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complete genome sequences of vb_lmos_188 and vb_lmos_293, two bacteriophages with specificity for listeria monocytogenes strains of serotypes 4b and 4e.listeria monocytogenes is responsible for the rare disease listeriosis, which is associated with the consumption of contaminated food products. we report here the complete genome sequences of vb_lmos_188 and vb_lmos_293, phages isolated from environmental sources and that have host specificity for l. monocytogenes strains of the 4b and 4e serotypes.201525858822
multiplexed detection of foodborne pathogens based on magnetic particles.this paper addresses the novel approaches for the multiplex detection of food poisoning bacteria, paying closer attention to three of the most common pathogens involved in food outbreaks: salmonella enterica, escherichia coli o157:h7 and listeria monocytogenes. end-point and real-time pcr, classical immunological techniques, biosensors, microarrays and microfluidic platforms, as well as commercial kits for multiplex detection of food pathogens will be reviewed, with special focus on the role of ...201525858812
effect of sodium-alginate and laminaran on salmonella typhimurium infection in human enterocyte-like ht-29-luc cells and balb/c mice.brown algal polysaccharides such as alginate, polymers of uronic acids, and laminaran, beta-1,3 and 1,6-glucan, can be fermented by human intestinal microbiota. to evaluate the effects of these polysaccharides on infections caused by food poisoning pathogens, we investigated the adhesion and invasion of pathogens (salmonella typhimurium, listeria monocytogenes and vibrio parahaemolyticus) in human enterocyte-like ht-29-luc cells and in infections caused in balb/c mice. both sodium na-alginate an ...201525857966
plasticity of listeriolysin o pores and its regulation by ph and unique histidine [corrected].pore formation of cellular membranes is an ancient mechanism of bacterial pathogenesis that allows efficient damaging of target cells. several mechanisms have been described, however, relatively little is known about the assembly and properties of pores. listeriolysin o (llo) is a ph-regulated cholesterol-dependent cytolysin from the intracellular pathogen listeria monocytogenes, which forms transmembrane β-barrel pores. here we report that the assembly of llo pores is rapid and efficient irresp ...201525854672
farm to fork quantitative risk assessment of listeria monocytogenes contamination in raw and pasteurized milk cheese in ireland.the objective of this study was to model and quantify the level of listeria monocytogenes in raw milk cheese (rmc) and pasteurized milk cheese (pmc) from farm to fork using a bayesian inference approach combined with a quantitative risk assessment. the modeling approach included a prediction of contamination arising from the farm environment as well from cross-contamination within the cheese-processing facility through storage and subsequent human exposure. the model predicted a high concentrati ...201525850713
odor fingerprinting of listeria monocytogenes recognized by spme-gc-ms and e-nose.microorganisms can produce species-specific microbial volatile organic compounds (mvocs), or odor compounds, which can be characterized by odor fingerprinting. the objective of this study was to characterize the odor fingerprint of listeria monocytogenes. solid-phase microextraction-gas chromatography-mass spectrometry (spme-gc-ms) and electronic nose (e-nose) were used to recognize the mvocs of l. monocytogenes in pure culture medium. the main mvocs of l. monocytogenes were identified by spme-g ...201525847596
stochastic modelling of listeria monocytogenes single cell growth in cottage cheese with mesophilic lactic acid bacteria from aroma producing cultures.a stochastic model was developed for simultaneous growth of low numbers of listeria monocytogenes and populations of lactic acid bacteria from the aroma producing cultures applied in cottage cheese. during more than two years, different batches of cottage cheese with aroma culture were analysed for ph, lactic acid concentration and initial concentration of lactic acid bacteria. these data and bootstrap sampling were used to represent product variability in the stochastic model. lag time data wer ...201525847186
effect of filling type and heating method on prevalence of listeria species and listeria monocytogenes in dumplings produced in poland.the count of listeria monocytogenes was determined, before and after heat treatment, in 200 samples of dumplings of 9 brands and with different types of stuffing. analyses were conducted according to iso 11290-1 standard and with real-time pcr method. the highest count of l. monocytogenes was found in meat dumplings (10(2) to 10(4) cfu/g), whereas products with white cheese-potato stuffing and vegetable-mushroom stuffing contained significantly less listeria, 20 to 80 and 5 to 32 cfu/g, respecti ...201525847074
effect of sulfur dioxide fumigation on survival of foodborne pathogens on table grapes under standard storage temperature.we examined the fate of listeria monocytogenes, escherichia coli o157:h7, and salmonella enterica thompson inoculated on freshly-harvested table grapes under standard cold storage with initial and weekly sulfur dioxide (so2) fumigation. l. monocytogenes and s. enterica thompson were much more sensitive to cold temperature than e. coli o157:h7. furthermore, l. monocytogenes was highly susceptible to so2. initial fumigation with 100 or 200 ppm-hr was sufficient to eliminate this pathogen on grapes ...201525846930
effect of ripeness stage during processing on listeria monocytogenes growth on fresh-cut 'conference' pears.there are several factors that affect the shelf life of fresh-cut fruit, including the cultivar, the ripeness stage of the fruit during processing and the fruit's storage atmosphere and temperature. the effect of fruit ripeness during processing on the survival and growth of listeria monocytogenes on fresh-cut 'conference' pear slices at different temperatures (5, 10 and 20 °c) was studied. the four ripeness stages studied in this work (assessed by a fruit's firmness) were mature-green (54-60 n) ...201525846921
management of listeria monocytogenes in fermented sausages using the food safety objective concept underpinned by stochastic modeling and meta-analysis.in the present work, a demonstration is made on how the risk from the presence of listeria monocytogenes in fermented sausages can be managed using the concept of food safety objective (fso) aided by stochastic modeling (bayesian analysis and monte carlo simulation) and meta-analysis. for this purpose, the icmsf equation was used, which combines the initial level (h0) of the hazard and its subsequent reduction (σr) and/or increase (σi) along the production chain. each element of the equation was ...201525846913
predicting pathogen-specific cd8 t cell immune responses from a modeling approach.the primary cd8 t cell immune response constitutes a major mechanism to fight an infection by intra-cellular pathogens. we aim at assessing whether pathogen-specific dynamical parameters of the cd8 t cell response can be identified, based on measurements of cd8 t cell counts, using a modeling approach. we generated experimental data consisting in cd8 t cell counts kinetics during the response to three different live intra-cellular pathogens: two viruses (influenza, vaccinia) injected intranasall ...201525846273
two-component-system histidine kinases involved in growth of listeria monocytogenes egd-e at low temperatures.two-component systems (tcss) aid bacteria in adapting to a wide variety of stress conditions. while the role of tcs response regulators in the cold tolerance of the psychrotrophic foodborne pathogen listeria monocytogenes has been demonstrated previously, no comprehensive studies showing the role of tcs histidine kinases of l. monocytogenes at low temperature have been performed. we compared the expression levels of each histidine kinase-encoding gene of l. monocytogenes egd-e in logarithmic gro ...201525841007
aerobic plate counts and atp levels correlate with listeria monocytogenes detection in retail delis.listeria monocytogenes is a foodborne pathogen that causes an estimated 1,591 cases of illness and 255 deaths annually in the united states, the majority of which are attributed to ready-to-eat deli meats processed in retail delis. because retail delis distribute product directly to consumers, rapid methods to validate cleaning and sanitation are needed to improve retail food safety. this study investigated the relationships among atp levels, standard aerobic plate count (apc), and l. monocytoge ...201525836412
growth inhibition of listeria monocytogenes, salmonella enterica, and escherichia coli o157:h7 by d-tryptophan as an incompatible solute.under osmotic stress, bacterial cells uptake compatible solutes such as glycine-betaine to maintain homeostasis. it is unknown whether incompatible solutes exist that are similar in structure to compatible solutes but have adverse physiological effects on bacterial physiology. the objective of this study was to evaluate solute incompatibility of various amino acids against bacterial growth. bacterial growth was evaluated by changes in optical density at 595 nm in peptone-yeast-glucose (pyg) brot ...201525836411
analysis of older adults' domestic kitchen storage practices in the united kingdom: identification of risk factors associated with listeriosis.increased listeriosis incidence among older adults (≥ 60 years) has been reported internationally, with many cases reported to be sporadic and associated with ready-to-eat (rte) food products with extended refrigerated shelf life. given that the home kitchen is recognized as a significant location where foodborne illnesses are acquired, it is important that consumers implement safe food practices to minimize risks. this is crucial for vulnerable consumers, such as older adults. consumer food saf ...201525836399
evaluation of novel micronized encapsulated essential oil-containing phosphate and lactate blends for growth inhibition of listeria monocytogenes and salmonella on poultry bologna, pork ham, and roast beef ready-to-eat deli loaves.essential oils and their constituents are reported to possess potent antimicrobial activity, but their use in food processing is limited because of low solubility in aqueous systems and volatilization during processing. two proprietary noncommercial essential oil-containing phosphate blends were evaluated for antimicrobial activity against salmonella enterica cocktail (sc)-and listeria monocytogenes (lm)-inoculated deli meat products made from pork, poultry, or beef. four treatments were tested ...201525836394
serotypes and pulsotypes diversity of listeria monocytogenes in a beef-processing environment.utensils and equipment from meat-processing facilities are considered relevant cross-contamination points of listeria monocytogenes to foods, demanding tracking studies to identify their specific origins, and predict proper control. the present study aimed to detect l. monocytogenes in a beef-processing facility, investigating the diversity of serotypes and pulsotypes in order to identify the possible contamination routes. surface samples from knives (n=26), tables (n=78), and employees hands (n ...201525835809
efficacy of cell wall-deficient spheroplasts against experimental murine listeriosis.various strategies adapted to develop an efficient vaccine against foodborne pathogen, listeria monocytogenes, have met with little success. spheroplasts (bacterial cell devoid of cell wall) are likely to undergo membrane-membrane fusion, leading to the delivery of their content to the cytosol of antigen-presenting cells, thus facilitating mhc class i antigen processing and presentation. in this study, we evaluated the prophylactic potential of listeria spheroplast-based vaccine against experime ...201525833403
colovesical fistula presenting as listeria monocytogenes bacteraemia.we present a case of colovesical fistula presenting with a clinical syndrome of urosepsis subsequently demonstrated to be due to listeria monocytogenes bacteraemia. the patient had a history of previous rectal cancer with a low anterior resection and a covering ileostomy that had been reversed 6 months prior to this presentation. l. monocytogenes was also isolated among mixed enteric organisms on urine culture. there were no symptoms or signs of acute gastrointestinal listeriosis or meningoencep ...201525827919
genetic characteristics of japanese clinical listeria monocytogenes isolates.listeria monocytogenes causes foodborne illnesses through consumption of ready-to-eat foods. although 135-201annual listeriosis cases have been estimated in japan, the details regarding the clinical isolates such as infection source, virulence level, and other genetic characteristics, are not known. in order to uncover the trends of listeriosis in japan and use the knowledge for prevention measures to be taken, the genetic characteristics of the past human clinical isolates needs to be elucidate ...201525826318
bacteriophage predation promotes serovar diversification in listeria monocytogenes.listeria monocytogenes is a bacterial pathogen classified into distinct serovars (svs) based on somatic and flagellar antigens. to correlate phenotype with genetic variation, we analyzed the wall teichoic acid (wta) glycosylation genes of sv 1/2, 3 and 7 strains, which differ in decoration of the ribitol-phosphate backbone with n-acetylglucosamine (glcnac) and/or rhamnose. inactivation of lmo1080 or the dtdp-l-rhamnose biosynthesis genes rmlacbd (lmo1081-1084) resulted in loss of rhamnose, where ...201525825127
the human p-glycoprotein transporter enhances the type i interferon response to listeria monocytogenes infection.human multidrug efflux transporters are known for their ability to extrude antibiotics and toxic compounds out of cells, yet accumulating data indicate they have additional functions in diverse physiological processes not related to drug efflux. here, we show that the human multidrug transporter p-glycoprotein (p-gp) (also named mdr1 and abcb1) is transcriptionally induced in the monocytic cell line thp-1 upon infection with the human intracellular bacterial pathogen listeria monocytogenes. nota ...201525824830
identification of a peptide-pheromone that enhances listeria monocytogenes escape from host cell vacuoles.listeria monocytogenes is a gram-positive facultative intracellular bacterial pathogen that invades mammalian cells and escapes from membrane-bound vacuoles to replicate within the host cell cytosol. gene products required for intracellular bacterial growth and bacterial spread to adjacent cells are regulated by a transcriptional activator known as prfa. prfa becomes activated following l. monocytogenes entry into host cells, however the signal that stimulates prfa activation has not yet been de ...201525822753
the listeria monocytogenes strain 10403s biocyc database.listeria monocytogenes is a food-borne pathogen of humans and other animals. the striking ability to survive several stresses usually used for food preservation makes l. monocytogenes one of the biggest concerns to the food industry, while the high mortality of listeriosis in specific groups of humans makes it a great concern for public health. previous studies have shown that a regulatory network involving alternative sigma (σ) factors and transcription factors is pivotal to stress survival. ho ...201525819074
tumor necrosis factor-α, interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in ossimi sheep with encephalitic listeriosis.encephalitic listeriosis in sheep is a life-threatening disease. however, little is known about the cytokine response and their predictive value in this disease. the aim of present study was to assess the prognostic significance of tumor necrosis factor-α (tnf-α), interleukin-12(p40) (il-12 p40), interleukin-6 (il-6), and interleukin 10 (il-10) levels in cerebrospinal fluid (csf) in sheep with encephalitic listeriosis. fifty-nine ewes in 14 flocks were diagnosed clinically as having listeriosis. ...201525817420
[morphologic changes in listeria cells under the effect of metabolites of human intestinal microflora enterococci].characterization of morphologic changes in listeria cells under the effect of metabolites of human intestine microflora enterococci.201525816518
ascorbic acid, ultraviolet c rays, and glucose but not hyperthermia are elicitors of human β-defensin 1 mrna in normal keratinocytes.hosts' innate defense systems are upregulated by antimicrobial peptide elicitors (apes). our aim was to investigate the effects of hyperthermia, ultraviolet a rays (uva), and ultraviolet c rays (uvc) as well as glucose and ascorbic acid (aa) on the regulation of human β-defensin 1 (defb1), cathelicidin (camp), and interferon-γ (ifng) genes in normal human keratinocytes (nhk). the indirect in vitro antimicrobial activity against staphylococcus aureus and listeria monocytogenes of these potential ...201525815330
a naturally occurring prfa truncation in a listeria monocytogenes field strain contributes to reduced replication and cell-to-cell spread.listeria (l.) monocytogenes is an environmental bacterium that may become an intracellular pathogen upon ingestion to cause gastroenteritis, septicaemia, abortions, and/or fatal infections of the central nervous system. we here describe a l. monocytogenes field strain (jf5171) isolated from a bovine placenta in the context of abortion, which exhibited attenuation in bovine brain-slice cultures. the whole genome of strain jf5171 was sequenced, and the invasion, replication, and intercellular spre ...201525813546
characterization of listeria monocytogenes from three countries and antibiotic resistance differences among countries and listeria monocytogenes serogroups.a total of 104 listeria monocytogenes isolates from 330 fish samples from three countries were characterized by multiplex pcr for serogrouping and virulence markers determination and tested for antibiotics resistance. a 53·8% of the isolates belonged to serogroup 1/2a, 3a; 32% belonged to 1/2b, 3b, 7; 14·4% belonged to 4b, 4d, 4e and 1% belonged to 1/2c, 3c. all isolates exhibited resistance to at least one antibiotic but the resistance rates varied among countries. the isolates exhibited high r ...201525808878
antimicrobial effects of essential oils, nisin, and irradiation treatments against listeria monocytogenes on ready-to-eat carrots.the study aimed at using essential oil (eo) alone or combined eo with nisin and low dose γ-irradiation to evaluate their antibacterial effect against listeria monocytogenes during storage of carrots at 4 °c. minicarrots were inoculated with l. monocytogenes at a final concentration of approximately 7 log cfu/g. inoculated samples were coated by nisin at final concentration of 10(3) international unit (iu)/ml or individual mountain savory eo or carvacrol at final concentration of 0.35%, w/w) or n ...201525807882
division of human listeria monocytogenes pulsed-field gel electrophoresis (pfge) types belonging to lineage i (serovar 4b, 1/2b, and 3b) into pfge groups.the 63 pulsed-field gel electrophoresis (pfge) types identified among 427 clinical isolates of listeria monocytogenes that were characterized in a previous study by serotyping and pfge (asci) could be further divided into 17 pfge groups. while the 63 pfge types, all part of lineage i, were established based on the number and distribution of all bands in each dna profile, the 17 pfge groups were based on the configuration of small bands with sizes <145.5 kb. the 30 pfge types of l. monocytogenes ...201525803595
pneumonia by listeria monocytogenes: a common infection by an uncommon pathogen.infections by listeria monocytogenes typically occur in infants, the elderly, pregnant women, and immunosuppressed subjects. pulmonary infections in adults are extremely uncommon and only few reports can be found in the literature. we here report a case of listeria pneumonia in an 85-year-old female patient and we discuss our diagnostic and therapeutic approach. despite being rare and in most cases difficult to be identified, listeria pneumonia should always be considered in immunosuppressed pat ...201525802774
differences in the expression of cold stress-related genes and in the swarming motility among persistent and sporadic strains of listeria monocytogenes.the persistence of certain listeria monocytogenes strains in food-related environments suggests niche adaptation of these strains and therefore constitutes a major risk to consumer health and results in economic losses for the food producer. in this study, a set of 23 l. monocytogenes isolates, including a group of persistent and a group of sporadic strains, was evaluated regarding their swarming motility at 11°c. in each group, significant (p<0.05) differences in motility were observed. the tra ...201525974395
combined effects of organic acids and salt depending on type of acids and pathogens in laboratory media and acidified pickle.in this study, the effectiveness of combining each of seven types of acids with 3% salt as a treatment against pathogens was investigated in laboratory media and acidified food.201525973744
immersion infection of germ-free zebrafish with listeria monocytogenes induces transient expression of innate immune response genes.zebrafish, denio rerio, can be an alternative to other classic animal models for human infectious diseases to examine the processes of microbial infections and host-pathogen interactions in vivo because of their small body dimension but large clutch size. we established germ-free zebrafish infection models of listeria monocytogenes through different routes of infection: oral immersion and injection via yolk sac, brain ventricle and blood island. immersion of zebrafish larva even with 10(10) cfu/ ...201525972853
cd81 controls immunity to listeria infection through rac-dependent inhibition of proinflammatory mediator release and activation of cytotoxic t cells.despite recent evidence on the involvement of cd81 in pathogen binding and ag presentation by dendritic cells (dcs), the molecular mechanism of how cd81 regulates immunity during infection remains to be elucidated. to investigate the role of cd81 in the regulation of defense mechanisms against microbial infections, we have used the listeria monocytogenes infection model to explore the impact of cd81 deficiency in the innate and adaptive immune response against this pathogenic bacteria. we show t ...201525972472
strain-specific interactions of listeria monocytogenes with the autophagy system in host cells.listeria monocytogenes is an intracellular bacterial pathogen that can replicate in the cytosol of host cells. these bacteria undergo actin-based motility in the cytosol via expression of acta, which recruits host actin-regulatory proteins to the bacterial surface. l. monocytogenes is thought to evade killing by autophagy using acta-dependent mechanisms. acta-independent mechanisms of autophagy evasion have also been proposed, but remain poorly understood. here we examined autophagy of non-motil ...201525970638
a rapidly fatal sepsis caused by listeria monocytogenes type-4b in a patient with chronic renal failure.listeria monocytogenes is a significant zoonosis causing invasive infections in the susceptible persons. the current paper presented a patient who died due to a rapidly-progressing multiple organ failure (mof) as a result of severe sepsis caused by l. monocytogenes.201525969704
expression and function analysis of two naturally truncated myd88 variants in the pacific oyster crassostrea gigas.myeloid differentiation factor 88 (myd88) is the classic signaling adaptor that mediates toll/interleukin-1 receptor (tir/il-1r) dependent activation of nuclear factor-kappa b (nf-κb). in this study, two naturally truncated myd88 members were identified from the pacific oyster (crassostrea gigas), namely cgmyd88-t1 and cgmyd88-t2. the full-length cdna of cgmyd88-t1, cgmyd88-t2 are 976 bp and 1038 bp in length, containing an orf of 552 bp and 555 bp, respectively. the two orf encode a putative pr ...201525963623
prevalence and antimicrobial susceptibility profile of listeria species from ready-to-eat foods of animal origin in gondar town, ethiopia.listeriosis, mostly caused by listeria monocytogenes species, has become a major concern to public health authorities due to its clinical severity and high mortality rate, particularly in high risk groups. currently, there is limited information regarding the prevalence and antimicrobial susceptibility profiles of listeria species in ready-to-eat foods of animal origin in gondar town, ethiopia. the aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of list ...201525963134
are nectarines to blame? a case report and literature review of spontaneous bacterial peritonitis due to listeria monocytogenes.spontaneous bacterial peritonitis (sbp) is a frequent and potentially deadly complication of ascites in patients with end-stage liver disease. unlike other pathogens more commonly implicated in sbp, listeria monocytogenes is a nonenteric organism that maybe acquired either sporadically or in the setting of foodborne outbreaks. listeria is an unusual cause of sbp that presents particular management challenges because of the organism's intrinsic resistance to first-line and empiric sbp treatment t ...201525960571
cheese-related listeriosis outbreak, portugal, march 2009 to february 2012.in portugal, listeriosis has been notifiable since april 2014, but there is no active surveillance programme for the disease. a retrospective study involving 25 national hospitals led to the detection of an outbreak that occurred between march 2009 and february 2012. the amount of time between the start of the outbreak and its detection was 16 months. of the 30 cases of listeriosis reported, 27 were in the lisbon and vale do tejo region. two cases were maternal/neonatal infections and one result ...201525955775
comparative efficacy of potassium levulinate with and without potassium diacetate and potassium propionate versus potassium lactate and sodium diacetate for control of listeria monocytogenes on commercially prepared uncured turkey breast.we evaluated the efficacy of potassium levulinate (klev; 0.0, 1.0, 1.5, and 2.0%) with and without a blend of potassium propionate (0.1%) and potassium diacetate (0.1%) (kpd) versus a blend of potassium lactate (1.8%) and sodium diacetate (0.125%) (kld) for inhibiting listeria monocytogenes on commercially prepared, uncured turkey breast during refrigerated storage. product formulated with kld or klev (1.5%) was also subsequently surface treated with 44 ppm of a solution of lauric arginate (lae) ...201525951386
development of predictive models for the growth kinetics of listeria monocytogenes on fresh pork under different storage temperatures.this study was conducted to develop a predictive model to estimate the growth of listeria monocytogenes on fresh pork during storage at constant temperatures (5, 10, 15, 20, 25, 30, and 35°c). the baranyi model was fitted to growth data (log cfu per gram) to calculate the specific growth rate (sgr) and lag time (lt) with a high coefficient of determination (r(2) > 0.98). as expected, sgr increased with a decline in lt with rising temperatures in all samples. secondary models were then developed ...201525951385
crucial roles of tnfaip8 protein in regulating apoptosis and listeria infection.tnf-α-induced protein 8 (tnfaip8 or tipe) is a newly described regulator of cancer and infection. however, its precise roles and mechanisms of actions are not well understood. we report in this article that tnfaip8 regulates listeria monocytogenes infection by controlling pathogen invasion and host cell apoptosis in a rac1 gtpase-dependent manner. tnfaip8-knockout mice were resistant to lethal l. monocytogenes infection and had reduced bacterial load in the liver and spleen. tnfaip8 knockdown in ...201525948813
centrifugal step emulsification applied for absolute quantification of nucleic acids by digital droplet rpa.aqueous microdroplets provide miniaturized reaction compartments for numerous chemical, biochemical or pharmaceutical applications. we introduce centrifugal step emulsification for the fast and easy production of monodisperse droplets. homogenous droplets with pre-selectable diameters in a range from 120 μm to 170 μm were generated with coefficients of variation of 2-4% and zero run-in time or dead volume. the droplet diameter depends on the nozzle geometry (depth, width, and step size) and inte ...201525947077
combined effect of thermosonication and slightly acidic electrolyzed water to reduce foodborne pathogens and spoilage microorganisms on fresh-cut kale.this study evaluated the efficacy of individual treatments (thermosonication [ts+dw] and slightly acidic electrolyzed water [sacew]) and their combination on reducing escherichia coli o157:h7, listeria monocytogenes, and spoilage microorganisms (total bacterial counts [tbc], enterobacteriaceae, pseudomonas spp., and yeast and mold counts [ymc]) on fresh-cut kale. for comparison, the antimicrobial efficacies of sodium chlorite (sc; 100 mg/l) and sodium hypochlorite (sh; 100 mg/l) were also evalua ...201525944413
improved recovery of listeria monocytogenes from stainless steel and polytetrafluoroethylene surfaces using air/water ablation.to develop a gentle ablation technique to recover listeria monocytogenes biofilms from stainless steel (ss) and polytetrafluoroethylene (ptfe) surfaces by using compressed air and water injection.201525943582
prevalence of listeria spp. and molecular characterization of listeria monocytogenes isolates from broilers at the abattoir.products from three broiler abattoirs were sampled for listeria species to evaluate the changes in the prevalence and contamination rates at two stages of processing. sampling was performed at the evisceration stage and at the end of processing after packaging and refrigerating at 4°c for 24 h. a total of 212 samples were collected; 52 were from abattoir a, and 80 samples each were collected from abattoirs b and c. among all samples, 99 (46.7%) tested positive for listeria, including l. monocyto ...201525942617
a novel mutation leading to a premature stop codon in inla of listeria monocytogenes isolated from neonatal listeriosis.the study objective was to investigate whether the strain of l. monocytogenes serotype 1/2c isolated from neonatal listeriosis carries a premature stop codon (pmsc) mutation in the inla gene. the strain was characterized by serotyping, macrorestriction analysis after digestion with the restriction enzyme asci, and sequencing of the inla gene. the tested strain of serotype 1/2c and pulsotype 1 possesses a new type of point mutation leading to a pmsc in the inla gene and production of truncated in ...201525938757
tlr2 and ap-1/nf-kappab are involved in the regulation of mmp-9 elicited by heat killed listeria monocytogenes in human monocytic thp-1 cells.mmp-9 is crucial for a normal immune response, but excessive release of this enzyme leads to severe tissue damage. listeria monocytogenes (lm) is an opportunistic food-borne pathogen causing listerosis, meningitis and sepsis. heat killed listeria monocytogenes (hklm) activates immune system and leads production of cytokines and chemokines. however, nothing is known about the involvement of hklm in mmp-9 regulation. therefore we investigated the role of hklm in the regulation of mmp-9 gene expres ...201525931987
outbreak of encephalitic listeriosis in red-legged partridges (alectoris rufa).an outbreak of neurological disease was investigated in red-legged partridges between 8 and 28 days of age. clinical signs included torticollis, head tilt and incoordination and over an initial eight day period approximately 30-40 fatalities occurred per day. no significant gross post mortem findings were detected. histopathological examination of the brain and bacterial cultures followed by partial sequencing confirmed a diagnosis of encephalitis due to listeria monocytogenes. further isolates ...201525921827
controlling listeria monocytogenes in cold smoked salmon with the antimicrobial peptide salmine.listeria monocytogenes (lm) is a major safety concern for smoked salmon producers, as it can survive both the brining and smoking process in cold smoked salmon production. salmine is a cationic antimicrobial peptide derived from the milt of salmon that has been shown to inhibit the growth of lm in vitro. commercialization of this peptide would add value to a waste product produced when raising salmon. the purpose of this study was to determine the anti-listeria activity of salmine in smoked salm ...201525920744
listeriosis outbreaks in british columbia, canada, caused by soft ripened cheese contaminated from environmental sources.soft ripened cheese (src) caused over 130 foodborne illnesses in british columbia (bc), canada, during two separate listeriosis outbreaks. multiple agencies investigated the events that lead to cheese contamination with listeria monocytogenes (l.m.), an environmentally ubiquitous foodborne pathogen. in both outbreaks pasteurized milk and the pasteurization process were ruled out as sources of contamination. in outbreak a, environmental transmission of l.m. likely occurred from farm animals to pe ...201525918702
three novel clade b serine protease inhibitors from disk abalone, haliotis discus discus: molecular perspectives and responses to immune challenges and tissue injury.serine protease inhibitors (serpins) control cellular protease activity in order to maintain cellular homeostasis. the immune and inflammatory responses of invertebrate clade b serpins have not been widely reported. in the present study, three proteins with high similarity to clade b serpins, referred to as abserpin-1, abserpin-2 and abserpin-3, were identified from disk abalone (haliotis discus discus). while abserpin-1 (399 aa) was of a typical size for this protein class, abserpin-2 (506 aa) ...201525917971
short communication: latin-style fresh cheese enhances lactic acid bacteria survival but not listeria monocytogenes resistance under in vitro simulated gastrointestinal conditions.different studies in humans have provided evidence about the health benefits of probiotics. however, most probiotic strains do not maintain good viability in the harsh conditions of the gastrointestinal tract (git). in the present study, latin-style fresh cheese produced with potential probiotic bacteria was tested to evaluate this cheese type as a food carrier for the delivery of viable microorganisms after exposure to simulated git conditions. the resistance of 28 lactic acid bacteria (lab) st ...201525912867
geographical and longitudinal analysis of listeria monocytogenes genetic diversity reveals its correlation with virulence and unique evolution.listeria monocytogenes is one of the most important foodborne pathogens causing severe diseases with a mortality rate of 24%. however, the genetic diversity and evolution of l. monocytogenes, particularly at the worldwide level, are poorly defined. in this study, we performed multilocus sequence typing (mlst) and multi virulence locus sequence typing (mvlst) for 86 l. monocytogenes strains derived from 8 countries from 1926 to 2012 in order to better understand the molecular evolution and geneti ...201525912377
virr-mediated resistance of listeria monocytogenes against food antimicrobials and cross-protection induced by exposure to organic acid salts.formulations of ready-to-eat (rte) foods with antimicrobial compounds constitute an important safety measure against foodborne pathogens such as listeria monocytogenes. while the efficacy of many commercially available antimicrobial compounds has been demonstrated in a variety of foods, the current understanding of the resistance mechanisms employed by l. monocytogenes to counteract these stresses is limited. in this study, we screened in-frame deletion mutants of two-component system response r ...201525911485
gene expression in listeria monocytogenes exposed to sublethal concentration of benzalkonium chloride.in this study, tolerance at sublethal concentration of benzalkonium chloride and transcription levels of mdrl, ladr, lde, sigb and bcrabc genes in listeria monocytogenes strains were evaluated. viable cells reduction occurred in 45% of strains and clinical isolates showed lower sensitivity than isolates from foods. an increased transcription of an efflux system encoding gene was found in 60% of strains, and simultaneous mdrl overexpression and ladr underexpression occurred in 30% of isolates. a ...201525910634
ultrasensitive detection and rapid identification of multiple foodborne pathogens with the naked eyes.in this study, a novel approach for ultrasensitive detection and rapid high-throughput identification of a panel of common foodborne pathogens with the naked eyes is presented. as a proof-of-concept application, a multiple pathogen analysis array is fabricated through immobilizing three specific polyt-capture probes which can respectively recognize rfbe gene (escherichia coli o157:h7), inva gene (salmonella enterica), inla gene (listeria monocytogenes) on the plastic substrates. pcr has been dev ...201525909338
molecular serotyping and pathogenic potential of listeria monocytogenes isolated from milk and milk products in tamil nadu, india.listeria monocytogenes, an important bacterial pathogen, is responsible for foodborne illnesses worldwide. examination of food samples for the presence of l. monocytogenes and assessment of their pathogenicity is usually an effective strategy in the prevention of listeriosis. in the present study, we have tested 307 samples of milk and milk products from various places in tamil nadu, india for the presence of l. monocytogenes using iso 11290 and u.s. food and drug administration bacteriological ...201525793931
complete genome sequence of the persistent listeria monocytogenes strain r479a.the complete genome sequence of the persistent listeria monocytogenes strain r479a isolated from smoked salmon in denmark and belonging to lineage ii, serovar 1/2a, and multilocus sequence type 8 (st8) is presented here.201525792065
sensitive enumeration of listeria monocytogenes and other listeria species in various naturally contaminated matrices using a membrane filtration method.for the enumeration of listeria monocytogenes (l. monocytogenes) in food, a sensitive enumeration method has been recently developed. this method is based on a membrane filtration of the food suspension followed by transfer of the filter on a selective medium to enumerate l. monocytogenes. an evaluation of this method was performed with several categories of foods naturally contaminated with l. monocytogenes. the results obtained with this technique were compared with those obtained from the mod ...201525791005
a novel gene, lstc, of listeria monocytogenes is implicated in high salt tolerance.listeria monocytogenes, causative agent of human listeriosis, has been isolated from a wide variety of foods including deli meats, soft cheeses, cantaloupes, sprouts and canned mushrooms. standard control measures for restricting microbial growth such as refrigeration and high salt are often inadequate as l. monocytogenes grows quite well in these environments. in an effort to better understand the genetic and physiological basis by which l. monocytogenes circumvents these controls, a transposon ...201525790994
enhancing the antibacterial effect of 461 and 521 nm light emitting diodes on selected foodborne pathogens in trypticase soy broth by acidic and alkaline ph conditions.light emitting diodes (leds) with their antibacterial effect present a novel method for food preservation. this effect may be influenced by environmental conditions such as the ph of the food contaminated by the pathogen. thus, it is necessary to investigate the influence of ph on the antibacterial effect of leds before their application to real food matrices. escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in trypticase soy broth were illuminated using 10-w 461 (22.1 ...201525790991
effects of oxygen-depleted atmospheres on survival and growth of listeria monocytogenes on fresh-cut iceberg lettuce stored at mild abuse commercial temperatures.the effects of oxygen-depleted atmospheres, 0.25% o2+12% co2 (balance n2) and 2% o2 + 6% co2 (balance n2), on growth of listeria monocytogenes on fresh-cut iceberg lettuce were determined. the study was carried out at mild abuse temperatures using controlled atmosphere chambers. during storage at a constant temperature of 7 °c, growth was enhanced at the lower oxygen level of 0.25% o2 by day 10. over 17 days of storage at temperatures designed to mimic mild abuse commercial conditions, there wer ...201525790986
notes from the field: listeriosis associated with stone fruit--united states, 2014.on july 19, 2014, a packing company in california (company a) voluntarily recalled certain lots of stone fruits, including whole peaches, nectarines, plums, and pluots, because of concern about contamination with listeria monocytogenes based on internal company testing. on july 31, the recall was expanded to cover all fruit packed at their facility during june 1-july 17. after the initial recall, clinicians, state and local health departments, cdc, and the food and drug administration (fda) rece ...201525789745
estimating the attack rate of pregnancy-associated listeriosis during a large outbreak.in 2011, a multistate outbreak of listeriosis linked to contaminated cantaloupes raised concerns that many pregnant women might have been exposed to listeria monocytogenes. listeriosis during pregnancy can cause fetal death, premature delivery, and neonatal sepsis and meningitis. little information is available to guide healthcare providers who care for asymptomatic pregnant women with suspected l. monocytogenes exposure.201525784782
a new bovine conjunctiva model shows that listeria monocytogenes invasion is associated with lysozyme resistance.listerial keratoconjunctivitis ('silage eye') is a wide spread problem in ruminants causing economic losses to farmers and impacts negatively on animal welfare. it results from direct entry of listeria monocytogenes into the eye, often following consumption of contaminated silage. an isolation protocol for bovine conjunctival swabbing was developed and used to sample both infected and healthy eyes bovine eyes (n=46). l. monocytogenes was only isolated from one healthy eye sample, and suggests th ...201525778543
stability of sublethal acid stress adaptation and induced cross protection against lauric arginate in listeria monocytogenes.the stability of acid stress adaptation in listeria monocytogenes and its induced cross protection effect against gras (generally recognized as safe) antimicrobial compounds has never been investigated before. in the present study, the acid stress adaptation in l. monocytogenes was initially induced in ph 5.0 tryptic soy broth supplemented with 0.6% yeast extract (tsb-ye) at 37 °c. subsequently, the stability of acid stress adaptation, which was defined as the capacity to maintain its acquired a ...201525777680
irf8 directs stress-induced autophagy in macrophages and promotes clearance of listeria monocytogenes.autophagy, activated by many stresses, plays a critical role in innate immune responses. here we show that interferon regulatory factor 8 (irf8) is required for the expression of autophagy-related genes in dendritic cells. furthermore in macrophages, irf8 is induced by multiple autophagy-inducing stresses, including ifnγ and toll-like receptor stimulation, bacterial infection, starvation and by macrophage colony-stimulating factor. irf8 directly activates many genes involved in various steps of ...201525775030
erratum to: lmo0171, a novel internalin-like protein, determines cell morphology of listeria monocytogenes and its ability to invade human cell lines. 201525773205
characterisation of the microflora contaminating the wooden vats used for traditional sicilian cheese production.traditional sicilian cheese productions are carried out employing traditional wooden vats, called tina. many studies have highlighted the beneficial role of wooden dairy equipment by contributing to enriching the milk microflora and improving the acidification processes. the present work was undertaken to evaluate the safety of the wooden vats used to coagulate milk. to this purpose, the different microbial populations hosted onto the internal surfaces of the vats used to produce two different s ...201527800376
tea and soybean extracts in combination with milk fermentation inhibit growth and enterocyte adherence of selected foodborne pathogens.this study examined the antibacterial and anti-adhesive properties of pure plant extracts (ppes) of green tea (gt), black tea (bt) and soybean individually or in combination with milk. fermented phenolic enriched-milk (fpem) was prepared by combining ppes with milk and fermented with lactic acid bacteria. antimicrobial activity of extracts was evaluated by broth-dilution and agar diffusion assay. anti-adhesive property of extracts was evaluated in caco-2 cell model. results from antibacterial te ...201525766833
[the metabolism of the innate immunity cells in bacterial infections].metabolic activity of innate immunity cells infected by various doses of gram-negative (yersinia pseudotuberculosis, salmonella enteritidis) and gram-positive (staphylococcus aureus, listeria monocytogenes) bacteria has been investigated. using various animal models we found that during the initial period (up to 2 days) changes of infection in cellular responses depend on the type of the pathogen. in response to infection caused by gram-negative bacteria predominant neutrophil accumulation in th ...201525762604
a novel therapy for melanoma developed in mice: transformation of melanoma into dendritic cells with listeria monocytogenes.listeria monocytogenes is a gram-positive bacteria and human pathogen widely used in cancer immunotherapy because of its capacity to induce a specific cytotoxic t cell response in tumours. this bacterial pathogen strongly induces innate and specific immunity with the potential to overcome tumour induced tolerance and weak immunogenicity. here, we propose a listeria based vaccination for melanoma based in its tropism for these tumour cells and its ability to transform in vitro and in vivo melanom ...201525760947
gas chromatography-mass spectrometry characterisation of the anti-listeria components of garcinia kola seeds.adsorption chromatography was used to separate the bioactive constituents of the crude n-hexane extract of garcinia kola seeds. the silica gel 60 column fractions were eluted using the solvent combination of benzene: ethanol : ammonium hydroxide (bea) in the ratio combination of 36 : 4 : 0.4 v/v. the fractions were tested for anti-listeria activities by determining their mic50, mic90 or mic against 4 listeria isolates. the fractions were labelled bea1 to bea5 and 3 out of the 5 fractions eluted ...201525757343
presence of listeria monocytogenes in mediterranean-style dry fermented sausages.the morphological, physiological and epidemiological features of l. monocytogenes, together with the severity of human listeriosis infections, make l. monocytogenes of particular concern for manufacturers of cold-stored "ready to eat" (rte) foods. l. monocytogenes has been isolated from a wide variety of rte foods and is responsible for several outbreaks associated with the consumption of rte meat, poultry, dairy, fish and vegetable products. although l. monocytogenes is among the most frequentl ...201528231188
antibiotic resistance of listeria monocytogenes isolated from meat-processing environments, beef products, and clinical cases in brazil.the present study aimed to assess the antimicrobial resistance and the presence of virulence markers in 137 listeria monocytogenes isolates obtained from meat-processing environments, beef products, and clinical cases. all isolates were subject to molecular serogrouping and their antibiotic resistance profiles were assessed against 12 antimicrobials. in addition, isolates were subjected to detection of virulence marker genes (inla, inlc, inlj). the isolates were classified into serogroups 4b, 4d ...201525756759
cxcr3 blockade protects against listeria monocytogenes infection-induced fetal wastage.mammalian pregnancy requires protection against immunological rejection of the developing fetus bearing discordant paternal antigens. immune evasion in this developmental context entails silenced expression of chemoattractant proteins (chemokines), thereby preventing harmful immune cells from penetrating the maternal-fetal interface. here, we demonstrate that fetal wastage triggered by prenatal listeria monocytogenes infection is driven by placental recruitment of cxcl9-producing inflammatory ne ...201525751061
novel inhibitor discovery and the conformational analysis of inhibitors of listeriolysin o via protein-ligand modeling.increasing bacterial resistance to available antibiotics makes the discovery of novel efficacious antibacterial agents a priority. a previous report showed that listeriolysin o (llo) is a critical virulence factor and suggested that it is a target for developing anti-virulence drugs against listeria monocytogenes infections. in this study, we report the discovery of llo natural compound inhibitors with differential activity by using hemolysis assay. the mechanism of action of the inhibitors was ...201525749652
rapid identification and source-tracking of listeria monocytogenes using maldi-tof mass spectrometry.listeria monocytogenes is an important foodborne pathogen responsible for the sometimes fatal disease listeriosis. public health concerns and stringent regulations associated with the presence of this pathogen in food and food processing environments underline the need for rapid and reliable detection and subtyping techniques. in the current study, the application of matrix assisted laser desorption/ionisation-time-of-flight mass spectrometry (maldi-tof ms) as a single identification and source- ...201525747262
label-free impedimetric detection of listeria monocytogenes based on poly-5-carboxy indole modified ssdna probe.listeria monocytogenes is a life threatening pathogenic bacteria concerned with human health. the accurate and rapid detection of l. monocytogenes is required for preventing of listeriosis. in this study, dna sensing probe based on conducting polymer poly-5-carboxy indole (5c pin) was developed for the detection of l. monocytogenes hlya gene responsible for pathogenicity. the probe sequences (24 mer ssdna) were covalently immobilized on 5c pin via n-(3-dimethylaminopropyl)-n'-ethylcarbodiimidehy ...201525746904
microbiological contamination of dried and lyophilized garlic as a potential source of food spoilage.garlic is valued more for its flavoring and used in a wide variety of foods. in food technology, fresh garlic is not used, but instead its processed forms, most often dried and lyophilized, are utilized. the quality and safety of the final product largely depends on their microbiological quality. this research has provided information about effect of garlic fixation methods and provided information about effect of microbiological contamination of garlic used as a spice for quality of garlic mayo ...201525745260
genome sequences of five disinfectant-resistant listeria monocytogenes strains from two iberian pork-processing plants.we announce the draft genome sequences of five listeria monocytogenes strains from two iberian pork-processing plants located in spain that were distinguished by their resistance to benzalkonium chloride. these strains seem highly adapted to the meat-processing environment according to their persistence and transmission capabilities.201525745005
whole-genome sequence of listeria monocytogenes serovar 4b strain izsam_lm_hs2008, isolated from a human infection in italy.listeria monocytogenes is one of the most important foodborne pathogens. in this report, we present the complete and annotated genome of l. monocytogenes sequence type 06 (st06) serovar 4b strain izsam_lm_hs2008, isolated from an adult immunocompetent patient who developed the disease and died.201525744995
listeria monocytogenes meningitis in an immunocompromised patient.this report describes a case of meningitis caused by listeria monocytogenes in a stem cell transplant recipient on immunosuppressive therapy for cutaneous chronic graft-versus host disease. a 59-year-old woman had undergone allogeneic stem cell transplantation (from a matched unrelated donor) 13 months previously for chronic lymphocytic leukemia. she was on regular hematologic follow-up. though her previous malignancy has been in remission, she was immunosuppressed due to the pharmacological tre ...201525742155
effect of chitosan on spoilage bacteria, escherichia coli and listeria monocytogenes in cured chicken meat.the effects of essential oil (eo) of oregano and chitosan on the microbial quality and growth inhibition of listeria monocytogenes and escherichia coli o157:h7 in the iranian traditional ready-to-barbecue chicken was evaluated. thus, three groups of samples were prepared. one of them was considered to evaluate for aerobic plate count (apc), lactic acid bacteria (lab), psychrophilic and enterobacteriacae counts and the other two groups were inoculated with e. coli o157:h7 and l. monocytogenes 4b ...201525735728
molecular cloning and characterization of autophagy-related gene tmatg8 in listeria-invaded hemocytes of tenebrio molitor.macroautophagy (hereinafter called autophagy) is a highly regulated process used by eukaryotic cells to digest portions of the cytoplasm that remodels and recycles nutrients and disposes of unwanted cytoplasmic constituents. currently 36 autophagy-related genes (atg) and their homologs have been characterized in yeast and higher eukaryotes, including insects. in the present study, we identified and functionally characterized the immune function of an atg8 homolog in a coleopteran insect, tenebri ...201525727880
outbreak investigation identifies a single listeria monocytogenes strain in sheep with different clinical manifestations, soil and water.listeria (l.) monocytogenes causes orally acquired infections and is of major importance in ruminants. little is known about l. monocytogenes transmission between farm environment and ruminants. in order to determine potential sources of infection, we investigated the distribution of l. monocytogenes genetic subtypes in a sheep farm during a listeriosis outbreak by applying four subtyping methods (maldi-tof-ms, mlst, mlva and pfge). l. monocytogenes was isolated from a lamb with septicemia and f ...201525726302
comparative proteomic analysis of listeria monocytogenes atcc 7644 exposed to a sublethal concentration of nisin.listeria monocytogenes infections have been frequently reported in many food poisoning outbreaks around the world. in this work, the protein repertoires of l. monocytogenes atcc 7644 cells treated or not with a 10(-3)mg/ml nisin sublethal concentration, established by antimicrobial susceptibility tests, were analyzed by lc-ms/ms. overall, 179 proteins were identified, 9 of them more abundant in nisin-treated samples, and 4 more abundant in non-treated control samples. in nisin treated cells, pro ...201525724729
previous physicochemical stress exposures influence subsequent resistance of escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes to ultraviolet-c in coconut liquid endosperm beverage.this study investigated the influences of prior exposures to common physicochemical stresses encountered by microorganisms in food and food processing ecologies such as acidity, desiccation, and their combinations, on their subsequent susceptibility towards uv-c treatment in coconut liquid endosperm beverage. cocktails of escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes were separately subjected to gradually acidifying environment (final ph 4.46), exposed to abrupt desic ...201525723813
plasmid-borne cadmium resistant determinants are associated with the susceptibility of listeria monocytogenes to bacteriophage.listeria monocytogenes is an intracellular pathogen causing gastroenteritis, central nervous system infections and abortions. chromosomal virulence determinants have been extensively investigated. however, the function of genes encoded by plasmids in l. monocytogenes has not been fully understood. in this study, we determined the prevalence and molecular profile of plasmids in food isolates of l. monocytogenes and examined the contribution of four plasmid-borne cadmium-resistant genes to the sus ...201525721472
whole-genome sequences of 12 clinical strains of listeria monocytogenes.listeria monocytogenes is a foodborne pathogen of global concern due to the high mortality rate among immunocompromised patients. whole-genome sequences of 12 strains of l. monocytogenes from humans were reported. the availability of these genomes should provide useful information on the evolutionary history and genetic diversity of l. monocytogenes.201525720683
seek and destroy process: listeria monocytogenes process controls in the ready-to-eat meat and poultry industry.the majority of human listeriosis cases appear to be caused by consumption of ready-to-eat (rte) foods contaminated at the time of consumption with high levels of listeria monocytogenes. although strategies to prevent growth of l. monocytogenes in rte products are critical for reducing the incidence of human listeriosis, control of postprocessing environmental contamination of rte meat and poultry products is an essential component of a comprehensive l. monocytogenes intervention and control pro ...201525710164
assessing the survival of listeria monocytogenes in a domestic freezer by analyzing subsequent growth at 30°c using a novel reference method.listeria monocytogenes is a serious pathogen capable of extensive survival under frozen storage. using optical density and multiple initial inocula in multiple identically prepared microtiter plates, the effect of storage time at -22°c on the subsequent growth at 30°c of the organism when defrosted was studied using a technique that compared the growth (through time to detection) of a test plate (previously frozen) with that of an identically prepared control plate, analyzed at the start of the ...201525710150
survival of salmonella, escherichia coli o157:h7, and listeria monocytogenes on raw peanut and pecan kernels stored at -24, 4, and 22°c.cocktails of lawn-collected cells were used to determine the survival of salmonella, escherichia coli o157:h7, and listeria monocytogenes on the surface of raw peanut and pecan kernels. kernels were inoculated with mixtures of four to five strains at 3 or 6 log cfu/g, dried at room temperature, and then stored at -24 ± 1, 4 ± 2, and 22 ± 1°c for 28 or 365 days. in most cases, rates of decline of the pathogens did not differ significantly between the two inoculum concentrations in the 28-day stud ...201525710147
estimation of listeria monocytogenes and escherichia coli o157:h7 prevalence and levels in naturally contaminated rocket and cucumber samples by deterministic and stochastic approaches.the aims of the present study were to determine the prevalence and levels of listeria monocytogenes and escherichia coli o157:h7 in rocket and cucumber samples by deterministic (estimation of a single value) and stochastic (estimation of a range of values) approaches. in parallel, the chromogenic media commonly used for the recovery of these microorganisms were evaluated and compared, and the efficiency of an enzyme-linked immunosorbent assay (elisa)-based protocol was validated. l. monocytogene ...201525710146
manure source and age affect survival of zoonotic pathogens during aerobic composting at sublethal temperatures.heat is the primary mechanism by which aerobic composting inactivates zoonotic bacterial pathogens residing within animal manures, but at sublethal temperatures, the time necessary to hold the compost materials to ensure pathogen inactivation is uncertain. to determine the influence of the type of nitrogen amendment on inactivation of salmonella, listeria monocytogenes, and escherichia coli o157:h7 in compost mixtures stored at sublethal temperatures, specific variables investigated in these stu ...201525710145
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