Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| antimicrobial susceptibility and antibiotic resistance gene transfer analysis of foodborne, clinical, and environmental listeria spp. isolates including listeria monocytogenes. | the aims of this study were to assess antibiotic resistance pheno- and genotypes in foodborne, clinical, and environmental listeria isolates, as well as to elucidate the horizontal gene transfer potential of detected resistance genes. a small fraction of in total 524 listeria spp. isolates (3.1%) displayed acquired antibiotic resistance mainly to tetracycline (n = 11), but also to clindamycin (n = 4) and trimethoprim (n = 3), which was genotypically confirmed. in two cases, a tetracycline resist ... | 2014 | 24382842 |
| enterococcus faecalis reconfigures its transcriptional regulatory network activation at different copper levels. | a global transcriptional regulatory network was generated in the pathogenic bacterium enterococcus faecalis in order to understand how this organism can activate and coordinate its expression at different copper concentrations. the topological evaluation of the network showed common patterns described in other organisms. integrating microarray experiments allowed the identification of two sub-networks activated at low (0.05 mm cuso4) and high (0.5 mm cuso4) concentrations of copper. the analysis ... | 2014 | 24382465 |
| presence and genetic environment of pleuromutilin-lincosamide-streptogramin a resistance gene lsa(e) in enterococci of human and swine origin. | 2014 | 24379302 | |
| effect of nanosilver gel, chlorhexidine gluconate, and camphorated phenol on enterococcus faecalis biofilm. | aim. to assess the effectiveness of nanosilver gel (nsg) in comparison to chlorhexidine gluconate (chx) and camphorated phenol (cp) against enterococcus faecalis (e.f) biofilm. methods and materials. two tests were done, methyl thiazolyl tetrazolium (mtt) assay and confocal laser scanning microscopy (clsm) analysis, to determine the effectiveness of nsg, chx, and cp on e.f biofilm. polystyrene microtiter 96- and 6-well plates were used for mtt and clsm, respectively. nanosilver gel was in three ... | 2014 | 27355067 |
| study of the antibacterial efficacy of bainiku-ekisu against pathogens. | the research was undertaken to determine the bacteriostatic effects of the concentrate of japanese apricot juice (bainiku-ekisu), which is a popular health food in taiwan and japan, on enterococcus faecalis atcc 29212, staphylococcus aureus atcc 25923, and escherichia coli atcc 25922. the results show that e. faecalis, s. aureus, and e. coli could be killed or inhibited by bainiku-ekisu at concentrations between 1.0 and 10.0 mg/ml. the minimum inhibitory concentration (mic) was 1 mg/ml for all s ... | 2014 | 26904733 |
| seasonal variations in bacterial communities and antibiotic-resistant strains associated with green bottle flies (diptera: calliphoridae). | green bottle flies occur frequently around human environments in japan. many species of green bottle flies have been studied with regard to their importance in forensic examinations or clinical therapies, but the bacterial communities associated with this group of flies have not been comprehensively investigated. in this research, 454 pyrosequencing was used to reveal the bacterial communities in green bottle flies collected in different seasons. meanwhile, the bacteria were screened with select ... | 2014 | 24413921 |
| ocular penetration and efficacy of levofloxacin using different drug-delivery techniques for the prevention of endophthalmitis in rabbit eyes with posterior capsule rupture. | to evaluate the effects of different drug-delivery techniques for levofloxacin (lvfx) in ocular penetration and the prevention of endophthalmitis using an aphakic rabbit model with posterior capsule rupture (pcr). | 2014 | 24410272 |
| chitosan-cellulose composite for wound dressing material. part 2. antimicrobial activity, blood absorption ability, and biocompatibility. | chitosan (cs), a polysaccharide derived from chitin, the second most abundant polysaccharide, is widely used in the medical world because of its natural and nontoxic properties and its innate ability for antibacterial and hemostasis effects. in this study, the novel composites containing cs and cellulose (cel) (i.e., [cel + cs]), which we have previously synthesized using a green and totally recyclable method, were investigated for their antimicrobial activity, absorption of anticoagulated whole ... | 2014 | 24407857 |
| impact of actin on adhesion and translocation of enterococcus faecalis. | this study focuses on the impact of actin on adhesion and translocation of enterococcus (e.) faecalis og1rf, e. faecalis symbioflor(®), and e. faecalis v583. insight into the role of actin aggregation in the mediation of bacterial adhesion and translocation was provided by a two-chamber translocation assay, which employed ptk6 cells. determination of translocation rates, cytochalasin d treatment, and laser scanning confocal microscopic observation revealed actin as a predominant brace for entero ... | 2014 | 24362949 |
| residual activity of cetrimide and chlorhexidine on enterococcus faecalis-infected root canals. | effective final irrigation regimen is an important step in order to achieve better disinfection and ensure residual antimicrobial effects after root canal preparation. the aim of this study was to compare the residual antimicrobial activity of 0.2% cetrimide, and 0.2% and 2% chlorhexidine in root canals infected with enterococcus faecalis. biofilms of e. faecalis were grown on uniradicular roots for 4 weeks. after root canal preparation, root canals were irrigated with 17% ethylenediaminetetraac ... | 2014 | 24357857 |
| co-transfer of resistance to high concentrations of copper and first-line antibiotics among enterococcus from different origins (humans, animals, the environment and foods) and clonal lineages. | we studied the occurrence of diverse copper (cu) tolerance genes from gram-positive bacteria and their co-transfer with antibiotic resistance genes among enterococcus from diverse sources. | 2014 | 24343895 |
| role of house flies in the ecology of enterococcus faecalis from wastewater treatment facilities. | enterococci are important nosocomial pathogens, with enterococcus faecalis most commonly responsible for human infections. in this study, we used several measures to test the hypothesis that house flies, musca domestica (l.), acquire and disseminate antibiotic-resistant and potentially virulent e. faecalis from wastewater treatment facilities (wwtf) to the surrounding urban environment. house flies and sludge from four wwtf (1-4) as well as house flies from three urban sites close to wwtf-1 were ... | 2014 | 24337146 |
| in vitro regulation of ccl3 and cxcl12 by bacterial by-products is dependent on site of origin of human oral fibroblasts. | production of chemokines by tissue resident cells is one of the main mechanisms involved in the inflammatory infiltrate formation during inflammation. the specific ability of fibroblasts from different oral tissues such as gingiva, periodontal ligament, and dental pulp from permanent and deciduous teeth in producing the chemokines ccl3 and cxcl12 under stimulation by bacterial products commonly found in endodontic infections was investigated. | 2014 | 24331998 |
| impact of enterococcal colonization and infection in solid organ transplantation recipients from the swiss transplant cohort study. | the burden of enterococcal infections has increased over the last decades with vancomycin-resistant enterococci (vre) being a major health problem. solid organ transplantation is considered as a risk factor. however, little is known about the relevance of enterococci in solid organ transplantation recipients in areas with a low vre prevalence. | 2014 | 24330137 |
| antibiotic resistance in enterococcus faecium clinical isolates. | the worldwide ratio of enterococcus faecalis-enterococcus faecium infections is currently changing in favor of e. faecium. intrinsic and acquired antimicrobial resistance traits of this latter species can explain this evolution as well as the diffusion of hospital-adapted strains belonging to the clonal complex cc17. like other enterococci, e. faecium is naturally resistant to cephalosporins and aminoglycosides (at low level). because of its high genome plasticity, it can also acquire numerous o ... | 2014 | 24392717 |
| molecular epidemiology of enterococcal bacteremia in australia. | enterococci are a major cause of health care-associated infections and account for approximately 10% of all bacteremias globally. the aim of this study was to determine the proportion of enterococcal bacteremia isolates in australia that are antimicrobial resistant, with particular emphasis on susceptibility to ampicillin and the glycopeptides, and to characterize the molecular epidemiology of the enterococcus faecalis and enterococcus faecium isolates. from 1 january to 31 december 2011, 1,079 ... | 2014 | 24391201 |
| genetic basis for vancomycin-enhanced cephalosporin susceptibility in vancomycin-resistant enterococci revealed using counterselection with dominant-negative thymidylate synthase. | antibiotic-resistant enterococci are major causes of hospital-acquired infections. all enterococci are intrinsically resistant to most cephalosporins, antibiotics in the beta-lactam family that impair peptidoglycan synthesis by inactivating the transpeptidases responsible for cross-linking. in addition, clinical isolates of enterococci often possess acquired resistance to vancomycin, a glycopeptide antibiotic that impairs peptidoglycan biosynthesis by a mechanism distinct from that of the beta-l ... | 2014 | 24366749 |
| pathways and roles of wall teichoic acid glycosylation in staphylococcus aureus. | the thick peptidoglycan layers of gram-positive bacteria are connected to polyanionic glycopolymers called wall teichoic acids (wta). pathogens such as staphylococcus aureus, listeria monocytogenes, or enterococcus faecalis produce wta with diverse, usually strain-specific structure. extensive studies on s. aureus wta mutants revealed important functions of wta in cell division, growth, morphogenesis, resistance to antimicrobials, and interaction with host or phages. while most of the s. aureus ... | 2014 | 24365646 |
| antimicrobial efficacy of photodynamic therapy and sodium hypochlorite on monoculture biofilms of enterococcus faecalis at different stages of development. | as the enterococcus faecalis biofilm ages, it is likely that bacteria in mature and old biofilms will become more resistant to commonly used antibacterial irrigants. photodynamic therapy (pdt) has been used to kill resistant bacteria organized in a biofilm. this study aimed to investigate the antimicrobial effectiveness of 1% sodium hypochlorite (naocl), 2.5% naocl, and pdt on e. faecalis biofilm at different stages of development. | 2014 | 24308707 |
| comparison of peptide nucleic acid fluorescence in situ hybridization assays with culture-based matrix-assisted laser desorption/ionization-time of flight mass spectrometry for the identification of bacteria and yeasts from blood cultures and cerebrospinal fluid cultures. | peptide nucleic acid fluorescence in situ hybridization (pna fish) is a molecular diagnostic tool for the rapid detection of pathogens directly from liquid media. the aim of this study was to prospectively evaluate pna fish assays in comparison with culture-based matrix-assisted laser desorption/ionization-time of flight mass spectrometry (maldi-tof ms) identification, as a reference method, for both blood and cerebrospinal fluid (csf) cultures, during a 1-year investigation. on the basis of the ... | 2014 | 24304149 |
| in vitro activity of amazon plant extracts against enterococcus faecalis. | previous studies analyzing 2,200 plant extracts indicated anti-enterococcal activity in 25 extracts obtained from brazilian forests' plants. in the present study, these extracts were subjected to microdilution broth assay (mdba) and disk diffusion assay (dda) using planktonic enterococcus faecalis atcc(®) 29212™ and were submitted to phytochemical analysis in tlc and hplc. three extracts obtained from ipomoea alba (mic < 40 μg/ml), diclinanona calycina (mic ≤ 40 μg/ml) and moronobea coccinea (40 ... | 2014 | 25477906 |
| community-acquired methicillin-resistant staphylococcus epidermidis pyelonephritis in a child: a case report. | staphylococcus epidermidis is currently the most frequent pathogen of opportunistic and nosocomial infections worldwide. most cases of staphylococcus epidermidis infections are associated with indwelling medical devices and/or immunocompromised conditions. community-acquired urinary tract infections are rare, particularly among pediatric populations, and clinicians often do not consider staphylococcus epidermidis as a uropathogen. | 2014 | 25488491 |
| efficacy of antimicrobial photodynamic therapy for root canals infected with enterococcus faecalis. | effective decontamination of root canal systems is a constant concern in clinical practice. in this article, we compare the performance of photodynamic therapy (pdt) and sodium hypochlorite (naocl) in reducing the amount of enterococcus faecalis in root canals. relevant studies were identified by searching electronic databases, including web of science, pubmed, bvs (medline, scielo, lilacs and bbo), scopus, and cochrane, and by manually searching the references of identified studies. the terms u ... | 2014 | 25500925 |
| in-vitro screening of malaysian honey from different floral sources for antibacterial activity on human pathogenic bacteria. | different researches on therapeutic effects of honey have been conducted in different regions; however the study on the potential antibacterial activity of malaysian honey is still limited. in this study, antibacterial activities of different monofloral honey samples were tested against several common human pathogenic bacteria. | 2014 | 25435614 |
| assessment of the in vitro synergy of daptomycin plus linezolid against multidrug-resistant enterococci. | the widespread incidence of enterococci resistant to ampicillin, vancomycin and aminoglycosides, the first-line anti-enterococcal antibiotics, has made the treatment of severe enterococcal infections difficult and alternatives should be explored. we investigated the activity of daptomycin combined with linezolid against three enterococcus faecalis and four enterococcus faecium strains resistant to standard drugs used for therapy. minimum inhibitory concentrations (mics) were determined by the br ... | 2014 | 27873692 |
| vancomycin-resistant enterococci: troublemaker of the 21st century. | the emergence of multidrug-resistant and vancomycin-resistant enterococci during the last decade has made it difficult to treat nosocomial infections. although various enterococcal species have been identified, only two (enterococcus faecalis and enterococcus faecium) are responsible for the majority of human infections. vancomycin is an important therapeutic alternative against multidrug-resistant enterococci but is associated with a poor prognosis. resistance to vancomycin dramatically reduces ... | 2014 | 27873678 |
| effect of zirconium oxide and zinc oxide nanoparticles on physicochemical properties and antibiofilm activity of a calcium silicate-based material. | the aim of the present study was to evaluate the antibiofilm activity against enterococcus faecalis, compressive strength. and radiopacity of portland cement (pc) added to zirconium oxide (zro2), as radiopacifier, with or without nanoparticulated zinc oxide (zno). the following experimental materials were evaluated: pc, pc + zro2, pc + zro2 + zno (5%), and pc + zro2 + zno (10%). antibiofilm activity was analyzed by using direct contact test (dct) on enterococcus faecalis biofilm, for 5 h or 15 h ... | 2014 | 25431798 |
| penicillin-resistant, ampicillin-susceptible enterococcus faecalis of hospital origin: pbp4 gene polymorphism and genetic diversity. | despite the spread of penicillin-resistant, ampicillin-susceptible enterococcus faecalis (prasef) isolates in diverse countries, the mechanisms leading to this unusual resistance phenotype have not yet been investigated. the aim of this study was to evaluate whether polymorphism in the pbp4 gene is associated with penicillin resistance in prasef isolates and to determine their genetic diversity. e. faecalis isolates were recovered from different clinical specimens of hospitalized patients from f ... | 2014 | 25445645 |
| [enterococcal infections: from simple to most complex]. | enterococci are microorganisms with a remar- kable ability to adapt to their environment. two species have a significant clinical implication, enterococcus faecalis and enterococcus faecium. the risk factors for colonization and infection must be recognized, including prior treatment with antibiotics such as cephalosporins or quinolones. because of their native resistance to several classes of antibiotics and the increase of acquired resistance to penicillins, the initial empiric treatment of a ... | 2014 | 25438375 |
| identification of peptidoglycan-associated proteins as vaccine candidates for enterococcal infections. | infections by opportunistic bacteria have significant contributions to morbidity and mortality of hospitalized patients and also lead to high expenses in healthcare. in this setting, one of the major clinical problems is caused by gram-positive bacteria such as enterococci and staphylococci. in this study we extract, purify, identify and characterize immunogenic surface-exposed proteins present in the vancomycin resistant enterococci (vre) strain enterococcus faecium e155 using three different e ... | 2014 | 25369230 |
| antibacterial efficacy of aqueous ozone in root canals infected by enterococcus faecalis. | in endodontics, the elimination of resistant bacteria such as enterococcus faecalis plays an important role for treatment success in root canals. therefore, new alternative irrigants (instead of sodium hypochlorite) have been researched to achieve ideal endodontic treatment. | 2014 | 25368798 |
| anti-bacterial and anti-oxidant activities of leaf extracts of combretum vendae (combretecacea) and the isolation of an anti-bacterial compound. | combretum vendae a.e. van wyk (combretaceae) is used for the treatment of bacterial related infections and oxidative related diseases by indigenous people of south africa. dried leaves extracts of c. vendae were investigated for bioactivity against a variety of bacterial strains and their antioxidant potential evaluated. | 2014 | 25395708 |
| australian enterococcal sepsis outcome progamme, 2011. | from 1 january to 31 december 2011, 29 institutions around australia participated in the australian enterococcal sepsis outcome programme (aesop). the aim of aesop 2011 was to determine the proportion of enterococcal bacteraemia isolates in australia that are antimicrobial resistant, with particular emphasis on susceptibility to ampicillin and the glycopeptides, and to characterise the molecular epidemiology of the enterococcus faecalis and e. faecium isolates. of the 1,079 unique episodes of ba ... | 2014 | 25391408 |
| bacterial leakage in root canals filled with resin-based and mineral trioxide aggregate-based sealers. | sealing ability is one of the most important features of endodontic sealers. the purpose of this in vitro study was to compare the sealing ability of a resin-based sealer with a mineral trioxide aggregate-based sealer. | 2014 | 25426153 |
| comparative structural and functional analysis of staphylococcus aureus glucokinase with other bacterial glucokinases. | glucokinase is classified in bacteria based upon having atp binding site and 'repressor/open reading frames of unknown function/sugar kinases' motif, the sequence of glucokinase gene (jn645812) of staphylococcus aureus atcc12600 showed presence of atp binding site and 'repressor/open reading frames of unknown function/sugar kinases' motif. we have earlier observed glucokinase of s. aureus has higher affinity towards the substrate compared to other bacterial glucokinase and under anaerobic condit ... | 2014 | 25425757 |
| in vitro activity of heather [calluna vulgaris (l.) hull] extracts on selected urinary tract pathogens. | calluna vulgaris l. hull (ericaceae) has been used for treatment of urinary tract infections in traditional medicine. in this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of escherichia coli, enterococcus faecalis and proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) were determined. the concentrations of total phenols ... | 2014 | 25428676 |
| an agmatine-inducible system for the expression of recombinant proteins in enterococcus faecalis. | scientific interest in enterococcus faecalis has increased greatly over recent decades. some strains are involved in food fermentation and offer health benefits, whereas others are vancomycin-resistant and cause infections that are difficult to treat. the limited availability of vectors able to express cloned genes efficiently in e. faecalis has hindered biotechnological studies on the bacterium's regulatory and pathogenicity-related genes. the agmatine deiminase (agdi) pathway of e. faecalis, i ... | 2014 | 25471381 |
| blood stream infections in renal transplant recipients: a single-center study. | bacteremias among renal transplant recipients are more frequent as a result of immunosuppression. they are considered extremely high-risk because they are correlated with decreased allograft and recipient survival. | 2014 | 25420856 |
| mechanisms of linezolid resistance in staphylococci and enterococci isolated from two teaching hospitals in shanghai, china. | linezolid is one of the most effective treatments against gram-positive pathogens. however, linezolid-resistant/intermediate strains have recently emerged in worldwide. the purpose of this study was to analyse the prevalence and resistance mechanisms of linezolid-resistant/intermediate staphylococci and enterococci in shanghai, china. | 2014 | 25420718 |
| effects of adsorbed and templated nanosilver in mesoporous calcium-silicate nanoparticles on inhibition of bacteria colonization of dentin. | mesoporous calcium-silicate nanoparticles (mcsns) are advanced biomaterials for controlled drug delivery and mineralization induction. nanosilver-incorporated mcsns (ag-mcsns) were prepared in the present study using both the adsorption and template methods. both versions of ag-mcsns showed characteristic morphology of mesoporous materials and exhibited sustained release of ions over time. in antibacterial testing against planktonic enterococcus faecalis, ag-mcsns showed significantly better ant ... | 2014 | 25419127 |
| molecular characterization and antibiotic resistance of enterococcus species from gut microbiota of chilean altiplano camelids. | enterococcus is one of the major human pathogens able to acquire multiple antibiotic-resistant markers as well as virulence factors which also colonize remote ecosystems, including wild animals. in this work, we characterized the enterococcus population colonizing the gut of chilean altiplano camelids without foreign human contact. | 2014 | 25405007 |
| comparison of the antibacterial efficacy of manuka honey against e.faecalis and e.coli - an in vitro study. | to compare the antibacterial efficacy of manuka honey against e.faecalis and e.coli. | 2014 | 25302263 |
| a delicate balance: maintaining mutualism to prevent disease. | the intestinal microbial ecosystem is complex, and few of the principles that contribute to homeostasis in health are well understood. pham et al. (2014) show that a network including the epithelial interleukin-22 receptor protects against infection with the opportunistic pathogen enterococcus faecalis through promotion of host-microbiota mutualism. | 2014 | 25299326 |
| viability and antibacterial efficacy of four root canal disinfection techniques evaluated using confocal laser scanning microscopy. | several disinfection techniques have been recently introduced with the main objective of improving root canal disinfection in the inaccessible areas of the root canal system. this in vitro study was done to evaluate the antimicrobial effect and viability of enterococcus faecalis biofilms using conventional irrigation, endoactivator (dentsply, tulsa dental, usa), diode laser irradiation and photon-initiated photoacoustic streaming (pips). | 2014 | 25298645 |
| antibacterial effect of triantibiotic mixture versus calcium hydroxide in combination with active agents against enterococcus faecalis biofilm. | the aim of the present study was to compare the antibacterial effect of calcium hydroxide (ch), triantibiotic mixture (tam), and ch in combination with chlorhexidine (chx), sodium hypochlorite (naocl) or colchicine (col) against enterococcus faecalis (e. faecalis) in surface and deep dentinal tubules. seventy five fresh single-rooted human teeth were infected and divided into five experimental groups (n=15). the experimental groups were treated with ch+distilled water, ch+chx, ch+naocl, ch+col+d ... | 2014 | 25297855 |
| a novel role for d-alanylation of lipoteichoic acid of enterococcus faecalis in urinary tract infection. | enterococci are the third most common cause of healthcare-associated infections, which include urinary tract infections, bacteremia and endocarditis. cell-surface structures such as lipoteichoic acid (lta) have been poorly examined in e. faecalis, especially with respect to urinary tract infections (utis). the dlt operon is responsible for the d-alanylation of lta and includes the gene dlta, which encodes the d-alanyl carrier protein ligase (dcl). the involvement of lta in uti infection by e. fa ... | 2014 | 25296179 |
| in vitro activity of oritavancin against gram-positive pathogens isolated in canadian hospital laboratories from 2011 to 2013. | gram-positive pathogens isolated in 15 canadian hospital laboratories between 2011 and 2013 were tested for susceptibility to oritavancin and comparative antimicrobial agents using the clinical and laboratory standards institute broth microdilution method. oritavancin demonstrated in vitro activity equivalent to, or more potent than, vancomycin, daptomycin, linezolid, and tigecycline against the isolates of methicillin-susceptible staphylococcus aureus (n=1460; oritavancin mic90, 0.06 μg/ml; 99. ... | 2014 | 25294303 |
| large vegetation in a 60-year-old man with enterococcus faecalis cardiac implantable electronic device infection. | 2014 | 25293687 | |
| complete genome assembly of enterococcus faecalis 29212, a laboratory reference strain. | enterococcus faecalis is a nonmotile gram-positive coccus, found both as a commensal organism in healthy humans and animals and as a causative agent of multiple diseases, in particular endocarditis. we sequenced the genome of e. faecalis atcc 29212, a commonly used reference strain in laboratory studies, to complete "finished" annotated assembly (3 mb). | 2014 | 25291775 |
| infantile subdural empyema: the role of brain sonography and percutaneous subdural tapping in a resource-challenged region. | this study explored the outcome of children with patent anterior fontanelles who were treated with trans-fontanelle ultrasound scan (tfuss), which is more affordable and available than ct scan and mri in the diagnosis of childhood intracranial pathologies and treatment of subdural empyema, in developing countries. | 2014 | 25288836 |
| effects of storage temperature on tyramine production by enterococcus faecalis r612z1 in water-boiled salted ducks. | tyramine production by enterococcus faecalis r612z1 in water-boiled salted ducks was evaluated during storage at different temperatures. the results showed that e. faecalis r612z1 could produce tyramine in meat samples when the storage temperature was no less than 4°c. the e. faecalis r612z1 counts of the meat samples reached 10(8) cfu/g on day 7 at 4°c and on day 4 at 10°c. however, the tyramine content of the meat samples stored at 10°c increased to 23.73 μg/g (on day 10), which was greater th ... | 2014 | 25285502 |
| mapping of the modular closthioamide architecture reveals crucial motifs of polythioamide antibiotics. | closthioamide, the first known secondary metabolite from an anaerobic microorganism (clostridium cellulolyticum), represents a highly potent antibiotic that is active against methicillin-resistant staphylococcus aureus (mrsa) and vancomycin-resistant enterococcus faecalis (vre) at nanomolar concentrations. to unveil structure-activity relationships of the unusual polythioamide natural product we have designed a synthetic grid to access analogues with altered terminal aromatic moieties, diverse p ... | 2014 | 25284750 |
| a comparative evaluation of antibacterial efficacy of 'activ points' and 'combi points' as intra-canal medicaments against enterococcus faecalis: an ex vivo study. | enterococcus faecalis has been found to be one of the most predominant bacterial species associated with failed endodontic cases. the ability of this microbe to form biofilms; penetrate into dentinal tubules; survival in low ph, high salinity and high temperatures and resistance to many intracanal medicaments, makes it one of the most resistant pathogen of all the root canal flora. studies have shown that chlorhexidine is relatively more effective against e. faecalis as compared to other intra c ... | 2014 | 25284521 |
| new antibacterial xanthone from the marine sponge-derived micrococcus sp. eg45. | microluside a [4 (19-para-hydroxy benzoyloxy-o-β-d-cellobiosyl), 5 (30-para-hydroxy benzoyloxy-o-β-d-glucopyranosyl) xanthone (1)] is a unique o-glycosylated disubstituted xanthone isolated from the broth culture of micrococcus sp. eg45 cultivated from the red sea sponge spheciospongia vagabunda. the structure of microluside a was determined by 1d- and 2d-nmr techniques as well as high resolution tandem mass spectrometry. the antimicrobial activity evaluation showed that 1 exhibited antibacteria ... | 2014 | 25283555 |
| comparative evaluation of calcium hypochlorite and sodium hypochlorite associated with passive ultrasonic irrigation on antimicrobial activity of a root canal system infected with enterococcus faecalis: an in vitro study. | the purpose of this study was to compare in vitro the effectiveness of calcium hypochlorite (ca[ocl]2) and sodium hypochlorite (naocl) associated with passive ultrasonic irrigation in root canals of bovine teeth infected with enterococcus faecalis. | 2014 | 25282371 |
| biohybrid polymer-antimicrobial peptide medium against enterococcus faecalis. | antimicrobial peptides (amps) are conserved evolutionary components of the innate immune system that are being tested as alternatives to antibiotics. slow release of amps using biodegradable polymers can be advantageous in maintaining high peptide levels for topical treatment, especially in the oral environment in which dosage retention is challenged by drug dilution with saliva flow and by drug inactivation by salivary enzymatic activity. enterococcus faecalis is a multidrug resistant nosocomia ... | 2014 | 25279943 |
| evolution of the resistance to antibiotics of bacteria involved in urinary tract infections: a 7-year surveillance study. | we conducted a retrospective analysis on the identification and antibiogram of all bacteria isolated from urine samples with microbiological confirmation of urinary tract infection (uti) in a spanish reference hospital over a 7-year period. | 2014 | 25278389 |
| commensal bacteria directly suppress in vitro degranulation of mast cells in a myd88-independent manner. | the intestine harbors a substantial number of commensal bacteria that provide considerable benefits to the host. epidemiologic studies have identified associations between alterations in the composition of the intestinal microbiota and the development of allergic disease. however, the cellular and molecular mechanisms underlying these effects remain to be determined. here, we show that heat-killed commensal bacteria suppressed degranulation of mast cells in vitro in a myd88-independent manner. i ... | 2014 | 25273132 |
| healthcare-associated infective endocarditis: a case series in a referral hospital from 2006 to 2011. | healthcare-associated infective endocarditis (hca-ie), a severe complication of medical care, shows a growing incidence in literature. | 2014 | 25352503 |
| [study of association between parvimonas micra and pulp dominant pathogens in the infected root canals with chronic periradicular periodontitis]. | to study the prevalence of parvimonas micra (pm) and the associations between pm and pulp dominant pathogens in order to reflect the colonization of pm in the infected root canals with chronic periradicular periodontitis. | 2014 | 25351224 |
| effect of photodynamic therapy (pdt) on enterococcus faecalis biofilm in experimental primary and secondary endodontic infections. | to determine the antibacterial effect of photodynamic therapy on enterococcus faecalis (e. faecalis) biofilms in experimentally infected human root canals in primary infections and endodontic retreatments. | 2014 | 25366394 |
| comparative prevalence of antimicrobial resistance in community-acquired urinary tract infection cases from representative states of northern and southern india. | urinary tract infections (utis) are amongst the most common infections described in outpatient settings. increased antimicrobial resistance (amr) of urinary tract pathogens is a matter of global public health concern. treatment of uti depends on both prevalence and antimicrobial resistance (amr) of causative bacteria at any specific geographical location. | 2014 | 25386432 |
| an in vitro comparison of the antibacterial efficacy of triphala with different concentrations of sodium hypochlorite. | the antimicrobial efficacy of root canal irrigant plays an important role in increasing the success of root canal treatment (rct). the aim of the present experimental study was to compare the antimicrobial activity of triphala (a plant-derived solution) with 0.5, 1, 2.5 and 5% concentrations of sodium hypochlorite (naocl), against enterococcus faecalis (e. faecalis). | 2014 | 25386211 |
| central line associated blood stream infection rate after intervention and comparing outcome with national healthcare safety network and international nosocomial infection control consortium data. | benchmarking of central line associated blood stream infection (clabsi) rates remains a problem in developing countries due to the variations in surveillance practices and/or infection risk as non-availability of national data. | 2014 | 25328774 |
| riboflavin-shuttled extracellular electron transfer from enterococcus faecalis to electrodes in microbial fuel cells. | great attention has been focused on gram-negative bacteria in the application of microbial fuel cells. in this study, the gram-positive bacterium enterococcus faecalis was employed in microbial fuel cells. bacterial biofilms formed by e. faecalis zer6 were investigated with respect to electricity production through the riboflavin-shuttled extracellular electron transfer. trace riboflavin was shown to be essential for transferring electrons derived from the oxidation of glucose outside the peptid ... | 2014 | 25345758 |
| engineering acetyl coenzyme a supply: functional expression of a bacterial pyruvate dehydrogenase complex in the cytosol of saccharomyces cerevisiae. | the energetic (atp) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. cytosolic acetyl coenzyme a (acetyl-coa) is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into saccharomyces cerevisiae, such as isoprenoids or lipids. in this yeast, synthesis of cytosolic acetyl-coa via acetyl-coa synthetase (acs) involves hydrolysis of atp to amp and pyrophosphat ... | 2014 | 25336454 |
| pyranoside-into-furanoside rearrangement: new reaction in carbohydrate chemistry and its application in oligosaccharide synthesis. | great interest in natural furanoside-containing compounds has challenged the development of preparative methods for their synthesis. herein a novel reaction in carbohydrate chemistry, namely a pyranoside-into-furanoside (pif) rearrangement permitting the transformation of selectively o-substituted pyranosides into the corresponding furanosides is reported. the discovered process includes acid-promoted sulfation accompanied by rearrangement of the pyranoside ring into a furanoside ring followed b ... | 2014 | 25319316 |
| optimizing therapy for vancomycin-resistant enterococcal bacteremia in children. | uncertainties exist regarding the optimal treatment for vancomycin-resistant enterococcal (vre) bloodstream infections, particularly in settings in which ampicillin cannot be used. | 2014 | 25313503 |
| antimicrobial activity of extracts and isoquinoline alkaloids of selected papaveraceae plants. | alkaloidal extracts of seven selected plants of the family papaveraceae were studied with respect to their activity against six strains of pathogenic bacteria and their alkaloidal fingerprint. twenty-four alkaloids were determined by gc/ms, and twenty of them identified from their mass spectra, retention times and retention indexes. in the antibacterial assay, three gram-positive (enterorococcus faecalis, staphylococcus aureus and s. hyicus), and three gram-negative (escherichia coli, proteus mi ... | 2014 | 25632464 |
| efficacy of contemporary and novel intracanal medicaments against enterococcus faecalis. | to compare the antibacterial activity of propolis (30% in methyl cellulose), curcumin (2.5mg/ml of methyl cellulose), 2% chlorhexidine gel (chx), 2% metronidazole gel (mz) and a mixture of 2% chx and 2% mz against enterococcus faecalis in vitro. calcium hydroxide served as the control. | 2014 | 25631726 |
| an in vivo comparison of antimicrobial efficacy of sodium hypochlorite and biopure mtad™ against enterococcus faecalis in primary teeth: a qpcr study. | biopure mtad™, a new root canal irrigant has shown promising results against the most common resistant microorganism, e. faecalis, in permanent teeth. however, there is lack of studies comparing its antimicrobial effectiveness with naocl in primary teeth. the purpose of this study was to compare the in vivo antimicrobial efficacy of naocl 2.5% and biopure mtad™ against e. faecalis in primary teeth. | 2014 | 25631723 |
| vancomycin-resistant enteroccus faecium and enterococcus faecalis isolated from education hospital of iran. | enterococci are opportunistic pathogens which represent one of the leading agents of nosocomial infections, especially urinary tract infections (uti) in hospitalized patients. the aim of the present study was to determine the resistance pattern and the type of resistance genes in vancomycin-resistant enterococcus isolated from an educational hospital in iran. | 2014 | 25705299 |
| diagnostic and therapeutic utility of transrectal ultrasound in urological office prostatic abscess management: a short report from a single urologic center. | prostatic abscess (pa) is an infrequent condition in the modern antibiotic era. the everyday use of transrectal ultrasound (trus) during diagnostic work-up and the widespread recurrence to prostatic biopsies may lead to an increase of pa diagnosis. in this short report we analyze the patients characteristics and the management of seven recent cases of pa diagnosed in our institution. | 2014 | 25641468 |
| [inferior vena cava thrombosis and endocarditis in a premature patient: case report]. | thrombosis of the inferior vena cava represents a significant percentage of all venous thrombosis that take place during the neonatal period, generally associated with risk factors such as the use of central venous catheter. the incidence of bacterial endocarditis in preterm infants is low. | 2014 | 25697617 |
| antimicrobial activity of root canal irrigants associated with cetrimide against biofilm and planktonic enterococcus faecalis. | to evaluate the antibacterial activity of sodium hypochlorite (naocl) and chlorhexidine (chx) alone or associated with cetrimide (ctr), and qmix against biofilm and planktonic enterococcus faecalis (e. faecalis) [american type culture collection (atcc) 29212]. | 2014 | 25707833 |
| risk factors analysis for occurrence of asymptomatic bacteriuria after endourological procedures. | endourological procedures are performed according to the principles of aseptic techniques, jet still in certain number of patients urinary tract infections may occur. considering the risk of urinary tract infection, there is no unique opinion about the prophylactic use of antibiotics in endourological procedures. | 2014 | 25568546 |
| antibacterial effect of all-in-one self-etch adhesives on enterococcus faecalis. | background and aims. the aim of this study was to evaluate the antibacterial activity of one-step self-etch adhesives on enterococcus faecalis on days 1, 7 and 14 with the use of modified direct contact test. materials and methods. the modified directcontact test was used to evaluate the antibacterial effect of adper easy one, bond force, clearfil s3 bond, futurabond m, g-bond, ibond and optibond all-in-one adhesives on enterococcus faecalis after aging the samples in phosphate-buffered saline f ... | 2014 | 25587384 |
| assessment of photodynamic therapy (pdt) in disinfection of deeper dentinal tubules in a root canal system: an in vitro study. | the success of endodontic treatment therapy depends on how well we eliminate pathogenic microflora from the root canal system as micro organism as the major cause of root canal infection. conventional root canal treatment can fail if microorganisms cannot be removed sufficiently by thorough cleaning, shaping of root canal. newer modalities such as photodynamic therapy are being tried now a days for disinfection of root canals. aim & objectives: the basic aim of this study was assessment of the a ... | 2014 | 25584321 |
| an in vitro evaluation of antimicrobial efficacy of 2% chlorhexidine gel, propolis and calcium hydroxide against enterococcus faecalis in human root dentin. | to evaluate in vitro the antimicrobial efficacy of 2% chlorhexidine gel, propolis and calcium hydroxide against enterococcus faecalis in human root dentin. | 2014 | 25584319 |
| identification and antimicrobial resistance of enterococcus spp. isolated from the river and coastal waters in northern iran. | as fecal streptococci commonly inhabit the intestinal tract of humans and warm blooded animals, and daily detection of all pathogenic bacteria in coastal water is not practical, thus these bacteria are used to detect the fecal contamination of water. the present study examined the presence and the antibiotic resistance patterns of enterococcus spp. isolated from the babolrud river in babol and coastal waters in babolsar. seventy samples of water were collected in various regions of the babolrud ... | 2014 | 25525617 |
| evaluation of photodynamic therapy using a diode laser and different photosensitizers against enterococcus faecalis. | photodynamic therapy (pdt) has been proven to be effective in disinfecting root canals. the aim of this present study was to evaluate the effects of pdt on the viability of enterococcus faecalis using methylene blue (mb) and malachite green (mg) as photosensitizers. solutions containing e. faecalis (atcc 29212) were prepared and harvested by centrifugation to obtain cell suspensions, which were mixed with mb and mg. samples were individually irradiated by the diode laser at a distance of 1mm for ... | 2014 | 25523956 |
| transcriptome analysis of enterococcus faecalis during mammalian infection shows cells undergo adaptation and exist in a stringent response state. | as both a commensal and a major cause of healthcare-associated infections in humans, enterococcus faecalis is a remarkably adaptable organism. we investigated how e. faecalis adapts in a mammalian host as a pathogen by characterizing changes in the transcriptome during infection in a rabbit model of subdermal abscess formation using transcriptional microarrays. the microarray experiments detected 222 and 291 differentially regulated genes in e. faecalis og1rf at two and eight hours after subderm ... | 2014 | 25545155 |
| prevalence of enterococcus faecalis and porphyromonas gingivalis in infected root canals and their susceptibility to endodontic treatment procedures: a molecular study. | because apical periodontitis is recognizably an infectious disease, elimination or reduction of intracanal bacteria is of utmost importance for optimum treatment outcome. | 2014 | 25518530 |
| susceptibility of glycopeptide-resistant enterococci to linezolid, quinupristin/dalfopristin, tigecycline and daptomycin in a tertiary greek hospital. | we investigated the antibiotic susceptibility of glycopeptide-resistant enterococci (gre). seventy consecutive gre were tested. sixty-two isolates were identified as enterococcus faecium (88.6%), and 8 (11.4%) as enterococcus faecalis. all strains were susceptible to linezolid and daptomycin, while 17.1% (12/70) and 11.4% (8/70) were resistant to quinupristin/dalfopristin (qd) and tigecycline, respectively. all e. faecalis isolates were resistant to qd, while 4 of 62 (6.5%) e. faecium isolates w ... | 2014 | 25566405 |
| nanobioarchitectures based on chlorophyll photopigment, artificial lipid bilayers and carbon nanotubes. | in the last decade, building biohybrid materials has gained considerable interest in the field of nanotechnology. this paper describes an original design for bionanoarchitectures with interesting properties and potential bioapplications. multilamellar lipid vesicles (obtained by hydration of a dipalmitoyl phosphatidylcholine thin film) with and without cholesterol were labelled with a natural photopigment (chlorophyll a), which functioned as a sensor to detect modifications in the artificial lip ... | 2014 | 25551059 |
| [clinical efficacy of the antimicrobial drug furamag in nosocomial urinary tract infections]. | to evaluate the efficacy of the combined drug furamag (furasidine potassium and magnesium hydroxycarbonate) in combination with the third-generation cephalosporin cefotaxime versus cephalosporin monotherapy for nosocomial urinary tract infections (nuti). | 2014 | 25509895 |
| [enterococcal infections: from simple to most complex]. | enterococci are microorganisms with a remar- kable ability to adapt to their environment. two species have a significant clinical implication, enterococcus faecalis and enterococcus faecium. the risk factors for colonization and infection must be recognized, including prior treatment with antibiotics such as cephalosporins or quinolones. because of their native resistance to several classes of antibiotics and the increase of acquired resistance to penicillins, the initial empiric treatment of a ... | 2014 | 25507892 |
| disinfection of gutta-percha cones using three reagents and their residual effects. | the purpose of this study was to compare the efficacy of 5.25% sodium hypochlorite, 2% chlorehexidine and mtad in disinfecting gutta-percha cones and to analyze the surface topography of gutta-percha cones after the rapid chemical disinfection procedure. | 2014 | 25506147 |
| enterococcus faecalis from healthy infants modulates inflammation through mapk signaling pathways. | colonizing commensal bacteria after birth are required for the proper development of the gastrointestinal tract. it is believed that bacterial colonization pattern in neonatal gut affects gut barrier function and immune system maturation. studies on the development of faecal microbiota in infants showed that the neonatal gut was first colonized with enterococci followed by other microbiota such as bifidobacterium. other studies showed that babies who developed allergy were less often colonized w ... | 2014 | 24830946 |
| prevalence and detection of mixed-population enterococcal bacteremia. | mixed-population (heterogeneous) enterococcal bacteremia (meb) is rarely reported. based on one occasion in which vitek2 missed a vancomycin-resistant subpopulation isolated from a patient, we developed a simple method to detect this subpopulation and determined meb frequency. the four patients presented here had either enterococcus faecium or enterococcus faecalis bacteremia caused by both vancomycin-resistant enterococci (vre) and vancomycin-susceptible enterococci (vse). no prior common antib ... | 2014 | 24829233 |
| structural and electronic snapshots during the transition from a cu(ii) to cu(i) metal center of a lytic polysaccharide monooxygenase by x-ray photoreduction. | lytic polysaccharide monooxygenases (lpmos) are a recently discovered class of enzymes that employ a copper-mediated, oxidative mechanism to cleave glycosidic bonds. the lpmo catalytic mechanism likely requires that molecular oxygen first binds to cu(i), but the oxidation state in many reported lpmo structures is ambiguous, and the changes in the lpmo active site required to accommodate both oxidation states of copper have not been fully elucidated. here, a diffraction data collection strategy m ... | 2014 | 24828494 |
| iliopsoas abscess in children: report on five patients with a literature review. | we aimed in this study to present the clinical findings in children with iliopsoas abscess (ipa) and to discuss the diagnosis and treatment. the files of five patients, hospitalized between august 2011 and june 2013 and monitored with a diagnosis of ipa, were reviewed retrospectively. demographic characteristics, symptoms and signs, laboratory examinations, and diagnostic and treatment methods of the cases were evaluated. two of the cases were females and three were males, and their ages ranged ... | 2014 | 24827950 |
| isolation and biological activity of compounds from garcinia preussii. | plants of the genus garcinia (clusiaceae) are traditionally used to relieve stomachaches, toothaches, and as a chew stick. | 2014 | 24824323 |
| genome analysis of enterococcus faecalis bacteriophage ime-ef3 harboring a putative metallo-beta-lactamase gene. | lytic enterococcus faecalis bacteriophage ime-ef3 was isolated from hospital sewage, and its genome was sequenced using high-throughput sequencing. genomic analysis and electron microscopy suggested that ime-ef3 was a member of the family siphoviridae. the phage has an isometric head and a long non-contractile tail with a 41 kb linear double-stranded dna genome. the genome encodes 69 putative proteins, with 32 annotated functionally, including proteins related to phage structure, packaging, tran ... | 2014 | 24818695 |
| efficacy of three irrigation agitation techniques on bacterial elimination: a microbiologic and microscopic evaluation. | the aim of this study was to compare in vitro reduction of enterococcus faecalis by using three agitation methods. fifty-five mandibular premolar teeth were used. the root canals were prepared by using protaper instruments to size f4. fresh bacterial suspensions were inoculated into each canal and the teeth were incubated at 37°c under aerobic conditions for 3 weeks. the specimens in negative control group were not contaminated and the teeth in positive control receive no further instrumentation ... | 2014 | 24817336 |
| infliximab therapy increases the frequency of circulating cd16(+) monocytes and modifies macrophage cytokine response to bacterial infection. | crohn's disease (cd) has been correlated with altered macrophage response to microorganisms. considering the efficacy of infliximab treatment on cd remission, we investigated infliximab effects on circulating monocyte subsets and on macrophage cytokine response to bacteria. human peripheral blood monocyte-derived macrophages were obtained from cd patients, treated or not with infliximab. macrophages were infected with escherichia coli, enterococcus faecalis, mycobacterium avium subsp. paratuberc ... | 2014 | 24816497 |
| investigation of mechanism and molecular epidemiology of linezolid-resistant enterococcus faecalis in china. | enterococcus is a major cause of important nosocomial infections. linezolid, the first member of an entirely new class of antibiotics (oxazolidinones), is effective against serious infections caused by enterococcus. however, resistance to linezolid has been discovered throughout the world rapidly. from 2011 to 2013, nine linezolid-resistant e. faecalis isolates were collected and the possible mechanisms of linezolid resistance, including mutations in domain v of 23s rrna genes and in ribosomal p ... | 2014 | 24815727 |
| incidence, risk factors, and outcomes for enterococcus spp. blood stream infections: a population-based study. | enterococci are a clinically significant cause of bloodstream infections (bsi), particularly in the nosocomial setting. the purpose of this study was to characterize the incidence, risk factors for acquisition, microbiological characteristics and mortality of enterococcal bsi within the well-defined population of a large canadian health region. | 2014 | 24813873 |
| role of glycolipids in the pathogenesis of enterococcus faecalis urinary tract infection. | after uropathogenic escherichia coli (upec), enterococcus faecalis is the second most common pathogen causing urinary tract infections. monoglucosyl-diacylglycerol (mglcdag) and diglucosyl-diacylglycerol (dglcdag) are the main glycolipids of the e. faecalis cell membrane. examination of two mutants in genes bgsb and bgsa (both glycosyltransferases) showed that these genes are involved in cell membrane glycolipid biosynthesis, and that their inactivation leads to loss of glycolipids dglcdag (bgsa ... | 2014 | 24806450 |
| activity of daptomycin or linezolid in combination with rifampin or gentamicin against biofilm-forming enterococcus faecalis or e. faecium in an in vitro pharmacodynamic model using simulated endocardial vegetations and an in vivo survival assay using galleria mellonella larvae. | enterococci are the third most frequent cause of infective endocarditis. a high-inoculum stationary-phase in vitro pharmacodynamic model with simulated endocardial vegetations was used to simulate the human pharmacokinetics of daptomycin at 6 or 10 mg/kg of body weight/day or linezolid at 600 mg every 12 h (q12h), alone or in combination with gentamicin at 1.3 mg/kg q12h or rifampin at 300 mg q8h or 900 mg q24h. biofilm-forming, vancomycin-susceptible enterococcus faecalis and vancomycin-resista ... | 2014 | 24867993 |