Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| functional correlations between mucosal mast cell activity and immunity to trichinella spiralis in high and low responder mice. | levels of intestinal mast cell protease (imcp) were quantified in serum, gut tissue and in intestinal fluids taken from mice infected with trichinella spiralis during primary and secondary infections. the ability to generate a mast cell response was dependent on the response phenotype of the mouse strain used. the mast cell response in rapid responder mice (nih) occurred sooner and was more pronounced than in either intermediate (swr) and low responder (b1o) mice. this pattern was also reflected ... | 1990 | 2084610 |
| infectivity, reproductive capacity and distribution of trichinella spiralis and t. pseudospiralis larvae in experimentally infected sheep. | twelve merino sheep were experimentally shown to be susceptible to infection with trichinella spiralis or t. pseudospiralis by feeding on infected carcasses of mice or by oral intubation with recovered muscle larvae. the larvae recovered from the sheep showed variable tissue distribution. the diaphragm and tongue were most affected. the viability of the recovered larvae was confirmed by successful passage in mice. the reproductive capacity of t. spiralis in sheep was higher than that of t. pseud ... | 1990 | 2094791 |
| involvement of type i hypersensitivity in rapid rejection of trichinella spiralis from adult rats. | the role of type i hypersensitivity in the rapid rejection of trichinella spiralis from actively and passively immunized rats was examined. net intestinal fluid secretion, which occurs during the rejection of the parasites, was used to verify the expression of local anaphylaxis and was examined for its possible role in the rejection process. worm establishment in the small intestine 30 min after intraduodenal inoculation was significantly reduced in rats that were passively immunized with immune ... | 1990 | 2099353 |
| immunotoxicity of bis(tri-n-butyltin)oxide in the rat: effects on thymus-dependent immunity and on nonspecific resistance following long-term exposure in young versus aged rats. | to investigate whether immune function suppression observed in an earlier study after short-term bis(tri-n-butyltin)oxide (tbto) exposure also occurred after long-term treatment, function studies for specific and nonspecific resistance were performed after exposure of weaned male rats to diets containing 0, 0.5, 5, or 50 mg tbto/kg for 4-6 and 15-17 months. treatment for 4.5 months had no effect on body weight but reduced thymus weight at 50 mg/kg. regarding the thymus-dependent immunity, delaye ... | 1990 | 2118283 |
| immunodiagnosis of nematode infections and prospects for vaccination, with special reference to trichinella spiralis. | within the phylum nematoda are species not only responsible for several chronic, debilitating diseases of man, but also for significant economic losses resulting from parasitaemias of livestock. parasitic zoonoses caused by nematodes, such as trichinosis, present a public health concern as well. in an effort to combat these diseases, much research has been directed towards the development of vaccines that will protect man and animals from selected parasitic infections. concomitantly, sensitive, ... | 1990 | 2132694 |
| dna analysis in the diagnosis of infection and in the speciation of nematode parasites. | dna analysis is playing an increasingly important role in characterising and classifying nematode parasites. though less emphasis has been placed on utilising dna elements to study nematodes of veterinary importance, correct diagnosis is, nevertheless, critical to proper treatment and control. the genus trichinella presents a particularly interesting problem since the level of classification within this genus remains unclear. herein we discuss the application of dna analysis to the diagnosis and ... | 1990 | 2132695 |
| intraintestinal migration to the epithelium of protective, dividing, anti-trichinella spiralis cd4+ ox22- cells requires mhc class ii compatibility. | we have measured the intraintestinal migration of activated th cells belonging to the ox8- ox22- and ox8- ox22+ subsets derived from thoracic duct lymph of rats infected with trichinella spiralis. cells in s-phase were labeled with 125i-udr or 3h-tdr in vitro and transfused i.v. identical proportions of both helper cell subsets localized in the small intestine but three to four times as many ox22- cells as ox22+ cells migrated to the intestinal epithelium. experiments using mhc class i and ii re ... | 1990 | 2142708 |
| t-helper subset function in the gut of rats: differential stimulation of eosinophils, mucosal mast cells and antibody-forming cells by ox8- ox22- and ox8- ox22+ cells. | thoracic duct lymphocytes (tdl) collected 3 days after infection of rats with trichinella spiralis (ts) and adoptively transferred into normal, uninfected recipients, increased the numbers of both mucosal mast cells (mmc) and eosinophils (eos) in the intestine. the cd4+ t-helper cell population was separated into two subsets (ox22+ and ox22-) using ox22 monoclonal antibody (mab) and panning techniques. after adoptive transfer of these t-helper subsets i.v., rats were challenged with ts 24 hr lat ... | 1990 | 2146212 |
| alterations in the longevity and fecundity of adult trichinella pseudospiralis related to method of isolation of infective larvae. | the infectivity of trichinella pseudospiralis infective larvae was reduced significantly following exposure to low ph or a combination of 1% pepsin at low ph compared to that for larvae isolated in phosphate-buffered saline (pbs) at ph 7.0. reduction of host gastric ph by administration to mice of sodium bicarbonate solution in pbs was accompanied by an increase in the infectivity of larvae isolated in 1% pepsin/hcl (p/hcl) compared to that for worms inoculated into hosts given pbs alone. fewer ... | 1990 | 2161917 |
| altered smooth muscle function in worm-free gut regions of trichinella-infected rats. | we have extended a recent study demonstrating increased contractility of jejunal smooth muscle of rats infected with the nematode parasite trichinella spiralis, the primary habitat of which is the jejunum. in this study, muscle from the worm-free ileum of infected rats showed decreased contractility compared with control, illustrating regional differences in muscle responses to the infection. however, in jejunal muscle from worm-free segments that had been excluded from the rest of the gut befor ... | 1990 | 2166442 |
| the effects of ethanol consumption on the expression of immunity to trichinella spiralis in rats. | the effects of ethanol ingestion on immune responses of female rats against trichinella spiralis (t. spiralis) infections were investigated. female rats were pair-fed either ethanol-containing or isocaloric control liquid diets for 68 days, during which time they underwent one pregnancy cycle. for some studies, animals were fed ethanol diets for 68 days beginning prior to pregnancy and continuing through lactation and involution. they were infected on day 7 of involution with 1000 l1 larvae of t ... | 1990 | 2178479 |
| distribution of intestinal mast cell proteinase in blood and tissues of normal and trichinella-infected mice. | a sensitive and specific enzyme-linked immunosorbent assay (elisa) was developed for mouse intestinal mast cell proteinase (imcp). specificity was demonstrated by the absence of immunoreactivity with extracts of isolated serosal mast cells (smc), or with high concentrations (50 micrograms/ml) of the antigenically similar rat mast cell proteinases i or ii. the small and large intestines in normal mice were the major sources of imcp, there being little or no imcp in non-mucosal tissues. concentrat ... | 1990 | 2179829 |
| antigen-antibody complexes in experimental infection with trichinella spiralis and their role in the development of kidney lesions. | the kidneys of rats, experimentally infected with trichinella spiralis were studied by means of light and fluorescent microscopy. the results obtained suggest that this is a case of a proliferative intra- and extracapillary nephritis. highest intensity of the pathological process was noted between the 30th and 50th day of the infection. the immunofluorescent reaction of kidney glomeruli demonstrated that the immune complexes were located predominantly in the basal membrane and the mesangium. the ... | 1990 | 2186668 |
| trichinosis in a dog. | trichinella spiralis infection was identified by direct fecal examination as the cause of gastrointestinal disease in a dog. the source of infection was believed to be a woodchuck. management included supportive care and benzimidazole treatment. vomiting, diarrhea, anorexia, and myalgia are the main signs of trichinosis, but routine fecal examination seldom reveals t spiralis in natural infections. mebendazole is the recommended anthelmintic and should be used to eliminate intestinal larvae and ... | 1990 | 2211292 |
| immunocytochemical evaluation of trichinella spiralis larval antigen. | the antigenicity of trichinella spiralis muscle larvae was studied for the purpose of developing an immunodiagnostic test for trichinosis. ultrathin sections of fixed larvae were treated with sera from patients with a variety of parasitic infections including trichinosis, anisakiasis, gnathostomiasis, fascioliasis, dirofilariasis, severe trichuriasis, moderate trichuriasis, paragonimiasis, cysticercosis cellulosae hominis and diphyllobothriasis, and then treated with protein a-tagged colloidal g ... | 1990 | 2212922 |
| genetic analysis of the relationship between interleukin production and worm rejection in trichinella spiralis-infected inbred mice. | the production of interleukin 1 (il-1), il-2, and il-3 by peritoneal macrophages, mesenteric lymph node (mln), or spleen cells from inbred strains of mice infected with trichinella spiralis was examined. the mice belonged to the worm rejection phenotypes previously characterized as strong (nfs), intermediate (c3h, bub, dba/1, swr, cba, etc.), or weak (b10.q, b10.br, etc.). strong responder nfs mice produced approximately twice as much il-1 as intermediate responder c3heb/fe or weak responder b10 ... | 1990 | 2213413 |
| [acute trichinosis in children. intrafamilial epidemic outbreak in mexico city]. | four cases of trichinosis found in a family living in iztapalapa, mexico, d.f. are reported. the family reported to frequently eat raw sausage originally from villanueva, zacatecas from which trichinella spiralis larvae were isolated. diagnosis was difficult in the first case since no epidemiological history was associated with the patient, besides the fact that the patient showed no signs of eosinophilia and blood tests were negative. the diagnosis in this case was confirmed through muscular bi ... | 1990 | 2222824 |
| 3-methylcholanthrene-induced immunosuppression in mice to trichinella spiralis antigens. | the immunosuppressive effects of in vivo (subcutaneous) exposure to 40 or 80 mg/kg 3-methylcholanthrene (mc) were examined in aryl hydrocarbon hydroxylase (ahh) responsive c57bl/6 (b6) and ahh non-responsive dba/2 (d2) inbred strains of mice. twenty-four hours after treatment with carcinogen or vehicle alone, animals were primed with crude l1 muscle larvae antigen from t. spiralis. immune status was assessed in vitro after six days as antigen-specific lymphoproliferation. the proliferation of sp ... | 1990 | 2229923 |
| pattern of eosinophilia and muscle larval burdens in experimental trichinosis. | absolute eosinophilic counts to different developmental stages of trichinella spiralis in experimental trichinosis were studied to identify the stage responsible for eosinophilia. two peaks of eosinophilia were noticed, the first (545 +/- 172.3) was attributed to the adult stage while the migrating larvae were accused to be responsible for the more pronounced second peak (1265 +/- 331.7). with encapsulation a steady raised level was demonstrated. chromatographic studies to determine the nature o ... | 1990 | 2230342 |
| effect of t. spiralis infection on intestinal motor activity in the fasted state. | we sought to determine the effects of trichinella spiralis infection on small intestinal motor activity in the fasted state in dogs and relate it to clinical symptoms during the intestinal phase of trichinosis. motor activity was recorded by strain gauge force transducers. infection with t. spiralis resulted in a significant increase in the incidence and proximal origination of giant migrating contractions (gmcs) during the first 5 days postinfection. this was also the time when the dogs had dia ... | 1990 | 2240213 |
| helminth parasitism in martens (martes americana) and ermines (mustela erminea) from washington, with comments on the distribution of trichinella spiralis. | helminths are reported for the first time from ermines (mustela erminea) and martens (martes americana) in washington (usa). among 22 adult ermines, 41% were infected by one or more of five species (taenia mustelae, alaria mustelae, molineus patens, m. mustelae and trichinella spiralis). among 78 adult martens from three geographic localities, the prevalence was 83%. nine species were identified (mesocestoides sp., t. mustelae and t. martis americana, euryhelmis squamula, m. patens, baylisascari ... | 1990 | 2250320 |
| isolation of trichinella-specific antigens for diagnosis by gradient monoclonal antibody affinity chromatography. | monoclonal antibodies were generated for the isolation of specific antigens from trichinella spiralis. two monoclonal antibodies (7g6-2 and 10b6-1) of class igg2b and igg1 were selected according to their reactivities in an enzyme-linked immunosorbent assay and western blot. clone 7g6-2 reacted with an antigen with molecular mass of approximately 60 kda, and clone 10b6-1 bound to multiple antigens ranging from 49 to 62 kda on western blot. antibodies of each clone were purified partially from mo ... | 1990 | 2254818 |
| molecular cloning and expression of an immunodominant 53-kda excretory-secretory antigen from trichinella spiralis muscle larvae. | a trichinella spiralis cdna expression library was constructed in lambda gt11 from muscle larvae mrna and immunologically screened to identify genes encoding previously described immunodiagnostic excretory-secretory (es) antigens. screening the library with t. spiralis infection serum from swine or rabbit antiserum to t. spiralis es antigen identified one clone, designated tsa-12, that contains a cdna transcript 539 bp in length and codes for an apparent 123-kda beta-galactosidase fusion protein ... | 1990 | 2270099 |
| a tube latex test based on colour separation for the detection of igm antibodies to either one of two different microorganisms. | a simple two stage assay was developed for the detection of igm antibodies to either one of two microorganisms chosen arbitrarily for this study. salmonella enteritidis and trichinella spiralis. in the first stage, magnetic polystyrene beads (dynabeads) coated with anti-mu (mouse) antibodies were incubated with the test material for 45 min to capture the igm antibodies. in the second stage, indicator latex particles were incubated with the dynabeads for 30 min and the results read following sett ... | 1990 | 2273269 |
| trichinella spiralis: the in situ localization of muscle larva antigens recognized by humans. | 1990 | 2295323 | |
| trichinella spiralis: murine strain variation in response to monoclonally defined, protective, nonstage-specific antigens. | nine hybridoma cell lines secreting monoclonal antibodies (mabs) against trichinella spiralis muscle larvae (ml) excretory/secretory antigens (esa) were developed. two mabs, 6-d8-e3 (6d8) and 6-b1-g10 (6b1), were studied in detail. western blot analysis using ml esa showed that 6d8 recognized 35- and 40-kda constituents whereas 6b1 identified a doublet of 33 kda. however, western blots of sds-page of crude ml homogenate showed that 6d8 identified proteins of approximately 35 and 43-60 kda, where ... | 1990 | 2311715 |
| a thymus-independent (type 1) phosphorylcholine antigen isolated from trichinella spiralis protects mice against pneumococcal infection. | a phosphorylcholine (pc)-containing glycoprotein of 68,000 molecular weight (mw) was isolated from trichinella spiralis. the potential of this antigen (tsp) as a species-specific vaccine against streptococcus pneumoniae was studied in both immunologically deficient (cba/n) and normal (cfw) mice. unlike the pc determinant found in s. pneumoniae, tsp is a type 1 thymus-independent (ti-1) antigen, as it was able to stimulate pc-specific antibody production in cba/n animals, though less well than in ... | 1990 | 2312167 |
| effect of high temperature on infectivity of toxoplasma gondii tissue cysts in pork. | to study the effect of high temperature on infectivity of toxoplasma gondii tissue cysts, pork from infected pigs was mixed with infected mouse brains and homogenized thoroughly. twenty-gram samples of infected homogenized meat were sealed in plastic pouches, pressed to a uniform thickness of 2 mm, and subjected to water-bath temperatures of 49, 52, 55, 58, 61, 64, and 67 c for 0.01, 3, 6, 9, 12, 24, 48, and 96 min. treated samples were digested in hcl-pepsin solution and bioassayed in mice. tox ... | 1990 | 2319420 |
| a spectrum of antibody response with time after trichinella spiralis infection in rats. | a chronology of class-specific antibody response against the trichinella spiralis infection in fischer rats was investigated. g-class antibodies against the cuticle inner layer(s), hypodermis, hemolymph, glycogen aggregates, discrete areas in genital primordial cells, intestinal gland cell granules, and cytoplasmic granules in the cords were detectable 2 wk after infection (the rapid-responding group), whereas g-class antibodies against the cuticle surface, stichocyte granules, and the esophagus ... | 1990 | 2319424 |
| trichinella spiralis: major histocompatibility complex-associated elimination of encysted muscle larvae in swine. | a heretofore undescribed host-mediated reactivity against encapsulated muscle larvae (ml) of the nematode trichinella spiralis is reported. inbred miniature swine (nih minipigs) of three independent sla phenotypes, which received a primary oral dose of 300 t. spiralis ml, successfully resisted a secondary infection of 10,000 ml; however, only pigs of the slaa/a phenotype exhibited an unusual and highly significant reduction in the numbers of encysted ml from the primary infection (p less than 0. ... | 1990 | 2323396 |
| trichinella spiralis: altered expression of muscle proteins in trichinosis. | mammalian muscle undergoes significant alterations morphologically, ultrastructurally, and biochemically following infection by trichinella spiralis larvae. to investigate this host/parasite relationship in more detail, a new method to isolate t. spiralis-infected cells (nurse cells) in preparative quantities was developed. nurse cells isolated by sequential protease treatments contain larvae and retain many of the characteristics of those in situ. when analyzed by sds-page, a wide range of prot ... | 1990 | 2323397 |
| role of t cell subsets in the maternal-to-neonatal transmission of immunity against trichinella spiralis during lactation in rats. | we have previously demonstrated the maternal-to-neonatal transfer of immunity to t. spiralis during lactation and have shown that antigen-specific t lymphocytes, when injected into the mother or orally fed to neonates, can mediate this transfer. to further analyze the t cell subsets involved in conferring this protection, t lymphocytes were isolated from the mesenteric lymph nodes of syngeneic donor rats infected 4-6 days earlier with t. spiralis. the t cells were incubated in vitro with either ... | 1990 | 2329549 |
| effect of some drugs on some histopathological and immunological aspects of experimental trichinosis in albino rats. | the present work is concerned with study of the effects of praziquantel, thiabendazole, mebendazole, cyclophosphamide and cortisone on histopathology of the small intestine (during intestinal phase) and skeletal muscles (during muscular phase), in addition to t. lymphocytes count and serum igg estimation in experimental trichinosis in albino rats. praziquantel has no effect on the histopathology of small intestine or skeletal muscles. thiabendazole and mebendazole treatment resulted in complete ... | 1990 | 2332669 |
| [epidemiology of alveolar echinococcosis in france. 1. intestinal helminths in the red fox (vulpes vulpes l.) from haute-savoie]. | in the course of an epidemiological survey concerning the alveolar echinococcosis and its causative agent, echinococcus multilocularis, the autopsies of 150 wild red foxes captured in the department of the haute-savoie between december 1983 and august 1988 disclosed 139 cases of intestinal helminthiasis with cestodes and nematodes; no trematode was found. multiple infections were frequent. the cestoda recorded consist of the following species: taenia crassiceps: 44 cases (29%); echinococcus mult ... | 1990 | 2344133 |
| trichinella spiralis in an agricultural ecosystem: transmission in the rat population. | four hundred forty-three norway rats (rattus norvegicus) were examined to determine their role in the transmission and maintenance of trichinella spiralis on a pig farm. rats, classified by sex and weight, were examined for trichinellosis by peptic digestion of muscle samples. over a 25-mo period, 188 (42.4%) rats were found to be infected with t. spiralis. the mean intensity of infection was 293.2 larvae per gram (lpg) of muscle; 65 (34.6%) infected rats had intensities of infection greater tha ... | 1990 | 2352066 |
| trichinella spiralis: secreted antigen of the infective l1 larva localizes to the cytoplasm and nucleoplasm of infected host cells. | antibodies were elicited against a purified antigen with an apparent molecular weight of 43k. this antibody preparation also detected a second antigen consisting of a group of closely related components of 45-50k. these antigens are stage specific for the infective first stage larva of trichinella spiralis and are among the repertoire of secreted antigens originating from the stichosome. antibody raised against the 43k antigen reacted with the stichosome and cuticle of the mature larva and the c ... | 1990 | 2354715 |
| synergistic interaction between immune serum and thoracic duct cells in the adoptive transfer of rapid expulsion of trichinella spiralis in adult rats. | rapid expulsion of trichinella spiralis could be transferred to naive adult rats with thoracic duct lymphocytes and immune serum. thoracic duct cells collected from days 3-5 and immune serum collected on day 28, respectively, after infection were effective. both cells and serum were unable to transfer rapid expulsion when given alone, even in large volumes. recipients of immune serum and cells eliminated a significantly higher number of larvae than control rats by 1 hr after challenge with muscl ... | 1990 | 2354718 |
| the role of mucus in antibody-mediated rapid expulsion of trichinella spiralis in suckling rats. | rat pups suckling dams parasitized by trichinella spiralis express rapid expulsion, a protective response that is associated with the entrapment of infectious muscle larvae in intestinal mucus. immunofluorescent studies revealed that antibodies were bound to the surfaces of the entrapped larvae. mucus binding and rapid expulsion occurred in normal pups dosed with larvae coated with antibodies prepared from infected rat serum. subsequent experiments revealed that entrapped larvae escaped from muc ... | 1990 | 2354857 |
| the intestinal gland of trichinella spiralis with emphasis on morphology and antigenicity. | the intestinal gland of muscle larvae and adult worms of trichinella spiralis was investigated with emphasis on its morphology and antigenicity. the gland is situated at the junction between the stichosome and the ampullar portion of the midgut. the cytoplasm was characterized by the presence of cytoplasmic granules. the granules, measuring 1 micron at maximum, were round or sometimes irregular in shape, and of homogeneous appearance with medium to high electron density. rough endoplasmic reticu ... | 1990 | 2358771 |
| zinc deficiency and zinc repletion: effect on the response of rats to infection with trichinella spiralis. | the expulsion of a primary infection of trichinella spiralis was studied in rats fed diets containing (per kg diet) either 3 mg zn [zinc deficient (zn-)] or 40 mg zn [zinc adequate (zn+)]. a dose of 2000 muscle larvae (ml) impaired weight gain (mg/g body wt) in all groups compared with uninfected controls [eg, 0-7 d postinfection (dpi): infected zn-, -105 +/- 10 (means +/- sem); uninfected zn-, 54 +/- 19 (p less than 0.001)]. in a study with 20.5 ml/g body wt, some zn- rats were transferred at t ... | 1990 | 2360546 |
| infectivity, antigenicity and host responses to isolates of the genus trichinella. | comparisons were made of the infectivity and antigenicity of 4 trichinella spiralis isolates (s, d, y, w), of quite different geographical origins, and t. pseudospiralis (p) in rapid- and slow-responder inbred mice. infectivity was measured by the index of reproductive capacity (icr) expressed as the ratio between the number of muscle larvae recovered on day 30 post-infection (p.i.) and the numbers of larvae given at infection. antigen recognition was measured by the degree of proliferation of m ... | 1990 | 2362774 |
| [preparation of monoclonal antibodies against trichinella spiralis and localization of antigens]. | balb/c mice were infected per os with the infective larvae (l1) of trichinella spiralis, and challenged by injecting 0.2ml soluble l1 antigen 3 days before cell fusion. sp2/0 myeloma and immune spleen cells were fused at the ratio of 1:5 in the presence 30%peg (mw 4,000). the fusion rate and antibody positive rate were 92.1% and 16.6% respectively using elisa. among these the chromosomes of 4 strains were 2n greater than 100 and that of the sp2/0 myeloma cells 2n less than or equal to 70. the ti ... | 1990 | 2365331 |
| survey of trichinosis in breeding and cull swine, using an enzyme-linked immunosorbent assay. | serum samples obtained from 40,927 swine at various locations in north carolina between aug 1, 1987 and july 31, 1988, were tested for antibodies to trichinella spiralis, using an elisa based on a larval t spiralis excretory-secretory antigen. in the elisa, samples were considered to have positive results if the optical density (od) reading was equal to or 5 times greater than the mean od value of 4 negative-control sera from trichina-free swine. of the 40,927 serum samples tested, 154 (0.38%) w ... | 1990 | 2368950 |
| pathogenesis and serodiagnosis of experimental trichinella spiralis spiralis and trichinella spiralis nativa infections in cattle. | trichinella spiralis spiralis infections were established in cattle by gavage and by feeding infected musculature in the ration. trichinae were present in greatest numbers in masseter, tongue and diaphragm. trichinella spiralis nativa had a low infectivity to cattle although a light infection was established in one cow by a heavy challenge. cattle had an aversion to eating musculature unless it was camouflaged with molasses. clinical signs of reluctance to eat and masticate were observed between ... | 1990 | 2379115 |
| asymmetric fab glycosylation in guinea-pig igg1 and igg2. | the presence of asymmetric antibody molecules has been investigated in both igg1 and igg2 subclasses of guinea-pig immunoglobulins. it was found that about 20% of the igg1 and 10% of the igg2 were of asymmetric type. the proportion was essentially the same in the sera of normal animals, animals hyperimmunized with dinitrophenyl-bovine gamma globulin (dnp-bgg) and freund's adjuvant, and animals infected with trichinella spiralis. in the case of animals immunized with dnp-bgg, no differences were ... | 1990 | 2379937 |
| biochemical effects of luxabendazole on trichinella spiralis. | biochemical changes produced by luxabendazole in muscle-stage trichinella spiralis larvae consisted of a decrease in free glucose and glycogen levels (46.71% and 35.66%, respectively) after in vivo treatment, slight in vitro inhibition of fumarate reductase activity (24.15%) and, finally, inhibition of [3h]-colchicine-tubulin binding, which was found to be of a competitive nature, with an inhibition constant (ki) of 0.9 x 10(-7) m. in a parallel study, luxabendazole did not appear to be inhibito ... | 1990 | 2381894 |
| partial characterization of two antigens secreted by l1 larvae of trichinella spiralis. | two protein antigens were isolated from excretory-secretory products of trichinella spiralis by biochemical methods and characterized with respect to their chemical and immunological properties. one antigen, of apparent mr 43,000, is an abundant secreted protein of infective l1 larvae, while the other, of 45-50 kda, is present in smaller amounts. yields, extinction coefficients, isoelectric points, amino acid compositions, and partial n-terminal amino acid sequences for each are reported. partia ... | 1990 | 2398916 |
| peripheral blood mononuclear cell subsets during trichinella spiralis infection in pigs. | the immune response of 'yugoslav meat breed' pigs inoculated with low doses of trichinella spiralis muscle larvae was followed over two to nine weeks of primary infection, by analysing changes in peripheral blood mononuclear cell subsets, the development of a humoral antibody response and muscle larvae burden. during the course of the infection, infected animals showed a persistent elevation of both cd4+ and cd8+ t cell subsets from days 15 to 60 after the parasite exposure. during this time, th ... | 1990 | 1974364 |
| the surface lipid of parasitic nematodes: organization, and modifications during transition to the mammalian host environment. | the biophysical properties of the surface lipid of a range of nematode species and their developmental stages were examined, using fluorescent lipid probes and fluorescence recovery after photobleaching (frap). these methods can be applied to living, intact parasites, and the analysis confined to lipid on the outermost surface. in all cases, surface lipid was unusual in its selectivity for the insertion of the lipid probes. in addition, a polar lipid probe was generally not free to diffuse in th ... | 1990 | 1978532 |
| helminths as heirlooms and souvenirs: a review of new world paleoparasitology. | evidence from studies on ancient human feces, intestinal contents and organs of preserved bodies has established that enterobius vermicularis, trichuris trichiura, diphyllobothrium spp and probably trichinella spiralis infected humans in the pre-columbian new world. these species, and perhaps other common human helminths for which there is not yet convincing evidence, probably accompanied transberingeal immigrants and their dogs, and thus can be seen as heirloom parasites. early humans on the am ... | 1990 | 15463308 |
| trichinella spiralis: the worm that would be virus. | trichinella spiralis is one of the world's largest intracellular parasites. unlike most such organisms, it does not kill the host cell, but induces modifications in cell structure that enhance its own survival. in this article, dickson despommier describes the ways in which the parasite uses the cell for its own benefit, and discusses the extent to which this behaviour has contributed to the parasite's success. | 1990 | 15463340 |
| immunological regulation of colonic ion transport. | challenge of distal colonic epithelium from trichinella spiralis-infected guinea pigs with parasite-derived antigen elevated short-circuit current (isc) for approximately 60 min. the maximum elevation (delta isc) was approximately 250 microa/cm2 at 5 min after the addition of trichinella antigen. the antigen-induced alterations in isc were of greater magnitude and duration than those evoked in jejunum. colonic electrical resistance was transiently reduced after exposure to antigen. there was no ... | 1989 | 2465697 |
| trichinella spiralis: antigenic substances associated with the alimentary tract. | postembedding immunogold and immunoperoxidase staining methods revealed that substances occupying the lumen of the esophagus and the midgut were antigenic for both wistar rats and humans. specificity of the alimentary tract-associated antigen was assessed by reacting these substances with a panel of serum pools from patients with non-trichinella helminth infections, including anisakiasis, paragonimiasis, gnathostomiasis, fascioliasis, dirofilariasis, and trichuriasis. the substances had no, or n ... | 1989 | 2470611 |
| trichinella spiralis: recognition of muscle larva antigens during experimental infection of swine and its potential use in diagnosis. | longitudinal studies with trichinella spiralis experimentally infected pigs were carried out to identify muscle larva antigens recognized during infection. this was approached using western blot analysis and elisa assays. immunoblots of sera from experimentally infected pigs using total parasite extracts revealed five principal parasite antigens throughout infection. a similar pattern of antigen recognition was given by sera from backyard pigs in areas of mexico, some of them endemic for trichin ... | 1989 | 2478386 |
| phosphocholine epitopes on helminth and protozoal parasites and their presence in the circulation of infected human patients. | antigens containing phosphocholine (pc) circulate in the blood during chronic filarial infection. because of the wide occurrence of such pc epitopes, we examined their specificity by evaluating 10 common parasites of humans for the presence of pc epitopes, and sera from patients infected with these parasites for circulating antigens containing pc. immunoblot analysis of extracts from various parasites using an anti-pc monoclonal antibody (ca101) demonstrated the presence of pc epitopes on the pr ... | 1989 | 2482559 |
| trichinella spiralis: ultrastructure of the body wall of newborn and muscle larvae. | the body wall structure of muscle and newborn larvae of trichinella spiralis was studied using transmission and scanning electron microscopy. differences were found in the structure of the cuticle of the two developmental stages. in the case of the cuticle of the muscle larvae only transverse striae were present whereas the newborn larvae cuticle showed both transverse and longitudinal striations. in the two parasite stages the outer surface of the cuticle appears as a three-layered structure. t ... | 1989 | 2488769 |
| [trichinosis: new epidemic outbreak caused by the ingestion of wild-boar sausage]. | after the evaluation of 129 serum samples of persons who had ingested boar sausage infested by trichinella spiralis, 48 individuals (40 adults and 8 children) with a mean age of 38.8 years were diagnosed of trichinosis. the incubation time was 17 days (range 2 to 44 days). the following clinical features were outstanding: facial and eyelid edema (50%), diffuse limb myalgia (43%), fever (37%), conjunctivitis (25%), headache (16%), and abdominal pain (16%). remarkably, 33% of the diagnosed patient ... | 1989 | 2490858 |
| preparation and use of monoclonal antibodies in the detection of trichinella spiralis circulating antigen. | in the present study, a trial was made to evaluate the monoclonal antibody produced as a tool in the detection of circulating antigen. for the preparation of monoclonal antibodies, four balb/c mice were immunized with trichinella spiralis larvae. immunized spleen cells were prepared and a suitable mutant ns1 myeloma cell line was used for fusion. eighty white swiss albino mice were orally infected with 500 l1 t. spiralis larvae. their serum was collected at different periods i.e. 5, 11, 18 and 2 ... | 1989 | 2520142 |
| the ige and igg subclass responses of mice to four helminth parasites. | to investigate whether the formation of ige is linked in vivo to an igg subclass, mice were infected with four helminth parasites, nippostrongylus brasiliensis (nbr), mesocestoides corti, taenia crassiceps and trichinella spiralis, and the changes in the serum levels of the different ig isotypes as well as the antibody response to m. corti and t. crassiceps antigen extracts were determined by radioimmunoassays. all four parasites induced a concomitant increase of the ige and igg1 serum levels an ... | 1989 | 2522026 |
| intestinal immunity to trichinella spiralis is a property of ox8- ox22- t-helper cells that are generated in the intestine. | the phenotype of t-helper cells conferring protection against trichinella spiralis (ts) was studied using adoptive transfer procedures and t-helper cell subsets isolated by monoclonal antibodies. with these techniques ox8- ox22+ and ox8- ox22- t-helper cell populations were isolated from thoracic duct lymph (tdl) of infected rats three-five-fold more concentrated than in unfractionated lymph. the ox8- ox22- cell subset alone transferred enhanced rejection of adult worms from the intestine. the o ... | 1989 | 2523861 |
| regeneration in mouse skeletal muscle injured by trichinella larvae. | during the infection caused by trichinella pseudospiralis serious damage to muscles is partly compensated with regeneration process. short, thin fibrillae with central position of the nuclei--regenerating myotubes originate 20--40th day post infection in endomysial tubes remaining after damaged muscle fibres, left by migrating larva. on the 10th day post infection in the vicinity of moving larva activation of nuclei and increased origin of satellite cells under basal membrane occur. with develop ... | 1989 | 2527793 |
| [changes in populations of t lymphocytes in mice infected with trichinella spiralis]. | acid alpha-naphthyl acetate esterase (anae), a cytoplasmic marker was used to identify the t lymphocytes and their subpopulations in peripheral blood of mice experimentally infected with t. spiralis. the results showed that the absolute number of lymphocytes and t lymphocytes increased in parallel on d3 after infection, reaching the peak on d14, and remaining above normal level on d60. the spotted granular anae positive cells (helper t cells, th) were decreasing and the scattered granular anae p ... | 1989 | 2530002 |
| mitochondrial atp-ase as a measure of uncoupling of rat muscle mitochondria in experimental infection with trichinella spiralis and trichinella pseudospiralis. | changes in a bioenergetic state of trichinella spiralis and trichinella pseudospiralis infected rat and mouse muscle mitochondria were evaluated enzymatically, and in both infections 3-4-fold increase of mitochondrial, mg++-stimulated atp-ase (ec 3.6.1.3) was observed. looking for the dynamics of those bioenergetic changes in t. pseudospiralis infected rat and mouse muscle mitochondria 1-2 weeks, pi, the 5-6-fold stimulation of matp-ase activity, followed by a significant drop between the week 3 ... | 1989 | 2530698 |
| evidence for differential induction of helper t cell subsets during trichinella spiralis infection. | the h-2-compatible mouse strains, akr and b10.br, exhibit disparate responses to infection with the parasitic nematode trichinella spiralis. the resistant akr mice expel intestinal adult worms faster than susceptible b10.br mice. we tested antibody and lymphokine responses in these strains. with respect to antibody responses, the b10.br mice had 3- to 10-fold more serum ige and t. spiralis-specific igg1 and iga than akr mice. the b10.br mice also had greater numbers of igg and iga plaque-forming ... | 1989 | 2531779 |
| the relative importance of ah versus h-2 genotype on trichinella resistance following exposure to 3-methylcholanthrene. | the relative influence of ah vs h-2 genotype on the outcome of trichinella spiralis (tsp) infections of mice was examined following methylcholanthrene (mc) treatment. female mice of four inbred strains were treated with mc and infected 24 h later with tsp muscle larvae. the strains, with their respective major histocompatibility complex (mhc) haplotype, aryl hydrocarbon hydroxylase responsiveness (ah phenotype) and level of susceptibility to tsp infection, were: c3heb/fej (c3), h-2k, ahb, tsp su ... | 1989 | 2539339 |
| molecular characterization of sylvatic isolates of trichinella spiralis. | genetic relationships of 20 trichinella isolates from indiana wildlife were assessed and compared to trichinella isolated from an infected swine herd. trichinella larvae were isolated from coyotes, mink, raccoons, and red foxes. the larvae were maintained and amplified in white mice (icr) and wild mice (peromyscus leucopus). differences in phenotypic characters of sylvatic isolates in the 2 laboratory hosts included an approximately 10-30-fold increase in parasite fecundity in wild mice. dna for ... | 1989 | 2542508 |
| altered smooth muscle contraction and sodium pump activity in the inflamed rat intestine. | we examined changes in membrane function underlying the increased contractility of jejunal longitudinal muscle to carbachol in rats infected 6 days previously with trichinella spiralis. muscarinic receptor characteristics were examined in particulate fractions using [n-methyl-3h]scopolamine (nms). there was a significant reduction in the total number of binding sites on muscle from infected rats, but the affinity for nms was unchanged. similarly, in competition studies, the binding of carbachol ... | 1989 | 2552826 |
| trichinella spiralis as a modulator of shope fibroma virus. | after the works on the promoting effect of trichinellosis on some viral infections in rodents, many studies successively demonstrated that trichinella spiralis confers resistance to many unrelated antigens including pathogens, such as protozoa, bacteria and tumour cells (b16 melanoma). considering the above contradictory results, the present work was undertaken to study, in rabbits, t. spiralis as a modulator of shope's fibroma virus, an oncogenic virus responsible for a benign neoplasia. four g ... | 1989 | 2562107 |
| long-term influence of enteric infection on jejunal propulsion in guinea pigs. | the intrinsic fluid-propelling behavior of guinea pig jejunal segments was reportedly altered by primary infection with trichinella spiralis from day 10 to day 20 postinoculation. after that time propulsive behavior returned to normal. the objective in the investigation reported here was to determine if jejunal responsiveness to an infection is influenced by prior exposure to the parasite. accordingly, jejunal propulsion was examined in guinea pigs reinfected 2 mo after the initial inoculation w ... | 1989 | 2583412 |
| antigen-specific t-cell factors induce isotype-like suppression of mast cell and eosinophil-rich t-cell-dependent inflammation in the intestine of mice infected with trichinella spiralis. | the recent identification of a t-cell-derived antigen-binding molecule (tabm), trichinella spiralis factor (tric-f), isolated from culture supernatants of lymphoid cells from mice infected with the intestinal helminth t. spiralis, has led to investigation of the ability of tric-f to induce a t-cell-dependent feedback circuit that ultimately suppresses the production of other tabms with similar (isotype-like) features. this form of regulation that has been identified in contact hypersensitivity a ... | 1989 | 2583851 |
| identification and partial characterization of a t-cell-derived antigen-binding factor from mice infected with the intestinal helminth trichinella spiralis. | immunochemical and biological characterization was performed of an antigen-binding factor derived from culture supernatants of t cells from mice infected 4 days previously with the intestinal helminth trichinella spiralis. affinity chromatography with t. spiralis antigen resulted in the purification of a protein, provisionally designated trichinella factor (tric-f), that shared antigenic and other properties with a known t-cell-derived antigen-binding factor of different antigenic specificity, p ... | 1989 | 2592113 |
| differentiation between trichinella spiralis and t. pseudospiralis infective larvae by a monoclonal antibody. | crude saline extracts of trichinella spiralis and t. pseudospiralis infective larvae were studied by western blot analysis using a monoclonal antibody, named es/ta2 and produced against t. spiralis larvae. this monoclonal antibody recognized seven major antigenic components in t. spiralis larvae with apparent mr: 45, 48, 50, 68, 70, 92 and 105 kda and five in t. pseudospiralis larvae: 38, 50, 70, 72 and 92 kda. sds-page of both extracts did not reveal appreciable differences in the range of mole ... | 1989 | 2600410 |
| piperazine derivatives of benzimidazole as potential anthelmintics. part 1: synthesis and activity of methyl-5-(4-substituted piperazin-1-yl)benzimidazole-2-carbamates. | a series of 5-(4-substituted piperazin-1-yl)-2-nitroanilines (4) and 5-(4-substituted piperazin-1-yl)benzimidazole-2-carbamates (6) has been synthesized starting from 5-chloro-2-nitroaniline (3) and n-monosubstituted piperazines. catalytic reduction of 4 with pd/c followed by treatment with 1,3-dicarbomethoxy-s-methylisothiourea yielded the corresponding methyl-5-(4-substituted piperazin-1-yl)benzimidazole-2-carbamates (6) which were for anthelmintic activity against experimental infections of t ... | 1989 | 2608706 |
| infection of the chinese hamster with trichinella pseudospiralis. | a mean of 2,862 muscle larvae was recovered on day 45 postinfection (pi) from the total body musculature of chinese hamsters infected with 498 trichinella pseudospiralis. infection of the chinese hamster with 494 trichinella spiralis resulted in recovery of a mean of 225 muscle larvae on day 45 pi. the reproductive capacity index for t. pseudospiralis was 5.74, whereas that for t. spiralis was 0.46 in this host species. | 1989 | 2614596 |
| latex agglutination test for detecting trichinella spiralis infections in pigs using muscle extract. | the latex agglutination (la) test, using muscle-juice samples of pigs experimentally infected with trichinella spiralis and slaughtered 95 days post-infection (p.i.), gave visible results in 3 min; even in a pig receiving an infection dose as low as 10 larvae. the test appeared reliable and easy to perform without the need for special equipment or sample treatments which are necessary for ante-mortem diagnostic methods. the muscle-juice sample could be obtained by compressing the muscle pieces w ... | 1989 | 2617824 |
| comparative efficacy of antigen and antibody detection tests for human trichinellosis. | sera collected from patients with suspected or confirmed exposure to trichinella spiralis were tested for circulating parasite antigens and antiparasite antibodies. using an immunoradiometric assay, excretory--secretory antigens from muscle-stage larvae of t. spiralis were detected in the sera of 47% of 62 patients with clinical trichinellosis and 13% of 39 patients without clinical signs but suspected of exposure to infected meat. in comparison, antibodies were detected using an indirect immuno ... | 1989 | 2645393 |
| the use of three immunologic tests in detection of antibodies against trichinella spiralis. | human sera (330 from patients with manifestations suggestive trichinosis) were examined for antibodies. the immunologic tests used were counter current immunoelectrophoresis (cep), enzyme linked immunosorbent assay (elisa) and immunoflourescence (if). seropositive reactions were demonstrated in 2.7%, 5.4% and 2.4% of the sera by the 3 tests respectively. however, 2.4% of the sera were seropositive by the three tests. it seems that the use of two of these tests is valuable in diagnosis when muscl ... | 1989 | 2651538 |
| experimental trichinellosis in horses: biological and parasitological evaluation. | three groups of three horses each were, respectively, infected with 5000, 20,000 and 50,000 larvae of trichinella spiralis. the strain used was isolated from a human biopsy during horsemeat-related outbreaks of trichinellosis in france. transient muscular disorders were only observed in two of the horses infected with 50,000 larvae but none of the horses had fever. a significant increase in blood eosinophils was noticed in 5 horses. serum ldh, aldolase and cpk peaked at the fifth week post-infec ... | 1989 | 2658299 |
| comparative assessment of a double antibody enzyme immunoassay test kit and a triple antibody enzyme immunoassay for the diagnosis of trichinella spiralis spiralis and trichinella spiralis nativa infections in swine. | enzyme immunoassays using the triple antibody enzyme linked immunosorbent assay (elisa) with both trichinella spiralis spiralis and t. spiralis nativa excretory-secretory (es) antigens and a commercial trichinella spiralis enzyme immunoassay test kit were carried out on sera from pigs that were infected with light, moderate and high doses of infective t. spiralis spiralis and t. spiralis nativa respectively. seroconversion occurred in all pigs given infective trichinella larvae although no trich ... | 1989 | 2686832 |
| the genetics of the immune response to trichinella spiralis antigens in the mouse. | 1989 | 2692429 | |
| impaired acetylcholine release from the myenteric plexus of trichinella-infected rats. | we examined the release of acetylcholine (ach) from jejunal longitudinal muscle-myenteric plexus preparations in noninfected control rats and in rats infected 6, 23, or 40 days previously with trichinella spiralis. ach release was assessed by preincubating the tissue with [3h]choline and measuring the evoked release of tritium. the uptake of 3h was significantly less in tissue from t. spiralis-infected rats compared with control. in tissues from either infected or control animals, electrical fie ... | 1989 | 2692462 |
| [immunology of infection by trichinella spiralis sensu stricto]. | the different aspects of immune response to the nematode trichinella are considered. the enteral and parenteral phases of infection are evaluated in detail as regards the contribution of different participants: cells (mast cells, lymphocytes, eosinophils, neutrophils, macrophages), antibodies, complement, inflammation mediators, interleukins etc. the genetic regulation of the immune response, the modulation of the immune system by the parasite and finally the situation as regards vaccination are ... | 1989 | 2698607 |
| ultrastructural localization of antigenic substances in trichinella spiralis. | the in situ localization of antigenic substances in trichinella spiralis muscle larvae was demonstrated at the subcellular level. larvae recovered from mouse muscle were fixed with half-strength karnovsky fixative, dehydrated with alcohol, and embedded in lr white resin. ultrathin sections were incubated with sera from infected wistar rats and, subsequently, protein a-gold complex. the specificity of the immunostaining was confirmed by a control experiment. positively immunostaining structures i ... | 1989 | 2704726 |
| kinetics of iga plasma cells in the intestine of nih mice infected with trichinella spiralis. | in uninfected mice, the percentage of iga-containing cells labelled one day after a single injection of tritiated thymidine [( 3h] d thd) was constant in the upper, middle and lower small intestine. in uninfected mice receiving an additional injection on the two preceding days there was about a threefold increase after the three injections indicating accumulation of labelled cells during the three day period. five days after infection with trichinella spiralis, the percentage of iga cells labell ... | 1989 | 2704883 |
| amino acid sequence of a mouse mucosal mast cell protease. | the amino acid sequence has been determined of a mouse mucosal mast cell protease isolated from the small intestines of mice infected with trichinella spiralis. the active protease contains 226 residues. those corresponding to the catalytic triad of the active site of mammalian serine proteases (his-57, asp-102, and ser-195 in chymotrypsin) occur in identical positions. a computer search for homology indicates 74.3% and 74.1% sequence identity of the mouse mast cell protease compared to those of ... | 1989 | 2706264 |
| experimental trichinosis in alloxan induced diabetes in mice. | the course of trichinella spiralis infection was studied in alloxan induced diabetes in mice. the number of trichinella larvae in muscles of infected animals was estimated by digestion of whole carcases and compared with control non diabetic group. histopathological study of infected muscles was also done. it was found that diabetic mice developed very heavy muscle parasitism and their larval count was high compared with non diabetic animals. besides, there was heavy inflammatory cellular reacti ... | 1989 | 2708851 |
| [intrastrain heterogeneity of trichinella spiralis: a sedimentation analysis]. | intrastrain heterogeneity of muscular larvae of trichinellids has been revealed in experiments of free sedimentation and isopyknic division in the density gradient of saccharose, the presence of which is confirmed by the study of morphological characters and infection activity. the presence of intrastrain heterogeneity indicates the necessity of introduction of standard technique for studies of biological characters of different strains of trichinellids. | 1989 | 2717203 |
| a preliminary study on the use of enzyme-linked immunosorbent assay (elisa) for detection of trichinella spiralis infections in dogs. | an enzyme-linked immunosorbent assay (elisa) was developed to detect trichinella spiralis infections in dogs using rabbit anti-canine igg-horseradish peroxidase prepared according to the improved periodate method and an antigen purified from t. spiralis larvae by sephadex g-200 chromatography. sixty-six canine sera were tested for trichinosis by the elisa and it showed a detection rate which was significantly higher than that by trichinoscopy. this antigen of t. spiralis appeared not to cross-re ... | 1989 | 2741302 |
| complement activation, phagocytosis, tumor growth and parasitic infection after magnesium supplementation in diet of mice. | very little study has been devoted to mg2+ supplementation in the diet and the effects produced upon biological functions. in the present study, mice were given supplemental amounts of mgcl2 or mgso4 in the feed ration, while the following were examined: activation of serum complement, phagocytosis, growth of tumor transplants and infections with trichinella spiralis. increased complement activation, increased phagocytosis, decreased tumor growth and decreased severity of parasitic infection wer ... | 1989 | 2755216 |
| [morphometric and cytologic methods of assessing the host's immune response to invasion by trichinella spiralis]. | morphometric and cytological methods of assessment of the immune response of the host's lymphoid organs to invasion were assayed in experimental model of the white mice trichinelliasis. high informative value allowing to reveal the real processes, causing immune system's alterations in case of trichinelliasis as well as the terms and sequence of appearance of immune responses in various organs, were established. authors suggested methodological approaches to morphometry use and its application i ... | 1989 | 2755404 |
| activation and release of enzymes and major basic protein from guinea pig eosinophil granulocytes induced by different inflammatory stimuli and other substances. a histochemical, biochemical, and electron microscopic study. | in order to investigate the availability and release of enzymes from eosinophilic granulocytes in response to a variety of stimuli, guinea pig peritoneal eosinophils were obtained after repeated intraperitoneal injections of freeze-dried trichinella spiralis larvae. the activities of the enzymes peroxidase, arylsulfatase b, beta-glucuronidase, aminopeptidase, histaminase, cytochrome c oxidase, acid phosphatase, adenosine triphosphatase and glucose 6-phosphatase, and the major basic protein (mbp) ... | 1989 | 2759682 |
| trichinosis in the canadian arctic: report of five outbreaks and a new clinical syndrome. | trichinosis is a serious but understudied medical problem in the arctic. forty-nine consecutive cases in the inuit population of northeastern canada are described. most developed the disease after eating raw walrus, and the clinical presentation of most of these cases differed from previously reported descriptions of classic trichinosis due to trichinella spiralis. unlike the classic syndrome of a brief period of diarrhea followed by fever, myalgia, muscle weakness, and edema, the most common pr ... | 1989 | 2760502 |
| lack of correlation between in vitro and in vivo activation of complement in mice by infective trichinella spiralis larvae. | very little is known about the role played by complement in vivo during trichinella spiralis infections, although previous reports indicate that it binds readily to the surfaces of muscle stages of the parasite in vitro. in order to study the binding of complement to muscle-stage larvae in vivo, larvae were recovered from balb-c inbred, nfr/n inbred, and swiss white outbred mice from 20 to 95 days postinfection. the presence of c3 was examined by direct immunofluorescence and leucocyte- and eryt ... | 1989 | 2760778 |
| characterization of the ribosomal dna from trichinella spiralis. | ribosomal dna (rdna) from three isolates of trichinella was cloned into phage and sublcloned into the plasmid pbr322. the basic repeat unit of rdna was variable in size, with the mapped clones ranging from 10-12 kb. there were differences in restriction sites within the genic region among the three isolates which were due to variations in the internal transcribed region (its) and the intergenic spacer (igs). three rsai sites were mapped to the igs repeat unit of the isolate af1, and one rsai sit ... | 1989 | 2761574 |
| protection against trichinella spiralis induced by purified stage-specific surface antigens of infective larvae. | the stage-specific surface antigens of the infective larvae of trichinella spiralis, isolated using an affinity column of monoclonal antibody (mab) nim-m1, consisted of four components with molecular weights of 72, 65, 52, and 47 kda, respectively. these four components may have unique as well as shared structural features and appear to be products of the stichosome. when injected i.p. as an emulsion in complete freund's adjuvant, the purified antigens induced protection against infection of bal ... | 1989 | 2771924 |
| experimental and theoretical studies of anthelmintics: oxfendazole and its imidazo[1,2-a]pyridine-2-carbamate isomer. | mice experimentally infected with trichinella spiralis were used to test the therapeutic effectiveness of an anthelmintic, methyl 6-(phenylsulfinyl)imidazo[1,2-a]pyridine-2-carbamate, against the immature and adult worms during the intestinal phase of infection. a single oral dose of 100 mg kg-1 of the drug on the third day after exposure to infection was totally ineffective against the adult worms as determined at necropsy on day 6. neither higher unit dosages of the drug, division of the daily ... | 1989 | 2777458 |
| a rapid method for detecting trichinella spiralis larvae in pork using a monoclonal antibody-latex conjugate. | a simple, non-microscopic method was developed for the direct detection of trichinella spiralis in pork. samples of meat were treated with pepsin for 2 h to liberate the larvae, and sonicates were made from the deposit and used in an antigen detection system. in this system, latex particles, sensitized with a parasite-specific monoclonal antibody, were utilized in a slide or tube agglutination test. the efficiencies of these immunological methods were compared with that of trichinelloscopy in th ... | 1989 | 2781716 |
| il-2 production, il-2 receptor expression, and il-2 responsiveness of spleen and mesenteric lymph node cells from inbred mice infected with trichinella spiralis. | the in vitro production of il-2 and il-2r expression by lymphoid cells of inbred mice of strong (nfs), intermediate (c3h), or weak (b10.br) in phenotype of trichinella spiralis (ts) rejection was measured during a primary infection. maximum production of il-2 by spleen and mesenteric lymph node (mln) cells occurred at 5 days postinfection. cell depletion experiments demonstrated that lyt-1.2+ t cells were predominantly responsible for in vitro il-2 production. cells from strong-responder nfs mic ... | 1989 | 2785138 |
| genetic control of eosinophilia. analysis of production and response to eosinophil-differentiating factor in strains of mice infected with trichinella spiralis. | bone marrow cultures were established from mice undergoing parasitic eosinophilia after infection with trichinella spiralis. in the presence of eosinophil-differentiation factor (edf/il-5) eosinophil precursor cells differentiated and could be identified and counted after a 7-day in vitro culture period. the edf-bone marrow assay system was used to determine differences in bone marrow eosinophil precursor capacity between a number of inbred strains of mice. bone marrow cultures from high periphe ... | 1989 | 2788537 |
| a mechanism for anti-asialo gm 1 antibody-induced anaphylactoid response in mice infected with trichinella pseudospiralis. | intravenous injection of anti-asialo gm 1 antibody into mice infected with trichinella pseudospiralis resulted in rapid acute illness or death accompanied by a dramatic rise in hematocrit values in these animals. the described antibody-induced changes were reversible by intravenous infusion of hanks' balanced salt solution (hbss). these effects were not seen in uninfected mice or in trichinella spiralis-infected mice injected with anti-asialo gm 1 antibody. viability of t. spiralis or t. pseudos ... | 1989 | 2795381 |