Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| antigenic and genetic characterization of lipoprotein lppq from mycoplasma mycoides subsp. mycoides sc. | lipoprotein lppq, a predominant 48-kda antigen, and its corresponding gene, lppq, were characterized in mycoplasma mycoides subsp. mycoides sc, the etiological agent of contagious bovine pleuropneumonia. the lppq gene is specific to m. mycoides subsp. mycoides sc and was found in the type strain and in field strains isolated in europe, africa, and australia, as well as in vaccinal strains. lppq is encoded as a precursor with a consensus sequence for prokaryotic signal peptidase ii and a lipid at ... | 2000 | 10882657 |
| influence of hmg-1 and adenovirus oncoprotein e1a on early stages of transcriptional preinitiation complex assembly. | the tata-binding protein (tbp) in the tfiid complex binds specifically to the tata-box to initiate the stepwise assembly of the preinitiation complex (pic) for rna polymerase ii transcription. transcriptional activators and repressors compete with general transcription factors at each step to influence the course of the assembly. to investigate this process, the tbp.tata complex was titrated with hmg-1 and the interaction monitored by electrophoretic mobility shift assays. the titration produced ... | 2000 | 10882737 |
| antiviral activity of shiga toxin 1: suppression of bovine leukemia virus-related spontaneous lymphocyte proliferation. | human infections with shiga toxin (stx)-producing escherichia coli (stec) cause hemorrhagic colitis. the stxs belong to a large family of ribosome-inactivating proteins (rips) that are found in a variety of higher plants and some bacteria. many rips have potent antiviral activity for the plants that synthesize them. stec strains, both virulent and nonvirulent to humans, are frequently isolated from healthy cattle. interestingly, despite intensive investigations, it is not known why cattle carry ... | 2000 | 10899843 |
| rotational mobility and orientational stability of a transport protein in lipid membranes. | a single-cysteine mutant of the lactose transport protein lacs(c320a/w399c) from streptococcus thermophilus was selectively labeled with a nitroxide spin label, and its mobility in lipid membranes was studied as a function of its concentration in the membrane by saturation-transfer electron spin resonance. bovine rhodopsin was also selectively spin-labeled and studied to aid the interpretation of the measurements. observations of spin-labeled proteins in macroscopically aligned bilayers indicate ... | 2000 | 10920009 |
| chalcone and stilbene synthases expressed in eucaryotes exhibit reduced cross-reactivity in vitro. | chalcone synthase (chs) and stilbene synthase (sts) catalyze different cyclization reactions of the common tetraketide to give different products, naringenin chalcone and resveratrol, respectively. we have previously observed in vitro cross-reaction of chs and sts overexpressed in escherichia coli, resveratrol production by chs and chalcone production by sts. when expressed in eucaryotic cells, or in e. coli as thioredoxin-fusion proteins, chs and sts exhibited reduced cross-reaction. sts refold ... | 2000 | 10923839 |
| inhibition of six serine proteinases of the human coagulation system by mutants of bovine pancreatic trypsin inhibitor. | a series of 12 bovine pancreatic trypsin inhibitor variants mutated in the p(4) and p(3) positions of the canonical binding loop containing additional k15r and m52l mutations were used to probe the role of single amino acid substitutions on binding to bovine trypsin and to the following human proteinases involved in blood clotting: plasmin, plasma kallikrein, factors x(a) and xii(a), thrombin, and protein c. the mutants were expressed in escherichia coli as fusion proteins with the le1413 hydrop ... | 2000 | 10930417 |
| p64k meningococcal protein as immunological carrier for weak immunogens. | previously, the p64k meningococcal protein, an antigen of 64 kda expressed in escherichia coli, has been extensively characterized. we have successfully conjugated several synthetic peptides and meningococcal group c polysaccharide to p64k. in three out of four model peptides, the murine humoral immune response against the homologous peptide, evaluated after three doses of conjugate, was higher in the animals immunized with the coupled peptide than in those that received free peptide. the fourth ... | 2000 | 10931377 |
| isolation of shiga toxin-producing escherichia coli including o157:h7 strains from dairy cattle and beef samples marketed in calcutta, india. | 2000 | 10933264 | |
| coupling efficacy and selectivity of the human mu-opioid receptor expressed as receptor-galpha fusion proteins in escherichia coli. | two constructs encoding the human micro-opioid receptor (hmor) fused at its c terminus to either one of two galpha subunits, galpha(o1) (hmor-galpha(o1)) and galpha(i2) (hmor-galpha(i2)), were expressed in escherichia coli at levels suitable for pharmacological studies (0.4-0.5 pmol/mg). receptors fused to galpha(o1) or to galpha(i2) maintained high-affinity binding of the antagonist diprenorphine. affinities of the micro-selective agonists morphine, [d-ala(2),n-me-phe(4),gly(5)-ol]enkephalin (d ... | 2000 | 10936202 |
| enterohaemorrhagic escherichia coli o157:h7 target peyer's patches in humans and cause attaching/effacing lesions in both human and bovine intestine. | enterohaemorrhagic escherichia coli (ehec) constitute a significant risk to human health worldwide, and infections, particularly with serogroup o157:h7, are associated with consumption of a variety of food and water vehicles, particularly food of bovine origin. ehec cause acute gastroenteritis, bloody diarrhoea, and haemorrhagic colitis; up to 10% of cases develop severe complications, including the haemolytic uraemic syndrome, with a 5% case fatality. a virulence characteristic of enteropathoge ... | 2000 | 10940275 |
| overexpression, purification, and characterization of bacillus subtilis n-acetylmuramoyl-l-alanine amidase cwlc. | n-acetylmuramoyl-l-alanine amidase cwlc of bacillus subtilis was overproduced in escherichia coli and purified 21-fold. the amidase hydrolyzed type a cell walls such as b. subtilis. the amidase bound slightly to the microbacterium lacticum cell wall (type b), but did not entirely hydrolyze it. the presence of calcium or magnesium ion increased the resistance of the amidase to heat denaturation. | 2000 | 10945275 |
| a pollen-specific novel calmodulin-binding protein with tetratricopeptide repeats. | calcium is essential for pollen germination and pollen tube growth. a large body of information has established a link between elevation of cytosolic ca(2+) at the pollen tube tip and its growth. since the action of ca(2+) is primarily mediated by ca(2+)-binding proteins such as calmodulin (cam), identification of cam-binding proteins in pollen should provide insights into the mechanisms by which ca(2+) regulates pollen germination and tube growth. in this study, a cam-binding protein from maize ... | 2000 | 10956642 |
| immunological aspects of pregnancy-associated glycoproteins. | the incidence of severe cases of acute e. coli mastitis in dairy cows is highest during early lactation. this phenomenon has been associated with a decreased function and decreased numbers of circulating polymorphonuclear neutrophil leukocytes (pmn). the cause of this impaired function and decreased number is poorly understood. stress, hormonal and metabolic alterations around parturition and the onset of lactation may play a role in this phenomenon. several molecules, such as cortisol and beta- ... | 2000 | 10959437 |
| contribution of dps to acid stress tolerance and oxidative stress tolerance in escherichia coli o157:h7. | an escherichia coli o157:h7 dps::npti mutant (frik 47991) was generated, and its survival was compared to that of the parent in hcl (synthetic gastric fluid, ph 1.8) and hydrogen peroxide (15 mm) challenges. the survival of the mutant in log phase (5-h culture) was significantly impaired (4-log(10)-cfu/ml reduction) compared to that of the parent strain (ca. 1.0-log(10)-cfu/ml reduction) after a standard 3-h acid challenge. early-stationary-phase cells (12-h culture) of the mutant decreased by c ... | 2000 | 10966408 |
| cattle can be a reservoir of sorbitol-fermenting shiga toxin-producing escherichia coli o157:h(-) strains and a source of human diseases. | using the immunomagnetic separation procedure, we isolated sorbitol-fermenting (sf) shiga toxin-producing escherichia coli (stec) o157:h(-) strains from two patients, one with hemolytic-uremic syndrome and the other with diarrhea, and from a dairy cow epidemiologically associated with the patients. the phenotypic and genotypic characteristics of all isolates were identical or closely related. moreover, the bovine isolate showed a clonal relatedness to sf stec o157:h(-) strains isolated from pati ... | 2000 | 10970407 |
| fad insertion is essential for attaining the assembly competence of the dihydrolipoamide dehydrogenase (e3) monomer from escherichia coli. | dihydrolipoamide dehydrogenase (e3) from escherichia coli, an fad-linked homodimer, can be fully reconstituted in vitro following denaturation in 6 m guanidinium chloride. complete restoration of activity occurs within 1-2 h in the presence of fad, dithiothreitol, and bovine serum albumin. in the absence of fad, the dihydrolipoamide dehydrogenase monomer forms a stable folding intermediate, which is incapable of dimerization. this intermediate displays a similar tryptic resistance to the native ... | 2000 | 10970889 |
| shiga-toxin-producing escherichia coli in faeces of healthy dairy cows, sheep and goats: prevalence and virulence properties. | the prevalence of shiga-toxin-producing escherichia coli (stec) in healthy dairy ruminants was investigated between 1996 and 1998 by a multiplex polymerase chain reaction (mpcr) technique. a total of 13 552 e. coli colonies from 726 cows, 28 sheep and 93 goats out of 112 randomly selected dairy farms in hessia, germany were analysed. stec strains were recovered from 131 (18.0%) cows, nine (32.1%) sheep and 70 (75.3%) goats. further characterization of the stec isolates showed that 89 (0.66% of t ... | 2000 | 10972729 |
| frequency of shiga toxin-producing escherichia coli in cattle at a breeding farm and at a slaughterhouse in japan. | to investigate the frequency of shiga toxin-producing escherichia coli (stec) infected calves at a breeding farm and cattle at a slaughterhouse in tohoku area of japan, the polymerase chain reaction (pcr) was used for detection of genes for shiga toxin(s). the fecal samples from a total of 204 calves and 306 cattle were examined. the prevalence rates in calves less than 2 months of age, cattle 2-8 months of age, and adults greater than 1 year of age were 39.4, 78.9, and 40.8%, respectively. dete ... | 2000 | 10973705 |
| evaluation of 1.6% phenol as a premilking and postmilking teat dip in preventing new bovine intramammary infections. | we evaluated the effectiveness of a 1.6% phenol-based teat dip using both a teat skin assay and natural exposure field trial. a teat skin assay was conducted to ascertain the concentration of phenol + phenate to be used in the field study. one percent and 0.5% iodine, and 0.5, 1.1, and 1.6% phenol + phenate were compared using escherichia coli and staphylococcus aureus. logarithmic reductions for s. aureus were 2.2 and 2.8 for 0.5 and 1% iodine, and 1.3, 2.1, and 2.8 for 0.5, 1.1, and 1.6% pheno ... | 2000 | 10984151 |
| plasmid-mediated florfenicol resistance encoded by the flor gene in escherichia coli isolated from cattle. | a florfenicol resistance gene almost identical to flor of salmonella enterica serovar typhimurium dt104 was detected on 110- to 125-kb plasmids in escherichia coli isolates of animal origin. analysis of the flor gene flanking regions of one of the plasmids showed that they were different from those encountered in s. enterica serovar typhimurium dt104. | 2000 | 10991873 |
| g-protein sensitivity of ligand binding to human dopamine d(2) and d(3) receptors expressed in escherichia coli: clues for a constrained d(3) receptor structure. | human dopamine d(2) and d(3) receptors were expressed in chinese hamster ovary (cho) and escherichia coli cells to compare their ligand binding properties in the presence or absence of g-proteins and to analyze their ability to interact with g(i/o)-proteins. binding affinities of agonists (dopamine, 7-oh-dpat, pd128907, lisuride) and antagonists/inverse agonists (haloperidol, risperidone, domperidone, spiperone, raclopride, nemonapride), measured using [(125)i]iodosulpride and [(3)h]7-oh-dpat, w ... | 2000 | 10991990 |
| assessment of the in vitro and in vivo genotoxicity of thalomid (thalidomide). | thalomid is the fda-approved commercial formulation of thalidomide currently used in the us to treat erythema nodosum leprosum, a complication of leprosy. the genotoxicity of thalomid thalidomide was assessed in the ames reverse mutation, as52/xprt mammalian cell forward gene mutation, and mouse bone marrow micronucleus assays. the ames and as52 assays were performed with and without s9. in the ames, salmonella typhimurium strains ta1535, 1537, 98, 100, and 102 and escherichia coli strain wp2 uv ... | 2000 | 10992277 |
| hydrolysis of beta-galactosyl ester linkage by beta-galactosidases. | p-hydroxybenzoyl beta-galactose (phb-gal) was synthesized chemically to examine the hydrolytic activity of beta-galactosyl ester linkage by beta-galactosidases. the enzyme from penicillium multicolor hydrolyzed the substrate as fast as p-nitrophenyl beta-galactoside (pnp-gal), a usual substrate with a beta-galactosidic linkage. the enzymes from escherichia coli and aspergillus oryzae hydrolyzed phb-gal with almost the same rates as pnp-gal. the enzymes from bacillus circulans, saccharomyces frag ... | 2000 | 10993159 |
| short communication: survival of escherichia coli o157:h7 in dairy cattle drinking water. | cattle drinking water from two dairy farms was used in a study to determine the survival characteristics of the bacterial pathogen escherichia coli o157:h7 and wild-type e. coli. the e. coli o157:h7 inoculum consisted of a consortium of isolates obtained from dairy cattle. fresh manure was used as the source for the wild-type e. coli. in the water source from farm 1 the pathogens were present at both 5 and 15 degrees c during the 16-d duration of the study. in the water source from farm 2, the p ... | 2000 | 11003233 |
| comparison between the growth of yersinia enterocolitica, listeria monocytogenes, escherichia coli o157:h7 and salmonella spp. in ground beef packed by three commercially used packaging techniques. | growth of the pathogens yersinia enterocolitica, listeria monocytogenes, escherichia coli o157:h7 and strains of salmonella were compared in ground beef packed in modified atmospheres of 60% co2/40% n2/0.4% co (high co2/low co mixture), 70% o2/30% co2 (high o2 mixture) and in chub packs (stuffed in plastic casings). the ground beef was inoculated with rifampicin-resistant or nalidixic acid/streptomycin-resistant strains of the pathogens (final concentration 10(2) - 10(3) bacteria/g) and stored a ... | 2000 | 11020041 |
| the isolation of enterohaemorrhagic escherichia coli o111:h- from australian cattle. | 2000 | 11022293 | |
| interactions of the nadp(h)-binding domain iii of proton-translocating transhydrogenase from escherichia coli with nadp(h) and the nad(h)-binding domain i studied by nmr and site-directed mutagenesis. | using the purified nadp(h)-binding domain of proton-translocating escherichia coli transhydrogenase (eciii) overexpressed in (15)n- and (2)h-labeled medium, together with the purified nad(h)-binding domain from e. coli (eci), the interface between eciii and eci, the nadp(h)-binding site and the influence on the interface by nad(p)(h) was investigated in solution by nmr chemical shift mapping. mapping of the nadp(h)-binding site showed that the nadp(h) substrate is bound to eciii in an extended c ... | 2000 | 11027139 |
| one-step purification of the recombinant catalytic subunit of pyruvate dehydrogenase phosphatase. | a facile one-step affinity chromatographic purification of the recombinant catalytic subunit (pdpc) of bovine pyruvate dehydrogenase phosphatase (pdp) to near homogeneity is described. pdpc binds in the presence of ca(2+) to the inner lipoyl domain (l2) of the dihydrolipoamide acetyltransferase component (e2) of the mammalian pyruvate dehydrogenase complex. the affinity column consists of a glutathione s-transferase (gst)-l2 fusion protein bound to glutathione-sepharose 4b beads. an extract of t ... | 2000 | 11035961 |
| characterization of shiga toxin-producing escherichia coli strains isolated from calves in poland. | fecal samples from 67 3-5-months-old calves with diarrhea were screened for the presence of shiga toxin-producing escherichia coli (stec). several accessory virulence factors genes were also tested. among 192 e. coli isolates tested, 15 (7.6%) were found to harbour the shiga toxin 1 or 2 (stx1 or stx2) genes. the stx2-carrying samples were further subtyped by pcr for the stx2c, stx2d, and stx2e toxin variants. it was shown that stx2-positive bacteria mainly possessed the stx2c shiga toxin type g ... | 2000 | 11038128 |
| an optimized recipe for cloning of the polymerase chain reaction-amplified dna inserts into plasmid vectors. | this study compares a number of parameters that are important in the ligation of the polymerase chain reaction-amplified dna inserts into plasmid vectors and their efficient transformation to bacterial cells. the parameters covered were: t4 polynucleotide kinase treatment followed by either the large fragment of e. coli dna polymerase or t4 dna polymerase reactions, the amount of t4 dna ligase, temperature and duration of ligation, molar ratio of insert to vector as well as the total dna concent ... | 2000 | 11310983 |
| phenylarsine oxide inhibits nitric oxide synthase in pulmonary artery endothelial cells. | the role of protein tyrosine phosphorylation during regulation of no synthase (enos) activity in endothelial cells is poorly understood. studies to define this role have used inhibitors of tyrosine kinase or tyrosine phosphatase (tp). phenylarsine oxide (pao), an inhibitor of tp, has been reported to bind thiol groups, and recent work from our laboratory demonstrates that enos activity depends on thiol groups at its catalytic site. therefore, we hypothesized that pao may have a direct effect on ... | 2000 | 11281283 |
| biological activities of bovine glycomacropeptide. | biological activity of bovine kappa-caseino glycomacropeptide (gmp) has received much attention in recent years. research has focused on the ability of gmp to bind cholera and escherichia coli enterotoxins, inhibit bacterial and viral adhesion, suppress gastric secretions, promote bifidobacterial growth and modulate immune system responses. of these, protection against toxins, bacteria, and viruses and modulation of the immune system are the most promising applications. | 2000 | 11242445 |
| survival of salmonella typhimurium and escherichia coli o157:h7 in poultry manure and manure slurry at sublethal temperatures. | exponential inactivation was observed for salmonella typhimurium and escherichia coli o157:h7 in poultry manure with decimal reduction times ranging from half a day at 37 c to 1-2 wk at 4 c. there was no material difference in inactivation rates between s. typhimurium and e. coli o157:h7. inactivation was slower in slurries made by mixing two parts of water with one part of manure; decimal reduction times (time required for 90% destruction) ranged from 1-2 days at 37 c to 6-22 wk at 4 c. escheri ... | 2000 | 11195639 |
| [action of natural gamma-interferons on functional activity of phagocytes and antibody synthesis after vaccination]. | natural swine and cattle gamma-ifns were prepared for trials. one dose of gamma-suiferon contained 1000 iu, that of gamma-boviferon--2000 iu. three series of researches were carried out to estimate the in vitro and in vivo absorbing activity of phagocytes (monocytes and neutrophiles), their bactericidal ability (on new born pigs and calves, 2 months old animals, sows and cows with calf) and antibodygenesis after immunization of animals by colibacteriosis vaccine. it has been shown in trials that ... | 2000 | 11247346 |
| [detection of escherichia coli strains harboring pathogenicity island of yersinia enterolitica in diarrheal patients and animals in china]. | to investigate the prevalence of escherichia coil strains harboring high pathogenicity island of yersinia enterocolitica in diarrhea patients, animals, food samples and resulted clinical symptoms. | 2000 | 11860774 |
| microbiological contamination of raw beef trimmings and ground beef. | to survey the microbiological quality of beef trimmings and final-ground beef, samples were collected from eight commercial grinding facilities, including trimmings from fed-cattle, culled-beef cows, culled-dairy cows, imported-beef trimmings and finished-ground products. trim samples (core and purge) and ground product samples (n=586) were evaluated for aerobic plate (apc), total coliform (tcc), escherichia coli (ecc) and staphylococcus aureus counts and the presence of salmonella spp. and list ... | 2000 | 22061902 |
| rapid and sensitive detection of escherichia coli o157:h7 in bovine faeces by a multiplex pcr. | cattle are considered the major reservoir for escherichia coli o157:h7, one of the newly emerged foodborne human pathogens of animal origin and a leading cause of haemorrhagic colitis in humans. a sensitive test that can accurately and rapidly detect the organism in the food animal production environment is critically needed to monitor the emergence, transmission, and colonization of this pathogen in the animal reservoir. in this study, a novel multiplex polymerase chain reaction (pcr) assay was ... | 1999 | 10664910 |
| purification and characterization of chitin-binding proteins from the hemolymph of sweet potato hornworm, agrius convolvuli. | three chitin-binding proteins (cbps: cbp9, cbp15, cbp66) were identified from the larval hemolymph of sweet potato hornworm, agrius convolvuli. two (cbp9 and cbp15) of them have been isolated and purified by gel filtration (superdex hr 75), cation-exchange chromatography (mono s), and reverse-phase chromatography (murpc pc 2.1/3). in experiments to detect cbps in hemolymph, we examined whether ionic strength and existence of bovine serum albumin in the incubation solution influenced binding affi ... | 1999 | 10665375 |
| the effects of the surface charge and hydrophobicity of escherichia coli on its adhesion to beef muscle. | the surface characteristics of escherichia coli strains were studied to evaluate the effect upon bacterial adhesion to beef muscle. the influence of suspension conditions upon the surface charge of a pathogenic strain, e. coli o157:h7 (ec01), and a saprophytic laboratory strain, e. coli jm109 (ec22) were investigated and compared. the cellular surface charge of most e. coli o157:h7 strains were much less affected by changes in the ph, ionic strength or concentration of surfactants in the suspend ... | 1999 | 10634709 |
| phosphoserine aminotransferase, the second step-catalyzing enzyme for serine biosynthesis. | as a step toward analyzing the serine biosynthetic pathway in mammals, we have studied the properties of phosphoserine aminotransferase, the second step-catalyzing enzyme. the k(m) values for 3-phosphohydroxypyruvate and l-phosphoserine are 5 and 35 microm, respectively, and those for glutamate and alpha-ketoglutarate are 1.2 and 0.8 mm, respectively. the product inhibition studies strengthened the support for a ping-pong mechanism and allowed evaluation of ki values for the four substrates. the ... | 1999 | 10637769 |
| vaccines and diagnostic methods for bovine mastitis: fact and fiction. | a number of problems are uniquely associated with vaccination of dairy cows for mastitis. one of these is that the number of mastitis pathogens is numerous and heterogeneous. vaccine efforts have concentrated mainly on the major mastitis pathogens. while at least one s. aureus bacterin has been commercially available for a number of years, no large-scale, independent field trials have been published in refereed journals which support the efficacy of this vaccine. experimental vaccines for s. aur ... | 1999 | 9890021 |
| transcriptional inhibition of the operon for the spermidine uptake system by the substrate-binding protein potd. | inhibition of spermidine uptake in escherichia coli, which occurs in the presence of accumulated polyamines, has been studied using the spermidine uptake operon consisting of the pota, -b, -c, and -d genes. transcription of the potabcd operon was inhibited by potd, a spermidine-binding protein usually found in the periplasm, and the inhibitory effect of potd was increased by spermidine. transcription was not affected by bovine serum albumin, pota, or potf, suggesting that the effects of potd are ... | 1999 | 9890949 |
| roles of pilc and pile proteins in pilus-mediated adherence of neisseria gonorrhoeae and neisseria meningitidis to human erythrocytes and endothelial and epithelial cells. | unlike other type 4 pili, the neisserial pili consist of at least two distinct proteins, the highly variable major subunit pile forming the pilus fiber and the tip-associated adhesin pilc. pilc protein purified either from gonococci or from escherichia coli interacted with different human epithelial cell lines, primary epithelial and endothelial cells. the binding of pilc protein efficiently prevented the attachment of piliated neisseria gonorrhoeae and neisseria meningitidis to these cell types ... | 1999 | 9916098 |
| role of carboxyl-terminal charges on s-modulin membrane affinity and inhibition of rhodopsin phosphorylation. | s-modulin shows a higher affinity for urea-stripped frog rod outer segment membranes than s26 (a cone homologue of s-modulin). nacl at a concentration of several hundred millimolar reduced the membrane affinity of s-modulin to the s26 level. chimeric s-modulin and s26 whose respective 23 and 29 amino acids at the carboxyl terminus were swapped showed membrane affinites similar to those of s26 and s-modulin, respectively. the membrane affinity of an s-modulin mutant lacking c-terminal positive ch ... | 1999 | 9930992 |
| cryptosporidium and concurrent infections with other major enterophatogens in 1 to 30-day-old diarrheic dairy calves in central spain. | faeces samples from 218, 1 to 30-day-old, diarrheic dairy calves in 65 dairy herds were screened for the presence of cryptosporidium and concurrent infections with rotavirus, coronavirus, f5 escherichia coli and salmonella spp. calves were grouped according to their age as follows: 1-7, 8-14, 15-21 and 22-30 days. cryptosporidium infection was detected in 43.8%, 71.9%, 63.2% and 6.9% of the calves in the respective age groups. significant differences in the detection rate of cryptosporidium were ... | 1999 | 9950342 |
| pregnancy-associated glycoprotein and decreased polymorphonuclear leukocyte function in early post-partum dairy cows. | phagocytosis and oxidative burst activity of polymorphonuclear neutrophil leukocytes (pmn) isolated from blood and pregnancy-associated glycoprotein (bpag) concentrations in plasma were evaluated in two longitudinal studies in dairy cows from 3 weeks before until 5 weeks after calving, carried out in the united states and in europe. ingestion of staphylococcus aureus by blood pmn increased during the first week after calving and normalised 3 weeks post-partum. phagocytosis of escherichia coli di ... | 1999 | 9950353 |
| recurrent clinical mastitis caused by escherichia coli in dairy cows. | in this study, the occurrence of persistent intramammary infections caused by escherichia coli with recurrent episodes of clinical mastitis caused by e. coli are described for a cohort of 300 dutch dairy herds. calculations on the recurrent episodes were based on data collected by dairy farmers. the genotype of the e. coli strains was determined by means of a polymerase chain reaction using enterobacterial repetitive intergenic consensus (eric) primers, resulting in a dna fingerprint. quarters i ... | 1999 | 10022009 |
| influence of oxygen availability on physiology, verocytotoxin expression and adherence of escherichia coli o157. | a strain of escherichia coli serotype o157 was grown in steady state chemostat culture under aerobic, oxygen-limited and anaerobic conditions. the growth and metabolic efficiency of oxygen-limited and anaerobic cultures was impaired, with biomass yield and the molar growth yield for glucose, yglucose, reduced markedly in comparison with aerobic cultures. steady state cells were typically short rods 2-3 microns long, and were encapsulated by a layer of extracellular material. the majority of cell ... | 1999 | 10030015 |
| escherichia coli o157:h7 in retail ground beef in seattle: results of a one-year prospective study. | escherichia coli o157:h7 was sought systematically in 1,400 samples of retail ground beef in seattle in a 1-year prospective study. sorbitol-nonfermenting, lactose-fermenting, indole-positive colonies isolated after enrichment culture were probed for the presence of shiga toxin genes. totals of 67,040 sorbitol-nonfermenting and 66,705 sorbitol-fermenting colonies were characterized, but e. coli o157:h7 was not identified. the sensitivity of this technique was usually sufficient to detect e. coli ... | 1999 | 10030631 |
| cytochrome p450c17-expressing escherichia coli as a first-step screening system for 17alpha-hydroxylase-c17,20-lyase inhibitors. | we have designed and synthesized a number of cytochrome p450 17alpha-hydroxylase-c17,20-lyase (p450c17) inhibitors with the aim of inhibiting androgen synthesis. to select the most potent inhibitors, we initially used human testicular microsomes, which have a high level of expression of this enzyme. however, due to lack of availability of human tissue and variability among the samples, we utilized recombinant human enzyme expressed in escherichia coli. we designed a simple and economical protoco ... | 1999 | 10036137 |
| assessment of the adequacy of cleaning of equipment used for breaking beef carcasses. | enumeration of bacteria on product entering or leaving a beef carcass breaking process showed that the meat was being contaminated with escherichia coli during the process. the equipment used in the process appeared to be well cleaned, and few bacteria were recovered from meat-contacting surfaces of cleaned equipment. however, careful inspection of the cleaned equipment revealed obscured locations in the equipment which harboured detritus that carried large numbers of aerobic bacteria including ... | 1999 | 10050680 |
| collaborative evaluation of detection methods for escherichia coli o157:h7 from radish sprouts and ground beef. | for the evaluation of plating and immunological methods applicable to the detection of escherichia coli o157:h7 from ground beef and radish sprouts, a collaborative study was conducted. it focused on a comparison of the efficiency of the plating and immunological methods using various plating agars and immuno-kits in combination with enrichment in modified e. coli broth supplemented with novobiocin (mec + n), and using immunomagnetic separation. the plating media tested were sorbitol macconkey a ... | 1999 | 10050682 |
| characterization of rpos alleles in escherichia coli o157:h7 and in other e. coli serotypes. | the rpos nucleotide and predicted amino acid sequences from three escherichia coli o157:h7 isolates were compared with those from three other e. coli isolates, including the likely o157:h7 progenitor, e. coli o55:h7. these clinical and environmental isolates all had identical sigma s amino acid sequences, while laboratory strains k12 and dh1 had three and one amino acid alterations, respectively, in comparison with the majority sequence. to extend the analysis of sigma s sequence conservation to ... | 1999 | 10063629 |
| detection of escherichia coli o157:h7 from musca domestica (diptera: muscidae) at a cattle farm in japan. | enterohemorrhagic escherichia coli (ehec) o157:h7 was isolated for the first time from musca domestica l. a total of 310 fly samples was collected from 4 different farms in obihiro-city, hokkaido, in the summer and autumn of 1997;5 samples carried e. coli serotype o157:h7. using elisa and vero cell cytotoxicity assay, 3 isolates from 1 cattle farm produced both active shiga-toxin type 1 (stx1) and 2 (stx2). these isolates also carried hemolysin and eaea genes and harbored the 90-kb virulence pla ... | 1999 | 10071501 |
| modulation of the remote heme site geometry of recombinant mouse neuronal nitric-oxide synthase by the n-terminal hook region. | the role of two essential residues at the n-terminal hook region of neuronal nitric-oxide synthase (nnos) in nitric-oxide synthase activity was investigated. full-length mouse nnos proteins containing single-point mutations of thr-315 and asp-314 to alanine were produced in the escherichia coli and baculovirus-insect cell expression systems. the molecular properties of the mutant proteins were analyzed in detail by biochemical, optical, and electron paramagnetic resonance spectroscopic technique ... | 1999 | 10075660 |
| pattern of diarrhoea in newborn beef calves in south-west france. | a prospective study was carried out on 94 randomly selected beef herds in the midi-pyrénées region in france. the objective was to describe diarrhoea and mortality in beef calves from birth to 30 days of age. calves (3,080) were followed from december 1995 to april 1996, and a total of 700 visits allowed records of herd management practices, individual data and environmental conditions to be collected. the incidence rate for diarrhoea during the neonatal period was 14.6%, and varied markedly bet ... | 1999 | 10081113 |
| expression of bovine cytokines in escherichia coli. | glutathione s-transferase fusion proteins of bovine interleukin-2 (il-2), il-4, il-6 and interferon-gamma (ifn-gamma) were expressed in escherichia coli. complementary dna (cdna) for open reading frame of each cytokine without signal peptide encoding region was amplified by reverse transcriptional polymerase chain reaction method and was subcloned into pgex-5x-1. in result, il-6 and ifn-gamma fusion proteins in bacteria were soluble, but il-2 and il-4 fusion proteins were insoluble. the insolubl ... | 1999 | 10081758 |
| lipopolysaccharide enhances the production of vascular endothelial growth factor by human pulp cells in culture. | we investigated whether vascular endothelial growth factor (vegf) production by human pulp cells (hpc) is regulated by lipopolysaccharide (lps) in relation to the pathogenesis of pulpitis. although hpc incubated with medium alone only marginally expressed vegf mrna and produced a low level of vegf as detected by enzyme-linked immunosorbent assay, the vegf mrna expression and vegf production were markedly enhanced upon stimulation with lps from escherichia coli. prevotella intermedia lps, phorbol ... | 1999 | 10084996 |
| effects of ph and acid resistance on the radiation resistance of enterohemorrhagic escherichia coli. | the effects of ph and the induction of ph-dependent stationary-phase acid resistance on the radiation resistance of escherichia coli were determined for seven enterohemorrhagic strains and one nonenterohemorrhagic strain. the isolates were grown in acidogenic or nonacidogenic media to ph levels of approximately 4.7 and 7.2, respectively. the cells were then transferred to brain heart infusion (bhi) broth adjusted to ph 4.0, 4.5, 5.0, and 5.5 (with hcl) that was preequilibrated to 2 degrees c, an ... | 1999 | 10090239 |
| a recombinant bovine gallbladder mucin polypeptide binds biliary lipids and accelerates cholesterol crystal appearance time. | mucin has a central role in the pathogenesis of cholesterol gallstones, in part because of its ability to bind biliary lipids and accelerate cholesterol crystal appearance time. previous studies have localized these properties to nonglycosylated mucin domains, and we have recently shown that these domains contain a series of 127-amino acid, cysteine-rich repeats. the aim of this study was to express a recombinant mucin polypeptide containing these repeats and investigate its lipid-binding and pr ... | 1999 | 10092316 |
| characterization of the cardiac holotroponin complex reconstituted from native cardiac troponin t and recombinant i and c. | cardiac troponin i (ctni), the inhibitory subunit of cardiac troponin (ctn), is phosphorylated by the camp-dependent protein kinase a at two adjacently located serine residues within the heart-specific n-terminal elongation. four different phosphorylation states can be formed. to investigate each monophosphorylated form ctni mutants, in which each of the two serine residues is replaced by an alanine, were generated. these mutants, as well as the wild-type cardiac troponin i (ctni-wt) have been e ... | 1999 | 10103031 |
| real-time monitoring of escherichia coli o157:h7 adherence to beef carcass surface tissues with a bioluminescent reporter. | a method for studying bacteria that are attached to carcass surfaces would eliminate the need for exogenous sampling and would facilitate understanding the interaction of potential human food-borne pathogens with food animal tissue surfaces. we describe such a method in which we used a bioluminescent reporter strain of escherichia coli o157:h7 that was constructed by transformation with plasmid pcgls1, an expression vector that contains a complete bacterial luciferase (lux) operon. beef carcass ... | 1999 | 10103275 |
| catalase hpii from escherichia coli exhibits enhanced resistance to denaturation. | catalase hpii from escherichia coli is a homotetramer of 753 residue subunits. the multimer displays a number of unusual structural features, including interwoven subunits and a covalent bond between tyr415 and his392, that would contribute to its rigidity and stability. as the temperature of a solution of hpii in 50 mm potassium phosphate buffer (ph 7) is raised from 50 to 92 degrees c, the enzyme begins to lose activity at 78 degrees c and 50% inactivation has occurred at 83 degrees c. the ina ... | 1999 | 10194300 |
| a mutation in the heterotrimeric stimulatory guanine nucleotide binding protein alpha-subunit with impaired receptor-mediated activation because of elevated gtpase activity. | it has been reported that substitution of arg258, a residue within the gtpase domain of the heterotrimeric guanine nucleotide binding protein (g protein) alpha-subunit (alphas), to alanine (alphas-r258a) results in decreased activation by receptor or aluminum fluoride (alf4-) and increased basal gdp release. arg258 interacts with gln170 in the helical domain, and, presumably, loss of this interaction between the gtpase and helical domain leads to more rapid gdp release, resulting in decreased ac ... | 1999 | 10200251 |
| chloroplast nadh dehydrogenase from pisum sativum: characterization of its activity and cloning of ndhk gene. | the pea chloroplast ndhk gene coding for a component of a nadh-plastoquinone oxidoreductase has been cloned and sequenced. this gene codes for a polypeptide of 227 amino acids and a predicted molecular mass of 25,495 da which belongs to the family of the 20 kda psst subunit of the bovine mitochondrial complex i. a fragment of this gene has been overexpressed in escherichia coli, and antibodies against the expressed polypeptide recognize a protein of the predicted molecular mass from pea thylakoi ... | 1999 | 10202810 |
| detection of multidrug-resistant salmonella enterica serotype typhimurium dt104 based on a gene which confers cross-resistance to florfenicol and chloramphenicol. | salmonella enterica serotype typhimurium (s. typhimurium) dt104 (dt104) first emerged as a major pathogen in europe and is characterized by its pentadrug-resistant pattern. it has also been associated with outbreaks in the united states. the organism typically carries resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, and tetracycline. the mechanism of chloramphenicol resistance in dt104 was determined by producing antibiotic-resistant escherichia coli host strain clones from ... | 1999 | 10203484 |
| effect of antibiotic induced bacterial clearance in the udder on l-selectin shedding of blood neutrophils in cows with escherichia coli mastitis. | bacterial clearance, l-selectin adhesion receptor expression and neutrophil recruitment during experimentally induced escherichia coli mastitis were investigated. acute mastitis was induced by injection of 10(4) cfu e. coli into both left quarters of 12 clinically healthy lactating cows, 2-6 weeks after parturition. half the cows were treated intravenously 10 h after infection, and subcutaneously 30 h after infection with enrofloxacin. in both groups, bacterial count, milk serum albumin, milk so ... | 1999 | 10206204 |
| antibacterial activity of gentamicin and ciprofloxacin against gram-negative bacteria: interactions with pig and calf sera. | the antibacterial activity of pig and calf serum and its ability to interact with gentamicin and ciprofloxacin were studied in vitro using escherichia coli k-12, proteus rettgeri (sanelli) and klebsiella pneumoniae atcc 10031. the antimicrobial activity of the above drugs, alone or in combination with serum, was investigated by the checkerboard method and expressed as the minimal inhibitory concentration (microg ml-1). pig serum (25%) with gentamicin had a synergistic antibacterial effect agains ... | 1999 | 10208763 |
| unusual sites of arginine methylation in poly(a)-binding protein ii and in vitro methylation by protein arginine methyltransferases prmt1 and prmt3. | arginine methylation is a post-translational modification found mostly in rna-binding proteins. poly(a)-binding protein ii from calf thymus was shown by mass spectrometry and sequencing to contain ng, ng-dimethylarginine at 13 positions in its amino acid sequence. two additional arginine residues were partially methylated. almost all of the modified residues were found in arg-xaa-arg clusters in the c terminus of the protein. these motifs are distinct from arg-gly-gly motifs that have been previ ... | 1999 | 10224081 |
| molecular cloning of a novel human collectin from liver (cl-l1). | collectins are a c-lectin family with collagen-like sequences and carbohydrate recognition domains. these proteins can bind to carbohydrate antigens of microorganisms and inhibit their infection by direct neutralization and agglutination, the activation of complement through the lectin pathway, and opsonization by collectin receptors. here we report the cloning of a cdna encoding human collectin from liver (cl-l1 (collectin liver 1)) that has typical collectin structural characteristics, consist ... | 1999 | 10224141 |
| shiga toxin 1 from escherichia coli blocks activation and proliferation of bovine lymphocyte subpopulations in vitro. | shiga toxin-producing escherichia coli (stec) is widespread in the cattle population, but the clinical significance of shiga toxins (stx's) for the bovine species remains obscure. since stx's exert immunomodulating effects in other species, we examined the effect of purified stx1 on a bovine b lymphoma cell line (bl-3) and peripheral blood mononuclear cells (pbmc) isolated from adult bovine blood by viability assays and flow cytometry analysis. stx1 markedly induced apoptosis in stimulated bl-3 ... | 1999 | 10225876 |
| interactions of the papovavirus dna replication initiator proteins, bovine papillomavirus type 1 e1 and simian virus 40 large t antigen, with human replication protein a. | papovaviruses utilize predominantly cellular dna replication proteins to replicate their own viral genomes. to appropriate the cellular dna replication machinery, simian virus 40 (sv40) large t antigen (tag) binds to three different cellular replication proteins, the dna polymerase alpha-primase complex, the replication protein a (rpa) complex, and topoisomerase i. the functionally similar papillomavirus e1 protein has also been shown to bind to the dna polymerase alpha-primase complex. enzyme-l ... | 1999 | 10233951 |
| patterning cells and their environments using multiple laminar fluid flows in capillary networks. | this paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. we demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. this method offers ... | 1999 | 10318920 |
| evaluation of the role of endotoxin and cortisol on modulation of cd18 adhesion receptors in cows with mastitis caused by escherichia coli. | to determine the effect of mastitis caused by escherichia coli on expression of cd18 cell surface receptors and to evaluate the involvement and regulation of receptors by lipopolysaccharide (lps) and cortisol. | 1999 | 10328420 |
| expression of recombinant bovine l-selectin in escherichia coli and insect cells. | bovine l-selectin was expressed in bacteria using pgex vector and in insect cells infected with recombinant baculovirus in order to obtain recombinant protein for preparation of specific antiserum and its functional studies. in bacterial expression, l-selectin fusion protein with glutathione s-transferase was detected in the insoluble fraction with the expected molecular weight of 60 kda by sds-page and reacted with anti-bovine cd62l monoclonal antibody in immunoblot analysis. in insect cells in ... | 1999 | 10331197 |
| activation of toxin adp-ribosyltransferases by eukaryotic adp-ribosylation factors. | adp-ribosylation factors (arfs) are members of a multigene family of 20-kda guanine nucleotide-binding proteins that are regulatory components in several pathways of intracellular vesicular trafficking. the relatively small (approximately 180-amino acids) arf proteins interact with a variety of molecules (in addition to gtp/gdp, of course). cholera toxin was the first to be recognized, hence the name. later it was shown that arf also activates phospholipase d. different parts of the molecule are ... | 1999 | 10331652 |
| recombinant chicken ifn-gamma expressed in escherichia coli: analysis of c-terminal truncation and effect on biologic activity. | interferon-gamma (ifn-gamma) possesses potent immunostimulatory properties, and it has recently been shown to have potential therapeutic properties. recombinant protein technology is frequently used for commercial production of therapeutics, such as ifn. biologically active recombinant chicken ifn-gamma (rchifn-gamma) constructs bearing an n-terminal poly-his tag were expressed in escherichia coli. preparations of rchifn-gamma contained varying ratios of a full-length and a truncated protein spe ... | 1999 | 10334390 |
| genomic dna restriction site heterogeneity in bovine pasteurella multocida serogroup a isolates detected with an rrna probe. | a total of 81 pasteurella multocida isolates from healthy and diseased dairy and beef cattle originating from various geographical locations was examined by rrna gene restriction site polymorphism analysis (ribotyping), restriction endonuclease analysis (rea), sds-page analysis of whole-cell (wcp) and outer-membrane (omp) proteins, and capsule and somatic serotyping. bacterial strains were isolated from nose, lung and in one case testicle, of holstein and cross-bred beef cattle. the isolates rep ... | 1999 | 10334595 |
| new general approach for determining the solution structure of a ligand bound weakly to a receptor: structure of a fibrinogen aalpha-like peptide bound to thrombin (s195a) obtained using noe distance constraints and an ecepp/3 flexible docking program. | a new approach incorporating flexible docking simulations and nmr data is presented for calculating the bound conformation of a ligand that interacts weakly with an enzyme. this approach consists of sampling directly the conformation of a flexible ligand inside a receptor active site containing surrounding flexible loops. to make this sampling efficient, a ligand-growing procedure has been adopted. optimization of the ecepp/3-plus-noe constraint function is carried out by using a collective vari ... | 1999 | 10336381 |
| [detection of pas gene in shiga toxin producing escherichia coli (stec)]. | results of detection of the pas gene in 10 shigatoxin-producing escherichia coli strains (stec) isolated from foods (raw milk, certified milk, and beef) and in 18 enterohaemorrhagic escherichia coli strains (ehec) isolated from stool samples of patients suffering from hus, diarrhea or from carriers without symptoms are given. all isolates showed the eae gene coding for intimin. we could make sure by using pcr that all isolates showed the pas gene, too. this gene is a factor mainly responsible fo ... | 1999 | 10337054 |
| the use of non-immune plasma powder in the prophylaxis of neonatal escherichia coli diarrhoea in calves. | the protective use of plasma powder from cattle and swine against experimentally induced neonatal e. coli diarrhoea in colostrum-deprived calves was examined. diarrhoea was induced with a strain expressing f5+ fimbriae and a strain expressing f17+ fimbriae. in all groups supplemented with bovine plasma powder, diarrhoea and fever were less severe than in the control groups. for the groups infected with the f5+ e. coli strain, a reduction in excretion of the challenge strain by 2-4 orders of magn ... | 1999 | 10337234 |
| growth of starved escherichia coli o157 cells in selective and non-selective media. | escherichia coli o157 strains starved in sterile deionized water (sdw) and filter-sterilized natural river water (srw) were investigated with specific reference to their culturability in selective and non-selective media. growth of the strains starved in both sdw and srw were markedly suppressed with time in selective liquid media such as modified trypticase soy broth supplemented with novobiocin (mtsb+n) and modified e. coli broth supplemented with novobiocin (mec+n). this suppression was more ... | 1999 | 10338190 |
| incidence of salmonella on beef carcasses relating to the u.s. meat and poultry inspection regulations. | this article is part of a major study designed to collect baseline contamination data by sampling beef carcasses in seven slaughtering plants (four steer-heifer and three cow-bull plants) during both a dry season (november to january) and a wet season (may to june). samples (n = 30) were excised from each of three carcass anatomical sites (brisket, flank, and rump) at each of three points in the slaughtering chain (pre-evisceration, following final carcass washing, after 24-h carcass chilling). ... | 1999 | 10340666 |
| infection of gnotobiotic calves with escherichia coli o157:h7 strain a84. | six colostrum-deprived, hysterotomy-derived calves were maintained under sterile conditions and fed a milk replacer diet. at five days of age, five of the calves were dosed orally with 10(9) cfu of escherichia coli o157:h7 strain a84. they were killed after, one, two, six, 12 and 24 days, and samples were taken for bacteriological and pathological examination. the sixth uninfected control calf was killed at seven days of age and matched samples were taken for pathological comparison. the animals ... | 1999 | 10358875 |
| identification of the rna-binding, dimerization, and eif4gi-binding domains of rotavirus nonstructural protein nsp3. | the rotavirus nonstructural protein nsp3 is a sequence-specific rna binding protein that binds the nonpolyadenylated 3' end of the rotavirus mrnas. nsp3 also interacts with the translation initiation factor eif4gi and competes with the poly(a) binding protein. deletion mutations and point mutations of nsp3 from group a rotavirus (nsp3a), expressed in escherichia coli, indicate that the rna binding domain lies between amino acids 4 and 149. similar results were obtained with nsp3 from group c rot ... | 1999 | 10364288 |
| afa and f17 adhesins produced by pathogenic escherichia coli strains in domestic animals. | afa and f17 are afimbrial and fimbrial adhesins, respectively, produced by pathogenic escherichia coli strains in domestic animals. f17-related fimbriae are mainly detected on bovine and ovine e. coli associated with diarrhoea or septicaemia. the f17-g adhesin subunits recognize n-acetyl-d-glucosamine (glcnac) receptors present on bovine intestinal cells. some f17 subtypes also bind to glcnac receptors present on human uroepithelial and intestinal caco-2 cells or to the laminin contained in the ... | 1999 | 10367361 |
| [udder health on dairy farms. 1. results of a longitudinal study on 300 dutch farms]. | udder health was studied in 300 dairy herds grouped in three categories according to the bulk milk somatic cell count. in all herds, lactating cows were housed in a free-stall barn during the winter. all herds participated in a three or four-weekly milk recording system, had annual production quota between 300,000 and 900,000 kg, and were stocked with cows of the holstein-friesian or dutch friesian breeds. the incidence of clinical mastitis was not different among herds with a low (< or = 150,00 ... | 1999 | 10372420 |
| role of lactosyl glycan sequences in inhibiting enteropathogenic escherichia coli attachment. | previously, we found that asialo-lactosamine sequences served as receptors for enteropathogenic escherichia coli (epec) binding to chinese hamster ovary (cho) cells. in the present report, we have extended these earlier results by examining the ability of lactosamine- or fucosylated lactosamine-bovine serum albumin (bsa) glycoconjugates to inhibit epec, strain e2348/69, binding to hep-2 cells. we found that, consistent with our previous findings with cho cells, n-acetyllactosamine-bsa was the mo ... | 1999 | 10377105 |
| assessment of the microbiological conditions of tails, tongues, and head meats at two beef-packing plants. | newly skinned tails of beef carcasses at two packing plants were similarly contaminated with total aerobes and with coliforms that were largely escherichia coli at log mean numbers about 3.5/cm2 and 4.5/100 cm2, respectively. the log mean numbers of aerobes and coliforms on the skinned tails after washing at plant a were, respectively, 1 and 2 log units less than the numbers on the newly skinned tails. at plant b, the log mean numbers of aerobes on skinned and on washed tails were similar while ... | 1999 | 10382660 |
| [prevalence and serotypes of verocytotoxin-producing escherichia coli (vtec) isolates from dairy cattle]. | to clarify the source of infection and route of transmission of verocytotoxin-producing escherichia coli (vtec) in humans, we collected fresh feces from healthy dairy cattle reared in hokkaido, fukushima, kanagawa and okinawa prefectures between june 1996 and march 1997, and attempted to isolate vtec. the results are described below. 1) vtec was isolated from 68 (27.1%) of 251 fecal samples tested. vtec was isolated from 14 (28.0%) of 50 in hokkaido, 13 (26.0%) of 50 in fukushima, 20 (39.2%) of ... | 1999 | 10386024 |
| effect of cattle diet on escherichia coli o157:h7 acid resistance. | the duration of shedding of escherichia coli o157 isolates by hay-fed and grain-fed steers experimentally inoculated with e. coli o157:h7 was compared, as well as the acid resistance of the bacteria. the hay-fed animals shed e. coli o157 longer than the grain-fed animals, and irrespective of diet, these bacteria were equally acid resistant. feeding cattle hay may increase human infections with e. coli o157:h7. | 1999 | 10388727 |
| shiga toxin-producing escherichia coli o157 in feedlot cattle and norwegian rats from a large-scale farm. | a total of 365 faecal samples from different categories of cattle, 12 samples of untreated slurry, 50 samples of fresh droppings of feral domestic pigeons, 20 samples of fresh droppings of domestic sparrows and stool samples of 19 synanthropic rodents were examined for the presence of escherichia coli by broth enrichment culture and a subsequent immunomagnetic separation. escherichia coli o157 was found in 72 (20%) bovine samples, six (50%) samples of untreated slurry and four (40%) of 10 rats ( ... | 1999 | 10389259 |
| peroxynitrite-mediated modification of proteins at physiological carbon dioxide concentration: ph dependence of carbonyl formation, tyrosine nitration, and methionine oxidation. | the ability of peroxynitrite to modify amino acid residues in glutamine synthetase (gs) and bsa is greatly influenced by ph and co2. at physiological concentrations of co2 (1.3 mm), the generation of carbonyl groups (0.2-0.4 equivalents/subunit) is little affected by ph over the range of 7.2-9.0, but, in the absence of co2, carbonyl formation increases (from 0.1- 1.2 equivalents/subunit) as the ph is raised from 7.2 to 10.5. this increase is attributable, in part but not entirely, to the increas ... | 1999 | 10393903 |
| decay-accelerating factor and cytoskeleton redistribution pattern in hela cells infected with recombinant escherichia coli strains expressing dr family of adhesins. | escherichia coli strains expressing dr fimbriae are able to enter epithelial cells by interacting with a complement-regulatory protein, decay-accelerating factor. this model of bacterial internalization, with a well-characterized bacterial ligand and host receptor, provides a unique opportunity to investigate the early stages of invasion. we used immunofluorescence staining techniques to examine the distribution of receptor and cytoskeletal proteins in hela cells infected with e. coli recombinan ... | 1999 | 10417165 |
| purification and properties of proline-rich antimicrobial peptides from sheep and goat leukocytes. | we purified three proline-rich antimicrobial peptides from elastase-treated extracts of sheep and goat leukocytes and subjected two of them, oabac5alpha and chbac5, to detailed analysis. oabac5alpha and chbac5 were homologous to each other and to bovine bac5. both exhibited potent, broad-spectrum antimicrobial activity under low-concentration salt conditions. while the peptides remained active against escherichia coli, pseudomonas aeruginosa, bacillus subtilis, and listeria monocytogenes in 100 ... | 1999 | 10417180 |
| acute phase response in dairy cows with experimentally induced escherichia coli mastitis. | six finnish ayrshire cows were challenged intramammarily with 1500 cfu of escherichia coli (e. coli) into single udder quarters, and the challenge was repeated into contralateral quarters 3 weeks later. all cows received flunixine meglumine once, and 3 of them were also treated with enrofloxacin. at the 2nd challenge, treatments were changed vice versa. the development of mastitis was followed by monitoring of systemic and local clinical signs, and with serial milk and serum samples. intramammar ... | 1999 | 10418194 |
| isolation of verocytotoxin-producing escherichia coli o157:h7 from cattle at slaughter in italy. | cattle arriving for slaughter at a large abattoir in northern italy between april 1997 and january 1998 were examined for intestinal carriage of verocytotoxin-producing escherichia coli (vtec) o157 using an immunomagnetic separation technique. sixty sorbitol non-fermenting vtec o157 strains were isolated from 59 (13.1%) of the 450 cattle examined. in particular, vtec o157 was found in 37 (16.6%) of 223 feedlot cattle and in 22 (16.1%) of 137 dairy cull cows, but not in the 90 veal calves sampled ... | 1999 | 10418874 |
| factors associated with fecal shedding of verotoxin-producing escherichia coli o157 on dairy farms. | fecal samples were collected from 4,361 dairy cows on 91 dairy operations between 26 february and 8 july 1996. fecal samples were cultured for escherichia coli o157, and positive isolates were probed for verotoxin-producing genes. a total of 52 (1.2%) fecal samples on 22 (24.2%) operations were positive for verotoxin-producing e. coli o157. herds in which samples were collected on or after 1 may 1996 were significantly more likely to test positive than herds sampled before that date (odds ratio ... | 1999 | 10419200 |
| antibacterial effect of lactoferricin b on escherichia coli o157:h7 in ground beef. | the antibacterial activity of lactoferricin b on enterohemorrhagic escherichia coli o157:h7 in 1% peptone medium and ground beef was studied at 4 and 10 degrees c. in 1% peptone medium, 50 and 100 microg of lactoferricin b per ml reduced e. coli o157:h7 populations by approximately 0.7 and 2.0 log cfu/ml, respectively. studies comparing the antibacterial effect of lactoferricin b on e. coli o157:h7 in 1% peptone at ph 5.5 and 7.2 did not reveal any significant difference (p > 0.5) at the two ph ... | 1999 | 10419266 |
| excretion of vtec o157 by cattle. | 1999 | 10420488 |