Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| biochemical characterization of buffalo (bubalus bubalis) milk lysozyme. | 2003 | 14649419 | |
| polymorphisms in mhc-dra and -drb alleles of water buffalo (bubalus bubalis) reveal different features from cattle dr alleles. | seventy-five individuals of bubalus bubalis belonging to four different breeds, three of river buffalo and one of swamp buffalo, were studied for polymorphism in mhc drb (bubu-drb) and dra (bubu-dra) loci. eight alleles of bubu-drb were found, and all alleles in the swamp type were shared with the three river breeds. all alleles sampled from the breed of european origin (mediterranean) were present in breeds sampled in brazil, thus variability of this locus may have been preserved to a great ext ... | 2003 | 12580780 |
| innervation of vas deferens and accessory male genital glands in the water buffalo (bubalus bubalis). neurochemical characteristics and relationships to the reproductive activity. | autonomic nerves supplying mammalian male internal genital organs have an important role in the regulation of reproductive function. to find out the relationships between the neurochemical content of these nerves and the reproductive activity, we performed a histochemical and immunohistochemical study in a species, the water buffalo, exhibiting a seasonal sexual behaviour. the distribution of noradrenergic and nitric oxide synthase (nos)- and peptide-containing nerves was evaluated during the ma ... | 2003 | 12600736 |
| partial cloning and localization of leptin and leptin receptor in the mammary gland of the egyptian water buffalo. | originally an overall metabolic control was attributed to the leptin hormone, which is produced mainly by the adipose tissue. recently, leptin gene expression was demonstrated in several additional peripheral tissues. furthermore, several isoforms of leptin receptor were found both in the central nervous system and in the peripheral tissues. using reverse transcription and polymerase chain reaction analysis we demonstrate that leptin is expressed both in the adipose tissue and in the lactating m ... | 2003 | 14550514 |
| brazilian isolates of trypanosoma (megatrypanum) theileri: diagnosis and differentiation of isolates from cattle and water buffalo based on biological characteristics and randomly amplified dna sequences. | we detected and cultivated isolates of trypanosoma (megatrypanum) theileri from cattle and water buffaloes in são paulo state, southeastern brazil, which were characterized by comparing morphological, growth and molecular features. although isolates from cattle and water buffalo were morphologically indistinguishable, they differed in their growth characteristics. a dendrogram based on randomly amplified polymorphic dna (rapd) patterns indicated close-genetic relationships among all isolates fro ... | 2003 | 14559162 |
| clastogenic effects of the fasciolicide drug fasinex on river buffalo lymphocyte cultures in vitro. | fasinex (triclabendazole) has been reported to be an active fasciolocidal agent used in humans and in farm animals. the clastogenic effects of fasinex were tested in lymphocyte cultures of the river buffalo at three final concentrations: 25, 50 and 100 microg/ml. chromosomal aberrations, sister chromatid exchanges and micronucleus formation are the three cytogenetic parameters used in this study. the results demonstrated that the number of cells with different types of chromosomal aberrations, i ... | 2003 | 14568300 |
| demonstration of alternative and classical complement pathway activity in colostrum from buffalo (bubalus bubalis). | buffalo colostrum caused lysis of unsensitized red blood cells (rbc) from sheep, goats, rabbits and chickens. rbc from cattle and buffalo were resistant to lysis. that lysis was due to the presence of natural antibodies to these rbc was ruled out since there was no reduction in haemolytic titres even after adsorption with the respective rbc. the addition of egta to the diluent had no effect on the haemolytic activity. these findings indicate the presence of alternative complement pathway (acp) a ... | 2003 | 14582743 |
| response of anestrus rural buffaloes (bubalus bubalis) to intravaginal progesterone implant and pgf2alpha injection in summer. | an investigation was conducted to study the genital condition and response of intravaginal progesterone implant with and without administration of pgf2alpha in anestrus rural buffaloes during summer (april-june, 2001) in tropical climate. the ovarian status of 40 lactating (1st to 4th lactation) noncycling buffaloes and 15 noncycling buffalo heifers (3.5 to 5.0 years of age) maintained under village management condition were examined per rectum. inactive genitalia with [12] and without [28] palp ... | 2003 | 14610366 |
| sex determination of buffalo embryos (bubalus bubalis) by polymerase chain reaction. | the aim of this study was to identify a simple, rapid method for sex determination of in vitro produced buffalo embryos, amplifying y-chromosome-specific repeat sequences by polymerase chain reaction (pcr). buffalo oocytes collected from slaughtered animals were matured, fertilised and cultured in vitro for 7 days. on day 7 embryos were evaluated and divided in to six groups according to developmental stage (2, 4, 8, 16 cells, morulae and blastocyst). each embryo was stored singly in phosphate-b ... | 2003 | 12625525 |
| relationships between flavoring capabilities, bacterial composition, and geographical origin of natural whey cultures used for traditional water-buffalo mozzarella cheese manufacture. | natural whey cultures (nwc) (n = 29) used for traditional water-buffalo mozzarella cheese manufacture and arising from different geographical areas of production were characterized and grouped on the basis of their capability to develop neutral volatile compounds and according to their microbial diversity as revealed by molecular analysis. the flavoring properties of nwc were studied in dairy microcosms resembling the specific technological procedure used in the traditional water-buffalo mozzare ... | 2003 | 12647955 |
| mast cell and eosinophils in the wall of the gut and eosinophils in the blood stream during toxocara vitulorum infection of the water buffalo calves (bubalus bubalis). | toxocara vitulorum is a pathogenic nematode from the small intestine of very young buffalo calves. to understand the development of the inflammatory responses in the wall of the gut, samples of tissues were removed from the duodenum, jejunum and ileum of buffalo calves naturally infected with t. vitulorum during the beginning of the infection, at the peak of egg output, as well as during the periods of rejection of the worms and post-rejection. two additional control groups of uninfected calves ... | 2003 | 12651217 |
| black flies (diptera: simuliidae) attracted to humans and water buffalos and natural infections with filarial larvae, probably onchocerca sp., in northern thailand. | several simulium species were investigated as to their biting habits and natural infections with filarial larvae at ban pan fan, chiang mai province, in northern thailand. female adults flies landing on or flighting around a human and a water buffalo were collected during the daytime from 06.00 to 19.00 hours on 22 june 2001. as a result, 217 s. nodosum, 86 s. asakoae and two s. nigrogilvum were obtained from a human attractant, and 416 s. nodosum, 25 s. nakhonense, 16 s. asakoae, four s. fenest ... | 2003 | 12669343 |
| evaluation of brucellosis rb51 vaccine for domestic water buffalo (bubalus bubalis) in trinidad. | thirty-two young domestic water buffalo (bubalus bubalis) were obtained from a brucellosis-free farm to determine effectiveness of rb51 vaccination for prevention of brucella infection under natural-exposure conditions in trinidad. study animals (20 males and 12 females 5-20 months old) were assigned to vaccination or control groups, using a block randomization design ensuring equal sex distributions between groups. the vaccination group received commercially available rb51 at the recommended ca ... | 2003 | 12706059 |
| effects of the water-miscible organic solvents on lactoperoxidase purified from creek-water buffalo milk. | water buffalo lactoperoxidase (wblpo) was purified with amberlite cg-50 (nh(4)(+) form) resin, cm-sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel-filtration chromatography from skimmed buffalo milk. the purity of the wblpo was shown with sds-page. the r(z) (a(412)/a(280)) value for the wblpo was 0.9. the optimum ph for the wblpo was at 6.0. the k(m) value at optimum ph and 25 degrees c was 0.13 mm. the v(max) value at optimum ph and 25 degrees c was 5.3 micro mol/min per ml. th ... | 2003 | 12733975 |
| ovarian follicular dynamics in buffalo cows (bubalus bubalis). | follicular growth in egyptian buffalo cows was monitored using genital tracts from 200 buffalo cows collected immediately after slaughter. according to the morphological appearance of the corpus luteum (cl), the corresponding oestrous cycle was divided into four stages: a (days 1-4), b (days 5-10), c (days 11-17) and d (days 18-21). within these stages the follicular population on the ovaries was evaluated and the dominant follicle (df) determined in all recovered ovaries. the functional status ... | 2003 | 12753556 |
| experimental sarcocystis hominis infection in a water buffalo (bubalus bubalis). | a water buffalo (bubalus bubalis) was fed 5.0 x 10(5) sarcocystis hominis sporocysts from a human volunteer who had ingested s. hominis cysts from naturally infected cattle. a necropsy was performed on the buffalo 119 days after inoculation, and a large number of microscopic sarcocysts (approximately 5,000/g) were found in skeletal muscles. ultrastructurally, the sarcocyst wall from buffalo muscles has upright villar protrusions measuring about 5.6 x 0.8 microm with numerous microtubules that ru ... | 2003 | 12760663 |
| hormonal dynamics and follicular turnover in prepuberal mediterranean italian buffaloes (bubalus bubalis). | the aim of this study was the investigation of hormonal and ovarian follicular dynamics in prepuberal buffaloes (bubalus bubalis) bred in italy. eleven 5-9-month old buffalo calves ranging in weight from 122 to 270kg, maintained under controlled nutritional and environmental conditions, underwent 50 days of ultrasonographic ovarian follicular monitoring in the months of october-december. blood sampling for e(2) and fsh determination and ultrasonographic monitoring using a 7.5mhz linear probe and ... | 2003 | 12763162 |
| purification of glucose 6-phosphate dehydrogenase from buffalo (bubalus bubalis) erythrocytes and investigation of some kinetic properties. | glucose 6-phosphate dehydrogenase (g6pd) was purified from buffalo (bubalus bubalis) erythrocytes and some characteristics of the enzyme were investigated. the purification procedure was composed of two steps: hemolysate preparation and 2('),5(')-adp-sepharose 4b affinity gel chromatography. thanks to the two consecutive procedures, the enzyme, having a specific activity of 69.7eu/mg proteins, was purified 650-fold with a yield of 31%. optimal ph, stable ph, optimal temperature, molecular weight ... | 2003 | 12767823 |
| the autonomous innervation of the buffalo (bubalus bubalis) testis. an immunohistochemical study. | the innervation pattern in the buffalo testis was determined by using histochemical and immunohistochemical methods. nerves were concentrated in the tunica albuginea and septula testis, and did not show an uniform distribution. the tunica albuginea at the lateral and medial sides and at the free border of the testis is most densely innervated than at the epididymal border. at the cranial pole thick nerve bundles were observed between albugineal vessels and muscle bundles. rare parenchymal nerves ... | 2003 | 12777213 |
| immunohistochemical distribution of s-100 protein and subunits (s100-alpha and s100-beta) in the swamp-type water buffalo (bubalus bubalis) testis. | the distribution and localization of s-100 protein (s-100) and its subunits (s100-alpha and s100-beta) in the testis of swamp-type water buffalo were investigated using immunohistochemistry. s-100 was detected in the sertoli cells in the convoluted seminiferous tubules, modified sertoli cells lining the terminal segment of the seminiferous tubules and in the intratesticular excurrent ducts (straight tubules and rete testis). s100-beta showed the same distribution and localization with that of s- ... | 2003 | 12780530 |
| immunohistochemical evaluation of the muscularis mucosae in the ruminant forestomach. | the muscularis mucosae and condensed fibrous layer of the ruminant forestomach were studied by immunohistochemistry using specific antibodies against alpha-smooth muscle actin (alphasma) and gamma-smooth muscle actin (gammasma). the specimens were collected from the rumen, reticulum and omasum of cattle, water buffalo, sheep, goat, barbary sheep, japanese serow, sika deer and mouse deer. the muscularis mucosae showed immunoreactivity for both alphasma and gammasma. on the other hand, the condens ... | 2003 | 12823104 |
| sperm surface hyaluronan binding protein (habp1) interacts with zona pellucida of water buffalo (bubalus bubalis) through its clustered mannose residues. | sperm-oocyte interaction during fertilization is multiphasic, with multicomponent events, taking place between zona pellucida (zp) glycoproteins and sperm surface receptor. d-mannosylated glycoproteins, the major constituents of zp are considered to serve as ligands for sperm binding. the presence of hyaluronan binding protein 1 (habp1) on sperm surface of different mammals including cattle and its possible involvement in sperm function is already reported. recently, we have demonstrated the spe ... | 2003 | 12506357 |
| developmental failure of hybrid embryos generated by in vitro fertilization of water buffalo (bubalus bubalis) oocyte with bovine spermatozoa. | the developmental potential of inter-species hybrid embryos produced by in vitro fertilization of in vitro matured buffalo oocytes with bovine spermatozoa was studied with a view to investigate pre-implantation embryo development and its gross morphology, early embryonic gene expression, and embryonic genome activation. fertilization events with both buffalo and cattle spermatozoa were almost similar. overall fertilization rate with cattle spermatozoa was 78.4% was not significantly different fr ... | 2003 | 12548668 |
| chromosome localization of the 31 type i texas bovine markers in sheep and goat chromosomes by comparative fish-mapping and r-banding. | bovine bac clones containing the 31 genes, referred to as the texas markers used earlier to definitively assign the 31 bovine syntenic groups (u) to cattle chromosomes, were mapped by fluorescent in situ hybridization to sheep and goat r-banded chromosomes according to iscndb2000. all 31 markers were localized on homoeologous chromosomes and chromosome bands of the two species in agreement with previous localizations obtained both in cattle and river buffalo, definitively confirming chromosome h ... | 2003 | 12873218 |
| microvascular architecture of the fetal cotyledons in water buffaloes (bubalus bubalis) during different stages of pregnancy. | to elucidate the morphological background of physiological differences between bovine and buffalo gestation forty-two placentae ranging from the 3rd to 10th month of pregnancy were used to study the microvascular architecture of the fetal cotyledons in the buffalo. the tissues were prepared for light and scanning electron microscopy by paraformaldehyde fixation and corrosion casting of the fetal cotyledonary vascular system. histology and vascular casts revealed the buffalo fetal cotyledons to c ... | 2003 | 12924470 |
| glutathione synthesis during in vitro maturation of buffalo (bubalus bubalis) oocytes: effects of cysteamine on embryo development. | it was demonstrated that cysteamine supplementation during in vitro maturation (ivm) improves embryo development by increasing glutathione (gsh) synthesis in several species. an improved developmental competence of oocytes matured in the presence of cysteamine was also recorded in buffalo species. the purpose of this work was to investigate (1) if glutathione is de novo synthesized during in vitro maturation of buffalo oocytes, (2) if cysteamine improves buffalo embryo development via an increas ... | 2003 | 12935871 |
| detection of water buffalo sex chromosomes in spermatozoa by fluorescence in situ hybridization. | in order to identify x- and y-bearing spermatozoa in water buffalo by fluorescence in situ hybridization (fish), some available probes of closely related species were examined. an x- and y-specific probe set, made from flow sorted yak chromosomes, labelled in somatic metaphases of water buffalo the whole x and y, respectively, except their centromere regions. a cattle y-chromosome repeat sequence (bc1.2) showed strong signal on the telomere region of the buffalo y-chromosome, demonstrating the e ... | 2003 | 12950688 |
| buffalo and cattle hybrid embryo development is decreased by caffeine treatment during in vitro fertilization. | water buffalo are renowned for difficulties in the implementation of assisted reproductive technologies, with both males and females being problematic. in this study, we used cattle oocytes to assess the effect of treatments with heparin and caffeine on buffalo spermatozoa and subsequent fertilization and embryo development in vitro. there was no significant difference between buffalo and bovine spermatozoa in the events associated with fertilization. fertilization of cattle oocytes with buffalo ... | 2003 | 12517375 |
| estimation of receiver-operating characteristic curves to determine accuracy of a competitive enzyme-linked immunosorbent assay for the serodiagnosis of brucella infection in domestic water buffalo (bubalus bubalis) and cattle. | to estimate receiver-operating characteristic (roc) curves for a competitive elisa (c-elisa) that is used in serodiagnosis of brucellosis in water buffalo and cattle, to determine the most appropriate positive cutoff value for the c-elisa in confirmation of infection, and to evaluate species differences in c-elisa function. | 2003 | 12518879 |
| bovine and buffalo in vitro embryo production using oocytes derived from abattoir ovaries or collected by transvaginal follicle aspiration. | this study was undertaken in order to evaluate the effect of oocyte source (live animals and abattoir ovaries) on subsequent embryo development in buffalo (bubalus bubalis). cow ovaries were also collected as oocyte donors for in vitro embryo production (ivep). three hundred thirty-eight oocytes were recovered by ovum pick up (opu, group a) from 8 pluriparous buffalo cows, while 1127 and 1457 oocytes were aspirated, respectively, from buffalo (group b) and bovine (group c) slaughterhouse ovaries ... | 2003 | 12527061 |
| estrus induction following pgf2alpha treatment in the superovulated buffalo (bubalus bubalis). | early return-to-estrus after embryo collection would shorten the interval between consecutive superovulations and improve efficiency of embryo production. following superovulation and embryo collection, 80 buffaloes were treated with 15.0mg luprostiol (pgf2alpha analogue) for the induction of luteolysis and early return-to-estrus. a total of 67.5% donor animals returned to estrus, on average 11.8+/-0.84 days after the pgf2alpha treatment. the number of ovulations (<or=5 or >5 cl) had no signific ... | 2003 | 12527068 |
| cross-cultural management of pest animal damage: a case study of feral buffalo control in australia's kakadu national park. | government agencies responsible for pest animal management often assume that their views and assumptions about the benefits of control are widely shared, especially if these pests are exotics. this was certainly the case when tens of thousands of feral asian water buffalo (bubalus bubalis) were to be culled in australia's kakadu national park as part of a national brucellosis and tuberculosis eradication campaign (btec). implementation of the campaign sparked considerable dispute between officia ... | 2003 | 14986894 |
| chromosomal localization of sixty autosomal loci in sheep (ovis aries, 2n = 54) by fluorescence in situ hybridization and r-banding. | sixty autosomal loci (5 type i and 55 type ii) from 24 bovine syntenic groups, and previously fish-mapped to goat and river buffalo chromosomes, were localized by fluorescence in situ on sheep (ovis aries, 2n = 54) chromosomes, thereby notably extending the cytogenetic map of this economically important species. caprine bac clones were hybridized to r-banded chromosome preparations. fitc-signals and rbpi- banding (r-banding by late brdu-incorporation and propidium iodide staining) were simultane ... | 2003 | 15004476 |
| assignment of solute carrier family 26 (sulfate transporter), member 2 (slc26a2) to river buffalo (bubalus bubalis, 2n = 50) chromosome 9q26 (bbu9q26) by fluorescence in situ hybridization and r-banding. | 2003 | 15008140 | |
| a pcr-based rflp analysis of sarcocystis cruzi (protozoa: sarcocystidae) in yunnan province, pr china, reveals the water buffalo (bubalus bubalis) as a natural intermediate host. | a polymerase chain reaction-based restriction fragment length polymorphism (rflp) approach is used to examine sarcocystis cruzi-like taxa from the atypical intermediate host, water buffalo, in yunnan, people's republic of china. the loci examined lie within the 18s rrna gene. a total of 15 water buffalo isolates are compared with those of 10 s. cruzi from cattle. rflp patterns for the s. cruzi isolates from cattle and the s. cruzi-like taxon from water buffalo are found to be identical with all ... | 2002 | 12537125 |
| expression of rotat 1.2 cross-reactive variable antigen type in trypanosoma evansi and t. equiperdum. | the variable antigen type (vat) rotat 1.2 has been cloned from a t. evansi strain, isolated in 1982 from a water buffalo in indonesia. all t. evansi isolates hitherto tested express this vat. in a study on the differential diagnosis of t. equiperdum and t. evansi in horses, we investigated serological evidence for the expression of rotat 1.2 in 11 t. evansi and six t. equiperdum populations originating from asia, europe, africa, and the americas. preinfection sera and sera of days 7, 14, 25, and ... | 2002 | 12381586 |
| field testing of schistosoma japonicum dna vaccines in cattle in china. | vaccines are needed to reduce the zoonotic reservoir of schistosoma japonicum infection in bovines in china. we have developed two experimental dna vaccines and have already shown these to be capable of inducing partial protection in water buffalo naturally exposed to the risk of s. japonicum infection in the field. we now report a similar field trial in cattle, the other major bovine reservoir host species in china. groups of cattle were vaccinated with the vrsj28 vaccine or the vrsj23 vaccine, ... | 2002 | 12399187 |
| comparison of serologic tests for detection of brucella infections in cattle and water buffalo (bubalus bubalis). | to estimate sensitivity and specificity of 4 commonly used brucellosis screening tests in cattle and domestic water buffalo of trinidad, and to compare test parameter estimates between cattle and water buffalo. | 2002 | 12428673 |
| folliculogenesis in buffalo (bubalus bubalis): a review. | the urgent need for improving the reproductive performance of buffalo necessitates a better understanding of the mechanisms controlling ovarian follicular growth and development. attention needs to be focused on improving superovulation responses and conception rates, and reducing the variability in ovulation rate and embryo loss. application of ultrasonic imaging has revealed that follicular turnover during an unstimulated oestrous cycle occurs in waves, with each wave involving synchronous dev ... | 2002 | 12467356 |
| control of ovulation with a gnrh agonist after superstimulation of follicular growth in buffalo: fertilization and embryo recovery. | the potential to use a gnrh agonist bioimplant and injection of exogenous lh to control the time of ovulation in a multiple ovulation and embryo transfer (moet) protocol was examined in buffalo. mixed-parity buffalo (bubalus bubalis; 4-15-year-old; 529 +/- 13 kg lw) were randomly assigned to one of five groups (n = 6): group 1, conventional moet protocol; group 2, conventional moet with 12 h delay in injection of pgf2alpha; group 3, implanted with gnrh agonist to block the preovulatory surge rel ... | 2002 | 12472135 |
| production of recombinant buffalo (bubalus bubalis) and goat (capra hircus) growth hormones from genetically modified e. coli strains. | the growth hormone cdnas of indian reverine buffalo (bubalus bubalis) and beetal goat (capra hircus) were cloned in escherichia coli through rt-pcr technique. nucleotide sequencing revealed several silent mutations in both cdnas and only one amino acid change in the case of goat when compared to reported bovine (bos taurus) sequence. the high level expression of both the polypeptide hormones was achieved in e. coli (> or =30% of soluble intracellular proteins) through the construction of two-cis ... | 2002 | 12084476 |
| buffalo (bubalus bubalis) interleukin-2: sequence analysis reveals high nucleotide and amino acid identity with interleukin-2 of cattle and other ruminants. | a 4400-bp genomic sequence and a 332-bp truncated cdna sequence of the interleukin-2 (il-2) gene of indian water buffalo (bubalus bubalis) were amplified by polymerase chain reaction and cloned. the coding sequence of the buffalo il-2 gene was assembled from the 5' end of the genomic clone and the truncated cdna clone. this sequence had 98.5% nucleotide identity and 98% amino acid identity with cattle il-2. three amino acid substitutions were observed at positions 63, 124 and 135. comparison of ... | 2002 | 12121282 |
| mitochondrial 12s rdna sequence relationships suggest that the enigmatic bovid "linh duong" pseudonovibos spiralis is closely related to buffalo. | the phylogenetic position and taxonomic status of the recently described southeast asian endemic bovid pseudonovibos spiralis were deduced from nearly complete 12s mitochondrial rdna sequences of this species and bubalus bubalis alongside 26 sequences of bovidae from genbank using cervus elaphus (cervidae) as outgroup. maximum-likelihood analyses performed by puzzle and fastdnaml nested p. spiralis at the base of the subtribe buffalo bovini, suggesting the close relationship of this enigmatic sp ... | 2002 | 12182406 |
| residue study of ivermectin in plasma, milk, and mozzarella cheese following subcutaneous administration to buffalo (bubalus bubalis). | the distribution of ivermectin in buffalo plasma and milk after administration of a single subcutaneous dose (0.2 mg kg(-)(1) b.w.) was studied. ivermectin reached the maximal concentration in plasma (28.5 +/- 1.7 ng ml(-)(1)) and milk (23.6 +/- 2.6 ng ml(-)(1)) after 2.4 +/- 0.32 and 2.8 +/- 0.44 days, respectively. the drug showed a parallel disposition in milk and plasma, with a ratio of 1.12 +/- 0.16. ivermectin concentrations were detected in mozzarella cheese obtained from milk collected o ... | 2002 | 12188637 |
| apoptosis during spontaneous and prostaglandin f(2alpha)-induced luteal regression in the buffalo cow (bubalus bubalis): involvement of mitogen-activated protein kinases. | the present study was conducted to evaluate whether the corpus luteum (cl) of the water buffalo (bubalus bubalis) cow undergoes luteal regression by the process of apoptosis and to examine the involvement of mitogen-activated protein (map) kinases during prostaglandin (pg) f(2alpha)-induced luteolysis. sections of cl from late in the estrous cycle, i.e., during spontaneous luteolysis, stained for 4',6'-diamidino-2-phenylindole revealed increased numbers of condensed nuclei, indicating cell death ... | 2002 | 12193381 |
| efficacy of pgf(2alpha) to synchronize estrus in water buffalo cows (bubalus bubalis) is dependent upon plasma progesterone concentration, corpus luteum size and ovarian follicular status before treatment. | this study was conducted to identify factors affecting pgf(2alpha) efficacy to synchronize estrus in water buffalo cows. after detection of a corpus luteum (cl) by rectal palpation, cows were treated (im) with dinoprost (12.5, 25 or 50mg) or d(+) cloprostenol (75, 150 or 300 microg) in a total of 66 treatments. blood samples were collected 0, 24 and 48 h after treatment and ultrasound examinations and observations for estrus were performed daily to the day of ovulation or to 6 days after treatme ... | 2002 | 12220816 |
| identification of adulteration in water buffalo mozzarella and in ewe cheese by using whey proteins as biomarkers and matrix-assisted laser desorption/ionization mass spectrometry. | a rapid and accurate method to identify bovine and ewe milk adulteration of fresh water buffalo mozzarella cheese by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms) is described. the differentiation among mozzarella made from water buffalo milk and from mixtures of less expensive bovine and, more recently, ewe milk with water buffalo milk is achieved using whey proteins, alpha-lactalbumin and beta-lactoglobulins as molecular markers. it is worth n ... | 2002 | 12271441 |
| phylogeny of bovine species based on aflp fingerprinting. | the bovini species comprise both domestic and wild cattle species. published phylogenies of this tribe based on mitochondrial dna contain anomalies, while nuclear sequences show only low variation. we have used amplified fragment length polymorphism (aflp) fingerprinting in order to detect variation in loci distributed over the nuclear genome. computer-assisted scoring of electrophoretic fingerprinting patterns yielded 361 markers, which provided sufficient redundancy to suppress stochastic effe ... | 2002 | 11813106 |
| transvaginal ultrasound-guided aspiration of follicles from indian buffaloes (bubalus bubalis) with reproductive problems. | 2002 | 11817861 | |
| some comparative aspects of the pharmacokinetics of tylosin in buffaloes and cattle. | the pharmacokinetics of tylosin were compared in cattle (bos taurus) and buffaloes (bubalus bubalis). six animals received each a single dose of 10 mg/kg of tylosin tartrate by the intramuscular route. the serum concentration (cmax) and the volume of distribution (vd) presented significant differences between the two species. cmax was 0.40 +/- 0.046 microg/ml for buffaloes and 0.64 +/- 0.068 microg/ml for cattle. vd was 1.91 +/- 0.12 l/kg and 1.33 +/- 0.09 l/kg for buffaloes and cattle, respecti ... | 2002 | 11860087 |
| production of buffalo (bubalus bubalis) embryos in vitro: premises and promises. | techniques for in vitro production (ivp) of buffalo embryos adopting the procedures developed in cattle have received increasing interest in the recent times. a high oocyte maturation, fertilization and cleavage rate and a low rate of blastocyst yield and calving following transfer of in vitro produced buffalo embryos have been obtained. the efficiency of ivp in buffalo is much lower than that in cattle. several problems need to be resolved before ivp technology can be used regularly in buffalo ... | 2002 | 11975742 |
| rumen ciliate faunae of water buffalo (bubalus bubalis) and goat (capra hircus) in nepal. | rumen ciliate composition of river-type water buffalo and goat in nepal was surveyed. as the result of survey, 13 genera representing 52 species and 20 formae of the ciliates were identified. of them 13 genera with 44 species and 9 formae were found from the water buffalo and 8 genera with 21 species and 12 formae from the goat. the present paper shows the first report of hsiungella triciliata, entodinium brevispinum, e. convexum, e. javanicum, e. rectangulatum f. rectangulatum, e. rectangulatum ... | 2002 | 11999448 |
| mhc-drb exon 2 allele polymorphism in indian river buffalo (bubalus bubalis). | the polymorphism of the major histocompatibility complex (mhc) class ii drb gene of riverine buffalo (bubalus bubalis) was studied. second exon sequences from the buffalo drb locus, homologous to the cattle drb3 gene, were amplified and characterized. a combination of single strand conformation polymorphism (sscp) and heteroduplex analysis (ha) in a non-denaturing gel was used to identify new drb second exon sequences. sscp, ha and finally sequencing allowed the identification of 22 mhc-drb exon ... | 2002 | 12030926 |
| xenonuclear transplantation of buffalo (bubalus bubalis) fetal and adult somatic cell nuclei into bovine (bos indicus) oocyte cytoplasm and their subsequent development. | bovine oocyte cytoplasm has been shown to support the development of nuclei from other species up to the blastocyst stage. somatic cell nuclei from buffalo fetal fibroblasts have been successfully reprogrammed after transfer to enucleated bovine oocytes, resulting in the production of cloned buffalo blastocysts. the aim of this study was to compare the in vitro development of fetal and adult buffalo cloned embryos after the fusion of a buffalo fetal fibroblast, cumulus or oviductal cell with bov ... | 2002 | 12041687 |
| in vitro culture of buffalo (bubalus bubalis) preantral follicles. | growth of buffalo preantral follicles in culture was studied to investigate the effect of size of preantral follicles, individual or group culture, long-term culture of preantral follicles for (40 days), addition of human follicle stimulating hormone (fsh), insulin-transferrin-selenium (its), growth factors (epidermal growth factor (egf), fibroblast growth factor (fgf), vaso active intestinal polypeptide (vip) in culture media, and substitution of pregnant mare serum gonadotrophin (pmsg) for fsh ... | 2002 | 12041688 |
| hormonal stimulation and oocyte maturational competence in prepuberal mediterranean italian buffaloes (bubalus bubalis). | the objective of this study was to determine the best combined hormonal treatment to utilize in order to obtain a high number of good quality in vivo and in vitro matured oocytes from prepuberal mediterranean italian buffalo calves (bubalus bubalis). transvaginal ultrasound follicular aspiration was employed to recover oocytes from antral follicles. fifteen barn housed buffalo calves, between 5 and 9 months of age were used in this study and randomly divided into control (group a) and treated gr ... | 2002 | 12041691 |
| ancient specimens and dna contamination: a case study from the 12s rrna gene sequence of the "linh duong" bovid (pseudonovibos spiralis). | in 1993, several unusual horn sheaths, collected in the south of vietnam, were regarded as evidence for a new mammal species, pseudonovibos spiralis. however, the taxonomic status of p. spiralis remains a highly controversial subject: firstly, it has been related to three different groups of the family bovidae: antilopini (gazelles), bovini (oxen, bisons, and buffaloes), and caprini sensu lato (goats, sheep and allies). secondly, certain horns have been shown to be faked. most recently, it has b ... | 2002 | 12046628 |
| a set of cattle microsatellite dna markers for genome analysis of riverine buffalo (bubalus bubalis). | one hundred and eight microsatellite primer pairs, originally identified from cattle, were evaluated for their applicability in buffalo. eighty-one primer pairs (75%) amplified discrete products, and of these, 61 pairs (56%) gave polymorphic band patterns on a panel of 25 buffaloes. the mean number of alleles per polymorphic marker was 4.50 +/- 0.20, and the mean heterozygosity per polymorphic marker was 0.66 +/- 0.02. successful genotyping of buffaloes using cattle specific primers suggests tha ... | 2002 | 12047229 |
| comparison of two ovsynch protocols (gnrh versus lh) for fixed timed insemination in buffalo (bubalus bubalis). | we evaluated the efficiency of replacing gnrh with lh in the ovulation synchronization protocol in buffaloes. buffaloes received gnrh on day 0, (buserelin; conceptal, 20 microg), pgf2alpha (luprostiol; prosolvin, 15 mg) on day 7 and gnrh (buserelin; conceptal, 10 microg; group 1) or porcine lh (lh; lutropin-v, 12.5 mg; group 2) on day 9. in experiment 1, we studied the follicular dynamics of 30 buffaloes (group 1, n = 15 and group 2, n = 15). we performed ultrasonography every 12 h from days 0 t ... | 2002 | 12054201 |
| in vitro production of cattle-water buffalo (bos taurus--bubalus bubalis) hybrid embryos. | interspecific hybrid embryos are useful models for the study of maternal-fetal interactions, transmission pattern of species-specific markers and parental contributions to growth and developmental potential of pre-attachment embryos. in an attempt to investigate the possibility of producing hybrid embryos of domestic cattle (bos taurus) and water buffalo (bubalus bubalis), cattle oocytes were exposed to buffalo sperm and buffalo oocytes were exposed to cattle sperm and the cleavage rate and the ... | 2002 | 12056456 |
| purification of lactoperoxidase from creek-water buffalo milk and investigation of kinetic and antibacterial properties. | water buffalo lactoperoxidase (wblp) was purified with amberlite cg 50 h+ resin, cm sephadex c-50 ion-exchange chromatography, and sephadex g-100 gel filtration chromatography from skim milk. all purification steps of the wblp were shown with sds-page and rz (a412/a280) controlled the purification degree of the enzyme. rz value for the purified wblp was 0.8. to determine purification steps and kinetic properties, the activity of enzyme was measured by using 2,2-azino-bis-(3-ethylbenzthiazoline-6 ... | 2002 | 12071645 |
| purification and partial characterization of bacillocin 490, a novel bacteriocin produced by a thermophilic strain of bacillus licheniformis. | background: applications of bacteriocins as food preservatives have been so far limited, principally because of their low antimicrobial activity in foods. nisin is the only bacteriocin of significant use, but applications are restricted principally because of its very low activity at neutral or alkaline ph. thus the isolation of new bacteriocins active in foods is desirable. results: we isolated a bacillus licheniformis thermophilic strain producing a bacteriocin with some novel features, named ... | 2002 | 12076356 |
| microvasculature of the buffalo epididymis. | the microvasculature of the water buffalo (bubalus bubalis) epididymis was investigated using light (lm), scanning electron (sem), and transmission electron (tem) microscopy techniques. sem analysis of the buffalo epididymis showed fenestrations that occupied ovoid inside the endothelium of the postcapillary venules located in the caput, corpus, and cauda. they varied in shape and dimension, but more importantly, they connected the venules of the blood vascular system to the capillaries of the p ... | 2002 | 11748572 |
| ixodid ticks on domestic animals in samut prakan province, thailand. | the prevalence of tick-harboring domestic animals, tick density, and the species of ticks were studied throughout the year 2000, in muang samut prakan, bang phli and phra pradaeng districts of samut prakan province. the animals examined were canis lupus familiaris (450), bos indicus (cross-bred) (189), bos taurus (30), bubalus bubalis (171) and sus scrofa domestica (450). the total number of collected ticks was 1,491. the pigs did not harbor ticks. the stages of ticks collected were larvae, nymp ... | 2002 | 12971472 |
| cloning, characterization, and expression studies in escherichia coli of growth hormone cdnas from indian zebu cattle, reverine buffalo, and beetal goat. | the growth hormone cdnas from three different economically important animal species of indian origin viz., indian zebu cattle (bos indicus), indian reverine buffalo (bubalus bubalis), and beetal goat (capra hircus) were isolated by the rt-pcr technique. the amplified product was then cloned into phagemid pbluescriptiiks- and the nucleotide sequence of the entire 573 base coding region for each product was determined. the genetic sequences as well as the translated protein sequence of these rumin ... | 2002 | 12517072 |
| comparative mapping of twenty-eight bovine loci in sheep (ovis aries, 2n = 54) and river buffalo (bubalus bubalis, 2n = 50) by fish. | peripheral blood cell cultures were treated for late incorporation of both brdu and hoechst-33258 to obtain r-banding pattern preparations. twenty-eight bovine cosmids from 19 bovine syntenic groups (u), three of which contain type i loci and 25 which contain microsatellite loci and have previously been assigned to cattle chromosomes, were comparatively fish-mapped to sheep and river buffalo chromosomes according to the standard karyotypes (13 loci for the first time in the latter species). the ... | 2002 | 12826750 |
| characterization of sarcocystis species in domestic animals using a pcr-rflp analysis of variation in the 18s rrna gene: a cost-effective and simple technique for routine species identification. | thirteen restriction endonucleases were used to investigate nuclotide sequence variation in the 18s rrna dna of 88 individuals from ten sarcocystis taxa collected as cysts from their intermediate hosts, swine, cattle and water buffalo. a dna sequence of approximately 900 bp was used. a total of 26 electromorphs were detected. the electromorphs were sorted into seven different haplotypes that coincided with the six named species and an unidentified species from cattle. these findings support thos ... | 2002 | 12856319 |
| histomorphometrical and comparative analysis of three muscles of buffalo (bubalus bubalis l.). | three muscles were analyzed, longissimus dorsi, semimembranosus and caput longum tricipitis brachii taken from nine cow buffaloes, by examining the histochemical and morphometrical characteristics of different muscle fibres types and their distribution inside the examined muscles. cross sectional area, perimeter, maximum and minimum diameter of about 200 fibres were measured for each muscle, and fast-twitch glycolytic fibres (fg), fast-twitch oxidative-glycolytic fibres (fog), slow-twitch oxidat ... | 2002 | 12611475 |
| onset and sequence of rba-band replication on the inactive x-chromosomes of cattle (bos taurus l.), river buffalo (bubalus bubalis l.) and goat (capra hircus l.). | this study was undertaken to provide cytogenetic information about onset and sequence of rba-band replication on the inactive x-chromosomes of cattle, river buffalo and goat. blood cultures were synchronized overnight with thymidine after 48 hours of growth. the cell block was released with fresh medium and the cells allowed to grow in the presence of brdu and h33258 for 1, 2, 4, 6, 8, 10, 12 and 14 hours, including 20 minutes colcemide. results show that: (a) the onset of rba-banding replicatio ... | 2002 | 12575792 |
| differentiation of cattle species in beef by pcr-rflp of mitochondrial and satellite dna. | methods currently used for the identification of the species origin of meat or tissue samples have not been validated for other bovine species than taurine cattle or water buffalo. these methods also do not discriminate between the different bovine species that are used as source of beef. here, we describe two complementary methods for detection and differentiation of bovine species, which are based on mutations in mitochondrial dna and centromeric satellite dna, respectively. the analysis of sa ... | 2002 | 22063639 |
| the use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (bubalus bubalis). | one of the major difficulties in studying the mechanisms of host defense in economically important species indigenous to asia and the middle east is the lack of monoclonal antibody (mab) reagents that define the immune systems of species other than cattle, goats, sheep, and pigs. one strategy that could obviate this problem at minimal cost is to identify existing mabs that recognize conserved epitopes on orthologous major histocompatibility complex (mhc) and leukocyte differentiation molecules. ... | 2001 | 14614279 |
| the disposition kinetics, urinary excretion and dosage regimen of ciprofloxacin in buffalo calves (bubalus bubalis). | the disposition kinetics, urinary excretion and a dosage regimen for ciprofloxacin after a single intravenous administration of 5 mg/kg was investigated in 5 healthy buffalo calves. the disposition kinetics were best fitted to a three-compartment open model. after 1 min, the concentration of ciprofloxacin in plasma was 8.50 +/- 0.39 microg/ml and the minimum therapeutic concentration was maintained for 10 h. the elimination half-life and volume of distribution were 3.88 and 0.08 h and 3.97 +/- 0 ... | 2001 | 11767010 |
| therapeutic efficacy of oxime reactivators in fenitrothion toxicity in buffalo calves (bubalus bubalis). | the therapeutic efficacy of 2-pyridine aldoxime methochloride and diacetylmonoxime (dam) alone and in combination with atropine was determined in oral fenitrothion toxicity in buffalo calves. dam alone and in combination with atropine constitute the most effective therapy of fenitrothion poisoning. as compared to 2-pyridine aldoxime methochloride, dam was also more effective in reactivating the fenitrothion-inhibited erythrocyte and plasma acetylcholinesterase and serum carboxylesterase enzymes ... | 2001 | 11402652 |
| the "linh duong" pseudonovibos spiralis (mammalia, artiodactyla) is a new buffalo. | the controversial phylogenetic position of the recently described south-east asian endemic bovid, pseudonovibos spiralis, was evaluated on the basis of phylogenetic analyses of originally obtained nearly complete 12s mitochondrial rdna sequences for this species and bubalus bubalis and 26 sequences of bovidae from the genbank using cervus elaphus (cervidae) as outgroup. in most of the phylogenetic analyses performed using paup 4.0 (maximum parsimony, maximum likelihood and neighbour-joining), bo ... | 2001 | 11402841 |
| effects of cooling and warming conditions on post-thawed motility and fertility of cryopreserved buffalo spermatozoa. | the principal objective of this study was to derive an improved procedure for cryopreservation of swamp buffalo (bubalus bubalis) spermatozoa. experiments were conducted to determine effects of cooling rate, intermediate plunge temperature and warming rate on motility and acrosome integrity of spermatozoa. spermatozoa were obtained from three bulls (three ejaculates/bull) and were subjected to nine cooling conditions before being frozen in liquid nitrogen: cooling at 10, 20, or 30 degrees c/min ... | 2001 | 11408115 |
| immune regulation of ovarian function in buffaloes (bubalus bubalus). | we studied the infiltration of different subsets of immune system cells in the ovarian parenchyma of egyptian buffaloes during follicular and luteal phases of the estrous cycle. all subsets of leukocytes infiltrated significantly more into corpora lutea (cl) than into graafian follicles (gf) (p < 0.01) except for plasma cells that were abundant in the gf but not observed in the cl. the number of macrophages, lymphocytes, neutrophils and eosinophils were significantly greater in mature cl than in ... | 2001 | 11233791 |
| effects of beta-carotene, selenium and vitamin a on in vitro polymorphonuclear leukocytic activity in peripartal buffalo (bubalus bubalus). | the effect of different concentrations of three antioxidans on phagocytic and kill activities of blood polymorphonuclear leukocytes (pmn) isolated from buffaloes during the peripartum period (4 weeks before to 7 weeks after parturition) was investigated in this study. two concentrations of beta-carotene and vitamin a (10(-6) and 10(-5) m) and one concentration of se (10(-9) m) were used. phagocytic activity of pmn treated with beta-carotene (10(-6)m) significantly enhanced (p < 0.05) after partu ... | 2001 | 11245259 |
| analysis of potential antigens of protoscoleces isolated from pulmonary and hepatic hydatid cysts of bubalus bubalis. | the aqueous soluble proteins of the protoscoleces of echinococcus granulosus isolated from the pulmonary and hepatic hydatid cysts of bubalus bubalis were partially purified on sephadex g-200 column. the isolated fractions were tested for their antigenicity by immunodot using the sera collected from experimentally infected puppies during the prepatent period of infection. six protein profiles (f1-f6) were recovered from both the isolates but the polypeptide recovery of each fraction of the two i ... | 2001 | 11247049 |
| behaviour of indian river buffaloes (bubalus bubalis) during short-haul road transportation. | 2001 | 11315139 | |
| a comparative evaluation of parasitological tests and a pcr for trypanosoma evansi diagnosis in experimentally infected water buffaloes. | in this study five parasitological methods and a polymerase chain reaction (pcr) were compared for the diagnostic sensitivity for trypanosoma evansi in experimentally infected water buffaloes over a period of 15 weeks. the combined estimates of sensitivity (ce(se)) of the pcr proved to be highest at 78.2%, closely followed by the mouse inoculation (mi), the micro-haematocrite centrifugation technique (mhct) and the mini-anion-exchange centrifugation technique (maect) with ce(se) of, respectively ... | 2001 | 11337124 |
| mutation and recombination in cattle satellite dna: a feedback model for the evolution of satellite dna repeats. | the cattle genome contains several distinct centromeric satellites with interrelated evolutionary histories. we compared these satellites in bovini species that diverged 0.2 to about 5 myr ago. quantification of hybridization signals by phosphor imaging revealed a large variation in the relative amounts of the major satellites. in the genome of water buffalo this has led to the complete deletion of satellite iii. comparative sequencing and pcr-rflp analysis of satellites iv, 1.711a, and 1.711b f ... | 2001 | 11343132 |
| pharmacokinetics and dosage regimen of 2-pyridine aldoxime in bubalus bubalis intoxicated with fenitrothion. | in the present study, the pharmacokinetics of 2-pyridine aldoxime (2-pam, 30 mg/kg, i.v.) alone and in conjunction with atropine (0.3 mg/kg; 1/4 i.v., 3/4 i.m.) was investigated in 10 bubalus bubalis intoxicated with a single oral lethal dose of fenitrothion (435 mg/kg). based on the kinetic parameters, an appropriate dosage regimen of 2-pam in b. bubalis was calculated. there was no significant difference between plasma levels and pharmacokinetic parameters of 2-pam in the two groups of animals ... | 2001 | 11348483 |
| 50,xy gonadal dysgenesis (swyer's syndrome) in a female river buffalo (bubalus bubalis). | 2001 | 11394803 | |
| fish-mapping of 31 type i loci (texas markers) to river buffalo chromosomes. | 2001 | 11419797 | |
| analysis of the 18s rrna genes of sarcocystis species suggests that the morphologically similar organisms from cattle and water buffalo should be considered the same species. | 2001 | 11420115 | |
| suitability of animals' purified milk caseins and their subunit kappa-caseins as substrates for subtilisin and trypsin. | acid casein and kappa-casein were purified from different species of animal's milk, such as cow, sheep, goat, and water buffalo. these caseins were used as substrates for commercially available subtilisin and trypsin. it was established that, when acid caseins were used as a substrate for subtilisin, cow acid casein was found to be a better substrate for the enzymes, compared to other animals' milk casein. it was suggested that this acid casein has significantly more aromatic amino acids, as com ... | 2001 | 11426702 |
| intramuscular kinetics and dosage regimens for pralidoxime in buffalo calves (bubalus bubalis). | the plasma levels, disposition kinetics and a dosage regimen for pralidoxime (2-pam) were investigated in male buffalo calves following single intramuscular administration (15 or 30 mg/kg). the effects of 2-pam on various blood enzymes were also determined. the absorption half-life, elimination half-life, apparent volume of distribution and total body clearance of 2-pam were 1.08 +/- 0.19 h, 3.14-3.19 h, 0.83-1.01 l/kg and 184.9-252.1 ml/(kg h), respectively. at doses of 15 and 30 mg/kg body wei ... | 2001 | 11432431 |
| current status of the river buffalo (bubalus bubalis l.) gene map. | ninety-nine loci have been assigned to river buffalo chromosomes, 67 of which are coding genes and 32 of which are anonymous dna segments (microsatellites). sixty-seven assignments were based on cosegregation of cellular markers in somatic cell hybrids (synteny), whereas 39 were based on in situ hybridization of fixed metaphase chromosomes with labeled dna probes. seven loci were assigned by both methods. of the 67 assignments in somatic cell hybrids, 38 were based on polymerase chain reaction ( ... | 2001 | 11447236 |
| twelve loci from hsa10, hsa11 and hsa20 were comparatively fish-mapped on river buffalo and sheep chromosomes. | ten type i loci from hsa10 (il2ra and vim), hsa11 (hbb and fshb) and hsa20 (thbd, avp/oxt, gnas1, hck and top1) and two domestic cattle type ii loci (cssm30 and bl42) were fish mapped to r-banded river buffalo (bbu) and sheep (oar) chromosomes. il2ra (hsa10) maps on bbu14q13 and oar13q13, vim (hsa10) maps on bbu14q15 and oar13q15, hbb (hsa11) maps on bbu16q25 and oar15q23, fshb (hsa11) maps on bbu16q28 and oar15q26, thbd (hsa20) maps on bbu14q15 and oar13q15 while avp/oxt, gnas1, hck, and top1 ( ... | 2001 | 11474194 |
| detection of anti-listeriolysin o and listeria monocytogenes in experimentally infected buffaloes (bubalus bubalis). | the kinetics of antibody production against listeriolysin o (allo) and the recovery pattern of listeria monocytogenes from bacteriological samples were studied following oral infection of buffalo calves with 3 x 10(9) cells each of pathogenic l. monocytogenes. antibodies to llo appeared by 7-10 days post infection (pi), with a shallow peak between days 16 and 36 pi, when tested by indirect plate-elisa. the titres of allo in all the animals then declined slowly but remained detectable up to day 7 ... | 2001 | 11474862 |
| indirect elisa for the diagnosis of brucellosis in water buffaloes (bubalus bubalis) in italy. | 2001 | 11497402 | |
| seroprevalence of neospora caninum in female water buffaloes (bubalus bubalis) from the southeastern region of brazil. | antibodies to neospora caninum were assayed in sera of 222 female water buffaloes from ribeira valley of são paulo state, brazil, using an indirect fluorescent antibody test (ifat) and neospora agglutination test (nat). ifat antibodies were found in 64% of buffaloes with titers of 1:25 (42 buffaloes), 1:50 (53 buffaloes), 1:100 (31 buffaloes), 1:200 (10 buffaloes), 1:400 (3 buffaloes), or > or =1:800 (3 buffaloes). nat antibodies were found in 53% of buffaloes; in titers of 1:40 in 52 buffaloes, ... | 2001 | 11511420 |
| identification of sarcocystis hominis-like (protozoa: sarcocystidae) cyst in water buffalo (bubalus bubalis) based on 18s rrna gene sequences. | dna templates were extracted from isolates of sarcocystis hominis-like cysts collected from cattle and water buffalo, as well as from sarcocystis fusiformis cysts and sarcocystis suihominis cysts. the 18s rrna genes were amplified using dna from a single cyst as the templates. approximately 1,367-1,440 bp sequences were obtained. the sequence difference in isolates of sarcocystis hominis-like cysts from water buffaloes, and isolates of s. hominis cysts from cattle were very low, only about 0.1%, ... | 2001 | 11534667 |
| the effect of gonadotropin releasing hormone on superovulation in elite swamp buffalo cows (bubalus bubalis). | the effect of gonadotropin releasing hormone (gnrh) supplement was investigated in twenty eight fsh-treated buffalo cows. animals were assigned to three groups; group i: gnrh was given at standing heat (n=9), group ii: gnrh was given 8-12 hr after standing heat (n=8) and group iii: control group with fsh alone (n=11). the responses (no. of corpora lutea and no. of anovulatory follicles), the number of recovered embryos and transferable embryos among the three groups were compared following slaug ... | 2001 | 11558539 |
| primary structure of alpha-globin chains from river buffalo (bubalus bubalis l.) hemoglobins. | primary structure analysis of the four river buffalo alpha-globin chains showed that haplotypes a and b differ from each other by a substitution at codon 64 that may encode ala or asn. the a haplotype encodes two alpha-globin chains, ialpha1 and iialpha3, which differ at positions 129 and 131: ialpha1 has 64 ala, 129 phe, 131 asn; iialpha3 has 64 ala, 129 leu, 131 ser. the b haplotype encodes two alpha-globin chains, ialpha2 and iialpha4, which differ at positions 10 and 11: ialpha2 has 10 i1e, ... | 2001 | 11563698 |
| purification and characterization of a bacteriocin-like compound (lichenin) produced anaerobically by bacillus licheniformis isolated from water buffalo. | to characterize a bacteriocin-like factor from bacillus licheniformis 26 l-10/3ra isolated from buffalo rumen. | 2001 | 11576300 |
| cytochrome b gene for species identification of the conservation animals. | a partial dna sequence of cytochrome b gene was used to identify the remains of endangered animals and species endemic to taiwan. the conservation of animals species included in this study were: the formosan gem-faced civets, leopard cats, tigers, clouded leopards, lion, formosan muntjacs, formosan sika deers, formosan sambars, formosan serows, water buffalo, formosan pangolins and formosan macaques. the control species used included domestic cats, domestic dogs, domestic sheeps, domestic cattle ... | 2001 | 11587860 |
| yeasts from water buffalo mozzarella, a traditional cheese of the mediterranean area. | countries of the mediterranean area are characterized by production of artisanal cheeses, obtained from goat, sheep, cow and buffalo raw milk. the numbers and species of yeasts in the different cheeses are variable, but some species are more frequently detected than others. kluyveromyces marxianus, k. lactis with their anamorph, candida kefir, debaryomyces hansenii and c. famata, c. colliculosa and c. catenulata are dominant species in several cheeses. however, saccharomyces cerevisiae is often ... | 2001 | 11589559 |
| identification of adulteration in milk by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | the development is described of a rapid, simply and accurate analytical method aimed at evaluating both the presence of cow milk in either raw ewe and water buffalo milk samples employed in industrial processes and the addition of powdered milk to samples of fresh raw milk, using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms). the presence of adulteration is defined by evaluating the protein patterns coming from the most abundant whey proteins, alpha-l ... | 2001 | 11599081 |
| laboratory and field evaluation of schistosoma japonicum dna vaccines in sheep and water buffalo in china. | vaccines are needed to control zoonotic schistosoma japonicum infection and several vaccine candidates have now been identified. two of these (sj28gst and sj23) have shown particular promise in sheep when injected with freund's adjuvants. the objective of the present work was to find a vaccine formulation which may have potential for widespread use in the field. dna vaccine formulations of these antigens were produced and tested first in sheep under laboratory conditions and then in both the lab ... | 2001 | 11672910 |