Publications

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immunosuppressive effect of a naturally acquired subclinical bursal agent infection on vaccination against newcastle disease.a naturally acquired subclinical infection of infectious bursal agent was shown to be the probable causative factor of marked immunosuppression of newcastle disease vaccine in young chicks. old hens maintained in the same contaminated environment did not exhibit immunosuppression.1976186932
response of growing chickens to an inactivated ibd antigen in oil emulsion. 1976188221
[infectious bursal disease: distribution and persistence of the virus in inoculated chickens. study on the transmission of the disease].the distribution, concentration and persistence of infectious bursal disease virus (ibdv) in the lymphoid organs of inoculated chickens, and its persistence in contaminated premises was examined. the virus only multiplied in the bursa of fabricius where it induced degeneration and necrosis of the lymphoid cells. it persisted for 10 days in this organ and the highest viral concentrations were observed between the 4th and 8th day following inoculation. the virus was found at a low concentration in ...197618777327
infectious bursal disease in ghana. 197616300347
infectious bursal disease agent: morphology by negative stain electron microscopy.the virus of infectious bursal disease of chickens was studied by immune electron microscopy. negatively-stained preparations revealed morphological similarities with both the bluetongue virus group, and the virus of infectious pancreatic necrosis of trout. results indicated that the small particle found in such preparations is a degradation product of the large particle.197550057
infectious bursal disease virus: antigen production and immunity. 1975164798
a comparison of gel diffusion, fluorescent antibody and virus isolation methods in experimental and natural cases of infectious bursal disease.in studies with chicks inoculated with the sk-1 strain of infectious bursal agent the bursa of fabricius was found to be the tissue of choice for virus isolation as well as for use in the fluorescent antibody test and the agar gel diffusion test. in separate experiments positive results were obtained until postinoculation days 3 or 4 by the agar gel diffusion test, 5 or 6 by the fluorescent antibody test and 14 by the virus isolation method, respectively. bursas from chickens involved in seven n ...1975164991
immunodeficiency in the chicken. ii. production of monomeric igm following testosterone treatment or infection with gumboro disease.chickens were treated at an early embryonal age with testosterone propionate or infected neonatally with the virus producing gumboro disease. their sera were subsequently fractionated by sephadex g-200 chromatography, and showed a complete deficiency of igg and the presence of igm which was eluted with the 7s protein fraction. purified and 125-i-labelled monomeric igm was examined by sds-acrylamide gel electrophoresis and found to contain both mu and light chains, together with a chain of interm ...1975166037
the effect of diet on the severity of losses from infectious bursal disease (gumboro) in a commercial broiler genetype.a natural outbreak of infectious bursal disease occurred in an experimental broiler flock being reared on 3 starter diets having 18.8, 21.6 and 24.0 per cent protein. groups fed the highest protein starter exhibited significantly higher mortality and had larger numbers of stunted birds.1975166366
structural and growth characteristics of two avian reoviruses.two virus strains which had been suspected to be the etiological agents of infectious bursitis (gumboro disease) and of inclusion body hepatitis of chickens were characterized by their morphology, their peptide composition and the segmented genome of their double-stranded rna to be typical reoviruses. although the 2 avian strains did not clearly differ in their serological behaviour, the size of some of their rna segments were not identical. both strains replicated in tissue cultures prepared fr ...1975170888
comparison of vaccines against infectious bursal disease. 1975171288
letter: quantitation of antibodies to infectious bursal disease. 1975171823
the roles of the infectious bursal agent and several avian adenoviruses in the hemorrhagic-aplastic-anemia syndrome and gangrenous dermatitis.the effect that breeder-flock immune status regarding the infectious bursal agent (iba) and two avian adenoviruses (dpi-1 and dpi-2) has on the susceptibility of their commercially reared delmarva broiler progeny to the hemorrhagic-aplastic-anemia syndrome and concurrent gangrenous dermatitis was determined. lack of immunity to the iba in breeder flocks was related to an increased susceptibility of progeny to anemia and dermatitis. breeder-flock immunity to the two adenoviruses tested could not ...1975173278
an outbreak of infectious bursal disease (ibd) of chickens in nigeria. 1975175539
specific b lymphocyte suppression by infectious bursal agent (gumboro disease virus) in chickens.chickens infected experimentally with infectious bursal agent (iba) during embryonation or early after hatching show a severe depression of bursa-dependent lymphoid components, and associated immune functions. data presented here and elsewhere show that the degree of b cell suppression correlates with the time of infection. this would be compatible with a virus-induced block in b lymphocyte differentiation. accordingly, the virus may be cytopathic for early (bursal) but not late (circulating) b ...197511993333
purification and preliminary characterisation of a pathogenic strain of infectious bursal disease virus.a pathogenic strain of infectious bursal disease virus has been purified by density gradient centrifugation, principally on sucrose and tartrate gradients. examination of gradient peak fractions by electron microscopy has revealed two populations of particles of average size 62 nm and 20 nm, which band together on sucrose and tartrate gradients. purified virus has been shown to reproduce the typical symptoms and lesions of infectious bursal disease. the possible classification of the larger viru ...197518777306
some properties of precipitating antigens associated with infectious bursal disease virus.infection of the bursa of fabricius and chicken embryo fibroblast cell cultures with avian infectious bursal disease virus resulted in production of a number of virus-induced antigens. the antigens were specific, forming three precipitin lines by immunodiffusion with antiserum (designated pa-1, -2, and -3). to separate immunoprecipitin from the remaining viral particles, two (pa-1 and pa-3) were partially purified by subjection to two cycles of diethylaminoethyl-cellulose chromatography and filt ...19744215758
the immunodepressive effect of infectious bursal disease virus in chickens. 19744360708
infectious bursal disease virus: growth and characterization in cell cultures. 19744364080
isolation and identification of infectious bursal disease virus in lebanon. 19744368849
the occurrence of cytopathic effect of an isolate of infectious bursal disease virus. 19744449163
electron-microscope characterization of infectious bursal disease virus. 19744851821
structure of infectious bursal disease virus.infectious bursal disease virus of chickens was purified, and its structure was examined by the negative-staining technique in the electron microscope. the buoyant density of infectious bursal disease virus in cscl was found to be 1.34 g/cm(3). the morphological details suggest that the capsid of the virion consists of a single layer of 32 capsomeres arranged in 5:3:2 symmetry. the virion measured about 55 nm in diameter and had no envelope.19744138194
attenuation of infectious bursal disease virus and vaccination trials under laboratory and field conditions.adaptation to the chicken embryo of a virus strain of infectious bursal disease resulted in a reduction in virulence. after 60 passages the strain was attenuated enough to be used as a vaccine. the attenuation proved adequately stable. if given orally in the drinking water, the strain induced the development of neutralising and precipiting antibodies and a strong resistance against the disease. promising results have also been obtained in the field.197418777257
isolation of infectious bursal disease virus and distribution of precipitating antibodies in chicken sera. 19734200133
infectious bursal disease viral particles revealed in avian cell culture by electron microscope. 19734376189
biological and physico-chemical properties of the infectious bursal disease virus (ibdv).some biological properties of an egg-adapted strain of ibdv, pv1, have been studied. by immunofluorescent staining the strain is serologically identical to another egg-adapted strain, mu2, and to three pathogenic strains of ibdv. all five strains do not react with antisera prepared against known avian viruses of various groups, including reoviruses. the egg-adapted strain pv1 replicates in chick embryo fibro-blasts and in rk13 cells, with the formation of plaques. the pv1 strain, highly neuropat ...197318777392
identification of the infectious bursal disease virus in mexico. 19724622733
infectivity and distribution of infectious bursal disease virus in the chicken. persistence of the virus and lesions. 19724624935
infectivity of infectious bursal disease virus for embryonating eggs. 19704989111
binding of the pathogen receptor hsp90aa1 to avibirnavirus vp2 induces autophagy by inactivating the akt-mtor pathway.autophagy is an essential component of host innate and adaptive immunity. viruses have developed diverse strategies for evading or utilizing autophagy for survival. the response of the autophagy pathways to virus invasion is poorly documented. here, we report on the induction of autophagy initiated by the pathogen receptor hsp90aa1 (heat shock protein 90 kda α [cytosolic], class a member 1) via the akt-mtor (mechanistic target of rapamycin)-dependent pathway. transmission electron microscopy and ...025714412
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