Publications

microbiological study on early plaque formation in partial denture wearers.		1987	3476293
comparative biodistributions and rates of catabolism of radiolabelled anti-cea monoclonal antibody and control immunoglobulin in nude mice with human tumour xenografts showing specific antibody localization.	the rate of catabolism of a radiolabelled monoclonal anti-cea antibody has been compared with that of control igg1 in control nude mice and in mice with cea producing xenografts and antigen negative xenografts. the rate of catabolism of antibody, but not of igg, was increased 3-4 fold in mice with xenografts localizing the antibody, but not in mice with antigen negative tumours. there was no evidence of immune complex formation and/or clearance of antibody from the serum of xenografted mice and ...	1987	3478194
inducible reactivation of uv-irradiated cholera phage e5 in vibrio cholerae mak757.	the survival of uv-irradiated cholera phage e5 was found to increase when the host cells, vibrio cholerae mak757, were exposed to a low dose of uv irradiation before phage infection (weigle reactivation), indicating the existence of a uv-inducible dna repair pathway (sos repair) in v. cholerae mak757. the induction signal generated by uv irradiation was transient in nature and lasted about 20-30 min at 37 degrees c. maximal weigle reactivation of the phage was obtained when the host cells were i ...	1987	3478551
conformation of protein secreted across bacterial outer membranes: a study of enterotoxin translocation from vibrio cholerae.	the secretion of enterotoxin by vibrio cholerae is punctuated by the transient entry of the toxin subunits into the periplasm. in this paper, we show that the subunits oligomerize into an assembled holotoxin within the periplasm prior to their secretion across the outer membrane. the rate of toxin assembly was studied by pulse-labeling cells with [35s]-methionine and then monitoring the turnover of radiolabeled subunits as they assembled within the periplasm. the subunits entered the periplasm a ...	1987	3478701
the sensitivity of gram-negative and gram-positive bacteria to ofloxacin.	the in vitro antimicrobial activity of ofloxacin, a new fluorinated quinolone, was evaluated against 165 gram-negative rods, both fermentative and non-fermentative, and against 57 gram-positive strains (coagulase-positive and -negative staphylococci both methicillin-resistant and -susceptible, and streptococcus faecalis). minimal inhibitory concentrations were determined by using the macrodilution test and the activity was compared with nalidixic acid, norfloxacin, ampicillin, piperacillin, ceft ...	1987	3480796
endotoxins in poultry production and human lung reactions.		1987	3480834
effects of dietary fish oil on renal insufficiency in rats with subtotal nephrectomy.	we studied the effects of fish oil on the progression of renal insufficiency in rats with subtotal nephrectomy. five weeks after a 1-2/3 nephrectomy, sixteen rats were fed two different diets which differed only in fat composition. lipid in the control diet was primarily beef tallow; that of the experimental diet, menhaden oil. fish oil-fed rats had significant increases in plasma creatinines, decreases in urinary pge2 and accelerated death rates. an additional twelve rats underwent 1-1/3 nephre ...	1987	3480973
the development of mandibular molar tooth germs isografted in the mouse spleen.	tooth germs taken from 13-day-old embryonic mice and isografted in the spleen of adult mice were examined by light and electron microscopy. well-organized tooth structures from the early cap stage to fully developed and mineralized mature teeth were obtained up to day 60 after transplantation. germs on day 2 were similar to those prior to the onset of grafting but reached the late cap stage of development on day 4. on day 6, enamel and dentine formation were initiated and inner enamel epithelium ...	1987	3482151
[histopathological changes in dental pulp in rats after irradiation of nd:yag laser].		1987	3482212
essays on the art of caring in nursing: i. the human spirit in stress management.		1987	3509708
t cell subsets in the blast cell population in phytohemagglutinin-stimulated peripheral blood in vitro and in rheumatoid arthritis synovial fluid in vivo.	the immunomodulatory t4/t8 ratio was studied in the total and activated lymphocyte populations by a method combining visualisation of 3h-thymidine incorporating blasts with autoradiography (ar) with simultaneous identification of the respective lymphocyte subsets using monoclonal antibodies in avidin-biotin-peroxidase complex (abc) staining. in rheumatoid arthritis synovial fluid the activated t4/t8 ratio (calculated from t cells in the s phase of the cell cycle) was significantly different from ...	1987	3497421
trimethoprim used for selective decontamination of the digestive tract in rats: possible route of excretion.	selective elimination of enterobacteriaceae species from the digestive tract of rats has been accomplished by oral treatment with trimethoprim (235 mg/kg body weight/day) within 6 days. in the present study it was investigated whether this elimination was mainly due to antimicrobial activity of trimethoprim excreted with the gastrointestinal mucus or mainly by non-absorbed trimethoprim in the lumen contents. by means of whole gut irrigation (wgi) the lumen contents were washed out, followed by m ...	1987	3497436
difference in monoamine oxidase b activity between c57 black and albino nmri mouse strains may explain differential effects of the neurotoxin mptp.	monoamine oxidase b (mao-b) is the key enzyme in the conversion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(mptp) to n-methyl-4-phenyl-pyridinium ion (mpp+) which causes degeneration of dopaminergic nigral neurons. using a histochemical tetrazolium method for mao-b with tyramine as substrate and chlorgyline for the inhibition of mao-a, black c57 mice were found to have a higher brain mao-b activity than similar aged albino nmri mice. the difference, which was in general density rather than d ...	1987	3498133
human immunodeficiency virus infection in hemophilia patients: a 4-year prospective study.		1987	3499114
causes of death in diarrhoeal diseases after rehydration therapy: an autopsy study of 140 patients in bangladesh.	the cause of death (besides dehydration) for 140 diarrhoeal patients who died in hospital following rehydration was determined by autopsy examination. children under 5 years comprised 74% of the patients. diarrhoeal pathogens were identified as shigella spp. in 27%, enterotoxigenic escherichia coli in 17%, entamoeba histolytica in 16%, campylobacter jejuni in 12%, salmonella spp. in 4%, vibrio cholerae in 4%, and giardia lambliain 4% of cases. the most frequent underlying causes of death were co ...	1987	3499247
vaccination against cutaneous leishmaniasis in a murine model. ii. immunologic properties of protective and nonprotective subfractions of soluble promastigote extract.	we have previously demonstrated that balb/c mice can be protected against a fatal infection with leishmania major by i.p. immunization with a soluble leishmanial antigen (sla) preparation in conjunction with the adjuvant, corynebacterium parvum (cp). in this study, sla was separated into nine distinct fractions by anion exchange liquid chromatography, and the fractions were analyzed for their ability to stimulate t cells obtained from immunized mice, to be recognized by vaccine-induced antibodie ...	1987	3499465
in vitro radiosensitivity of human fresh t-lymphocytes by colony formation assay using pha and recombinant interleukin-2.		1987	3501012
chronic ethanol consumption reduces [3h]inositol (1,4,5) trisphosphate specific binding in mouse cerebellar membrane fragments.	[3h]in(1,4,5)p3 specific binding was determined in membrane fragments from various brain regions of adult male c57/bl mice. [3h]in(1,4,5)p3 specific binding was at least 10 times higher in cerebellum than in either striatum, cerebral cortex, hippocampus, or midbrain. ethanol added in vitro up to 500 mm to cerebellar membrane fragments of control mice had no significant effect on [3h]in(1,4,5)p3 specific binding. in contrast, the maximal number of binding sites (bmax) for [3h]in(1,4,5)p3 was sign ...	1987	3501055
biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro.	osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ...	1987	3502681
biphasic effects of epidermal growth factor on bone nodule formation by isolated rat calvaria cells in vitro.	osteoblast-like cells isolated from fetal rat calvaria (rc) form three-dimensional nodules in vitro having the morphological and immunohistochemical characteristics of bone. when administered continually over 21 days of culture, egf caused a dose-related inhibition of nodule formation, as well as a reduction in the proportion of mineralized nodules, at concentrations between 10(-12) to 10(-7) m. the same conditions caused an increase in both cell proliferation and saturation density in the cultu ...	1987	3502681
comparative gene mapping: a valuable new tool for mammalian developmental studies.	technological advances in the 1970s encouraged the mapping of homologous gene loci in different mammalian species, including mouse and man. one hundred eighty-five homologous loci have now been mapped in these two species. conservation of linkage is sufficient to identify substantial segments of the two genomes that have been left intact since their divergence from a common ancestor. the recognition of these conserved segments allows experimental manipulation of mouse chromosomes or chromosomal ...	1987	3502975
campylobacter diarrhea in an adult mouse model.	an adult mouse (18-20 g) model was developed for studying the pathogenesis of campylobacter isolates. iron-loaded balb/c mice given 10(8)-10(9) campylobacter colony forming units by intraperitoneal injection developed a severe mucoid diarrhea within 4 h. severe diarrhea, consisting of unformed stools containing blood, mucus, and fecal leukocytes, persisted for 24 h. diarrheal symptoms in surviving mice resolved gradually; no diarrhea was observed 5 days after inoculation. mice not pretreated wit ...	1987	3504219
tissue damage in vitamin e deficient rats is not detected by expired ethane and pentane.	despite evidence for tissue damage in vitamin e deficient rats, no discernible difference in ethane and pentane production was apparent compared with controls. it is suggested that other sources of hydrocarbons may mask hydrocarbon production from peroxidation of polyunsaturated fatty acid components of cell membranes, or that tissue damage occurs before peroxidation of the polyunsaturated fatty acids.	1987	3506893
susceptibility of epididymal boar sperm to cold shock and protective action of phosphatidylcholine.	rapidly cooling (cold shocking) washed cauda boar sperm irreversibly reduced motility and respiration and greatly increased the uptake of 45ca2+; the plasma membranes were removed and the acrosomes detached from nuclei. the motility, respiration, and calcium uptake of the less mature caput sperm were largely unaffected; and there was little damage to the ultrastructure. this indicates that boar sperm becomes less resistant to cold shock as they mature in the epididymis. the oxygen uptake, glucos ...	1987	3507358
susceptibility of epididymal boar sperm to cold shock and protective action of phosphatidylcholine.	rapidly cooling (cold shocking) washed cauda boar sperm irreversibly reduced motility and respiration and greatly increased the uptake of 45ca2+; the plasma membranes were removed and the acrosomes detached from nuclei. the motility, respiration, and calcium uptake of the less mature caput sperm were largely unaffected; and there was little damage to the ultrastructure. this indicates that boar sperm becomes less resistant to cold shock as they mature in the epididymis. the oxygen uptake, glucos ...	1987	3507358
cholera in a pacific island.		1987	3507429
[chemical properties and biocompatibility of ca(oh)2].		1987	3507992
modulation of 3-hydroxy-3-methylglutaryl-coa reductase by changes in microsomal cholesterol content or phospholipid composition.	microsomal preparations from normal human skin fibroblasts were used to investigate the role of free cholesterol in the endoplasmic reticulum in the control of cholesterol biosynthesis by regulation of 3-hydroxy-3-methylglutaryl-coenzyme a reductase (nadph) [(s)-mevalonate:nadp+ oxidoreductase (coa-acylating), ec 1.1.1.34]. controlled changes in the cholesterol/phospholipid ratio of microsomes were introduced either in intact cells by incubating fibroblast cultures with whole serum or lipoprotei ...	1987	3467343
modulation of 3-hydroxy-3-methylglutaryl-coa reductase by changes in microsomal cholesterol content or phospholipid composition.	microsomal preparations from normal human skin fibroblasts were used to investigate the role of free cholesterol in the endoplasmic reticulum in the control of cholesterol biosynthesis by regulation of 3-hydroxy-3-methylglutaryl-coenzyme a reductase (nadph) [(s)-mevalonate:nadp+ oxidoreductase (coa-acylating), ec 1.1.1.34]. controlled changes in the cholesterol/phospholipid ratio of microsomes were introduced either in intact cells by incubating fibroblast cultures with whole serum or lipoprotei ...	1987	3467343
surface charge of resident, elicited, and activated mouse peritoneal macrophages.	the surface charge of resident, thioglycollate-elicited, and trypanosoma cruzi-activated mouse peritoneal macrophages was analyzed using cell electrophoresis. all macrophages had a net negative surface charge. activated macrophages had a lower zeta potential and a higher isoelectrophoretic point than resident and elicited macrophages. the populations of resident, elicited, and activated macrophages were heterogeneous in terms of surface charge. the analysis of the effect of the ph of the solutio ...	1987	3468193
monoclonal igm antibodies that inhibit primary moloney murine sarcoma growth.	monoclonal igm antibodies with specificity for moloney murine sarcoma virus (m-musv)-moloney murine leukemia virus (m-mulv) from two hybridoma clones have been isolated and characterized. the monoclonal antibodies have specificity for a cytoplasmic and cell surface friend-moloney-rauscher group-specific antigen. immunoelectron microscopy revealed antibody binding to the surface of virus-expressing cells but not to the budding virus particles. treatment of m-musv-injected mice with monoclonal igm ...	1987	3469466
binding of [3h]skf 38393 to dopamine d-1 receptors in rat striatum in vitro; estimation of receptor molecular size by radiation inactivation.	[3h]skf 38393 (2,3,4,5-tetrahydro-7,8-dihydroxy-1-phenyl-1h-3-benzazepine) binds with high affinity to 3,4-dihydroxyphenylethylamine (dopamine) d-1 receptors in rat striatum in vitro (kd = 7 and 14 nm in nonfrozen and frozen striatum, respectively). the number of binding sites (bmax) was approximately 80.0 pmol/g of original tissue, a value similar to the bmax for the dopamine d-1 antagonist sch 23390. nondisplaceable [3h]skf 38393 binding was approximately 45% of total binding. irradiation (0-4 ...	1987	3491877
structural characterization of gangliosides from murine t lymphocytes.	mouse spleen cells were prepared from cba/j mice, and t lymphocytes were selectively stimulated with the t cell mitogen concanavalin a and further propagated in the presence of the t cell growth factor interleukin-2. the t cells were metabolically labeled with d-[1-14c]galactose and d[1-14c]glucosamine, and the gangliosides were extracted and purified by deae-sepharose column chromatography. carbohydrate backbone structures of the asialogangliosides, prepared by mild acid hydrolysis, were determ ...	1987	3493135
transient entry of enterotoxin subunits into the periplasm occurs during their secretion from vibrio cholerae.	cholera toxin and heat-labile enterotoxin (lt) are structurally similar oligomeric proteins which are capable of being efficiently secreted from vibrio cholerae. here we report that these proteins transiently enter the periplasm of v. cholerae as they traverse the cell envelope to reach the extracellular milieu. pulse-chase experiments on v. cholerae trh7000 harboring an lt-encoding plasmid revealed that radiolabeled lt a and b subunits entered the periplasm rapidly, followed by their slow efflu ...	1987	3493239
new approaches for the analysis of mast cell maturation, heterogeneity, and function.	mast cells are regarded as potentially critical participants in a wide variety of important biological processes. yet it has been difficult to ascertain the precise contribution of mast cells to many of these reactions. in part, this reflects recognition that different populations of mast cells may vary in phenotype and therefore may express different functions. another problem has been the lack of suitable model systems for identifying and quantitating the mast cell's unique roles in biological ...	1987	3493923
human t hybridoma-derived immunoglobulin-binding factors.	a human t hybridoma was generated lacking demonstrable low-affinity ige surface receptors. this cell line produces spontaneously immunoglobulin-binding factors. the factors have an affinity for human ige and igg as well as for igg from other species. the binding factors were purified by concanavalin a affinity chromatography, an ige immunoaffinity column and sds-page. this purification procedure resulted in five major proteins bands at 13, 15, 30, 60 and less than 150 kilodaltons. as judged by w ...	1987	3494683
[lymphocyte cytotoxicity against autologous tumor cells and the effect of interferon-beta on their activities. (1) evaluation by modification of target tumor cells].	ifn is known to enhance nk activity against cultured cell lines such as k562, but not against frozen autologous tumor cells. in order to obtain increased nk cytotoxicity using ifn-beta, various modifications were performed on autologous tumor cells. ifn-beta induced more enhanced nk cytotoxicity of normal lymphocytes when frozen tumor target cells were cultured for 4-5 days in the medium, or when these cells were treated with vibrio cholerae neuraminidase (vcn). however, in an autologous setting ...	1987	3495240
changes in the concentration of the calcium-binding parvalbumin in cross-reinnervated rat muscles. comparison of biochemical with physiological and histochemical parameters.	the fast extensor digitorum longus (edl) and the slow soleus (sol) muscles were cross-reinnervated in both directions in the rat. during the following transformation of muscle type properties, the expression of the ca2+-binding parvalbumin (parvalbumin, mr = 12,000) was investigated. the combined biochemical, histochemical, and physiological results demonstrated that the amount of parvalbumin decreased in the fast to slow (x-edl) and increased in the slow to fast (x-sol) transformation. alterati ...	1987	3539938
b subunit-whole cell and whole cell-only oral vaccines against cholera: studies on reactogenicity and immunogenicity.	we conducted a randomized trial among persons in rural bangladesh to evaluate the side effects and immunogenicity of orally administered b subunit-killed whole cell (bs-wc) and killed whole cell-only (wc) cholera vaccines and a killed escherichia coli strain k12 placebo proposed for field testing. three doses of bs-wc, wc, e. coli, or a control agent were given with antacid to 1,257 women (aged greater than 15 years) and children (aged to to 15 years). the four groups exhibited no statistically ...	1987	3540139
trace amounts of ganglioside gm1 in human milk inhibit enterotoxins from vibrio cholerae and escherichia coli.	gangliosides were isolated from human milk fat and purified by silica gel column chromatography and high performance liquid chromatography (hplc). low amounts of the ganglioside gm1, detected by high performance thin layer chromatography (hptlc)-immunoassay, were found in all fractions with enterotoxin-inhibitory activity, while fractions without gm1 were inactive. it is concluded that gm1 is responsible for enterotoxin-inhibitory activity in the ganglioside fraction from human milk.	1987	3540506
comparative study of vibrio cholerae non-o1 protease and soluble hemagglutinin with those of vibrio cholerae o1.	protease and soluble hemagglutinating activities produced by a non-o1 vibrio cholerae strain isolated from a patient with diarrhea were compared with similar activities produced by v. cholerae o1. the soluble protease activities were indistinguishable in heat stability, immunodiffusion, inhibition by antiserum, and electrophoretic analysis. on the other hand, the soluble hemagglutinating activities of both strains were not completely identical. the hemagglutinating activity of the non-o1 v. chol ...	1987	3542835
human pituitary tryptase: molecular forms, nh2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates.	a human pituitary-derived serine protease, immunologically identical to human lung tryptase (smith, t. j., hougland, m.w., and johnson, d.a. (1984) j. biol. chem. 259, 11046-11051), was found immunohistochemically to be associated with mast cells present in pituitary connective tissue. western blotting combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated the presence of multiple forms: a major mr 36,300 form and three minor forms with mr 32,400, 33,400, and 34,600. t ...	1987	3543004
human pituitary tryptase: molecular forms, nh2-terminal sequence, immunocytochemical localization, and specificity with prohormone and fluorogenic substrates.	a human pituitary-derived serine protease, immunologically identical to human lung tryptase (smith, t. j., hougland, m.w., and johnson, d.a. (1984) j. biol. chem. 259, 11046-11051), was found immunohistochemically to be associated with mast cells present in pituitary connective tissue. western blotting combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated the presence of multiple forms: a major mr 36,300 form and three minor forms with mr 32,400, 33,400, and 34,600. t ...	1987	3543004
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.	we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...	1987	3543022
phosphorylation, glycosylation, and proteolytic activity of the 52-kd estrogen-induced protein secreted by mcf7 cells.	we have studied the posttranslational modifications of the 52-kd protein, an estrogen-regulated autocrine mitogen secreted by several human breast cancer cells in culture (westley, b., and h. rochefort, 1980, cell, 20:353-362). the secreted 52-kd protein was found to be phosphorylated mostly (94%) on high-mannose n-linked oligosaccharide chains, and mannose-6-phosphate signals were identified. the phosphate signal was totally removed by alkaline phosphatase hydrolysis. the secreted 52-kd protein ...	1987	3543022
evolutionary origin of pathogenic determinants in enterotoxigenic escherichia coli and vibrio cholerae o1.	three families of the evolutionarily related pathogenic determinants in enterotoxigenic escherichia coli and vibrio cholerae o1, a family of cholera enterotoxin (ct) and heat-labile enterotoxin (lt) including ct, lth, and ltp, a family of heat-stable enterotoxin i (sti) including stia and stib, and a family of k88 enteroadhesion fimbriae including k88ab, k88ac, and k88ad were analyzed for synonymous (silent) nucleotide substitutions by using the gene nucleotide sequences of earlier reports and t ...	1987	3546273
predation on rhodnius prolixus (hemiptera: reduviidae) by the spider theridion rufipes (araneida: theridiidae).		1987	3546692
a new elisa test for hiv antibodies using a bacterially produced viral env gene product.	screening tests for antibodies to the human immunodeficiency virus (hiv), based on the indirect elisa principle using viral preparations as antigen, yield a substantial number of false-positive and false-negative results. these failures are due to the lack of certain viral polypeptides or contaminating cellular polypeptides in viral preparations. therefore, the accuracy of the screening tests should be improved by using highly purified, synthetic viral antigens. with establishment of such an eli ...	1987	3547057
lysosomal enzyme replacement in neural tissue by allogeneic bone marrow transplantation following total lymphoid irradiation in canine fucosidosis.		1987	3547950
role of apolipoprotein e in lipoprotein metabolism.		1987	3544762
adult respiratory distress syndrome. a live e coli septic primate model.		1987	3548404
randomized controlled trial of berberine sulfate therapy for diarrhea due to enterotoxigenic escherichia coli and vibrio cholerae.	to evaluate the antisecretory activity of berberine sulfate (bs), we studied 165 adult patients with acute diarrhea due to enterotoxigenic escherichia coli (etec) and vibrio cholerae in randomized controlled trials. in patients with etec diarrhea who received 400 mg of bs in a single oral dose, the mean stool volumes were significantly less than those of the controls during three consecutive 8-hr periods after treatment (p less than .05). at 24 hr after treatment, significantly more patients who ...	1987	3549923
acute radiation-induced vomiting in area postrema-ablated cats.	a dose-response relationship was established in normal unanesthetized cats for emetic incidence, latency to onset of vomiting, and duration of emetic activity over a period of 24 h after whole-body exposure to 60co radiation at selected doses between 7.5 and 90 gy. each episode of vomiting, i.e., retching and expulsion, was recorded oscillographically as its characteristic intrathoracic pressure waveform by means of a catheter inserted some days before into the superior vena cava. the gamma-radi ...	1987	3550870
acute radiation-induced vomiting in area postrema-ablated cats.	a dose-response relationship was established in normal unanesthetized cats for emetic incidence, latency to onset of vomiting, and duration of emetic activity over a period of 24 h after whole-body exposure to 60co radiation at selected doses between 7.5 and 90 gy. each episode of vomiting, i.e., retching and expulsion, was recorded oscillographically as its characteristic intrathoracic pressure waveform by means of a catheter inserted some days before into the superior vena cava. the gamma-radi ...	1987	3550870
antibacterial activity and mechanism of action of 3'-azido-3'-deoxythymidine (bw a509u).	the thymidine analog 3'-azido-3'-deoxythymidine (bw a509u; azidothymidine [azt]) had potent bactericidal activity against many members of the family enterobacteriaceae, including strains of escherichia coli, salmonella typhimurium, klebsiella pneumoniae, shigella flexneri, and enterobacter aerogenes. azt also had bactericidal activity against vibrio cholerae and the fish pathogen vibrio anguillarum. azt had no activity against pseudomonas aeruginosa, gram-positive bacteria, anaerobic bacteria, m ...	1987	3551832
nocardia asteroides as a cause of corneal ulcer. case report.		1987	3551895
failure of klebsiella pneumoniae antibodies to cross-react with peripheral blood mononuclear cells from patients with ankylosing spondylitis.	cross-reactivity between antibodies to 2 strains of klebsiella pneumoniae (k43 and f77) and the peripheral blood lymphocytes of patients with ankylosing spondylitis (as) was examined in 3 separate antibody binding and cytotoxicity assays. using k pneumoniae antisera in a chromium release cytotoxicity assay, we found no difference in the reactions of cells from as patients and those from control subjects. this result contrasts with the results of previous studies. similarly, using an enzyme-linke ...	1987	3551965
extrarenal scintigraphic aspects of kidney transplant scans obtained with 99mtc-dtpa.	in a retrospective study, 300 scans obtained with 99mtc-dtpa of 100 kidney transplant recipients were studied. the aim was to look for extrarenal scintigraphic aspects encountered specifically in renal transplant scintigraphy and to determine whether some of these phenomena could interfere with adequate interpretation. the most important phenomena seen were radioactivity in the uterus and a cold spot in the pelvis minor caused by a urine filled bladder. if the phenomena that can be encountered a ...	1987	3552685
protective efficacy in humans of killed whole-vibrio oral cholera vaccine with and without the b subunit of cholera toxin.	natural protection from cholera is associated with local intestinal antibacterial and antitoxic antibodies, which appear to act synergistically. although current parenteral cholera vaccines offer insufficient protection, new vaccines administered orally have more promise. killed vibrio cholerae, alone or given with the b subunit of cholera toxin, was evaluated in adult volunteers. vaccinees, who received three doses of either vaccine, and unvaccinated controls ingested 10(6) v. cholerae organism ...	1987	3552989
elimination of 131i in meadow voles (microtus pennsylvanicus) measured by whole-body gamma spectrometry.		1987	3553074
human skin chymotrypsin-like proteinase chymase. subcellular localization to mast cell granules and interaction with heparin and other glycosaminoglycans.	the subcellular localization of human skin chymase to mast cell granules was established by immunoelectron microscopy, and binding of chymase to the area of the dermo-epidermal junction, a basement membrane, was demonstrated immunocytochemically in cryosections incubated with purified proteinase prior to immunolabeling. because heparin and heparan sulfate proteoglycans are major constituents of mast cell granules and basement membranes, respectively, the ability of chymase to bind to glycosamino ...	1987	3553192
evaluation of immuno-dot-blot assay for detection of cholera-related enterotoxin antigen in salmonella typhimurium.	twenty-five strains of salmonella typhimurium isolated in india were examined for the presence of cholera/coli-related enterotoxin antigen by a previously described latex particle agglutination test and by a newly developed immuno-dot-blot test using immunopurified goat antibody against the cholera-related enterotoxin isolated from an escherichia coli strain of human origin. the immuno-dot-blot assay could detect 0.02 ng of purified enterotoxin. the amount of toxin antigen detected varied widely ...	1987	3553233
assay for heat-labile escherichia coli enterotoxin using sandwich erythroimmunoassay.	we investigated the possibility of using a sheep erythrocyte-antibody conjugate as reagent in a sandwich erythroimmunoassay (seria) procedure to detect and titrate escherichia coli heat-labile enterotoxin (lt) with the naked eye. in this assay, which is based on the immunological similarity between vibrio cholerae toxin (ct) and lt, rabbit anti-ct igg was used as immunosorbent, and sheep erythrocytes, sensitized with the rabbit anti-ct antibodies, were used as indicator. the sensitivity of the t ...	1987	3553884
mutation frequency decline in escherichia coli b/r after mutagenesis with ethyl methanesulfonate.	nonsense-defective auxotrophic strains of escherichia coli b/r were used to study mutation frequency decline (mfd) after mutagenesis with ethyl methanesulfonate (ems). the mutation frequencies for prototrophic revertants that were either converted or de novo glutamine trna suppressor mutations declined as treated auxotrophic parental cells were incubated with glucose but without required amino acids (a condition typically producing mfd). the decline for converted suppressor mutations was more ra ...	1987	3553916
the morphology and distribution of serotonin-like immunoreactive fibers in the cat dorsal column nuclei.	fibers showing serotonin-like immunoreactivity (5-ht-li) are demonstrated in the gracile, cuneate and external cuneate nuclei of the cat using avidin-biotinylated horseradish peroxidase and indirect fluorescence immunohistochemical methods. 5-ht-li fibers are located in all cytoarchitectural subdivisions of the gracile and cuneate nuclei but are restricted to the medial half of the external cuneate nucleus. in all nuclei, 5-ht-li fibers consist of long, varicose strands showing a wide range in t ...	1987	3554006
calcitonin gene-related peptide in the ventral tegmental area: selective modulation of prefrontal cortical dopamine metabolism.	the distribution of calcitonin gene-related peptide (cgrp) in the a10 dopamine (da) cell group region of the ventral tegmental area (vta) of the rat was examined using immunohistochemical techniques. cgrp-like immunoreactivity was localized to axons innervating the rostral and dorsal vta. direct administration of cgrp to the vta of the rat resulted in a dose-related increase in da utilization in the medial prefrontal cortex, but not other mesocortical, mesolimbic, or striatal da terminal field r ...	1987	3554009
[use of ganglioside-containing magnetic polyacrylamide sorbents for the immunoenzyme method of determining cholera enterotoxin].	a variant of eia techniques for the determination of cholera enterotoxin is proposed. this method is based on the selective sorption of the toxin on ganglioside-containing magnetic granules with its subsequent detection by means of immune serum and antispecific immunoperoxidase conjugate. the proposed method permits the detection of 0.052 +/- 0.02 ng of protein of the purified toxin.	1987	3554849
correlation between anorectic potency and affinity for hypothalamic (+)-amphetamine binding sites of phenylethylamines.	the potencies of a number of phenylethylamines and related compounds to inhibit food intake were compared with their relative affinities for hypothalamic (+)-amphetamine binding sites in vitro. there was a positive correlation (r = 0.78, p less than 0.001, n = 19) between potencies of these compounds to inhibit food intake and their potencies to inhibit [3h](+)-amphetamine binding. individual enantiomers of amphetamine, ephedrine, norephedrine, pseudoephedrine and norpseudoephedrine all inhibite ...	1987	3556402
cell-associated hemagglutinin-deficient mutant of vibrio cholerae.	cell-associated hemagglutinin-negative mutants were derived from cholera enterotoxin-negative vibrio cholerae jbk70 by tn5 mutagenesis. one of the mutants identified, sb001, was characterized in greater detail. its ability to colonize ilea of adult rabbits was determined by feeding approximately 10(8) v. cholerae to each animal. at 17 h after feeding, the numbers of viable vibrios in the ilea were determined. there was a significant, 4 log, decrease in the ability of the hemagglutinin-negative m ...	1987	3557620
voluntary hyperventilation changes recruitment order of parasternal intercostal motor units.	the order of recruitment of single-motor units in parasternal intercostal muscles during inspiration was studied in normal human subjects during quiet breathing and voluntary hyperventilation. electromyograms were recorded from the second and third intercostal spaces by means of bipolar fine wire electrodes. flow at the mouth, volume, end-expired co2, and rib cage and abdominal anterior-posterior diameters were monitored. single-motor units were identified using criteria of amplitude and shape, ...	1987	3558179
effect of autonomic blockade on tracheobronchial blood flow.	tracheobronchial blood flow increases two to five times in response to cold and warm dry air hyperventilation in anesthetized tracheostomized dogs. in this series of experiments we have attempted to attenuate this increase by blockade of the autonomic nervous system. four groups of anesthetized, tracheostomized, open-chest dogs were studied. group 1 (n = 5) were hyperventilated for 30 min with 1) warm humid [approximately 26 degrees c, 100% relative humidity, (rh)] air followed by bilateral vago ...	1987	3558211
n-butyrate increases the level of thyroid hormone nuclear receptor in non-pituitary cultured cells.	the thyroid hormone nuclear receptor is a chromatin-associated protein regulating expression of specific genes. acetylation of nucleosomal core histones is thought to be one of the factors regulating transcriptional activity of chromatin, and it is suggested that this reaction negatively regulates thyroid hormone receptor levels in gh1 cells (samuels, h.h., stanley, f., casanova, j., and shao, t. c. j. biol. chem. 255, 2499-2508). in the present study, we found that n-butyrate, a potent inhibito ...	1987	3558403
strain energy density function and uniform strain hypothesis for arterial mechanics.	stress distribution through the wall thickness of the canine carotid artery was analyzed on the basis of the uniform strain hypothesis in which the wall circumferential strain was assumed to be constant over the wall cross-section under physiological loading condition. a newly proposed logarithmic type of strain energy density function was used to describe the wall properties. in contrast with other studies, this hypothesis gave almost uniform distribution of wall stresses under the physiologica ...	1987	3558431
straight intracisternal tubules in rough endoplasmic reticulum of human melanoma cells.	this case presentation illustrates peculiar tubular aggregates in rough endoplasmic reticulum (rer) of human melanoma cells, a location in which they have been reported in a small percentage of malignant melanomas.	1987	3686713
rev 5901 and ly 171,883 protect rat gastric mucosa against ethanol-induced damage.	the effect of the leukotriene antagonist, ly 171,883, or the 5-lipoxygenase inhibitor, rev 5901, on ethanol-induced gastric lesion formation in the rat was investigated. pretreatment with rev 5901 resulted in a dose dependent decrease in lesion length. doses of 32 and 64 mg/kg induced nearly complete protection against ethanol, while doses of 1 and 8 mg/kg were much less effective. with ly 171,883, 32 and 64 mg/kg doses less dramatically reduced lesion length. these findings implicate products o ...	1987	3687588
distribution of immunoreactive inhibin like material in different regions of human prostate.	distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05).	1987	3688474
cholera after the consumption of raw oysters. a case report.	in august 1986, a 76-year-old woman in miami, florida, developed profuse watery diarrhea and abdominal cramps. two and four days before the onset of her illness, she had eaten six raw oysters at each of two restaurants in miami. a stool specimen yielded toxigenic vibrio cholerae o1 biotype el tor, serotype inaba. the results of toxin gene probing of the organism recovered from the patient differed significantly from those of other v. cholerae o1 isolates from the gulf coast and elsewhere in the ...	1987	3688678
vitamin e protects proteins against free radical damage in lipid environments.	the fragmentation of the membrane protein monoamine oxidase in submitochondrial particles was induced by defined free radicals during radiolysis and by a system dependent on hydrogen peroxide and a transition metal. by injection of alpha-tocopherol in vivo, the levels of this physiological antioxidant in the mitochondrial preparations could be elevated more than ten-fold. in both radical-generating systems the presence of high levels of alpha-tocopherol in the membrane substantially retarded the ...	1987	3689396
fluorescence immunohistochemical detection of hypoxic cells in spheroids and tumours.	polyclonal antibodies have been raised in rabbits to a haemocyanin adduct of a reductively-activated, fluorinated analogue of misonidazole. fluorescence immunohistochemical studies show that the polyclonal antibodies bind to spheroid sections and tumour sections in patterns similar to those revealed by autoradiographic studies with a tritium-labelled derivative of the fluorinated misonidazole analogue.	1987	3689657
prolonged exposure to m. faeni in strain ii guinea-pigs: pulmonary interstitial inflammation.	models of hypersensitivity pneumonitis (hp) should exhibit progression of pulmonary histological abnormalities during continuing challenges. strain ii guinea-pigs were sensitized with micropolyspora faeni and received 2, 4, or 8 weekly intratracheal (i.t.) particulate m. faeni challenges. control animals received normal saline (ns). four days after the last exposure, randomly selected microscopic fields of lung (200/animal) were judged to be normal or abnormal. if abnormal, the location and natu ...	1987	3689675
[change in tissue glycogen reserves in tumor bearers as a reflection of a hypoglycemic stress syndrome].	glycogen content in the brain, liver and skeletal muscles of rats bearing ascite zajdela hepatoma (azh) and solid 27 hepatoma (27-h) has been studied. serum glucose levels directly correlated with liver glycogen reserves. in the terminal stage of tumor growth depletion of liver glycogen was observed, while the stores of muscle glycogen did not diminish. within 1-4 days (azh) and 15-30 days (27-h) after implantation the stores even exceeded those of control healthy rats. in the terminal stage, in ...	1987	3689968
age-dependent activation of glucocorticoid receptors in the cerebral hemispheres of male rats.	the binding of [3h]dexamethasone-receptor complexes to purified nuclei was studied in the cerebral hemispheres of immature (3-week-old) and mature (26-week-old) long-evans male rats to determine the age-related changes, if any, in the physicochemical properties of glucocorticoid receptors. our data show that heat activation (for 45 min at 25 degrees c) significantly enhances the nuclear binding of [3h]dexamethasone-receptor complexes in rats of both ages, with a greater magnitude in immature rat ...	1987	3690338
[effects of adrenaline and cold stimulation on erythrocyte membranes in the rat].		1987	3690760
[an ecological study of vibrio cholerae non o-1, in comparison with vibrio cholerae o-1 in regard to the circumstance of water].		1987	3693523
rapid diagnosis of cholera by coagglutination test using 4-h fecal enrichment cultures.	a simple, rapid, and reliable method to detect vibrio cholerae in fecal specimens would assist in the management of cases of severe diarrhea, especially since most such cases occur in areas with minimal laboratory facilities. a coagglutination test was used to detect v. cholerae antigen in bile-peptone broth incubated with feces. in the technique, staphylococcus aureus cowan 1 coated with anti-v. cholerae o1 antiserum was tested with cultures incubated for 4 h. when 165 specimens were tested, th ...	1987	3693549
altered metabolic enzyme activities in fast and slow twitch muscles due to induced sciatic neuropathy in the rat.	a pilot study was conducted to examine enzymatic and metabolic alterations in end organs as a consequence of neuropathy. silastic pellets were implanted transverse to the sciatic nerve of rats. neurobehavioral evaluations based on hind limb gait were conducted at 2 and 4 weeks postoperatively. four-week values demonstrated facilitated utilization of the neuropathic limb in three of five animals, as compared to the normal contralateral limb and normal animals. nerve electrophysiology and quantita ...	1987	3694060
transmission of pasteurella multocida in rabbits.	contact and airborne transmission of p. multocida in rabbits was evaluated in an artificially controlled environment. transmission by contact occurred more readily from rabbits with acute infections than from rabbits with chronic infections. however, airborne transmission to rabbits in adjacent cages did not occur.	1987	3695397
involvement of sialic acid in high-affinity uptake of dopamine by synaptosomes from rat brain.	the high-affinity, sodium-dependent uptake of dopamine (da) was inhibited by the pretreatment of synaptosomes with neuraminidase from vibrio cholerae. the inhibition was of a non-competitive type, resulting in a 40% decrease of vmax. neither basal nor depolarization-stimulated release of da was affected. treatment of synaptosomes with neuraminidase caused a 48% loss of sialic acid from the lipid-bound pool and a 80% decrease in the protein-bound fraction. the inhibition of da uptake was found to ...	1987	3696480
population biology of ostertagia ostertagi and anthelmintic strategies against ostertagiasis in calves.	five chemoprophylactic or chemotherapeutic strategies against bovine ostertagiasis are compared using a mathematical model of the population biology of ostertagia ostertagi. the model offers a means of screening novel strategies prior to their further investigation in the field. under conditions of climate and management typical of many regions in northern temperate europe, the model indicates that all of the tested prophylactic strategies will result in a profound reduction in the intensity of ...	1987	3696773
[effects of leanness and emaciation of cattle on quantitative characteristics of meat].	the studies were carried out on 60 cows of the lowland black-and-white breed at about 4 years of age, which were divided into 4 groups according to the degree of fatness: well-fed, lean, moderately and completely emaciated. the basic composition, water holding capacity, miofibrillar protein, collagen, amino acid composition of proteins, biological value of muscle proteins, dna level, ph, physical parameters of muscle fibres and sensory properties of meat were determined. it was found that: a) wi ...	1987	3697339
therapeutic effect of dexamethasone in t-2 toxicosis.	t-2 toxin is a mycotoxin that induces toxemia characterized by numerous hematological and biochemical changes. we have previously shown that prostaglandin (pg) production in brain tissue is increased following t-2 toxin. the present study was designed in order to test the effect of dexamethasone on brain prostaglandins and survival of rats subjected to t-2 toxin. furthermore, the effect of bw 755c, a dual inhibitor of the cyclooxygenase and lipoxygenase pathways of arachidonate metabolism, on th ...	1987	3150046
purification of an octamer sequence (atgcaaat)-binding protein from human b cells.	the highly conserved octamer sequence atgcaaat or its inverse complement found in all human and murine immunoglobulin gene promoters has been demonstrated to be necessary in the lymphoid-specific transcription by deletion analysis. trans-acting factors that interact with the octamer motif are thought to be involved in this tissue-specific expression. using a gel mobility shift assay, we have identified both lymphoid-specific and ubiquitous nuclear factors that interact with a human gamma 1 heavy ...	1987	3122182
mucosal and serum immunity following commensal enteric colonization.		1987	3122537
reflex regulation of atrioventricular conduction.	we evaluated the time course of baroreflex modulation of atrioventricular (av) nodal conduction in anesthetized dogs (n = 28). beat-by-beat changes in heart rate (hr) and av interval (avi) evoked by transient alterations in arterial pressure (ap) were recorded in the intact state, after vagotomy, and following stellectomy. under each experimental condition, alterations in ap induced parallel changes in hr and avi with maximum hr and avi responses occurring simultaneously. in three animals, ap al ...	1987	3109256
drug-resistant tuberculosis in a southern california hospital. trends from 1969 to 1984.	resistance to one or more antituberculosis drugs was found in 98 of 281 (35%) patients hospitalized at harbor-ucla with culture-positive tuberculosis between 1980 and 1984. resistance to antituberculosis drugs occurred in 23% of patients who had not been previously treated, whereas previously treated patients had a 59% rate of resistance. the overall rates of drug resistance had not significantly changed from prior studies at this hospital. drug resistance was also found in patients with extrapu ...	1987	3109291
changing pattern of bio and serotypes of vibrio cholerae 01 in rural bangladesh.		1987	3106666
characterization of self-transmissible plasmids determining lactose fermentation and multiple antibiotic resistance in clinical strains of klebsiella pneumoniae.	the lactose fermentation (lac+) and antibiotic resistance (r+) phenotypes were conjugally transferred from klebsiella pneumoniae strains (k166, k182, k186, k218, and k220) to salmonella typhi, s. typhimurium, shigella flexneri, and vibrio cholerae. the genes for lactose fermentation and antibiotic resistance were located on the plasmids. further analysis of plasmid dna from these isolates indicated the presence of multiple plasmids (mr ranged less than 2.7 to 70 x 10(6)). the lac+r+ plasmids p16 ...	1987	3107002
endothelial cell proliferation may be mediated via the production of endogenous lipoxygenase metabolites.	endogenous regulators of endothelial cell proliferation have not been clearly defined. we investigated whether the cyclooxygenase and/or lipoxygenase metabolites are involved in this process, and report that lipoxygenase products can modulate endothelial cell growth. nordihydroguaiaretic acid--a lipoxygenase inhibitor, inhibited endothelial cell proliferation as well as dna synthesis. 5,8,11,14-eicosatetraynoic acid--an inhibitor of both lipoxygenase and cyclooxygenase also inhibited endothelial ...	1987	3107553
biosynthesis and processing of platelet gpiib-iiia in human megakaryocytes.	platelet membrane glycoprotein iib-iiia forms a calcium-dependent heterodimer and constitutes the fibrinogen receptor on stimulated platelets. gpiib is a two-chain protein containing disulfide-linked alpha and beta subunits. gpiiia is a single chain protein. these proteins are synthesized in the bone marrow by megakaryocytes, but the study of their synthesis has been hampered by the difficulty in obtaining enriched population of megakaryocytes in large numbers. to examine the biosynthesis and pr ...	1987	3108266
comparison of the chinese schema and the international antigenic typing system for serotyping pseudomonas aeruginosa.	twelve strains of pseudomonas aeruginosa representing 12 serogroups in the serogrouping schema used in the people's republic of china were compared with serogroups in the international antigenic typing system (iats). the first eight groups originated in the people's republic of china, and group ii appears to have a new major antigen that is not found in the iats. groups i, iii, iv, v, vi, vii, and viii correspond to groups 11, 6, 9, 4, 8, 3, and 1, respectively, of the iats. groups ix, x, xi, an ...	1987	3108310