problems on foundation of the hormonal regulation mechanisms during the prenatal and early postnatal ontogenesis are discussed basing on the authors' own results and on the literature data. they concern development of the hypophysis and peripheral target-glands in immaturely-bone mammalia and fowls. the data on foundation of the endocrine glands functional activity are compared in rats and hens. the notion on a relative autonomous hormonal function of the peripheral endocrine gla ...	1985	4062579
proteolytic cleavage of [3h]nitrobenzylthioinosine-labelled nucleoside transporter in human erythrocytes.	the transmembrane topology of the nucleoside transporter of human erythrocytes, which had been covalently photolabelled with [3h]nitrobenzylthioinosine, was investigated by monitoring the effect of proteinases applied to intact erythrocytes and unsealed membrane preparations. treatment of unsealed membranes with low concentrations of trypsin and chymotrypsin at 1 degree c cleaved the nucleoside transporter, a band 4.5 polypeptide, apparent mr 66 000-45 000, to yield two radioactive fragments wit ...	1985	4062878
mobilization of extracellularly bound ca2+ during high k+ and norepinephrine stimulation of the rabbit aorta.	the effects of alpha-adrenergic stimulation and high k+-induced membrane depolarization on 45ca2+ uptake and tension generation in the rabbit aorta were investigated. tension and unidirectional 45ca2+ uptake were increased by both stimulants. moreover, this 'steady-state' uptake remained elevated for as long as the stimulants were present. when tissues were preincubated in 45ca2+-containing pss prior to the ne or high k+ challenge the resulting ca2+ uptake showed an 'initial burst' of uptake whi ...	1985	4063534
localization of [3h]nitrobenzylthioinosine binding sites in rat spinal cord and primary afferent neurons.	the distribution of [3h]nitrobenzylthioinosine ([3h]nbi) binding to nucleoside transport sites in rat spinal cord and spinal roots was examined using membrane binding and autoradiographic techniques. a single class of high affinity binding sites having dissociation constants (kd) between 0.42 +/- 0.05 and 0.088 +/- 0.012 nm was observed in dorsal and ventral spinal cord and their associated roots. the maximal number of binding sites (bmax) in dorsal and ventral spinal cord was 110.1 +/- 7.1 and ...	1985	4063811
a comparison of alpha 2-adrenoceptor regulation in brain and platelets.	functional responses and alpha 2-adrenoceptor radioligand binding were studied in brain and platelets of rabbits under a variety of circumstances. the effects of oestrogen treatment and maturation were studied in female rabbits and of aging and amitriptyline treatment in male rabbits. no correlation was found between changes in brain and platelets either in response or alpha 2-adrenoceptor ligand binding under any of the conditions examined.	1985	4063813
avulsions of the auricle.		1985	4064990
bacteriophage cp-t1 of vibrio cholerae. identification of the cell surface receptor.	the attachment site on the cell surface of vibrio cholerae for the bacteriophage cp-t1 has been determined. purified lipopolysaccharide from the inaba and ogawa serotypes, and of both the classical and el tor biotype of strains of v. cholerae show equal phage-inactivating capacities. lipopolysaccharide extracted from a cp-t1-resistant mutant has no phage-inactivating capacity. such mutants lack o-antigen as demonstrated by bactericidal assays utilizing a monoclonal antibody directed against o-an ...	1985	4065151
amino acid sequence of heat-stable enterotoxin produced by vibrio cholerae non-01.	the amino acid sequence of heat-stable enterotoxin, produced by vibrio cholerae non-01 and isolated from its culture supernatant, was determined by both edman degradation of native and reductively carboxymethylated enterotoxin and also a combination of fast atom bombardment mass spectrometry and carboxypeptidase y digestion of native enterotoxin to be as follows: ile-asp-cys-cys-glu-ile-cys-cys-asn-pro-ala-cys-phe-gly-cys-leu-asn. this sequence is very similar, but not identical, to those of hea ...	1985	4065341
the distribution of lactate dehydrogenase isoenzymes in adult human achilles tendons.	the authors have studied the ldh isoenzymes distribution in the human achilles tendons. connection between the age, sex, and enzyme activity were not found. in the achilles tendon were found strong activity of isoenzymes iv and v (m-type), and controversy them moderate activity of isoenzymes i and ii (h-type).	1985	4065385
role of cholera toxin in enteric colonization by vibrio cholerae o1 in rabbits.	the role of cholera toxin (ct) in mucosal colonization by vibrio cholerae o1 was studied in rabbits by using toxinogenic v. cholerae and nontoxinogenic (a-b+ or a-b-) recombinant mutants derived from them. after oral inoculation, toxinogenic strains colonized intestinal mucosa significantly more efficiently than did either a-b- or a-b+ mutants; average colonization was increased 1.5- to 30-fold with toxinogenic strains, depending on the inoculum used and the portion of intestine studied. additio ...	1985	4066032
brainstem connections of vagal afferent nerves in the ferret: an autoradiographic study.	using an autoradiographic technique the central connections of vagal afferent fibres in the ferret were studied. the results show that the principal site of termination is the ipsilateral nucleus of the tractus solitarius with smaller projections to the contralateral tractus nucleus, the dorsal motor nucleus of the vagus and the area postrema and subpostrema. the fibres cross the midline via the commissural nucleus of cajal. no evidence of vagal afferent fibres was found in the reticular formati ...	1985	4066482
role of suppressive interactions in the cochlear microphonic response to wide-band clicks.	cochlear microphonic responses (cm) were recorded from the guinea pig cochlea with conventional differential electrodes implanted in the first and second turns. the cm frequency functions were determined by calculating the fast fourier transform of responses to wide-band clicks. these frequency functions appeared to be dependent upon the spectrum of the click. this effect was interpreted as resulting from suppressive interactions between the various components of the click. it is suggested that ...	1985	4066521
[adaptive reactions of the thyroid system in patients with ischemic heart disease to graded physical exertion].	data reflecting thyroid response to rationed exercise (bicycle ergometry) in coronary patients are presented with respect to physical stress tolerance and clinical form of coronary disease. the results were compared with those obtained in healthy untrained male subjects. exercise was carried on until submaximum capacity was reached, or clinical and/or electrocardiographic signs of coronary insufficiency appeared. radioimmune assays of blood thyrotropin and total triiodothyronine (t3) and thyroxi ...	1985	4068456
changes in intravascular fluid composition following blood replacement with perfluorocarbon emulsion in the rat.	the effects of near total blood replacement with the proprietary perfluorocarbon emulsion, fluosol-da, have been examined in conscious, chronically catheterized rats. animals survived for up to 48 h with a haematocrit of less than 2%. no overt changes in cardiovascular or respiratory functions occurred during blood replacement but thereafter, animals showed a progressive deterioration of haemodynamic control. changes in intravascular fluid composition during and following exchange-transfusion ha ...	1985	4068605
[refractory anemia, thrombocytosis and deletion of the long arm of chromosome 5].		1985	4068829