Publications

localization of parabrachial nucleus neurons projecting to the thalamus or the amygdala in the cat using horseradish peroxidase.		1979	428513
morphologic study of the action of lymphoid tissue extracts in peroxidase arthritis.	by means of modelling arthritis by horseradish peroxidase it was possible to reproduce a process corresponding to immune synovitis. a considerable reduction of immunomorphological phenomena in joints, lymphoid tissue, heart, liver and kidneys was favoured by application of lymphoid tissue extracts. comparison between the extracts of spleen and lymph nodes showed a more pronounced effect of spleen extract.	1979	428522
sympathetic neurons in the cat spinal cord projecting to the stellate ganglion.	horseradish peroxidase (hrp) was injected into the stellate ganglia of anesthetized cats. after a 2-day survival time the spinal cord was processed for the hrp reaction to study the distribution of the preganglionic neurons which project to the stellate ganglia. hrp-labeled neurons in the cord were located exclusively on the side ipsilateral to the injected stellate ganglia and were concentrated in four distinct areas: (1) intermediolateral cell column (78.2%), (2) lateral funiculus (18.5%), (3) ...	1979	429616
the lateral cervical nucleus in the cat: anatomic organization of cervicothalamic neurons.	the morphology of the lateral cervical nucleus (lcn) and the organization of the cervicothalamic projection neurons were studied in cats which had received thalamic injections of horseradish peroxidase (hrp). the boundaries of the lcn were defined following very large thalamic (hrp injections. roughly 92-97% of lcn cells project contralaterally to thalamus; an additional 1.5% project ipsillaterally. computer-assisted measurements of perikaryal areas demonstrated that there are two sizes of lcn c ...	1979	429620
localization of neurons in the rat superior cervical ganglion that project into different postganglionic trunks.	horseradish peroxidase (hrp) was used to determine whether neurons in the rat superior cervical ganglion (scg) are localized in regions of the ganglion as a function of the postganglionic trunk they utilize. in separate experiments, each of the two major postganglionic trunks was cut 1-3 mm from the scg and solid hrp was applied to the cut end proximal to the ganglion. the results demonstrated that the cell bodies of neurons whose axons project out the internal carotid nerve (icn) were located p ...	1979	429622
submandibular glandular permeability to blood-borne horseradish peroxidase in dogs, before and after obstructing secretion [proceedings].		1979	430395
endocytosis in glial cells (pituicytes) of the rat neurohypophysis demonstrated by incorporation of horseradish peroxidase.		1979	431821
patterns of subcortico-cortical projections as revealed by somatopetal horseradish peroxidase tracing.		1979	431824
identification of cerebellar corticovestibular neurons retrogradely labeled with horseradish peroxidase.		1979	431827
direct projection of catecholamine (presumably dopamine)-containing neurons from hypothalamus to spinal cord.	a procedure for combining catecholamine (ca) and horseradish peroxidase (hrp) histochemistry was used to demonstrate that some of the catecholamine-containing cells in the rabbit dorsal hypothalamus project directly to the thoracic spinal cord. these cells, which are probably dopaminergic, may be involved in the integration of pituitary hormonal secretion and peripheral autonomic nervous regulation.	1979	431883
cerebellar afferents from the nucleus of the solitary tract.	the cerebellar afferents from the nucleus of the solitary tract were studied in cat by means of retrograde axonal transport of horseradish peroxidase. labelled cells occurred bilaterally in the nucleus of the solitary tract following injections in various folia of the cerebellar vermis and in the flocculus (the positive cases are shown in fig. 1a). injections in the anterior lobe vermis labelled cells in the caudal part of the nucleus, injections in the posterior vermis labelled cells in the ros ...	1979	431884
the fine structure of neurones and cellular relationships in the abducens nucleus in the cat.	light and electron microscope studies of the abducens nucleus in the cat have disclosed two populations of neurones: large neurones 25 to 75 micrometer in diameter and small neurones 10 to 25 micrometer in diameter. characteristic of the large neurones are a highly developed granular endoplasmic reticulum and a large number of axo-somatic synapses. the small neurones have a poorly developed granular endoplasmic reticulum and comparative by few axo-somatic synapses. injection of horseradish perox ...	1979	436996
development of spinal motoneuron innervation of the upper limb muscle in the rat.	horseradish peroxidase was injected into the biceps brachii muscle of rats at various stages of development, from 10 days to 50 weeks. the retrogradely labeled neurons were found in the ipsilateral ventro-lateral column of the cervical cord, c4--c8, of all stages studied, but the number of labeled neurons decreased according to exponential curve as the age advanced. a striking finding was that the contralateral ventral horn cells were also labeled in the 10- and 14-day-old rats.	1979	436997
uptake of exogenous protein by supraependymal cells of the feline area postrema.	supraependymal cells occurring on the surface of the feline area postrema were examined for phagocytic ability. it was shown that they could ingest exogenous horseradish peroxidase that was experimentally introduced into the brain ventricular system. the cells thus bear functional as well as ultrastructural attributes of macrophages, similar to those found in the third ventricle and subarachnoid space.	1979	437052
the uptake of horseradish peroxidase by damaged autonomic nerves in vitro.	the uptake of horseradish peroxidase by damaged autonomic nerves was studied in vitro. large amounts of the enzyme penetrated the axons in a diffuse (nonvesicular) form. this occurred within 90 minutes of application of the enzyme. twenty four hours after application of the enzyme diffuse peroxidase was still present in the axoplasm, but it was 'diluted' as a result of great axonal swelling. pinocytotic uptake of the enzyme was observed both 90 minutes and 24 hours after application of the enzym ...	1979	438097
glomerular permeability of macromolecules. effect of molecular configuration on the fractional clearance of uncharged dextran and neutral horseradish peroxidase in the rat.	molecular parameters other than size and charge are likely to influence the filtration of macromolecules across the glomerular filter. we have studies, therefore, the glomerular permeability of macromolecules with widely different molecular configuration such as horseradish peroxidase, a plant glycoprotein with an isoelectric point in the physiologic ph range, and dextran, an uncharged sugar polymer of d-glucopyranose. simultaneous fractional clearances were determined for both test macromolecul ...	1979	438331
the morphological and physiological properties of a regenerating synapse in the c.n.s. of the leech.	regeneration of an electrical synapse between particular interneurons in the medicinal leech was traced physiologically and morphologically using intracellular recording the horseradish peroxidase (hrp) injection. the synapse between s-cell interneurons lies in the connective midway between segmental ganglia, so crushing near one ganglion severs only one s-cell's axon. the severed distal stump remains connected to the adjacent uninjured s-cell and continues for weeks to conduct impulses. the inj ...	1979	438368
opening of tight junctions in cerebral endothelium. i. effect of hyperosmolar mannitol infused through the internal carotid artery.	infusion of 1.8 m mannitol solution into the internal carotid artery of wistar rats allows horseradish peroxidase (hrp) to cross cerebral vascular endothelium via intercellular pathways. this was made evident by density gradients of hrp observed in consecutive tight junctional compartments following in vivo administration, and by the passage of colloidal lanthanum through junctional membrane appositions of glutaraldehyde fixed cerebral endothelium. three hours after mannitol infusion, small non- ...	1979	438370
opening of tight junctions in cerebral endothelium. ii. effect of pressure-pulse induced acute arterial hypertension.	acute arterial hypertension was produced in male wistar rats by pressure pulse through the right internal carotid artery. the pressure pulse was induced by infusion of physiological saline as a bolus, at a rate of 0.63 ml per second by syringe pump. evans blue (eb) was used to visualize the areas of blood-brain barrier opening. intravenously injected horseradish peroxidase (hrp) was used to study the ultrastructural basis of permeability changes in cerebral endothelium. eb outlined circumscribed ...	1979	438371
a microplate immunoenzyme assay for anti-influenza antibodies.	an enzyme-linked immunosorbent assay (elisa) using horseradish peroxidase is described for the detection and quantitation of anti-influenza virus antibodies. compared with complement fixation and hemagglutination inhibition tests, elisa is far superior with respect to sensitivity and reliability. non-specific viral inhibitors present in sera do not affect the titer in elisa. its sensitivity, close to that of radioimmunoassay permits detection of small amounts of antibodies in pulmonary secretion ...	1979	438513
histochemical identification of acetylcholinesterase in dopaminergic nigrostriatal neurons.	by combining histochemical procedures, evidence was obtained in the rat that acetylcholinesterase (ache) is contained within perikarya of dopaminergic substantia nigra cells which project to the caudoputamen. nigrostriatal neurons were identified by retrograde transport of horseradish peroxidase (hrp). simultaneous incubation of substantia nigra sections for the presence of ache and hrp demonstrated both reaction products in the same cell bodies. the catecholaminergic nature of these cells was d ...	1979	438805
early and late mechanisms of increased vascular permeability following experimental cerebral infarction.	cerebral infarction was produced in rats by a combination of transient unilateral common carotid artery occlusion and systemic hypoxia. horseradish peroxidase (hrp) and evans blue were given 5 minutes prior to sacrifice to assess the integrity of the blood-brain barrier (bbb) at 1 minute, 30 minutes, and 2 hours following the ischemic insult. there was immediate permeability to hrp in the early (1 minute and 30 minutes) post-ischemic period, whereas, evans blue was not seen until the late (1.5 t ...	1979	438863
[horseradish peroxidase labeled neurons of the ventrobasal complex of the thalamus projecting to the somatosensory zone i of the cat cerebral cortex].	the morphology and topography of the neurons in the ventro-basal complex (vb) projecting to the somatic sensory cortex (si) were studied by the method of the retrograde axonic transport of horseradish peroxidase (hrp). different types of hrp-labelled cells were found in this structure. the concentration of labelled cells was the greatest in the central part of the vb. the most peculiar feature in the distribution of hrp-positive neurons was their organization in two large populations in the dors ...	1979	440485
inhibition of the discharge of endocytosed protein from phagosomes into lysosomes in hepatoma cells exposed to dimerized ribonuclease a.	the mechanism of the cytostatic action of dimerized ribonuclease a toward cultured hepatoma cells was investigated. a decrease in mitotic index, modifications of adsorptive properties of the pericellular membrane and inhibition of the degradation of two different proteins taken up by endocytosis are the first cell functions to be affected by the dimer. this effect on protein digestion is not due to an inhibition of proteolytic enzymes. the intracellular localization of exogenous protein and of r ...	1979	444221
intercellular junctions and the development of the blood-brain barrier in manduca sexta.	in early embryonic development of the tobacco horn moth no blood-brain barrier is present, as shown by the unimpeded entry of exogenous tracers into the nervous system. however, later on, just before hatching, lanthanum and horseradish peroxidase (hrp) are unable to move inwardly beyond the level of the perineurium, which is the morphological site of the blood--brain barrier in the adult moth, as well as in other insects. freeze-fracture studies indicate that in the early embryo, 10 nm particles ...	1979	445142
quantitative histological study of spinal afferent innervation on the ventral surface of the cat stomach by horseradish peroxidase (hrp) method.	retrograde axonal transport of horseradish peroxidase (hrp) was applied to the ventral surface of the cat stomach. we investigated the number, size and distribution of hrp-positive cells in spinal ganglia. the unexpected finding was the wide distribution of these cells from t3 down to l3. this would result in a diffuse pattern of referred pain.	1979	446627
prefrontal cortex of the cat: evidence for an additional area.	direct projections from the mediodorsal nucleus of the thalamus to ventral parts of the insular region of the cat's cortex were demonstrated by using the horseradish peroxidase technique.	1979	446633
abnormal retino-geniculate and geniculo-cortical pathways in several genetically distinct color phases of the mink (mustela vison).	several genetically distinct color phases of mink, which all show an abnormal reduction of pigment in the retinal pigment epithelium and which also show abnormalities of the retinofugal pathways, have been studied. autoradiographic methods have been used to demonstrate the retino-geniculate pathways, and retrograde degeneration or the retrograde transport of horseradish peroxidase has been used for the geniculo-cortical pathways. the retino-geniculate abnormality is mild in some of the color pha ...	1979	447875
an hrp and autoradiographic study of the projection from the cerebellar cortex to the nucleus interpositus anterior and nucleus interpositus posterior of the cat.	the recently developed anatomical techniques of retrograde transport of the enzyme horseradish peroxidase (hrp), anterograde transport of tritiated amino acid, and intracellular injections of hrp were used to study the organization of the corticonuclear projection to the nucleus interpositus anterior (nia) and the nucleus interpositus posterior (nip) of the cat. injections of hrp into the nia and the nip revealed that the major areas of the cortex which provided afferents to these two nuclei wer ...	1979	447879
reexamination of the dorsal root projection to the spinal dorsal horn including observations on the differential termination of coarse and fine fibers.	primary afferent fibers in the lumbar, sacral, and caudal spinal segments of several mammals (rat, cat, monkey) were stained by applying horseradish peroxidase to the proximal part of cut dorsal rootlets and reacting the tissue histochemically after several hours of survival. the stained fibers' pattern of termination in the dorsal horn was similar in all three species, with many bouton-like enlargements in the ipsilateral marginal zone, substantia gelatinosa, and nucleus proprius, as well as a ...	1979	447880
morphological features of functionally defined neurons in the marginal zone and substantia gelatinosa of the spinal dorsal horn.	functional characteristics of spinal neurons located in the marginal zone (lamina i) and substantia gelatinosa (lamina ii) were compared to their structural features by intrcellularly staining the source of unitary potentials with horseradish peroxidase (hrp) in unanesthetized, spinal cats. the responses of postsynaptic units to graded electrical volleys in intact dorsal roots and to physiological stimulation revealed that the peripheral excitatory input to neurons of the region is dominated by ...	1979	447881
structure of physiologically identified x and y cells in the cat's lateral geniculate nucleus.	horseradish peroxidase injected into 18 single, physiologically identified geniculate x and y cells permitted a detailed morphological correlate to be determined for the physiological properties of each neuron. class 1 morphological characteristics were associated with y cells, class 3 with x cells, and class 2 structural traits were seen in both physiological types.	1979	451559
quantitative estimate of pinocytosis in experimental acute hypertension.	cerebral cortical arterioles in focal neocortical areas develop increased permeability to plasma proteins and protein tracers in experimental hypertensive encephalopathy. the mechanism underlying this increased permeability has been the subject of several studies. in our previous studies of angiotensin-induced acute hypertension, pinocytosis appeared to be the principal mechanism for the increased blood-brain barrier (bbb) permeability observed. in the present study pinocytotic activity was asse ...	1979	452852
a study of the perineurial diffusion barrier of a peripheral ganglion.	the perineurial diffusion barrier to horseradish peroxidase (hrp) and ferritin was investigated in superior cervical ganglia of rats and mice. the ganglion was surrounded by a delicate epineurium and 2-5 perineurial lamellae joined by zonulae occludentes and desmosomes. following local application of tracers the animals were killed after 5, 30, and 60 min and the distribution of hrp and ferritin was studied by light and electron microscopy. the inner layers of the ganglionic perineurium prevente ...	1979	452854
bactericidal effect of cysteine exposed to atmospheric oxygen.	peptostreptococcus anaerobius vpi 4330-1 was exposed to atmospheric oxygen in a dilution bland (0.2% gelatin, salts, resazurin) solution. the organisms were rapidly killed when the solution contained cysteine. the organisms were effectively protected by catalase and horseradish peroxidase as well as by the metal ion-chelating agents 8-hydroxyquinoline and 2,2'-bipyridine. superoxide dismutase increased the rate of killing of the organisms, whereas singlet oxygen quenchers and scavengers of hydro ...	1979	453819
[epithelial permeability study of the bronchi in chronic bronchitis using an electron-dense tag].	an electron dense tracer, horseradish peroxidase (hrp), treated with dab-h2o2 for electron histochemical demonstration of peroxidase was used in an effort to find out whether or not the human bronchial epithelium was permeable to some macromolecules in chronic bronchitis. the biopsy specimens obtained from 5 patients at bronchoscopy and cultured in a medium with 2% hrp for 1-6 hours showed the reaction product to be distributed within intracellular spaces of the epithelium as well as in some ves ...	1979	454230
axon trajectories of renshaw cells in the lumbar spinal cord of the cat, as reconstructed after intracellular staining with horseradish peroxidase.		1979	455060
efferent projections of the parabigeminal nucleus in rats: a horseradish peroxidase (hrp) study.	the parabigeminal nucleus of the rat was made up of the dorsal, middle and ventral subgroups. when hrp was introduced into the superficial layer of the superior colliculus, labeled neurons were observed in the ipsilateral dorsal and ventral subgroups and in the contralateral middle subgroup. it was demonstrated that neurons of the dorsal and ventral subgroups sent fibers to the ipsilateral superior colliculus, and that those of the middle subgroup sent them to the anterior half of the contralate ...	1979	455073
cat phrenic nucleus architecture as revealed by horseradish peroxidase mapping.	cat phrenic motoneurons, labeled by intradiaphragmatic injection of horseradish peroxidase, formed a tight cluster in the most ventral portion of the ventral horn in lamina ix of the lower cervical cord. cell counts were symmetrically distributed for 17 to 21 mm along the longitudinal axis of the cord with a unimodal peak at the junction of segments c5 and c6. the phrenic nucleus was bilaterally organized on either side of the cord with anatomical symmetry and in no case was there evidence for t ...	1979	456448
the projection of the temporal retina in rats, studied by retrograde transport of horseradish peroxidase.	horseradish peroxidase (hrp) was injected unilaterally into the lateral geniculate nucleus or tectum, or both, in 26 hooded rats in order to mark the exact extent of the retina from which uncrossed optic axons arise. this region occupied about a quarter of the retina, in the temporal periphery, following thalamic injections, but a much smaller region following tectal injections. by comparing the proportions of hrp positive neurones in nasal and temporal retinae of both eyes it was shown that: (1 ...	1979	456453
morphological evidence for axonal sprouting of cerebellothalamic neurons in kittens after neonatal hemicerebellectomy.	changes in cerebellothalamic projections in kittens after neonatal hemicerebellectomy were studied by the retrograde and anterograde horseradish peroxidase (hrp)-tract-tracing methods. the number of cerebellar nuclear neurons labeled retrogradely with hrp injected into the ipsilateral va-vl complex of the thalamus was much more numerous in neonatally hemicerebellectomized kittens than in intact kittens. presumed terminals of ipsilateral cerebellothalamic fibers labeled anterogradely with hrp inj ...	1979	456456
permeability of retinal capillaries in rats with inherited retinal degeneration.	the permeability of retinal capillaries in rcs rats with inherited retinal degeneration was investigated with horseradish peroxidase used as a tracer. five-week-old rats showed typical degeneration of photoreceptor cells and accumulation of outer segment debris, but retinal capillaries were not permeable to peroxidase. at 10 weeks of age, capillaries in the inner retina appeared normal, but many in the outer retina were leaky. peroxidase activity in these latter vessels was demonstrable in the b ...	1979	457359
horseradish peroxidase as a label of injured cells.	the present study is concerned with artifacts likely to occur in a horseradish peroxidase exclusion test. incubation of murine peritoneal macrophages and lymphocytes with the peroxidase showed a close relationship between the number of living cells and the percentage of cells excluding the tracer. the penetration of the cytoplasm by horseradish peroxidase is attributed to an increase in the permeability of the cell membrane during the incubation (ranging from 10 to 120 min). it was not increased ...	1979	457441
morphological studies of stimulated adrenergic axon varicosities in the mouse vas deferens.	the postganglionic axons of sympathetic neurons innervating the mouse vas deferens were stimulated transmurally in vitro by passing square pulses between two platinum electrodes. the ultrastructural appearance of the adrenergic nerve terminals was compared to samples fixed immediately after 30 min of stimulation and in samples allowed to recover for 2 h before fixation. the contralateral vasa deferentia served as controls, and these were incubated in krebs solution for the same period as stimula ...	1979	457747
effect of concanavalin a dose, unbound concanavalin a, temperature, ca2+ and mg2+, and vinblastine on capping of concanavalin a receptors of human peripheral blood lymphocytes.	the capping of concanavalin a (con a) receptors induced by con a was studied using human peripheral blood lymphocytes. the effects of con a dose (5--100 micrograms/ml), pretreatment at 4 degrees c, unbound con a, extracellular ca2+ and mg2+ and vinblastine were evaluated using con a-horseradish peroxidase and electron microscopy. lymphocytes incubated with con a at 4 degrees c and fixed with glutaraldehyde exhibited con a-horseradish peroxidase around the entire cell periphery. after raising the ...	1979	457812
localization of vagal cardioinhibitory preganglionic neurons with rat brain stem.	in chloralose-urethane anesthetized spinal rats, electrical stimulation of systematically chosen points over the entire caudal brain stem area was carried out to explore the site(s) responsible for vagally mediated bradycardia. a dorsomedial locus including the nucleus dorsalis and the adjacent structures, the nucleus tractus solitarius, the nucleus commissuralis and the area postrema, and a ventrolateral locus around the nucleus ambiguus were found to elicit bradycardia with low threshold and h ...	1979	457932
latent lymphokines: isolation of guinea pig latent lymphocyte-derived chemotactic factor for monocytes. its activation by trypsin and a soluble factor from macrophages.	guinea pig lymphocytes when depleted of macrophages and stimulated by the t cell mitogen phytohemagglutinin produce a latent form of lymphocyte-derived chemotactic factor for monocytes (ldcf-m). latent ldcf-m is also produced when immune lymphocytes are stimulated in vitro with specific antigen, horseradish peroxidase. latent ldcf-m from both sources can be activated and converted to "classical" ldcf-m by trypsin and by a soluble factor obtained from sonicated macrophages. these observations sug ...	1979	458146
uptake and transport of horseradish peroxidase by lymphoepithelium of the bronchus-associated lymphoid tissue in normal and bacillus calmette-guérin-immunized and challenged rabbits.		1979	459427
[demonstration of the sources of several descending forebrain systems using the horseradish peroxidase retrograde axonal transport technic].	cells of cortical and diencephalic fibres descending to the mesencephalic and bulbar reticular formation and to the dorsal column nuclei were identified. according to findings neurons sending fibres to the mesencephalic reticular formation are located in orbitofrontal pole and in area 6 of the pericruciate cortex, mainly ipsilaterally. neurons which distribute fibres to the bulbar reticular formation are concentrated mainly in area 6 and 4 of the both hemispheres, while those distributing fibres ...	1979	460493
cortical afferents to the prefrontal cortex of the cat: a study with the horseradish peroxidase technique.	following horseradish peroxidase (hrp) injections into different areas within the prefrontal cortex (pfc) of the cat, labeled neurons were found in the cingulate and insular cortex. these results demonstrate that the cat's prefrontal cortex is reached directly from these cortical regions, and that the observed cortical projections are similar to those detected in the monkey's prefrontal cortex.	1979	460681
the precise origin of the tectospinal pathway in three common laboratory animals: a study using the horseradish peroxidase method.	the horseradish peroxidase tracing method has been used to study the cells of origin of the tectospinal projections in the opossum, the tree shrew, and the cat. the present data show that only those collicular neurons which occupy the deep (ventral to the stratum opticum) tectal laminae send axons to the cervical spinal cord. in particular, layer iv contains the greatest number of spinal projecting neurons. our results also reveal that while only the large sized collicular neurons project upon t ...	1979	460682
topographical representation in rabbit cerebellar flocculus for various afferent inputs from the brainstem investigated by means of retrograde axonal transport of horseradish peroxidase.	a small amount of horseradish peroxidase (hrp) was injected into several small areas of the flocculus and adjacent ventral paraflocculus of albino rabbits. labeled cells were surveyed through the inferior olive, vestibular nuclear complex, nucleus reticularis tegmenti pontis, pontine nucleus and abducens nucleus. distribution of labeled neurons in these structures varied depending on the location of injection sites, indicating the existence of a fine topographical organization for afferent input ...	1979	460699
enhancing the anterograde movement of hrp to label sparse neuronal projections.	by combining techniques designed to increase the amount of horseradish peroxidase (hrp) available for transport and to improve the sensitivity of the reaction for detecting hrp, we have produced an anterograde hrp technique suitable for labeling sparse efferent projections. the efficacy of this light, microscopic technique was demonstrated by lebeling the hippocampal commissural and crossed temporo-ammonic projections to the dentate gyrus of the rat.	1979	460700
the site of origin of cardiac preganglionic fibers of the vagus nerve: an hrp study in the cat.	after injection of horseradish peroxidase (hrp) into the right cardiac branches of the vagus nerve in the cat, the majority of hrp-labeled neurons were located ipsilaterally in the reticular formation ventrolateral to the nucleus ambiguus. additionally, hrp-labeled neurons were also observed within the nucleus ambiguus (am) and the dorsal motor nucleus of the vagus nerve (dm).	1979	460701
the comparative effectiveness of the "brown and blue reactions" for tracing neuronal processes of cells injected intracellularly with horseradish peroxidase.	the comparative advantages and disadvantages of diaminobenzidine (dab) and benzidine di--hcl (bdhc) procedures for tracing processes of cells injected intracellularly with horseradish peroxidase (hrp) have been studied. the dab reaction gives a good definition of membranes and appears more suitable for visualizing the fine structure of dendrites. the bdhc procedure, being more sensitive, induces crystal formation and heavily filled processes appear frosted. this last procedure is, however, much ...	1979	460707
projections to the ventral surface of the cat brainstem demonstrated by horseradish peroxidase.	topical application of horseradish peroxidase (hrp) to the ventral surface of the medulla oblongata revealed various cells with axons terminating near this region. fibers traced from the ventral surface originated in the nucleus of the tractus solitarius and the dorsal nucleus of the vagus, both near the dorsal surface of the medulla. projections from the cochlear nucleus were also observed. using short hrp contact time, fibers were shown which terminate at, or near, the ventral surface. the pos ...	1979	460710
early influx of horseradish peroxidase into axons of the hypoglossal nerve during wallerian degeneration.	horseradish peroxidase (hrp) was applied around mouse hypoglossal nerves which were damaged by a crush or a ligature. hrp was then visualized distal to the lesions by light- and electron microscopic histochemistry. at the injury the enzyme entered axons and could also be detected several millimetres down in the distal segment. by 24 h reaction product (r.p.) was either diffusely distributed in the axoplasm or present in various vesicular organelles. our results indicate that there is a rapid inf ...	1979	460736
the morphopathologic substrates of concussion?	neuronal inundation with i.v. infused horseradish peroxidase was studied following concussive brain injury by means of both light and electron microscopy. in animals sustaining mechanical brain injury of insufficient intensity as to elicit either microscopic intraparenchymal hemorrhage or other neuropathological change, yet of sufficient intensity as to provoke a physiological concussive response, vascular peroxidase exudation concomitant with neuronal peroxidase inundation occurred throughout t ...	1979	463502
the laminar and size distribution of commissural efferent neurons in the cat visual cortex.	the laminar and size distribution of commissural efferent neurons were studied in the cat visual cortex including area 17, 18, 19 and the lateral suprasylvian cortex (the lateral wall of the middle suprasylvian gyrus) by means of retrograde transport of horseradish peroxidase. single or multiple injections of the enzyme were made unilaterally along the medial part of the lateral gyrus (the adjoining parts of area 17 and 18) or along the lateral wall of the middle suprasylvian gyrus. in the contr ...	1979	464747
localization of lumbar epaxial motoneurons in the rat.	in the rat, lateral longissimus and the lumbar transverso-spinalis muscles are lumbar trunk muscles, and participate in the lordosis reflex (female sexual posturing). medial longissimus, the remaining major lumbar epaxial muscle, is a muscle of the proximal tail-tailbase. to allow an analysis of the motor control of lordosis, motoneurons for these muscles must be localized. horseradish peroxidase (hrp) was injected into lumbar epaxial muscles of female rats. following injection into medial longi ...	1979	466406
gaba-accumulating neurons in the nucleus raphe dorsalis and periaqueductal gray in the rat: a biochemical and radioautographic study.	the possibility of a gabaergic innervation of the nucleus raphe dorsalis (nrd) has been investigated by using the following approaches: (i) the identification of the principal neuronal groups afferent to the ndr by using horseradish peroxidase retrograde transport, (ii) the determination of glutamate decarboxylase activity (gad) in the nrd after lesioning these groups or their putative pathways, and (iii) the radioautographic identification of terminals axons and nerve cells accumulating intrave ...	1979	466412
spinal origin of preganglionic fibers projecting onto the superior cervical ganglion and inferior mesenteric ganglion of the guinea pig, as demonstrated by the horseradish peroxidase technique.		1979	466457
thalamic projections to the feline motor cortex studied with horseradish peroxidase.	cells in the thalamus projecting to the distal forelimb regions of the motor cortex, area 3a, and the sensory cortex were identified with horseradish peroxidase (hrp). these cortical areas were defined by mapping evoked potentials from superficial and deep radial nerve stimulation. following injections into the motor cortex, the labelled cells were distributed throughout a large region (greater than 2 mm wide) in the ventral portion of the ventral lateral nucleus (vl). the border area between vl ...	1979	466471
the identification of the cell bodies of vagal efferent fibres of pulmonary and cardiac origin in the cat using horseradish peroxidase [proceedings].		1979	469808
the central origin of efferent pathways in the carotid sinus nerve of the cat.	the application of horseradish peroxidase to the central cut end of the carotid sinus nerve of the cat produced retrograde labeling of neurons in the ipsilateral medulla in the region of the nucleus ambiguus at anterior-posterior coordinates -8 to -10.5. these data coupled with previous electrophysiological observations suggest that the nucleus ambiguus may be the origin of an efferent inhibitory pathway to the carotid body.	1979	472721
subfornical organ efferents to neural systems for control of body water.	the subfornical organ, a circumventricular structure of the central nervous system, has efferent neural projections to sites within the brain known to be involved in drinking behavior and secretion of antidiuretic hormone. by using anterograde tracing techniques, it is shown that the subfornical organ projects to the nucleus medians of the medial preoptic area, to the organum vasculosum of the lamina terminalis, and to the supraoptic nuclei bilaterally. its efferent connectivity is confirmed by ...	1979	472723
ultrastructure of the hepatic perisinusoidal cells in man and other mammalian species.	perisinusoidal (p.s.) cells occurring in the spaces of disse in the livers of normal cats, dogs, miniature pigs, albino rats, human adults and children were examined by electron microscopy. the ultrastructural details of the p.s. cells and their topographic relationships with hepatocytes, sinusoidal lining cells and reticulum fibers are described. species differences between p.s. cells were primarily a dissimilarity in lipid content: the main ultrastructural features were the same in all species ...	1979	475017
origin of descending afferents to the rostral part of dorsal cap of inferior olive which transfers contralateral optic activities to the flocculus. a horseradish peroxidase study.	neurons in the ventromedial tegmental area that project to the rostral part of the dorsal cap of the ipsilateral inferior olive were labeled by the technique of retrograde transport of horseradish peroxidase (hrp). following iontophoresis of hrp into the rostral part of the dorsal cap a cluster of labeled cells are found ipsilaterally in the area ventromedial to the red nucleus as well as in the area between the substantia nigra and the medial lemniscus. following hrp injections into the medial ...	1979	476490
a combined horseradish peroxidase and golgi study on the afferent connections of the ventrobasal complex of the thalamus in the cat.	afferent connections to the ventrobasal complex (vb) of the thalamus were studied by means of retrograde transport of horseradish peroxidase (hrp) and by the golgi-method. after hrp-injection into the vb, peroxidase-positive cells were observed contralaterally in the dorsal column nuclei (dcn), in the trigeminal nuclei and in the lateral cervical nucleus (lcn), and ipsilaterally in the somatosensoty i (si) and ii (sii) cortical areas. labeled cells of different shape and size were compared with ...	1979	476813
teratocarcinoma stem cells have a cell surface carbohydrate-binding component implicated in cell-cell adhesion.	teratocarcinoma stem cells maintained in the undifferentiated state express a carbohydrate-binding component that recognizes oligomannosyl residues. this cell surface molecule is detected by a rosetta assay in which the stem cells form rosettes with glutaraldehyde-fixed trypsinized rabbit erythrocytes. addition of simple sugars to the assay mixture has little effect, but rosette formation is inhibited by a series of mannose-rich glycoproteins (yeast invertase, yeast mannans and horseradish perox ...	1979	476826
antithyroid and antiperoxidase activity of tropolone and 3-hydroxy-4-pyrone.	tropolone (tr) and 3-hydroxy-4-pyrone were investigated for antithyroid activity following the finding that the 2-hydroxy-oxo pyridine, 3-hydroxy-4(1h)-pyridone (dhp, i), is goitrogenic. both compounds inhibited the thyroidal uptake of radioiodine in rats and resembled the thioamide drugs in inhibiting the organic binding of iodine by the thyroid gland rather than the trapping of iodide, but were weaker binding inhibitors than 6-methyl-2-thiouracil (metu). both compounds also inhibited the iodin ...	1979	476852
surgical procedure for exposure of the chorda tympani in dogs: a ventral approach.	an experimental neuroanatomic investigation of the innervation of the dog's tongue by the method of retrograde axonal transport of horseradish peroxidase required that chorda tympani neurectomy be performed in neonatal pups. surgical exposure and transection of the chorda tympani proximal to its union with the lingual branch of the mandibular nerve in the past has been accomplished by radical lateral approach which is suited only for acute experimentation. this report describes a ventral surgica ...	1979	477326
experimental study of spinal nerve repair after plexus brachialis injury in newborn rats: a horseradish peroxidase study.		1979	477790
fate of plasma membrane during endocytosis. ii. evidence for recycling (shuttle) of plasma membrane constituents.	cultured rat embryo fibroblasts were first allowed to store for 24 h fluorescein-labeled goat immunoglobulins directed against rabbit immunoglobulins (f anti-r igg), and were subsequently exposed for 24 h to [(3)h]acetylated rabbit immunoglobulins known to bind to the cell membrane either specifically (anti-plasma membrane igg: a anti-pm igg) or unspecifically (contol igg: ac igg). as a result of immunological interaction between the two antibodies (no effect was found if the cells had been prel ...	1979	479310
cytochemical characterization of secretory and cell surface glycoconjugates by light and electron microscopy.	lectin methods have increased the capacity for histochemical characterization and differentiation of glycoproteins and have demonstrated, for example, greater reactivity of gastrointestinal than of respiratory tract secretions with the periodate-concanavalin a-horseradish peroxidase method for localizing mannose-rich glycoprotein. application of a battery of ultrastructural cytochemical methods with specificity for the constituents characteristically present in the complex carbohydrates provides ...	1979	479563
studies on sensory neurons of the mouse with intracellular-recording and horseradish peroxidase-injection techniques.	1. dorsal root ganglion (drg) cells were dissected from the adult mouse with their peripheral nerves, and electrophysiological and morphological studies were performed. 2. the peripheral nerves were stimulateradish peroxidase (hrp) was injected into the cell body, and the size of stained cell body and axon, together with the state of myelination, were examined. 4. f-neurons, whose somatic spike is a tetrodotoxin (ttx)-sensitive na spike, had a large or medium-sized cell body with a myelinated ax ...	1979	479922
normal and benzo(a)pyrene-transformed fetal mouse brain cell. i. tumorigenicity and immunochemical detection of glial fibrillary acidic protein.	primary cultures of whole brain and cortex cells origination from 14-day-old a/jax or c3h mouse fetuses were treated with benzo(a)pyrene (b(a)p) for 24 h. after 7 to 8 passages a malignant transformation was observed in the chemically treated whole brain and cortex cultures. control cultures of cortex remained non-transplantable during the whole experiment (up to 14 passages) whereas in the control cultures originating from whole brain a spontaneous transformation appeared after 11 passages. wit ...	1979	484209
enzyme replacement via liposomes. variations in lipid compositions determine liposomal integrity in biological fluids.	liposomes survive exposure to biological fluids poorly, extruding trapped enzymes, drugs, or solutes upon interaction with serum or plasma constituents. we have quantified the disruptive effects of human serum on liposomes and have studied whether various modifications in their phospholipid composition might produce liposomes with an increased carrier potential for application in vivo. multilamellar liposomes (phosphatidycholine 70:dicetyl phosphate 20:cholesterol 10) were prepared with 3h-label ...	1979	486550
a horseradish peroxidase study of the projections from the latero-posterior nucleus to three lateral peristriate areas in the rat.		1979	487075
spinal projections from the lower brain stem in the cat as demonstrated by the horseradish peroxidase technique. i. origins of the reticulospinal tracts and their funicular trajectories.	using a retrograde tracer technique with horseradish peroxidase (hrp) attempts were made to determine the origins of reticulospinal tracts and their funicular trajectories. reticulospinal tracts originating from the mesencephalic reticular formation (rf) were composed of: (1) descending projections arising from the cluster of cells located just lateral to the periaqueductal gray that course in the anterior funiculus (af) and ventral part of the lateral funiculus (lf) with ipsilateral predominanc ...	1979	487101
horseradish peroxidase iontophoretic intracellular labelling of cultured purkinje cells.		1979	487104
topographical organization in medullary reticulospinal systems as demonstrated by the horseradish peroxidase technique.		1979	487117
tactile neurons of the superior colliculus of the cat: input and physiological properties.	neurons in the strata intermediale and profundum of the cat superior colliculus (sc) previously have been shown to be organized somatotopically and to respond to tactile stimuli. the stimulus properties required for neuronal activation suggest that these neurons are excited by inputs from selected classes of high-velocity, sensitive cutaneous mechanoreceptors, and that stimulus-response relationships indicate only a narrow range of information concerning stimulus magnitude. the properties of the ...	1979	487121
afferent projections to the self-stimulation regions of the dorsal pons, including the locus coeruleus, in the rat as demonstrated by the horseradish peroxidase technique.	afferent projections to the dorsal pons of the rat have been studied using the horseradish peroxidase (hrp) technique. hrp injections were made in each of the following regions: the vicinity of the locus coeruleus (lc); the periventricular gray, medial to the lc; the medial parabrachial region, lateral to the lc; the ventral cerebellum, dorsal to the lc; and the pontine reticular formation, ventral to the lc. because intracranial self-stimulation (icss) has been obtained in these regions, the af ...	1979	487203
influence of electrical stimulation of the substantia nigra on spontaneous activity of raphe neurons in the anesthetized rat.	recent studies of afferent connections of the anterior raphe using the horseradish peroxidase technique have demonstrated a major projection originating in the substantia nigra (sn). the present acute electrophysiological study examined the influence of stimulation of the afferent on the activity of individual neurons in the raphe of the posterior midbrain and anterior pons (n = 51), and of a control group of cells (n = 15) located 2 mm lateral to the raphe. the predominant effect of sn stimulat ...	1979	487208
are there specialized junctions in the pars maculata of the distal tubule?	in the present study the tight junctions at the macula densa were compared to those of the adjacent straight and convoluted segments of the distal tubule using freeze fracturing and thin sectioning techniques. only insignificant differences were found in the number of strands and the apico-basal depth of the tight junctions in the three distal tubular segments of rat, dog and tree shrew. in experiments with horseradish peroxidase on mice and tree shrews, the tracer did not penetrate the apical j ...	1979	487402
the parieto-rubro-olivary pathway in the cat.	stimulation of the parietal association cortex as well as the frontal motor cortex elicited clearly extracellular unitary activities or field potentials in the ipsilateral inferior olive in the cat. the parietal-induced responses came out generally at a longer and more variable latency than the frontal-induced ones. this suggested the existence of an indirect pathway from the parietal association cortex to the inferior olive. the recording sites for the parietal-induced responses were located no ...	1979	488210
mesencephalic and pontine afferent fiber system to the facial neucleus in the cat: a study using the horseradish peroxidase and silver impregnation techniques.		1979	488225
afferent projections to the ventral tegmental area of tsai and interfascicular nucleus: a horseradish peroxidase study in the rat.	using the retrograde transport of horseradish peroxidase (hrp), a study has been made of projections to the ventral tegmental area of tsai (vta) and related dopaminergic cell groups (a 10). in order to minimise the possibility of damage to fibres of passage, a technique was evolved for the microiontophoresis of hrp such that minimal current strengths and durations were applied. in addition to a sham injection, control injections were also made to the medial lemnisuc, red nucleus, deep tegmental ...	1979	489776
the decussation of the retinothalamic pathway in the cat, with a note on the major meridians of the cat's eye.	we have studied the naso-temporal division of the retinothalamic pathway of the cat by making large unilateral injections of horseradish peroxidase into the lateral geniculate nucleus. in confirmation of previous work, our retinal whole-mounts show a distinct vertical decussation line separating the contralaterally projecting nasal retina from the ipsilaterally projecting temporal retina. the ipsilateral decussation line is quite sharp, while the contralateral decussation is somewhat more diffus ...	1979	489781
the retinothalamic pathways in siamese cats.	by injecting one lateral geniculate nucleus with large amounts of horseradish peroxidase (hrp), we have determined the retinal distributions of contra- and ipsilaterally projecting retinothalamic ganglion cells in the siamese cat. in accord with the data of others, we observe that large numbers of temporal ganglion cells, which normally send axons ipsilaterally, instead misproject to the contralateral thalamus. however, in contrast to the expectations raised by previous work, we do not find the ...	1979	489782
a cerebello-pulvino-cortical and a retino-pulvino-cortical pathways in the cat as revealed by the use of the anterograde and retrograde transport of horseradish peroxidase.	a cerebello-pulvino-cortical and a retino-pulvino-cortical pathways were revealed in the cat by means of the horseradish peroxidase (hrp) method. the sites of termination of the cerebellofugal and retinofugal fibers in the pulvinar nucleus (pul) were visualized by the use of the anterograde transport of hrp. the cerebello-pulvinar fibers were found to arise mainly from the parvicellular region of the lateral cerebellar nucleus and to terminate contralaterally in a narrow area at the extreme dors ...	1979	489783
afferent connections to the amygdaloid complex of the rat and cat. i. projections from the thalamus.	by the use of the retrograde transport of horseradish peroxidase (hrp), the projections from the thalamus to the amygdala were investigated in the rat and cat, with main emphasis on the former species. hrp was injected stereotactically by microiontophoresis in the various amygdaloid nuclei. several control procedures including the use of different approaches for the introduction of the micropipette were undertaken to eliminate the possibility of misinterpretation due to uptake of the protein by ...	1979	489786
the origin of descending pathways in the dorsolateral funiculus of the spinal cord of the cat and rat: further studies on the anatomy of pain modulation.	there is considerable evidence that the dorsolateral funiculus (dlf) of the spinal cord contains descending pathways critical for both opiate and brainstem stimulation-produced analgesia. to obtain a comprehensive map of brainstem neurons projecting to the spinal cord via the dlf, large injections of horseradish peroxidase (hrp) were made into the lumbosacral spinal cord of cat and rat. these injections were made caudal to midthoracic lesions which spared only a single dlf or ventral quadrant (v ...	1979	489790
the longitudinal zonal pattern in the paramedian lobule of the cat's cerebellum: an analysis based on a correlation of recent hrp data with results of studies with other methods.	in a preceding study of the distribution of retrogradely labeled cells in the inferior olive of the cat after microinjections of horseradish peroxidase (hrp) in the contralateral paramedian lobule (brodal and walberg, '77b), three longitudinal zones were distinguished. the zones were assumed to correspond to voogd's zones c1, c2 and d, receiving their afferents from parts of the dorsal accessory olive, of the medial accessory olive and of the dorsal lamella of the principal olive, respectively. ...	1979	489793
origin, course and terminations of the rubrospinal tract in the pigeon (columba livia).	the red nucleus and its spinal projections in the pigeon (columba livia) have been studied using both normal and experimental material. the cytoarchitecture of the nucleus is described on the basis of nissl-stained sections and reveals an organization generally similar to that of mammals. the large neurons (40-50 mum) tend to be located dorsomedially and ventrolaterally at more caudal nuclear levels, while the small- and medium-sized neurons (15-35 mum) predominate at rostral levels. however, ne ...	1979	489796
translocation of dimeric iga through neoplastic colon cells in vitro.	we studied the translocation of dimeric iga across epithelium, using neoplastic human colon cells in culture as a source of epithelial cells, and immunoelectronmicroscopy with peroxidase-labeled antigens and antibodies. the cells had some of the ultrastructural characteristics of normal, mature epithelial cells, i.e., polarity, desmosomal junctions, and secretory component on their basal and lateral plasma membranes. horseradish peroxidase-labeled dimeric iga, exposed to the cells at 0 degrees c ...	1979	489985
intestinal absorption of immune complexes by neonatal rats: a route of antigen transfer from mother to young.	horseradish peroxidase (hrp) in the presence of specific immunoglobulin g antibody to hrp is selectively absorbed from the gut lumen and transferred by intestinal epithelial cells to the lamina propria in newborn rats. the hrp is not transferred in detectable amounts in the absence of the antibody. transport of maternally derived antigen via antigen-antibody complexes may have important influences on the developing immune system in young mammals.	1979	493961
thalamocortical organization of the auditory system in the cat studied by retrograde axonal transport of horseradish peroxidase.		1979	495257
[cortico-cortical connections between the auditory fields and the sensomotor area of the cortex].	in 18 cats by means of two methods--anterograde degeneration and retrograde transport of exogenic horseradish peroxidase--cortico-cortical connections of the auditory fields to the cortical sensomotor area have been studied. these connections have been stated to terminate in layers v-iii of certain parts of the sensomotor area corresponding to the projections of the foreleg and the head. initial neurons of the connections studied are pyramidal cells in layers iii and ii. they are situated in ros ...	1979	496667