Publications

second-generation cephalosporins.		1986	3081544
enumeration of b lymphocytes.		1986	3083188
natural killer cell response to interferons.	the availability of recombinant interferons has facilitated a comparison of the in vitro effects of interferon-alpha and -gamma upon human natural killer (nk) cells. in the absence of interferon high and low nk responders exist. repeated testing of the same individuals revealed different but stable nk patterns to in vitro addition of interferon-alpha and -gamma furthermore, the nk cell response patterns differed depending on whether the interferons were administered in combination or separately. ...	1986	3084143
effects of prostaglandin synthesis inhibition on sympathetic-and parasympathetic-mediated coronary hemodynamic responses.	in chloralose-anesthetized dogs with the left circumflex coronary artery perfused at constant flow, the effects of indomethacin or naproxen on coronary and systemic responses to sympathetic and parasympathetic stimulation were evaluated. sympathetic stimulation was evoked either by 1-min carotid artery occlusion or by epinephrine (5 micrograms) or norepinephrine (5 micrograms) intracoronary administration. reflex or direct parasympathetic stimulation was produced by ouabain (40 micrograms) or ac ...	1986	3084760
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.	lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...	1986	3085110
some properties of lipoxygenase activities in cytosol and microsomal fractions of mouse epidermal homogenate.	lipoxygenase activity in microsomal fraction of mouse epidermal homogenate was characterized in comparison with cytosol lipoxygenase activity. the major activity was identified as 12-lipoxygenase in microsomal fraction as well as in cytosol fraction by the analyses with high-performance liquid chromatography and gas chromatography-mass spectrometry. apparent km values of cytosol and microsomal 12-lipoxygenase for arachidonic acid were 5.0 microm and 6.2 microm respectively. apparent vmax values ...	1986	3085110
[use of the radiosaturation analysis method in the diagnosis and monitoring of viral hepatitis].		1986	3087635
gastric form of alpha chain disease.	a case of alpha chain disease, involving the stomach only, is reported in an algerian man suffering from epigastric pains. upper digestive tract fibreoptic endoscopy showed two antral ulcers and an ulcerative gastritis pattern, which promptly disappeared with cimetidine treatment. antral biopsies at a distance from the ulcers, but not of the ulcer crater itself, disclosed a dense infiltration of antral lamina propria by mature or sometimes atypical plasma cells. on transmural surgical antral bio ...	1986	3087826
ultrastructure of cyst wall of sarcocystis of mus musculus, and reaction to cyst wall.		1986	3088138
regulation of human gamma-interferon and beta-interferon gene expression in pha-activated lymphocytes.	two interferon (ifn) messengers were synthesized in phytohemagglutinin (pha)-activated lymphocytes: ifn-gamma mrna and a messenger hybridizing with the ifn-beta 2 probe. they were induced rapidly and declined at a stage when overall rna was still efficiently transcribed. the ifn-beta 2 mrna (15s) present in the lymphocytes was slightly different from its fibroblastic counterpart (14s). the kinetics of the accumulation and decay of both lymphocyte ifn messengers differed when assessed by hybridiz ...	1986	3088147
evidence that some membrane ligands modulate the plasmalemma fluidity of endothelial cells in culture.	the effect of some membrane ligands on the plasmalemmal fluidity of endothelial cells from bovine aorta in culture was investigated. the ligands used were: cationic ferritin (pi 8.5), soybean agglutinin, concanavalin a, wheat germ agglutinin, as well as glutaraldehyde at different concentrations. the fluidity probe employed was 1,6-diphenyl-1, 3, 5-hexatriene (dph) and the parameter determined to quantify the fluidity was fluorescence steady-state anisotropy. the optimum time interval required b ...	1986	3088620
bone marrow aspirate culture superior to streptokinase clot culture and 8 ml 1:10 blood-to-broth ratio blood culture for diagnosis of typhoid fever.	we compared the sensitivities of bone marrow aspirate culture (bmac), 3 ml 1:4 and 8 ml 1:10 blood-to-broth ratio blood cultures (bc), 8 ml streptokinase clot culture (stkcc) and rectal swab culture (rsc) for isolating salmonella typhi and s. paratyphi a from 61 patients with typhoid or paratyphoid fever in jakarta, indonesia. bmac (92%) was significantly more sensitive than 8 ml bc (62%), 8 ml stkcc (51%), 3 ml bc (44%), rsc (56%) and the 19 ml combination of all three bc methods (71%). the com ...	1986	3089041
effect of thyrotropin-releasing hormone on vestibular compensation in primates.	continual administration of thyrotropin-releasing hormone (trh) (250 micrograms/kg/day) for 14 days using an osmotic mini-pump in unilateral labyrinthectomized squirrel monkeys induced accelerated vestibular compensation. decrease in total gait deviation counts and reduction of slow-phase eye velocity of spontaneous nystagmus were significantly faster in the trh-treated group as compared with the control group.	1986	3089045
characterization of cyanobacterial ferredoxin-nadp+ oxidoreductase molecular heterogeneity using chromatofocusing.	chromatofocusing has been used as an analytical tool to check preparations of the enzyme ferredoxin-nadp+ oxidoreductase (ec 1.18.1.2) purified in either the presence or absence of the serine protease inhibitor phenylmethylsulfonyl fluoride from the cyanobacterium anabaena sp. strain 7119. only one isoelectric species was found when the crude extract was processed in the presence of the protease inhibitor. nevertheless, when the inhibitor was omitted, four ionic forms of the enzyme--showing appa ...	1986	3089056
the use of galactosyltransferase to probe nitrocellulose-immobilized glycoproteins for nonreducing terminal n-acetylglucosamine residues.	we report the use of udpgalactose:n-acetyl-d-glucosaminyl-glycopeptide 4-beta-d-galactosyl-transferase (ec 2.4.1.38), purified from bovine milk, to detect nonreducing terminal n-acetylglucosamine residues on glycoproteins immobilized on nitrocellulose by electrophoretic transfer from sodium dodecyl sulfate-polyacrylamide gels. soluble galactosyltransferase incorporates radiolabeled galactose from the substrate udp-[6-3h]galactose into the appropriate immobilized acceptor with high specificity. i ...	1986	3089057
an unusual case of gardnerella vaginalis septicaemia.		1986	3089498
reconstitution of the masking effect of sialic acid groups on sialidase-treated erythrocytes by the action of sialyltransferases.	glutardialdehyde-fixed or native rat erythrocytes were partially desialylated by the action of vibrio cholerae sialidase, resulting in the binding of these cells to homologous peritoneal macrophages. resialylation of these erythrocytes by purified alpha-(2----3)- or alpha-(2----6)-sialyltransferases with cmp-n-acetylneuraminic acid led to the incorporation of 60-80% of the enzymically released sialic acid. binding of the resialylated erythrocytes to peritoneal macrophages was reduced when compar ...	1986	3089604
ru486 antagonizes the inhibitory action of progesterone on prostacyclin and thromboxane a2 synthesis in cultured rat myometrial explants.	a myometrial explant culture system was developed to investigate the effect of progesterone and the antiprogestagen ru486 on prostacyclin (pgi2) and thromboxane a2 (txa2) synthesis by the rat myometrium. after culture, eicosanoid synthesis was stimulated with arachidonic acid (aa) and the calcium ionophore a23187 (a23187). spontaneous release of eicosanoids was also studied. progesterone inhibited the spontaneous release of pgi2 and txa2 release by myometrial explants in a concentration- and tim ...	1986	3089760
goiter size and thyroid function in an endemic goiter area in northern italy.	thyroid function was studied in a large number of subjects residing in varsi, a town in the province of parma, italy. in this area, endemic goiter associated with moderate iodine deficiency [59 +/- 3 (+/- se) microgram iodine/g creatinine], as defined by who criteria, affects 65% of the population. serum t4, t3, thyroglobulin (tg), and tsh concentrations were measured by ria in 1218 subjects. the tsh response to trh was determined in 108 subjects selected randomly from the groups with different ...	1986	3090091
effects of the binding of myosin light chain kinase on the reactivities of calmodulin lysines.	the effects of the binding of smooth muscle myosin light chain (mlc) kinase on the microenvironments of different regions of calmodulin (cam) were investigated by comparing the acylation rate constants of the seven lysine amino groups of free cam with those of cam complexed with mlc kinase. equimolar amounts of cam and cam-mlc kinase complex were trace labeled with [3h]acetic anhydride in the presence of phenylalanine as a standard nucleophile. after completion of the reaction, equal amounts of ...	1986	3091599
galactosyltransferase purified from rat milk is distinct from the human and bovine enzyme.	n-acetylglucosaminide beta 1, 4-galactosyltransferase (ec 2.4.1.22) was purified from rat milk by affinity chromatography on n-acetylglucosamine-sepharose and alpha-lactalbumin-sepharose columns. the purified enzyme migrated as three polypeptides of relative molecular weight 59,000, 54,000, and 27,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. antiserum raised against the 54k rat protein immunoprecipitated all three polypeptides, suggesting that they share common antigenic sit ...	1986	3091666
mitomycin c skin toxicity studies in mice: reduced ulceration and altered pharmacokinetics with topical dimethyl sulfoxide.	a series of toxicologic and pharmacokinetic studies were performed in balb/c mice administered intradermal (id) mitomycin c (mmc) at doses of .015 to 0.25 mg. dose-dependent skin ulcers were produced at clinically relevant mmc dose levels of .05 and .075 mg (3.6 to 10.7 mg/m2). these doses produced peak ulcers of 0.15 to 0.22 cm2, respectively, one to five days after injection. the integrated ulcer area x time values (area under the curve [auc] ulceration) were 0.89 and 3.11 cm2 x d. a large num ...	1986	3091779
deafferentation elicits increased dopamine-sensitive adenylate cyclase and receptor binding in the olfactory tubercle.	removal of a major non-catecholaminergic output from the olfactory bulb elicits sprouting of dopaminergic axons in the olfactory tubercle. the functional consequences of this increased dopaminergic innervation are presently not known. this study examined the question of whether lesion-induced sprouting of dopaminergic axons is associated with changes in dopamine-sensitive adenylate cyclase and dopamine receptor density in the partially denervated olfactory tubercle. the results indicate that dop ...	1986	3091783
toxigenic vibrio cholerae 01 infections--louisiana and florida.		1986	3092018
laboratory transmission of schistosoma mansoni in brackish waters; survival and infectivity of cercariae.	schistosoma mansoni cercariae were exposed to various dilutions of sea water, both in the absence and in the presence of mice. it was found that whereas 100% and 75% sea water were lethal to the parasites, the parasites survived in lower concentrations of sea water. the lower the concentration the longer was the survival time. similarly, the rate of infection of mice decreased as the concentration of the sea water increased.	1986	3092417
distribution of the adult lactase phenotypes in turkey.	the adult lactase phenotype, lactose absorber or malabsorber, was determined using the lactose tolerance test with breath hydrogen assay in a group of young, healthy, male turks. out of a total of 470 subjects, 135 (28.7%) were lactose absorbers and 335 (71.3%) lactose malabsorbers. the frequency of the 'lactase suppression gene' in this population sample was 0.844 (s.d. 0.012). the frequency of lactase suppression in turkey is intermediate between that in southeast europe and that in agricultur ...	1986	3092418
[heterogeneity of corynebacterium diphtheriae].		1986	3092513
effect of aldose reductase inhibitor (sorbinil) on integration of polyol pathway, pentose phosphate pathway, and glycolytic route in diabetic rat lens.	this study examines the effect of an aldose reductase inhibitor (sorbinil) on the flux of specifically labeled glucose through alternative pathways of metabolism in the lens of normal and diabetic rats 1 wk after the induction of diabetes with alloxan. in the diabetic rat lens, there was an apparent increase in the flux of glucose through the pentose phosphate pathway (ppp), as measured by the difference in the yields of 14co2 from [1-14c]glucose and [6-14c]glucose [c1-c6], this value was 0.087 ...	1986	3093302
the hfr status of pseudomonas aeruginosa is stabilized by integrative suppression.	a temperature-sensitive mutant (dna-11) with the phenotype of a mutant defective in the initiation of dna replication, was isolated from an hfr-like fp2 donor of pseudomonas aeruginosa. reversion of its temperature-sensitive character was achieved by integrative suppression rather than by backmutation or an additional suppressor mutation. the dna-11 mutant proved to be helpful in stabilizing the hfr status of the original host.	1986	3093822
the effect of double filtration plasmapheresis on thyroid hormone economy and thyroid function.	five cases of rheumatoid arthritis (ra), two cases of systemic lupus erythematosus (sle), a case of mixed connective tissue disease (mctd), and a case of cold urticaria were treated with double filtration plasmapheresis (dfp). each aliquot of plasma was obtained at three different points of the dep circuit during the treatment and concentrations of thyroid hormones as well as thyroxine binding globulin (tbg) were measured. despite the removal of considerable amounts of triiodothyronine (t3), thy ...	1986	3095574
[transmission of vibrio cholerae. possible role of the camel].	choleraic vibrio uses the moving means that man discovers to his own use. camels (passive and active vehicles of large quantity of crude water) were experimentally infected with a vibrio (tox. +) and an aeromonas (tox. +). suckling young camels eliminate them for 6 days and grown-up camels for 25 days. this experience, associated with the discovery of vibrio non 01 and shigella in the caravan camel dungs, lead them to become a potential vector of spreading from one oasis to another.	1986	3096512
a stop transfer sequence recognizes receptors for nascent chain translocation across the endoplasmic reticulum membrane.	a stop transfer sequence derived from the extreme carboxyl terminus of membrane igm heavy chain has been shown to confer predictable transmembrane orientation to secretory proteins by aborting translocation of subsequently synthesized protein domains. here we demonstrate that, in certain peptide sequence contexts, the same stop transfer sequence is also capable of initiating domain translocation across the endoplasmic reticulum (er) membrane. translocation directed by a stop transfer sequence is ...	1986	3096576
expression of the escherichia coli lamb gene in vibrio cholerae.	a phage lambda mediated transduction system was devised to facilitate molecular analysis of vibrio cholerae. a lamb expression plasmid, pamh62 was introduced into vibrio cholerae by conjugation. the resulting v. cholerae derivatives harboring pamh62 produced substantial amounts of the lamb protein. this protein was properly inserted into the outer membrane, as suggested by (i) its localization into the cell envelope, (ii) its association with the peptidoglycan layer of the cell wall, and (iii) i ...	1986	2977630
acalculous cholecystitis and cytomegalovirus infection in the acquired immunodeficiency syndrome.		1986	3000250
effect of the hyp mutation and diet-induced hyperparathyroidism on renal parathyroid hormone- and forskolin-stimulated adenosine 3',5'-monophosphate production and brush border membrane phosphate transport.	the present study was undertaken to investigate the effect of the hyp mutation and diet-induced hyperparathyroidism on renal responsiveness to pth and forskolin and to determine whether the renal brush border membrane phosphate transport defect is expressed in the vitamin d- and calcium-deficient hyp mouse. our results indicate that pth is a potent activator of camp synthesis in renal slices obtained from vitamin d-replete normal mice and hyp littermates. however, in the mutants, the amount of c ...	1986	3004890
alpha 2 adrenoceptors modulate the nociceptive jaw-opening reflex in rats.	the jaw-opening reflex in lightly anesthetized rats induced by intrapulpal (left maxillary) electrical tooth pulp stimulation and quantified by the electromyograms (threshold values) recorded from the ipsilateral digastric muscle was used as the experimental pain index. the threshold for the jaw-opening reflex was significantly elevated by clonidine (12.5 to 50 micrograms/kg, i.v.) and was inversely correlated with the frequency of stimulation. the analgesia elicited by clonidine was antagonized ...	1986	3005019
alpha 2 adrenoceptors modulate the nociceptive jaw-opening reflex in rats.	the jaw-opening reflex in lightly anesthetized rats induced by intrapulpal (left maxillary) electrical tooth pulp stimulation and quantified by the electromyograms (threshold values) recorded from the ipsilateral digastric muscle was used as the experimental pain index. the threshold for the jaw-opening reflex was significantly elevated by clonidine (12.5 to 50 micrograms/kg, i.v.) and was inversely correlated with the frequency of stimulation. the analgesia elicited by clonidine was antagonized ...	1986	3005019
cloning of the vibrio cholerae reca gene and construction of a vibrio cholerae reca mutant.	a recombinant plasmid carrying the reca gene of vibrio cholerae was isolated from a v. cholerae genomic library, using complementation in escherichia coli. the plasmid complements a reca mutation in e. coli for both resistance to the dna-damaging agent methyl methanesulfonate and recombinational activity in bacteriophage p1 transductions. after determining the approximate location of the reca gene on the cloned dna fragment, we constructed a defined reca mutation by filling in an xbai site locat ...	1986	3005236
functional reconstitution of the alpha 2-adrenergic receptor with guanine nucleotide regulatory proteins in phospholipid vesicles.	we describe the successful reconstitution of functional interactions between an inhibitory adenylate cyclase-coupled receptor and various nucleotide-binding regulatory proteins in phospholipid vesicles. the receptor is the alpha 2-adrenergic receptor (alpha 2ar) which has been partially purified (approximately 500-5000-fold) from human platelet membranes. the nucleotide-binding regulatory proteins include purified preparations of human erythrocyte ni and ns, bovine retinal transducin and the rec ...	1986	3005307
protective effect of interferon on infections with hand, foot, and mouth disease virus in newborn mice.	the protective effect of interferon on infection with coxsackievirus type a 16 (ca-16) or enterovirus type 71 (ev-71) in newborn mice was examined. subcutaneous administration of murine interferon (muifn-alpha/beta) into the infected mice produced a protective effect against infection with ca-16 or ev-71. it was found that the time of administration of muifn was important in relation to the cycle of infection. protection was observed when muifn was given once daily for several days, from one day ...	1986	3005425
distribution of type d retrovirus sequences in tissues of macaques with simian acquired immune deficiency and retroperitoneal fibromatosis.	horizontally acquired saids retrovirus type 2 (srv-2), a type d retrovirus related to the mason-pfizer monkey virus, has been associated with the simian acquired immunodeficiency syndrome (saids) including retroperitoneal fibromatosis (rf) in several macaque species at two primate research centers. virus specific gene sequences are present in lymphoid and rf tissues but not in muscle tissue of diseased macaques or in any tissues of uninfected normal monkeys. serologic and restriction endonucleas ...	1986	3006332
molecular cloning and expression in escherichia coli k-12 of the gene for a hemagglutinin from vibrio cholerae.	using antiserum to the purified soluble hemagglutinin we isolated an escherichia coli k-12 clone expressing the gene for a hemagglutinin from vibrio cholerae 569b. the plasmid present in this clone was designated ppm471. by deletion analysis with both specific restriction endonucleases and bal 31 nuclease, we localized the gene, to a 0.72-kilobase region of dna, implying a molecular weight of less than 27,000 for the protein. analysis in e. coli k-12 minicells of plasmids containing the cloned g ...	1986	3007362
molecular epidemiology of non-o1 vibrio cholerae and vibrio mimicus in the u.s. gulf coast region.	ten toxigenic vibrio cholerae non-o1 and v. mimicus strains isolated from clinical and environmental sources in the u.s. gulf coast region were examined for genetic relatedness. restriction digest patterns of chromosomal dna and southern blot analysis with a cholera toxin gene probe revealed that the strains exhibited greater genetic divergence than the highly conserved v. cholerae o1 strains isolated from clinical and sewage samples in this region.	1986	3007571
[radiation exposure and risk of radon in the room air of swiss houses].	the radioactive noble gas radon, a member of the natural decay chains of uranium and thorium, enters the indoor environment in regionally highly diverging amounts. subsoil of dwellings, building materials and drinking water are the main sources. in switzerland and in many other countries, exposure of the lung tissue to the short lived radon decay products is the most important component of the radiation dose of the general public. annual doses in areas with crystalline rock of high uranium conte ...	1986	3008463
evidence for the functional role of monosialoganglioside gm1 in synaptic transmission in the rat hippocampus.	the hippocampal slices were incubated with compounds which hydrolyze, modify or bind with sialic acid containing molecules. the efficiency of synaptic transmission was tested in the presence of these compounds. the size of the evoked extracellularly recorded potential following schaffer collateral stimulation was used as an indicator of synaptic transmission efficiency. sodium periodate (10 mm) and sodium perchlorate (59.2 mm) evoked a reversible (after washout) decrease in the size of the popul ...	1986	3008944
cloning and expression of the exfoliative toxin b gene from staphylococcus aureus.	exfoliative toxin type b is produced by bacteriophage group ii strains of staphylococcus aureus and is a causative agent of staphylococcal scalded-skin syndrome. in addition to exfoliative toxin b, most isolates also produce a bacteriocin and are immune to the action of the bacteriocin. these phenotypes, as well as resistance to cadmium, were lost after elimination of a 37.5-kilobase plasmid, prw001, from s. aureus ut0007. transduction and transformation showed that prw001 carries the structural ...	1986	3009410
anticonvulsant activity of piperidinol and (dialkylamino)alkanol esters.	aromatic and heterocyclic esters of 1-methyl-4-piperidinol and 1,4-dimethyl-4-piperidinol and aromatic esters of (dialkylamino)alkanols were prepared and evaluated for antiepileptic activity by the maximal electroshock seizure (mes) and subcutaneous pentylenetetrazole seizure threshold (scmet) assays and for minimal central neurotoxicity by the rotorod ataxia test. the most potent compound, namely the 2-phenylbenzoate (57) of 3-(diethylamino)propanol, was slightly more potent than diphenylhydant ...	1986	3016269
the isolation of genomic recombinants for the human apolipoprotein b gene and the mapping of three common dna polymorphisms of the gene--a useful marker for human chromosome 2.	we have used four independently isolated cdna probes for human apolipoprotein b (apo b), to isolate overlapping genomic recombinants for the 3' portion of the apo b gene. the cdna clones and a unique fragment from the genomic recombinant have been used to identify the human apo b gene in dna from a series of rodent x human somatic cell hybrids. our results provide evidence for the assignment of this gene to the short arm of human chromosome 2 (p23-pter). we have used the cdna probes to identify ...	1986	3017840
cytotoxic and cytotonic factors produced by campylobacter jejuni, campylobacter coli, and campylobacter laridis.	complete toxigenicity studies were performed on 341 strains of campylobacter spp., including 23 nonhuman isolates. toxin profiles based on both cytotonic and cytotoxic factors were determined after analyzing responses in vero, hela, cho and y-1 cells. suckling mouse assays were consistently negative for all culture filtrates tested. toxin-producing strains were frequently encountered among both the human and nonhuman strains of campylobacter jejuni, c. coli, and c. laridis investigated. strains ...	1986	3018039
[requirement of cyclic 3',5'-monophosphate in the production of a permeability increasing factor by vibrio cholerae].		1986	3018312
nuclear thyroid hormone receptors in human cancer tissues.	saturable, high affinity binding sites for 3,5,3' triiodothyronine (t3) were identified in nuclei isolated from human tumors of various origins (breast cancers, other epitheliomas, sarcomas, tumors of the central nervous system). nuclear t3 receptors were present in all samples of primary breast cancer (n = 93; average cmax = 215 fmol/mg dna) and in metastatic tissues originating from breast tumors. a significantly lower t3 binding capacity was found in non-tumor tissues, obtained from breast si ...	1986	3019222
characterization of in vitro dopamine synthesis in the median eminence of rats with haloperidol-induced hyperprolactinemia and bromocriptine-induced hypoprolactinemia.	in order to investigate the mechanism of prl action on dopamine synthesis in tuberoinfundibular dopaminergic (tida) neurons, in vitro dopamine synthesis in the median eminence of hypothalamic slices was compared between hyperprolactinemic and hypoprolactinemic rats, hyper- and hypoprolactinemia were induced in ovariectomized rats by repetitive injections of the dopamine antagonist haloperidol (halo) and the dopamine agonist bromocriptine (bromo), respectively. in vitro dopamine synthesis in tida ...	1986	3019639
the budding mechanism of spikeless vesicular stomatitis virus particles.	virus particles, lacking the spike g-glycoproteins, are produced during infection of vero cells with the vesicular stomatitis virus mutant ts045 at the restrictive temperature 39.5 degrees c. at this temperature the mutated g proteins are blocked in their intracellular transport in the endoplasmic reticulum. we have studied the role of the g proteins in the formation of these spikeless virus particles. the results showed that the spikeless particles contain a full complement of membrane anchors, ...	1986	3019669
the budding mechanism of spikeless vesicular stomatitis virus particles.	virus particles, lacking the spike g-glycoproteins, are produced during infection of vero cells with the vesicular stomatitis virus mutant ts045 at the restrictive temperature 39.5 degrees c. at this temperature the mutated g proteins are blocked in their intracellular transport in the endoplasmic reticulum. we have studied the role of the g proteins in the formation of these spikeless virus particles. the results showed that the spikeless particles contain a full complement of membrane anchors, ...	1986	3019669
effect of tropomyosin on the interactions of actin with actin-binding proteins isolated from pig platelets.	several non-muscle tropomyosins have been reported to lack the ability to polymerize in a head-to-tail manner [dabrowska, r. et al. (1983) j. muscle res. cell motil. 1, 83-92; côté, g.p. (1983) mol. cell. biochem. 57, 127-146]. unlike rabbit skeletal muscle tropomyosin, these proteins could therefore not protect the f-actin microfilaments neither from disassembly or from cross-linking by the other actin-associating factors. however, we have provided evidence that, in vitro, pig platelet tropomyo ...	1986	3019696
effect of tropomyosin on the interactions of actin with actin-binding proteins isolated from pig platelets.	several non-muscle tropomyosins have been reported to lack the ability to polymerize in a head-to-tail manner [dabrowska, r. et al. (1983) j. muscle res. cell motil. 1, 83-92; côté, g.p. (1983) mol. cell. biochem. 57, 127-146]. unlike rabbit skeletal muscle tropomyosin, these proteins could therefore not protect the f-actin microfilaments neither from disassembly or from cross-linking by the other actin-associating factors. however, we have provided evidence that, in vitro, pig platelet tropomyo ...	1986	3019696
modulation of receptor mediated leukotriene release in the perfused heart.	the chemotactic peptide n-formyl-methionyl-leucyl-phenylalanine (fmlp) induces peptide leukotriene release at concentrations of 20-25 pmol/ml 3 min after the start of fmlp infusion. fmlp-induced leukotriene release in rabbit hearts is not blocked by the leukotriene receptor antagonist fpl-55712 at concentrations that totally antagonize the hemodynamic effects of exogenously infused peptide leukotrienes. moreover, propyl gallate, a lipoxygenase inhibitor, does not block fmlp-induced leukotriene r ...	1986	3019823
modulation of receptor mediated leukotriene release in the perfused heart.	the chemotactic peptide n-formyl-methionyl-leucyl-phenylalanine (fmlp) induces peptide leukotriene release at concentrations of 20-25 pmol/ml 3 min after the start of fmlp infusion. fmlp-induced leukotriene release in rabbit hearts is not blocked by the leukotriene receptor antagonist fpl-55712 at concentrations that totally antagonize the hemodynamic effects of exogenously infused peptide leukotrienes. moreover, propyl gallate, a lipoxygenase inhibitor, does not block fmlp-induced leukotriene r ...	1986	3019823
enhanced relaxation response of canine coronary artery to isoproterenol and salbutamol after removal of endothelial cells.	the importance of the endothelium for isoproterenol- and salbutamol-induced relaxation of canine coronary artery was examined. the relaxation effect of isoproterenol and salbutamol was significantly enhanced (p less than 0.05) upon removal of the coronary artery endothelium. in contrast, the relaxation effect of acetylcholine was completely abolished. these results indicate that canine coronary endothelium modulates beta-adrenoceptor-mediated relaxation, and that the role of the endothelium in a ...	1986	3019826
enhanced relaxation response of canine coronary artery to isoproterenol and salbutamol after removal of endothelial cells.	the importance of the endothelium for isoproterenol- and salbutamol-induced relaxation of canine coronary artery was examined. the relaxation effect of isoproterenol and salbutamol was significantly enhanced (p less than 0.05) upon removal of the coronary artery endothelium. in contrast, the relaxation effect of acetylcholine was completely abolished. these results indicate that canine coronary endothelium modulates beta-adrenoceptor-mediated relaxation, and that the role of the endothelium in a ...	1986	3019826
up-regulation of brain [3h]diazepam binding sites in chronic caffeine treated rats.	brain [3h]diazepam and [3h]l-phenylisopropyladenosine binding sites in caffeine treated (75 mg/kg/day, i.p. 12 days) and caffeine withdrawn (30 days) rats were examined. treatment with caffeine (75 mg/kg/day) for 12 days increases the bmax (maximum binding capacity) for [3h]diazepam binding by 30.9% whereas the same treatment increases the bmax for [3h]l-pia binding by 165%. the bmax for [3h]diazepam binding sites returns to slightly below control levels but [3h]l-pia binding sites remain elevat ...	1986	3019827
up-regulation of brain [3h]diazepam binding sites in chronic caffeine treated rats.	brain [3h]diazepam and [3h]l-phenylisopropyladenosine binding sites in caffeine treated (75 mg/kg/day, i.p. 12 days) and caffeine withdrawn (30 days) rats were examined. treatment with caffeine (75 mg/kg/day) for 12 days increases the bmax (maximum binding capacity) for [3h]diazepam binding by 30.9% whereas the same treatment increases the bmax for [3h]l-pia binding by 165%. the bmax for [3h]diazepam binding sites returns to slightly below control levels but [3h]l-pia binding sites remain elevat ...	1986	3019827
inhibition and reversal of endotoxin-, aggregated igg- and paf-induced hypotension in the rat by sri 63-072, a paf receptor antagonist.	platelet activating factor (paf) given intravenously produces systemic hypotension in the rat. similar effects can be induced using endotoxin or heat-aggregated igg challenges, which are thought to involve endogenous paf release. extending this concept, we have examined the ability of the paf antagonist sri 63-072 to inhibit or reverse systemic hypotension induced with paf, heat-aggregated igg or endotoxin 0111-b4 in rats. at 100 ng kg-1 paf, there occurred a 38.6 +/- 5.1% decrease in carotid me ...	1986	3019921
inhibition and reversal of endotoxin-, aggregated igg- and paf-induced hypotension in the rat by sri 63-072, a paf receptor antagonist.	platelet activating factor (paf) given intravenously produces systemic hypotension in the rat. similar effects can be induced using endotoxin or heat-aggregated igg challenges, which are thought to involve endogenous paf release. extending this concept, we have examined the ability of the paf antagonist sri 63-072 to inhibit or reverse systemic hypotension induced with paf, heat-aggregated igg or endotoxin 0111-b4 in rats. at 100 ng kg-1 paf, there occurred a 38.6 +/- 5.1% decrease in carotid me ...	1986	3019921
phase i/ii trials of wr-2721 and cis-platinum.	renal dysfunction is a well-known dose-limiting toxicity of cis-platinum. previous studies demonstrated a 32% incidence of nephrotoxicity following a 100 mg/m2 single dose of cis-platinum with mannitol diuresis. wr-2721 is an aminothiol which in the animal model improves renal tolerance to cis-platinum by factors of 1.3 to 1.7. phase i trials were initiated to establish the toxicity when wr-2721 was given prior to escalating doses of cis-platinum. fifty-two patients received 161 courses of wr-27 ...	1986	3019969
phase i/ii trials of wr-2721 and cis-platinum.	renal dysfunction is a well-known dose-limiting toxicity of cis-platinum. previous studies demonstrated a 32% incidence of nephrotoxicity following a 100 mg/m2 single dose of cis-platinum with mannitol diuresis. wr-2721 is an aminothiol which in the animal model improves renal tolerance to cis-platinum by factors of 1.3 to 1.7. phase i trials were initiated to establish the toxicity when wr-2721 was given prior to escalating doses of cis-platinum. fifty-two patients received 161 courses of wr-27 ...	1986	3019969
hepatocellular adenoma and nodular regenerative hyperplasia of the liver in a young man.	a 26-year-old man had a massive intraabdominal hemorrhage from a hepatocellular adenoma (hca). the tumor arose within a liver that demonstrated generalized nodular regenerative hyperplasia. the patient had no factors predisposing to either hca or nodular regenerative hyperplasia (nrh) of the liver. although rare, hca should be included in the differential diagnosis of spontaneous intraperitoneal hemorrhage even in young men. the coexistence of hca and nrh of the liver in this patient may indicat ...	1986	3020120
hepatocellular adenoma and nodular regenerative hyperplasia of the liver in a young man.	a 26-year-old man had a massive intraabdominal hemorrhage from a hepatocellular adenoma (hca). the tumor arose within a liver that demonstrated generalized nodular regenerative hyperplasia. the patient had no factors predisposing to either hca or nodular regenerative hyperplasia (nrh) of the liver. although rare, hca should be included in the differential diagnosis of spontaneous intraperitoneal hemorrhage even in young men. the coexistence of hca and nrh of the liver in this patient may indicat ...	1986	3020120
carbachol- and norepinephrine-stimulated phosphoinositide metabolism in rat brain: effect of chronic cholinesterase inhibition.	activation of cholinergic muscarinic receptors leads to several biochemical events including an increased turnover of phosphoinositides. in this study we have investigated whether repeated administration of the organophosphorus insecticide disulfoton, known to cause the development of tolerance to this compound, would affect phosphoinositide metabolism in rat brain. basal and carbachol-stimulated phosphoinositide metabolism were measured in cerebral cortex slices, by measuring the accumulation o ...	1986	3020234
carbachol- and norepinephrine-stimulated phosphoinositide metabolism in rat brain: effect of chronic cholinesterase inhibition.	activation of cholinergic muscarinic receptors leads to several biochemical events including an increased turnover of phosphoinositides. in this study we have investigated whether repeated administration of the organophosphorus insecticide disulfoton, known to cause the development of tolerance to this compound, would affect phosphoinositide metabolism in rat brain. basal and carbachol-stimulated phosphoinositide metabolism were measured in cerebral cortex slices, by measuring the accumulation o ...	1986	3020234
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.	two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...	1986	3020551
evidence that a human soluble beta-galactoside-binding lectin is encoded by a family of genes.	two cdna clones were isolated by immunoscreening a human hepatoma cdna library with an antiserum that bound specifically to a human soluble beta-galactoside-binding lectin with mr of approximately 14,000. the deduced amino acid sequences of the inserts of these two clones show considerable homology with each other, the sequence of chicken skin beta-galactoside-binding lectin, and eight peptides derived from purified human lung lectin of mr approximately 14,000. however, the sequence differences ...	1986	3020551
exposure of sheep to natural aerosols of foot-and-mouth disease virus.	sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ...	1986	3020658
[differential diagnosis of viral diarrheas in calves].	there is a description of the enzymoimmunologic method (elisa), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. it is shown by a comparison with the results obtained by electron microscopy that the elisa method provides by up to 50% higher capture rate and the reaction is highly specific. the method can also be applied to a detection of human rotavirus in the children's stool.	1986	3020762
[differential diagnosis of viral diarrheas in calves].	there is a description of the enzymoimmunologic method (elisa), which was used for demonstration of rotaviruses and coronaviruses in the samples of excrements of the calves suffering from diarrheas. it is shown by a comparison with the results obtained by electron microscopy that the elisa method provides by up to 50% higher capture rate and the reaction is highly specific. the method can also be applied to a detection of human rotavirus in the children's stool.	1986	3020762
subtypes and functions of alpha-adrenoceptors.		1986	3020944
dna distributions in human normal, precancerous and cancerous breast tissue. i. ploidy and cell cycle distribution.	dna distributions were recorded flow cytometrically in 19 normal, 23 precancerous tissues with epithelial proliferation and cell atypia (p1), 7 carcinomata lobularia in situ and intraductale carcinomas (p2) and in 44 invasive carcinomas of the human breast. the results were compared to histological findings, tumor staging and grading, lymph node involvement and age of the patients. dna diploidy was found in all normal, 21 p1 tissues, 6 p2 tissues and 12 carcinomas; dna aneuploidy in 2 p1 tissues ...	1986	3021084
dna distributions in human normal, precancerous and cancerous breast tissue. i. ploidy and cell cycle distribution.	dna distributions were recorded flow cytometrically in 19 normal, 23 precancerous tissues with epithelial proliferation and cell atypia (p1), 7 carcinomata lobularia in situ and intraductale carcinomas (p2) and in 44 invasive carcinomas of the human breast. the results were compared to histological findings, tumor staging and grading, lymph node involvement and age of the patients. dna diploidy was found in all normal, 21 p1 tissues, 6 p2 tissues and 12 carcinomas; dna aneuploidy in 2 p1 tissues ...	1986	3021084
structure and activities of a variant ubiquitin sequence from bakers' yeast.	ubiquitin is an extremely conserved protein, with an identical sequence throughout the animal kingdom. however, the gene sequence of the yeast protein [ozkaynak, e., finley, d., & varshavsky, a. (1984) nature (london) 312, 663-666] predicts three amino acid differences. this implies that some functions or binding interactions of ubiquitin are different in yeast and animal cells. in an effort to define these differences, ubiquitin has been purified to homogeneity from bakers' yeast and characteri ...	1986	3021209
spermine antagonises the effects of dexamethasone, glucagon and cyclic amp in increasing the activity of phosphatidate phosphohydrolase in isolated rat hepatocytes.	rat hepatocytes were incubated in monolayer culture, under serum free conditions, for 8 h. glucagon (10 nm), 8-(4-chlorophenylthio)adenosine 3',5'-cyclic monophosphate (100 microm) and dexamethasone (100 nm) increased the activity of phosphatidate phosphohydrolase by approx. 2-, 3.6- and 3.3-fold, respectively. spermine alone had no significant effect. spermine (2.5 mm) almost completely inhibited the glucagon induced increase in phosphohydrolase activity. it only partially inhibited the dexamet ...	1986	3021529
hemolysin production and cloning of two hemolysin determinants from classical vibrio cholerae.	the hemolytic activity of 20 classical and 3 el tor strains of v. cholerae o1 was examined phenotypically and genetically. the el tor strains lysed bovine, chicken, human, rabbit, and sheep erythrocytes (rbcs), while the classical strains lysed only chicken and rabbit rbcs. the assay was done with rbcs in tris-nacl buffer, since phosphate-buffered saline was found to inhibit hemolytic activity. hemolytic activity in culture supernatants from el tor strains was more sensitive to heat inactivation ...	1986	3021628
thymidine kinase with altered substrate specificity of acyclovir resistant varicella-zoster virus.	the acyclovir resistant mutant of varicella-zoster virus acv-r (a 8) induced the same level of thymidine kinase activity in infected cells as the parent kawaguchi strain. however, it induced less deoxycytidine kinase activity and did not induce phosphorylating activity for the nucleotide analogue, 9-(2 hydroxy-ethoxymethyl)-guanine-(acyclovir). another acyclovir resistant mutant, acv-r (a 4), which is cross-resistant to phosphonoacetate and is thought to be a viral dna polymerase mutant, induced ...	1986	3022711
identification and characterization of mg2+-dependent phosphotyrosyl protein phosphatase from rat liver cytosol.	although highly purified preparations of mg2+-dependent phosphoseryl protein phosphatase (also designated phosphatase ia or phosphatase 2c) dephosphorylated phosphotyrosyl histone, the activity has been resolved from phosphatase ia by polyacrylamide gel electrophoresis at ph 9.5. this novel phosphotyrosyl-specific protein phosphatase absolutely requires mg2+ or mn2+ for activity, is inhibited by zn2+, vanadate and fluoride, and has an optimal ph of 9.0 and mr = 50,000. certain properties of this ...	1986	3022716
immortalization of early embryonic cell derivatives after the transfer of the early region of simian virus 40 into f9 teratocarcinoma cells.	f9 embryonal carcinoma (ec) cells were transformed using a recombinant plasmid carrying simian virus 40 (sv40) early genes linked to the promoter-enhancer region of the early transcription unit 1a (e1a) of adenovirus type 5. one clone of transformed cells, f9-k4b2, contained several integrated copies of the plasmid and expressed sv40 early genes (t antigens) only when it was induced to differentiate in vitro by the addition of retinoic acid and dibutyryl-camp. from the cell population positive f ...	1986	3023162
cross-linking of a growth hormone releasing factor-binding protein in anterior pituitary cells.	growth hormone-releasing factor (grf) stimulates the release of growth hormone from the anterior pituitary and is related to the peptides of the glucagon/secretin family. although the mechanism of action of this hormone has been studied in considerable detail, little is known concerning the grf receptor itself. we have attempted to label the grf receptor by chemically coupling the 125i-grf analog [his1, nle27]-hgrf(1-32)-nh2 (grfa) (where nle is norleucine) to plated rat anterior pituitary cells ...	1986	3023363
cross-linking of a growth hormone releasing factor-binding protein in anterior pituitary cells.	growth hormone-releasing factor (grf) stimulates the release of growth hormone from the anterior pituitary and is related to the peptides of the glucagon/secretin family. although the mechanism of action of this hormone has been studied in considerable detail, little is known concerning the grf receptor itself. we have attempted to label the grf receptor by chemically coupling the 125i-grf analog [his1, nle27]-hgrf(1-32)-nh2 (grfa) (where nle is norleucine) to plated rat anterior pituitary cells ...	1986	3023363
fk 33-824, a met-enkephalin analog, blocks corticotropin-releasing hormone-induced adrenocorticotropin secretion in normal subjects but not in patients with cushing's disease.	to further elucidate the site of action of opioids on the pituitary-adrenal axis, we studied the effect of d-ala2,mephe4,met-(o)enkephalin-ol (sandoz, fk 33-824) on plasma acth and beta-endorphin immunoreactivity and serum cortisol in 7 normal subjects and 11 patients with cushing's syndrome (cushing's disease, n = 7; adrenal adenoma, n = 2; ectopic cushing's syndrome, n = 2) after administration of human corticotropin-releasing hormone (hcrh). hcrh (0.1 mg; bachem) was injected iv after pretrea ...	1986	3023423
rash mutants deficient in gtp binding.	single amino acid substitutions were introduced into a region of the rash protein (residues 116, 117, and 119) homologous to a variety of diverse gtp-binding proteins. each of the mutant p21 proteins displayed a significant reduction (10- to 5,000-fold) in gtp binding affinity. activated rash proteins deficient in gtp binding were unaltered in their ability to morphologically transform nih 3t3 cells.	1986	3097518
[characteristics of the functional antigens of neisseria meningitidis group b].	the work deals with the characterization of the functional antigens of n. meningitidis, group b. analysis of the total extract of n. meningitidis strain b16b6 with the use of sera obtained from patients with meningococcal infection, nasopharyngitis, salmonellosis, as well as from meningococcus carriers and healthy donors, by immune blotting techniques and the affinity isolation of the functional antigens indicate that protein antigen with a molecular weight of 13-15 kd is the main common antigen ...	1986	3098003
myocardial oxygen supply during hypocapnia and hypercapnia in the dog.	it has been postulated that a coronary vasoconstriction during hypocapnia might be opposed by a compensating coronary vasodilatation due to impaired myocardial oxygen supply. the present study was performed first to examine whether a maximal decline in coronary sinus (cs) oxygen content was reached during hypocapnia. during hypercapnia a myocardial "over perfusion" has been demonstrated. the second purpose of the present study was to examine whether a myocardial "over perfusion" is essential to ...	1986	3098394
a strain of multiform cryptococcus neoformans.		1986	3100152
etiological and clinical characteristics of diarrhea in young adults, with special reference to shigella gastroenteritis.		1986	3100995
[characterization of pseudomonas aeruginosa isolates from intensive care units by sero-, lyso- and pyocin typing].		1986	3101296
nucleotide sequence of ompv, the gene for a major vibrio cholerae outer membrane protein.	the nucleotide sequence of the ompv gene of vibrio cholerae was determined. the product of the gene is a 28,000 dalton protein which, after the removal of a 19 amino acid signal sequence, produces a mature outer membrane protein of 26,000 daltons. the cleavage site was determined by amino-terminal amino acid sequencing of the purified mature protein. the dna upstream of the gene shows the presence of a typical promoter region as judged from the escherichia coli consensus information; however, th ...	1986	3031428
carbamate kinase of lactobacillus buchneri ncdo110. i. purification and properties.	carbamate kinase has been prepared from lactobacillus buchneri ncdo110. an approximately 91-fold increase in the specific activity of the enzyme was achieved. the purified extract exhibited a single band following polyacrylamide gel electrophoresis. the apparent molecular weight as determined by gel electrophoresis was about 97,000. the enzyme is stable for 2 weeks at -20 degrees c. maximum enzymatic activity was observed at 30 degrees c and ph 5.4 in 0.1 m acetate buffer. l. buchneri carbamate ...	1986	3030360
carbamate kinase of lactobacillus buchneri ncdo110. i. purification and properties.	carbamate kinase has been prepared from lactobacillus buchneri ncdo110. an approximately 91-fold increase in the specific activity of the enzyme was achieved. the purified extract exhibited a single band following polyacrylamide gel electrophoresis. the apparent molecular weight as determined by gel electrophoresis was about 97,000. the enzyme is stable for 2 weeks at -20 degrees c. maximum enzymatic activity was observed at 30 degrees c and ph 5.4 in 0.1 m acetate buffer. l. buchneri carbamate ...	1986	3030360
interaction of kappa receptor agonists with ca2+ channel antagonists in the modulation of hypothermia.	administration of the opiate u-50,488h (3-20 mg/kg s.c.), a selective kappa receptor agonist, produced a dose-dependent decrease of rectal temperature in rats. this hypothermic effect of u-50,488h was accompanied by an enhanced activity of ca2+/mg2+ atpase in crude synaptosomal (p2) fractions obtained from hypothalamus but not from cortex or cerebellum. mg2+ atpase activity in these regions was not altered by u-50,488h (15 mg/kg s.c.). naloxone (5 mg/kg) partially and mr2266 (5 mg/kg) completely ...	1986	3028838
interaction of kappa receptor agonists with ca2+ channel antagonists in the modulation of hypothermia.	administration of the opiate u-50,488h (3-20 mg/kg s.c.), a selective kappa receptor agonist, produced a dose-dependent decrease of rectal temperature in rats. this hypothermic effect of u-50,488h was accompanied by an enhanced activity of ca2+/mg2+ atpase in crude synaptosomal (p2) fractions obtained from hypothalamus but not from cortex or cerebellum. mg2+ atpase activity in these regions was not altered by u-50,488h (15 mg/kg s.c.). naloxone (5 mg/kg) partially and mr2266 (5 mg/kg) completely ...	1986	3028838
tifluadom, a kappa-opiate agonist, acts as a peripheral cholecystokinin receptor antagonist.	tifluadom, a benzodiazepine kappa-opiate agonist, stereoselectively inhibited the binding of 125i-cck to pancreatic membranes (ic50 = 47 nm). several other opiate agonists were ineffective. scatchard analysis indicated the inhibition of cck binding by tifluadom was competitive in nature. tifluadom (1 microm) did not displace 125i-cck binding to brain tissue or 125i-gastrin binding to fundic glands. in the isolated guinea pig gallbladder, tifluadom antagonized cck-8 induced contractions with an e ...	1986	3027627
familial human parvovirus infection associated with anemia in siblings with heterozygous beta-thalassemia.		1986	3009639