Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| the effects of a commercial beverage on the enamel surface of human tooth: a scanning electron microscopy study. | 1987 | 3436273 | |
| naturally occurring car bacillus infection in a laboratory rat colony and epizootiological observations. | an epizootic of chronic respiratory disease was found in a rat colony. lungs of the symptomatic rats showed histopathologically severe peribronchial lymphoid cuffing. filamentous bacteria were detected on the border of the tracheal and bronchial epithelium by light and electron microscopy. these bacteria did not grown on artificial media but propagated in embryonated chicken eggs. the disease was thus diagnosed as cilia-associated respiratory (car) bacillus infection. epizootiological observatio ... | 1987 | 3436371 |
| re: prophylaxis of gram-negative and gram-positive infections in rodents with three intravenous immunoglobulins and therapy of experimental polymicrobial burn wound sepsis with pseudomonas immunoglobulin and ciprofloxacin. | 1987 | 3436681 | |
| in vitro activity and mode of action of fluoroquinolones. | the in vitro activity of nine fluoroquinolones has been determined by agar dilution method against 1100 clinical isolates of different microorganisms: 412 enterobacteriaceae, 93 non-fermenters, 398 enteropathogens, 140 gram-positive cocci, 31 haemophilus and 26 gonococci. the bactericidal activity tested in broth against different representative isolates was generally similar to the inhibitory concentration. killing kinetics studied in different isolates of enterobacteriaceae, in pseudomonas aer ... | 1987 | 3438148 |
| [construction of the v. cholerae toxin gene probe and its use for the detection of v. cholerae]. | 1987 | 3439159 | |
| [ultrastructural research on the kidneys of rats treated with organophosphorus pesticides and infected with erysipelothrix rhusiopathiae]. | 1987 | 3439487 | |
| [involvement of the growth hormone in rapid increases of the potassium concentration of the serum]. | as a rule, the infusion of arginine hydrochloride is followed by an increase in serum potassium. the investigation is concerned with the short-term effect of growth hormone, which can be stimulated by arginine (0.6 g/kg body weight), on potassium concentrations in rats and patients with cerebral death. the administration of human somatotropin causes a significant rise in potassium concentrations in patients with cerebral death (0.4 iu/kg body weight) and in rats (4.0 iu/kg body weight). in compa ... | 1987 | 3439594 |
| tumor necrosis factor is partly responsible for the differentiation of mouse myeloid leukemia cells. | when mouse myeloid leukemia m1 cells were incubated with 0.1% tns(serum containing tumor necrosis factor, tnf), they were induced to differentiate into macrophage-like cells within 14 h. the stability of the differentiation activity to heat treatment was very similar to that of the cytotoxicity of tnf for l929 cells. the differentiation activity could not be separated from the tnf activity by sequential chromatography procedures using deae-sephadex and sephacryl s-200 or by isoelectric focusing. ... | 1987 | 3440028 |
| two-hour miniaturized system for detection of enteropathogenic bacteria in stool cultures. | a miniaturized 2-h system for detection of lactose-negative potentially enteropathogenic bacteria on the basis of nine enzymatic tests was evaluated. the system was challenged with 210 strains of lactose-negative and lactose-positive species grown on macconkey agar. specific and constant patterns were found for salmonella, shigella a-c, yersinia enterocolitica, aeromonas spp. and vibrio cholerae. shigella sonnei and hafnia alvei had an identical pattern, likewise plesiomonas shigelloides, haloph ... | 1987 | 3440458 |
| clinical features of covert toxocariasis. | the clinical features found in 14 children with toxocara elisa titres of greater than or equal to 0.7 were compared with those found in 34 toxocara negative children. blood eosinophils were elevated in 7 of the 14 patients and the highest eosinophilia was 14%. there was a highly significant association between hepatomegaly, cough, sleep disturbance and a raised titre (p less than 0.01). behaviour disturbance, abdominal pain and headache were also significantly associated with a raised titre (p l ... | 1987 | 3441751 |
| destructive chronic borrelia meningoencephalitis in a child untreated for 15 years. | a case of borrelia spirochetosis with recurring episodes of progressively destructive chronic meningoencephalitis, untreated for 15 years, is presented. the patient, now 23 years old, represents an example of the natural course of the disease and may serve as a reminder of the diagnosis in retrospective cases as well as prospectively for acute and chronic states of serous meningitis/meningoencephalitis both in children and adults. | 1987 | 3441752 |
| retardation of cell cycle progression in yeast cells recovering from dna damage: a study at the single cell level. | pedigree analyses of individual yeast cells recovering from dna damage were performed and time intervals between morphological landmark events during the cell cycle (bud emergence and cell separation), were recorded for three generations. the associated nuclear behavior was monitored with the aid of dapi staining. the following observations were made: all agents tested (x-rays, mms, ems, mnng, nitrous acid) delayed the first bud emergence after treatment, which indicates inhibition of the initia ... | 1987 | 3302599 |
| the aetiology of diarrhoea in children at an urban hospital in bangladesh. | enteric pathogens were studied in 104 cases with acute diarrhoea and in 74 age and sex matched concurrent controls. one or more pathogens were isolated from 59.1% of cases compared with 20.4% of controls (p less than 0.001). single enteropathogens were detected in 33.7% and multiple enteropathogens in 25.4% of the cases. enteropathogenic escherichia coli (epec), campylobacter jejuni, rotavirus, vibrio cholerae non-o1 and enterotoxigenic escherichia coli were the major pathogens detected. the hig ... | 1987 | 3303485 |
| [genetic determination of the vibriocinogenicity trait in vibrio cholerae of the el tor biotype]. | in two different strains of cholera vibrios two reca-dependent plasmids, pvib i (1.9-2.2 md) and pvib ii (5.2-5.8 md), have been detected. these plasmids determine the synthesis of vibriocin, coagulase and fibrinolysin, which has been established by the cotransformation of the dna of plasmids pvib i and pbr322 and by the transfer mobilization with the use of plasmid rp4. | 1987 | 3303760 |
| sialyltransferases as specific cell surface probes of terminal and penultimate saccharide structures on living cells. | rat liver beta-galactoside alpha-2,6-sialyltransferase and vibrio cholerae sialidase were used, in conjunction with cmp-n-acetyl-[3h]neuraminic acid, to probe the glycoconjugate distribution, sialylation state, and level of penultimate gal beta 1-4glcnac residues on the surfaces of murine thymic lymphocytes. we report a detailed characterization of this sialyltransferase-mediated labeling system. exogenous sialylation of intact cells is dependent on transferase, sugar nucleotide donor, cell numb ... | 1987 | 3304006 |
| different levels of dna methylation in yeast and mycelial forms of candida albicans. | isotope dilution gas chromatography-mass spectrometry analysis of genomic dnas isolated from three candida albicans isolates showed significant differences in the amounts of 5-methyldeoxycytidine (m5cyt) in dna from yeast-form and from mycelial-form cells; the moles percent m5cyt were 0.11, 0.11, and 0.097 for yeast-form dna and 0.045, 0.053, and 0.047 for mycelial-form dna for the three isolates sh8, 9938, and b311, respectively. the lower m5cyt values for mycelial-form cells suggest that those ... | 1987 | 3305486 |
| [use of a new method for restoring the type properties of atypical vibrio cholerae]. | subcultures with a number of signs characteristic of epidemically significant strains have been isolated from cholera vibrios, nonpathogenic and atypical in a number of properties, by a new in vitro method developed by the authors. this method makes it possible to increase the virulence of poorly agglutinating cultures of v. cholerae o1 and their agglutinability with cholera antisera. | 1987 | 3307237 |
| the k1 beta-lactamase of klebsiella pneumoniae. | beta-lactamase k1 was purified from klebsiella pneumoniae sc10436. it is very similar to the enzyme produced by klebsiella aerogenes 1082e and described by emanuel, gagnon & waley [biochem. j. (1986) 234, 343-347]. an active-site peptide was isolated after labelling of the enzyme with tritiated beta-iodopenicillanate. a cysteine residue was found just before the active-site serine residue. this result could explain the properties of the enzyme after modification by thiol-blocking reagents. the s ... | 1987 | 3307765 |
| manipulation of t-cell content in transplanted human bone marrow: effect on gvh and gvl reactions. | 1987 | 3310003 | |
| actin localization in male germ cell intercellular bridges in the rat and ground squirrel and disruption of bridges by cytochalasin d. | filaments about 6-7 nm in diameter were seen associated with germ cell intercellular bridges in detergent-permeabilized cells treated with tannic acid. approximately 40-50 filaments were present subjacent to the bridge density. filaments encircled the bridge channel in a manner similar to contractile ring actin filaments of dividing cells. nbd-phallacidin and myosin s-1 subfragments were employed to demonstrate that the filaments observed at intercellular bridges are actin. intratesticular injec ... | 1987 | 3310595 |
| morphometry and cytochemistry of leydig cells in experimental diabetes. | leydig cell ultrastructure and function in diabetic rats were studied by concurrent cytochemistry, morphometry, and testosterone assay. the streptozotocin (stz) model was modified to include nondiabetic stz-injected rats, an insulin-treated diabetic group, and semistarved animals in addition to controls and untreated diabetic rats. the separation of the effects of diabetes, stz, semistarvation, and insulin treatments was achieved by application of orthogonal contrast statistics. after 3 months o ... | 1987 | 3310596 |
| methods for the assessment of human body composition: traditional and new. | renewed interest in the assessment of human body composition has stimulated the need for a balanced understanding of available methodologies of estimating fat-free mass and percent body fat. this review summarizes the physical bases and assumptions, describes applications, and discusses the theoretical and practical limitations of currently available indirect methods. although standard methods are discussed, recent modifications and adaptations are emphasized. | 1987 | 3310598 |
| koch's comma bacillus: the first year. | 1987 | 3311246 | |
| single dose tetracycline in cholera. | a randomised clinical trial was carried out to explore the efficacy of single dose tetracycline therapy in cholera. one hundred and eighteen adult patients were assigned to receive either tetracycline in a single 1 g, or a single 2 g dose, or tetracycline 500 mg every six hours four times, or no antibiotics as controls. the means of total liquid stool volumes after treatment were lower in the single 1 g dose group (168.0 +/- 20.9 ml/kg), in single 2 g dose group (229.5 +/- 45.6 ml/kg), and multi ... | 1987 | 3311904 |
| estrogen-induced lysosomal proteases secreted by breast cancer cells: a role in carcinogenesis? | in an attempt to understand the mechanism by which estrogens stimulate cell proliferation and mammary carcinogenesis, metastatic human breast cancer cell lines (mcf7, zr75-1) were found to secrete a 52,000 dalton (52k) protein under estrogen stimulation. following its purification to homogeneity, the 52k protein was identified as a secreted procathepsin-d-like aspartyl protease bearing mannose-6-phosphate signals. this precursor displays an in vitro autocrine mitogenic activity on estrogen-depri ... | 1987 | 3312245 |
| estrogen-induced lysosomal proteases secreted by breast cancer cells: a role in carcinogenesis? | in an attempt to understand the mechanism by which estrogens stimulate cell proliferation and mammary carcinogenesis, metastatic human breast cancer cell lines (mcf7, zr75-1) were found to secrete a 52,000 dalton (52k) protein under estrogen stimulation. following its purification to homogeneity, the 52k protein was identified as a secreted procathepsin-d-like aspartyl protease bearing mannose-6-phosphate signals. this precursor displays an in vitro autocrine mitogenic activity on estrogen-depri ... | 1987 | 3312245 |
| intraosseous infusions. | 1987 | 3312550 | |
| [confirmative analysis for digitoxose in the alkali extract of candida albicans cells]. | 1987 | 3312706 | |
| morphometric investigation of the microvascular system of pancreatic exocrine and endocrine tissue in the rat. | morphometric data were obtained from the microvasculature in exocrine and endocrine tissue of the rat pancreas. two groups of anesthetized rats (100 and 300 g body wt, respectively), received intravenous injections of fluorochrome-coupled globulins in order to label the entire vascular system. after circulation of fluorescein-isothiocyanate (fitc)-globulin through the vascular system for 30 min and lissamine rhodamine b 200 (rb 200)-globulin for 5 min, the pancreas was frozen rapidly. in frozen ... | 1987 | 3312966 |
| oral vaccine against cholera prepared from vibrio cholerae antigen(s). | albino rats aged 7-8 weeks old purchased from the national laboratory animal centre, salaya, nakhon pathom, were found to be a good animal model for the study on immunogenicity of v. cholerae antigens. seventy-two rats were fasted for 15 hours before feeding each one with 1 ml of 5% nahco3 to reduce gastric acidity prior to immunization. they were divided into 9 groups of 8 rats and immunized orally with 2 ml, each, of the v. cholerae antigens dissolved or suspended in cassamino acid as follows: ... | 1987 | 3313733 |
| [basic factors in the pathogenicity of vibrio cholerae and their genetic control]. | 1987 | 3314272 | |
| antimicrobial activity of tertiary amine covalently bonded to a polystyrene fiber. | tertiary amine was covalently bonded to a polystyrene fiber and examined for antibacterial activity. the tertiary amine covalently bonded to a polystyrene fiber (taf) showed a high antimicrobial activity against escherichia coli. taf exhibited a stronger antibacterial activity against gram-negative bacteria (e. coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhimurium, and serratia marcescens) than against gram-positive bacteria (staphylococcus aureus and streptococcus faecalis ... | 1987 | 3314703 |
| measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study. | a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ... | 1987 | 3315296 |
| measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study. | a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ... | 1987 | 3315296 |
| tissue culture of human fetal pancreas. effects of human serum on development and endocrine function of isletlike cell clusters. | the human fetal pancreas represents a source of insulin-producing beta-cells with a potential for transplantation to diabetic patients. it has previously been shown that such cells can be viably maintained in tissue culture media containing fetal calf serum (fcs) and that these explants continue to synthesize and release insulin. in this study the effects of human serum (hs) on the growth and function of human fetal pancreatic explants have been compared with those of fcs. for this purpose, panc ... | 1987 | 3315788 |
| abo blood groups and escherichia coli sepsis. | 1987 | 3318030 | |
| extracellular matrix of the human lamina cribrosa. | we used immunoperoxidase staining and double immunofluorescent staining to demonstrate the macromolecular components of the extracellular matrix of the lamina cribrosa from young human donors. the cribriform plates were made up of a core of elastin fibers with a sparse, patchy distribution of collagen type iii. the plates were coated with collagen type iv and laminin; these basement membrane components were presumably made by the astrocytes that were distributed on the surfaces of the plates. th ... | 1987 | 3318474 |
| [medico-genetic studies of human provisional organs]. | 1987 | 3318529 | |
| omental pedicle graft in the management of infected ascending aortic prostheses. | two patients had mediastinal infections with chronic draining sinus tracts that involved a vascular prosthesis in the ascending aorta. in 1 patient, a false mycotic aneurysm developed and in the other, a beginning rupture of the proximal suture line. in both patients, the infection was cured by replacing the infected aortic prosthesis combined with wrapping the new prosthesis with a pedicled omental graft. an omental graft was used to protect the vascular prosthesis and minimize the risk of recu ... | 1987 | 3318738 |
| effect of gdp on the interactions between chloroplast ef-ts and chloroplast and e. coli ef-tu. | the effects of varying concentrations of gdp on the stability of homologous and heterologous ef-tu:ef-ts complexes formed with the elongation factors from the chloroplast of euglena gracilis and from e. coli have been investigated. the complexes formed with chloroplast ef-ts were significantly more stable to gdp-induced dissociation than those formed with e. coli ef-ts. the complex between chloroplast ef-tu and chloroplast ef-ts required nearly 1,000-fold higher concentrations of gdp for dissoci ... | 1987 | 3318834 |
| localization and retention of mycobacterial antigen in lymph nodes of leprosy patients. | although leprosy, a chronic disease caused by m. leprae, primarily affects skin and peripheral nerves, pathological changes and granulomas have been observed in lymph nodes which are: (a) present in tuberculoid lymph nodes in the absence of acid-fact bacilli and (b) persistent in lepromatous patients even after prolonged treatment. we detected substantial amounts of mycobacterial antigen in 16 leprous lymph nodes using anti-bcg by the peroxidase anti-peroxidase method. the load and distribution ... | 1987 | 3318906 |
| localization and retention of mycobacterial antigen in lymph nodes of leprosy patients. | although leprosy, a chronic disease caused by m. leprae, primarily affects skin and peripheral nerves, pathological changes and granulomas have been observed in lymph nodes which are: (a) present in tuberculoid lymph nodes in the absence of acid-fact bacilli and (b) persistent in lepromatous patients even after prolonged treatment. we detected substantial amounts of mycobacterial antigen in 16 leprous lymph nodes using anti-bcg by the peroxidase anti-peroxidase method. the load and distribution ... | 1987 | 3318906 |
| improved immunohistochemical staining of angiotensin ii in rat brain using affinity purified antibodies. | recent immunohistochemical studies that have sought to detect angiotensin ii/iii (aii/aiii) immunoreactive material in the brain have been forced to rely on a small number of antisera because most aii/aiii antibodies have unexplainably proved unsuitable for immunohistochemistry. although extremely useful tools, these antisera have suffered from high background staining. the purpose of this study was to re-examine and characterize the staining using the most popular aii/aiii antiserum (denise) be ... | 1987 | 3319044 |
| formamidopyrimidine-dna glycosylase of escherichia coli: cloning and sequencing of the fpg structural gene and overproduction of the protein. | an escherichia coli genomic library composed of large dna fragments (10-15 kb) was constructed using the plasmid pbr322 as vector. from it 700 clones were individually screened for increased excision of the ring-opened form of n7-methylguanine (2-6-diamino-4-hydroxy-5n-methyl-formamidopyrimidine) or fapy. one clone overproduced the fapy-dna glycosylase activity by a factor of 10-fold as compared with the wild-type strain. the fapy-dna glycosylase overproducer character was associated with a 15-k ... | 1987 | 3319582 |
| [localization of structural genes of vibrio cholerae cholerae toxin using the recombinant plasmid rp4omega elt]. | the recombinant plasmid rp4 omega elt carrying escherichia coli heat-labile enterotoxin elt genes with 70-80% homology with genes vct of vibrio cholerae has been constructed. we used this plasmid to determine localization of the cholerae toxin genes vct on the map of vibrio cholerae cholerae. two types of the donors were revealed in matings of 10 strains of v. cholerae cholerae 569b/rp4 omega elt with the polyauxotrophic recipients rv31 and rv175: some strains had enhanced frequency of mobilizat ... | 1987 | 3319775 |
| evidence that dna involved in the expression of nodulation (nod) genes in rhizobium binds to the product of the regulatory gene nodd. | in rhizobium leguminosarum biovar viciae, the regulatory nodulation nodd gene has at least two functions. it constitutively represses its own transcription and in the presence of inducer flavonoid molecules, it activates the expression of two other nod gene transcriptional units, nodabcij and nodfe. upstream of noda and nodf is a conserved sequence, the nod box, which has been implicated in nodd-mediated transcriptional activation of these genes. dna fragments spanning the nod boxes that precede ... | 1987 | 3320955 |
| mode of action of nitro-heterocyclic compounds on escherichia coli. | the inhibitory and bactericidal activities of several different nitro-heterocyclic compounds, such as nitrofuran, nitronaphthofuran, nitrobenzofuran, nitroimidazole and nitrothiazole, were assessed in vitro. all these substances except nitroimidazole were active against escherichia coli, though to different degrees. under anaerobic test conditions the antibacterial activity increased slightly. nitroreductase-deficient mutants, however, were highly resistant to all nitro-compounds, indicating tha ... | 1987 | 3322751 |
| long-term in vitro cultivation of plasmodium berghei. | 1987 | 3323090 | |
| sterol and phospholipid acyl chain alterations in saccharomyces cerevisiae secretion mutants as a function of temperature stress. | analyses of free sterol, steryl ester and fatty acid components from yeast secretion mutants indicated that free and esterified sterol remained relatively constant over a growth range of 24 c to 34 c. the saturated fatty acid components (16:0 and 18:0) increased while the unsaturated fatty acids (16:1 and 18:1) decreased as the growth temperature increased. in secretory mutants, fatty acid composition changes are more pronounced than in the wild-type strain. a shift toward increased saturated an ... | 1987 | 3323755 |
| sterol and phospholipid acyl chain alterations in saccharomyces cerevisiae secretion mutants as a function of temperature stress. | analyses of free sterol, steryl ester and fatty acid components from yeast secretion mutants indicated that free and esterified sterol remained relatively constant over a growth range of 24 c to 34 c. the saturated fatty acid components (16:0 and 18:0) increased while the unsaturated fatty acids (16:1 and 18:1) decreased as the growth temperature increased. in secretory mutants, fatty acid composition changes are more pronounced than in the wild-type strain. a shift toward increased saturated an ... | 1987 | 3323755 |
| physiological role of growth hormone in adult life. | some historical background on the physiological effects of pituitary gland extract and later of purified hgh in experimental animals is given, and the regulatory role of hgh in the human adult is reviewed. the effects of hgh on carbohydrate, protein and lipid metabolism are complex and closely linked to the action of other hormones, particularly insulin. | 1987 | 3324637 |
| enumeration of vibrio cholerae o1 in bangladesh waters by fluorescent-antibody direct viable count. | a field trial to enumerate vibrio cholerae o1 in aquatic environments in bangladesh was conducted, comparing fluorescent-antibody direct viable count with culture detection by the most-probable-number index. specificity of a monoclonal antibody prepared against the o1 antigen was assessed and incorporated into the fluorescence staining method. all pond and water samples yielded higher counts of viable v. cholerae o1 by fluorescent-antibody direct viable count than by the most-probable-number ind ... | 1987 | 3324967 |
| a new keratinolytic proteinase from clinical isolates of trichophyton mentagrophytes. | 1987 | 3325542 | |
| ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis. | ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ... | 1987 | 3326399 |
| ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis. | ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ... | 1987 | 3326399 |
| indirect immunofluorescence application in the epidemiological study of hepatitis a. | we used iif test for seroepidemiological study on 531 sera divided in age-groups obtained from apparently healthy subjects of rome. our results confirm the endemicity of hav (hepatitis a virus) infection in rome with a prevalence of more than 50%. the application of the anti-hav iif (indirect immunofluorescence) test to a large number of sera demonstrated its usefulness as a quantitative and qualitative test in the epidemiological field. | 1987 | 3327512 |
| liver membrane antibodies in chronic delta hepatitis. | the correlation between the presence of liver membrane antibodies (lma) and delta superinfection in chronic hepatitis b was studied in 136 chronic hepatitis patients, 20 hepatitis b surface antigen asymptomatic carriers and 50 healthy controls, by testing for the presence of lma, hepatitis b virus markers and anti-delta (anti-hd). neither anti-hd nor lma were detected in controls or in asymptomatic carriers. lma positivity was statistically higher (p less than 0.001) in anti-hd-positive patients ... | 1987 | 3327717 |
| control of tick-borne diseases. | 1987 | 3294675 | |
| valanimycin acts on dna in bacterial cells. | 1987 | 3294774 | |
| effects of estradiol-17 beta, naloxone and gonadotropin releasing hormone on postpartum secretion of luteinizing hormone in fall-lambing ewes. | the interaction among exogenous estradiol-17 beta, naloxone and gonadotropin releasing hormone (gnrh) in the control of luteinizing hormone (lh) secretion was studied in intact postpartum ewes nursing their offspring. one-half of 30 fall-lambing ewes were implanted subcutaneously with an estradiol-17 beta containing silastic capsule between postpartum d 1 and 12 which doubled their serum concentrations of estradiol (16.0 +/- .1 vs 8.4 +/- .1 pg/ml). blood samples were collected from implanted an ... | 1987 | 3294777 |
| an aminoacyl-trna synthetase complex in escherichia coli. | aminoacyl-trna synthetases from several strains of escherichia coli are shown to elute as a high-molecular-weight complex on 6% agarose columns (bio-gel a-5m). in contrast, very little synthetase activity was observed in such complexes on sephadex g-200 columns, suggesting that these enzymes may interact with or are dissociated during chromatography on dextran. the size of the complex observed on bio-gel a-5m was influenced by the method of cell breakage and the salt concentrations present in bu ... | 1987 | 3294804 |
| [studies on changes in the uterine cervix during pregnancy]. | the uterine cervix becomes increasingly soft during pregnancy and at parturition in comparison with the non-pregnant state. the purpose of the study is to evaluate the changes in consistency of the cervix during pregnancy by two methods. one is based upon the acoustical theory that the elasticity of the tissue correlates to the sound velocity (v). v = (e/rho)1/2 where e is an elastic modulus and rho is the density of the tissue. the ultrasonic velocities of 355 pregnant and 12 non-pregnant women ... | 1987 | 3295082 |
| [comparative value of immunoelectrophoresis and elisa in the serological diagnosis of human hypodermyiasis]. | 186 sera of patients with hypodermatosis were comparatively studied by immunoelectrophoresis and elisa. after establishing limits of certainty (2 distinct precipitating arcs with iep and an optical density greater than or equal to 0.8 with elisa), the comparison of obtained results shows that there is a good concordance between the two methods, but that the second, by its higher sensitiveness, allows to reveal residual antibodies during a delay after disease recovery longer than with the first. ... | 1987 | 3295704 |
| [imported cases of melioidosis and the epidemiological surveillance of its spread]. | 1987 | 3296583 | |
| vibrio cholerae metalloproteinase degrades intestinal mucin and facilitates enterotoxin-induced secretion from rat intestine. | mucin secretion in situ from rat intestinal loops was promoted more effectively by dialysed crude cholera filtrate than by an equivalent amount of purified enterotoxin. the filtrate could be rendered inactive by incubation with mixed gangliosides or passage through a gm1-affinity column, which indicated that the secretory action of the filtrate depended upon its enterotoxin component. in an effort to explain the greater potency of the filtrate, we established the presence of a metalloproteinase ... | 1987 | 3297167 |
| use of radiolabeled monoclonal anti-b1 antibody for b lymphocyte imaging in rhesus monkeys. | imaging tissues rich in b lymphocytes in man using a radiolabeled monoclonal anti-b cell antibody would be extremely useful in the clinical staging of non-hodgkins lymphomas. studies were done in rhesus monkeys using radiolabeled monoclonal anti-b1 antibody to determine the feasibility of such an approach. immunohistologic studies demonstrated that infused monoclonal anti-b1 binds in vivo with specificity to b cells in lymph nodes and spleen. the kinetics of clearance of 131i-labeled anti-b1 wer ... | 1987 | 3298171 |
| epinephrine, glucose, and lactate infusion in exercising adrenodemedullated rats. | the purpose of this study was to determine the metabolic function of the marked increase in plasma epinephrine which occurs in fasted rats during treadmill exercise. fasted adrenodemedullated (adm) and sham-operated (sham) rats were run on a rodent treadmill (21 m/min, 15% grade) for 30 min or until exhaustion. adm rats were infused with saline, epinephrine, glucose, or lactate during the exercise bouts. adm saline-infused rats showed markedly reduced endurance, hypoglycemia, elevated plasma ins ... | 1987 | 3298192 |
| [19f]-1h heteronuclear nuclear overhauser effect studies of the acyl chain-binding site of acyl carrier protein. | [19f]-1h heteronuclear difference nuclear overhauser effect experiments are performed on a sample of 5,5-difluorohexanoyl acyl carrier protein from escherichia coli. interaction of the fluorines at the 5-position of the acyl chain with protons on methyl groups of isoleucine 54 and alanine 59 is clearly indicated. the covalent attachment of the acyl chain via a prosthetic group to serine 36 and the known alpha-helix which exists from residues 36 to 51 greatly restrict the structural models which ... | 1987 | 3298246 |
| translocation of escherichia coli reca protein from a single-stranded tail to contiguous duplex dna. | duplex dna with a contiguous single-stranded tail was nearly as effective as single-stranded dna in acting as a cofactor for the atpase activity of reca protein at neutral ph and concentrations of mgcl2 that support homologous pairing. the atp hydrolysis reached a steady state rate that was proportional to the length of the duplex dna attached to a short 5' single-stranded tail after a lag. separation of the single-stranded tail from most of the duplex portion of the molecule by restriction enzy ... | 1987 | 3298251 |
| a simple method to eliminate the antigenicity of surface class i mhc molecules from the membrane of viable cells by acid treatment at ph 3. | we describe here a simple, reproducible method which specifically eliminates the antigenicity of surface class i major histocompatibility complex (mhc) molecules by acid treatment at ph 3 from the membrane of viable cells. when fresh mononuclear cells (mnc) or established cultured cell lines were treated at 4 degrees c for 2 min with citric acid buffer at ph 3 containing 1% bovine serum albumin, the antigenicity of class i mhc molecules, but not those of class ii mhc and the other non-mhc antige ... | 1987 | 3298442 |
| genetics of vibrio cholerae and its bacteriophages. | 1987 | 3299030 | |
| evidence for the involvement of atp in co-translational protein translocation. | identification of the source of energy for protein translocation across biological membranes is important in understanding the mechanism of this process. in eukaryotic cells, the tight coupling between translation and translocation and firm attachment of the secreting ribosomes to membranes, as well as theoretical calculations, have led to the suggestion that energy derived from protein synthesis is sufficient for protein translocation. on the other hand, in bacterial systems neither the attachm ... | 1987 | 3299104 |
| erg, a human ets-related gene on chromosome 21: alternative splicing, polyadenylation, and translation. | the avian acute leukemia virus e26 induces a mixed erythroid-myeloid leukemia in chickens and carries two distinct oncogenes, v-myb and v-ets. recently, a novel gene named erg, closely related to the v-ets oncogene, was identified in human colo 320 cells and the nucleotide sequence of its approximately 5.0-kilobase transcript, erg 1 was determined. in the present study, the nucleotide sequence of the alternatively spliced transcript, erg 2, was found to differ from erg 1 by a splicing event that ... | 1987 | 3299708 |
| proteolytic activity of the cowpea mosaic virus encoded 24k protein synthesized in escherichia coli. | the function of the 24-kilodalton (24k) protein encoded by cowpea mosaic virus (cpmv) has been studied by constructing a bacterial expression plasmid that contained a cloned chimeric segment consisting of partial dna copies of cpmv m-rna (including sequences coding for both capsid proteins) and b-rna (including sequences coding for the 24k protein). viral sequences were transcribed from the phage t7 promoter phi 10 of plasmid pt7-6 using t7-rna polymerase expressed from plasmid pgp1-2 present in ... | 1987 | 3300014 |
| proteolytic activity of the cowpea mosaic virus encoded 24k protein synthesized in escherichia coli. | the function of the 24-kilodalton (24k) protein encoded by cowpea mosaic virus (cpmv) has been studied by constructing a bacterial expression plasmid that contained a cloned chimeric segment consisting of partial dna copies of cpmv m-rna (including sequences coding for both capsid proteins) and b-rna (including sequences coding for the 24k protein). viral sequences were transcribed from the phage t7 promoter phi 10 of plasmid pt7-6 using t7-rna polymerase expressed from plasmid pgp1-2 present in ... | 1987 | 3300014 |
| [immunosuppression trials in type i diabetes]. | 1987 | 3302472 | |
| mitomycin-induced prophage induction in vibrio cholerae cells. | 1987 | 3133305 | |
| survival of pathogenic bacteria in environmental microcosms. | the survival times of salmonella enteritidis, pseudomonas aeruginosa, staphylococcus aureus, and vibrio cholerae in natural soil and river water samples were monitored by using various differential media. s. aureus and v. cholerae failed to survive in samples of soil, groundwater, and river water with various degrees of eutrophication. the population of the introduced s. enteritidis remained fairly constant in all three samples of soils tested. in water samples, numbers of s. enteritidis and p. ... | 1987 | 3130237 |
| association between o blood group and occurrence and severity of diarrhoea due to escherichia coli. | during studies of diarrhoea due to escherichia coli in 316 adult volunteers, abo and rh blood group determinations were done to look for differences in the occurrence or severity of illness in association with certain blood groups. in studies using heat-labile enterotoxin-producing e. coli, volunteers with o blood group had a significantly higher attack rate for diarrhoea than persons with other blood groups. in contrast, in studies with enteropathogenic or heat-stable enterotoxin-producing e. c ... | 1987 | 3127953 |
| rescue of menotrophin cycles prone to develop ovarian hyperstimulation. | in an attempt to prevent the loss of 'overstimulated cycles' associated with human menopausal gonadotrophin (hmg)-induced ovulation, oestradiol levels and ovarian follicular state were monitored in 12 women with 'overstimulated cycles' after withholding hmg for several days. human chorionic gonadotrophin (hcg) was administered when oestradiol levels were less than or equal to 1700 pg/ml and the leading follicles between 17 and 22 mm in diameter. during the withholding period follicular growth co ... | 1987 | 3122819 |
| man's place in hominoidea as inferred from molecular clocks of dna. | divergence dates among primates were estimated by molecular clock analysis of dna sequence data. a molecular clock of eta-globin pseudogene was calibrated by setting the date of divergence between catarrhini and platyrrhini at 38 million years (myr) ago. the clock gave dates of 25.3 +/- 2.4, 11.9 +/- 1.7, 5.9 +/- 1.2, and 4.9 +/- 1.2 myr ago ( +/- refers to standard error) for the separation of rhesus monkey, orangutan, gorilla, and chimpanzee, respectively, from the line leading to humans. in p ... | 1987 | 3125331 |
| renal aspergilloma--a case illustrating the problems of medical therapy. | the second documented case of renal aspergilloma due to aspergillus flavus is presented. the merits of the medical therapy that failed are discussed. pathological examination showed a nidus of aspergillus around suture material persisting from a pyelolithotomy operation 2 years before in india. we argue that this was the reason for the failure of the medical therapy. this is the first case of its kind reported. | 1987 | 3126459 |
| isolation of salmonella using three selective enrichment broths from sausages naturally contaminated. its relationship with other microorganisms that produce food toxi-infections. | recently a great number of cases of human salmonellosis has been observed, and contaminated foods are the most important source of gastro-intestinal diseases by salmonella. a comparative study of the efficiency of salmonella spp. isolation from different kinds of sausage was carried out, using selenite-cystine (sc), müller-kauffmann tetrathionate (mk) and rappaport-vassiliadis (rv/43) broths. in addition, other microorganisms that produced food toxi-infections were investigated. the percentage o ... | 1987 | 3124372 |
| [potential use of serological methods in detecting cholera toxin]. | in the study of 50 vibrio cholerae museum strains, 45 of them producing cholerigenic effect in suckling rabbits, cholera toxin, determined by means of the passive immune hemolysis (pih) test, has been detected in the supernatant of the culture fluid of only two strains: v. cholerae 569 b, a well-known producer of cholera toxin, and v. cholerae (eltor) 1310, from whose population a toxigenic variant has been obtained by selection. to study the capacity of v. cholerae for producing toxin in vitro, ... | 1987 | 3124418 |
| relationship of smoking to lifestyle factors in women. | the purposes of this research were to examine the relationship of female smoking and lifestyle factors and to assess the applicability of an index of "emancipation" to smoking status in women who live in the province of ontario. the index of "emancipation," developed by eide, was based on distinguishing women who are not following traditional roles as housewives from those who are. while reports in the literature confirm that occupational status, marital status and educational achievement are re ... | 1987 | 3424848 |
| [ultrastructural characteristics of elastogenesis in the major arteries of the canine hindlimb during leg lengthening]. | in mature dogs ultrastructural peculiarities of elastogenesis in femoral and anterior tibial arteries have been studied at various stages of the bone elongation after ilizarov method. from the end of the 1st week of distraction, metabolic activation of intimal smooth muscle cells is revealed, from the 2d week--in the middle tunic, and on the 5th-6th week--fibroblasts of adventitia of the arteries investigated, directed to biosynthesis of intracellular predecessors of elastin and microfibrils of ... | 1987 | 3426417 |
| specific antibodies to cholera toxin in rabbit milk are protective against vibrio cholerae-induced intestinal secretion. | breast feeding helps to protect the nursing infant against infectious diarrhoeas, but the relative importance of antibodies compared with other components present in milk is unsettled. in order to aid in resolving this issue we evaluated the ability of milk, collected from rabbits not immunized or immunized enterally during pregnancy with toxinogenic, live vibrio cholerae, to inhibit water secretion induced by v. cholerae in rat ileal loops. non-immune milk was not inhibitory, whereas immune mil ... | 1987 | 3443455 |
| [the use of the gene hybridization method for the identification of epidemiologically dangerous strains of cholera vibrios]. | using the labeled dna fragments containing the genes for cholera toxin the strains of cholera vibrios were studied for the presence of cholera toxin genes. vibrio cholerae strains isolated from natural water reservoirs under the favourable epidemic situation do not contain the cholera toxin genes. the dna hybridization method was compared with other methods used in research and practical work for estimation of epidemic importance of cholera vibrios. | 1987 | 3444442 |
| visual acuity of human infants at scotopic, mesopic and photopic luminances. | visual acuity of 2-month-old infants and adults was measured between -2.6 and +2.7 log10 cd/m2 using standard psychophysical techniques. the acuity-vs-luminance curve was similar for infants and adults: acuity improved with increasing luminance until 0.0 log cd/m2, and was constant above that luminance. however, the infant curve was slightly shallower than the adult curve, and infant acuity was over 3.5 octaves poorer than adult acuity at all luminances. the infant acuity-vs-luminance curve was ... | 1987 | 3445474 |
| visual latency of ganglion x- and y-cells: a comparison with geniculate x- and y-cells. | visual response latencies and rise times of x and y ganglion cells recorded in the optic tract of anaesthetized, paralyzed cats were measured during repeated stimulation with sinusoidal gratings. these measures were compared with visual latencies and rise times of x- and y-cells in the dorsal lateral geniculate nucleus. measurements were restricted to individual trials on which the instantaneous discharge rate exceeded a criterion amplitude defined in terms of the statistics of the baseline acti ... | 1987 | 3445476 |
| the mesangial cell culture: a tool for the study of the electrophysiological and pharmacological properties of the glomerular mesangial cell. | cultured rat glomerular mesangial cells (mc) were evaluated as a tool for reliable electrophysiological measurements as well as for fluorimetric determinations of intracellular ca++. they had a resting potential similar to that observed in cultured vascular smooth muscle cells (vsmcs), in vsmcs of mouse kidney arterioles, or in glomerular--presumably mesangial--cells of kidney slices. the comparison with the other cell types was carried out in order to look for features distinguishing them from ... | 1987 | 3446561 |
| [comparison of nucleolar organizer regions (nors) on chromosomes of cultured lymphocytes from cancer patients, high risk cancer family members and normal subjects]. | using the technique of ag-nors, the number of silver-stained nors on chromosomes of the peripheral lymphocytes from 10 cancer patients, 10 high risk cancer family members and 10 normal individuals was compared. it was found that the total mean value of ag-nors was 5.46 +/- 0.00/per cell in cancer patients, 5.62 +/- 0.01/per cell in high risk cancer family members and 7.03 +/- 0.07/per cell in normal subjects. the total mean value of ag-nors was markedly lower in the first two groups than in the ... | 1987 | 3447861 |
| lactation response to short-term abomasal infusion of choline, inositol, and soy lecithin. | five lactating holstein cows averaging 13 wk postpartum were used in a latin square design to examine the effect of daily abomasal infusion of choline (22 g), myo-inositol (37 g), soy oil (325 ml), or crude soy lecithin (900 ml) on lactation performance. dry matter intake was reduced by infusion of soy lecithin as compared with infusion of water (18.1 and 21.1 kg/d, respectively). plasma beta-hydroxybutyrate concentration was increased when cows received the myo-inositol or soy lecithin infusion ... | 1987 | 3448106 |
| the role of catecholamines and glucagon on serum and liver metallothionein response to restraint stress. | the role of glucagon and catecholamines on serum and liver metallothionein (mt) concentrations in basal and stress conditions has been studied. glucagon showed no effect on serum mt either in basal (unstressed) or stress (20 hours of restraint) conditions. in contrast, glucagon administration increased both unstressed and stressed levels of liver mt. no effect of the alpha + beta-catecholamine blocker labetalol on serum mt levels was observed in unstressed rats. however, the administration of la ... | 1987 | 3448709 |
| a dna probe to distinguish the species anopheles quadriannulatus from other species of the anopheles gambiae complex. | dna probes used previously to distinguish the species anopheles gambiae sensu stricto, an.arabiensis, an.melas and an.merus were tested against an.quadriannulatus. using these dna probes, an.gambiae s.s. and an.quadriannulatus were indistinguishable. a genomic library was constructed for an.quadriannulatus. differential screening of this genomic library with an.gambiae s.s. and an.quadriannulatus genomic dnas identified a species-specific, repeated dna sequence. when used as a hybridization prob ... | 1987 | 3450008 |
| characterization of vibrio cholerae 01 recently isolated in bangladesh. | 91 strains of vibrio cholerae o1, isolated in bangladesh in january 1986, were examined for their biological behaviour and sensitivity to 6 antimicrobial agents. biotyping indicated that 60 of the isolates belonged to the classical biotype and 31 to the el tor biotype. 21 el tor strains revealed beta-haemolysis on blood agar plates, but only 8 showed complete haemolysis in broth. serotyping indicated 79 ogawa, 10 inaba, and 2 hikojima. phage typing showed that all classical vibrios belonged to m ... | 1987 | 3450016 |
| role of sialic acid in synaptosomal transport of amino acid transmitters. | active, high-affinity, sodium-dependent uptake of gamma-aminobutyric acid and of the acidic amino acid d-aspartate was inhibited by pretreatment of synaptosomes with neuraminidase from vibrio cholerae. inhibition was of a noncompetitive type and was related to the amount of sialic acid released. the maximum accumulation ratios of both amino acids (intracellular [amino acid]/extracellular [amino acid]) remained largely unaltered. treatment with neuraminidase affected neither the synaptosomal ener ... | 1987 | 3470752 |
| immunotoxins to a human melanoma-associated antigen: resistance to pokeweed antiviral protein conjugates in vitro. | pokeweed antiviral protein (pap) from the summer leaves of phytolacca americana was purified and conjugated via n-succinimidyl-3-(2-pyridyldithio)propionate to 9.2.27 anti-melanoma antibody to a glycoprotein-proteoglycan complex. the conjugate was highly potent (50% inhibition dose of 5 x 10(-11)-10(-13) m on antigen-positive melanoma) and highly selective (5 x 10(-8) on antigen-negative melanoma). human melanoma cells were selected for resistance to in vitro killing of the pap conjugate by cycl ... | 1987 | 3473251 |
| [isolation of the r'his plasmids of vibrio cholerae]. | v. cholerae strain vt5104 capable of donor activity in conjugation has been constructed by the genetic technique based on plasmid rp4::mucts62 integration into v. cholerae chromosome due to plasmid homology with mucts62 inserted into the chromosome. the gene for histidine synthesis has been mobilized and transferred into the recipient cells from vt5104 donor. the conjugants obtained are able to efficiently transfer his+ gene included into the plasmid structure in conjugation with eltor recipient ... | 1987 | 3474038 |
| salt-induced plasma factor that inhibits platelet thromboxane a2 release and renal prostaglandin e2 production in rats. | this study examined the relationship between a plasma factor (or factors) that inhibits the release of thromboxane a2 from platelets and excessive salt intake in rats. the plasma factor, termed platelet inhibitory factor, was also characterized. the release of thromboxane a2 from thrombin-activated platelets was reduced in wistar rats that were uninephrectomized and given 2% saline for a week, but not in rats with acute volume expansion. platelet inhibitory factor was extracted from the plasma o ... | 1987 | 3474209 |