Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| layer i of striate cortex of tupaia glis and galago senegalensis: projections from thalamus and claustrum revealed by retrograde transport of horseradish peroxidase. | we have examined the origin of the subcortical projections to the superficial layers of the striate cortex in tupaia glis and galago senegalensis by using the retrograde transport of hrp. crystals of hrp were laid directly on the moist pial surface of the cortex which had been gently pricked with a small glass pipette. the diffusion of hrp was limited to layers i and ii by restricting the length of time that the hrp was in contact with the surface. following the application of hrp to the striate ... | 1979 | 110851 |
| horseradish peroxidase. xxxvi. on the difference between peroxidase and metmyoglobin. | 1979 | 486194 | |
| the oxidation and decarboxylation of retinoic acid by horseradish peroxidase. | the decarboxylation of retinoic acid by horseradish peroxidase was investigated. a marked increase in the yield of products was obtained. however, the data indicated the reaction was a nonenzymatic, heme catalyzed peroxidation. previously reported requirements for phosphate, oxygen and ferrous ion were eliminated when hydrogen peroxide was provided. peroxide also eliminated the edta and cyanide induced inhibition of the phosphate dependent system. in the presence of hydrogen peroxide, horseradis ... | 1979 | 476130 |
| the effect of hyperglycemia on brain capillary permeability in the lizard, anolis carolinensis. a freeze-fracture analysis of blood-brain barrier pathology. | the anatomical basis of the blood-brain barrier in the american chameleon, anolis carolinensis, is the system of tight intercellular junctions that occurs between apposed endothelial cells of brain capillaries. under normal physiological conditions, capillaries in the brain cortex of these animals remain sealed by interendothelial zonulae occludentes and, consequently, escape of exogenous tracer proteins such as horseradish peroxidase (hrp) into the extracellular compartment of the central nervo ... | 1979 | 466426 |
| new ultrastructural evidence for a protein transport system in endothelial cells of gerbil brains. | pathways for transfer of horseradish peroxidase (hrp) across the cerebral microvasculature were studied in mongolian gerbils after inducing either unilateral carotid-artery ligation or intracarotid air embolism. electron microscopy on samples from both ipsilateral and contralateral brain hemispheres showed the reaction product (hrp) in the endothelial cytoplasm of capillaries and arteriols in all animals; in the basal lamina, hrp was seen only after long (3--4 h) ischemic periods. hrp was seen w ... | 1979 | 474070 |
| electron paramagnetic resonance analyses of horseradish peroxidase in situ and after purification. | 1979 | 37888 | |
| localization of acetylcholine receptors by means of horseradish peroxidase-alpha-bungarotoxin during formation and development of the neuromuscular junction in the chick embryo. | the localization of acetylcholine receptors (achr) in the surface of developing myogenic cells of the chick embryo anterior and posterior latissimus dorsi muscles in relation to the process of innervation has been studied at the ultrastructural level utilizing a horseradish peroxidase-alpha-bungarotoxin conjugate. localized concentrations of achr were found in small regions 0.1-0.4 micron in width on the surface of myogenic cells of 10- to 14-d-old muscles. surface specializations consisting of ... | 1979 | 479297 |
| [thalamocortical projections of the anterior ventral nucleus: study using retrograde axonal transport of horseradish peroxidase]. | 1978 | 371568 | |
| fixation variables in horseradish peroxidase neurohistochemistry. i. the effect of fixation time and perfusion procedures upon enzyme activity. | in a series of neurohistochemical experiments the effect of aldehyde fixation upon the detection of horseradish peroxidase (hrp) was examined. these experiments demonstrated that: a) increments in fixation of as little as 1 hr significantly decreased the number of labeled neurons; 12-hr fixation abolished hrp activity in many neuronal populations and significantly reduced the apparent size of the injection site. b) this negative fixation effect was greatest where the hrp concentration was low (e ... | 1978 | 413864 |
| the projections of the entopeduncular nucleus and globus pallidus in rat as demonstrated by autoradiography and horseradish peroxidase histochemistry. | 1978 | 411809 | |
| some claustro-cortical connections in the cat and baboon as studied by retrograde horseradish peroxidase transport. | 1. the mammalian claustrum (cl) is a convergent multisensory structure of unknown function, and disputed ontogenetic origin. its cortical projections, hitherto unknown, have been studied in cat and baboon by means of the horseradish peroxidase (hrp) technique. hrp was injected into the gyrus proreus (frontal eye field) of cats, and separately into the frontal eye fields, visual areas, and motor-premotor areas of the baboon cortex. 2. differential retrograde transport to the cl was demonstrated, ... | 1978 | 412882 |
| effect of daily ethanol ingestion on intestinal permeability to macromolecules. | the effects of regular ethyl alcohol ingestion on morphological and permeability characteristics of the small intestine were assessed in mature rats using the tracer protein, horseradish peroxidase. thirty adult rats were divided into two groups and provided a standard commercial diet in pellet form. each morning, after an overnight fast, every animal in the experimental group was administered by gavage an aliquot of 20% ethanol; animals in the control group were provided aliquots of 20% sucrose ... | 1978 | 563673 |
| ultrastructural studies on blood-brain barrier dysfunction around cerebral stab wounds, aggravated by acute ethanol intoxication. | ethanol-intoxicated and non-intoxicated albino rats subjected to cerebral stab wounds were injected with evans blue-labelled albumin (eba) and horseradish peroxidase (hrp) 24 hours after the injury. the tracers were injected 30 and 2 minutes before perfusion fixation with glutaraldehyde and then prepared for light microscopy and electron microscopy. there was prominent leakage of peroxidase through chains of pinocytotic vesicles and transendothelial channels from blood, through the endothelial c ... | 1978 | 566999 |
| neurones within the "chemosensitive area" on the ventral surface of the brainstem which project to the intermediolateral column. | with the method of retrograde transport of horseradish peroxidase it has been demonstrated that neurones within the "chemosensitive area" of the brainstem project to the thoracic intermediolateral column. the function of these neurones is discussed in regard to the regulation of blood pressure. | 1978 | 567798 |
| proton nuclear magnetic resonance spectra of compounds i and ii of horseradish peroxidase. | enzymatic reaction intermediates of horseradish peroxidase, compounds i and ii, were studied by high-resolution nuclear magnetic resonance spectroscopy at 220 mhz. the heme peripheral proton peaks were successfully obtained in the downfield region of 50 to 80 ppm from 4,4-dimethyl-4-silapentane-5-sulfonate for compound i and of 10 to 20 ppm for compound ii at ph 9.2. this indicates that no isoporphyrin appears in the catalytic cycle of the enzyme. temperature dependences of the spectra also were ... | 1978 | 568936 |
| thalamic projections to s-i in macaque monkey. | the organization of thalamic input to functionally characterized zones in primary somatosensory cerebral cortex (s-i) of macaque monkeys (macaca mulatta) was investigated using the method of labelling by retrograde transport of horseradish peroxidase (hrp). it was found that the cell columns positioned at the posterior margin of the band of cortex representing a given body region receive thalamic input from a posterior level of the ventroposterior thalamic nucleus (vp), and that cell columns at ... | 1978 | 418081 |
| rapid preparation of peroxidase: anti-peroxidase complexes for immunocytochemical use. | soluble immune complexes of horseradish peroxidase and antibody to peroxidase (pap) have been widely used in the 'unlabelled antibody' method for the immunocytochemical detection of cellular antigens. this paper describes a simple and rapid method for preparation of these complexes by column chromatography of a mixture of the enzyme and the igg fraction of antiperoxidase antiserum on sephacryl s-200. the material eluting in the void column consists of stable soluble pap complexes, with a molar p ... | 1978 | 418119 |
| a study on the postulated transoptic regulation of the intraocular pressure. | the possibility that fibers regulating the intraocular pressure originate in the hypothalamus and course in the optic nerve, has been investigated in albino rabbits with different techniques. by means of horseradish perixodase tracing-technique, oculohypothalamic but no hypothalamoocular connections were observed. the water-loading effect on the intraocular pressure was studied after unilateral optic nerve transection. the transection was performed in three experimental groups in the following w ... | 1978 | 580841 |
| immunohistologic localization of antigen and antibody in antigen-induced arthritis using horseradish peroxidase (hpo) labeling. | 1978 | 401418 | |
| comparison of light-microscopic methods for visualizing horseradish peroxidase granules in stained and unstained tissue. | this study was directed toward a comparison of the effectiveness of three different light-microscopical methods for identifying and studying the features of cells that contain horseradish peroxidase (hrp) granules. the cells studied were located in the trigeminal ganglia and were labeled after the injection of hrp solutions into single, maxillary, canine teeth. the tissue was examined with light field, dark field, and differential interference optical systems. the results indicated that differen ... | 1978 | 368347 |
| electron-microscopic demonstration of streptococcus mutans in 5-day-old experimental dental plaque using immunohistochemistry. | the present work described the distribution of horseradish peroxidase reaction product obtained after immunohistochemical staining of consecutive ultra-thin sections of 5-day-old experimental dental plaques using differently absorbed anti-s.mutans sobrinus, anti-s.mutans mutans, and normal rabbit lambda-globulins. it was found that an in situ identification of s.mutans subspecies sobrinus could be obtained using thoroughly absorbed anti-s.mutans sobrinus gamma-globulins and consecutive sections. ... | 1978 | 369295 |
| the effect of serotonin on the blood-brain barrier to proteins. | since increased concentration of serotonin (5-ht) has been demonstrated in areas of the brain exposed to ischemia and lesions, and since the elevation might be responsible for the enhanced permeability to proteins across cerebral vessels, studies were carried out to elucidate the effect of the amine, perfused through the cerebral ventricular system, on the transport of horseradish peroxidase (hrp) from blood to brain. the amounts of 5-ht were large (50--800 microgram per mouse). the permeability ... | 1978 | 290745 |
| effect of prostaglandin e2 on the permeability of the iris vessels to horseradish peroxidase in the rabbit. | prostaglandin e2 (0.5 microgram in 10 microliter 10% ethanol) was introduced into the anterior chambers of rabbit eyes. using the horseradish peroxidase method, it was shown under the electron microscope that the endothelial barrier of the iris vessels broke down. the peroxidase penetrated as far as the basis of the posterior epithelial cells, however, without entering their lateral intercellular spaces. the question of whether the effect of prostaglandins on the barrier was a direct effect or a ... | 1978 | 309739 |
| conjugation of poly-l-lysine to albumin and horseradish peroxidase: a novel method of enhancing the cellular uptake of proteins. | the carbodiimide-catalyzed conjugation of a 6700 molecular weight fragment of poly-l-lysine to radiolabeled human serum albumin or to horseradish peroxidase enhances the membrane transport of each protein into cultured mouse fibroblasts approximately 11- and 200-fold, respectively. at least 50% of the peroxidase activity remained after conjugation. trypsinization and carbamylation of the two conjugates demonstrates that the enhancement of their cellular uptake is related to their poly-l-lysine c ... | 1978 | 273916 |
| evidence for receptor-mediated binding of glycoproteins, glycoconjugates, and lysosomal glycosidases by alveolar macrophages. | alveolar macrophages have been shown to bind glycoproteins and synthetic glycoconjugates (neoglycorpoteins) that have mannose, n-acetylglucosamine, or glucose in the exposed, nonreducing position. galactose-terminal glycoproteins are not bound. binding of radiolabeled ligands to cells is nearly completely impaired by the presence of an excess of yeast mannan. binding is temperature sensitive and proceeds optimally at ph 7.0. prior treatment of the cells with trypsin severely decreases their capa ... | 1978 | 274729 |
| endocytosis of cholera toxin into neuronal gerl. | cholera toxin linked covalently by glutaraldehyde to horseradish peroxidase was incubated with cultured chicken sympathetic neurons at 4 degrees. cells were washed and brought to 37 degrees to permit endocytosis of bound toxin on plasma membranes. massive internalization of the ligand into vesicles and cisterns of the golgi--endoplasmic reticulum--lysosome (gerl) system was demonstrated by the cytochemical reaction for the enzyme. surface binding and subsequent endocytosis of the cholera toxin-- ... | 1978 | 275852 |
| the cellular response to topically applied horseradish peroxidase in the gingiva of normal and immunized dogs. i. a histometric and ultrastructural study. | 1978 | 147331 | |
| rat plasma clearance of horseradish peroxidase and yeast invertase is mediated by specific recognition. | 1978 | 202501 | |
| intracellular demonstration of growth hormone in human mammary carcinoma cells. | intracellular growth hormone has been demonstrated in human mammary carcinoma cells by using an unlabeled antibody enzyme technic with horseradish peroxidase and antihorseradish peroxidase complex. intense reaction was seen in both male and female mammary carcinoma cells. positive staining was observed in nuclei and cytoplasm and on cytoplasmic membranes. | 1978 | 204210 |
| intracellular horseradish peroxidase injection for correlation of light and electron microscopic anatomy with synaptic physiology of cultured mouse spinal cord neurons. | synaptic interactions between spinal cord neurons grown in dissociated cell culture were studied electrophysiologically, and presynaptic cells were subsequently injected by intracellular iontophoresis with horseradish peroxidase (hrp). following histochemical processing, injected cells were filled with dense reaction product which facilitated the light and electron microscopic identification of the individual physiologically typed neurons. this technique applied to neurons in monolayer culture a ... | 1978 | 209872 |
| ["functional and anatomical organization of associative" cortical areas 7, 21 and 22 in monkeys. a study of the cortical projections from the pulvinar by means of hrp tracing technique (author's transl)]. | the thalamic projections to the parietal and temporal associative areas (brodmann's areas 7, 21 and 22) have been revealed by means of the retrograde axonal transport of horseradish peroxidase (hrp). the medial pulvinar (pm) was found to send afferents to the three studied areas. labelled neurones of the pm appeared to be grouped in terms of their cortical target; indeed, neurones of origin of projection fibres to areas 7, 21 and 22 were respectively localized in the dorso-lateral, ventro-latera ... | 1978 | 211571 |
| comparison of an enzyme-linked immunosorbent assay for quantitation of rotavirus antibodies with complement fixation in an epidemiological survey. | the development of a micro-scale enzyme-linked immunosorbent assay (elisa) with horseradish peroxidase as the marker enzyme for the detection and measurement of human rotavirus antibodies is described. a semipurified preparation of the serologically related simian agent, sa-11 virus, was used as the antigen. test sera were reacted with antigen-sensitized wells in disposable poly-vinyl microplates. any attached antibody was detected by the addition of peroxidase-labeled anti-species immunoglobuli ... | 1978 | 212451 |
| binding of antibodies to acetylcholine receptors in electrophorus and torpedo electroplax membranes. | antisera against purified acetylcholine receptors from the electric tissues of torpedo californica and of electrophorus electricus were raised in rabbits. the antisera contain antibodies which bind to both autologous and heterologous receptors in solution as shown by an immunoprecipitation assay. antibodies in both types of antisera bind specifically to the postjunctional membrane on the innervated surface of the intact electroplax from electrophorus electric tissue as demonstrated by an indirec ... | 1978 | 344325 |
| the projection of the superior colliculus upon the inferior olivary complex of the cat: an autoradiographic and horseradish peroxidase study. | 1978 | 346175 | |
| organization of corticospinal neurons in the cat. | the cells of origin of the corticospinal tract of the cat were identified using the retrograde horseradish peroxidase (hrp) labeling technique. cortical neurons labeled from the spinal cord were confined to layer v and included large betz cells, as well as many smaller neurons of this layer. collections of 5-10 labeled neurons concentrated in areas of 300-500 micrometer diameter were observed, suggesting a columnar-type of organization of corticospinal neurons. injections of hrp into different s ... | 1978 | 348267 |
| the central location of the motor neurons to the stapedius muscle in the cat. | in two cats and 10 newborn kittens, retrograde transport of horseradish peroxidase was used to locate the motor neurons of the stapedius muscle. following injection of tracer into the muscle, labeled neurons were observed in the interface between the facial nerve nucleus and the caudal end of the lateral superior olivary nucleus. some marked neurons were also located dorsal and ventral to the facial nucleus. the number of labelled cells varied from a low of 34 to a high of 199. | 1978 | 348268 |
| immunocytological localization of estrogen in human mammary carcinoma cells by horseradish--anti-horseradish peroxidase complex. | tissues from 25 female and 2 male patients of mammary carcinoma were examined by the horseradish--anti-horseradish peroxidase complex to localize the presence of estrogen. carcinoma cells of 10 females and 2 male patients showed positive staining by this technique. the nucleus and cytoplasmic membrane showed the reaction. this method is useful in identifying the exact location of the hormones or hormone-like substances in the malignant cells and, perhaps, will be of great help in selecting the p ... | 1978 | 349272 |
| brain stem neurons projecting to neocortex: a hrp study in the cat. | the cortical projections of the brain stem were investigated in detail in the cat by means of the horseradish peroxidase (hrp) retrograde axonal transport. most of the cells providing ascending fibers to the neocortex were located in the pons (locus coerulus and related structures, central gray substance, dorsal tegmental nucleus, raphe nuclei, reticular nuclei); labeled neurons were also identified in the mesencephalon, mainly in the periaqueductal gray and in the nucleus linearis rostralis. th ... | 1978 | 350597 |
| cerebellar afferent projections from the vestibular nuclei in the cat: an experimental study with the method of retrograde axonal transport of horseradish peroxidase. | details of cerebellar afferent projections from the vestibular nuclei were investigated by the method of retrograde axonal transport of horseradish peroxidase (hrp) in the cat. the distribution of labeled cells in the vestibular nuclei following hrp injections in various parts of the cerebellum indicates that vestibular neurons in the medial and descending nuclei and cell groups f and x project bilaterally to the entire cerebellar vermis, the flocculus, the fastigal nucleus and the anterior and ... | 1978 | 350598 |
| characterization of various nervous tissues of the chick embryos through responses to chronic application and immunocytochemistry of beta-bungarotoxin. | beta-bungarotoxin (beta-bt) was applied to chick embryos at 3-day intervals beginning on the fourth day of incubation to investigate ultrastructurally the effects of chronically and massively applied beta-bt on various nervous tissues and muscles. on the twenty-first day of incubation, spinal cords of beta-bt treated embryos were conspicuously decreased in size. ventral root fibers dorsal root fibers, white matter, and motor neurons disappeared. although spinal ganglia and sympathetic trunk gang ... | 1978 | 350914 |
| histochemical differentiation of complex carbohydrates with variants of the concanavalin a-horseradish peroxidase method. | various treatments carried out prior to the concanavalin a-horseradish perioxidase (hrp) method have been found to affect the staining and have permitted differentiation of three main classes of complex carbohydrates in the rat alimentary tract. class i mucosubstances lose and class ii and iii paradoxically gain concanavalin a-horseradish peroxidase reactivity after periodate oxidation. class ii mucosubstances lose whereas class iii retain or increase their reactivity with a reduction step inter ... | 1978 | 351046 |
| a morphologic and permeability study of luminal smooth muscle cells after arterial injury in the rat. | in the carotid air drying model of arterial endothelial injury in the stressed rat, endothelium does not always regenerate over the zone of intimal thickening; instead, a layer of modified smooth muscle cells appears to form a temporary luminal surface. we examined the properties of these luminal smooth muscle cells in injured right carotid arteries from stressed rats at intervals up to 2 months by light, scanning electron, and transmission electron microscopy. before perfusion fixation, selecte ... | 1978 | 355725 |
| a simple device for pressure injections of horseradish peroxidase into small central neurones. | 1978 | 359104 | |
| the defined antigen substrate sphere system with direct immunohistoperoxidase for detection of soluble dengue antigen in sera of patients with dengue hemorrhagic fever. | the defined antigen substrate sphere system is a simple method for detecting antigen or antibody in the circulation. the technic is based on the coupling of antigen or antibody with sepharose 4b beads that have been activated by cyanogen bromide. in this study the activated beads were exposed to dengue antigen in the serum from a patient with dengue hemorrhagic fever and then stained with antidengue antibody conjugated with horseradish peroxidase. the positive reaction showed brown beads by ligh ... | 1978 | 360820 |
| enzyme immunoassay for total oestrogens in pregnancy plasma or serum. | we have developed an enzyme immunoassay for total oestrogens in pregnancy serum of plasma, using horseradish peroxidase (hrp) as the marker enzyme. a test combination consisting of an antiserum against oestriol-16/17-monosuccinyl-albumin and oestriol-16/17-monosuccinyl-hrp yielded a sensitive system, which reacted to approximately the same extent with oestrone, oestradiol, oestriol and their 16- and 17-conjugates. samples had to be diluted 1 to 10 to avoid interference of plasma factors with the ... | 1978 | 361293 |
| incorporation of rat histocompatibility (agb) antigens into liposomes, and their susceptibility to immune lysis. | the purification of detergent-solubilized rat histocompatibility antigens is described. the antigen may readily be incorporated into synthetic vesicles (liposomes) which appear to be unilamellar and between 0.1 microbeter and 0.2 micrometer in diameter. the addition of specific antibody leads to the agglutination and precipitation of the liposomes. an enzyme, horseradish peroxidase, may be incorporated into the trapped aqueous phase of the liposome, and its release by antibody and complement can ... | 1978 | 361419 |
| an improved enzyme immunoassay for progesterone in human plasma. | we describe an enzyme immunoassay for progesterone in which we use a progesterone-11alpha-hemisuccinyl-horseradish peroxidase conjugate as the "label" and an antiserum raised in rabbits to a progesterone-11alpha-hemisuccinyl-bovine serum albumin conjugate. in this assay, antibody-bound and free steroid are separated by using a second antibody precipitation procedure. the assay has a lower limit of sensitivity of 10 pg/assay tube and satisfies the usual criteria of specificity, precision, and acc ... | 1978 | 363304 |
| effect of histamine and methacholine on guinea pig tracheal permeability to hrp. | the effects of histamine, methacholine, and ether on the permeability of the respiratory mucosa to macromolecules were investigated employing a radioimmunoassay and histochemical techniques to monitor movement of horseradish peroxidase (hrp) from airway lumen to blood. we found that 0.08% of the dose of hrp instilled into guinea pig tracheas was present in the blood volume at 10 min, and plasma hrp levels increased at a rate of 0.0036% instilled dose/min thereafter. after inhalation challenge, s ... | 1978 | 365840 |
| caudate afferents from the retrorubral nucleus and other midbrain areas in the cat. | in order to clarify which midbrain areas project to the caudate nucleus (cd), horseradish peroxidase (hrp) was deposited in the cd of 13 cats. midbrain sections were reacted with diaminobenzidine and sometimes with cobaltous chloride to intensify the reaction, and then examined for retrograde labelling. afferents to the cd were found arising from the following ipsilateral midbrain areas: pars compacta, pars reticulata, and pars lateralis of the substantia nigra (sn), ventral tegmental area of ts ... | 1978 | 318187 |
| muscle spindle capillaries and capsule: a horseradish peroxidase study. | 1978 | 160105 | |
| a horseradish peroxidase study of the cerebellorubral pathway in the rat. | 1978 | 618740 | |
| uptake and fate of luminally administered horseradish peroxidase in resting and isoproterenol-stimulated rat parotid acinar cells. | the formation and fate of apical endocytic vesicles in resting and isoproterenol-stimulated rat parotid acinar cells were studied using luminally administered horseradish peroxidase (hrp) to mark the vesicles. the tracer was taken up from the lumen by endocytosis in small, smooth-surfaces "c"- or ring-shaped vesicles. about 1 h after hrp administration the vesicles could be found adjacent to the golgi apparatus. at later times hrp reaction product was localized in multivesicular bodies and lysos ... | 1978 | 618893 |
| early stages of uptake and transport of horseradish-peroxidase by cortical structures, and its use for the study of local neurons and their processes. | 1978 | 621289 | |
| human marrow erythropoiesis in culture: iii. ultrastructural and cytochemical studies of cellular interactions. | human erythroblasts cultured with a methylcellulose clonal assay technique were studied with ultrastructural and cytochemical methods. the intact colonies contained only erythroblasts at a similar stage of maturation, and no macrophages were identified within the colonies. the cultured erythroblasts demonstrated many of the morphologic features described in vivo. neutral and acid glycoconjugates identified on the plasmalemma stained in a similar way to that seen in vivo with a concanavalin a hor ... | 1978 | 624337 |
| immunoelectron microscopic localization of lipopolysaccharides in the cell wall of bacteroides oralis and fusobacterium nucleatum. | lipopolysaccharides (lps) have been extracted and purified from two anaerobic gram-negative bacteria: bacteroides oralis and tfusobacterium nucleatum. chemical analysis of the preparations showed a great proportion of neutral sugars, mainly glucose, in lps of b. oralis. in rabbits, lps of b. oralis induced both immunoglobulin m and g antibodies in contrast to lps of f. nucleatum, to which only immunoglobulin m antibodies were produced. an immunohistochemical method with horseradish peroxidase-la ... | 1978 | 624589 |
| the morphology of group ia afferent fibre collaterals in the spinal cord of the cat. | 1. the enzyme horseradish peroxidase (hrp) was injected into single ia muscle afferent fibres in anaesthetized cats. subsequent histochemistry allowed the morphology of the axons and their collaterals in the lumbosacral spinal cord to be determined. 2. fifteen ia axons were stained, four from medial gastrocnemius, four from lateral gastrocnemius-soleus and seven from muscles innervated by the posterior tibial nerve. all thirteen axons that could be traced into the dorsal roots bifurcated upon en ... | 1978 | 624988 |
| cortical projections of the thalamic mediodorsal nucleus in the rabbit. | the cortical projection of the thalamic mediodorsal nuclear complex (md) in the rabbit was mapped retrograde horseradish peroxidase and anterograde tritiated proline techniques. the projection field occupied the entire medial wall rostral to a mid corpus callosal level, wrapped around the frontal pole onto the lateral convexity and tailed off caudally on the dorsal bank of the rhinal sulcus. the projection of the lateral approximately one-half of md, the half which does not receive olfactory inp ... | 1978 | 626902 |
| the vestibular nuclei in the domestic hen (gallus domesticus). iv. the projection to the spinal cord. | horseradish peroxidase was injected at various levels of the spinal cord of the hen and the cells in the brain stem, labeled due to retrograde transport of the tracer enzyme, were mapped with particular reference to the vestibular nuclear complex. the deiters' nuclei project ipsilaterally to the spinal cord. the projection from the nucleus deiters ventralis reaches down to the lumbosacral spinal cord. the spinal projection from the nucleus deiters dorsalis is somatotopically organized. cells loc ... | 1978 | 630381 |
| the location of spinal projection neurons in the cerebellar nuclei (cerebellospinal tract neurons) of the cat. a study with the horseradish peroxidase technique. | the distribution of spinal projection neurons was studied in the cerebellar nuclei of the cat following injections of horseradish peroxidase (hrp) into the cervical, thoracic and lumbar cord. hrp-positive (labeled) neurons were found in the medial (fastigial) and the posterior interpositus nuclei on the side contralateral to the cervical injection, being most numberous in cases with injections between the c2 and the c3 segments. in the medial nucleus (m) labeled neurons were distributed in the c ... | 1978 | 630384 |
| postnatal development of rat cerebellum: massive and transient accumulation of concanavalin a binding glycoproteins in parallel fiber axolemma. | modifications of protein-bound sugars during postnatal development of rat cerebellum were studied. glycoprotein-bound mannose accumulates, in the particulate fractions, at an earlier age than the bulk of glycoprotein sugar. this corresponds to a transient and massive accumulation of glycoproteins which bind to concanavalin a (con a). these glycoproteins were localized by using fluorescent con a and the horseradish peroxidase-con a method. cerebellar white matter and the molecular layer bind mass ... | 1978 | 630386 |
| visual corticopontine input to the paraflocculus: a combined autoradiographic and horseradish peroxidase study. | 1978 | 630397 | |
| uptake of horseradish peroxidase from csf into the choroid plexus of the rat, with special reference to transepithelial transport. | protein uptake from cerebral ventricles into the epithelium of the choroid plexus, and transport across the epithelium were studied ultrastructurally in rats. horseradish peroxidase (hrp, mw 40,000) was used as protein tracer. steady-state ventriculo-cisternal perfusion with subatmospheric pressure (-10cm of water) in the ventricular system was applied. hrp dissolved in artificial csf was perfused from the lateral ventricles to cisterna magna for various times, and ventriculo-cisternal perfusion ... | 1978 | 630593 |
| afferent projections to the rat locus coeruleus as determined by a retrograde tracing technique. | afferent connections to the rat locus coeruleus (lc), which contains exclusively noradrenergic neurons, have been traced using the technique of retrograde transport of horseradish peroxidase (hrp). in order to ensure accurate placement of adequate amounts of hrp in the lc, a microiontophoretic delivery technique coupled with single cell recording was employed. the use of electro physiological "landmarks" as aids in placing the injections is described. following hrp injections into the lc, forebr ... | 1978 | 632368 |
| association and commissural fiber systems of the olfactory cortex of the rat. | the association and commissural fiber systems arising in the olfactory cortical areas caudal to the olfactory peduncle (the piriform cortex, nucleus of the lateral olfactory tract, anterior cortical nucleus of the amygdala, periamygdaloid cortex and entorhinal cortex) have been studied utilizing horseradish peroxidase as both an anterograde and a retrograde axonal tracer. in the piriform cortex two sublaminae within layer ii (iia and iib) layer iii have been found to give rise to distinctly diff ... | 1978 | 632378 |
| increased permeability to horseradish peroxidase across cerebral vessels, evoked by electrically induced seizures in the rat. | under normal conditions a slight vesicular transfer of intravenously injected horseradish peroxidase (hrp) occurs across the endothelium of cerebral vessels, especially short segments of arterioles. the vesicular transport can be notably increased by chemically induced acute hypertension. in the present investigation 4 groups of animals received hrp, and the permeability of the cerebral endothelium was studied semimacroscopically, light microscopically and electron microscopically. the rats in g ... | 1978 | 636839 |
| cells of origin of the spino-facial pathway in the cat: a horseradish peroxidase study. | 1978 | 638754 | |
| afferent projections to the central nucleus of the inferior colliculus in the rat. | the afferent projections to the inferior colliculus of the rat were studied using the method of retrograde transport of horseradish peroxidase (hrp). following large injections of hrp into the central nucleus, cells within the cochlear nuclei, superior olivary complex and auditory cortex were stained. within the contralateral dorsal cochlear nucleus, fusiform cells were heavily labeled. giant cells were also labeled in deeper layers. in the contralateral ventral cochlear nucleus, virtually all m ... | 1978 | 638786 |
| observations on the afferent and efferent connections of the avian isthmo-optic nucleus. | the efferent and afferent connections of the avian isthmo-optic nucleus (ion) were studied using light microscopic techniques. injections of [3h]proline into the nucleus resulted in labeling of centrifugal endings in the retina at the junction of the inner plexiform layer and inner nuclear layer, but produced no other transported label to any thalamic or mesencephalic nucleus. the origin of the tectal afferents to the ion was demonstrated by means of injections of [3h]proline into the most super ... | 1978 | 638787 |
| projections of the ascending somesthetic pathways to the cat superior colliculus visualized by the horseradish peroxidase technique. | projections of the somesthetic ascending pathways to the superior colliculus (sc) of the cat have been demonstrated by means of the horseradish peroxidase (hrp) tracing technique. following hrp injection in one sc, marked neurons were found in the contralateral dorsal horn and in the contralateral dorsal column nuclei, mainly the cuneate nucleus. moreover, in the contralateral trigeminal complex, the sub-nucleus caudalis of the spinal nucleus of the vth nerve, and the subnucleus oralis plus the ... | 1978 | 639909 |
| afferent fiber connections from lower brain stem to hypothalamus studied by the horseradish peroxidase method with special reference to noradrenaline innervation. | attempts were made to determine the afferent projections to the anterior hypothalamus including the preoptic area from the lower brain stem by means of the horseradish peroxidase method combined with monoamine oxidase staining to identify noradrenaline (na) neurons. in addition to this technique, a histofluorescence analysis was performed. na fibers in the medial part of the anterior hypothalamus were mainly supplied by a1 and a2 na neuron groups, while the lateral part and periventricular zone ... | 1978 | 639912 |
| a ventral lateral geniculate nucleus projection to the dorsal thalamus and the midbrain in the cat. | retrograde tracing experiments using horseradish peroxidase (hrp) have been utilized for demonstrating the origin of efferent projections of the ventral lateral geniculate nucleus (lgnv) in the cat. hrp-positive cells identifiable as origins of thalamic projections were found in lgnv after injections of hrp into the lateral central intralaminar nucleus. the labeled cells appeared concentrated in the medial part of the internal division of lgnv, consisting of medium-sized multipolar cells. contra ... | 1978 | 639913 |
| horseradish peroxidase application to the protein transport investigations and alkaline phosphatase ultralocalization at the kidney barrier level. | horseradish peroxidase was used to the precise investigation of low molecular protein transport across the kidney barrier. simultaneously on the parallel material alkaline phosphatase activity was revealed, as this enzyme is connected with the transport processes through cell membranes. the applied reaction diaminebenzidine has shown infiltration spots for low molecular protein at the glomercular barrier and visualized tubular reabsorption of peroxidase in proximal convoluted tubule. alkaline ph ... | 1978 | 640511 |
| ultrastructural localization of thermoplasma acidophilum surface carbohydrate by using concanavalin a. | surface carbohydrate, presumably the lipopolysaccharide, of thermoplasma acidophilum was visualized by means of the concanavalin a, horseradish peroxidase, and diaminobenzidine cytochemical staining procedure. | 1978 | 641014 |
| the organization of olivo-cerebellar projections in the opossum, didelphis virginiana, as revealed by the retrograde transport of horseradish peroxidase. | the retrograde transport of horseradish peroxidase was utilized to map olivo-cerebellar projections in the virginia opossum. the spinal cerebellum (anterior lobe, paramedian lobule and pyramis) receives input from several separate regions in the dorsal accessory nucleus, the medial accessory nucleus and portions of the principal nucleus. evidence is present for a topographical organization whereby specific regions of the olive project to restricted longitudinal zones. the visual-auditory region ... | 1978 | 641222 |
| the vestibular nuclei in the domestic hen (gallus domesticus) iii. ascending projections to the mesencephalic eye motor nuclei. | following injections of horseradish peroxidase in the oculomotor and the trochlear nuclei in the hen, the occurrence of labeled cells was plotted in the vestibular nuclei. the majority of labeled cells was localized in the superior, the medial, and the tangential nucleus. within the superior nucleus the cells were found mainly caudally, extending medially and ventrally in central areas. in the medial nucleus labeled cells were localized exclusively in its rostral half, mainly in ventrolateral re ... | 1978 | 641224 |
| superior colliculus connections with the extraocular motor nuclei in the cat. | direct and indirect projections from the cat superior colliculus to the extraocular motor nuclei were studied using the orthograde autoradiographic tracing method, the retrograde horseradish peroxidase technique, and golgi methods. the results show that the superior colliculus projects to the central gray matter directly overlying the oculomotor complex. this projection arises almost entirely from the rostral third of the colliculus, and it terminates most heavily over the rostral half of the oc ... | 1978 | 641226 |
| the morphology of the spinal cord efferent and afferent neurons contributing to the ventral roots of the cat. | horseradish peroxidase was applied to proximal ventral roots of the coccygeal and sacral spinal cord of cats. subsequent histochemical reaction resulted in extensive staining of spinal cord neurons that had processes in the ventral roots. this procedure was used to study four issues concerning ventral root neurons. (1) extensive transverse dendritic arborizations were revealed for large and small neurons presumed to be alpha and gamma motoneurons respectively. dendrites from these neurons were f ... | 1978 | 641227 |
| oogenesis in xenopus laevis (daudin). vi. the route of injected tracer transport in the follicle and developing oocyte. | in xenopus laevis, vitellogenin (the yolk precursor) is synthesized in the liver and transported via the circulatory system to the ovary. in order to reach developing oocytes where it is sequestered, it must exit the circulatory system and traverse several follicular tissue layers including the theca, the follicle cell layer, and the vitelline envelope. this study demonstrates this pathway by means of electron-opaque tracers, and follows the fate of heterologous macromolecules after their incorp ... | 1978 | 641487 |
| alterations in rat jejunal permeability to a macromolecular tracer during a hyperosmotic load. | the effects of intraluminal hyperosmolality on the intestinal permeability of a macromolecular tracer, horseradish peroxidase, was assessed in rats by light and electron microscopic cytochemistry. after 60 minutes of an intraluminal hypertonic perfusion of 600 mosm mannitol, horseradish peroxidase was demonstrable in the intercellular spaces between adjacent absorptive epithelial cells of jejunal villi, along the microvillar brush border, in numerous pinocytotic vesicles, in multivesicular bodie ... | 1978 | 642452 |
| afferents to brain stem nuclei (brain stem raphe, nucleus reticularis pontis caudalis and nucleus gigantocellularis) in the rat as demonstrated by microiontophoretically applied horseradish peroxidase. | using a retrograde tracer technique with microiontophoretically applied horseradish peroxidase (hrp), afferent projections to the brain stem raphe nuclei (br, raphe magnus, pallidus and obscurus) and to two adjacent reticular nuclei, nucleus reticularis pontis caudalis (nrpc) and nucleus gigantocellularis (ngc) were identified. the most striking difference between the afferent projections to the br and the adjacent nuclei as determined by this method is that afferents to the br originate primari ... | 1978 | 646855 |
| preparation of protein conjugates via intermolecular disulfide bond formation. | conjugates of two unlike proteins can be prepared via the intermolecular disulfide interchange reaction, namely, protein a containing thiol groups reacts with protein b containing 4-dithiopyridyl groups to yield a conjugate with the release of 4-thiopyridone. thiol groups can be introduced into proteins upon amidination with methyl 3-mercaptopropionimidate ester or 2-iminothiolane, and 4-dithiopyridyl groups can be introduced into proteins with these same reagents in the presence of 4,4'-dithiod ... | 1978 | 646998 |
| prosencephalic pathways related to the paleostriatum of the pigeon (columba livia). | afferent connections of the avian paleostriatal complex were traced by means of anterograde and retrograde transport of horseradish peroxidase (hrp). the paleostriatum augmentatum (pa), a cell field comparable to mammalian caudate nucleus and putamen, was found to receive projections from a distinct population of elencephalic neurons in the temporal-parietal-occipital (tpo) and lateral cortical (cdl) areas of the neostriatum. tpo neurons, in turn, were found to receive projections from the contr ... | 1978 | 647399 |
| multiple branching of cerebellar efferent projections in cats. | the retrograde labeling of neurons in the deep cerebellar nuclei with horseradish peroxidase was used to compare the morphological characteristics of neurons in the dentate and interposed nuclei projecting in the cerebellothalamic, cerebello-olivary, and cerebellar nucleocortical pathways. the results from these studies demonstrated that cerebellothalamic and nucleocortical projections from the dentate and interposed nuclei originate from similar populations of spindle- and multipolar-shaped neu ... | 1978 | 648599 |
| [degradation of the parathyroid hormone in the kidney and liver (author's transl)]. | the cleavage of the parathyroid hormone has been reported to take place in various peripheral tissues, especially in the kidney and liver. in order to claify the mechanism of such a degradation, bovine pth (bpth 1--84) and its synthetic n-terminal peptide (bpth 1--34) labelled with 125i by the chroramine-t method (125i-bpth 1--84) and (125i-bpth 1--34) or labelled with horseradish peroxidase pox-125i-bpth 1--84 and pox 125i-bpth 1 34) were used to study the disappearance from the blood stream an ... | 1978 | 648683 |
| the permeability of a keratinizing squamous epithelium in culture. | horseradish peroxidase or lanthanum was applied to the surface of keratinized oral epithelium growing in tissue culture and the extent of penetration of these substances examined with the electron microscope. both tracer substances penetrated between the superficial keratinized squames of the tissue, the lanthanum reaching the basal cell layer and the peroxidase diffusing to within 3--8 cells of the basal layer. the permeability of the keratinized layer in this epithelium is in contrast to the s ... | 1978 | 649491 |
| neuronal ceroid-lipofuscinosis. studies of granulocyte enzyme activities. | neuronal ceroid-lipofuscinosis is characterized by pigmentary degeneration of the retina, psychomotor degeneration, epilepsy and intracellular deposition of ceroidlipofuscin. recent reports have suggested that deficiency of peroxidase is the basic genetic defect. however, deficiency of myeloperoxidase could be demonstrated in some but not all patients; this deficiency was noted only when p-phenylenediamine (ppd) was used as hydrogen donor and could not be confirmed with guaiacol. we found that h ... | 1978 | 650251 |
| cytoarchitecture and thalamic connectivity of third somatosensory area of cat cerebral cortex. | 1. the third somatosensory area (siii) was identified in the cat cerebral cortex by the recording of surface potentials evoked by deflection of a single contralateral mystacial vibrissa. a small amount of tritiated leucine was then injected at the center of the focus of evoked activity and, after a suitable survival period, the brain was prepared for autoradiography. 2. as defined by the presence of an autoradiographic injection, the siii focus lay in a cytoarchitectonic field characterized in p ... | 1978 | 650267 |
| comparison of methods for demonstrating retrogradely transported horseradish peroxidase in neurones [proceedings]. | 1978 | 650512 | |
| morphology and organization of axon collaterals from afferent fibres of slowly adapting type i units in cat spinal cord. | 1. the morphology of the collaterals of single axons innervating type i slowly adapting receptors was studied by using the intra-axonal injection of the enzyme horseradish peroxidase in anaesthetized cats. the axons were impaled near their dorsal root entrance zone in the lumbosacral cord. the morphology was revealed by subsequent histochemistry. 2. thirteen type i axons were stained, nine with receptors in the hairy skin and four with receptors in the glabrous foot pad skin. twelve axons could ... | 1978 | 650514 |
| studies of pulmonary innervation in the rat using horseradish peroxidase as a neuronal marker [proceedings]. | 1978 | 650517 | |
| horseradish peroxidase uptake into the rat choroid plexus epithelium, with special reference to the lysosomal system. | 1978 | 650732 | |
| glomerular filtration of proteins: clearance of anionic, neutral, and cationic horseradish peroxidase in the rat. | glomerular permeability to horseradish peroxidase, a protein slightly smaller than rat albumin but similar in shape, was studied in wistar-furth rats by using a purified neutral isozyme (hrp; molecular radius, a(e) = 29.8 a) as well as an anionic succinyl-derivative (shrp a(e) = 31.8 a) and a cationized enzyme (chrp, a(e) = 30 a). the clearance rate of the proteins was determined over a 20-min period using the amounts of enzyme actually filtered (i.e., protein in the urine and protein reabsorbed ... | 1978 | 651127 |
| ascending and intrinsic projections of the superior olivary complex in the cat. | the ascending and intrinsic projections of the superior olivary complex (so) in the cat were investigated by injection of 3h-leucine and horseradish peroxidase (hrp) in so and the inferior colliculus (ic), respectively. a topically arranged projection was demonstrated from the nucleus of the trapezoid body (ntb) to the ipsilateral lateral superior olivary nucleus (lso) wiht a lesser connection in the opposite direction. the medial superior olivary nucleus (mso) has a strictly ipsilateral project ... | 1978 | 658183 |
| selective binding, uptake, and retrograde transport of tetanus toxin by nerve terminals in the rat iris. an electron microscope study using colloidal gold as a tracer. | a series of specific macromolecules (tetanus toxin, cholera toxin, nerve growth factor [ngf], and several lectins) have been shown to be transported retrogradely with high selectivity from terminals to cell bodies in various types of neurons. under identical experimental conditions (low protein concentrations injected), most other macromolecules, e.g. horseradish peroxidase (hrp), albumin, ferritin, are not transported in detectable amounts. in the present em study, we demonstrate selective bind ... | 1978 | 659508 |
| anatomy of germinal centers in mouse spleen, with special reference to "follicular dendritic cells". | lymphocyte proliferation in germinal centers (gc's) is thought to be triggered by antigen retained extracellularly on the surface of special "dendritic" cells. the anatomy and function of these cells have not been studied directly or in detail. we therefore examined mouse spleen gc's developing in response to sheep erythrocyte stimulation. we found that distincitve "follicular dendritic cells" (fdc's) were present in both the gc and adjacent mantle region of secondary follicles. the large, irreg ... | 1978 | 659510 |
| organization and development of the brain stem auditory nuclei of the chicken: primary afferent projections. | the pattern of primary auditory projections to the brain stem of young chickens was investigated using terminal degeneration methods and orthograde transport of horseradish peroxidase (hrp) or tritiated amino acid. of particular interest was the question of whether nucleus laminaris (nl) receives primary afferents. a study of silver-stained degeneration pattersn in nucleus magnocellularis (nm) and nl at three intervals following unilateral interruption of the cochlear nerve revealed that by 48 h ... | 1978 | 659669 |
| properties of the enteric nervous system: limitation of access of intravascular macromolecules to the myenteric plexus and muscularis externa. | the possible presence of a blood-myenteric plexus barrier similar to the blood-nerve and blood-brain barriers was investigated. the myenteric plexus was found to be an enclosed tubular structure incompletely surrounded by a sheath of supporting cell processes. capillaries do not enter the plexus. the capillaries which supply the myenteric layer differ in structure from capillaries of other layers of the gut and are non-fenestrated. tracers, evans blue labeled albumin or horseradish peroxidase, r ... | 1978 | 659670 |
| catecholamine innervation of the basal forebrain. iv. topography of the dopamine projection to the basal forebrain and neostriatum. | in this study the location of dopamine (da) neuron perikarya in the rostral mesencephalon of the rat was determined using the glyoxylic acid fluorescence histochemical technique. subsequently the topography of the projection of these mesencephalic neurons on the basal forebrain and striatum was analyzed using the anterograde transport-autoradiographic tracing method and the retrograde transport-horseradish peroxidase (hrp) technique. the results of these anatomical studies were correlated with t ... | 1978 | 659674 |
| the morphologic basis of proteinuria in experimental chronic serum sickness glomerulonephritis. a light and electron microscopic study using horseradish peroxidase and ferritin as tracers. | the morphologic basis of proteinuria in experimental chronic serum sickness glomerulonephritis in rabbits was studied by light and electron microscopy using horseradish peroxidase (effective radius 30 a; mol. wt. 40,000) and ferritin (effective radius 60 a; mol. wt. 480,000) as protein tracers. it was found that more ferritin, but paradoxically, less horseradish peroxidase gained access to the urinary space. observations made by electron microscopy appeared to indicate a decreased permeability o ... | 1978 | 662050 |
| behavioral and anatomical consequences of small intrastriatal injections of kainic acid in the rat. | the effects of bilateral injections of kainic acid into the anteromedial neostriatal region were examined behaviorally and anatomically in two groups of rats. behaviorally, kainic acid injections resulted in a severe impairment of delayed alternation retention, while the ability for visual discrimination remained unaffected. anatomically it was found that axons traversing the injected area remain able to transport horseradish peroxidase. furthermore, histological examinations of the injected reg ... | 1978 | 667628 |