Publications

reversal by cyclic amp of the urea-induced inhibition of synthesis of a catabolite-repressible enzyme in vibrio cholerae.	low concentrations of urea, which did not inhibit the synthesis of the catabolite nonrepressible enzyme alkaline phosphatase in vibrio cholerae, or markedly affect its overall growth, specifically inhibited the expression of the tryptophanase operon in a temperature-dependent manner. however, in contrast to what is found in escherichia coli, this urea-induced inhibition of tryptophanase synthesis in v. cholerae could be almost completely relieved by exogenously added cyclic amp. the possible mec ...	1987	2833565
[genetic transformation of somatic cells. xv. production of transgenic mice containing the gene for the envelope protein of the human hepatitis b virus].	transgenic mice containing in somatic (liver) cells a gene encoding hepatitis virus b surface antigen (hbsag) were obtained and analysed. about 10(4)-10(5) copies of circular plasmid psh21 with hbsag gene under the control of the sv40 early promoter were microinjected into the cytoplasm (pronucleus region) of mouse zygotes. 10 of the 24 animals isolated after this treatment contained 1 to 5 copies of the hbsag gene per genome of hepatocytes (7 males and 3 females). the southern analysis of dna f ...	1987	2830691
[genetic transformation of somatic cells. xv. production of transgenic mice containing the gene for the envelope protein of the human hepatitis b virus].	transgenic mice containing in somatic (liver) cells a gene encoding hepatitis virus b surface antigen (hbsag) were obtained and analysed. about 10(4)-10(5) copies of circular plasmid psh21 with hbsag gene under the control of the sv40 early promoter were microinjected into the cytoplasm (pronucleus region) of mouse zygotes. 10 of the 24 animals isolated after this treatment contained 1 to 5 copies of the hbsag gene per genome of hepatocytes (7 males and 3 females). the southern analysis of dna f ...	1987	2830691
l929 cells infected with temperature sensitive mutants of vesicular stomatitis virus: virus replication is necessary for induction of changes in membrane permeability.	infection of l929 murine cells with vesicular stomatitis virus (vsv) results in inhibition of host protein synthesis and appearance of membrane alterations at a time when cells are still actively engaged in viral protein synthesis. vsv temperature-sensitive (ts) mutants have been used to explore the role(s) played by the virus-coded proteins in the genesis of these effects. cells were infected with each of five ts mutants representing the known complementation groups of vsv indiana serotype, and ...	1987	2827608
l929 cells infected with temperature sensitive mutants of vesicular stomatitis virus: virus replication is necessary for induction of changes in membrane permeability.	infection of l929 murine cells with vesicular stomatitis virus (vsv) results in inhibition of host protein synthesis and appearance of membrane alterations at a time when cells are still actively engaged in viral protein synthesis. vsv temperature-sensitive (ts) mutants have been used to explore the role(s) played by the virus-coded proteins in the genesis of these effects. cells were infected with each of five ts mutants representing the known complementation groups of vsv indiana serotype, and ...	1987	2827608
calcium-permeable channels in vascular smooth muscle: voltage-activated, receptor-operated, and leak channels.		1987	2850612
has cyclosporine really relegated pretransplant blood transfusions to therapeutic obsolescence?		1987	2856274
a detailed study of cytomegalovirus infections in the first 160 heart and heart/lung transplant recipients at papworth hospital, cambridge, england.		1987	2856284
complete nucleotide sequence coding for rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase derived from a cdna clone.	cdna clones for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase were isolated from rat liver expression libraries in lambda gt11 by antibody, oligonucleotide, and cdna screening. one 1860 bp long clone contained a full-length nucleotide sequence coding for the 470 amino acids of each of the two identical subunits of the bifunctional enzyme. this clone also contained untranslated sequences, one 173 bp long upstream from the atg start codon and one 271 bp long downstream from the tga stop cod ...	1987	2856848
a major outer membrane protein in vibrio cholerae is maltose-inducible.		1987	2849017
antiviral chemotherapy and prophylaxis of viral respiratory disease.	respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ...	1987	2446820
effects of substance p, neurokinin a and calcitonin gene-related peptide in human skin and their involvement in sensory nerve-mediated responses.	the effects evoked by intradermal injections of substance p (sp), neurokinin a (nka) or calcitonin gene-related peptide (cgrp) were studied in 51 non-atopic subjects. sp and nka produced flare and weal, and cgrp produced an indurated erythema. the reactions to sp were strong, the flare being maximal 3-5 min after injection and the weal after 10-15 min. nka evoked a much weaker flare and a slightly weaker weal than did sp. cgrp produced a prominent long-lasting, indurated erythema with pseudopod ...	1987	2446892
[similarity of vibrio alginolyticus, v. cholerae and other vibrio species with respect to the structure of their flagellar apparatus and ribosomal 5s-rna].	electron microscopic analysis of basal bodies of the flagella vibrio alginolyticus revealed a structure composed of four discs. the diameters of two discs localized in the cytoplasmic membrane appeared to be twice as little as those of the other two discs. in this respect the basal body of v. alginolyticus resembles that of v. cholerae. the 5s sequence of ribosomal rna from v. alginolyticus appeared to be similar to those of v. cholerae, v. harveyi and some other vibrios. comparison of 5s-rna se ...	1987	2434145
[effect of kontrikal on the dynamics of proteolytic system indices in postischemic toxemia].	it was shown in wistar male rats that the development of tourniquet shock was followed by an increase of proteolytic activity in the blood by 3 times, activity of aspartate aminotransferase (ast) by 3 times, that of alanine aminotransferase (alt) by 6 times, contents of urea and residual nitrogen by 2.5-3 times; level of alpha 1-protease inhibitor (alpha 1-pi) decreased by 4 times and that of alpha 2-macroglobulin (alpha 2mg) by 2.5 times. at administration of contrykal (10,000 u/kg) proteolytic ...	1987	2435573
the presence of the 30 nm filament structure of chromatins in intact chicken erythrocytes observed by 31p nmr.	a 31p nmr spectrum of chicken erythrocyte chromatins in vivo was obtained. the spectrum was characterized by examining the spectra of isolated nuclei and nondigested chromatins under various conditions. the results have shown that most chromatins in chicken erythrocyte nuclei assume the 30 nm filament structure as a second step of condensation of dna in vivo.	1987	2435577
sodium-channel inhibitors produced by enteropathogenic vibrio cholerae and aeromonas hydrophila.		1987	2436014
lectin binding to human endometrial hyperplasias and adenocarcinoma.	arias-stella change, cystic glandular hyperplasia, adenomatous hyperplasia, atypical hyperplasia, and adenocarcinoma of the human endometrium were stained with different fluorescein-conjugated lectins. the lectins used were concanavalin a, triticum vulgaris, arachis hypogaea, glycine maximum, dolichos biflorus, ulex europaeus, and ricinus communis. the results showed that the lectin staining of arias-stella change differed from the other conditions and resembled more closely the staining propert ...	1987	2447025
chinese hamster ovary cell lysosomes rapidly exchange contents.	we have used cell fusion to address the question of whether macromolecules are rapidly exchanged between lysosomes. donor cell lysosomes were labeled by the long-term internalization of the fluid-phase pinocytic markers, invertase (sucrase), lucifer yellow, fitc-conjugated dextran, or texas red-conjugated dextran. recipient cells contained lysosomes swollen by long-term internalization of dilute sucrose or marked by an overnight fitc-dextran uptake. cells were incubated for 1 or 2 h in marker-fr ...	1987	2447096
a protein preparation from mycobacterium kansasii culture filtrate has biological activity similar to that of hcg and cholera toxin in human cell lines.	a protein preparation, from culture filtrates from a strain of mycobacterium kansasii (mk precipitate), which cross reacts with antibodies to human chorionic gonadotropin (hcg) beta subunit and antibodies to cholera toxin beta subunit, has been isolated. a tissue culture assay was used to detect the ability of this preparation to affect the antiviral activity of interferons and to visualize changes in cell shape and cell-cell contact caused by this preparation. a cholera toxin containing precipi ...	1987	2447470
different types of monoclonal antibodies to ogawa-specific and group-specific antigens of vibrio cholerae o1.	nine monoclonal antibodies to ogawa-specific antigenic determinants of vibrio cholerae o1 and seven monoclonal antibodies to the ogawa-inaba common antigenic determinants were obtained. specificities and reactivities were examined by slide or microdilution agglutination methods, along with enzyme-linked immunosorbent assays and immunoblotting analysis. in both the ogawa-specific and ogawa-inaba common groups, it was revealed that there were two types of antibodies. one type showed strong aggluti ...	1987	2448333
studies on the vibrio cholerae mucinase complex. ii. specific neuraminidase activity measured histochemically in a goblet cell assay.	the activity of neuraminidase prepared from the mucinase complex of vibrio cholerae was measured by a new, semi-quantitative goblet-cell assay. the counts of normal, alcianophilic, sialomucin-containing goblet cells (purple-stained) and neutral mucosubstance-containing goblet cells (magenta-stained) in serial sections of ileum were compared before and after neuraminidase treatment. the procedure provides a more natural assessment of the action of v. cholerae neuraminidase on the viscous intestin ...	1987	2438408
isolation of novel human retrovirus-related sequences by hybridization to synthetic oligonucleotides complementary to the trna(pro) primer-binding site.	synthetic oligonucleotides complementary to putative retroviral primer-binding sites were used as hybridization probes to detect novel retroviruslike sequences. an 8.1-kilobase element with structural features of a retroviral provirus was isolated from a human genomic library by this approach. nucleotide sequence analysis of its 600-base-pair long terminal repeats revealed characteristic motifs known as regulatory signals for rna polymerase ii transcription: ccaat, tata, and attaaa. in addition, ...	1987	2438428
epiglycanin-immunoreactive glycoproteins in mouse fetal tissues and fetal cells in culture.	fetal tissues from time-pregnant female a/j mice of 16- and 19-day pregnancies and from neonates 1 day after birth, as well as from fetal cells in culture, absorbed significant amounts of anti-epiglycanin antibody. detergent-solubilized glycoproteins, with epiglycanin activity, from fetal tissues and cells were separated by polyacrylamide gel electrophoresis, and the protein bands electroblotted onto a nitrocellulose gel. after the antigens were labeled with rabbit anti-epiglycanin antiserum and ...	1987	2438443
a mechanistic interpretation of the action of toxin ii from anemonia sulcata on the cardiac sodium channel.	cardiac sodium channels, modified by anemonia sulcata toxin ii, have been analyzed by the patch-clamp method. the open state of the modified sodium channels proved to be prolonged highly significantly and reopening from a closed state denoted c-state frequently occurred, interrupted by silent periods, denoted i-state. activation from the c-state was apparently not affected by toxin action, whereas activation from the i-state was markedly prolonged. upon higher depolarizations toxin-induced s ...	1987	2440476
no evidence on significant roles of the prostaglandin-thromboxane and kallikrein-kinin system in the antihypertensive effect of mk 421 in rats made hypertensive by norepinephrine or vasopressin.	inhibition of angiotensin converting enzyme by mk 421 (6 mg/kg/day ip) induced a significant increase in urinary kinin excretion in norepinephrine-infused rats (1.8 mg/kg/day ip), whereas it had no effect on urinary prostaglandin e2 excretion. in contrast, mk 421 did not induced any significant changes in urinary kinin and prostaglandin e2 excretion in vasopressin-infused rats (7.2 u/kg/day ip). the simultaneous administration of indomethacin (10 mg/kg/day sc), oky 046 (12 mg/kg/day sc) or aprot ...	1987	2440625
[heterogeneity of sperm immobilizing antibodies in sera of sterile women].	among 126 sterile women, 8 patients indicated sperm immobilizing (si) antibodies in their sera, while 13 and 57 patients showed positive results in sperm agglutination (sa) test and indirect immunofluorescence (if) tests, respectively. these results suggest that the antisperm antibodies detected by different methods are not identical and seem to be raised to different epitopes of sperm antigens. si antibodies were absorbed not only with human spermatozoa, but also with human seminal plasma (hsp) ...	1987	2440964
contradictory responses in induction of delayed type hypersensitivity in orally immunized mice.	induction of delayed type hypersensitivity (dth) in mice fed with sheep red blood cells (srbc) and live vibrio cholerae (v. cholerae) both forcedly and ad lib was comparatively investigated. although suppression of dth to srbc or v. cholerae was induced in mice fed forcedly for 1 or 2 weeks, mice fed ad lib could produce the positive footpad reactions to antigens. furthermore, the suppression of dth in forcedly fed mice showed an antigenic specificity. these observations indicated that the induc ...	1987	2442393
topological mapping of complement component c9 by recombinant dna techniques suggests a novel mechanism for its insertion into target membranes.	cdna molecules coding for mouse and trout c9 have been isolated and the derived amino acid sequences compared with that of human c9. regions of high homology between the closely related species (mouse and human) correlate with putative domains in the protein structure supporting a model of c9 having five globular domains. comparison between the more distant species (trout and human) suggests regions of particular importance to c9 structure and function. in addition the three related sequences al ...	1987	2443347
translation framing code and frame-monitoring mechanism as suggested by the analysis of mrna and 16 s rrna nucleotide sequences.	protein coding sequences carry an additional message in the form of a universal three-base periodical pattern (g-non-g-n)n, which is expressed as a strong preference for guanines in the first positions of the codons in mrna and lack of guanines in the second positions. this periodicity appears immediately after the initiation codon and is maintained along the mrna as far as the termination triplet, where it disappears abruptly. known cases of ribosome slippage during translation (leaky frameshif ...	1987	2443708
isolation and structural analysis of the escherichia coli trp leader paused transcription complex.	transcription pausing is a key step in many prokaryotic transcription attenuation mechanisms. pausing is thought to occur when an rna hairpin forms near the 3' end of a growing transcript. we report here the isolation of the trp leader paused transcription complex containing a defined 92-nucleotide nascent transcript. digestion of isolated paused complexes with rnase t1 suggests that the trp leader rna hairpin designated 1:2 forms in the paused transcription complex. the transcription factor nus ...	1987	2443722
[control of early pregnancies by vaginal sonography].	151 patients with suspected early pregnancies between 5 to 10 weeks were examined by transvaginal section scanning. pregnancies could be verified as early as 16 days after conception while the first heart beat actions were seen after 21 to 22 days. in early pregnancies where heart actions could not be shown at first examination a second look was taken after the 6. week dated from conception. beta-hcg-units were found in a range of 59 to 55,000 mu/ml. 3 pregnancies in the 5th, 6th, respectively 7 ...	1987	2444038
a physical map of the chromosomal region determining o-antigen biosynthesis in vibrio cholerae o1.	we have previously described the cosmid cloning of the genes determining the biosynthesis of the inaba and ogawa o-antigens of the lipopolysaccharides of vibrio cholerae o1 (manning et al., 1986). by southern hybridization analysis of chromosomal and cosmid dna, and heteroduplex analysis between the clones we have been able to precisely define the region of contiguous chromosomal dna in the vicinity of the o-antigen-encoding region. these data and comparison of end points of clones and of deleti ...	1987	2444496
analysis of human antibody response to cholera vaccination by epstein barr virus transformation and human-human hybridoma production.	the response of epstein barr virus transformable b lymphocytes to v. cholerae serotypes inaba and ogawa was assessed at various periods of time after primary and secondary immunization. in both cases, 100% of cells laid down at 10(4)/well gave a very strong positive reaction with either serotype one week after immunization. further analysis indicated that general polyclonal activation by the bacterial lps was not a major feature since the reactivity with other bacterial antigens was weak. human ...	1987	2444705
[in vitro preparation of enterotoxigenic species of vibrio cholerae 01 from nontoxigenic cultures of cholera and nag vibrios].		1987	2444761
antigenic determinants of the cholera/coli family of enterotoxins.	hybridoma-derived monoclonal antibodies were raised to enterotoxins of the cholera family and to chimeric b-subunit proteins in which individual amino acid residues of a heat-labile, cholera-related enterotoxin from an escherichia coli strain of porcine origin (p-lt) were substituted with corresponding residues from such an enterotoxin from an e. coli strain of human origin (h-lt). single amino acid substitutions were found to have profound effects on the physicochemical behavior of the proteins ...	1987	2446368
[effect of dna and rna in foods on serum uric acid concentration in the human].	nutritional experiments with formula diets show, that dna taken orally raises the serum uric acid concentration only half as much as does the same quantity of rna. in evaluating the suitability of foods used in the diet of patients with gout, however, food is only judged by its total purine-n content. in commercially available raw food the total purine content was determined enzymatically as uric acid. in the same samples the content of rna, dna, nucleotides, nucleosides and bases was determined ...	1987	2446437
evolution and development of the outer acrosomal membrane (oam) and evidence that acrosin-inhibitors are proteins of the oam.	an antiserum to the purified porcine outer acrosomal membrane (oam) was raised in rabbits and the igg fraction isolated by ammonium sulphate precipitation and ion exchange chromatography. the antibodies reacted exclusively with the acrosomal cap of the sperm head as revealed by indirect immunofluorescence. in addition they cross-reacted not only with the acrosomal part of the spermatozoa of all mammalian species tested (bull, horse, rabbit, rat, mouse, hamster, mole, antelope, monkey, man) but a ...	1987	2446533
antibodies to the insulin receptor as tools in the study of receptor structure and function.		1987	2446553
definition of myasthenogenic sites of the human acetylcholine receptor using synthetic peptides.	experimental autoimmune myasthenia gravis (eamg) and antibodies that modulate achrs from cultured human muscle are induced by a disulfide-looped peptide comprising the human acetylcholine receptor (achr) alpha-subunit residues 125-147 (h alpha 125-147). to delineate the essential antigenic requirements for induction of eamg by this peptide, a series of peptides was synthesized: (a) a nonlooped analog (cys 128 replaced by ser) stimulated modulating autoantibodies, induced eamg, and bound antibodi ...	1987	2446556
distributions of certain neuropeptides in the primate thalamus.	the distributions of fibers and terminals immunoreactive for somatostatin (srif), neuropeptide y (npy), substance p (sp) and cholecystokinin octapeptide (cck), were studied in the diencephalon of cynomolgus monkeys. immunoreactivity for all 4 peptides is found in extrinsic afferent fibers innervating the dorsal thalamus, ventral thalamus and epithalamus. the distributions of such fibers are more extensive than previously described and include many relay nuclei in their zones of terminations. sp ...	1987	2446708
calcium channels and excitation-contraction coupling in cardiac cells. ii. a pharmacological study of the biphasic contraction in guinea-pig papillary muscle.	biphasic contractions were obtained in guinea-pig papillary muscle by inducing partial depolarization in k+-rich solution (17 mm) in the presence of 0.3 microm isoproterenol. mn2+ ions inhibited the two components of contraction in a similar way. nifedipine and particularly cd2+ ions specifically inhibited the second component of contraction. isoproterenol and bay k 8644 markedly increased the amplitude of the second component (p2) of contraction. nevertheless, a moderate positive inotropic effe ...	1987	2446724
purification and partial characterization of fimbriae of vibrio cholerae o1.	fimbriae of vibrio cholerae o1 were purified from a strain of the classical biotype, inaba serotype (bgd 17), and a strain of the el tor biotype, ogawa serotype (k23), grown on tcg agar medium by the following procedure; homogenization of the cell suspension to detach fimbriae, ultracentrifugation to remove remaining cells and their debris, concentration of the supernatant containing fimbriae with ultrafiltration, and 20 to 40% sucrose linear gradient centrifugation of the concentrated material. ...	1987	2448968
vibrio fluvialis: a new serogroup (19) possessing the inaba factor antigen of vibrio cholerae o1.	a serogroup of vibrio fluvialis possessing the c (inaba) antigen but not the b (ogawa) nor a antigen of v. cholerae o1 is described. the o-antigen of this serogroup was identical with that of bioserogroup 1875-variant of a marine vibrio species. as the o-antigen of this serogroup was not agglutinated by any of o-antisera for the 18 serogroups of v. fluvialis already recognized, it was designated o-serogroup 19 of this species.	1987	2455085
immunoglobulins in bile and serum of the rabbit associated with protection after vibrio cholerae infection and vaccination.	previous studies have shown that cholera, as well as protective immunity against infection with vibrio cholerae, can be induced in the rabbit. this protection is long-lasting (up to 30 months) and is characterized on challenge by rapid, symptom-free disappearance of v. cholerae from the intestine; we therefore believe this to be vibriocidal protection. in this study, we analysed the humoral and secretory immune response against various subcellular v. cholerae components in vibriocidally protecte ...	1987	2462153
comparison of solutions for extracting ck, ak, and ld from brain samples.		1987	2826051
tetanus toxin binds with high affinity to neuroblastoma x glioma hybrid cells ng 108-15 and impairs their stimulated acetylcholine release.	differentiated neuroblastoma x glioma hybrid cells ng 108-15 express on their surface specific binding sites for tetanus toxin. 450 sites/cell with a kd of 2 x 10(-11) m were found under "physiological" conditions of ph and salt concentrations. a hill coefficient of 1.1 indicated noncooperative binding. specific binding of 125i-toxin to its sites could be prevented either by preincubation of the toxin with a neutralizing monoclonal antibody or by pretreatment of the cells with neuraminidase (vib ...	1987	2826418
characterization and restriction analysis of the p sex factor and the cryptic plasmid of vibrio cholerae strain v58.	the p plasmid of vibrio cholerae is a derepressed sex factor restricted to v. cholerae and has been shown to express surface exclusion. we have isolated the plasmids of strain v58 and have found that in addition to p, two further cryptic plasmids are also present. p has a size of 68 kb as determined by both electron microscopy and restriction endonuclease analysis. these other plasmids are 34 and 4.7 kb in size. restriction maps of p and the larger cryptic plasmid have been determined. it has be ...	1987	2827202
[classification of spot-shaped lung changes by texture analysis].	classification of opacities in pneumoconiosis was accomplished by textural analysis in digitised chest x-rays. a good discrimination was achieved by a set of 10 parameters. these texture measures were computed by algorithms for edge detection, local extremes, difference statistics, the co-occurrence matrix and the power spectrum. the classes of the training set were classified correctly at 99%. a test set comprising additional classes that were not contained in the training set, was classified a ...	1987	2827257
mucosal antibody response of calves after oral and intrabronchial administration of rotavirus.		1987	2827448
regulation of nicotinic acetylcholine receptor phosphorylation in rat myotubes by forskolin and camp.	the nicotinic acetylcholine receptor (acchor) from rat myotubes prelabeled in culture with [32p]orthophosphate was isolated by acetylcholine affinity chromatography followed by immunoaffinity chromatography. under basal conditions, the nicotinic acchor was shown to be phosphorylated in situ on the beta and delta subunits. regulation of acchor phosphorylation by camp-dependent protein kinase was explored by the addition of forskolin or camp analogues to prelabeled cell cultures. forskolin, an act ...	1987	2819883
tn602: a naturally occurring relative of tn903 with direct repeats.	we report the characterization of tn602, a transposon encoding resistance to kanamycin and related aminoglycosides present on the r-plasmid pgd10. tn602 is highly homologous to the previously characterized tn903, present on the r-plasmid r6, in that it consists of a gene for aminoglycoside-phosphotransferase-3'-i (homologous to that of tn903) flanked by copies of an is-element homologous to is903. tn602 differs from tn903 in the following respects: the flanking is-elements (is602) are in direct ...	1987	2819910
iga multiple myeloma coexistent with atypical adult t-cell leukemia.	we report a 65-yr-old male with adult t-cell leukemia (atl) who developed multiple myeloma (mm) concomitantly. skin lesions and peripheral leukocytosis were noted during the 5-yr observation period. there was abnormal lymphocytosis with indented or lobulated nuclei in the peripheral blood and in the bone marrow. the neoplastic cells reacted with monoclonal antibodies, okt3, okt4, okia1 and anti-tac. his serum was positive for the antibodies to atl-associated antigens. human t-cell leukemia virus ...	1987	2820787
iga multiple myeloma coexistent with atypical adult t-cell leukemia.	we report a 65-yr-old male with adult t-cell leukemia (atl) who developed multiple myeloma (mm) concomitantly. skin lesions and peripheral leukocytosis were noted during the 5-yr observation period. there was abnormal lymphocytosis with indented or lobulated nuclei in the peripheral blood and in the bone marrow. the neoplastic cells reacted with monoclonal antibodies, okt3, okt4, okia1 and anti-tac. his serum was positive for the antibodies to atl-associated antigens. human t-cell leukemia virus ...	1987	2820787
the molybdenum-pterin binding protein is encoded by a multigene family in clostridium pasteurianum.	there are three variants of the molybdenum-pterin binding protein (mop) encoded by three distinct genes in clostridium pasteurianum. nucleotide sequence analysis shows that the three mop genes have greater than 90% homology at the nucleotide level. upstream from the coding region of each mop gene are potential promoter consensus sequences. analysis of mop purified from cells grown under nitrogen-fixing conditions indicates all three genes are expressed. sequence analysis of the three mop genes a ...	1987	2820842
antibodies to polymerized human serum albumin in acute and chronic liver disease.	since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ...	1987	2820857
antibodies to polymerized human serum albumin in acute and chronic liver disease.	since antibodies to polyalbumin have been noted to occur in patients with hepatitis or cirrhosis, we investigated sera from 219 patients with a variety of acute and chronic liver diseases with and without hbsag using an elisa. the prevalence of circulating polyalbumin antibodies was shown to be significantly increased over that of healthy controls (6.7%) in patients with autoimmune chronic active hepatitis (67%), acute viral hepatitis (41%) and fulminant hepatic necrosis (38%), but such antibodi ...	1987	2820857
overproduction and purification of the tn3 transposase.	the tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing escherichia coli strain harboring a transposase overproducer plasmid. this accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpr); efficient translation caused by a mutation within the shine-dalgarno (sd) sequence, the dosage effect of the increased plasmid copy number resulting from deletion of the rom gene of ...	1987	2820953
overproduction and purification of the tn3 transposase.	the tn3 transposase accumulated to about 4% of total cell protein in a minicell-producing escherichia coli strain harboring a transposase overproducer plasmid. this accumulation of the transposase seems to be due to four factors: derepression of transcription resulting from inactivation of the repressor gene (tnpr); efficient translation caused by a mutation within the shine-dalgarno (sd) sequence, the dosage effect of the increased plasmid copy number resulting from deletion of the rom gene of ...	1987	2820953
a new sv40-based vector developed for cdna expression in animal cells.	a useful vector, page103, has been developed for the expression of cdna in animal cells using the simian virus 40 (sv40) expression signals. cdna could be expressed easily by inserting it into the multiple cloning sites (hindiii, sali/acci, xbai, bamhi, smai/xmai, kpni/asp718, saci and ecori) of the vector, which are located between the sv40 early promoter and the sv40 early rna processing signals for splicing and polyadenylation. in addition to the above transcription unit, page103 contains the ...	1987	2820955
a new sv40-based vector developed for cdna expression in animal cells.	a useful vector, page103, has been developed for the expression of cdna in animal cells using the simian virus 40 (sv40) expression signals. cdna could be expressed easily by inserting it into the multiple cloning sites (hindiii, sali/acci, xbai, bamhi, smai/xmai, kpni/asp718, saci and ecori) of the vector, which are located between the sv40 early promoter and the sv40 early rna processing signals for splicing and polyadenylation. in addition to the above transcription unit, page103 contains the ...	1987	2820955
isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster.	previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ...	1987	2820958
isotopic hybrids of nitrogenase. mössbauer study of mofe protein with selective 57fe enrichment of the p-cluster.	previous mössbauer and epr studies of the mofe protein (approximately 30 fe and 2 mo) of nitrogenase have revealed the presence of two unique clusters, namely, the p-clusters (presumably of the fe4s4 type) and the molybdenum- and iron-containing cofactors (or m-clusters). mössbauer components d (approximately 10-12 fe) and fe2+ (approximately 4 fe) represent subsites of the p-clusters while component s (approximately 2 fe) appeared to belong to a separate, unidentified cluster. in order to refin ...	1987	2820958
renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c.	renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ...	1987	2822007
renal dipeptidase is one of the membrane proteins released by phosphatidylinositol-specific phospholipase c.	renal dipeptidase (dehydropeptidase-i, ec 3.4.13.11) was released from pig kidney membrane preparations by treatment with phosphatidylinositol-specific phospholipase c from staphylococcus aureus and bacillus thuringiensis and a phospholipase c preparation from bacillus cereus to a similar extent as alkaline phosphatase. endopeptidase-24.11 and aminopeptidase n were not released by this treatment. after treatment of the membrane fraction with the s. aureus phospholipase c the dipeptidase was conv ...	1987	2822007
cloning and expression of the salmonella enterotoxin gene.	this report examines the genetic basis for salmonella typhimurium q1 enterotoxin production. a 918-base-pair xbai-hincii fragment of plasmid pjm17, composed of cholera toxin (ct) coding sequences (ctxab), was used as a gene probe. with this probe, the s. typhimurium enterotoxin was identified on a 6.3-kilobase ecori-psti fragment of chromosomal dna from plasmidless strain q1. we cloned this 6.3-kilobase fragment into escherichia coli rr1. the genetic map of the cloned salmonella enterotoxin (stx ...	1987	2822664
dependence of an adenosine-activated potassium current on a gtp-binding protein in mammalian central neurons.	neurons in hippocampal and striatal cell cultures respond to adenosine with an inhibitory potassium current. this response disappears during whole-cell patch-clamp recording in which the cell is filled with minimal saline. we have found that this loss of sensitivity to adenosine can be prevented by including 100 microm gtp in the patch electrode filling solution. gdp is less effective than gtp in supporting the adenosine response, while gmp has little, if any, effect. treatments known to inhibit ...	1987	2822865
synthesis of protein in intestinal cells exposed to cholera toxin.	the mechanism by which cyclic adenosine monophosphate (amp), formed by intestinal epithelial cells in response to cholera toxin, ultimately results in alterations in water and electrolyte transport is poorly understood. several studies have indicated that inhibitors of transcription or translation block much of the transport of ions and water in the intestine and edema formation in tissue elicited by cholera toxin. data presented in this study confirmed the inhibitory effects of cycloheximide on ...	1987	2823275
pulmonary complications of aids: henry ford hospital experience between january 1984 and july 1986.		1987	2824409
functional homology of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase, phosphoglycerate mutase, and 2,3-bisphosphoglycerate mutase.	the bisphosphatase domain of the rat liver 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase has been shown to exhibit a structural similarity to yeast phosphoglycerate mutase and human red blood cell 2,3-bisphosphoglycerate mutase including very similar active site sequences with a histidyl residue being involved in phospho group transfer. the liver bifunctional enzyme was found to catalyze the hydrolysis of glycerate 1,3-bisphosphate to glycerate 3-phosphate and inorganic phosphate. the km ...	1987	2824507
noradrenergic mechanisms in age-related cognitive decline.	the alpha-2 agonist, clonidine, improved spatial working memory performance in 13/13 aged rhesus monkeys with documented memory impairments. the clonidine response was blocked by alpha-2, but not alpha-1 antagonists, and appeared to result from actions at postsynaptic alpha-2 receptors in that area of cortex most critical for spatial working memory, the principal sulcal cortex. the data indicate that noradrenergic mechanisms play an important role in the functioning of the frontal association co ...	1987	2824692
replication and packaging of choleraphage phi 149 dna.	the intercellular replication of the circularly permuted dna of choleraphage phi 149 involves a concatemeric dna structure with a size equivalent to six genome lengths. the synthesis of both monomeric and concatemeric dnas during replication of phi 149 occurred in the cytoplasm. the concatemers served as the substrate for the synthesis of mature phage dna, which was eventually packaged by a headful mechanism starting from a unique pac site in the concatemeric dna. packaging of dna into phage hea ...	1987	2824829
[bacteriologic studies of bicarbonate dialysate and suitable initial solutions].	dialysates for the haemodialysis are produced unsterile and usually contain bacteria. own investigations of bicarbonate dialysate and adequate initial solutions comprised sterility tests, determinations of the germ count and germ tolerance experiments. only the "acid concentrate" was sterile. in the other solutions corynebacteria, acinetobacter and pseudomonas bacteria dominated as typical water germs. in the fresh reverse osmosis water and the bicarbonate dialysate as well as in the recently pr ...	1987	2825447
opioid receptors inhibit the adenylate cyclase in guinea pig cochleas.	the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ...	1987	2825909
opioid receptors inhibit the adenylate cyclase in guinea pig cochleas.	the effects of mu- and delta-preferring agonists on adenylate cyclase activity have been investigated in vitro in homogenates of guinea pig cochleas. morphine, leu-enkephalin, d-ala2, n-methyl-phe4, gly-ol5-enkephalin (dago) and d-ser2-leu-enkephalin-thr (dslet) each inhibited the synthesis of cyclic amp. this effect was reversed by naloxone which had a greater affinity in blocking the effect of the mu-preferring agonists (morphine, dago) than in blocking the effect of the delta-preferring agoni ...	1987	2825909
chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex.	sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors.	1987	2825912
chronic administration of sertraline, a selective serotonin uptake inhibitor, decreased the density of beta-adrenergic receptors in rat frontoparietal cortex.	sertraline, a potent and selective inhibitor of serotonin uptake, was chronically administered to laboratory rats. using in vitro receptor autoradiographic techniques, we found that the binding of [3h]dihydroalprenolol ([3h]dha) was reduced in cortex layers iv-vi. results of a saturation experiment indicated that the reduction in cortex layer iv was due to a change in number but not affinity of beta-adrenergic receptors.	1987	2825912
effects of caffeine and cyclic adenosine 3',5'-monophosphate on adenosine triphosphate-dependent calcium uptake by lysed brain synaptosomes.	the adenosine triphosphate-dependent calcium uptake by endoplasmic reticulum elements of lysed synaptosomes from rat brain cortex was studied. caffeine exhibited a biphasic effect on this calcium uptake activity: concentrations of 1, 2, 5, 10 or 30 mm caffeine stimulated calcium uptake by 62, 111, 73, 88 and 60% respectively, whereas calcium uptake was inhibited by 55% at a 60-mm concentration of caffeine. calcium release from endoplasmic reticulum elements of lysed brain synaptosomes was stimul ...	1987	2825917
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
dopamine denervation of the nucleus accumbens induces a selective increase in the number of delta-opioid binding sites.	selective lesions of dopamine (da) neurons of the nucleus accumbens were made in rats by local injections of 6-hydroxydopamine (6-ohda). seven days after 6-ohda, the binding affinities and capacities at mu-, delta- and kappa-opioid binding sites were determined in the nucleus accumbens by selective labelling techniques. 6-ohda pretreatment caused a significant increase in the number of delta-opioid binding sites (+30%) while the number of mu-, kappa- and total sites was not modified. the affinit ...	1987	2825926
murine cytomegalovirus-pseudomonas synergistic infections: comparison of virulent and attenuated virus.	the synergistic interactions between pseudomonas aeruginosa and virulent or attenuated murine cytomegalovirus (mcmv) were compared in vivo. virulent mcmv challenge at a dose of 5 x 10(5) pfu/mouse intraperitoneally, followed by intranasal superinfection with 5 x 10(6) cfu/mouse of pseudomonas aeruginosa after 48 h resulted in greater than 80% mortality, apparently owing to a failure of pulmonary clearance mechanisms. single infections, or the use of attenuated mcmv in synergistic infections, did ...	1987	2825950
successful islet transplantation in bb rats without chronic immunosuppression.		1987	3152652
projections from the pretectothalamic border area to the somatosensory part of the claustrum: an autoradiographic study.	injections of tritiated amino acid into the pretectothalamic border area of cats resulted in the appearance of numerous silver grains in the ipsilateral claustrum. the accumulation of grains was especially abundant in the dorsomedial area of the central part of the claustrum. these results indicate the presence of substantial projection from the pretectothalamic border area to the claustrum. it may be assumed that these projections belong to the somatosensory system, since they connect mainly di ...	1987	3442271
the hemolytic activity of some strains belonging to genus vibrio.		1987	3329505
[trial use of embryonal and early-fetal thymus extracts in the treatment of non-healing skin defects. ii. crural ulcers in humans].		1987	3329747
imipenem/cilastatin for pediatric infections in hospitalized patients.	one hundred and four children who were hospitalized for documented or suspected non-cns bacterial infections (56 males/48 females, 22 days to 15 years old) were treated with intravenous imipenem/cilastatin for 9.4 days (range 3 to 28 days). children up to three years of age received 100 mg/kg/day and older children 60 mg/kg/day, administered in four divided doses. bacterial pathogens were isolated before therapy in 85%. diagnoses in the 74 evaluable patients included bronchopneumonia with or wit ...	1987	3331043
t cell factors involved in the isotype-specific regulation of the ige antibody response.		1987	3333779
effects of levamisole on experimental infections by plasmodium berghei in mice.		1987	3333878
effects of levamisole on experimental infections by plasmodium berghei in mice.		1987	3333878
[synthesis and secretion of ceruloplasmin by isolated rat hepatocytes].	the synthesis and secretion of ceruloplasmin (cp) by isolated rat hepatocytes were investigated. cp released by liver cells appeared to have properties similar to those of the blood-circulating protein, i. e. mr, oxidase activity, immunological specificity and the peptide set of tryptic fingerprints. the polypeptides with mr of 130,000, 65,000, 48,000 and 18,000 were revealed in cp isolated from the incubation medium. these results suggest the susceptibility of the single-chain protein molecule ...	1987	3427129
cytoplasmic ca2+ concentration of single normal human and bovine parathyroid cells measured by dual wavelength microfluorometry.	dual wavelength microfluorometry was utilized to measure the cytoplasmic calcium concentration (cai2+) of single parathyroid cells loaded with the indicator fura-2. the method enabled the first registrations of cai2+ of normal human parathyroid cells, available only in minute numbers. at 0.5 mm extracellular ca2+, the cai2+ levels were similar in normal human and bovine cells. both cell types responded with an initial cai2+ transient followed by a sustained increase when raising extracellular ca ...	1987	3427219
tumor therapy of neoplastic diseases with tumor cells and neuraminidase: experimental studies on chessboard vaccination in transplantation tumors.	the therapeutic effect of intradermal (i.d.) injection of tumor cells mixed with vcn on growth of spontaneous metastases in transplantable tumors in mice (3-lewis lung adenocarcinoma; b16-melanoma) and rats (r-3230 mammary adenocarcinoma) was investigated. intradermal injection was done in a chessboard-like manner; increasing numbers (10(5), 10(6) and 10(7)) of mitomycin-treated tumor cells (m-tc) were each mixed with increasing amounts (10, 50 and 100 mu) of vibrio cholerae neuraminidase (vcn). ...	1987	3429076
large production of cholera toxin by vibrio cholerae o1 in yeast extract peptone water.	a large amount of cholera toxin (ct) was produced by vibrio cholerae o1 cultured in yeast extract-peptone water. the organisms were cultured initially in a stationary test tube (small surface-to-volume ratio) until the end of the exponential phase and subsequently cultured in a shaking flask for 15 to 20 h. by this method (previously reported as the aki-sw method), most cholera vibrios produced an abundance of ct (up to 64 micrograms/ml), regardless of their biotype and serotype. a substantial a ...	1987	3429623
construction of donor strains of classical and el tor biotypes of vibrio cholerae using r-plasmids and genetic mapping of the v. cholerae chromosome.		1987	3429794
release of endotoxin by toxigenic and non-toxigenic vibrio cholerae 01.		1987	3429800
delivery of theophylline into excised human skin from alkanoic acid solutions: a "push-pull" mechanism.	human skin samples are permeable to theophylline delivered from 1.5% solutions in various alkanecarboxylic acids and their mixtures. the respective permeability coefficients of theophylline, calculated from steady-state flux, correlate negatively with the permeability coefficients of the donor carboxylic acids and positively with the excess free energy of theophylline in the donor phase, or "push" effect. an exception is propionic acid which enhances the penetration of theophylline by promoting ...	1987	3430340
sperm retention cysts in the efferent duct and testicular atrophy of te inbred rats.	sperm retention cysts were observed in the efferent duct of te inbred rats at 15 weeks of age. spermatozoa agglutinated in the efferent duct with destruction of the epithelium. testicular weight increased or decreased. diameter of the seminiferous tubules was enlarged in testes that increased in weight and germinal cells degenerated in the atrophied testes. after 30 weeks of age, the tubules of the efferent duct were sometimes replaced by scars and testicular atrophy became more frequent. in 3 o ...	1987	3430479
liver cell lines.		1987	3431441
vibriocidal titre in cholera cases and contacts: its value in assessing endemicity of or susceptibility to cholera.	vibriocidal antibody titre in excess of 1:40 occurred within two weeks of cholera infection, both in severe hospitalized cases, contact cases and in asymptomatic infected contacts. these levels, considered to be indicative of protection, persisted for six months or longer in more than half of the subjects irrespective of presence and severity of symptoms. approximately 40% of infected family contacts had similar titres implying recent infection and subsequent protection. the use of antibiotics t ...	1987	3433343
[systemic immunity of experimental animals immunized with cholera vaccines].	the levels of antitoxic and vibriocidal antibodies in the sera of suckling rabbits after their parenteral immunization with cholera vaccine, cholera toxoid and a combination of cholera vaccine and toxoid were examined. cholera vaccine induces intensive production of vibriocidal antibodies, and cholera toxoid, of antitoxic antibodies. the parenteral administration of the serum of rabbits immunized with cholera toxoid neutralized the action of cholera toxin in the small intestine of suckling rabbi ...	1987	3434048
influence of sulphur dioxide breathing on defensive reflexes of the airways.	the influence of sulphur dioxide (so2) on cough and expiratory reflexes was studied in 22 anaesthetized (pentobarbital, 30 mg/kg i.v.) rabbits. the cough reflex (cr) was elicited by tracheobronchial mucous membrane stimulation, using a soft venous catheter. in order to induce the expiratory reflex (er) irritation of the larynx was performed by a silon fibre loop. the strength of both reflexes was assessed from the interpleural pressure fluctuation before so2 breathing, immediately after, and 30- ...	1987	3434302