Publications

the uncoating and infectivity of the flavivirus west nile on interaction with cells: effects of ph and ammonium chloride.	infectivity of the west nile virus (wnv; flaviviridae) was inactivated on exposure for brief periods (90 s) to ph 6.6 and below. this inactivation was not due to decreased interaction between cells and acid-treated virus. the rna of [3h]uridine-labelled virus particles prebound to the cell surface before acidic ph treatment underwent rapid uncoating within 1 min at 37 degrees c at the same ph values that inactivated virus particles. the uncoating of [3h]uridine-labelled virus particles was also ...	1986	3746254
the uncoating and infectivity of the flavivirus west nile on interaction with cells: effects of ph and ammonium chloride.	infectivity of the west nile virus (wnv; flaviviridae) was inactivated on exposure for brief periods (90 s) to ph 6.6 and below. this inactivation was not due to decreased interaction between cells and acid-treated virus. the rna of [3h]uridine-labelled virus particles prebound to the cell surface before acidic ph treatment underwent rapid uncoating within 1 min at 37 degrees c at the same ph values that inactivated virus particles. the uncoating of [3h]uridine-labelled virus particles was also ...	1986	3746254
antibacterial immunity to vibrio cholerae in rats.	blind loops prepared in the small intestines of fasted, mgso4-treated rats were shown to provide a simple, consistent and inexpensive means of studying mucosal colonisation by vibrio cholerae serogroup o1. when c. 2000 cfu were injected, the number of mucosa-associated v. cholerae in each loop increased by c. 5-6 orders of magnitude in 10-14 h, without enterotoxin-induced fluid production. scanning electronmicroscopy and culture suggested that most surface-associated organisms were present in th ...	1986	3746875
naloxone antagonizes the hyperdipsic effect of sulpiride in a salt-preference test in male and female rats.	the consumption of saline and water in a two-bottle test of salt-preference was measured after the administration of sulpiride, a dopamine receptor antagonist and of naloxone, an antagonist at opiate receptors. the two drugs were injected alone, or in combination. three concentrations of saline (0.125, 0.6 and 1.7% nacl solutions) were used and the tests were carried out using both male and female, water-deprived rats. when the rats were allowed the choice between a highly-preferred 0.125% nacl ...	1986	3748320
[a typhoid vaccine dried by direct vacuum desiccation].		1986	3749725
isolation and identification of prostaglandins of the e and f series in testes and semen of lowland-black-white breed of bulls.	the prostaglandins of e and f series were obtained from testes and semen of sexually mature bulls of lowland-black-white breed. from 1 g of fresh testes tissue we obtained 7.01 x 10(-9) m prostaglandin of the f series (pgf) and 20.65 x 10(-9) m prostaglandin of the e series (pge): from 11 of semen 3.28 x 10(-6) m of pgf and 10.58 x 10(-6) m of pge were obtained as well. the prostaglandins thus obtained displayed biological activity in experiments on the isolated small intestine of the rabbit.	1986	3751615
modulation of endothelium-dependent responses by chronic alterations of blood flow.	to determine whether the blood flow and o2 tension to which a blood vessel is chronically exposed could modulate endothelium-dependent responses, these parameters were altered in the dog either by causing partial occlusion of the femoral artery or by creating a fistula between the femoral artery and vein. blood flow was reduced by 75% in the clamped artery; mean arterial pressure was unchanged. in the vessels proximal to the fistula, blood flow was elevated and o2 tensions were similar in the ve ...	1986	3752266
effects of microcosm salinity and organic substrate concentration on production of vibrio cholerae enterotoxin.	the effects of aquatic processes on production of cholera toxin by vibrio cholerae were studied with seawater microcosms. several salinity and organic nutrient concentrations were employed. at 10 g of organic nutrient per liter of seawater, toxin production increased as the salinity was increased. at lower organic nutrient concentrations, toxin production was markedly enhanced when the salinity was 20 and 25%. toxin concentration increased with salinity, independent of cell concentration and tox ...	1986	3752994
a reappraisal of the complement fixation test using soluble mycobacterium avium antigen for the detection of m. paratuberculosis infection in cattle.	serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to mycobacterium paratuberculosis with a complement fixation test (cft) employing a heat extracted, soluble m. avium antigen. microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease-free. the specificity and sensitivity of the cft was 92.3% and 74.4% respectively. phenol treatment of serums before testing w ...	1986	3753338
is reactive gliosis a property of a distinct subpopulation of astrocytes?	we have shown previously that the a2b5 monoclonal antibody distinguishes two types of glial fibrillary acidic protein-containing astrocytes in semithin frozen sections of adult rat optic nerve: a2b5- (type-1) astrocytes are found mainly at the periphery of the nerve, where they form the glial limiting membrane, while a2b5+ (type-2) astrocytes are found mainly in the interior of the nerve and constitute more than 65% of the astrocytes in the adult optic nerve. in the present study we show that al ...	1986	3753719
the effect of arylazido aminopropionyl atp (anapp3) on inhibition of pelvic nerve evoked contractions of the cat urinary bladder.	previously reported work has provided evidence that arylazido aminopropionyl atp (anapp3), a p2-receptor antagonist, blocks the effects of atp and other purine analogs, in the urinary bladder of the cat. this antagonism appeared limited to those effects mediated by p2-receptors, such as contraction of the urinary bladder. however, it was noted that anapp3 did alter the effects of adenosine, mediated through p1-receptors, in some preliminary experiments. a series of experiments was undertaken to ...	1986	3753940
sensitive determination of tyrosine metabolites, p-hydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenyl-acetic acid and 4-hydroxy-3-methoxymandelic acid, by gas chromatography-negative-ion chemical-ionization mass spectrometry. application to a stable isotope-labelled tracer experiment to investigate their metabolism in man.	a method has been established for studying the dynamic metabolism of tyrosine to its metabolites in humans using a deuterium-labelled amino acid. phenylalanine-d5 was administered orally to human subjects (5 mg/kg) and the levels of p-hydroxyphenylacetic acid-d4, 4-hydroxy-3-methoxyphenylacetic acid-d3, and 4-hydroxy-3-methoxymandelic acid-d3 excreted into urine every hour were determined by gas chromatography-negative-ion chemical-ionization mass spectrometry. this method was also applied to so ...	1986	3753983
proteolytic modification of rat prolactin by subcellular fractions of the lactating rat mammary gland.	the current study explored prolactin proteolysis by rat lactating mammary gland. 125i-labelled rat prolactin was incubated with tissue fractions of lactating mammary gland and the extent of prolactin degradation and fragment formation was visualized and densitometrically quantitated from autoradiographs derived from sds-polyacrylamide gel electrophoresis under reducing conditions. at ph 4.5, the 25 000 x g pellet of mammary gland converted intact prolactin (23 kda band) to proteolytic fragments ...	1986	3754158
the binding of pirenzepine to digitonin-solubilized muscarinic acetylcholine receptors from the rat myocardium.	the binding of pirenzepine to digitonin-solubilized rat myocardial muscarinic acetylcholine receptors has been examined at 4 degrees c. solubilization produced only small changes in the binding of n-methylscopolamine and atropine. in contrast to the low affinity binding of pirenzepine found to be present in in the membranes, high affinity binding was detected in the soluble preparation. in both preparations, pirenzepine binding was complex. high affinity pirenzepine binding (kd approximately 3 x ...	1986	3754173
the chemical and enzymatic oxidation of hematohemin ix. identification of hematobiliverdin ix alpha as the sole product of the enzymatic oxidation.	oxidative cleavage of hematohemin ix in pyridine solution in the presence of ascorbic acid (coupled oxidation), followed by esterification of the products with boron trifluoride/methanol produced the four possible hematobiliverdin dimethyl esters in 11.1% overall yield. transetherifications took place simultaneously with the esterification reaction and resulted in the formation of the dimethyl ester of hematobiliverdin ix gamma 8a,13a-dimethyl ether (1.8%), the dimethyl ester of hematobiliverdin ...	1986	3754208
morphometric analysis and topological organization of nuclear matrix in freeze-fractured electron microscopy.	the ultrastructural organization of nuclear matrix, purified from intact or membrane-denuded rat liver nuclei, has been analysed by means of freeze-fracturing technique. this method avoids dehydration and embedding which, in conventional thin sectioning, partly distort or mask the matrix ultrastructure. the various matrix components, and mainly the peripheral lamina and the inner network revealed complex arrangements undetectable with conventional techniques. morphometric analyses performed with ...	1986	3754218
effects of isaxonine phosphate and analogs on fibroblast metabolism in culture.	human skin fibroblasts in confluent cultures were incubated for 24 h in the presence of isaxonine phosphate (nerfactor) and several related factors. the incorporation of 14c-proline into secreted proteins and the release of collagen into the medium were inhibited. when the cells were incubated for an additional period of 24 h after thorough washing, protein and collagen syntheses were found to be identical to those of controls, demonstrating that the inhibition of protein synthesis was independe ...	1986	3754223
three dimensional structure of the leishmania amastigote as revealed by computer-aided reconstruction from serial sections.	computer-aided reconstruction from serial sections has been used to analyse the 3-dimensional structure of entire amastigotes of leishmania mexicana and to determine the number, arrangement and volume of each organelle. in two reconstructions, the lysosome-like 'megasomes' were the most numerous organelle, there being 34 in one amastigote, and they comprised as much as 15% of the total cell volume. in contrast, as few as 9 glycosomes were present, accounting for less than 1% of the cell volume. ...	1986	3754324
[toxicologic studies on irradiated feed in pigs].	toxicological investigations in experimental animals given irradiated feed and studies on the possible transfer of toxic factors from this feed to the animal are reviewed. irradiated feed did not affect reproduction of rats and pigs. extensive toxicity studies in pigs continued over a sixteen-week period and rats (2 1/2 years) did not reveal any changes which were attributable to irradiation to the feed.	1986	3754356
cartilage-inducing factor-a. apparent identity to transforming growth factor-beta.	comparison of the sequence of the n-terminal 30 amino acids of cartilage-inducing factor-a (cif-a) from bovine demineralized bone with the corresponding sequence of human transforming growth factor-beta (tgf-beta) revealed 100% identity. furthermore, cif-a stimulated normal rat kidney fibroblasts to become anchorage-independent and form colonies in soft agar (in the presence of epidermal growth factor) in a manner similar to tgf-beta. similarly, tgf-beta from human platelets induced rat muscle m ...	1986	3754555
a model for computer-aided analysis of biomechanical properties of the plantaris longus tendon in the rabbit.	based on the present rapid development of user-friendly computer programs for non-linear curve fitting, the function p = a1 epsilon + a2 epsilon 3 (p = load, epsilon = strain, a1 = linear parameter, a2 = cubic parameter) for biomechanical analysis of the plantaris longus tendon in the rabbit is presented. a total of 188 plantaris longus tendons with different biomechanical properties (plin ranging from 8.73-306.2 n) achieved by different treatments were used. with a simple load-strain sequence u ...	1986	3754556
studies on the mammary tumor-inhibiting effects of diethylstilbestrol and its mono- and diphosphate.	diethylstilbestrol (des), diethylstilbestrol monophosphate (des-mp) and diethylstilbestrol diphosphate (des-dp) were tested for their estrogen receptor affinity, estrogenic potency and mammary tumor-inhibiting activity in vitro and in vivo. des had a much higher receptor binding affinity than its mono- or diphosphate. all three compounds inhibited the growth of the hormone-dependent mcf-7 and hormone-independent mda-mb 231 breast cancer line only at relatively high concentrations. the estrogenic ...	1986	3754557
simple immunochromometric assay for protein c activity.	protein c binds readily from human plasma to antibody-coated wells, where it may be quantitated with an iodine 125-labeled antibody to protein c. treatment with thrombin results in a small reduction in the protein c antigen detectable by this immunoradiometric assay (irma). however, activated protein c resulting from thrombin treatment and retained by the antibody on a solid phase may be detected by an overnight incubation with chromogenic substrates s-2366 or cbs 34.47. the immunochromometric a ...	1986	3754567
[3h]pirenzepine and (-)-[3h]quinuclidinyl benzilate binding to rat cerebral cortical and cardiac muscarinic cholinergic sites. ii. characterization and regulation of antagonist binding to putative muscarinic subtypes.	studies show [3h]pz identified selectively a subpopulation of muscarinic binding sites compared to classical antagonists like (-)-[3h]qnb in many central and peripheral tissues. we characterized the binding and regulation of selected antagonists to high-affinity [3h]pz (putative m1) and low-affinity pz (putative m2) sites in rat cerebral cortex (predominantly m1) and heart (predominantly m2). saturation isotherms of [3h]pz and (-)-[3h]qnb were performed under various conditions. guanyl-5'-yl-imi ...	1986	3754581
protective effect of 3-beta-hydroxy-2,3-dihydrowithanolide f against ccl4-induced hepatotoxicity.		1986	3754641
cadmium toxicity in kidney cells. resistance induced by short term pretreatment in vitro and in vivo.	epithelial cells from the kidney were freshly isolated from rats pretreated by daily subcutaneous doses of cdcl2 in vivo (0.5-2 mg cd/kg x 5). such cells were incubated in vitro in media with different concentrations of cadmium chloride (0-200 micrograms cd/ml). there was no inhibition of cell growth in such cells. however, in cells isolated from non-treated rats, in vitro exposure to the same concentrations of cdcl2 caused a dose dependent decrease in viability. when cells, isolated from non-tr ...	1986	3754681
effect of alterations in lipid packing order by hydrophobic solutes on the association state of protein assemblies in model membranes.	the effects of two hydrophobic solutes which perturb lipid packing order, permethrin and allethrin, on the aggregated state of a lipid membrane-incorporated protein, bacteriorhodopsin (br), have been determined by resonance energy transfer measurements. as temperature is increased from well below the main gel-fluid phase transition temperature (tc) of the lipid, patches of aggregated br dissociate into monomers, a few degrees below the tc (m.p. heyn, a. blume, m. rehorek and n.a. dencher, bioche ...	1986	3754778
hydrophobic surfactant-associated protein in whole lung surfactant and its importance for biophysical activity in lung surfactant extracts used for replacement therapy.	hydrophobic protein of 6,000 and 14,000 daltons was isolated from mammalian pulmonary surfactant obtained from canine, human, and bovine alveolar lavage material. low molecular weight, hydrophobic, surfactant-associated protein (sap), herein referred to as sap 6-14, was distinguished from sap-35, the major glycoprotein in mammalian surfactants (the 35,000 dalton glycoprotein a or apolipoprotein a) by amino acid composition, peptide mapping, and by resistance of sap 6-14 to digestion by endoglyco ...	1986	3754957
chromosomal localization of human and rat a alpha, b beta, and gamma fibrinogen genes by in situ hybridization.	in situ hybridization of radiolabeled fibrinogen cdnas to human and rat metaphase chromosomes has shown that the genes encoding the a alpha, b beta, and gamma fibrinogen subunits are syntenic in both species. our data localize the human fibrinogen gene cluster to band q31 on chromosome 4, thereby confirming and extending previous map assignments of these genes in man. we have also assigned these genes to the q31----q34 region of rat chromosome 2. this is the first map assignment of these genes i ...	1986	3755095
stabilization of intracellular recordings in the cat spinal cord using high-frequency ventilation.	stabilization of intracellular recordings by a substantial reduction of respiratory motion may be accomplished by the use of high-frequency ventilation (600-800 breaths/min). the technique is a simple one and enables maintenance of relatively constant blood gas tensions. although the improved stability was demonstrated in experiments performed on alpha motoneurones in the thoracic spinal cord, it is anticipated that a reduced respiratory movement should benefit recordings in other spinal and sup ...	1986	3756629
systemic aspergillosis as cause of myocardial infarction.	systemic aspergillosis is encountered with increasing prevalence in immunocompromised patients undergoing chemotherapy. the current communication describes the clinical and postmortem findings in three leukemic patients who developed myocardial infarction secondary to aspergillus embolization of the coronary arteries. they were all immunosuppressed owing to previous chemotherapy and had been treated for suspected fungal infection with amphotericin b (0.6 mg/kg) for at least 1 week prior to this ...	1986	3756830
concanavalin a-binding glycoproteins in the subcommissural and the pineal organ of the sheep (ovis aries). a fluorescence-microscopic and electrophoretic study.	glycoproteins rich in mannosyl or glucosyl residues were analyzed in the subcommissural organ (sco) and the pineal organ of the sheep (ovis aries). by use of concanavalin a labelled with fluorescein isothiocyanate, fluorescent material was found both in ependymal and hypendymal cells of the sco. in the pineal organ, either isolated or grouped parenchymal cells showed a marked fluorescence. these cells may correspond to ependymal elements also called "interstitial cells" or "supporting cells". in ...	1986	3757020
quantitative autoradiographic localization of the dopamine transport complex in the rat brain: use of a highly selective radioligand: [3h]gbr 12935.		1986	3758162
intracranial hypertension and cerebrospinal fluid production in dogs: effects of furosemide.	a method using chronically prepared, anesthetized dogs was devised for studying the effects of treatments of intracranial hypertension induced by applying a reversible extradural mass lesion while simultaneously measuring production of cerebrospinal fluid. this was measured with a ventricular-cisternal perfusion technique in which the rate of cisternal outflow could be controlled by a pump and matched to the inflow, allowing intracranial pressure to fluctuate despite simultaneous measurement of ...	1986	3758288
colonization & protection in infant rabbits by a choleragenoid producing strain of vibrio cholerae.		1986	3759164
local cerebral glucose utilization during intracerebral ph changes.	the effect of ventriculocisternal perfusion with mock csf with alkaline or acidic ph on the local cmrglu (lcmrglu) in the caudatoputamen was studied in artificially ventilated and relaxed rats. in control rats both lateral cerebral ventricles were perfused with mock csf at ph 7.4. in the experimental series one cerebral ventricle was infused with normal mock csf while the other was infused with mock csf in which the ph was decreased or increased by changing [hco-3]. lcmrglu was depressed in acid ...	1986	3760042
hydrolysis of all gangliosides, including gm1 and gm2, on thin-layer plates by vibrio cholerae neuraminidase.		1986	3760066
[an ecological study of vibrio cholerae o-1 and vibrio cholerae non o-1, with special regard to behavior in foodstuffs and living shellfish].		1986	3760126
the effect of c1 spinal cord transection or bilateral adrenal vein ligation on thioridazine-induced arrhythmia and death in the cat.	the phenothiazine thioridazine 1 mg/kg/min was infused intravenously into three groups of cats: (1) thioridazine alone (n = 5), (2) after bilateral adrenal ligation (n = 4), and (3) after spinal cord section at the atlanto-occipital junction (c1; n = 6). the times to arrhythmia and death with thioridazine alone were 47.8 +/- 7.8 and 72.8 +/- 5.6 minutes respectively. after bilateral adrenal ligation, arrhythmia and death occurred at 41.1 +/- 5.2 and 53.1 +/- 5.8 minutes, respectively, which show ...	1986	3760244
[superior vena cava reconstruction with an antiplatelet agent].		1986	3760629
a diphytanyl ether analog of phosphatidylserine from a methanogenic bacterium, methanobrevibacter arboriphilus.	several ninhydrin-positive lipids were found in methanogenic bacteria and the structure of one of them, designated as pnl2 from methanobrevibacter arboriphilus, was identified as a diphytanyl ether analog of phosphatidylserine. the chromatographic behavior of the lipid on thin-layer plates and on a deae-cellulose column was identical to the ester form of phosphatidylserine. the infrared spectra showed the presence of amino, carboxyl, ether, and phosphate groups, and the absence of an ester linka ...	1986	3760709
novel transmissible factors in a non-o1 vibrio cholerae and a vibrio sp.	transmissible factors encoding production of lacunae (l factors) were demonstrated in a non-o1 vibrio cholerae and a vibrio sp. of recent environmental origin. lacunae were produced in lawns of non-o1 v. cholerae indicator strains under the same assay conditions as those where lacunae were produced by the well characterized p fertility plasmid of v. cholerae o1 and the v fertility factor found in a non-cholera vibrio strain. the origin of the lacunae produced by strains harbouring the v and l fa ...	1986	3760815
myocardial ischemia stimulates catecholamine synthesis and catabolism in the dog adrenal medulla.	the effects of regional ischemia of the myocardium upon metabolism and catabolism of catecholamines were studied in the dog adrenal medulla. regional ischemia was induced by ligating a small branch of the left anterior descending coronary artery for 1 or 24 hours. sham-operated controls were run in parallel. coronary ligation for 24 hours resulted in a decrease in the level of epinephrine and an increase in the level of dopamine in the adrenal medulla, and also resulted in an increase in the act ...	1986	3761566
[familial occurrence of intracerebral cavernous angioma].	a case of familial occurrence of intracerebral cavernous angioma is reported. the patients were a mother and her eldest son, a 48-year-old woman and a 28-year-old man, respectively. the mother, a hypovascular mass in the right frontal lobe was excised surgically, and in the son a well demarcated mass in the left temporal lobe was extirpated. pathological examination in each case revealed cavernous angioma. familial occurrence of intracerebral cavernous angioma is extremely rare. in the literatur ...	1986	3762858
examination of the subdiencephalic rat brain for sites mediating pge1-induced pyrexia.	the subdiencephalic rat brain was mapped for sites capable of mediating prostaglandin-induced pyrexia. in conscious rats, pge1, 200 ng in a volume of 1 microliter, was injected unilaterally into 412 sites between the midmesencephalon and the caudal medulla. injections into only 12 sites caused a reproducible, short-latency core temperature increase of at least 0.5 degrees c. none of these was located in the paramedian brainstem, which was considered a likely site of pge1 action because of the pr ...	1986	3763660
[human experimentation and human rights. is biomedical experimentation in children permissible?].		1986	3764145
ablation of polymers and biological tissue by ultraviolet lasers.	when pulsed, ultraviolet laser radiation falls on the surface of an organic polymer or biological tissue, the material at the surface is spontaneously etched away to a depth of 0.1 to several micrometers. in the process, the depth of etching is controlled by the width of the pulse and the fluence of the laser, and there is no detectable thermal damage to the substrate. the material that is removed by etching consists of products ranging from atoms to small fragments of the polymer. they are ejec ...	1986	3764428
clinical and in vitro antiproliferative properties of recombinant dna-derived human interferon-alpha 2.	the properties of the recombinant dna-derived human leukocyte interferon, huifn alpha 2, were studied in patients with advanced leukemia or lymphoma. in vitro, huifn alpha 2 induced an increased activity of 2-5a synthetase in leukemic and in control cells indicating cellular responsiveness to ifn. huifn alpha 2 also produced a dose-responsive decline in marrow leukemia blast progenitor colony growth, and in normal hematopoietic colony formation in vitro, confirming its antiproliferative effect. ...	1986	3766908
colonization of the gut of the blue crab (callinectes sapidus) by vibrio cholerae.	attachment of vibrio cholerae to the mucosal surface of the intestine is considered to be an important virulence characteristic. vibrio cholerae, an autochthonous member of brackish water and estuarine bacterial communities, also attaches to crustacea, a significant factor in multiplication and survival of v. cholerae in nature. the ability of v. cholerae to attach to the gut wall of the blue crab (callinectes sapidus) was examined, and attachment was observed only in the hindgut and not the mid ...	1986	3767362
[activation of calcium transport in synaptosomes by phosphatidylinositol-specific phospholipase c].	the isotope labeling method was used to study the influence of phospholipases c of different origin and specificity on ca2+ accumulation in rat brain synaptosomes. it was found that phospholipases c specific to phosphatidylinositides (pi) stimulate ca2+ transport into synaptosomes, while non-specific phospholipase c, which hydrolyzes different membrane lipid fractions, decreases the ca2+ content in synaptosomes. it is supposed that the stimulating effect of pi-specific phospholipases c is determ ...	1986	3768437
relative contribution of brain and peripheral connections to postnatal growth and cell accretion in the rat olfactory bulb.	total weight and dna (cell number) determination in whole olfactory bulbs (ob) revealed that unilateral transection of the olfactory peduncle in newborn rats causes 2 x more (40%) deficit in cell number in the operated ob than section of the olfactory nerve. the deficit due to combined operations ('isolation' of ob) were additive. similar effects were observed on weight gain. it is inferred that both olfactory nerve and sources in the brain exert marked influences on postnatal cell accretion and ...	1986	3768730
further analysis of multiple forms of rabbit hepatic glutathione s-transferase.		1986	3769093
variations in cellular polyamine compositions and contents of vibrio species during growth in media with various nacl concentrations.		1986	3769106
[antibiotic resistance of strains of vibrio cholerae eltor isolated in douala (cameroon)].	endemic cholera has been prevalent in douala since 1972, with sudden epidemic outbreaks occurring every two years during the dry season. the massive and systematic use of chemoprophylaxis since april, 1983 has led to the selection of strains of vibrio cholerae eltor that are resistant to sulphamide and tetracycline. during the 1984-1985 epidemic, 89.3% of the isolated strains were resistant to sulphamides, 87.5% to a sulfamethoxazole-trimethoprim combination and to the 0/129 disk, 55.3% to tetra ...	1986	3769118
preservation of donor heart function and high-energy stores by continuous perfusion with synthetic plasma at 22 degrees c.	we evaluated high-energy phosphate (hep) levels and the ability to perform work in rat hearts preserved by standard techniques (0.9% nacl arrest and storage at 4 degrees c) and by continuous coronary perfusion at 22 degrees c, ph 7.25, and 55 mm hg for 4 or 8 hr with krebs-henseleit buffer (khb), modified morgan's solution (mcs), or a medium developed in our lab (physiologically complete medium or pcm). cardiac work was evaluated in the rewarmed hearts with use of a working heart preparation at ...	1986	3769190
preservation of donor heart function and high-energy stores by continuous perfusion with synthetic plasma at 22 degrees c.	we evaluated high-energy phosphate (hep) levels and the ability to perform work in rat hearts preserved by standard techniques (0.9% nacl arrest and storage at 4 degrees c) and by continuous coronary perfusion at 22 degrees c, ph 7.25, and 55 mm hg for 4 or 8 hr with krebs-henseleit buffer (khb), modified morgan's solution (mcs), or a medium developed in our lab (physiologically complete medium or pcm). cardiac work was evaluated in the rewarmed hearts with use of a working heart preparation at ...	1986	3769190
electron microscopy of the parameres formed by the centromeric heterochromatin of human chromosome 9 at pachytene.	the structure and arrangement of the parameres, which are small bodies representing part of the heterochromatin of human chromosome 9 at pachytene, were studied using transmission and scanning electron microscopy. parameres appear to be denser than other parts of the chromosomes but have a similar fibrous substructure. the most common arrangement is clusters on the axis of the bivalent, consisting of varying numbers of parameres of variable size. the parameres are joined to each other and to the ...	1986	3769651
[co-localization of ribosomes and elongation factor 2 in the cytoplasm of mouse embryonal fibroblasts].		1986	3769751
serological characterization of humoral lectin from heterometrus granulomanus scorpion hemolymph.	a lectin in the hemolymph of indian scorpion heterometrus granulomanus was detected by agglutination of human and animal erythrocytes. the agglutinating activity was enhanced in presence of ca2+ ion. the lectin shows specificity for sialic acid like many other sialic acid-specific lectins such as "limulin", as the agglutination of erythrocytes was completely abolished by treatment with vibrio cholerae neuraminidase. however, neuraminidase-treated rat and mouse erythrocytes exhibited high titers. ...	1986	3770265
collagen binding to staphylococcus aureus.	staphylococcus aureus can bind soluble collagen in a specific, saturable manner. we have previously shown that some variability exists in the degree of collagen binding between different strains of heat-killed, formaldehyde-fixed s. aureus which are commercially available as immunologic reagents. the present study demonstrates that live s. aureus of the cowan 1 strain binds amounts of collagen per organism equivalent to those demonstrated previously in heat-killed, formaldehyde-fixed bacteria bu ...	1986	3770947
oligodeoxynucleotide stability in subcellular extracts and culture media.	oligodeoxynucleotide degradation was studied in four systems in order to assess the importance of degradation in hybridization arrest experiments dependent on oligodeoxynucleotides complementary to mrna sequences. oligodeoxynucleotides were not detectably degraded over 2 h at 37 degrees c in rabbit reticulocyte lysate or dulbecco's modified essential medium with 5% fetal calf serum, but were degraded over 2 h in hela cell postmitochondrial cytoplasmic extract, and were degraded within 15 min in ...	1986	3772027
binding and mitogenic properties of a galactosyl-specific lectin from the tunicate didemnum candidum for murine thymocytes and splenocytes.	the affinity-purified major galactosyl-specific lectin (dcl-i) from the tunicate didemnum candidum binds and induces blastogenesis in untreated and vibrio cholerae neuraminidase (vcn)-treated murine splenocytes. however, it only binds and stimulates murine thymocytes when they have been pretreated with vcn. binding and stimulation of vcn-treated splenocytes and thymocytes is specifically inhibited by d-galactose. binding of lectin to splenocytes is highly increased by the vcn treatment, but the ...	1986	3772103
pulmonary biochemical effects of inhaled phosgene in rats.	three exposure regimens were used to study the time course of indicators of lung damage and recovery response to single or repeated exposures to phosgene (cocl2). rats were sacrificed immediately or throughout a 38-d recovery period after inhalation of 1 ppm cocl2 for 4 h, at intervals during a 7-h exposure to 1 ppm phosgene, or at several time points throughout a 17-d exposure to 0.125 and 0.25 ppm cocl2 (4 h/d, 5 d/wk) and during a 21-d recovery period. regimen 1 revealed significantly elevate ...	1986	3772987
laser-assisted vasal anastomosis in the rat and man.	in order to study the efficacy of laser-assisted vasal anastomosis (lava), a microscopic carbon dioxide surgical laser (xanar) was utilized in the anastomoses of human vas deferens in vitro and the sprague-dawley rat vas deferens in vivo. the longitudinal tensile and internal hydrostatic pressure strengths of laser-assisted vasal anastomoses were compared to conventional microsurgical suture anastomoses in the human vas deferens. the lava group had a greater mean internal hydrostatic pressure st ...	1986	3773082
[the characterization of cross-reacting protein antigens in gram-negative bacilli].		1986	3773265
nerve branching and terminal arborizations in the carotid body of the cat. a light microscopic study following anterograde injury filling of carotid nerve axons with horseradish peroxidase.	the terminal arborizations of carotid nerve axons within the carotid body of the cat were densely filled with horseradish peroxidase and studied under the light microscope. two types of terminal arborizations were found in contact with glomus (type i) cells. the axons differed principally in the wealth of terminal swellings. the largest and most numerous type of arborization consisted of one to several clusters of terminals of variable size and shape arising from a single fiber and distributed i ...	1986	3774156
[human response to vibration. an ergonomic applicability of current knowledge to the automobile].	with technological progress, vibrations as a risk factor for health have now extended beyond the boundaries of the workplace to become a social rather than an occupational risk. now that we know the local or general damage vibrations can cause, ergonomists and occupational health officers are increasingly involved in the preparation of techniques for the application of their knowledge to preventive measures. apparently, negative reactions derive form the impact on sensorial structures that devel ...	1986	3774194
a human y-chromosome specific repeated dna family (dyz1) consists of a tandem array of pentanucleotides.	we have determined the complete nucleotide sequence of a 3564 bp ecori fragment which represents a major component of the human y-chromosome specific repeated dna family (dyz1). sequencing result showed a tandem array of pentanucleotides after five nucleotides were inserted or deleted at four positions. 229 out of the 713 pentanucleotides were ttcca, and 297 were its single-base substituents. southern hybridization analyses of male genomic dnas showed that several endonuclease cleavage sites wer ...	1986	3774538
high chronic ambient temperature stress effects on broiler acid-base balance and their response to supplemental ammonium chloride, potassium chloride, and potassium carbonate.	four experiments were conducted to evaluate the effect of heat stress on acid-base status and the birds' response to supplemental kcl and kco3. the corn-soybean meal fish-meal basal ration (.73% k+) contained more k+ and cl- than is recommended by the national academy of sciences for chicks reared under near optimal conditions (24 c and 55% relative humidity). chicks reared under continuous thermostress (35 c, 70% relative humidity) exhibited panting phase blood alkalosis (ph of 7.46). supplemen ...	1986	3774744
follow-up study of patients with vibration syndrome in japan.	this study was performed on 187 patients with vibration syndrome treated and followed for more than five years in 10 hospitals in japan. the subjects had disturbances of circulation and sensation in their fingers and of joint movements in their upper extremities. most of the patients received treatment combining physical and drug therapy. data were analyzed to determine the effective treatment period for improving the disturbances. the blanching attack raynaud's phenomenon) and abnormal cold and ...	1986	3775315
endosulfan induced inhibition of 3h-5-hydroxytryptamine uptake in platelets.	endosulfan, a chlorinated pesticide, is widely used to control various insect pests. rats exposed to 1 mg and 3 mg endosulfan/kg for periods of 10, 30, and 60 days showed significant (p less than 0.05) inhibition of [3h]5-hydroxytryptamine (5-ht) uptake by platelet-rich plasma (prp) in an ex vivo study. rats treated with endosulfan (1 and 3 mg/kg) up to 60 days elucidated a marked inhibition of adp-induced aggregatory responses of the platelets. incubation of prp with 10 microm and 100 microm en ...	1986	3775804
[a case of synchronous contralateral renal cell carcinoma and ureteral transitional cell carcinoma].	a case of synchronous contralateral renal cell carcinoma and ureteral transitional cell carcinoma is reported. a 56-year-old man, who had been incidentally found to have an abnormal mass in the upper pole of the right kidney on ultrasound sonography, was admitted on january 8, 1985. ct scanning and renal arteriography revealed right renal malignancy. right radical nephrectomy was performed and histological examination showed adenocarcinoma, granular cell type of the right kidney. he was discharg ...	1986	3776765
mechanisms contributing to the anemia associated with a localized solid tumor.	the anemia associated with a localized solid tumor is a chronic hypoproliferative process referred to as the anemia of chronic disease. several mechanisms responsible for this anemia have been proposed. a defect in iron reutilization was well accepted until challenged by a report in 1977. evidence also exists to support bone marrow failure as a contributing factor. using wistar rats made anemic by implantation of walker-256 carcinosarcoma cells, both possibilities were investigated. by injecting ...	1986	3777012
cytoplasmic calcium in individual proximal tubular cells in culture.	the development of high quantal yield ca2+-sensitive fluorescent indicators has made microspectrofluorometric monitoring of cytoplasmic calcium feasible. the detailed technique to monitor cytoplasmic calcium concentration in individual proximal tubular cells grown on glass cover slips is described. manipulations of cytoplasmic calcium concentration by means of a ca2+ ionophore or ca2+-free medium resulted in corresponding changes of fura-2 fluorescence. parathyroid hormone elicited a fivefold in ...	1986	3777189
phospholipid ester-linked fatty acid profile changes during nutrient deprivation of vibrio cholerae: increases in the trans/cis ratio and proportions of cyclopropyl fatty acids.	the phospholipid ester-linked fatty acids of 0-day-, 7-day-, and 30-day-starved cultures of vibrio cholerae were compared. statistically significant trends were noted in the fatty acid profiles as the cells starved. the amount of the cis-monoenoic fatty acids declined (e.g., 16:1 omega 7c: 0 day, 39%; 7 day, 18%; 30 day, 11%). in contrast, the saturated fatty acids, the cyclopropyl derivatives of the cis-monoenoic fatty acids, and trans-monoenoic fatty acids increased during starvation. for inst ...	1986	3777927
behavior of vibrio cholerae in hot foods.	four food types held hot at 45 to 60 degrees c were deliberately contaminated with o1 and non-o1 vibrio cholerae strains. these organisms were assayed for survival and recovery from the foods within 1 h of the time the food was kept hot. the results showed no growth of v. cholerae non-o1 on thiosulfate-citrate bile-sucrose agar plates after 24 h of incubation at 37 degrees c for food held hot at 50 to 60 degrees c. growth was low for v. cholerae o1 and was not achieved in some instances in which ...	1986	3777928
comparative study of expression of hemagglutinins, hemolysins, and enterotoxins by clinical and environmental isolates of non-o1 vibrio cholerae in relation to their enteropathogenicity.	a comparative study was undertaken of clinical and environmental isolates of non-o1 vibrio cholerae with respect to their hemagglutinating, hemolytic, enterotoxigenic, and enteropathogenic activities. cell-associated hemagglutinin titers of the clinical and environmental isolates did not differ much, although the clinical isolates displayed higher cell-free hemagglutinin titers compared with those of environmental isolates. culture supernatants of 61.5% (24 of 39) of clinical isolates showed hem ...	1986	3777932
[intraperitoneal cavity injection of ok-432 with fresh human serum in a case of carcinomatous peritonitis--determination on the complement-derived chemotactic factor].	large numbers of neutrophils appear in the peritoneal cavity within a few hours after administration of ok-432 into the peritoneal cavity, and play an important role in the destruction of cancer cells. as ok-432 can activate complement in vitro, it is possible that a chemotactic factor such as c5a is generated and attracts neutrophils into the cavity. we injected ok-432 with fresh human serum preincubated at 37 degrees c for 60 min in order to generate large amounts of complement-derived chemota ...	1986	3777961
early penetration of human sperm through the vestments of human eggs in vitro.	penetration of human sperm through the vestments of human oocytes during the first 3 h after insemination was investigated to determine the time taken for sperm capacitation, which precedes the acrosome reaction and fertilization. twelve oocytes from women who became pregnant by ivf, recovered at laparoscopy after appropriate stimulation, were examined by electron microscopy. follicular development was controlled by administration of clomiphene citrate, hmg, and hcg. oocytes were cultured in whi ...	1986	3778016
vibrio cholerae non-01 cellulitis.	a 46-year-old man presented with pain and fever and a postphlebitic ulcer on his left leg. the wound was suppurative and open at the margins, but there was little underlying fasciitis and no apparent muscle or blood vessel involvement. three separate wound cultures were obtained at two-day intervals, and all showed only vibrio cholerae non-01. the patient was successfully treated with cefazolin sodium. this marks the second documented case of v cholerae non-01 type alone as a causative agent of ...	1986	3778149
a heat-stable enterotoxin of vibrio cholerae non-01: chemical synthesis, and biological and physicochemical properties.		1986	3779031
changes in regional myocardial perfusion by muscarinic receptor subtypes in dogs.	to determine the muscarinic receptor subtype (m1 or m2 or both) responsible for cholinergic coronary vasodilatation and alterations in the transmural distribution (endocardial to epicardial blood flow ratio) of coronary blood flow, systemic and coronary haemodynamic indices and regional myocardial blood flow (radioactive microspheres) were measured in anaesthetised dogs. submaximal vasodilative doses of the mixed muscarinic agonist acetylcholine were given by intracoronary infusion to avoid peri ...	1986	3779744
role of adenosine in mediating myocardial blood flow response to isoproterenol: observations in closed chest, sedated, domestic swine.	to test the hypothesis that adenosine is required to mediate the sustained increase in myocardial flow evoked by isoproterenol haemodynamic indices, myocardial blood flow (microspheres), and regional myocardial oxygen consumption were measured in eight closed chest, sedated pigs at control, after isoproterenol (6.9(2.8) ng x kg-1 x min-1 (mean (sd)) infused into the left anterior descending coronary artery, repeat control, and after a simultaneous infusion of the same dose of isoproterenol and a ...	1986	3779747
an evaluation of the genotoxic potential of di-isononyl adipate.	di-isononyl adipate (dina) is one of a group of adipates used primarily as plasticizers. concern over the mutagenic and carcinogenic potential of these materials was stimulated by the finding that one member of this class, di-(2-ethylhexyl) adipate (deha), induced liver tumors in female mice in a chronic feeding study. accordingly, the genotoxic potential of dina was evaluated in a battery of in vitro tests including the salmonella/mammalian microsome mutagenicity assay, the mouse lymphoma tk +/ ...	1986	3780616
non-agglutinating vibrio (non 0-1 v. cholerae) septicemia.		1986	3781609
spiral conformation of vibrio cholerae as determined by scanning electron microscopy of elongated cells induced by cephalexin treatment.	the elongated cells of vibrio spp. induced by cephalexin treatment were examined by scanning electron microscopy. the results showed that vibrio cholerae has a twisted cell body and a right-handed spiral conformation and that the cell bodies of v. parahaemolyticus and v. alginolyticus are straight rather than curved.	1986	3782045
microsporum nanum infection in hog farmers.	microsporum nanum is a dermatophyte that can cause disease in both man and animals and is most frequently associated with ringworm infection in pigs. human infections are rare in the literature. three new cases of human m. nanum infection are reported here. two patients had inflammatory tinea faciei and one had tinea cruris. the patients were successfully treated with clotrimazole or miconazole cream, sometimes combined with griseofulvin. all three patients were hog farmers and lived in the same ...	1986	3782533
comparison of two media in a human in vitro fertilization program: lack of significant differences in pregnancy rate.		1986	3783015
measurements of adipose tissue respiration in a closed chamber using an oxygen sensor: methodological considerations.	a new polarographic system for measurement of oxygen consumption in vitro has been constructed to minimize different types of errors. the system was built of polyamide-6, monax glass, stainless steel, and rubber to diminish oxygen leakage and gas capacitance properties. with these materials the system was gas-tight. nevertheless, the incubation chambers had oxygen capacitance properties that had to be corrected for mathematically. thus, in order to obtain more exact absolute values of the respir ...	1986	3783050
organochlorine pesticides in human milk from different areas of kenya 1983-1985.	residue levels of the chlorinated hydrocarbons p,p'-ddt (2,2-bis(p-chlorophenyl)-1,1,1-trichloroethane), p,p'-dde (2,2-bis(p-chlorophenyl)-1,1-dichloroethane), hexachlorobenzene (hcb), alpha-, beta-, and gamma-hexachlorocyclohexane (hch), aldrin, dieldrin, and polychlorinated biphenyls (pcbs) were determined in human milk of kenyan mothers living in different areas of kenya. the main organochlorine contaminants found in all the milk samples analyzed were p,p'-ddt and p,p'-dde. great regional dif ...	1986	3783766
mass screening in leprosy endemic areas of turkey: preliminary report.		1986	3784757
the acidosis of cholera. contributions of hyperproteinemia, lactic acidemia, and hyperphosphatemia to an increased serum anion gap.	to study the metabolic acidosis that occurs during the diarrhea of cholera, we examined the serum anion gap in 21 patients with hypovolemic shock due to vibrio cholerae infection. measurements of serum electrolytes, as well as divalent cations and the anionic contributions of serum proteins, lactate, phosphate, and serum creatinine, were made at the time of admission, after rehydration, and during convalescence. at the time of admission, the mean serum concentration of sodium was 134.8 mmol (meq ...	1986	3785323
demonstration of a plasmid-borne gene encoding a thermostable direct hemolysin in vibrio cholerae non-o1 strains.	of 15 vibrio cholerae non-o1 strains, 2 produced a hemolysin termed nag-rtdh, which is very similar to the thermostable direct hemolysin of v. parahaemolyticus. these two strains contained dna sequences which are homologous to a dna probe for the v. parahaemolyticus thermostable direct hemolysin gene. a probe-positive 9-kilobase hindiii fragment was cloned from a plasmid of a v. cholerae non-o1 strain into plasmid pbr322, and the resulting escherichia coli clones produced intracellular nag-rtdh.	1986	3789717
[studies on the enhancement of anti-tumor activity in patients with cervical cancer by local administration of ok-432].	in order to investigate an effective administration method for ok-432, a potent biological response modifier (brm), in patients with cervical cancer, preoperative local administration was conducted and the following results obtained: immunohistochemical examination using monoclonal antibodies indicated that the group given local injection of ok-432 showed a marked increase in ia antigen-positive t4 cells, ia antigen-positive macrophages and nk cells in the tissue of the area surrounding the cerv ...	1986	3789753
tumor therapy of neoplastic diseases with tumor cells and neuraminidase. further experimental studies on chessboard vaccination in canine mammary tumors.	the therapeutic effect of i.d. injection of tumor cells mixed with vibrio cholerae neuraminidase (vcn) on tumor progression in dogs with spontaneous mammary tumors was investigated. the i.d. injections were performed in a chessboard-like manner: different numbers (10(5), 10(6), 10(7), and 10(8) of mitomycin-treated autologous tumor cells (m-tc) were each mixed with different amounts (10, 50, and 100 mu) of vcn. these different mixtures were injected i.d. at different sites in dogs on the day of ...	1986	3791304
recombination aneusomy of chromosome 5 associated with multiple severe congenital malformations.	a male infant is described in whom congenital anomalies were recognized prenatally by ultrasound examination. the infant was delivered following spontaneous labor and died approximately 15 min after birth. an autopsy revealed major anomalies in the central nervous system (holoprosencephaly with premaxillary agenesis), the gastrointestinal system (esophageal atresia) and the heart (tetralogy of fallot). chromosomal studies revealed recombinant chromosome 5 [46,xy, rec(5), dup q, inv(5)(p15q32)], ...	1986	3791677
a comparative study on the cellular processing of free and gold-conjugated transferrin.	the kinetics of the transferrin-reticulocyte interaction was studied using free and colloidal gold-conjugated double labelled transferrin (tf and autf, respectively). simultaneous biochemical and morphological experiments provided the following information: 1. the cellular recycling of tf is significantly faster than that of autf. 2. autf induces a marked increase in the number of multivesicular elements (mve) in rabbit reticulocytes. 3. the release of autf from the cells is very slow and accumu ...	1986	3792344
immunoelectrophoresis in quality control of veterinary clostridial products.	each year the pharmaceutical industry produces 150-250 batches of anti-clostridial vaccines and sera for veterinary use in the u.k. under medicines act requirements these products must be tested for potency before release to the public, such tests being performed in animals. the ministry of agriculture, as licensing authority, also carries out potency test on representative samples of these products. to avoid unnecessary use of animals we employ immunoelectrophoresis (iep) as a screening test. r ...	1986	3792642
molecular cloning of a gene coding for a vibrio cholerae haemagglutinin.	recombinant plasmids encoding a vibrio cholerae haemagglutinin were isolated from the highly virulent v. cholerae strain c5 by cosmid cloning. both escherichia coli hb101 containing the recombinant plasmids and v. cholerae c5 were able to agglutinate a variety of erythrocytes from human and animal origin; this haemagglutination was not inhibited by d-mannose or l-fucose. subcloning of the recombinant cosmid dna revealed that a 1.3 kb dna fragment was sufficient for haemagglutinin production in e ...	1986	3794648
composition and immunochemical properties of the cell surface proteins of vibrio cholerae.	the composition and immunochemical properties of cell surface proteins of vibrio cholerae belonging to both the biotypes (classical and el tor) and the serotypes (ogawa and inaba) were investigated. proteins were isolated by extraction with edta/nacl. when the extract was further treated with sodium deoxycholate, a product significantly enriched with the major protein was obtained. the surface localization of these proteins was confirmed by immunoelectron microscopy using protein a-colloidal gol ...	1986	3794649