Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| origins of the parasympathetic preganglionic fibers to the cat intestine as demonstrated by the horseradish peroxidase method. | 1978 | 667629 | |
| relative mobility of con a and anionic receptor sites on the cell surface of lymphocytes. a quantitative-cytochemical study. | events following the binding of concanavalin a and cationized ferritin to the surface of normal human peripheral blood lymphocytes were followed using ultracytochemical methods. in this paper, we described a technique involving microdensitometry, which was employed in order to make a comparative cytochemical quantitation of the dense horseradish peroxidase-diaminobenzidine reaction products representing the states of concanavalin a receptors under various conditions of distribution and redistrib ... | 1978 | 670683 |
| terminal arbors of axons that have formed abnormal connections. | ther terminal arbors of individual retinogeniculate axons that have been induced to grow into an inappropriate geniculate layer have been revealed for light and electron microscopic study by being filled with horseradish peroxidase. after a unilateral ocular enucleation in kittens, single axons from the surviving eye show terminal arbors not only within their own geniculate layers but also in the denervated layers. the new, abnormal arbors arise from the terminal segments of arbors that lie with ... | 1978 | 675248 |
| fine structure of tight junctions between rat choroidal cells after osmotic opening induced by urea and sucrose. | after ventriculo-cisternal perfusion of hypertonic urea or sucrose, both the choroid plexus permeability to horseradish peroxidase and the structure of tight junctions between choroidal cells are modified. intercellular spaces are swollen, continuous ridges are fragmented and intrajunctional spaces are invested by many membranous particles. these morphological alterations appear to be reversible. these ultrastructural data are related to an osmotic maladjustment induced by the introduction of hy ... | 1978 | 675669 |
| limbic projections to the cat thalamus. a horseradish peroxidase study. | retrograde axonal transport of horseradish peroxidase was used to identify the afferent projection systems of the anterior thalamic nuclei in the cat. the results indicate that fibres projecting to the three anterior nuclei of the thalamus arise from the hippocampal region, septum and cingulate cortex unilaterally and from the hypothalamus (mamillary nuclei) both bilaterally and unilaterally. the cells of the medial mamillary nuclei send their axons to the ipsilateral av and am nuclei whereas th ... | 1978 | 676656 |
| barriers of peripheral nerve towards exogenous peroxidase in normal and protein deprived rats. | the protective barriers of the sciatic nerve in protein deprived and normal rats have been studied. horseradish peroxidase (hrp) was applied extra-neurally in rats aged between 4 and 26 weeks. the spread of the tracer was investigated 2 h, 24 h, and 3 days after application. passage of tracer into the endoneurium was found in all rats but decreased with age. within the endoneurium, hrp was phagocytized by normally occurring endoneurial histiocytes, which constitute part of the protective barrier ... | 1978 | 676677 |
| increased vesicular transport of horseradish peroxidase across the blood-brain barrier after chemical induction of hypertension. | 1978 | 676899 | |
| hypertension-induced cerebral atherosclerosis in the cholesterol-fed rabbit. | foam cell lesions were found in cholesterol-fed rabbits with induced hypertension, particularly in intimal cushions at branching sites, where permeability to horseradish peroxidase was enhanced. permeability to horseradish peroxidase was enhanced at the edge of intimal cushions without foam cell accumulation. this finding suggests that permeability is increased before foam cell infiltration. no foam cell lesions were observed in the intima of cerebral arteries distant from branching sites, but i ... | 1978 | 678315 |
| afferent connections of the entorhinal area in the rat as demonstrated by retrograde cell-labeling with horseradish peroxidase. | the entorhinal cortex (ec) of the rat has been divided into medial (mea) and lateral (lea) subdivisions. in order to analyze its afferent connections, small deposits of horseradish peroxidase (hrp) were placed at various loci within ec. the patterns of retrograde cell-labeling charted in 18 such cases suggested that ec is projected upon by several allocortical and subcortical structures and that there are differences in the afferent connections of the two subdivisions. thus, although hrp injecti ... | 1978 | 679029 |
| anatomical evidence of direct projections from the nucleus of the solitary tract to the hypothalamus, amygdala, and other forebrain structures in the rat. | ascending projections from the caudal (general-visceroceptive) part of the nucleus of the solitary tract (nts) were studied experimentally in the rat by the aid of the anterograde autoradiographic and the retrograde horseradish peroxidase (hrp) tracer techniques. microelectrophoretic deposits of tritiated proline and leucine which involved the caudal part of the nts, the dorsal motor nucleus of the vagus (dmx), and portions of the hypoglossal nucleus, nucleus intercalatus and/or nucleus gracilis ... | 1978 | 679038 |
| localized changes in blood-brain barrier permeability following the administration of antineoplastic drugs. | the effects of four antineoplastic drugs on the permeability of the blood-brain barrier to evans blue-albumin and to horseradish peroxidase were studied in cats. extravasation of tracer in brain tissue was observed only rarely following the injection of methotrexate, cyclophosphamide, or vincristine. however, 5-fluorouracil (15 mg/kg) caused localized evans blue-albumin exudation in various gray and white matter areas in 8 of 13 cats to which the evans blue was administered 7 hr after the drug i ... | 1978 | 679202 |
| an enzyme-linked immunoassay for ferritin in human serum and rat plasma and the influence of the iron in serum ferritin on serum iron measurement, during acute hepatitis. | to measure human serum ferritin and rat plasma ferritin a non-competitive enzyme-linked immunoassay has been developed using horseradish peroxidase as the enzyme. in this assay it proved necessary to use heated rat plasma to obtain reproducible ferritin values. the heating procedure caused a loss of 38% of the plasma ferritin. rat plasma ferritin values have been corrected for this loss. the standard deviation, from duplicate normal human and rat samples is 10 ng ferritin/ml serum and 69 ng/ml p ... | 1978 | 679492 |
| the prefrontal cortex of the cat: anatomical subdivisions based on retrograde labeling of cells in the mediodorsal thalamic nucleus. | different areas of the frontal cortex of the cat were injected with small amounts of horseradish peroxidase. the region of labeled cells in the mediodorsal nucleus of the thalamus (md) were related to the injected areas. distinct relations between subdivisions of md and of the prefrontal cortex were established: a rather large central sector of md projects to the gyrus proreus and the anterior parts of the gyri sigmoideus, rectus, and frontalis. a narrow lateral band of anterior md neurons proje ... | 1978 | 680047 |
| pulmonary capillary and permeability during hemorrhagic shock. | in previous experiments from this laboratory horseradish peroxidase was used to study the structural and functional characteristics of the normal canine pulmonary capillary membrane. the present study used the same technique to try to determine if any change occurred in the pulmonary capillary as a result of hemorrhagic shock. we found that hemorrhagic shock caused a fall (5 expt) or no change (2 expt) in estimated pulmonary transcapillary pressure based on starling's equation. however, lymphati ... | 1978 | 681215 |
| increased endocytosis with lysosomal activation in skeletal muscle of dystrophic mouse. | endocytosis in dystrophic muscles was studied by a combination of biochemical, radiochemical, and light and electron microscopic techniques. it was observed that the uptake of horseradish peroxidase (hrp) and 3h-inulin in vitro was increased in leg skeletal muscles from dystrophic mice compared with littermate controls. endocytosis of hrp in vivo was also increased in dystrophic muscles. when hrp was administered intravenously, light microscopic examination of the muscles showed that the macromo ... | 1978 | 681982 |
| [horseradish peroxidase labeled sources of the spinobulbar and spinothalamic fiber systems of the cat brain]. | a dense accumulation of horseradish peroxidase (hrp) positive neurons was found after bulbar hrp injections in the ipsilateral dorsal grey matter and in the intermediate zone on both sides. after thalamic injections the labelled neurons were located bilaterally in the ventral part of the intermediate zone. a conclusion is made that cells of the origin of the spinobulbar and spinothalamic tracts are located in the dorsal horn and in the intermediate zone of the spinal cord. | 1978 | 683371 |
| interaction of phospholipid vesicles with rat hepatocytes in primary monolayer culture. | we studied the interaction of positively and negatively charged unilamellar and multilamellar phospholipid vesicles (liposomes) with rat-liver parenchymal cells in primary monolayer culture. radioactive liposomal phosphatidylcholine was taken up more rapidly and to a larger extent from unilamellar than from multilamellar vesicles. no significant difference in uptake characteristics was observed between vesicles of different charge. the presence of serum greatly reduced uptake of liposomal phosph ... | 1978 | 687666 |
| vascular permeability alterations to horseradish peroxidase in experimental brain injury. | protein uptake and transport within the brain stem vasculature of mechanically brain injured cats was studied by means of both light and electron microscopy utilizing intravenously injected horseradish peroxidase as the protein tracer. in animals sustaining low grade head injuries not of sufficient intensity to elicit either microscopic, intraparenchymal hemorrhages or subtle, neuropathological responses, peroxidase extravasation was noted both in the vascular walls and in the surrounding parenc ... | 1978 | 687980 |
| dopamine and noradrenaline neurons projecting to the septal area in the rat. | the organisation of the catecholamine innervation of the rat spetal area was investigated by means of the glyoxylic acid fluorescence method in combination with dopamine uptake studies, lesions and retrograde tracing of horseradish peroxidase. the following catecholamine systems to the septum could be established: 1. the locus coreuleus noradrenergic system. these axons are widespread in the septum forming a moderately dense innervation in the anterior hippocampus, the medial septal nucleus, the ... | 1978 | 688339 |
| amygdaloid projections to the mesencephalon, pons and medulla oblongata in the cat. | amygdalotegmental projections were studied in 26 cats after injections of horseradish peroxidase (hrp) in the diencephalon, midbrain and lower brain stem and in 6 cats after injection of 3h-leucine in the amygdala. following hrp injections in the posterior hypothalamus, periaqueductal gray (pag) and tegmentum many retrogradely labeled neurons were present in the central nucleus (ce) of the amygdala, primarily ipsilaterally. injections of hrp in the posterior hypothalamus and mesencephalon also r ... | 1978 | 689127 |
| the afferent connections of the main and the accessory olfactory bulb formations in the rat: an experimental hrp-study. | the afferent connections of the main and accessory olfactory bulbs in the rat were examined by injecting horseradish peroxidase (hrp) into one or the other of these structures either by microelectrophoresis or by hydraulic pressure. alternate sections were stained with newly developed hrp-procedures using either benzidine dihydrochloride (de olmos and heimer, '77) or tetramethyl-benzidine. eighteen to twenty-four hours after unilateral hrp injections confined to the main olfactory bulb, a large ... | 1978 | 690266 |
| nuclei of the solitary tract: efferent projections to the lower brain stem and spinal cord of the cat. | the efferent projections from the solitary complex to the lower brain stem and spinal cord were studied in the cat with the autoradiographic anterograde axonal transport and retrograde horseradish peroxidase (hrp) techniques. a revised cytoarchitectonic description of the caudal two-thirds of the complex is presented in which the complex was subdivided into six nuclei: lateral, ventrolateral, intermediate, medial, parvocellular, and commissural solitary tract nuclei. following injections of 3h a ... | 1978 | 690272 |
| distribution of motoneurones to the neck muscles, biventer cervicis, splenius and complexus in the cat. | the distribution of motoneurones to the cat dorsal neck muscles biventer cervicis, splenius and complexus has been examined using the technique of retrograde horseradish peroxidase transport. a comparison was initially made of hrp uptake into motoneurones produced either by injecting hrp solution directly into a single neck muscle or by exposing the cut ends of motor nerves to an hrp solution. the injection of hrp into single neck muscles was found to produce widespread labelling of motoneurones ... | 1978 | 690273 |
| spinal and medullary input to the lateral cervical nucleus. | the distributions of spinal and medullary cells projecting to the lateral cervical nucleus (lcn) have been investigated in young cats and dogs using the retrograde horseradish peroxidase (hrp) technique. labeled spinal cells, whose axons contribute to the spinocervical tract (sct), were found at all levels of the spinal cord ipsilateral to the injection sites. no significant differences were found between cat and dog, nor between cases with single injections at different levels of the lcn. sct c ... | 1978 | 690282 |
| the localization of the motor neurons innervating the extraocular muscles in the oculomotor nuclei of the cat and rabbit, using horseradish peroxidase. | the localization of the motor neurons innervating the extraocular muscles in the oculomotor nuclei of adult cats and rabbits was investigated by means of retrograde labelling with horseradish peroxidase (hrp). the groups consisting of the motor neurons innervating an individual muscle lay in the nucleus as elongated columns extending in a longitudinal direction. the position of each group in the transverse section varied according to the rostro-caudal level of the nucleus. in the cat and rabbit, ... | 1978 | 690283 |
| organization and projections of the neurons in the dorsal tegmental area of the rat. | the organization and projection of the neurons in the dorsal tegmental area at the locus coeruleus level of the rat have been investigated by means of the horseradish peroxidase (hrp) method, combined with monoamine oxidase staining to identify the noradrenaline (na) neurons. judging from the anatomical aspects elucidated in this study, this area is composed of various different cell groups. although the functional role of these neuronal groups remains to be determined, the present study strongl ... | 1978 | 690413 |
| cells of origin of subcortical afferents to the caudate nucleus: a horseradish peroxidase study in the cat. | the horseradish peroxidase tracing method was used to investigate the sources of subcortical input to the caudate nucleus in the cat. of considerable interest was the finding, not previously described, that labeled neurons were present outside of the intralaminar complex, in the ventral anterior and mediodorsal thalamic nuclei. another new finding was that retrogradely labeled cells were present within the basolateral amygdaloid nucleus, following the caudate injections. in addition, neurons con ... | 1978 | 698789 |
| trigeminal ascending projections in the rabbit, studied with horseradish peroxidase. | 1978 | 698827 | |
| the t-tubule system in the myocardia of the sand rat and mouse as demonstrated by horseradish peroxidase. | the transverse tubule (t-tubule) system in papillary muscles of the sand rat and the mouse were studied with the aid of a diffusion tracer (horseradish peroxidase). the t-tubule system in the sand rat showed a typical mammalian pattern with sarcolemmal tubules invaginating at the z-band level of the sarcomere. these tubules follow a transverse direction in the cell with frequent longitudinal side-branches which connect tubules at different z-band levels. in the mouse myocardium, the t-tubules al ... | 1978 | 699013 |
| studies on the ultrastructure and permeability of the hemotrichorial placenta. i. intercellular junctions of layer i and tracer administration into the maternal compartment. | the distribution of lanthanum chloride and horseradish peroxidase within the full-term chorioallantoic placenta of the rat was investigated 1 to 20 min after administration of these tracers into the maternal blood circulation. both tracers rapidly penetrate trophoblastic layer i and diffuse into the interspace between layers i and ii. they are localized in extensive infoldings and caveolae of the outer surface of layer ii. the syncytial character of layer ii is confirmed at this development stag ... | 1978 | 699024 |
| studies on the ultrastructure and permeability of the hemotrichorial placenta. ii. fetal capillaries and tracer administration into the fetal blood circulation. | the distribution of horseradish peroxidase and lanthanum chloride within the full term chorioallantoic placenta of the rat was examined after administration of these tracers into the umbilical artery. both tracers rapidly traverse the capillary endothelium. transendothelial channels, fenestrations and micropinocytotic vesicles provide the main pathways. intercellular clefts which are either patent or interrupted by leaky intercellular junctions, also contribute to a rapid passage of low and high ... | 1978 | 699025 |
| the t-tubule system in the specialized and general myocardium of the rat. | the t-tubule system in cardiac muscle cells has been investigated with the electron microscope in 10 adult rats after infiltration with horseradish peroxidase. all cardiac muscle cells possess a t-system, but its complexity varies according to the region of the heart. it is most extensive in the general ventricular myocardium where there are primary, secondary and tertiary transverse tubules as well as longitudinal elements, and there are numerous couplings between the t-system and the sarcoplas ... | 1978 | 701190 |
| retrieval and recycling of synaptic vesicle membrane in pinched-off nerve terminals (synaptosomes). | the morphological features of pinched-off presynaptic nerve terminals (synaptosomes) from rat brain were examined with electron microscope techniques; in many experiments, an extracellular marked (horseradish peroxidase or colloidal thorium dioxide) was included in the incubation media. when incubated in physiological saline, most terminals appeared approximately spherical, and were filled with small (approximately 400-a diameter) "synaptic vesicles"; mitochondria were also present in many of th ... | 1978 | 701355 |
| distribution of horseradish peroxidase (hrp)-anti-hrp immune complexes in mouse spleen with special reference to follicular dendritic cells. | the distribution of immune complexes has been studied in mouse spleen stimulated to contain many germinal centers (gc's). horseradish peroxidase (hrp)-anti-hrp complexes were used as an appropriately precise and sensitive model. we were primarily interested in the relative abilities of three cell types to interact with complexes: lymphocytes, macrophages, and follicular dendritic cells (fdc's). the latter are distinctive, nonendocytic, stellate cells located primarily at the transition of mantle ... | 1978 | 701370 |
| open junctions in the endothelium of the postcapillary venules of the diaphragm. | we have previously established that approximately 30% of the endothelial junctions in the pericytic venules of the mouse diaphragm are open to a gap of approximately 30--60 a, and are fully permeated by hemeundecapeptide (h11p) (mol diam approximately 20 a). to estimate the size limit for molecules that can permeate these junctions, we have administered graded tracers intravenously and studied their behavior at the level of pericytic venules in bipolar microvascular fields (bmfs) in the mouse di ... | 1978 | 701375 |
| cell bodies of origin of reticular projections from the superior colliculus in the cat: an experimental study with the use of horseradish peroxidase as a tracer. | by use of the retrograde axonal transport of horseradish peroxidase (hrp), the projection from the superior colliculus (sc) to the brain stem reticular formation (rf) was investigated in the cat. a 0.2-0.5 microliter of a 50% suspension of sigma vi hrp was injected stereotactically in various portions of the pontomedullary rf, and, as a control to the injection to the rf, in the inferior olive or in the spinal cord. labeled cells were found within and deep to the intermediate gray layer of the s ... | 1978 | 701486 |
| laminar origins of spinothalamic projections in the cat as determined by the retrograde transport of horseradish peroxidase. | the method of retrograde axonal transport of horseradish peroxidase (hrp) was used to identify the locations of cells of origin of the spinothalamic tract in the cat. injections of from 0.2-3.0 microliter of 30% hrp were made unilaterally in various regions of the somatosensory thalamus. massive injections of the caudal thalamus in several cats showed the spinothalamic cells of origin to be located mainly in laminae i, vii and viii in the lumbar enlargement, and in laminae i, v and vii-viii in t ... | 1978 | 701487 |
| anatomical and physiological properties of ipsilaterally projecting spinothalamic neurons in the second cervical segment of the cat's spinal cord. | anatomical and electrophysiological methods were used to investigate the projections and response properties of neurons in the second cervical (c2) spinal segment of the cat giving origin to a previously undescribed projection to the ipsilateral thalamus. the method of retrograde axonal transport of horseradish peroxidase (hrp) was used to identify neurons in c2 giving rise to thalamic projections. following large (3.0 microliter) thalamic hrp injections, a large number of labeled neurons was ob ... | 1978 | 701488 |
| differential projections of cat medullary raphe neurons demonstrated by retrograde labelling following spinal cord lesions. | neurons of the medullary raphe nuclei in cats were retrogradely labelled following injection of horseradish peroxidase (hrp) into the l6 spinal cord segment. brainstems were cut in sagittal section to facilitate examination of the rostral-caudal extent of raphe neurons projecting to the spinal cord. large numbers of hrp-labelled neurons were found in nucleus raphe magnus, nucleus raphe pallidus, and nucleus raphe obscurus (as well as a few neurons in nucleus raphe pontis). dorsal or ventral hemi ... | 1978 | 701490 |
| the olivocerebellar projection in the cat studied with the method of retrograde axonal transport of horseradish peroxidase. vii. the projection to lobulus simplex, crus i and ii. | the olivocerebellar projection to lobulus simplex, crus i and ii in the cat was investigated by means of retrograde axonal transport of horseradish peroxidase (hrp). the distribution of labeled cells in the inferior olive following hrp injections in lobulus simplex, crus i and ii confirmed the findings by brodal ('40b) that the rostral half of the principal olive projects to these areas of the cerebellar hemisphere. however, concerning details there are some differences in so far as the heaviest ... | 1978 | 701495 |
| a morphological study of the axons and recurrent axon collaterals of cat alpha-motoneurones supplying different hind-limb muscles. | 1. intracellular injections with horseradish peroxidasewere performed in cat alpha-motoneurones supplying various hind-limb muscles. 2. ten alpha-motoneurones from each of the quadriceps, posterior biceps, gastrocnemius-soleus and anterior tibial pools as well as from the pool supplying the short plantar muscles were collected for morphological analysis of the intramedullary axonal systems including the recurrent axon collaterals. 3. the diameter of the alpha-motor axons showed considerable vari ... | 1978 | 702381 |
| a morphological study of the axons and recurrent axon collaterals of cat alpha-motoneurones supplying different functional types of muscle unit. | 1. cat alpha-motoneurones supplying muscle units of the soleus s type and gastrocnemius s, fr and ff types were injected intracellularly with horseradish peroxidase. 2. ten alpha-motoneurones of each motor unit type were collected for morphological analysis of the intramedullary parts of their axonal systems including the recurrent axon collaterals. 3. the alpha-motor axon diameters in the spinal cord white matter were significantly smaller for the soleus motor units (mean = 5.2 micrometer) than ... | 1978 | 702385 |
| ultrastructural studies on blood-brain barrier dysfunction after cerebral air embolism in the rat. | male albino rats were anaesthetized with diazepam, injected with horseradish peroxidase and evans blue-labeled albumin and given an embolus of 0.01 ml air in the right common carotid artery after ligation of the external carotid branch. the pial arteries of the right cerebral hemisphere were stained blue, particularly the middle cerebral artery and its main arterial branchlets. ultrastructurally, some endothelial cells in the right middle cerebral artery, small arteries and arterioles showed a d ... | 1978 | 707046 |
| sites of cerebrovascular injury induced by radiographic contrast media. | the object of this study was to determine the type of cerebral vessel affected by injection of radiopaque contrast agents used in cerebral angiography. seventeen rabbits were prepared surgically for a left intracarotid injection of methylglucamine iothalamate (conray 60) or methylglucamine diatrizoate (reno-m-60). extravasations of the tracers, evans blue and horseradish peroxidase, occurred in the left half of the brain and occasionally in the right half. within those areas of blood-brain barri ... | 1978 | 707323 |
| developmental organization of raphe serotonin neuron groups in the rat. | the pre- and early postnatal development of serotonin neurons in the rat brainstem was studied using the fluorescence histochemical method. the technique utilized does not require drug pretreatment to visualize an intense serotonin fluorophore localized in neuronal perikarya, dendrites, and axons. all the serotonin neuron groups develop as bilateral nuclei which extend from the midbrain through the medulla. six of the nine groups undergo a midline fusion from embryonic day 18 (e 18) through post ... | 1978 | 707816 |
| protein thiolation and reversible protein-protein conjugation. n-succinimidyl 3-(2-pyridyldithio)propionate, a new heterobifunctional reagent. | a heterobifunctional reagent, n-succinimidyl 3-(2-pyridyldithio)propionate, was synthesized. its n-hydroxysuccinimide ester group reacts with amino groups and the 2-pyridyl disulphide structure reacts with aliphatic thiols. a new thiolation procedure for proteins is based on this reagent. the procedure involves two steps. first, 2-pyridyl disulphide structures are introduced into the protein by the reaction of some of its amino groups with the n-hydroxysuccinimide ester sie of the reagent. the p ... | 1978 | 708370 |
| a study of sensory projection from jaw muscles to the cerebral cortex in the rat. | the sensory projection from jaw muscles to the cerebral cortex have been studied in rats by electrophysiological and histochemical methods. electrical stimulation of individual masticatory muscles elicited bilateral responses in the cortical areas 8, 10, 2, and 2a. the following pathway was postulated to mediate these cortical responses; impulses of muscle origin are conducted in turn to the trigeminal mesencephalic tract nucleus (tmt), the contralateral thalamic nucleus ventralis posteromediali ... | 1978 | 713182 |
| subcortical projections to the prefrontal cortex in the rat as revealed by the horseradish peroxidase technique. | 1978 | 714251 | |
| long ascending projections from substantia gelatinosa rolandi and the subjacent dorsal horn in the rat. | small neurons of the substantia gelatinosa rolandi and the subjacent dorsal horn of the spinal cord have been thought to exert a direct modulatory effect only on neurons located within a distance of a few spinal segemnts. by using the technique of retorograde transport of horseradish peroxidase, however, it has been found that in the rat a significant number of these cells, particularly those of the subjacent dorsal horn, ascend many spinal segments to the lateral cervical nucleus and to the low ... | 1978 | 715454 |
| synapses of optic nerve afferents in the rat suprachiasmatic nucleus. i. identification, qualitative description, development and distribution. | synapses of optic nerve afferents (optic synapses) in the rat suprachiasmatic nucleus (scn) have been identified ultrastructurally. they are easily distinguished from other types of synapses. the optic boutons are characterized by the presence of large mitochondria with a swollen electron lucent matrix and an interconnected tubular system formed by their inner membrane. other, more variable features include: 1) a scattered pattern of synaptic vesicles which are found throughout the entire presyn ... | 1978 | 719729 |
| surface functions during mitosis i: phagocytosis, pinocytosis and mobility of surface-bound con a. | phagocytosis, pinocytosis and the surface distribution of concanavalin a (cona) have been analyzed during mitosis in several mammalian cell lines. use of the bisbenzimidazole dye, hoechst 33258, for chromosome staining after gentle fixation made possible the rapid identification and correlation of mitotic phase with surface properties. phagocytosis of both opsonized and nonopsonized particles is markedly depressed in mitotic cells of the mouse macrophage cell line j774.1. the uptake of opsonized ... | 1978 | 719746 |
| removal of horseradish peroxidase and fluorescein-labelled dextran from csf spaces of rabbit optic nerve. a light and electron microscope study. | 1978 | 720432 | |
| topographical organization of the striatonigral pathway revealed by anterograde and retrograde neuroanatomical tracing techniques. | l-[4,5-3h]leucine was injected stereotaxically into various regions of the rat neostriatum. light microscopic autoradiographic techniques were used to plot the entire efferent pathways of the neostriatum, and in particular, the projections to the substantia nigra (sn). the terminal distribution of the pathways projecting to the ipsilateral sn was predominantly restricted to the zona reticulata region. the dorsal part of the head of the striatum was found to innervate the anterior and medial port ... | 1978 | 721701 |
| early atherosclerotic lesions in rat aorta. effect of cholesterol feeding and immunization. | germ-free and conventional rats were fed cholesterol or normal pellets and part of both groups were immunized repeatedly with horseradish peroxidase (hrp). four days after the last injections all the rats were killed, aortas removed and prepared for immunocytology and chemical analysis. free peroxidase was not detected in the arterial wall and in all immunized rats only faint staining of free anti-hrp antibodies occurred in the intima. in contrast there was a consistent deposition of hrp-anti-hr ... | 1978 | 725328 |
| [capillary ultrastructure and permeability in the posterior lobe of the rat pituitary]. | the ultrastructure of capillaries and their permeability to horseradish peroxidase and ferritin in rat posterior pituitary has been studied. the capillary wall is formed mainly by flat endothelium penetrated by regularly situated fenestras. the majority of organelles is concentrated in the perikaryon. caveoles and pinocytotic vesicles are not numerous except for some perikaryons where they are concentrated along the basal surface. intracellular clefts are interrupted in the regions of fusion of ... | 1978 | 726059 |
| [comparative study of afferent connections in the habenula in rats and cats after injection of horseradish peroxidase]. | the habenular complex of 11 cats and 13 rats was injected with horse radish peroxydase (hrp) (30% solution, 0,05--0,4 microliter). retrograde labelling of cells was visualized after a survival period of 24 to 48 hours by means of the method of lavail et al. (1973). the resultats obtained with this method suggest significant species differences regarding the habenular afferents in cats and rats. one of the most striking differences is that the entopeduncular nucleus of the cat contains very few h ... | 1978 | 728629 |
| the effects of horseradish peroxidase on the concentration of 5-hydroxytryptamine and platelet counts in blood of rabbits and guinea pigs. | when hrp was injected i.v. in rabbits and guinea pigs, a rapid fall in the number of circulating platelets and the concentration of 5 ht occurred. platelet aggregates were trapped in lung capillaries. the aggregation was reversible and the return of platelets to the circulation concomitant with a rise in blood 5 ht concentration. | 1978 | 730554 |
| the superior colliculus control of pinna movements in the cat: possible anatomical connections. | possible anatomical pathways mediating superior colliculus control of pinna movements were determined in the cat using the orthograde autoradiographic tracing method and the retrograde horseradish peroxidase technique. this was done in the following manner. first, the division of the facial nucleus that innervates the pinna muscles was determined by injecting the pinna muscles with hrp and surveying the facial nucleus for retrogradely filled cells. second, the brainstem regions that project the ... | 1978 | 730846 |
| localization of sensory neurons traversing the stellate ganglion of the cat. | the distribution of sensory cells whose axons traverse the stellate ganglion and project via sympathetic cardiac nerves to the heart of the cat has been examined quantitatively. horseradish peroxidase (hrp) injected at multiple sites in the right stellate ganglion, or applied to the middle cardiac nerve, labelled small numbers of cells in the thoracic dorsal root ganglia (drg) from t1 to t8. these cells were most numerous between t2 and t5 and were consistently small (less than 40 micrometer) re ... | 1978 | 730853 |
| features of foreign proteins affecting their retrograde transport in axons of the visual system. | the retrograde axoplasmic transport of foreign proteins in the rat visual system shows certain specificities. the molecular features of these proteins which may underlie their entry into the retrograde phase have been examined using biochemical and morphologic techniques. of the isoenzymes of horseradish peroxidase (hrp), the basic isoenzyme c is strongly transported, while the acidic isoenzymes abeta and aalpha are transported weakly and not at all, respectively. decreasing the isoelectric poin ... | 1978 | 731278 |
| functional and anatomical consequences of neonatal visual cortical damage in superior colliculus of the golden hamster. | 1. in normal hamsters the visual cortex sends a retinotopically organized projection to the ipsilateral superior colliculus. 2. acute or chronic unilateral ablations of visual cortex in adult animals decrease the incidence of directionally selective cells encountered in the superficial laminae of the ipsilateral colliculus, but not in the deeper layers (those ventral to the stratum opticum). 3. unilateral ablations of visual cortex in infant hamsters induce an aberrant crossed projection to the ... | 1978 | 731286 |
| the distribution of motoneurones supplying chick hind limb muscles. | 1. the motor nuclei supplying many of the hind limb muscles were localized in late chick embryos (stage 36-37; 10-11 days) by utilizing the technique of retrograde transport of horseradish peroxidase. 2. each nucleus was found to be localized in a characteristic position in both the rostro-caudal and transverse plane of the spinal cord with only slight individual variation. 3. each motor nucleus consisted of an elongate, coherent cluster of labelled cells, with few cells occurring outside the cl ... | 1978 | 731549 |
| [pathways of substance penetration into the neurosecretory axons in the posterior lobe of the rat hypophysis]. | the penetration of horseradish peroxidase and ferritin into the neurosecretory axones in the rat's posterior pituitary has been studied with the electron microscope. markers were revealed in neurosecretory granules, in empty vesicles of the same size, in neurotubules, in lamellar bodies and in the axoplasm. markers were taken up from the extracellular space by micropinocytotic "synaptic" vesicles and probably by larger empty vesicles. it is supposed that the contents of these vesicles penetrate ... | 1978 | 734767 |
| the perineurial and blood-nerve barriers in experimental diabetes. | the permeabilities of the blood-nerve barrier and the perineurial barrier were investigated in alloxan and streptozotocin diabetic rats and in the mutant diabetic mouse [c57bl/ks(db/db)]. in the mouse model both fluorescence and electron microscopic techniques were employed using evans blue albumin (eba) and horseradish peroxidase (hrp), respectively, as exogeneous tracers. in the rat models only hrp was used. tracers were applied locally around the sciatic nerve in order to investigate the peri ... | 1978 | 735757 |
| fate of antigen after intravenous and intraarticular injection into rabbits. | to determine the manner in which small protein antigens enter the synovium and are localized, horseradish peroxidase (molecular weight 40,000) was injected by intravenous and intraarticular routes. passage of antigen from capillary lumen to joint space and vice versa occurred primarily across fenestrated vessels. antigen localized to collagen fibers and lining cells. at low concentrations of antigen, antigen was taken up predominantly by marcophages, whereas at higher concentrations both macroph ... | 1978 | 737015 |
| vascular permeability to proteins and peptides in the mouse pineal gland. | the permeability of fenestrated capillaries in the mouse pineal gland to proteins and peptides was demonstrated by means of ultrastructural tracers. horseradish peroxidase (hrp) and microperoxidase (mp) were injected intravenously and allowed to circulate for approximately 30 s, 1 min, 5 min, 1 or 2 h. the tissue was then fixed by vascular perfusion or by immersion with aldehydes. in all experiments a pronounced extravasation of hrp and mp occurred. transendothelial vesicular transport seemed to ... | 1978 | 737703 |
| lectin analysis of trypanosoma congolense bloodstream trypomastigote and culture procyclic surface saccharides by agglutination and electron microscopic technics. | living, intact bloodstream trypomastigotes and culture procyclic forms of trypanosoma congolense were tested for aggulination with the lectins concanavalin a (con a), phytohemagglutinin p (pp), wheat germ agglutinin (wga), soybean agglutinin (sba), and fucose binding protein (fbp). similar experiments were conducted with living bloodstream and culture forms treated with trypsin or dextranase. parasites were incubated for 30 min at 25 c in various concentrations of each lectin, then examined for ... | 1978 | 739411 |
| [auditory field projection into the ventrobasal complex of the thalamus studied by means of horseradish peroxidase and anterograde degeneration]. | 1978 | 743907 | |
| crossed disynaptic inhibition of sacral motoneurones. | 1. intracellular recording was made from motoneurones in lower sacral (s2 and s3) segments of the spinal cord in cats, to analyse the neuronal organization of the inhibition evoked in these motoneurones from contralateral afferents. 2. it was confirmed that stimulation of the lowest threshold afferents of contralateral dorsal roots evokes i.p.s.p.s with latencies similar to those of disynaptic i.p.s.p.s. evoked from group ia muscle spindle afferents in limb motoneurones. 3. the crossed disynapti ... | 1978 | 745104 |
| axonal transport of horseradish peroxidase: a new method for tracing nervous control of the bladder. | a new method for tracing the nervous control of the bladder is described. a protein substance, horseradish peroxidase, has been shown to be absorbed and transported by nerve fibers. the substance was injected into the bladder neck region of a series of dogs. after uptake by motor neurons the horseradish was carried to the spinal cord segment controlling micturition. implications regarding the neurophysiology of the urinary tract are discussed. | 1978 | 746608 |
| permeability of muscle capillaries to interstitially microinjected horseradish peroxidase. | 1978 | 748717 | |
| [projection of different ganglion cell classes in the albino rat retina. a study using horseradish peroxidase]. | in order to demonstrate the central projections of the different ganglion cell classes in the albino rat retina horseradish-peroxidase was injected into the superior colliculus (cs) and into the lateral geniculate nucleus (cgl). the study presents the following results: a) injection into the cs: labelled cells are only in the contralateral retina. topistically, the retino-collicular projection is in good agreement with the findings of physiological methods. the nasal half of the retina projects ... | 1978 | 749383 |
| changes of the stria vascularis induced by ethacrynic acid. | histological changes in the stria vascularis of guinea pigs after the injection of ethacrynic acid (50 mg/kg) were investigated using a transmission electron microscope and a scanning electron microscope. the changes in permeability in the stria vascularis was also observed using horseradish peroxidase as a tracer. the pathological changes originating in endothelial cells of stria blood vessels and intermediate cells spread into the marginal cells and basal cells. the most remarkable changes wer ... | 1978 | 753207 |
| the immune response of the genital tract of the female rabbit following systemic and local immunization. | female rabbits were immunized with a model antigen, horseradish peroxidase (hrp), either locally in the reproductive tract or systemically via the intramuscular route. a sensitive immunoassay was developed to detect antibodies to the hrp in both serum and reproductive tract flushings. systemic immunization resulted in circulating and reproductive tract antibodies of the igg class, but none of the iga class. local immunization produced iga and igg antibodies in the reproductive tract, and also ig ... | 1978 | 756472 |
| [the catecholaminergic connections of the visual system of the albino rat (author's transl)]. | by using the glyoxylic acid method of de la torre and surgeon (1976), occurrence, distribution and origin of catecholamines were investigated in the visual system of the albino rat. a dense network of green fluorescent terminals and preterminals could be detected in the dlgn. the spreading of terminals of catecholaminergic fibres had been observed in laminae i and ii of the visual cortex. investigation of lamina i with the golgi-kopsch method showed axons the thickness of which was below 1 micro ... | 1978 | 105553 |
| mediation of pinocytosis in cultured arterial smooth muscle and endothelial cells by platelet-derived growth factor. | pinocytosis was measured in monkey aortic smooth muscle cells (smc), bovine aortic endothelial cells, and swiss 3t3 cells in culture as cellular uptake of [u-(14)c]sucrose and horseradish peroxidase (hrp) from the tissue culture medium. monkey arterial smc and swiss 3t3 cells were maintained in a quiescent state of growth at low cells density in medium containing 5 percent monkey plasma-derived serum (pds). replacement of pds with 5 percent monkey whole blood serum (wbs) from the same donor, or ... | 1978 | 103882 |
| anatomical localization of corneal afferent cells in the trigeminal ganglion. | corneal afferent cells were identified in the trigeminal ganglion in the cat and monkey by the method of retrograde axonal transport of horseradish peroxidase. the corneal cells were found only in the ophthalmic portion of the ganglion and were scattered randomly within that division. these data are compatible with clinical and other experimental descriptions of the organization of the trigeminal ganglion and sensory root. | 1978 | 104187 |
| spontaneous and osmotically-stimulated activity in slices of rat hypothalamus. | single unit activity was recorded from 400-500 mu m thick slices of rat hypothalamus, using either nacl- or horseradish peroxidase-filled glass micropipettes. spontaneous activity was present in the following hypothalamic loci: anterior hypothalamic-preoptic area, nucleus circularis, nucleus of the diagonal band of broca, paraventricular accessory nucleus, paraventricular nucleus (all portions), periventricular regions of the anterior hypothalamus, and the suprachiasmatic nucleus. the supraoptic ... | 1978 | 122715 |
| the identification of relay neurons in the dorsal lateral geniculate nucleus of monkeys using horseradish peroxidase. | intraaxonal retrograde transport of the protein horseradish peroxidase (hrp) was used to identify relay neurons in the dorsal lateral geniculate nucleus (lgn) of owl (aotus trivirgatus) and rhesus (macaca mulatta) monkeys. in both species, from 94.1-98.6% of the neurons within columns extending through both parvocellular and magnocellular layers were labeled following injection of hrp into striate cortex. labeled neurons were also identified in the thin ventral-most s(0) layers. although most of ... | 1978 | 102664 |
| ascending tracts of the lateral columns of the rat spinal cord: a study using the silver impregnation and horseradish peroxidase techniques. | 1978 | 83245 | |
| tissular immunoenzymatic detection of hepatic alphafetoprotein in human hepatomas. | 5 human cases of hepatoma have been chosen with respect to their different seric alpha-fetoprotein (alpha-fp) level and histological characters. cells producing alpha-fp have been studied with specific horseradish-peroxidase-labelled immunoglobulins. ultrastructural examination shows that alpha-fp is present in the cytoplasm of some tumoral hepatocytes. alpha-fp is also present in the cytoplasm of some rare nontumoral hepatocytes of a nonsecreting hepatoma. ultrastructural differences are descri ... | 1978 | 86473 |
| [distribution pattern in the guinea pig cochlea of intravitally injected peroxidase]. | following perilymphatic perfusion and injection into the cisterna cerebello-medullaris, respectively, the distribution pattern of horseradish peroxidase in the cochlea of the guinea pig was studied light and electron microscopically. the findings prove effective tight junctions (zonulae occludentes) between the cells of the epithelial lining of the endolymphatic compartment. at the level of the reticular membrane the tight junctions are far more extended than elsewhere at the cochlear duct epith ... | 1978 | 86244 |
| some immunohistochemical experiments aiming at the electron-microscopic in situ identification of a dental plaque microorganism--streptococcus mutans. | the aim of the present investigation was to test a procedure useful for the electron-microscopic in situ identification of the presumptive cariogenic microorganism streptococcus mutans (serotype d) growing in the human dental plaque. for this purpose, different parameters of an indirect immunohistochemical method were tested in three sets of experiments. in experiment set i, all serological and histochemical procedures were performed en bloc on specimens fixed only with glutaraldehyde before emb ... | 1978 | 84515 |
| electron microscopic study of the orthograde axonal transport of horseradish peroxidase in the supraoptico-neurohypophysial tract of the rat. | horseradish peroxidase (hrp) has been used as a protein tracer in order to visualize the ultrastructural sites of the orthograde transport of protein macromolecules in the hypothalamo-neurohypophysial tract of the rat. after a local injection of hrp within the supraoptic nucleus, the reaction product was observed: (1) mainly in tubules of the smooth endoplasmic reticulum in the more proximal part of the axons, and (2) in granules and microvesicles of the axon terminals. observations on thick sec ... | 1978 | 83193 |
| [localization of motoneurones of retractor bulbi muscles by retrograde transport of exogenous peroxidase in cats]. | retrograde transport of exogene horseradish peroxidase has been used to localize the retractor bulbi muscle motoneurones. these motoneurones are located ventro-laterally in the tegmental reticular field; they are grouped in a rostro-caudal column above the superior olive. they correspond to the accessory nucleus of the vie nerve described by old anatomists. | 1978 | 96996 |
| osmotic opening of the blood-brain barrier. | the blood-brain barrier at cerebral blood vessels is due to a continuous lining of endothelial cells, which are connected by tight junctions that restrict intercellular diffusion. the endothelium excludes most water-soluble solutes and proteins but supports facilitated stereospecific transport of monosaccharides and large neutral and basic amino acids. the barrier in different species can be made permeable by infusing a hypertonic solution of urea or arabinose into the internal carotid artery. e ... | 1978 | 97064 |
| experimental injury of the optic nerve with optic disc swelling. | an ultrastructural study utilizing horseradish peroxidase was performed to determine the mechanism and consequences of leakage of vascular protein following injury of the optic nerve. unilateral optic nerve injuries were produced in four rhesus monkeys by making a cautery lesion on the retrobulbar portion of the optic nerve. optic disc changes were followed with stereo fundus photography and fluorescein angiography. three to 14 days after injury horseradish peroxidase was given intravenously and ... | 1978 | 98667 |
| [retrograde axonal transport of horseradish peroxidase along the striato-thalamic pathways of cats]. | 1978 | 98719 | |
| an ontogenic study of the acute inflammatory response in the fetal rhesus monkey. ii. the mediation of increased vascular permeability by histamine, compound 48/80 and bradykinin. | vascular permeability as a function of age was evaluated quantitatively and morphologically in skin of fetal rhesus monkeys given vasoactive mediators. the response was examined at 90, 120 or 150 days of gestation or at birth and compared to the response of yearling rhesus monkeys. skin sites were challenged with histamine, compound 48/80 or bradykinin. evans' blue and horseradish peroxidase were used as markers of vascular integrity. evans' blue was quantitated spectrophotometrically. vascular ... | 1978 | 98895 |
| a horseradish peroxidase-autoradiographic study of parietopulvinar connections in saimiri sciureus. | descending connections from parietal cortex to pulvinar in squirrel monkey were investigated with the autoradiographic method. somatosensory areas i (si) and ii (sii) were found to project to the oral (puo) and medial (pum) subdivisions of the pulvinar. projections from the posterior parietal region were recorded in circumscribed areas of pum and the lateral (pul) and inferior (pui) subdivisions of pulvinar. retrograde labeling with horseradish peroxidase (hrp) demonstrated that rostral parietal ... | 1978 | 99328 |
| a comparative study of the neurons of origin of the spinocerebellar afferents in the rat, cat and squirrel monkey based on the retrograde transport of horseradish peroxidase. | the cell bodies of the neurons of the spinocerebellar pathways were examined using large injections of horseradish peroxidose into the cerebellum. sections of each spinal segment were examined with both the dab and the de olmos o-dianisidine techniques. results common to all three species were found. in clarke's nucleus, the central cervical nucleus, and the spinal border cells there were many heavily labeled cells. clarke's nucleus was found to project primarily ipsilaterally; the spinal border ... | 1978 | 99460 |
| the neuroanatomical organization of pathways between the dorsal lateral geniculate nucleus and visual cortex in old world and new world primates. | pathways between the dorsal lateral geniculate nucleus (dlgn) and visual cortex in old world (macaca, papio, erythrocebus, cercopithecus) and new world (saimiri, cebus) primates were studied after injections of horseradish peroxidase and h3 or s35 amino acids into the dlgn or visual cortex. trans-synaptic autoradiography was also used to study these pathways after an injection of h3 proline-fucose into one eye. the subsequent autoradiographs of visual cortex showed that old world primates have s ... | 1978 | 100530 |
| organizational features of the cat and monkey cerebellar nucleocortical projection. | the organization of the cerebellar nucleocortical projection in the cat and the monkey has been studied using orthograde and retrograde neuroanatomical tracing techniques. injections of tritiated leucine in the cat cerebellar nuclei orthogradely labeled nucleocortical fibers throughout their course to the cerebellar cortex. their branch points in the corpus medullare, in the folial white matter, and in the granular layer were evident from the dense, continuous distribution of silver grains overl ... | 1978 | 100532 |
| the placental fcgamma receptors studied using immune complexes of peroxidase. | immune complexes of horseradish peroxidase (hrp) and rabbit igg antibodies to hrp were used to study the fcgamma receptors in normal human placenta. cryostat sections of placental tissue were incubated with the complexes, and the peroxidase activity was revealed histochemically. the bound complexes were localized to the apical surface of the trophoblasts and endothelial cells of the fetal stem vessels. binding also occurred within the wall of some fetal vessels, to stromal cells and occasionally ... | 1978 | 100870 |
| spinothalamic tract neurons in the substantia gelatinosa. | the substantia gelatinosa of the mammalian spinal cord is generally believed to be a closed system; that is its neurons are thought to project only to the substantia gelatinosa of the same or the contralateral side. experiments in monkeys, using injections of the marker enzyme horseradish peroxidase, show that at least some neurons of the substantia gelatinosa project to the thalamus and thus belong to the spinothalamic tract. such neurons include two cell types intrinsic to the gelatinosa, the ... | 1978 | 102034 |
| tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non-carcinogenic blue reaction product with superior sensitivity for visualizing neural afferents and efferents. | tetramethyl benzidine (tmb) is a presumptively non-carcinogenic chromogen which yields a blue reaction-product at sites of horseradish peroxidase activity. sixty-six distinct procedures were performed in rats and monkeys in order to determine the optimal incubation parameters for tmb. as a result, a procedure is recommended whose sensitivity greatly surpasses that of a previously described benzidine dihydrochloride method. indeed, the sensitivity of this new method in demonstrating retrograde tr ... | 1978 | 24068 |
| effect of beta-lapachone on hydrogen peroxide production in trypanosoma cruzi. | beta-lapachone, an antimicrobial agent, markedly increase the generation of h2o2 in intact trypanosoma cruzi epimastigotes (y strain). increase in h2o2 was determined by the horseradish peroxidase-h2o2 compound ii formation as well as by a cytochemical technique. | 1978 | 24995 |
| concanavalin a receptors on the cell membrane of trypanosoma cruzi. | epimastigotes and trypomastigotes of t. cruzi obtained from acellular culture as well as bloodstream trypomastigotes agglutinate with concanavalin a (con a). con a-binding sites were also localized on the cell membrane by using the con a-horseradish peroxidase-diaminobenzidine method. passage of epimastigotes and trypomastigotes from acellular culture through deae-cellulose column did not affect con a-binding sites as detected by agglutination and electron miscroscopy. | 1978 | 28652 |
| reaction of horseradish peroxidase with azide and some implications for the heme environmental structure. nmr and kinetic studies. | the azide complex of horseradish peroxidase was studied by high resolution 1h and 15n nmr spectroscopy and by the temperature-jump method. the heme peripheral methyl proton peaks and the ligand 15n resonance were resolved to show that binding of azide by horseradish peroxidase occurs only in acidic solution below ph 6.5. it was also found that the chemical exchange rate of azide with the ferric enzyme was much faster on the 1h and 15n nmr time scale. this was further substantiated by kinetics of ... | 1978 | 31922 |
| electron microscopical study of retrograde axonal transport of horseradish peroxidase in the supraoptico-hypophyseal tract in rat. | 1978 | 75882 |