Publications

mental health and psychiatric nursing and primary health care: issues and prospects.		1986	261023
[an unusual case (retrobulbar neuritis with retromaxillary abscess originating from 27)].		1986	268708
[planned and active parenthood--the first stage of labor].		1986	260987
[results of triennial screening of a mountain community school population in ussl n. 42].	the paper reports data regarding dental caries in deciduous and permanent teeth in a sample population of 1015 pupils aged between 5 and 15, resident in the mountain community of the chisone and germanasca valleys. the dmf index was significantly high, especially if one takes into account that the partial findings referring to '89 are virtually unchanged in comparison to '87.	1986	2129585
law - three recent cases.		1986	238892
[prognostic indices of acute lymphoblastic leukemia in the child. proposal for a prognostic classification and case contribution].		1985	271763
the isolation of minicells.	the term minicell was introduced by adler et al. (1) for the small spherical cells produced by abnormal cell division at polar ends of certain escherichia coli cells. the phenomenon itself however, had already been described as early as 1930 (2) for a strain of vibrio cholera. these minicells do not contain any chromosomal dna, but they do inherit in most cases the plasmid dna present in the parental strain. since plasmid-containing minicells are able to synthesize dna, rna, protein, and other c ...	1985	21374210
[neonatal heart rate variability].		1985	261135
when the pressure rises.		1985	261252
the ada conference on increasing access to dental care. a report.		1985	268525
[genetic analysis of mutations affecting the synthesis of somatic o-antigen of vibrio cholerae].	the mutations oagm and oagr affecting the synthesis of somatic o-antigen have been localized on the chromosome of vibrio cholerae el jor and classic biotypes by conjugational crosses between different donor and recipient strains. the mutations are localized in the vicinity of the arg marker in both classic and el tor biotypes of vibrio cholerae.	1985	2432416
[determination of antibodies to streptococcus group a polysaccharide in human sera by an immunoenzyme method].	use was made of the elisa to develop a highly sensitive quantitative method for detection of antibodies against streptococcus group a polysaccharide (polysaccharide a) in human sera. the main advantage is that one can use only one optimal dilution of the sera together with the reference serum. sera of 53 healthy volunteers and 77 patients with a history of streptococcus group a infections were screened for the presence of polysaccharide a antibodies. highly reproducible results were obtained in ...	1985	2578835
rationale behind the development of low molecular weight heparin derivatives.	there is now considerable evidence that the antithrombotic and the hemorrhagic effects of heparin can be dissociated by using low molecular weight heparins and by using heparin with low affinity to at iii. the findings with low-affinity heparin suggest that hemorrhage is contributed to by heparin properties that are independent of at iii binding and thus of their major anticoagulant effect. the current evidence supports the suggestion that the hemorrhagic effect of heparin is contributed to by a ...	1985	2579436
cloning and characterization of the hemolysin determinants from vibrio cholerae rv79(hly+), rv79(hly-), and 569b.	the hly region from the chromosome of vibrio cholerae el tor strain rv79(hly-) and the nonhemolytic classical strain 569b were cloned into plasmid vector pbr322. escherichia coli k-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32p-labeled hly-specific dna probe. restriction endonuclease sau3ai digestions of the cloned hly loci of two independently obtained rv79(hly+) convertants, when compared with the digests of cloned rv79(hly-) loci, reveale ...	1985	2579940
intestinal infection with mycobacterium avium in acquired immune deficiency syndrome (aids). histological and clinical comparison with whipple's disease.	at endoscopy, a 30-year-old man with acquired immune deficiency syndrome (aids), kaposi's sarcoma, diarrhea, and unexplained malabsorption showed erythematous macular duodenal lesions consistent with whipple's disease by histology and electron microscopy. symptoms did not respond to tetracycline. subsequent cultures revealed systemic mycobacterium avium (m. avium) infection. tissue from this patient, from patients with whipple's disease and from a macaque with m. avium were compared. all contain ...	1985	2580679
leukocyte tubuloreticular inclusions in reye's syndrome.	tubuloreticular inclusions (tri) have been observed in the rough endoplasmic reticulum of blood lymphocytes and monocytes in two cases of reye's syndrome initiated by influenza infections. tubuloreticular inclusions are seen in these mononuclear leukocytes during the acute phase of illness, but not during convalescence. since tri have been demonstrated in peripheral mononuclear leukocytes in patients with acquired immunodeficiency syndrome, systemic lupus erythematosus, and certain viral infecti ...	1985	2581524
nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve section.	the sciatic nerve was sectioned unilaterally in rats and nerve growth factor (ngf) applied locally to the nerve stump for the following 10-14 days using an indwelling osmotic pump. the aim of the experiment was to test whether ngf had any effect on the previously reported neurophysiological and neurochemical events that occur central to a peripheral nerve lesion. the method of application allowed the sciatic nerve on the other side to be used as a control. primary afferent depolarization fell, a ...	1985	2581648
transport of hydrophobic ions in erythrocyte membrane: i. zero membrane potential properties.	the permeability and partition coefficients of tetraphenylarsonium (tpa) and several other organic cations were studied in the human erythrocyte using an ion-selective electrode. the permeability constant for the different cations could be explained quite well by differences in oil/water partition coefficients. no evidence for facilitated transport could be found. binding of the organic ions occurred to both the cell membrane and to intracellular contents. partitioning to the membrane remained r ...	1985	2582132
fluorescent staining of sialidases in polyacrylamide gel electrophoresis and ultrathin-layer isoelectric focusing.	polyacrylamide gels were stained with the sialidase substrate 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid showing the activity of vibrio cholerae and clostridium sordellii sialidases in the gels after electrophoresis. with this fluorogenic method minimum sialidase activities of 5 microu could be determined. the sensitivity of this staining is about 10,000-fold higher compared to protein-staining with coomassie brilliant blue. for the visualization of other proteins than sialidases ...	1985	2409836
use of non-carbonated soft drinks to provide safe drinking water.	non-carbonated, low-calorie soft drink concentrates (cordials), when diluted according to manufacturers' instructions, had significant antibacterial effects in vitro. bacteria affected include vibrio cholerae, aeromonas hydrophila, shigella sonnei, salmonella typhimurium and escherichia coli. with vibrios, bacterial counts were reduced from 10(6)/ml to undetectable numbers in less than 10 min. escherichia coli in an initial concentration of 10(6)/ml became undetectable after incubation for 1 h w ...	1985	2409896
catalase enhances damage to dna by bleomycin-iron(ii): the role of hydroxyl radicals.	bleomycin degrades dna under aerobic conditions when a ferrous salt is added. this reaction is enhanced by catalase and certain hydroxyl radical scavengers but inhibited by the addition of hydrogen peroxide. a ferricbleomycin complex is, however, stimulated by addition of hydrogen peroxide. these findings suggest that catalase removes hydrogen peroxide and in so doing prevents loss of ferrous ions and formation of hydroxyl radicals (oh.) by a fenton-type reaction. it further suggests that oh. ra ...	1985	2409975
rapid detection of vibrio cholerae o:1 by motility inhibition and immunofluorescence with monoclonal antibodies.	monoclonal antibodies against the group and type specific antigens of vibrio cholerae o:1 lipopolysaccharide were used for the rapid detection of vibrio cholerae strains by motility inhibition and immunofluorescence. motility inhibition of live vibrio cholerae o:1 was obtained with group specific monoclonal antibodies. monoclonal antibodies against the type specific antigens b (ogawa) and c (inaba) inhibited motility of strains of homologous serotypes only. indirect immunofluorescence of heatfix ...	1985	2410265
immunological characterization of vibrio cholerae o:1 lipopolysaccharide, o-side chain, and core with monoclonal antibodies.	lipopolysaccharide (lps) was extracted from vibrio cholerae o:1 strains of the serotypes ogawa, inaba, and hikojima and delipidated by mild-acid hydrolysis. two polysaccharide fragments with the molecular weights of approximately 9,000 and 900, respectively, were isolated by gel permeation chromatography. the lps preparations and the polysaccharide fragments were studied in enzyme-linked immunosorbent assay inhibition, rocket immunoelectrophoresis, and sodium dodecyl sulfate-polyacrylamide gel e ...	1985	2410362
membrane-bound 5'-nucleotidase in marine luminous bacteria: biochemical and immunological properties.	a novel 5'-nucleotidase previously described in halophilic vibrio costicola was detected in marine vibrio and photobacterium strains. the enzyme of marine bacteria was similar in its properties to the 5'-nucleotidase of vibrio costicola; it was outwardly oriented in the cytoplasmic membrane and dephosphorylated nucleoside 5'-tri-, di-, and mono-phosphates to respective nucleosides before uptake. the enzyme in marine strains was immunologically cross-reactive with the antibody raised against the ...	1985	2411368
[basic factors of the pathogenicity of vibrio cholerae].		1985	2412368
a study on the pharmacokinetics in mouse of adenine-9-beta-d-arabinofuranoside 5-monophosphate conjugated with lactosaminated albumin.	in plasma of mice injected with adenine-9-beta-d-arabinofuranoside monophosphate (ara-amp) coupled to human lactosaminated serum albumin (l-hsa) some of the ara-amp molecules are enzymatically released, whereas others remain linked to l-hsa. evidence has been obtained that ara-amp is not deaminated when it is conjugated to l-hsa, in contrast to the free drug which is rapidly metabolized to its hypoxanthine derivative.	1985	2412878
a simple method for counting nuclei in the preimplantation mouse embryo.	an easy and rapid method of counting the number of cells in the preimplantation mouse embryo is described. the procedure increases the speed with which large numbers of embryos can be processed using a simple squash technique. cell numbers are determined by exposing the embryos to the fluorescent dna-binding dye, hoechst 33258, removing the zona pellucida and simply squashing the embryo and counting the number of fluorescent nuclei. an increase in fluorescent intensity and maintenance of nuclear ...	1985	2412884
expression of epidermal keratins and filaggrin during human fetal skin development.	the major structural proteins of epithelia, the keratins, and the keratin filament-associated protein, filaggrin, were analyzed in more than 50 samples of human embryonic and fetal skin by one-dimensional sds page and immunoblotting with monoclonal and polyclonal antibodies. companion samples were examined by immunohistochemistry and electron microscopy. based on structural characteristics of the epidermis, four periods of human epidermal development were identified. the first is the embryonic p ...	1985	2413039
genetics of insulin-specific helper and suppressor t cells in nonresponder mice.	the role of insulin-specific helper and suppressor t cells in the h-2-linked genetic control of antibody responses to heterologous insulins was examined in vitro. these data demonstrate that pork insulin stimulates both primed helper t cells and dominant suppressor t cells in all nonresponder strains tested. thus, the nonresponder phenotype is attributed to the activation of specific suppressor t cells rather than to an absence of helper t cell activity. examination of the antigenic cross-reacti ...	1985	2413109
characterization of an unique rna initiated immediately upstream from human alpha 1 globin gene in vivo and in vitro: polymerase ii-dependence, tissue specificity, and subcellular location.	we have identified an abundant transcript initiated upstream from the canonical cap site of human alpha 1 globin gene in bone marrow cells and in cos-7 cells transfected with an alpha 1 globin gene-containing plasmid. similar to the major alpha 1 globin transcript, this upstream rna is present almost exclusively in the cytoplasm of the transfected cos-7 cells. it is also synthesized efficiently in vitro by rna polymerase ii in the nuclear extracts prepared from a hela cell line and an erythroleu ...	1985	2413422
protective characteristics of the antigen common to pseudomonas and vibrio and significance of bacterial endotoxins in protection against selected bacterial infections.	the protective effect of different vaccines against the killing activity of pseudomonas aeruginosa and vibrio parahaemolyticus for mice was studied. cross experiments confirmed the high protective effect of the pseudomonas common antigen (original endotoxin protein) contained in pseudomonas vaccine, which, together with other soluble components, substantial afforded protection of mice against vibrios and thus confirmed the antigenic relationship between pseudomonas and vibrio. satisfactory resul ...	1985	2413656
syngeneic monoclonal antibody against melanoma antigen with interspecies cross-reactivity recognizes gm3, a prominent ganglioside of b16 melanoma.	it has previously been reported that a mouse (c57bl/6) monoclonal antibody, m2590, was established against syngeneic melanoma b16 cells, which was shown to react only with melanoma cells from various species but not with other tumor cells or normal tissues (taniguchi, m., and wakabayashi, s. (1984) gann 75, 418-426). in the present study, the specificity of m2590 antibody was shown to be directed to a saccharide arrangement (neuac alpha 2-3gal beta 1-4glc (or -glcnac)) of gangliosides by three d ...	1985	2414279
transcriptional and post-transcriptional regulation of l-type pyruvate kinase in diabetic rat liver by insulin and dietary fructose.	regulation of the expression of the hepatic l-type pyruvate kinase gene by insulin and dietary fructose was studied in diabetic rats. insulin increased the levels of putative nuclear rna precursor species of this enzyme in parallel with that of total cellular pyruvate kinase l mrna. these changes occurred more slowly than those induced by dietary fructose. insulin caused a 3-fold increase in transcription of the pyruvate kinase l gene after 6 h and a 6-fold increase after 16 h. the increase caus ...	1985	2414297
[vital staining of the conjunctiva for prevention of epithelial implantation cysts].	selective vital staining with 1% toluidine blue makes short-term intraoperative differentiation of the conjunctiva from tenon's capsule possible. it is used with advantage in operations where the anatomical situation is unclear to avoid displacement of conjunctival epithelium into deeper tissues and subsequent growth of cysts. examples of its application are reoperations with excessive scars after squint or detachment surgery, wound dehiscence after enucleations, lacerated wounds after accidents ...	1985	2415747
differences in carbohydrate specificities and complement-activating capacity of guinea pig and human antibodies to neuraminidase-treated autologous erythrocytes.	guinea pig erythrocytes desialated by treatment with neuraminidase from vibrio cholerae were lyzed in autologous serum through a natural-antibody-dependent activation of the classical complement pathway. lysis was inhibited when a mannose, glucose, galactose or n-acetyl-glucosamine was added to the incubation mixture. methyl-alpha- or -beta-d-galactopyranosides were poorly effective and n-acetyl-d-galactosamine was not effective at all. inhibition of lysis by the carbohydrates was due neither to ...	1985	2415814
isolation and characterization of influenza c virus inhibitor in rat serum.	two hemagglutination inhibitors for influenza c virus were isolated from pooled sera of normal rats by sequential chromatography on blue sepharose cl 6b, ultrogel aca 22, and deae-cellulose. the two inhibitors were identified as alpha 1-macroglobulin and murinoglobulin by comparison with the authentic samples. these inhibitors abolished the hemagglutination by influenza c virus strains but did not affect the hemagglutination by influenza a and b virus strains. hemagglutination inhibition activit ...	1985	2416143
electrophoretic resolution of microheterogeneity in vibrio cholerae lipopolysaccharide.	lipopolysaccharide (lps) from vibrio cholerae has been analysed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. under normal conditions of electrophoresis which resolve escherichia coli lps, v. cholerae lps shows two diffuse and unresolved bands. however, on long gels at low concentration it can be resolved into two major band types. there are at least 10 slow moving, discrete bands of regular periodicity and three fast moving bands. comparison with lps from e. coli indicates that ...	1985	2417638
effect of carrier on the immunogenic capacity of synthetic cholera vaccine.	in the design of the synthetic antigens and synthetic vaccines, primary consideration should be given to the choice of the carrier. since small peptides which are being used as the relevant antigenic determinants are likely to be poor immunogens as such, the augmentation of their immunogenic capacity by the carrier or any other means is crucial for the induction of immunity. in the present study, we explored several approaches for the enhancement of the immune response towards synthetic peptides ...	1985	2421153
bone marrow structure and its possible significance for hematopoietic cell renewal.	the authors review the progress made during the last quarter of a century in the fields of hematopoietic cellular proliferation and differentiation in relation to the bone marrow structure and the microenvironment provided by the marrow stroma in which unlimited self-renewal occurs. the marrow is conceived of as an organ in which the stroma originates from local mesenchymal elements which form a vascularized and innervated matrix, seeded later by blood-borne stem cells. transplantation studies u ...	1985	2869731
the adjuvant effect of pertussis endotoxin protein in modulating the immune response to cholera toxoid in mice.	endotoxin protein represents a group of immunobiologically active p polypeptides which are associated with the lipopolysaccharide endotoxin in the outer membrane of gram-negative bacteria. to study the adjuvant effect of endotoxin protein, cf-1 mice were immunized intraperitoneally with graded doses of glutaraldehyde-inactivated cholera toxoid with and without endotoxin protein prepared from bordetella pertussis, salmonella typhi or vibrio cholerae. immune responsiveness was assessed by measurin ...	1985	2872108
immunomodulation by bordetella pertussis: antiviral effects.	treatment of mice by intraperitoneal inoculation of pertussis vaccine or lipopolysaccharide extracted from b. pertussis will effect resistance to rabies virus, encephalomyocarditis virus, semliki forest virus, and herpes simplex virus. our previous observations indicated that treatment of c3h/hen (+/nu) and bdf1 mice with pertussis vaccine injected i.p. five days prior to a mouse adenovirus lethal dose i.p. challenge elicited resistance to clinical disease and death. susceptibility returned to a ...	1985	2872109
[comparisons of vasoconstrictions induced by alpha agonists either in the luminal perfusate or in the adventitial superfusate].		1985	2869558
[effect of interferon on the absorption of staphylococci by the macrophages of mouse peritoneal exudate].		1985	2477669
polymicrobial aetiology of travellers' diarrhoea.	of 35 us peace corps volunteers in thailand, 20 (57%) had a total of 30 episodes of diarrhoea during their first 6 weeks in the country. enteric pathogens were associated with 90% of the episodes. a single pathogen was identified in 17 (57%) episodes, 2-4 pathogens were identified in 10 (33%) episodes, and there were 15 symptomless infections. enterotoxigenic escherichia coli (etec) was identified in 37% of these episodes, and various salmonella serotypes were isolated in 33%. infections with 9 ...	1985	2857430
preparation and properties of vibrio cholerae antifimbrial antibody.	the presence of fimbriae on the vibrio cholerae strains used was assessed by pellicle formation, haemagglutination activity and electron microscopy. fimbrial suspensions were prepared by shearing them off the organisms, then separating them from other components by absorbing them on to rabbit red blood cells. rabbits were then immunized with the fimbrial-red cell suspensions and the antibodies evoked were titrated by haemagglutination inhibition, agglutination, vibriocidal and immobilization tec ...	1985	2858464
the antibacterial effect of selected neuroleptics on vibrio cholerae.	the present investigation has been undertaken to illustrate the anti-bacterial effect of chlorpromazine, clopenthixol and levomepromazine-maleate. it has been shown that clopenthixol has the strongest antibacterial effect. it is known that chlorpromazine also has an antisecretory activity. the fact that clopenthixol exists in cis(z)- and trans(e)-isomerical compounds, and that these compounds both have an antibacterial effect, but only the cis-compound has an antisecretory and neuroleptic effect ...	1985	2858954
effects of a high-sucrose diet on the development of enzyme-altered foci in chemical hepatocarcinogenesis in rats.	dietary factors can modify metabolic events involved in the initiation, promotion, or progression of tumors. to determine whether a high-sucrose diet has any effect on the development of enzyme-altered foci during the promotion step of chemical hepatocarcinogenesis in rats, 1-day-old sprague-dawley rats were given a single i.p. dose of diethylnitrosamine; controls received an equivalent i.p. volume of 0.9% nacl solution. at 21 days of age, the rats were weaned, segregated by sex, separated in gr ...	1985	2859110
effects of a high-sucrose diet on the development of enzyme-altered foci in chemical hepatocarcinogenesis in rats.	dietary factors can modify metabolic events involved in the initiation, promotion, or progression of tumors. to determine whether a high-sucrose diet has any effect on the development of enzyme-altered foci during the promotion step of chemical hepatocarcinogenesis in rats, 1-day-old sprague-dawley rats were given a single i.p. dose of diethylnitrosamine; controls received an equivalent i.p. volume of 0.9% nacl solution. at 21 days of age, the rats were weaned, segregated by sex, separated in gr ...	1985	2859110
striatal synaptosomal dopamine synthesis: evidence against direct regulation by an autoreceptor mechanism.	regulation of the rate-limiting step in dopamine (da) synthesis was estimated in striatal synaptosomes by measuring the rate of hydroxylation of l-4-[3h]phenylalanine, a substrate of tyrosine hydroxylase (th). da inhibited hydroxylation with an ic50 of 0.2 microm. the concentration-response curve of da-induced inhibition was not affected by the presence of 1 microm chlorpromazine, a phenothiazine da antagonist. sulpiride and haloperidol, da antagonists of the benzamide and butyrophenone classes ...	1985	2859213
immunochemical recognition of oxytocin by antiserum to l-prolyl-leucyl-glycine amide.	antisera were raised in rabbits against pro-leu-gly-nh2 (plg)-bovine thyroglobulin conjugates. specificity of the antisera towards oxytocin was evaluated by a radioimmunoassay as well as by immunohistochemical procedures on hypothalamic tissue and hormone bound to sepharose beads. it was concluded that immunization with plg can raise antisera that are specific to oxytocin.	1985	2859563
prolactin and its involvement in fluid regulation in the bovine.	plasma prolactin (prl) concentrations, osmolality, water consumption, feed intake, urine excretion, and fecal water output were determined in twelve steers of 3 breeds exposed to 5 feed and water regimes. breed differences were found in water intake and plasma prl concentrations when feed and water were ad lib., however, during any of the other 4 treatments, responses were similar between breeds. during dehydration and feed restriction, water intake, urine, fecal water, and plasma prolactin decr ...	1985	2859961
induction of vibrio cholerae specific biliary antibodies after oral immunisation with a cholera cell-wall fraction.		1985	2860476
effect of phenobarbital on the gamma-glutamyltranspeptidase activity and the remodeling of nodules induced by the initiation-selection model.	the effect of subsequent administration of phenobarbital on the gamma-glutamyltranspeptidase (ggt) activity and on the remodeling of nodules induced by the solt-farber procedure was examined in rats. ggt-nodules were initiated by diethylnitrosamine (dena) followed by selection with 2-acetylaminofluorene (2aaf) in the diet and a partial hepatectomy (ph). phenobarbital (500 ppm in the drinking water), administered to rats that were previously treated according to the solt-farber procedure, (1) inc ...	1985	2860967
rapid diagnosis of campylobacter-associated gastritis.		1985	2861379
photolabeling of mitochondrial f1-atpase by an azido derivative of the oligomycin-sensitivity conferring protein.	an azido derivative of the oligomycin sensitivity conferring protein (oscp) was prepared by alkylation with the bifunctional reagent p-azido phenacyl bromide. azido-oscp was fully biologically active in the dark. upon photoirradiation of a mixture of beef heart mitochondrial f1-atpase and azido-oscp, the resulting covalent photoproducts were separated by polyacrylamide gel electrophoresis in the presence of na dodecyl sulfate and characterized by an immunochemical procedure. oscp was found to re ...	1985	2861814
neuraminidase activity is not the cause of influenza virus-induced neutrophil dysfunction.	influenza viruses have been shown to decrease the ability of polymorphonuclear leukocytes (pmn) to respond to a variety of stimuli. this study was done to determine if viral neuraminidase was responsible for decreased pmn function. treatment of human pmn with purified neuraminidases from influenza virus, vibrio cholerae, or clostridium perfringens did not significantly affect the ability of human pmn to respond to stimulation. occasional virus preparations that lacked the ability to depress pmn ...	1985	2862160
comparative evaluation of three identification systems for anaerobes.	the accuracy of two new 4-h identification systems for anaerobes, the an-ident (analytab products, plainview, n.y.) and the rapid ana (innovative diagnostic systems, inc., atlanta, ga.) was compared with that of the api 20a system (analytab products). a total of 132 clinical isolates were tested in each of the three systems. the overall accuracies at the genus and species level for the three systems were: api 20a, 68.9 and 56.8%, respectively; an-ident, 90.2 and 73.5%; and rapid ana, 93.9 and 81 ...	1985	2862161
non-o1 vibrio cholerae produces two newly identified toxins related to vibrio parahaemolyticus haemolysin and escherichia coli heat-stable enterotoxin.		1985	2862364
[pathogenesis, clinical aspects and diagnosis of the most important enteric bacterial diseases in animals].	there are adhesive and invasive species among enteropathogenic bacteria for animals. most frequently isolated adhesive bacteria are enterotoxigenic e. coli (etec). salmonella, t. hyodysenteriae, m. paratuberculosis, y. enterocolitica, cl. perfringens and c. jejuni bacteria represent the most important invasive germs. the main clinical finding connected with bacterial enteritis is diarrhoea resp. dysentery. evaluation of feces consistency, time of appearance and accompanying symptoms (e.g. fever, ...	1985	2862716
effect of vector on infectivity of trypanosoma cruzi.	a comparative study was carried out on the interaction between triatoma infestans and bloodstream forms (bsf) of trypanosoma cruzi isolates ra and up, both lethal for mouse, and ca-i nonlethal for this host. parasite duplication was readily detected in triatomes fed with ca-i, metacyclic (mtc) differentiation reaching a maximum at an optimum ingestion level of 250 bsf/insect. progress and differentiation proved much lower for ra, but reached intermediate values for the up isolate. assays for inf ...	1985	2862777
effect of vector on infectivity of trypanosoma cruzi.	a comparative study was carried out on the interaction between triatoma infestans and bloodstream forms (bsf) of trypanosoma cruzi isolates ra and up, both lethal for mouse, and ca-i nonlethal for this host. parasite duplication was readily detected in triatomes fed with ca-i, metacyclic (mtc) differentiation reaching a maximum at an optimum ingestion level of 250 bsf/insect. progress and differentiation proved much lower for ra, but reached intermediate values for the up isolate. assays for inf ...	1985	2862777
protein phosphatases active on acetyl-coa carboxylase phosphorylated by casein kinase i, casein kinase ii and the camp-dependent protein kinase.	the protein phosphatases in rat liver cytosol, active on rat liver acetyl-coa carboxylase (acc) phosphorylated by casein kinase i, casein kinase ii and the camp-dependent protein kinase, have been partially purified by anion-exchange and gel filtration chromatography. the major phosphatase activities against all three substrates copurify through fractionation and appear to be identical to protein phosphatases 2a1 and 2a2. no unique protein phosphatase active on 32p-acc phosphorylated by the case ...	1985	2862868
protein phosphatases active on acetyl-coa carboxylase phosphorylated by casein kinase i, casein kinase ii and the camp-dependent protein kinase.	the protein phosphatases in rat liver cytosol, active on rat liver acetyl-coa carboxylase (acc) phosphorylated by casein kinase i, casein kinase ii and the camp-dependent protein kinase, have been partially purified by anion-exchange and gel filtration chromatography. the major phosphatase activities against all three substrates copurify through fractionation and appear to be identical to protein phosphatases 2a1 and 2a2. no unique protein phosphatase active on 32p-acc phosphorylated by the case ...	1985	2862868
lymphatic transport of sodium cefoxitin in the presence of sodium 5-methoxysalicylate after injection into rat rectal connective tissue, femoral muscle and femoral vein.	lymphatic uptake of sodium cefoxitin after injection into rectal connective tissue was greater than after injection into the femoral muscle of rats. coadministration with sodium 5-methoxysalicylate enhanced lymphatic drug uptake at both sites. this enhancement may be an indirect result of 5-methoxysalicylate's suppression of vascular permeation of the cefoxitin. an adjuvant-induced increase in lymphatic fluid flow may also be partially involved in the enhancement of cefoxitin lymphatic transport ...	1985	2863362
6,7-dihydro-5-[[(cis-2-hydroxy-trans-3-phenoxycyclopentyl)amino] methyl]-2-methylbenzo[b]thiophen-4(5h)-one: a novel alpha 1-adrenergic receptor antagonist and renal vasodilator.		1985	2863377
importance of highly sensitive serological tests for cytomegalovirus in bone marrow transplant patients.		1985	2863463
prospective study of acquisition of cryptosporidium, giardia lamblia, and gastrointestinal illness.		1985	2863502
detection of infectious htlv-iii/lav virus in cell-free plasma from aids patients.		1985	2863528
lav/htlv-iii antibodies in connective tissue diseases.		1985	2863569
the synaptic distribution of the retinal input in the superficial layers of the guinea-pig superior colliculus.	an evoked potential consisting of four postsynaptic components was recorded in the guinea-pig superior colliculus following electrical stimulation of the contralateral optic nerve. this potential was generated in response to the activation of four populations of optic nerve fibres with different conduction velocities. current source-density analysis revealed that the two slower conducting fibre populations synapse in the upper third of the stratum griseum superficiale on dendrites whose cell bod ...	1985	2863823
[the saprophytic phase in the ecology of the causative agents of infectious diseases].		1985	2863907
[changes in acetylcholine contractions induced by carnitine in coronary vessels isolated "in vitro"].	as suggested by literature about the carnitine's choline-mimetic effects, it has been studied the influence of this substance on the response to ach of the isolated coronary arteries. it has been seen that ach has often induced the contraction of the preparation that was preceded or abolished by atropine or prifinium bromide, or reduced by fendiline and verapamil. it resulted also that carnitine has always increased the entity of the contraction of the preparation treated with ach and that the r ...	1985	2863965
neurohumoral vasoconstrictor systems in heart failure.	by means of specific inhibitors of the renin-angiotensin system (captopril) and of the sympathetic nervous activity (prazosin) in dogs with congestive heart failure, and by using a specific antagonist of the pressor activity of arginine vasopressin in rats with heart failure, we studied the influence of these pressor hormone systems on peripheral vascular resistance and cardiac function. all three humoral vasoconstrictor systems were stimulated in heart failure. the experiments in dogs showed th ...	1985	2864248
[synthesis and properties of an affinity adsorbent for purifying neuraminidases of varying origins].	neuraminidases (na) from clostridium perfringens, noncholera vibrios and influenza virus were purified by affinity chromatography on sepharose coupled to para-aminophenyl oxamic acid. adsorption was carried out at ph 5.5. the effect of elution conditions on purification of na was studied. the use of the ph gradient enhances 10-fold the purification degree as compared with the ph shift-elution process, 90% of the activity being eluted from the column within a ph range from 6.0 to 6.6. according t ...	1985	2864684
effect of excitatory amino acids and analogues on [3h]acetylcholine release from amacrine cells of the rabbit retina.	the pharmacology of cholinergic amacrine cells has been further studied by examining the effects of excitatory amino acids and antagonists on [3h]acetylcholine (ach) release from the retinas of anaesthetized rabbits. exposure of the retina to glutamate (5 mm), aspartate (5 mm), kainate (8 microm) and quisqualate (8 microm) abolished the light-evoked release of [3h]ach but increased the spontaneous resting release four- to fivefold. n-methyl-d-aspartate (nmda) (5 mm) in normal krebs bicarbonate m ...	1985	2865360
the effect of mixed-function oxidation of enzymes on their susceptibility to degradation by a nonlysosomal cysteine proteinase.	mixed-function oxidation of escherichia coli glutamine synthetase by ascorbate, oxygen, and iron has previously been shown to cause inactivation of the enzyme and enhanced susceptibility to proteolytic attack by a variety of proteases. one of these proteases, from rat liver, is a high molecular weight cysteine proteinase which does not degrade native glutamine synthetase at neutral ph. although inactive, the oxidized glutamine synthetase preparations used in this study were only partially degrad ...	1985	2867745
action of excitatory amino acids and their antagonists on hippocampal neurons.	intracellular recordings were obtained from guinea pig hippocampal neurons maintained in vitro. current- and voltage-clamp techniques were used to study the effect of microiontophoresis of excitatory amino acid agonists. modification of agonist responses by bath application of known concentrations of antagonist agents was also examined. all agonists used, glutamate, aspartate, n-methyl-d-aspartic acid (nmda), and quisqualate, depolarized hippocampal neurons and caused repetitive firing. nmda was ...	1985	2867826
influenza viruses as lymphocyte mitogens. i. b cell mitogenesis by influenza a viruses of the h2 and h6 subtypes is controlled by the i-e/c subregion of the major histocompatibility complex.	influenza a viruses of the h2, h3, and h6 subtypes function as t cell-independent b cell mitogens for lymphocytes from balb/c mice. lymphocytes from c57bl/10 mice, however, undergo mitogenesis only in response to h3 viruses. the failure of c57bl/10 lymphocytes to respond to h2 and h6 viruses was shown not to reflect a difference in dose-response profile or kinetics of the response, nor was it due to the activity of suppressor t cells. experiments with congenic and recombinant strains of mice est ...	1985	3155540
increased sce inducibility by low doses of methylcholanthrene in lymphocytes obtained from patients with down's disease.	the inducibility of sister-chromatid exchanges (sces) following 3-methylcholanthrene (mc) treatment of normal human peripheral blood lymphocytes and of in vitro cultured normal human embryo fibroblasts, as well as peripheral blood lymphocytes of patients with down's disease and of fibroblasts of an embryo with trisomy 21 has been investigated. 10(-6) m mc treatment increased the frequency of sces by 2.5 in the case of down lymphocytes when compared to the healthy control. the fibroblasts with tr ...	1985	3156274
glucose 1,6-bisphosphate decline in human erythrocytes: possible involvement of phosphoglucomutase pgm2 isoenzymes.	human erythrocytes incubated with various sugars lower their glucose 1,6-bisphosphate (glc-1,6-p2) content, as do haemolysates containing exogenous glc-1,6-p2 incubated with sugar monophosphates (sugar-p). experiments performed with isolated erythrocyte phosphoglucomutase (pgm) isoenzymes indicate that only definite isoenzymatic forms, namely pgm2, are able to consume glc-1,6-p2 during the mutation of sugar-p other than glucose-p. in this process a phosphate group is released from glc-1,6-p2 and ...	1985	3157431
sensory reinnervation of cat peroneus brevis muscle spindles after nerve crush.	results are presented of examining the postcrush sensory reinnervation of cat peroneus brevis muscle spindles previously investigated physiologically by hyde and scott. it is shown that primary and secondary endings were successfully restored in their final form in the early stages of recovery. the primary endings were shorter than normal and had fewer transverse bands; 12% were judged to be hyperinnervated. some secondary endings showed signs of growth through the primary region apparently desi ...	1985	3158372
the composition of the ceramides from human stratum corneum and from comedones.	human epidermal surface lipids were collected by an ethanol wash and the ceramides were quantified by thin-layer chromatography-photodensitometry. six ceramide fractions were isolated and the structural components of each were analyzed in detail. the most unusual of the epidermal ceramides contained a sphingosine base with amide-linked 30- and 32-carbon omega-hydroxyacids and an ester-linked nonhydroxyacid, 41% of which was linoleic acid. the proportion of linoleic acid in the analogous ceramide ...	1985	3158712
calla-positive myeloma: an aggressive subtype with poor survival.	detailed immunotyping was carried out on 21 direct myeloma bone marrow aspirates and eight human myeloma cell lines. four previously untreated common acute lymphoblastic leukemia antigen (calla)-positive myeloma patients were identified and six of eight cell lines (75%) were also positive. calla positivity, as part of an immature b phenotype, was found to correlate with very aggressive clinical disease: median survival six months v 56 months for the calla-negative group.	1985	3159443
short term effect of medroxyprogesterone acetate on the rat intestinal digestive & absorptive functions.		1985	3159660
monoclonal antibodies to outer membrane antigens of vibrio cholerae.	hybridoma-derived monoclonal antibodies were prepared against outer membrane antigens of four strains of vibrio cholerae that were cultivated under iron-limited conditions, and these antibodies were partially characterized. we established a library of 66 hybridomas which produced monoclonal antibodies defining 16 different v. cholerae antigens. two antigens (molecular weights, 18,000 and 112,000) were heat modifiable, whereas the reacting epitope of a third antigen (40,000-dalton-18,000-dalton d ...	1985	3159676
renal apical membrane cholesterol and fluidity in regulation of phosphate transport.	renal proximal tubule cells adapt to dietary phosphate (pi) restriction by increasing pi transport independent of parathyroid hormone, vitamin d metabolites, or serum ca2+. to determine the underlying cellular mechanism(s), brush border (bbm) and basolateral membranes (blm) were isolated from growing male rats fed a synthetic diet containing variable levels of pi (0.1-1.4%). dietary pi restriction was without effect on either bbm or blm total lipid phosphorus, individual phospholipid species, or ...	1985	3160247
direct selection of mutations reducing transcription or translation of the reca gene of escherichia coli with a reca-lacz protein fusion.	when a reca-lacz protein fusion was cloned into phage lambda, the resulting transducing phage grew normally on wild-type escherichia coli, but its growth was severely inhibited in lexa(def) mutant strains that express reca constitutively at high levels. mutants of the transducing phage that grew on the lexa(def) strains were isolated and were found to affect production of the reca-beta-galactosidase hybrid protein. most mutants, including a number of nonsense mutants, were phenotypically lacz-. ...	1985	3160689
the relations between erythrocytic and cns parameters following postnatal hypobaric hypoxia of the rat.	in the present paper the relations between selected erythrocytic criteria and alterations of biochemical brain parameters following hypoxia were investigated in order to find out the relevance of erythrocytic criteria for the diagnosis of hypoxia-induced alterations of the cns during development. newborn wistar rats (n = 80) with their mother were exposed to hypobaric hypoxia, po2 = 11.3 kpa, from day 2-10 of life, daily for 11 h. on the 11th day of life the animals had a lower body (38%), liver ...	1985	3161100
stool cyclic amp in diarrhoea due to different causative organisms.		1985	3016069
detection of dihydroorotate dehydrogenase activity as a marker of de novo nucleic acid synthesis in germinal centers of spleens following antigen administration.	a histochemical technique, developed for the localization of dho-d activity in tissue, has been utilized to study the distribution of this activity in rabbit spleen. this enzyme is involved in the de novo synthesis of pyrimidines of nucleic acids via orotic acid as an intermediate. the enzyme was found to be most active at the periphery of follicles in the resting spleen. following antigenic challenge, enzyme activity became prominent for several days in macrophages within germinal centers, but ...	1985	3023585
activities of deglycosylated thyrotropin at the thyroid membrane receptor-adenylate cyclase system.	a bovine thyrotropin (btsh) preparation was deglycosylated by treatment with anhydrous hydrogen fluoride (hf) in the presence of anisole. the resulting material consisted of tsh derivatives that exhibited different molecular sizes, all smaller than the native hormone. the majority (62%) of the deglycosylated tsh derivatives did not bind to the lectin concanavalin a, while 98% of the native tsh was able to bind. the deglycosylated tsh derivatives bound to the high affinity-high specificity tsh bi ...	1985	3009595
near infrared optical monitoring of intact skeletal muscle during hypoxia and hemorrhagic hypotension in cats.		1985	3008518
genetic basis of trimethoprim and o/129 resistance in vibrio cholerae.	because of its important consequences on prophylaxis and therapy of cholera and on bacterial identification, we have studied the genetic basis of cross-resistance to trimethoprim and o/129 of strains of vibrio cholerae o1 independently isolated in africa. two classes of bacteria were found. in the first class, the strains were also resistant to ampicillin and kanamycin and to high levels of streptomycin by synthesis of a 3"- or 6-aminoglycoside phosphotransferase. the strains hybridized weakly w ...	1985	3008635
the interaction of recombinant huifn-gamma 1 and recombinant huifn-alpha 2 on the 2-5a system in two distinct cell types.		1985	3008171
2-5a analogs as mechanistic probes of the 2-5a system: stereoconfiguration of phosphorothioate analogs of 2-5a markedly influences metabolic stability.	the stereoconfiguration of the diester bond of phosphorothioate analogs of 2-5a strongly influenced lability to enzymatic hydrolysis by cellular and serum phosphodiesterases. bonds containing the sp configuration were extremely resistant to hydrolysis compared to the corresponding rp configuration linkages. the rate of hydrolysis of the diester bond was influenced by chain length of the adenylate oligomer, with a stability ranking of dimer greater than trimer greater than tetramer; as well as by ...	1985	3008174
ergot alkaloids and central monoaminergic receptors.	the interactions of ergolines and of ergopeptines with dopamine (da), alpha 1 and alpha 2 central adrenoreceptors were studied. ergolines and ergopeptines exert agonist activities at central da receptors and exhibit antiparkinsonian activities in monkeys with unilateral ventromedial tegmental lesions of the brain stem. both ergolines and ergopeptines are used in treatment of parkinson's disease and their therapeutic efficacy, as well as their propensity to develop undesirable side effects is und ...	1985	3005775
effects of anethole dithiolthione and 2(3)-tert-butyl-4-hydroxyanisole on schistosome granuloma formation.	administration of the antioxidants 2(3)-tert-butyl-4-hydroxyanisole (bha) or 5-(p-methoxyphenyl)-3h-1,2-dithiol-3-thione (adt) to female cd-1 mice starting 4 weeks after infection with 70 cercariae of schistosoma mansoni resulted in a decrease in the size of the inner fibrotic region of the hepatic granuloma. the cellular composition of the granuloma was not altered by treatment with these two compounds. the administration of the specific superoxide scavenger copper diisopropylsalicylate (cudips ...	1985	3005948
effects of anethole dithiolthione and 2(3)-tert-butyl-4-hydroxyanisole on schistosome granuloma formation.	administration of the antioxidants 2(3)-tert-butyl-4-hydroxyanisole (bha) or 5-(p-methoxyphenyl)-3h-1,2-dithiol-3-thione (adt) to female cd-1 mice starting 4 weeks after infection with 70 cercariae of schistosoma mansoni resulted in a decrease in the size of the inner fibrotic region of the hepatic granuloma. the cellular composition of the granuloma was not altered by treatment with these two compounds. the administration of the specific superoxide scavenger copper diisopropylsalicylate (cudips ...	1985	3005948
effect of pyrimidinone treatment on lethal and immunosuppressive murine cytomegalovirus infection.	the 2-amino-5-halo-pyrimidinones, which are potent interferon inducers and antiviral agents, were found to be protective against lethal cytomegalovirus (cmv) challenge in weanling or neonatal mice when administered before virus challenge. this protection was dependent upon the dosage of pyrimidinone administered. weanling mice infected with a sublethal challenge of cmv exhibited moderate to severe immunosuppression as measured by reduced splenic cell blastogenic responses in vitro to the mitogen ...	1985	3000290
purification of a metalloproteinase inhibitor from human rheumatoid synovial fluid.	a metalloproteinase inhibitor present in human rheumatoid synovial fluid was purified by a combination of heparin-sepharose chromatography, concanavalin a-sepharose chromatography, ion-exchange chromatography and gel filtration. the mr of the purified inhibitor was 28000 by sds/polyacrylamide-gel electrophoresis and 30000 by gel filtration. the inhibitor blocked the activity of the metalloproteinases collagenase, gelatinase and proteoglycanase, but not thermolysin or bacterial collagenase. the s ...	1985	3000352