Publications

the hypoxic ventilatory response of rats with increased blood oxygen affinity.	experimental work has established an interspecific relationship between the threshold for the hypoxic ventilatory response and the 'knee' of the hb-o2 dissociation curve. however, whether this relationship exists intraspecifically remains unclear. to examine the problem further rats were treated with sodium cyanate (naocn) to lower p50 and their hypoxic ventilatory response was measured. naocn treated rats had a lower pao2 and higher hct than control rats. there was no difference between the con ...	1986	3809758
the hypoxic ventilatory response of rats with increased blood oxygen affinity.	experimental work has established an interspecific relationship between the threshold for the hypoxic ventilatory response and the 'knee' of the hb-o2 dissociation curve. however, whether this relationship exists intraspecifically remains unclear. to examine the problem further rats were treated with sodium cyanate (naocn) to lower p50 and their hypoxic ventilatory response was measured. naocn treated rats had a lower pao2 and higher hct than control rats. there was no difference between the con ...	1986	3809758
the addition of nitrous oxide to halothane decreases renal and splanchnic flow and increases cerebral blood flow in rats.	thirteen male sprague-dawley rats were anaesthetized with halothane and catheters were placed in the femoral artery and left ventricle. the animals breathed spontaneously through a tracheostomy throughout the investigation. after the surgical preparation, the inspired halothane concentration was maintained at 1.5 vol% (f/o2 = 0.3). after a 30-min stabilization period, strontium-85 radiolabelled microspheres (15 +/- 1 micron) were injected to the left ventricle for determination of cardiac output ...	1986	3942673
the addition of nitrous oxide to halothane decreases renal and splanchnic flow and increases cerebral blood flow in rats.	thirteen male sprague-dawley rats were anaesthetized with halothane and catheters were placed in the femoral artery and left ventricle. the animals breathed spontaneously through a tracheostomy throughout the investigation. after the surgical preparation, the inspired halothane concentration was maintained at 1.5 vol% (f/o2 = 0.3). after a 30-min stabilization period, strontium-85 radiolabelled microspheres (15 +/- 1 micron) were injected to the left ventricle for determination of cardiac output ...	1986	3942673
iodipamide uptake by rat liver plasma membrane vesicles enriched in the sinusoidal fraction: evidence for a carrier-mediated transport dependent on membrane potential.	iodipamide, a cholecystographic agent, is known to be taken up by isolated hepatocytes by a mechanism similar or identical with the inward transport of bile salts (petzinger, e., joppen, c. and frimmer, m. (1983) naunyn-schmiedeberg's arch. pharmacol. 322, 174-179). to elucidate its mode of transport, uptake of iodipamide was studied by rapid-filtration techniques on plasma membrane vesicles enriched in the sinusoidal fraction. uptake was found to be dependent upon the temperature, the intravesi ...	1986	3942739
fate of n-(2-hydroxypropyl)methacrylamide copolymers with pendent galactosamine residues after intravenous administration to rats.	n-(2-hydroxypropyl)methacrylamide (hpma) copolymers bearing galactosamine residues accumulate in the liver after intravenous administration to rats (duncan, r., kopecek, j., rejmanová, p. and lloyd, j.b. (1983) biochim. biophys. acta 755, 518-521). in this study hpma copolymers bearing pendent galactosamine residues (1.0-11.6 mol%) were injected intravenously into rats and their rates of blood clearance and liver accumulation were measured. a level of substitution of 4 mol% was found to be suffi ...	1986	3942780
characterization of estrogen and progesterone receptors and the dissociated regulation of growth and progesterone receptor stimulation by estrogen in mda-mb-134 human breast cancer cells.	we have examined the properties of the estrogen receptor and progesterone receptor in mda-mb-134 human breast cells and have evaluated the effects of estrogen on cell proliferation and progesterone receptor levels in these cells as indices of hormonal sensitivity. these cells contain high levels of estrogen receptor (approximately 1.5 pmol/mg dna) and low levels of progesterone receptor (0.15 pmol/mg dna). more than 80% of the estrogen receptor is found in the nuclear fraction in the absence of ...	1986	3943090
characterization of estrogen and progesterone receptors and the dissociated regulation of growth and progesterone receptor stimulation by estrogen in mda-mb-134 human breast cancer cells.	we have examined the properties of the estrogen receptor and progesterone receptor in mda-mb-134 human breast cells and have evaluated the effects of estrogen on cell proliferation and progesterone receptor levels in these cells as indices of hormonal sensitivity. these cells contain high levels of estrogen receptor (approximately 1.5 pmol/mg dna) and low levels of progesterone receptor (0.15 pmol/mg dna). more than 80% of the estrogen receptor is found in the nuclear fraction in the absence of ...	1986	3943090
a functional organization of on and off pathways in the rabbit retina.	intracellular electrophysiological recordings were obtained from amacrine and ganglion cells in an isolated, superfused retina-eyecup preparation of the rabbit. cells were characterized physiologically, after which cell-staining was accomplished by intracellular iontophoresis of hrp. a computer-assisted image-processing system was used to study the dendritic stratification pattern of hrp-labeled neurons within the inner plexiform layer (ipl). our results support the concept that the ipl is funct ...	1986	3944611
modification of excitation and inhibition evoked in dentate gyrus by perforant path stimulation: effects of aminophylline and kindling.	rats were implanted with chronic electrodes to stimulate the perforant path and record the elicited monosynaptic evoked potentials from the dentate gyrus of the hippocampal formation. dentate responses were examined in awake and anesthetized animals after exposure to saline and aminophylline (100 mg/kg, ip). in the awake animal, aminophylline treatment did not significantly alter the threshold or elicited amplitude of either the excitatory post-synaptic potential (epsp) or the population spike ( ...	1986	3945669
modification of excitation and inhibition evoked in dentate gyrus by perforant path stimulation: effects of aminophylline and kindling.	rats were implanted with chronic electrodes to stimulate the perforant path and record the elicited monosynaptic evoked potentials from the dentate gyrus of the hippocampal formation. dentate responses were examined in awake and anesthetized animals after exposure to saline and aminophylline (100 mg/kg, ip). in the awake animal, aminophylline treatment did not significantly alter the threshold or elicited amplitude of either the excitatory post-synaptic potential (epsp) or the population spike ( ...	1986	3945669
impact of epidemic cholera in a previously uninfected island population: evaluation of a new seroepidemiologic method.	during an investigation of a 1982 cholera outbreak in truk, an area without endemic cholera, 254 post-outbreak serum specimens were collected from ill and well inhabitants of a single island. these were compared with 57 specimens collected in truk in 1964, when heat-labile toxin-producing enterotoxigenic escherichia coli was presumably endemic but cholera did not exist. the serum was tested for vibriocidal antibody and antitoxic antibodies to cholera toxin and heat-labile toxin and the ratio of ...	1986	3946388
comparison of radiosensitivities of human autologous normal and neoplastic thyroid epithelial cells.	studies were conducted to examine differences between the radiosensitivities of normal and neoplastic epithelial cells of the human thyroid. freshly excised thyroid tissues from the tumours of eight patients with papillary carcinoma (pc) and five with follicular adenoma (fa) were cultured in vitro separately from normal thyroid tissue obtained from the surgical margins of the same patients. plating efficiency of unirradiated control tissue was lower, on average for tumour tissue compared with no ...	1986	3947820
neurotensin excites neurons in the arcuate nucleus of the rat hypothalamus in vitro.	the effects of neurotensin (nt) on 41 spontaneously active neurons in the rat hypothalamic arcuate nucleus (arc) were determined using an in vitro brain slice technique. addition of nt (1 microm to 10 nm) to the perifusing medium allowed the action of known concentrations of nt to be examined. concentrations of peptide greater than 10 nm evoked excessive depolarization of sensitive neurons resulting in long periods of postexcitatory inhibition. at 10 nm, nt evoked reversible and repeatable excit ...	1986	3947977
sequential monitoring of peripheral blood lymphocyte subsets in rats.	the methodology of enumeration of t cell subsets in humans is well established and has widespread clinical application. sequential monitoring of lymphocyte subsets in small animals requires a consistent and reliable methodology for staining and enumerating lymphocyte populations. the optimal conditions for enumerating subclasses of rat peripheral blood lymphocytes with the spectrum iii flow cytometer are described. the sources of biological variation and technical error that were considered in o ...	1986	3948606
growth hormone modulates serum cholinesterase.	adult male rats have lower serum cholinesterase activity levels than adult female rats and hypophysectomized male rats have higher activity levels than sham-operated males (similar to control females). gh administered to hypophysectomized male rats abolishes the effect of hypophysectomy on serum cholinesterase. adult male rats treated neonatally with monosodium l-glutamate to induce arcuate nucleus lesions of the hypothalamus have higher serum cholinesterase activities and decreased serum gh con ...	1986	3948777
effect of a reca mutation on cholera toxin gene amplification and deletion events.	the cholera toxin operon (ctxab) is located on a 7-kilobase pair variable genetic element which undergoes genetic duplication and amplification events in vibrio cholerae. amplification of the ctx genetic element was investigated by substituting the resident ctx loci of two v. cholerae strains with a dna fragment encoding resistance to kanamycin. although these strains were not normally resistant to greater than 150 micrograms of kanamycin per ml, spontaneous derivatives could be obtained that gr ...	1986	3949714
the impact of physico-chemical stress on the toxigenicity of vibrio cholerae.	the expression of toxigenicity by vibrio cholerae, before and after exposure to various conditions of salinity, ph and cation composition and concentration, has been measured. exposure to these conditions did not select for hyper- or hypo-toxigenic strains. this suggests that toxigenic v. cholerae o1 are unlikely to lose their toxigenicity when exposed to environmental stress and that v. cholerae toxin production is not a response to the stresses included in this study. these results are consist ...	1986	3950396
clonal spread of multiply resistant strains of vibrio cholerae o1 in somalia.		1986	3950453
infection by choleraphage phi 138: bacteriophage dna and replicative intermediates.	choleraphage phi 138 contains a linear, double-stranded, circularly permuted dna molecule of 30 x 10(6) daltons or 45 kilobase pairs. upon infection, the host dna is degraded, and synthesis of phage-specific dna is detectable 20 min after infection. the phage utilizes primarily the host dna degradation products for its own dna synthesis. a physical map of phi 138 dna was constructed with the restriction endonucleases bg/ii, hindiii, and psti. a concatemeric replicative dna intermediate equivalen ...	1986	3951021
effects of dietary fat on mammary development relative to age and hormones in balb/c mice.	earlier studies reported that mammary ducts grew faster if the 10% fat in the diet was composed of oils containing polyunsaturated fatty acids (corn oil: co) compared to hydrogenated cottonseed oil (hcto), which is devoid of such fatty acids. these experiments were primarily carried out in immature mice and left unanswered questions regarding the effects of dietary fats on more differentiated stages of mammary development. the use of transplanted ducts permitted the study of mammary growth rates ...	1986	3952083
interactions of vasoactive intestinal polypeptide and cholecystokinin octapeptide on the control of gallbladder contraction.	the gallbladder is supplied by three types of vagal fibers: cholinergic, cholecystokinin (cck)-ergic, and vasoactive intestinal polypeptide (vip)-ergic. most previous studies on the interaction of vip and cck on gallbladder contraction have been in vitro. in this study we evaluate this interaction more fully in vivo using six dogs with chronic bile fistula. dose-response curves were constructed to cck-8 alone and to vip alone. the effect of graded doses of vip on maximal response to cck-8 was al ...	1986	3952671
interactions of vasoactive intestinal polypeptide and cholecystokinin octapeptide on the control of gallbladder contraction.	the gallbladder is supplied by three types of vagal fibers: cholinergic, cholecystokinin (cck)-ergic, and vasoactive intestinal polypeptide (vip)-ergic. most previous studies on the interaction of vip and cck on gallbladder contraction have been in vitro. in this study we evaluate this interaction more fully in vivo using six dogs with chronic bile fistula. dose-response curves were constructed to cck-8 alone and to vip alone. the effect of graded doses of vip on maximal response to cck-8 was al ...	1986	3952671
lack of evidence of hepatitis d (delta) infection in newfoundland and labrador.	epidemiologic knowledge of hepatitis d virus (hdv) infection is limited. a seroepidemiologic study was undertaken to determine the prevalence of the infection in newfoundland and labrador. between october 1983 and october 1985 over 200 people were recognized through routine serodiagnosis and screening as having hepatitis b seromarkers. a total of 223 serum samples from 186 of these people were tested for anti-hdv. the subjects were mainly asymptomatic carriers of hepatitis b surface antigen or p ...	1986	3955485
lack of evidence of hepatitis d (delta) infection in newfoundland and labrador.	epidemiologic knowledge of hepatitis d virus (hdv) infection is limited. a seroepidemiologic study was undertaken to determine the prevalence of the infection in newfoundland and labrador. between october 1983 and october 1985 over 200 people were recognized through routine serodiagnosis and screening as having hepatitis b seromarkers. a total of 223 serum samples from 186 of these people were tested for anti-hdv. the subjects were mainly asymptomatic carriers of hepatitis b surface antigen or p ...	1986	3955485
isolation and characterization of two sialyloligosaccharides containing n-acetyllactosamine from pregnancy urine.		1986	3955566
nadph/nadh-dependent cold-labile glutamate dehydrogenase in azospirillum brasilense. purification and properties.	a cold-labile glutamate dehydrogenase (gdh, ec 1.4.1.3) has been purified to homogeneity from the crude extracts of azospirillum brasilense. the purified enzyme shows a dual coenzyme specificity, and both the nadph and nadh-dependent activities are equally cold-sensitive. the enzyme is highly specific for the substrates 2-oxoglutarate and glutamate. kinetic studies with gdh indicate that the enzyme is primarily designed to catalyse the reductive amination of 2-oxoglutarate. the nadp+-linked acti ...	1986	3956501
proximal and distal changes in collagen content of peripheral nerve that follow transection and crush lesions.	collagen content of rat sciatic nerve was measured 10 weeks after either nerve transection or nerve crush. nerve transection led to a significant increase in fascicular collagen in nerve segments 2.5 mm proximal and distal to the injury site. remote from the transection, fascicular collagen was also significantly increased, this effect being most marked distally. nerve crush by comparison resulted in only a small increase in fascicular collagen, significantly less than after transection. the gre ...	1986	3956663
an analysis of class i antigens of man and other species by one-dimensional ief and immunoblotting.	a procedure for the molecular identification of mhc class i products based on 1-d ief and subsequent immunoblotting is described. optimal conditions for 1-d ief, the electrophoretic transfer of proteins out of denaturing, nonionic detergent-containing gels to nitrocellulose, and the requisite antibodies, both polyclonal and monoclonal, for the visualization of class i heavy chains have been established. cross-reactivity of antibodies has enabled the biochemical analysis of class i heavy chains i ...	1986	3957414
purification and partial characterization of a non-o1 vibrio cholerae hemolysin that cross-reacts with thermostable direct hemolysin of vibrio parahaemolyticus.	a newly identified hemolysin (nag-rtdh), which is related to the thermostable direct hemolysin (vp-tdh) of vibrio parahaemolyticus produced by non-o1 vibrio cholerae, was studied. nag-rtdh was purified by successive column chromatographies on deae-cellulose and an immunoaffinity column coupled with anti-vp-tdh immunoglobulin. the molecular weight of nag-rtdh was estimated as 18,500, similar to that of vp-tdh, as judged by sodium dodecyl sulfate slab gel electrophoresis, but its charge or molecul ...	1986	3957429
purification and characterization of vibrio cholerae non-o1 heat-stable enterotoxin.	a toxin which causes rapid fluid accumulation in a suckling mouse assay and which was produced by vibrio cholerae non-o1 was investigated. the toxin was purified from the culture supernatant of v. cholerae non-o1 (strain a-5) by ammonium sulfate fractionation, hydroxyapatite treatment, ethanol extraction, column chromatographies on sp-sephadex c-50 and deae-sephadex a-25, and high-pressure liquid chromatography on a lichrosorb rp-8 column. about 1.4 x 10(5)-fold purification was achieved, with a ...	1986	3957432
relationship between maternal vitamins b2 and b6 status and the levels of these vitamins in milk at different stages of lactation. a study in a low-income group of indian women.	riboflavin and vitamin b6 status of mothers from a low-income group were assessed by erythrocyte glutathione reductase activation and erythrocyte aspartate aminotransferase activation tests respectively, at different stages of lactation. levels of these vitamins in milk were also measured. the majority of the women had biochemical evidence of vitamins b2 and b6 deficiency. there was a bizarre reduction in erythrocyte glutathione reductase activation from 6 to 30 d post partum. levels of riboflav ...	1986	3957716
isoeffect curves for radiation-induced myocardial damage.		1986	3957741
urinary cortisol in the assessment of pituitary-adrenal function: utility of 24-hour and spot determinations.	to more conveniently assess dynamic changes in the biologically active fraction of cortisol, we measured cortisol in 1-h urine samples obtained from 0700-0800 and from 2200-2300 h. in 20 normal subjects, morning 1-h urinary cortisol levels were 78 +/- 36 ng/mg creatinine (mean +/- sd), whereas levels from 2200-2300 h were 22 +/- 12 ng/mg creatinine, demonstrating diurnal variability. in 14 patients with cushing's syndrome, mean morning urinary cortisol was elevated (207 +/- 176 ng/mg creatinine) ...	1986	3958132
persistence of cholera in the united states: isolation of vibrio cholerae o1 from a patient with diarrhea in maryland.	a case of cholera was identified in baltimore county, md., in october 1984. the vibrio cholerae o1 isolate from the patient was hemolytic, biotype el tor, serotype inaba, and was toxigenic by the y-1 adrenal cell assay; on southern blot analysis, the strain had a unique hindiii restriction site in the cholera toxin gene identical to that of other u.s. v. cholerae o1 isolates. two days before he became ill, the patient had eaten meat from crabs harvested along the texas coast.	1986	3958151
multinuclear approach to nuclear magnetic resonance investigations in tissue with heteronuclei: 14n, 35cl, 39k.		1986	3960139
covalent binding of styrene oxide to amino acids, human serum proteins and hemoglobin.		1986	3960869
[significance of atherosclerotic risk factors in the development of preatherosclerotic changes in the human aorta].	a study of the effect of the main risk factors of atherosclerosis in coronary patients on the prevalence of lipoidosis and prelipid changes in the aortal intima showed a week interrelationship between the studied risk factors and prevalence of lipoidosis. a noticeable significant interrelationship of the prevalence of prelipid changes in the aortal intima, patient's age, the iri level and alpha-xc concentration in the blood plasma was established. an equation was described. a nomogram was presen ...	1986	3961688
in vitro production of anti-influenza virus antibody after simultaneous administration of h3n2 and h1n1 cold-adapted vaccines in seronegative children.	sixteen doubly seronegative (h3n2 and h1n1) young children were recently enrolled in a study of live, attenuated cold-adapted influenza a vaccines. twelve children received simultaneously h3n2 and h1n1 live, attenuated influenza vaccine intranasally and four received saline as placebo controls. peripheral blood lymphocytes (pbl) were obtained sequentially from all children and the in vitro production of anti-influenza h3n2 and h1n1 antibody from unstimulated and influenza stimulated cells was me ...	1986	3962451
the effect of needle stimulation of acupuncture loci tienshu (st-25) chung-wan (cv-12) on the immune response in sensitized mice against experimental cholera.	the effects of stimulation of acupuncture loci from tien-shu (st-25) piercing through chung-wan (cv-12) on the leukocytes and immune response were assessed in mice (that is, the anatomical equivalent of these loci of human acupuncture points). the leukocyte count increased and reached its highest level two hours after needling, then restored to normal level 24 hours later both in normal mice and immunized mice. statistical analysis showed no significant variation on the lymphocyte/total leukocyt ...	1986	3962918
effects of arterial ph and hco-3 concentration on hco-3 secretion in the isolated blood-perfused dog pancreas.	we examined the effects of plasma ph and plasma hco-3 concentration on pancreatic hco-3 secretion in resting state and in stimulated state by secretin, pancreozymin or dopamine, using the isolated blood-perfused dog pancreas. secretin- or pancreozymin-induced hco-3 secretion was sensitive to changes in both plasma ph and hco-3 concentration. on the other hand, neither plasma ph nor plasma hco-3 concentration affected dopamine-induced hco-3 secretion and spontaneous hco-3 secretion. these results ...	1986	3963941
synthesis and hypoglycemic activity of 7,8-dihydro-6h-thiopyrano[3,2-d]pyrimidine derivatives and related compounds.		1986	3829149
isolation of non-01 v. cholerae from ear tracts.		1986	3803541
ultrastructural image of the cerebral cortex of rats exposed to hypobaric altitude hypoxia. ii. the effect of prenatal hypoxia on development of the neuroglia cells and capillaries.		1986	3804097
inhibitory effect of nifedipine on cholecystokinin-induced gallbladder contraction in man.	a prospective study was performed on seven male and eight female patients with normal hepatobiliary findings to investigate the effect of 20 mg nifedipine on cholecystokinin-induced gallbladder contraction. each patient received 1 idu (ivy dog unit) cholecystokinin per kg body weight intravenously on two consecutive days, with additional administration of 20 mg nifedipine sublingually on the second day. gallbladder volumes were assessed by ultrasonography over a period of 25 minutes. cholecystok ...	1986	3804183
[studies on the synthesis of salicylamides and alkamine salts of niclosamide and their activities against schistosoma japonicum and oncomelania].		1986	3591335
investigation of cholera acquired from the riverine environment in queensland.	since february 1977, five patients with cholera apparently acquired the infection from the riverine environment in queensland. a total of 13 rivers have now yielded at least one isolate of vibrio cholerae 01 biovar el tor. investigations indicate that the organism, including toxigenic strains, can survive and multiply in the riverine environment. no human or animal reservoirs and no ecological niches were identified and no route of importation or dissemination of the organism was discovered. the ...	1986	3587092
radiologic forensic investigation of fatal gunshot wounds.	the interested radiologist can be a valuable consultant in the investigation of fatal gunshot wounds as well as in the identification of human remains.	1986	3685503
characterization of non-01 vibrio cholerae and v. mimicus isolated from fresh water in bangladesh.		1986	3668192
treatment of experimental hydrofluoric acid burns.		1986	3648026
selection of hypotoxigenic vibrio cholerae population by human intestinal bacteria in gnotobiotic mice.	strains of human intestinal bacteria when cultured with vibrio cholerae 569b, classical inaba, exerted either inhibitory or promotive effects on cholera toxin production. an inhibitory strain of bacteroides fragilis was orally given to germfree mice a week before vibrio administration. in vibrio-monoassociated mice, the toxin production of most vibrios isolated from feces was as high as the inocula, while in bacteroides-vibrio-diassociated mice toxin production decreased starting on day 3 after ...	1986	3617104
morbidity and mortality in a diarrhoeal diseases hospital in bangladesh.	records of all patients who were admitted to or who died in dhaka hospital of the international centre for diarrhoeal disease research, bangladesh (icddr,b) between july 1980 and 30th june 1981 were reviewed to identify epidemiological characters associated with in-hospital diarrhoeal diseases-related deaths. information on aetiological agents, age, sex, major complications, nutritional status and level of dehydration were analysed. over the one-year period, 3251 patients were admitted to the me ...	1986	3603613
the biotransformation of thioridazine to thioridazine 5-sulfoxide stereoisomers in phenobarbital induced rats.	the disposition and urinary elimination of thioridazine 5-sulfoxide (ring sulfoxide) following subacute administration of thioridazine was investigated in control and phenobarbital (pb) induced rats. the ring sulfoxide exists as two stereoisomeric pairs, identified by high performance liquid chromatography as fast eluting (rsf) and slow eluting (rss) ring sulfoxide. no major differences were detected in the distribution in serum or tissue of the ring sulfoxide between control and induced animals ...	1986	3582423
membrane activity, antioxidant, antiaggregatory and antihemolytic properties of four calcium channel blockers.	four calcium channel blockers: nifedipine, fendiline, verapamil and diltiazem have been found to possess different affinity to cytomembranes of platelets and erythrocytes. nifedipine bound to proteins of the external site of cytomembranes. verapamil and fendiline possessed affinity to lipids of the cytoplasmic site of the cytomembranes. diltiazem bound practically neither to erythrocyte nor to platelet membranes. all tested compounds were antioxidants, the strongest being fendiline. antiaggregat ...	1986	3562505
[an epidemic of cholera in the city of pemba (mozambique) in 1983].	the purpose of the epidemiological study about 205 suspected hospitalized cases of cholera was to determinate which model of transmission was the most important. from the results, we noticed that direct non-waterborne has been more frequent than waterborne transmission (mainly in the infection of family contacts of index cases). we also found that virulence of the vibrio cholerae el tor responsible was quite close of virulence of the vibrio cholerae classical.	1986	3562999
induction of protective immunity to malaria.		1986	3553798
host-pathogen interaction--new in vitro drug test systems against mycobacterium leprae--possibilities and limitations.		1986	3553811
acute hepatic failure due to plasmodium falciparum liver injury.	nine patients with acute liver failure due to plasmodium falciparum liver injury admitted to the rajgarhia liver unit of the all-india institute of medical sciences during 1982-84 are presented. the liver was palpable in all the patients, and eight had splenomegaly. investigations revealed mild to moderate abnormality in liver function tests. all were negative for the markers of acute infection due to hepatitis a and b viruses. blood film examination showed p. falciparum alone in seven and along ...	1986	3553821
detection of t. cruzi antigens reactive with igg antibodies from patients with chronic chagas' disease.		1986	3553838
vibriocidal antibody and antibodies to vibrio cholerae lipopolysaccharide, cell-bound haemagglutinin and toxin in thai population.	vibriocidal antibody and antibodies to vibrio cholerae lipopolysaccharide (anti-lps), cell-bound haemagglutinin (anti-cha) and toxin (anti-ct) were determined in thai individuals of various age groups who lived in areas with high (h) and low (l) cholera endemicity. the enzyme-linked immunosorbent assay (elisa) was performed to detect levels of class specific anti-lps, anti-cha and anti-ct. it was found that thai individuals acquired the vibriocidal antibody early in life. fifty percent of indivi ...	1986	3576285
effect of glutaurine on the rat thyroid. electron-microscopic morphological and autoradiographic analysis.		1986	3577330
biochemical and cultural analysis of mycobacterial recombinants obtained by spheroplast fusion.	spheroplast fusion using polyethylene glycol 6000 resulted in mycobacterial recombinants with a frequency of about 10(-3). the parental strains were two carotenoid pigment mutants of mycobacterium aurum which were obtained by chemical mutagenesis of the wild-type strain with n-methyl-n'-nitro-n-nitrosoguanidine. isolation of recombinant clones was based on their pigmentation on löwenstein-jensen medium, and genetically stable recombinants were analysed for their carotenoid pigments and their alp ...	1986	2892462
antigenic recognition of pertussis and cholera toxin by the same antipeptide antibodies.		1986	2892508
identification of a pilus colonization factor that is coordinately regulated with cholera toxin.		1986	2892514
echocardiographic evaluation of left ventricular function during coronary artery angioplasty.	balloon occlusion of a stenotic coronary artery during percutaneous coronary artery angioplasty provides a unique opportunity to study the effect of acute myocardial ischemia on left ventricular (lv) function. simultaneous m-mode and 2-dimensional (2-d) echocardiograms and a 6-lead electrocardiogram were recorded during 20 episodes of coronary artery occlusion and release in 12 patients. no patient had previous myocardial infarction and all had normal lv function by angiography. all patients had ...	1986	2934968
differential depression of hypoglossal nerve activity by alcohol. protection by pretreatment with medroxyprogesterone acetate.	upper airway obstruction during sleep occurs more commonly in men than in women and has been treated with progestational agents with some success. alcohol ingestion exacerbates sleep apnea, and recent studies have established that alcohol depresses the respiratory motor activity to upper airway muscles more than that to the diaphragm, a response pattern that favors upper airway obstruction during inspiration. to investigate the possibility that progesterone provides some protection from this act ...	1986	2935056
photoreactive neurohypophyseal hormone analogs: effects on transport processes in mammalian and amphibian epithelia.	the synthesis of the photoreactive neurohypophyseal hormone analog [3-(p-azidophenylalanine]arginine vasopressin [( phe(p-n3)3]avp is described. [phe(p-n3)3]avp exhibits a rat antidiuretic activity of 173 +/- 18 u/mg and a high binding affinity for the renal v2 vasopressin receptor in bovine kidney; an apparent dissociation constant kd of (1.3 +/- 0.2) x 10(-8) m was determined. in the toad bladder [phe(p-n3)3]avp was the most potent photoreactive vasopressin analog studied to date. it stimulate ...	1986	2936596
platelet deposition on vascular grafts. the accuracy of in vivo quantitation and the significance of in vivo platelet reactivity.	an in vivo platelet imaging system utilizing indium-111-labeled platelets and technetium-99m-labeled red cells was used to serially study and compare platelet deposition on autologous external jugular vein grafts, autologous arterial grafts, polytetrafluoroethylene (gore-tex) and two dacron (meadox and usci) small diameter (4mm) vascular grafts implanted end-to-end in canine carotid and femoral arteries. this method of quantitating platelet deposition was validated by correlating deposition meas ...	1986	2937375
cnbr cleavage of the light chain of human complement factor i and alignment of the fragments.	the light chain and heavy chain of reduced and alkylated human complement factor i were purified by high-pressure gel-permeation chromatography. cnbr cleavage of factor i light chain yielded four major fragments, which were purified by gel filtration. n-terminal sequence analysis of the cnbr-cleavage fragments allowed identification of 200 of the approx. 240 amino acid residues of the light chain. an alignment is proposed, based on sequence analysis of peptides obtained after cleavage at arginin ...	1986	2937401
comparative study of coronary vasodilator and cardiac effects of nitrendipine by use of isolated, blood-perfused dog heart preparations.	the coronary vasodilator and cardiac effects of 3-ethyl-5-methyl-1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl) -3,5-pyridinedicarboxylate (nitrendipine, bay e 5009) were compared in isolated, blood-perfused sinoatrial (sa) node, atrioventricular (av) node and papillary muscle preparations of dogs with i.a. administration. in all preparations nitrendipine increased (coronary) blood flow. in sa node preparations nitrendipine reduced sinus rate but the reduction remained only about 13% of the basal va ...	1986	2937413
cocaine increases natural killer cell activity.	the administration of epinephrine to humans increases natural killer (nk) cell activity and numbers. if endogenous catecholamines regulate nk cells, then their activity should be increased by cocaine, an agent that potentiates endogenous catecholamines. we investigated the in vivo effect of cocaine on nk cell activity and on the distribution of lymphocyte subsets, including nk cells. intravenous cocaine (0.6 mg/kg) produced a three- to fourfold increase in nk cell activity in peripheral blood. t ...	1986	2937807
plasmid-mediated changes in virulence of vibrio cholerae.	the effects of several plasmids, including cloning vectors and r factors, on the virulence of vibrio cholerae ca401r were determined by measuring the dose-related diarrheal response in orally challenged infant mice. the plasmids were also examined for their effects on the colonization ability of strain ca401r by joint infection experiments with a spectinomycin-resistant ca401 strain as an internal standard. one v. cholerae r factor, pvh2, enhanced the diarrheal response, while r factors rts1 and ...	1986	2939028
quantification of sperm motility by a turbidimetric assay. correlation to cellular respiration.	the turbidimetric method according to sokoloski et al. (fertil. steril. 28, 1337-1341, 1977) was used for quantitative measurement of the motility of washed bovine spermatozoa. the method is based on the registration of the increase in absorbance (a538) which is produced by spermatozoa entering the light path after run from the bottom of a photometer cuvette. this increase in absorbance is dependent on the amount of motile cells and their average velocity. the increase in absorbance related to t ...	1986	2939829
complement, membrane glycoproteins, and complement receptors: their role in regulation of the immune response.	to determine the effect of complement on the normal antibody response we studied seven patients with genetically determined complement component deficiencies, guinea pigs deficient of c4 and c2, respectively, and two patients whose neutrophils and monocytes lack the c3bi receptor. patients deficient of early complement components (c4, c2, c3) have abnormal antibody responses to the t-cell-dependent antigen, bacteriophage phi x 174. complement-deficient guinea pigs (c4, c2) produce less antibody ...	1986	2941194
biological properties of htlv-iii/lav: a possible pathway of natural infection in vivo.		1986	2950906
different neuronal location of [3h]sch 23390 binding sites in pars reticulata and pars compacta of the substantia nigra in the rat.	the precise neuronal localization of d1 receptors in the substantia nigra has been studied autoradiographically in the rat by measuring the alterations of [3h]sch 23390 binding site densities in this brain area after 6-hydroxydopamine (6-ohda) induced destruction of nigrostriatal dopaminergic neurons and after ibotenate-induced lesion of striatal afferents. 6-ohda-induced nigral lesion provoked a total loss of [3h]sch 23390 binding sites in the pars compacta and pars lateralis (but not in the pa ...	1986	2950341
ultrastructural changes in the sarcoplasmic reticulum in acute myocardial ischemia.	we investigated the morphologic changes in the sarcoplasmic reticulum (sr) in acute myocardial ischemia, induced by occlusion of the coronary artery in the canine heart, by freeze-fracture of sr in situ and in vitro, and they were compared to the alterations in ca++-stimulated atpase activity and protein composition of the isolated sr. both sr in situ and the isolated sr exhibited typical intramembranous particles with diameters of 70 to 90 a in freeze-fracture replicas. the intramembranous part ...	1986	2948034
dose-related effects of synthetic human beta-endorphin and naloxone on fed gastrointestinal motility.	in humans, plasma beta-endorphin levels rise during application of acute stressful stimuli (vertigo, cold pain, and transcutaneous electrical stimulation) that induce gut motor disturbances. whereas it is possible that circulating beta-endorphin participates in the mediation of these central effects on gut motility, its role cannot be established solely on the basis of changes in plasma levels. therefore, we designed the present study to investigate 1) the dose-related effects of intravenous syn ...	1986	2942039
the (ca2+ + mg2+)-stimulated atpase of the rat parotid endoplasmic reticulum.	a membrane fraction enriched in endoplasmic reticulum was prepared from rat parotid glands by using sucrose-gradient centrifugation. the fraction showed a 10-fold increase in specific activity of nadph: cytochrome c reductase activity over that of tissue homogenates and minimal contamination with plasma membranes or mitochondria. the endoplasmic reticulum fraction possessed both mg2+ -stimulated atpase as well as ca2+, mg2+-atpase [( ca2+ + mg2+)-stimulated atpase]activity. the ca2+, mg2+-atpase ...	1986	2943271
effects of cadmium on the uptake of dopamine and norepinephrine in rat brain synaptosomes.		1986	2943340
effect of boiled-rice feeding in childhood cholera on clinical outcome.	forty-eight children, aged 2-5 years, presenting with watery diarrhoea of less than 48 h duration at home prior to hospitalization, were admitted into a randomized controlled clinical trial, 24 children being treated during the first 24 h of admission with oral rehydration solution (ors) alone and 24 children being given 'ors plus boiled-rice feeding'. the latter group received boiled-rice to supply at least 55 kcal/kg/d (about 150 g boiled-rice per feed, given four times daily). vibrio cholerae ...	1986	2943703
preliminary observations on lungworms in farmed and feral red deer (cervus elaphus) in denmark.	deer farming is on its increase in denmark. based on experiences from other countries, lungworm infection is expected to cause severe problems in farmed deer. therefore, in the present paper, which is the first one to deal with pathogens in danish deer farms, the faecal larval excretion in red deer is examined.	1986	2944072
merits and pitfalls in morphological assessment of cardiac growth.	the basic principles of quantitative morphological analysis of myocardial tissue are presented. particular attention is paid to the effects of stimulated cardiac growth on the relationship between cell size and various indices of myocardial capillary supply in pressure-overloaded rat hearts. the importance of adequate sampling and of the number of measurements is stressed with respect to reliable estimation of the mean values but even more for assessment of variability of the measured data. when ...	1986	2944767
merits and pitfalls in morphological assessment of cardiac growth.	the basic principles of quantitative morphological analysis of myocardial tissue are presented. particular attention is paid to the effects of stimulated cardiac growth on the relationship between cell size and various indices of myocardial capillary supply in pressure-overloaded rat hearts. the importance of adequate sampling and of the number of measurements is stressed with respect to reliable estimation of the mean values but even more for assessment of variability of the measured data. when ...	1986	2944767
h-2-linked genes determine the level of the primary in vitro anti-mls response.	the level of cell proliferation and interleukin-2 (il-2) production observed in an anti-mls mixed lymphocyte reaction between spleen cells from h-2 compatible, mls incompatible mouse strains is determined by the h-2 haplotype of the mouse combination. thus, while akr (h-2k) spleen cells stimulated strong mlsa responses in h-2k responder cells, akr-h-2b spleen cells stimulated no or negligible mlsa responses in responder cells from h-2b mouse strains. this effect was observed at the levels of il- ...	1986	2944823
interaction studies in mice of sch 29851, a potential non-sedating antihistamine, with commonly used therapeutic agents.	sch 29851 was studied for its ability to interact with selected drugs used in man. the non-sedating antihistamine terfenadine and the sedating antihistamine diphenhydramine were also studied for comparison. sch 29851 at 80 mg/kg po in mice, a dose about 50 times its ed50 for blocking histamine-induced paw edema in the same species, potentiated the anticonvulsant effects of diazepam. at the high dose of 320 mg/kg po, about 80 times its ed50 for antihistamine effects, sch 29851 potentiated the abi ...	1986	2945410
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
structure-activity relationships of sex steroid analogs determined in vitro in a thyroid hormone-responsive membrane ca2+-atpase model.	physiological concentrations of l-t4 were found previously to stimulate ca2+-atpase activity in vitro in reticulocyte membranes from female rabbits and to inhibit this enzyme in reticulocyte membranes from males. in these previous studies, preincubation of intact cells or ghosts with testosterone (5 x 10(-11) m) converted female-source reticulocyte membranes to male-type responsiveness to thyroid hormone (inhibition of ca2+-atpase activity). preincubation of reticulocyte membranes with 17 beta-e ...	1986	2946570
biosynthesis of sulfated asparagine-linked oligosaccharides on bovine lutropin.	the asparagine-linked oligosaccharides on bovine lutropin (blh) are unusual, containing galnac and sulfate but no galactose or sialic acid. oligosaccharides from metabolically radiolabeled or purified blh consist of non- (neutral), mono- (s-1), and di- (s-2) sulfated structures. we have previously shown that s-2 is a complex type oligosaccharide bearing two peripheral branches with the sequence so4----galnac----glcnac attached to a typical man3glcnac2 core (green, e.d., van halbeek, h., boime, i ...	1986	2946684
class ii major histocompatibility complex and organ specific autoimmunity in man.	t helper lymphocytes only recognize the antigen to which they are directed if it is 'presented' to them by a cell expressing the same class ii major histocompatibility complex (mhc) antigen as themselves. since the target cells of most organ specific autoimmune diseases do not normally express class ii mhc proteins on their surface, any cell specific surface antigens they possess may never have been presented, and therefore immunological tolerance to them may not exist. the target cells in sever ...	1986	2946837
possible role of helper and cytolytic t cells in mycobacterial infections.		1986	2952852
comparative investigation of human endomyocardial biopsy.		1986	2979163
[effect of culture media on the production of cholera enterotoxin].		1986	3079511
[comparative study of the production of enterotoxin by vibrio cholerae 01 and vibrio cholerae non-01].		1986	3079514
rat pulmonary artery wall injury by chronic intermittent infusions of escherichia coli endotoxin. obliterative vasculitis and vascular occlusion.	to examine the effect of intermittent endotoxemia on rat pulmonary artery structure and hemodynamic function we infused purified escherichia coli endotoxin on four occasions over 3 weeks (at 7-day intervals), through an indwelling catheter placed in the external jugular vein. the fourth infusion of endotoxin was associated with widespread but focal alveolar consolidation, reduced perfusion of small pulmonary arteries by lumen occlusion and obliteration, pulmonary vascular wall injury, and a peri ...	1986	3079547
rat pulmonary artery wall injury by chronic intermittent infusions of escherichia coli endotoxin. obliterative vasculitis and vascular occlusion.	to examine the effect of intermittent endotoxemia on rat pulmonary artery structure and hemodynamic function we infused purified escherichia coli endotoxin on four occasions over 3 weeks (at 7-day intervals), through an indwelling catheter placed in the external jugular vein. the fourth infusion of endotoxin was associated with widespread but focal alveolar consolidation, reduced perfusion of small pulmonary arteries by lumen occlusion and obliteration, pulmonary vascular wall injury, and a peri ...	1986	3079547
lipopolysaccharide structures in enterobacteriaceae, pseudomonas aeruginosa, and vibrio cholerae are immunologically related to campylobacter spp.	to determine whether lipopolysaccharide (lps) structures of campylobacter species are immunologically related to those of 11 other gram-negative organisms, we immunoblotted from polyacrylamide gels the lps of these strains with immune rabbit serum raised against six campylobacter jejuni strains and two campylobacter fetus strains. the lps studied were from salmonella minnesota wild type and ra to re mutants, salmonella typhi, escherichia coli, yersinia enterocolitica, vibrio cholerae, and pseudo ...	1986	3079730
antagonism of antigen-induced contraction of isolated guinea-pig trachea by 5-lipoxygenase inhibitors.	tracheal strips were obtained from guinea pigs actively sensitized to chicken ovalbumin (oa). oa-induced contractile responses, obtained by cumulative log addition, were found to be comparable both temporally and in magnitude with responses obtained by single addition of oa. the concentration-response curve obtained by cumulative addition of oa was partially inhibited by pretreatment of tissues with chlorpheniramine. on tracheal spirals treated with chlorpheniramine, indomethacin enhanced oa-ind ...	1986	3079739
evidence that pulsatile follicle-stimulating hormone secretion is independent of endogenous luteinizing hormone-releasing hormone.	although lhrh can stimulate the release of both lh and fsh from the pituitary, there are a number of instances in which the secretion of lh and fsh are divergent. previous studies from our laboratory have indicated that pulsatile lh and fsh secretion are independently regulated by gonadal factors. we have, therefore, reexamined the role of lhrh in regulating pulsatile gonadotropin secretion by evaluating the effect of passive lhrh immunoneutralization on lh and fsh secretion in castrate adult ma ...	1986	3080310
identity of hemolysins produced by vibrio cholerae non-o1 and v. cholerae o1, biotype el tor.	hemolysins purified from non-o1 vibrio cholerae (non-o1 hemolysin) and a vibrio cholerae o1, biotype el tor (el tor hemolysin) were investigated for their homology. the hemolysins were isolated from the culture supernatant fluids by ammonium sulfate precipitation and gel filtration on sephadex g-100 columns. the purified hemolysins gave single bands with an identical mobility on conventional polyacrylamide gel disc electrophoresis. the molecular weights of the non-o1 and el tor hemolysins were e ...	1986	3081448