Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| effect of intensive handwashing in the prevention of diarrhoeal illness among patients with aids: a randomized controlled study. | patients with aids frequently develop diarrhoeal illness. in this randomized, controlled study, 260 patients were screened for those who had not had diarrhoea in the preceding 3 months and who had received a stable highly active antiretroviral therapy regimen for at least 6 weeks prior to the study enrollment. a total of 148 patients met the inclusion criteria and were enrolled: 75 patients were randomly assigned to an intensive handwashing intervention (i.e. handwashing after defecation, after ... | 2007 | 17446290 |
| cryptosporidiosis among immunocompetent patients with gastroenteritis in iran: a comparison with other enteropathogenic parasites. | cryptosporidium is a protozoan parasite that reproduces within the epithelial cells of several organs of vertebrate hosts. the manifestation of the disease is either self-limiting acute diarrhea in immunocompetent patients, or fatal chronic diarrhea in immunocompromised patients. common clinical symptoms include watery diarrhea, abdominal pain, and weight loss. | 2007 | 17446964 |
| thioredoxin-linked metabolism in entamoeba histolytica. | entamoeba histolytica, an intestinal protozoan that is the causative agent of amoebiasis, is exposed to elevated amounts of highly toxic reactive oxygen species during tissue invasion. in this work, we report the molecular cloning, from e. histolytica genomic dna, of the genes ehtrxr and ehtrx41, respectively coding for thioredoxin reductase (ehtrxr) and thioredoxin (ehtrx41). the genes were expressed in escherichia coli cells, and the corresponding recombinant proteins were purified and charact ... | 2007 | 17448896 |
| a novel amoeba proteus 120 kda actin-binding protein with only 1 filamin repeat and a coiled-coil region. | a novel 120 kda actin-binding protein (apabp-f1) was found in amoeba proteus. it was distributed throughout the cytoplasm, mainly in the subplasma membrane and perinuclear-nuclear areas, enriched in actin. the full-length cdna of apabp consisted of 2672 nucleotides with an open reading frame of 878 amino acids, giving a ~95 kda protein with a theoretical pi value of 5.11. it had a novel domain organization pattern: the n terminus (residues 1-104) contained 1 calponin-homology (ch) domain, follow ... | 2007 | 17464342 |
| activation of dendritic cells by the gal-lectin of entamoeba histolytica drives th1 responses in vitro and in vivo. | amebiasis is a human disease caused by the protozoan intestinal parasite entamoeba histolytica. vaccine development has focused on the parasite's surface galactose-n-acetyl-d-galactosamine inhibitable lectin (gal-lectin) as a protective antigen. the gal-lectin is immunogenic and has been shown to induce th1 cytokines in vitro and in vivo. the immunological basis of the protective immune response elicited by the gal-lectin is unknown. in this study, we investigated the response of balb/c bone mar ... | 2007 | 17219364 |
| current therapeutics, their problems, and sulfur-containing-amino-acid metabolism as a novel target against infections by "amitochondriate" protozoan parasites. | the "amitochondriate" protozoan parasites of humans entamoeba histolytica, giardia intestinalis, and trichomonas vaginalis share many biochemical features, e.g., energy and amino acid metabolism, a spectrum of drugs for their treatment, and the occurrence of drug resistance. these parasites possess metabolic pathways that are divergent from those of their mammalian hosts and are often considered to be good targets for drug development. sulfur-containing-amino-acid metabolism represents one such ... | 2007 | 17223627 |
| pcr detection of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii in stool samples from sydney, australia. | this study investigated the presence of entamoeba histolytica, entamoeba dispar, and entamoeba moshkovskii in stool samples from a patient population in sydney, australia. stool samples were tested by microscopy and pcr. five patients were found with e. histolytica infections, while e. dispar and e. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by pcr. this is the first study in australia using molecular techniques to determine the presence of e. histolytica, e. ... | 2007 | 17229864 |
| fulminant amoebic colitis following loperamide use. | toxic megacolon is a rare consequence of infection with entamoeba histolytica. we present such a patient in whom the course of disease may have been influenced by heavy loperamide use. loperamide and other anti-motility agents have been implicated previously in the pathogenesis of toxic megacolon in patients with infectious gastroenteritis. | 2007 | 17241255 |
| identification of developmentally regulated genes in entamoeba histolytica: insights into mechanisms of stage conversion in a protozoan parasite. | developmental switching between life-cycle stages is a common feature among many pathogenic organisms. the protozoan parasite entamoeba histolytica converts between cysts (essential for disease transmission) and trophozoites (responsible for tissue invasion). identification of genes involved in the developmental pathway has been severely hindered by the inability to generate e. histolytica cysts in vitro. using parasite strains derived from recent human infections and whole-genome transcriptiona ... | 2007 | 17250591 |
| luminal host-defense mechanisms against invasive amebiasis. | most humans infected with the virulent protozoan parasite entamoeba histolytica do not develop invasive disease. available evidence indicates that beneficial bacteria and the mucus gel layer in the colon lumen protect the host mucosa. glycosidases produced by some normal colonic bacteria and luminal proteases degrade the key adherence lectin on e. histolytica trophozoites and decrease their adherence to epithelial cells. the mucus gel layer prevents those trophozoites that escape the hydrolases ... | 2007 | 17258936 |
| growth of the protozoan parasite entamoeba histolytica in 5-azacytidine has limited effects on parasite gene expression. | in higher eukaryotes dna methylation regulates important biological functions including silencing of gene expression and protection from adverse effects of retrotransposons. in the protozoan parasite entamoeba histolytica, a dna methyltransferase has been identified and treatment with 5-azacytidine (5-azac), a potent inhibitor of dna methyltransferase, has been reported to attenuate parasite virulence. however, the overall extent of dna methylation and its subsequent effects on global gene expre ... | 2007 | 17207281 |
| induction of monocyte chemotactic protein 1 in colonic epithelial cells by entamoeba histolytica is mediated via the phosphatidylinositol 3-kinase/p65 pathway. | the role intestinal epithelial cells play in the pathogenesis of amebic colitis is poorly understood. herein, we demonstrate that secreted and soluble ameba (entamoeba histolytica) proteins (sap) induce expression of the chemoattractant monocyte chemotactic protein (mcp) in the colonic epithelial cell lines caco-2, t84, and ls174t. mcp-1 mrna induction was both dose and time dependent, with peak induction occurring at 8 h and with 100 mug/ml of sap. significant increase in mcp-1 protein expressi ... | 2007 | 17283105 |
| correlation of interferon-gamma production by peripheral blood mononuclear cells with childhood malnutrition and susceptibility to amebiasis. | the contribution of interferon-gamma (ifn-gamma) to immunity from amebiasis was assessed in a three-year prospective study of children 2-5 years of age in an urban slum of dhaka, bangladesh. ifn-gamma produced by peripheral blood mononuclear cells stimulated with soluble amebic antigen was measured upon enrollment. thirty-one of the 209 enrolled children had entamoeba histolytica-associated diarrhea. children who produced higher than the median level of ifn-gamma (median = 580 pg/ml) had longer ... | 2007 | 17297046 |
| onchocerca volvulus-specific antibody and cytokine responses in onchocerciasis patients after 16 years of repeated ivermectin therapy. | the recommended control option against onchocerciasis is repeated ivermectin treatment, which will need to be implemented for decades, and it remains unknown how repeated ivermectin therapy might affect immunity against onchocerca volvulus in the long term. o. volvulus-specific antibody reactivity and cellular cytokine production were investigated in onchocerciasis patients receiving ivermectin (150 microg/kg) annually for 16 years. in treated patients, the t helper type 2 (th2) cytokine interle ... | 2007 | 17302900 |
| the 29-kilodalton thiol-dependent peroxidase of entamoeba histolytica is a factor involved in pathogenesis and survival of the parasite during oxidative stress. | the 29-kda surface antigen (thiol-dependent peroxidase; eh29) of entamoeba histolytica exhibits peroxidative and protective antioxidant activities. during tissue invasion, the trophozoites are exposed to oxidative stress and need to deal with highly toxic reactive oxygen species (ros). in this investigation, attempts have been made to understand the role of the 29-kda peroxidase gene in parasite survival and pathogenesis. inhibition of eh29 gene expression by antisense rna technology has shown a ... | 2007 | 17307964 |
| magnesium promotes structural integrity and conformational switching action of a calcium sensor protein. | calcium binding proteins carry out various signal transduction processes upon binding to ca2+. in general, these proteins perform their functions in a high background of mg2+. here, we report the role of mg2+ on a calcium sensor protein from entamoeba histolytica (ehcabp), containing four ca2+-binding sites. mg2+-bound ehcabp exists as a monomer with a conformation different from that of the holo- and apo-ehcabp. nmr and biophysical data on ehcabp demonstrate that mg2+ stabilizes the closed conf ... | 2007 | 17315953 |
| importance of the detection of amoebic antigens in stool samples for the diagnosis of entamoeba histolytica infection, among children in southern turkey. | entamoeba histolytica is the predominant causative agent of human amoebiasis, a significant and common diarrhoeal disease among children of developing countries. diagnosis of this illness by the microscopical detection of the parasites in stool samples is insensitive and often incapable of differentiating the pathogenic entamoeba histolytica from the commensal parasite e. dispar. in this study, the results of testing stool samples in an elisa, based on a monoclonal antibody, that detects e. hist ... | 2007 | 17316500 |
| differentiation of entamoeba histolytica and entamoeba dispar by pcr: a preliminary study in izmir, turkey. | the causative agent of amoebiasis is currently attributed to two distinct species (e. histolytica and e. dispar). the aim of this study was to differentiate these species by pcr in stool samples. isolated genomic dna was amplified by pcr and band products of 101 bp (e. dispar) were obtained. all seven stool samples were found to be e. dispar, not e. histolytica. our results demonstrated the significance of e. histolytica/dispar differentiation in the diagnosis of amoebiasis. this study is prelim ... | 2007 | 17319599 |
| ehgef3, a novel dbl family member, regulates ehraca activation during chemotaxis and capping in entamoeba histolytica. | rho gtpases are critical elements involved in the regulation of signal transduction cascades from extracellular stimuli to cytoskeleton. the rho guanine nucleotide exchange factors (rhogefs) have been implicated in direct activation of these gtpases. here, we describe a novel rhogef, denominated ehgef3 from the parasite entamoeba histolytica, which encodes a 110 kda protein containing the domain arrangement of a dbl homology domain in tandem with a pleckstrin homology domain, the dh domain of eh ... | 2007 | 17323375 |
| enteroparasitic occurrence in stools from residents in southwestern region of saudi arabia before and during umrah season. | study of the prevalence of human gastro-intestinal parasitic infections among patients living in makkah al-mukkarmah city before and during umrah season. | 2007 | 17334465 |
| entamoeba histolytica: apoptosis induced in vitro by nitric oxide species. | apoptosis has been described in some parasites like leishmania, trypanosoma, and trichomonas. this phenomenon has not been observed yet in entamoeba histolytica. this work analyzed the in vitro effect of sodium nitroprusside, sodium nitrite and sodium nitrate (nos) on e. histolytica apoptosis. parasites incubated for 1h with nos revealed apoptosis 6h later (95% viability), demonstrated by yopro-1, tunel, dna fragmentation and low atp levels. the caspase inhibitor z-vad-fmk inhibited total intrac ... | 2007 | 17336295 |
| nitric oxide production and nitric oxide synthase immunoreactivity in naegleria fowleri. | free-living ameba naegleria fowleri produces an acute and fatal infectious disease called primary amebic meningoencephalitis (pam), whose pathophysiological mechanism is largely unknown. the aim of this study was to investigate the role of nitric oxide (no) in pam. although no has a cytotoxic effect on various parasites, it is produced by others as part of the pathology, as is the case with entamoeba histolytica. to test for the production of no, we analyzed whether antibodies against mammalian ... | 2007 | 17340143 |
| stable expression of gal/galnac lectin of entamoeba histolytica in transgenic chloroplasts and immunogenicity in mice towards vaccine development for amoebiasis. | chloroplast genetic engineering offers several advantages, including high levels of transgene expression, transgene containment via maternal inheritance and multigene engineering in a single transformation event. entamoeba histolytica infects 50 million people, causing about 100,000 deaths annually, but there is no approved vaccine against this pathogen. leca, a potential target for blocking amoebiasis, was expressed for the first time in transgenic plants. stable transgene integration into chlo ... | 2007 | 17309678 |
| identification of putative transcriptional regulatory networks in entamoeba histolytica using bayesian inference. | few transcriptional regulatory networks have been described in non-model organisms. in entamoeba histolytica seminal aspects of pathogenesis are transcriptionally controlled, however, little is known about transcriptional regulatory networks that effect gene expression in this parasite. we used expression data from two microarray experiments, cis-regulatory motif elucidation, and a naïve bayesian classifier to identify genome-wide transcriptional regulatory patterns in e. histolytica. our algori ... | 2007 | 17355990 |
| comparison of entamoeba histolytica dna isolated from a cynomolgus monkey with human isolates. | three protein-coding loci in dna of an entamoeba histolytica strain (ehmfas1) isolated from cynomolgus monkey (macaca fascicularis) were sequenced; these loci corresponded to the genes for chitinase, the serine-rich e. histolytica protein (srehp), and the 16 s-like small subunit ribosomal rna (16s-like ssurrna). the nucleotide and deduced amino-acid sequences of chitinase and srehp were compared with sequences from human isolates. ehmfas1 had several specific mutations in units in the polymorphi ... | 2007 | 17356893 |
| two rab7 isotypes, ehrab7a and ehrab7b, play distinct roles in biogenesis of lysosomes and phagosomes in the enteric protozoan parasite entamoeba histolytica. | rab7 small gtpase plays a crucial role in the regulation of trafficking to late endosomes, lysosomes and phagosomes. while most eukaryotes encode a single rab7, the parasitic protist entamoeba histolytica possesses nine rab7. in this study, to understand the significance of the presence of multiple rab7 isotypes, a role of two representative rab7 isotypes, ehrab7a and ehrab7b, was investigated. ehrab7b was exclusively localized to acidic vacuoles containing lysosomal proteins, e.g. amoebapore-a ... | 2007 | 17359234 |
| prevalence of enteric protozoa in human immunodeficiency virus (hiv)-positive and hiv-negative men who have sex with men from sydney, australia. | a prospective, comparative study of the prevalence of enteric protozoa was determined among human immunodeficiency virus (hiv)- positive and hiv-negative men who have sex with men (msm) in sydney, australia. a total of 1,868 patients submitted stool specimens; 1,246 were from msm (628 hiv positive and 618 hiv positive) and 622 from non-msm were examined over a 36-month period. a total of 651 (52.2%) stool specimens from msm were positive for protozoa compared with 85 (13%) from non-msm. there wa ... | 2007 | 17360882 |
| late experimental amebic liver abscess in hamster is inhibited by cyclosporine and n-acetylcysteine. | during early experimental amebic liver abscess in hamsters (ealah), acute inflammation is primarily responsible for tissue damage. however, during the late stages of this process, the relative contribution to tissue destruction of both parasite factors and host response is unknown. in the present work, the role of the cellular immune response in tissue damage during ealah is explored by using the immunosuppressor drug cyclosporine a (csa). csa treatment inhibits tissue damage after 72 h (but not ... | 2007 | 17362925 |
| transcriptional and secretory responses of entamoeba histolytica to mucins, epithelial cells and bacteria. | invasive intestinal amebiasis, caused by entamoeba histolytica, is initiated with attachment of trophozoites to the colonic mucous layer, mucous disruption and/or depletion, and adherence to and cytolysis of host epithelial and inflammatory cells. a current working model of intestinal amebiasis suggests that the microenvironment of the host intestine, particularly intestinal mucins and the bacterial biofilm, may influence the behavior of pathogenic amebae. the invasive phenotype is dependent on ... | 2007 | 17362964 |
| entamoeba histolytica encephalitis diagnosed by pcr of cerebrospinal fluid. | extra-intestinal amebiasis is secondary to invasive intestinal infections and usually results in an amebic liver abscess. other organs, including lungs, brain, skin, spleen and kidneys, may be involved. diagnosis of the cerebral infection is of the utmost importance and is most often made by detection of the organism at the time of brain biopsy or at autopsy. we report the first case of entamoeba histolytica encephalitis diagnosed by pcr of the cerebrospinal fluid. the patient was treated succes ... | 2007 | 16930651 |
| eh klp5 is a divergent member of the kinesin 5 family that regulates genome content and microtubular assembly in entamoeba histolytica. | earlier studies have established two unusual features in the cell division cycle of entamoeba histolytica. first, microtubules form a radial assembly instead of a bipolar mitotic spindle, and second, the genome content of e. histolytica cells varied from 1x to 6x or more. in this study, eh klp5 was identified as a divergent member of the bimc kinesin family that is known to regulate formation and stabilization of the mitotic spindle in other eukaryotes. in contrast to earlier studies, we show he ... | 2007 | 16925786 |
| a phagocytosis mutant of entamoeba histolytica is less virulent due to deficient proteinase expression and release. | cysteine proteinases are key virulence factors of entamoeba histolytica that are released during the process of invasion. we used a chemical mutant of e. histolytica strain hm-1:imss, clone l6, which is deficient in virulence, phagocytosis, and cysteine proteinase activity to help define the mechanisms of cysteine proteinase release. all cysteine proteinase genes of wild type hm-1 were present in the l6 mutant genome, but three of the major expressed proteinases, ehcp1, ehcp2, and ehcp5 were bot ... | 2007 | 16987516 |
| aberrant induction of regulatory activity of cd4+cd25+ t cells by dendritic cells in hiv-infected persons with amebic liver abscess. | to know why hiv-1-infected persons are particularly susceptible to amebic liver abscess (ala), we investigated the role of cd4cd25 t cells in the susceptibility of hiv-1-infected persons to this disease. herein we show, in early stage hiv-1-infected subjects, that cd4 t-cell responses to entamoeba histolytica antigen (ehag) were selectively impaired, especially in those with ala. ehag-specific cd4 t-cell responses were normalized by depletion of cd4cd25 cells or by addition of anti-cytotoxic t l ... | 2007 | 17003690 |
| proteomic analysis of entamoeba histolytica. | in this study, the proteome of axenically grown entamoeba histolytica parasites was explored by two-dimensional gel electrophoresis (2-de), employing a practical and effective procedure for the solubilization of e. histolytica proteins. approximately 900 protein species in the ph range between 4 and 7 were detected by coomassie blue staining. ninety-five spots were excised, trypsinated and subjected to mass spectrometry. the resultant data from peptide mass fingerprints were compared with those ... | 2007 | 17032470 |
| a single proline substitution is critical for the thermostabilization of clostridium beijerinckii alcohol dehydrogenase. | analysis of the three-dimensional structures of three closely related mesophilic, thermophilic, and hyperthermophilic alcohol dehydrogenases (adhs) from the respective microorganisms clostridium beijerinckii (cbadh), entamoeba histolytica (ehadh1), and thermoanaerobacter brockii (tbadh) suggested that a unique, strategically located proline residue (pro100) might be crucial for maintaining the thermal stability of ehadh1. to determine whether proline substitution at this position in tbadh and cb ... | 2007 | 17063493 |
| irritable bowel syndrome: a review on the role of intestinal protozoa and the importance of their detection and diagnosis. | irritable bowel syndrome (ibs) is a functional gastrointestinal disorder in which abdominal pain is associated with a defect or a change in bowel habits. gut inflammation is one of the proposed mechanisms of pathogenesis. recent studies have described a possible role for protozoan parasites, such as blastocystis hominis and dientamoeba fragilis, in the etiology of ibs. dientamoeba fragilis is known to cause ibs-like symptoms and has a propensity to cause chronic infections but its diagnosis reli ... | 2007 | 17070814 |
| molecular modeling of some 1h-benzimidazole derivatives with biological activity against entamoeba histolytica: a comparative molecular field analysis study. | comparative molecular field analysis (comfa) was performed on a set of 1h-benzimidazole derivatives. molecular modeling and 3d-qsar were employed to determine the tautomeric form that would probably fit a target receptor in entamoeba histolytica. comfa results suggest that the antiamoebic activity is favored with steric bulk at position 5 of the benzimidazole ring and low electron density on the group at position 2. to the best of our knowledge this is the first 3d-qsar study performed for benzi ... | 2007 | 17074492 |
| subcellular distribution of the entamoeba histolytica 140 kda fn-binding molecule during host-parasite interaction. | entamoeba histolytica trophozoites recovered from the host-parasite interface during abscess development obtain different stimuli compared with long-term cultured cells. in order to have a better understanding about the mechanisms in which the 140 kda fibronectin (fn)-binding molecule (ehfnr) is involved during the invasive process, we decided to compare the regulation process of this molecule among long-term cultured trophozoites, fn-stimulated trophozoites, and trophozoites recently recovered ... | 2007 | 17076927 |
| additional antiprotozoal flavonol glycosides of the aerial parts of helianthemum glomeratum. | bioassay-guided fractionation of the methanol extract of aerial parts from helianthemum glomeratum afforded five antiprotozoal flavonol glycosides: tiliroside, kaempferol-3-o-(3'',6''di-o-e-p-coumaroyl)-betad-glucopyranoside, astragalin, quercitrin and isoquercitrin. the in vitro antiprotozoal assay showed that tiliroside was the most potent antiamoebic and antigiardial compound with ic(50) values of 17.5 microg/ml for entamoeba histolytica and 17.4 microg/ml for g. lamblia. isoquercitrin showed ... | 2007 | 17094176 |
| entamoeba histolytica: an ecto-phosphatase activity regulated by oxidation-reduction reactions. | in this work, an ecto-phosphatase activity of entamoeba histolytica was characterized using intact cells. this activity presented the following biochemical characteristics: (i) it hydrolyzes p-npp with v(max) of 8.00+/-0.22 nmol p-np x h(-1) x 10(-5) cells and k(m) of 2.68+/-0.25 mm; (ii) it is inhibited by acid phosphatase inhibitors, such as sodium molybdate (k(i)=1.70+/-0.24 microm) and sodium fluoride (k(i)=0.25+/-0.02 mm); (iii) it also showed high sensitivity to phosphotyrosine phosphatase ... | 2007 | 17113080 |
| evidence for a link between parasite genotype and outcome of infection with entamoeba histolytica. | the factors determining whether a person infected with entamoeba histolytica develops disease remain obscure. to investigate whether the parasite genome contributes to the outcome, we have investigated the distribution of parasite genotypes among e. histolytica-infected individuals in bangladesh. samples were obtained from individuals who either were asymptomatic, had diarrhea/dysentery, or had developed a liver abscess. genotypes were determined by using six trna-linked polymorphic markers, and ... | 2007 | 17122021 |
| ehgef2, a dbl-rhogef from entamoeba histolytica has atypical biochemical properties and participates in essential cellular processes. | dbl proteins are a family of factors that exchange the guanine nucleotide which promote the activation of rho small gtpases. this paper reports the molecular, structural, biochemical and functional characterization of ehgef2, a new member of the dbl family. ehgef2 is the second gef studied in parasites and in the protozoan entamoeba histolytica, and it is also the first member of the dbl family that was found to have arm repeats. the catalytic domain (dh) of ehgef2 has the conserved residues t42 ... | 2007 | 17125853 |
| transcriptomic comparison of two entamoeba histolytica strains with defined virulence phenotypes identifies new virulence factor candidates and key differences in the expression patterns of cysteine proteases, lectin light chains, and calmodulin. | the availability of rahman, and the virulent hm-1:imss strain of e. histolytica, provides a powerful tool for identifying virulence factors of e. histolytica. here we report an attempt to identify potential virulence factors of e. histolytica by comparing the transcriptome of e. histolytica hm-1:imss and e. histolytica rahman. with phenotypically defined strains, we compared the transcriptome of rahman and hm-1:imss using a custom 70mer oligonucleotide based microarray that has essentially full ... | 2007 | 17141337 |
| syntheses, characterization and in vitro antiamoebic activity of new pd(ii) complexes with 1-n-substituted thiocarbamoyl-3,5-diphenyl-2-pyrazoline derivatives. | reaction of neutral ns bidentate ligands, 1-n-substituted thiocarbamoyl-3,5-diphenyl-2-pyrazolines, isolated by cyclization of chalcone with n-4-substituted thiosemicarbazide of aromatic amines (1-8), with [pd(dmso)(2)cl(2)] (dmso=dimethylsulfoxide) leads to the formation of new complexes of the type [pd(l)cl(2)] (1a-8a). the structures of the compounds were elucidated by uv, ir, (1)h nmr, (13)c nmr and esi-ms spectral data and thermogravimetric analysis and their purities were confirmed by elem ... | 2007 | 17156895 |
| antiparasitic drug nitazoxanide inhibits the pyruvate oxidoreductases of helicobacter pylori, selected anaerobic bacteria and parasites, and campylobacter jejuni. | nitazoxanide (ntz) exhibits broad-spectrum activity against anaerobic bacteria and parasites and the ulcer-causing pathogen helicobacter pylori. here we show that ntz is a noncompetitive inhibitor (k(i), 2 to 10 microm) of the pyruvate:ferredoxin/flavodoxin oxidoreductases (pfors) of trichomonas vaginalis, entamoeba histolytica, giardia intestinalis, clostridium difficile, clostridium perfringens, h. pylori, and campylobacter jejuni and is weakly active against the pyruvate dehydrogenase of esch ... | 2007 | 17158936 |
| isolation of pure compound r/j/3 from pluchea indica (l.) less. and its anti-amoebic activities against entamoeba histolytica. | the plant pluchea indica is known for its anti-inflammatory, anti-ulcer, anti-pyretic, hypoglycemic, diuretic and anti-microbial activities besides many other pharmacological activities. we have isolated and purified seven compounds from the methanolic root extract of this plant by column chromatography. the compounds were identified by spectroscopic analyses. the anti-amoebic activities of the pure compound r/j/3 was investigated against the hm1 strain of entamoeba histolytica. the compound, r/ ... | 2007 | 17174538 |
| sine elements of entamoeba dispar. | entamoeba histolytica and e. dispar are closely related protozoan parasites; the former causes clinical amoebiasis in humans while the latter appears to be non-pathogenic. the molecular biology of e. histolytica shows a number of unusual features, one of which is the abundance of polyadenylated but apparently untranslatable mrnas produced; many of these are the product of at least three families of sines (ehsine1-3). here we show that the genome of e. dispar contains numerous copies of a sine el ... | 2007 | 17178164 |
| incidence of cyclosporiasis in patients with gastrointestinal symptoms in western turkey. | this study was designed to investigate the distribution of cyclosporiasis between october 2003 and october 2004 and the relationship between cyclospora infection and seasonal as well as patient factors in western turkey. | 2007 | 17179908 |
| innate resistance to entamoeba histolytica in murine models. | hamano et al. have recently reported that in murine models of amebiasis, innate resistance is associated with nonhemopoietic cells and depends on the activity of interleukin-10. this article raises the issue of the importance of predisposition to amebic colitis and discusses which host factors have a role in innate resistance to invasive disease. resistance studies that elucidate the mechanisms involved in parasite clearance or parasite invasion are essential to understanding the incidence of am ... | 2007 | 17185037 |
| initiation of inflammation and cell death during liver abscess formation by entamoeba histolytica depends on activity of the galactose/n-acetyl-d-galactosamine lectin. | the parasite entamoeba histolytica colonizes the human intestine causing amoebic colitis and disseminates through the vascular route to form liver abscesses. the gal/galnac lectin is an adhesion protein complex which sustains tissue invasion by e. histolytica. disruption of the gal/galnac lectin function in engineered parasites (hgl-2 trophozoites) changed the pathophysiology of hamster liver abscess formation. hgl-2 trophozoites produced numerous small inflammatory foci located in the vicinity ... | 2007 | 17188278 |
| molecular methods for diagnosis of entamoeba histolytica in a clinical setting: an overview. | the range of clinical outcomes following entamoeba histolytica infection is likely to be influenced by the different strains of the parasite already existing in our population. there is a need for developing faster, reliable and reproducible methods for identifying the different strains of e. histolytica. this would have a major impact on the subsequent course of treatment given to patients. in the post-genomic era, different loci of the entamoeba genome have been targeted for developing suitabl ... | 2007 | 17189632 |
| colonic amoebiasis and spirochetosis: morphological, ultrastructural and microbiological evaluation. | the present study reports on a prompt diagnosis of colonic amoebiasis with colonic spirochetosis by brachyspira aalborgi and b. pilosicoli; such diagnosis allowed exclusion of other diseases and resolution of the case after specific treatment. | 2007 | 17201883 |
| profiles of a pathogenic entamoeba histolytica-like variant with variations in the nucleotide sequence of the small subunit ribosomal rna isolated from a primate (de brazza's guenon). | a pathogenic entamoeba histolytica-like variant (jsk2004) with genetic variations and a novel isoenzyme pattern was isolated from a de brazza's guenon. a homology of 99.1% was found between the clones of e. histolytica (hm-1:imss) and jsk2004 in the 1,893 nucleotide bases of the small subunit rrna (ssu-rrna) gene. the dna of the pathogenic amoeba species was also extracted from two sterile liver abscesses during the autopsies of an abyssinian colobus and a geoffroy's spider monkey occurring in t ... | 2007 | 17939358 |
| anti-amebic antibody activity in patients, determined with antigens prepared from virulent parasites (indirect hemagglutination assay and enzyme-linked immunosorbent assay). | the serology of amebiasis is affected by low sensitivity and specificity. | 2007 | 17939629 |
| [infectious aetiologies of travelers' diarrhoea]. | traveler's diarrhoea (td) occurs in 20 to 60% of european or north-american travelers in intertropical areas. following return from endemic zone, malaria must always be evocated in front of febrile diarrhoea. many causative infectious agents are involved in td and their frequency may vary according to destination and seasons. the main agents involved in td are escherichia coli pathovars (especially enterotoxigenic and enteroaggregative e. coli) followed by enteroinvasive bacteria (campylobacter ... | 2007 | 17942257 |
| invasive amebiasis and ameboma formation presenting as a rectal mass: an uncommon case of malignant masquerade at a western medical center. | a 54-year-old man presented with rectal pain and bleeding secondary to ulcerated, necrotic rectal and cecal masses that resembled colorectal carcinoma upon colonoscopy. these masses were later determined to be benign amebomas caused by invasive entamoeba histolytica, which regressed completely with medical therapy. in western countries, the occurrence of invasive protozoan infection with formation of amebomas is very rare and can mistakenly masquerade as a neoplasm. not surprisingly, there have ... | 2007 | 17948943 |
| invasive amebiasis: an update on diagnosis and management. | in its invasive form, the trophozoite is responsible for clinical syndromes, ranging from classical dysentery to extraintestinal disease with emphasis on hepatic amebiasis. abdominal pain, tenderness and diarrhea of watery stool, sometimes with blood, are the predominant symptoms of amebic colitis. besides the microscopic identification of entamoeba histolytica, diagnosis should be based on the detection of specific antigens in the stool or pcr associated with the occult blood in the stool. ameb ... | 2007 | 17914922 |
| [the evaluation of the techniques used for diagnosis of entamoeba histolytica in stool specimens]. | in this study, stool samples of 9378 patients from different clinics, who presented at the laboratory of the department of parasitology of the dokuz eylul university, faculty of medicine with several gastrointestinal complaints from january 2004 to may 2006, were examined. all stool samples were examined with the saline-lugol method and, in suspicious cases, by trichrome staining, cultivation in robinson's medium and/or antigen detection in stool with the entamoeba celisa path kit. forty-one cas ... | 2007 | 17918056 |
| [a retrospective analysis of the results of a seven-year parasitological examination of stools from malatya state hospital]. | the aim of this study was to determine the prevalence of intestinal parasites and to evaluate changes in the annual rates by retrospective screening of the data from our hospital's parasitology laboratory between the period of 2000 and 2006. parasitological examinations were done with native-lugol, cellophane-tape methods and trichrome staining in ambiguous cases, in a total of 67539 samples. the prevalence of the intestinal parasitosis during the years 2000 and 2006 years were found to be 7.2% ... | 2007 | 17918059 |
| drug targets from human pathogenic amoebas: entamoeba histolytica, acanthamoeba polyphaga and naegleria fowleri. | in this review we present our search for the presence of drug targets in several species of human pathogenic parasites, mainly the amoebas entamoeba histolytica, acanthamoeba polyphaga and naegleria fowleri. we started with an analysis of the concepts of essentiality and validity of the targets and continue with a description of the main characteristics of pathogenicity of these amoebas. we then proceed to evaluate these targets arranged mainly in seven groups corresponding to: a) enzymes which ... | 2007 | 17897063 |
| involvement of beta 2-integrin in ros-mediated neutrophil apoptosis induced by entamoeba histolytica. | cellular adhesion through beta 2-integrin (cd18) is an important step in signal transduction leading to apoptosis of human neutrophils, and nadph oxidase-derived reactive oxygen species (ros) are essential for neutrophil apoptosis induced by entamoeba histolytica. therefore, we investigated the role of beta 2-integrin-mediated signals in ros-dependent neutrophil apoptosis induced by e. histolytica. entamoeba-induced apoptosis was inhibited by pre-incubation of cells with mab to cd18, but not cd2 ... | 2007 | 17900957 |
| structural and functional analysis of the entamoeba histolytica ehrabb gene promoter. | the entamoeba histolytica ehrabb gene encodes for a rab gtpase involved in phagocytosis. it is located at a virulence locus where the ehcp112 gene is in the complementary strand at 332 bp of ehrabb start codon, suggesting a finely regulated transcription of both genes. however, the transcription regulation in this parasite is poorly understood. | 2007 | 17883848 |
| caenorhabditis elegans pgp-5 is involved in resistance to bacterial infection and heavy metal and its regulation requires tir-1 and a p38 map kinase cascade. | animals and plants respond to bacterial infections and environmental stresses by inducing overlapping repertoires of defense genes. how the signals associated with infection and abiotic stresses are differentially integrated within a whole organism remains to be fully addressed. we show that the transcription of a caenorhabditis elegans abc transporter, pgp-5 is induced by both bacterial infection and heavy metal stress, but the magnitude and tissue distribution of its expression differs, depend ... | 2007 | 17888400 |
| amoebiasis: current status in australia. | 2007 | 17874993 | |
| parasitic infections among orang asli (aborigine) in the cameron highlands, malaysia. | in april 2004, an outbreak of acute diarrheal illness occurred among the orang asli (aborigine) in the cameron highlands, pahang state, peninsular malaysia, where rotavirus was later implicated as the cause. in the course of the epidemic investigation, stool samples were collected and examined for infectious agents including parasites. soil transmitted helminthes (sth), namely ascaris lumbricoides (25.7%), trichuris trichiura (31.1%) and hookworm (8.1%), and intestinal protozoa, which included g ... | 2007 | 17877212 |
| primary structure, expression and localization of two intermediate subunit lectins of entamoeba dispar that contain multiple cxxc motifs. | we have recently identified 2 surface proteins in entamoeba histolytica as intermediate subunits of galactose- and n-acetyl-d-galactosamine-inhibitable lectin (ehigl1 and ehigl2); these proteins both contain multiple cxxc motifs. here, we report the molecular characterization of the corresponding proteins in entamoeba dispar, which is neither pathogenic nor invasive. two igl genes encoding 1110 and 1106 amino acids (edigl1 and edigl2) were cloned from 2 strains of e. dispar. the amino acid seque ... | 2007 | 17803840 |
| kinetic modeling can describe in vivo glycolysis in entamoeba histolytica. | glycolysis in the human parasite entamoeba histolytica is characterized by the absence of cooperative modulation and the prevalence of pyrophosphate-dependent (over atp-dependent) enzymes. to determine the flux-control distribution of glycolysis and understand its underlying control mechanisms, a kinetic model of the pathway was constructed by using the software gepasi. the model was based on the kinetic parameters determined in the purified recombinant enzymes, and the enzyme activities, and st ... | 2007 | 17824961 |
| identification of an entamoeba histolytica serine-, threonine-, and isoleucine-rich protein with roles in adhesion and cytotoxicity. | entamoeba histolytica is a leading cause of parasitic death globally. however, the molecular framework regulating pathogenesis is poorly understood. we have previously used expression profiling to identify entamoeba genes whose expressions were strictly associated with virulent strains (r. c. macfarlane and u. singh, infect. immun. 74:340-351, 2006). one gene, which we have named ehstirp (entamoeba histolytica serine-, threonine-, and isoleucine-rich protein), was exclusively expressed in virule ... | 2007 | 17827347 |
| differences in protein profiles of the isolates of entamoeba histolytica and e. dispar by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (seldi-tof ms) proteinchip assays. | surface-enhanced laser desorption ionization time of flight mass spectrometry (seldi-tof ms) proteinchip assays with weak cationic exchange chips were used for protein profiling of different isolates of entamoeba histolytica and e. dispar. when seldi-tof ms spectra of cell lysates from e. histolytica strain hm-1:imss were compared with those from four other laboratory strains (200:nih, hk-9, dkb, and saw755cr) grown under the same culture conditions, different peak patterns of seldi-tof ms were ... | 2007 | 17846790 |
| viability studies of pure carbon- and nitrogen-doped nanotubes with entamoeba histolytica: from amoebicidal to biocompatible structures. | 2007 | 17849378 | |
| [a case of amebic brain abscess]. | amebic brain abscess is a rare and usually fatal complication of entamoeba histolytica infections. we successfully treated a patient with this infection accompanied by brain, liver and pulmonary abscesses. treatment consisted of administration of metronidazole through a nasogastric tube, and drainage of the brain abscess. a 51-year-old male patient presented with a two-week history of fever, severe back pain, and chest pain. liver and pulmonary abscesses were drained upon admission. biopsy of th ... | 2007 | 17867313 |
| detection of diarrhoea-causing protozoa in general practice patients in the netherlands by multiplex real-time pcr. | the diagnostic value of a multiplex real-time pcr for the detection of entamoeba histolytica, giardia lamblia and cryptosporidium parvum/cryptosporidium hominis was evaluated by comparing the pcr results obtained with those of routinely performed microscopy of faecal samples from patients consulting their general practitioner (gp) because of gastrointestinal complaints. analysis of 722 faecal dna samples revealed that the prevalence of g. lamblia was 9.3% according to pcr, as compared to 5.7% by ... | 2007 | 17714523 |
| epidemiology of amoebic liver abscess in mexico: the case of sonora. | 2007 | 17716437 | |
| lipopopeptidephosphoglycan from entamoeba histolytica activates human macrophages and dendritic cells and reaches their late endosomes. | lipopopeptidephosphoglycan (lppg) is a complex macromolecule from the surface of entamoeba histolytica trophozoites. we analysed the interaction between lppg and human macrophages and dendritic cells (dcs) and found that lppg is internalized by these cells and activates them. the internalization process involves intracellular traffic from the cell membrane to late endosomes, as shown by co-localization of lppg with late endosomes marked with fitc-dextran and lamp-1. lppg-activated dcs have incre ... | 2007 | 17727570 |
| molecular and functional characterization of ehpak3, a p21 activated kinase from entamoeba histolytica. | p21-activated kinases (paks) are a family of serine/threonine kinases whose activity is regulated by the binding of the small rho family gtpases as well as by rhogtpase independent mechanisms. paks have wide-ranging functions which include cytoskeletal organisation, cell motility, cell proliferation and survival. we have identified a pak from entamoeba histolytica - ehpak3 that is distributed in the cytoplasm of unstimulated cells and localizes to the caps after induction of capping with concana ... | 2007 | 17761392 |
| identification and biochemical characterization of unique secretory nucleases of the human enteric pathogen, entamoeba histolytica. | the ancient eukaryotic human pathogen, entamoeba histolytica, is a nucleo-base auxotroph (i.e. lacks the ability to synthesize purines or pyrimidines de novo) and therefore is totally dependent upon its host for the supply of these essential nutrients. in this study, we identified two unique 28-kda, dithiothreitol-sensitive nucleases and showed that they are constitutively released/secreted by parasites during axenic culture. using several different molecular approaches, we identified and charac ... | 2007 | 17766245 |
| transcriptional gene silencing reveals two distinct groups of entamoeba histolytica gal/galnac-lectin light subunits. | the entamoeba histolytica cell surface gal/galnac-inhibitable lectin is a heterodimer between a heavy (170 kda) subunit linked via a disulfide bond to a light (31 to 35 kda) subunit. five light subunit genes with high homology have been identified (ehlgl1 to -5). we have previously shown that silencing of the expression of ehlgl1, in the g3 trophozoites which had already been silenced in the amoebapore gene (ehap-a), also suppressed the transcription of ehlgl2 and -3 (strain rbv). the total abse ... | 2007 | 17766462 |
| pressure and denaturants in the unfolding of triosephosphate isomerase: the monomeric intermediates of the enzymes from saccharomyces cerevisiae and entamoeba histolytica. | triosephosphate isomerase (tim) is a dimeric enzyme formed by two identical (beta/alpha)8 barrels. in this work, we compare the unfolding and refolding of the tims from entamoeba histolytica (ehtim) and baker's yeast (ytim). a monomeric intermediate was detected in the gdnhcl-induced unfolding of ehtim. the thermodynamic, spectroscopic, catalytic, and hydrodynamic properties of this intermediate were found to be very similar to those previously described for a monomeric intermediate of ytim obse ... | 2007 | 17595057 |
| the evolution of n-glycan-dependent endoplasmic reticulum quality control factors for glycoprotein folding and degradation. | asn-linked glycans (n-glycans) play important roles in the quality control (qc) of glycoprotein folding in the endoplasmic reticulum (er) lumen and in er-associated degradation (erad) of proteins by cytosolic proteasomes. a udp-glc:glycoprotein glucosyltransferase glucosylates n-glycans of misfolded proteins, which are then bound and refolded by calreticulin and/or calnexin in association with a protein disulfide isomerase. alternatively, an alpha-1,2-mannosidase (mns1) and mannosidase-like prot ... | 2007 | 17606910 |
| trichostatin a effects on gene expression in the protozoan parasite entamoeba histolytica. | histone modification regulates chromatin structure and influences gene expression associated with diverse biological functions including cellular differentiation, cancer, maintenance of genome architecture, and pathogen virulence. in entamoeba, a deep-branching eukaryote, short chain fatty acids (scfa) affect histone acetylation and parasite development. additionally, a number of active histone modifying enzymes have been identified in the parasite genome. however, the overall extent of gene reg ... | 2007 | 17612405 |
| intron length distributions and gene prediction. | accurate gene prediction in eukaryotes is a difficult and subtle problem. here we point out a useful feature of expected distributions of spliceosomal intron lengths. since introns are removed from transcripts prior to translation, intron lengths are not expected to respect coding frame, thus the number of genomic introns that are a multiple of three bases ('3n introns') should be similar to the number that are a multiple of three plus one bases (or plus two bases). skewed predicted intron lengt ... | 2007 | 17617639 |
| intranasal immunization with gal-inhibitable lectin plus an adjuvant of cpg oligodeoxynucleotides protects against entamoeba histolytica challenge. | the development of an effective amebiasis vaccine could improve child health in the developing world, reducing cases of amebic colitis and liver abscess. an ideal vaccine would be comprised of a well-characterized parasite antigen and an adjuvant, which would have high potency while driving the immune response in a th1 direction. this study describes a mucosal vaccine composed of the entamoeba histolytica galactose/n-acetyl-d-galactosamine-inhibitable lectin (gal-lectin) and cpg oligodeoxynucleo ... | 2007 | 17620349 |
| the use of the antifungal agent miconazole as an inhibitor of blastocystis hominis growth in entamoeba histolytica/e. dispar cultures. | in regions with high prevalence, blastocystis hominis is frequently found in association with entamoeba histolytica/e. dispar in xenic cultures. its exacerbated growth is often superimposed on the growth of amebas, thus impeding the continuation of the amebas in the culture, within a few generations. the present study reports on the excellent efficacy (100%) of the antifungal agent miconazole in eliminating b. hominis from cultures of e. histolytica/e. dispar, thereby maintaining the integrity o ... | 2007 | 17625701 |
| ehlima, a novel lim protein, localizes to the plasma membrane in entamoeba histolytica. | the parasitic protozoan entamoeba histolytica relies on a very dynamic cytoskeleton in order to invade and survive in host tissues. identification of cytoskeletal elements is key to understanding these processes. here we present the characterization of ehlima, the first lim protein of e. histolytica. ehlima consists of a single lim domain at its n terminus and exhibits the highest degree of homology with ddlime from dictyostelium discoideum. immunofluorescence localization of ehlima using anti-e ... | 2007 | 17630327 |
| laboratory diagnostic techniques for entamoeba species. | the genus entamoeba contains many species, six of which (entamoeba histolytica, entamoeba dispar, entamoeba moshkovskii, entamoeba polecki, entamoeba coli, and entamoeba hartmanni) reside in the human intestinal lumen. entamoeba histolytica is the causative agent of amebiasis and is considered a leading parasitic cause of death worldwide in humans. although recent studies highlight the recovery of e. dispar and e. moshkovskii from patients with gastrointestinal symptoms, there is still no convin ... | 2007 | 17630338 |
| diarrhoea-associated parasites in hiv-1 seropositive and sero-negative patients in a teaching hospital, northwest ethiopia. | to determine the prevalence and type of intestinal parasites in hiv infected and uninfected patients with diarrhea. | 2007 | 17642173 |
| diarrhoeagenic escherichia coli and other causes of childhood diarrhoea: a case-control study in children living in a wastewater-use area in hanoi, vietnam. | a case-control study was conducted to identify the aetiology of diarrhoeal diseases in pre-school children in a suburban area of hanoi where the use of untreated wastewater in agriculture and aquaculture is a common practice. stool specimens and clinical information were collected from 111 pairs of children with diarrhoea and healthy controls. a total of 73 cases (66 %) and 41 controls (36 %) had an enteric pathogen. the pathogens most often associated with diarrhoea were rotavirus (17 % of case ... | 2007 | 17644717 |
| detection of cryptosporidium spp and other intestinal parasites in children with acute diarrhea and severe dehydration in rio de janeiro. | the objective of the present study was to estimate the frequency of infection by cryptosporidium spp and other intestinal parasites in dehydrated children with gastroenteritis who were admitted to a pediatric hospital. stool examinations from 218 children were performed. cryptosporidium spp was identified in eighteen out of 193 stool samples (9.3%) subjected to safranin-methylene blue staining. giardia lamblia was detected in ten out of 213 (4.7%) samples examined via the direct or ritchie metho ... | 2007 | 17653475 |
| non-biting cyclorrhaphan flies (diptera) as carriers of intestinal human parasites in slum areas of addis ababa, ethiopia. | a study was conducted to determine the role of non-biting cyclorrhaphan flies as carriers of intestinal parasites in slum areas of addis ababa from january 2004 to june 2004. a total of 9550 flies, comprising of at least seven species were collected from four selected sites and examined for human intestinal parasites using the formol-ether concentration method. the dominant fly species was chrysomya rufifacies (34.9%) followed by musca domestica (31%), musca sorbens (20.5.%), lucina cuprina (6.8 ... | 2007 | 17658447 |
| nitazoxanide in the treatment of amoebiasis. | amoebiasis is a significant cause of morbidity worldwide and is the third leading cause of death from parasitic diseases. this study evaluated nitazoxanide, a thiazolide anti-infective, in the treatment of intestinal and hepatic amoebiasis. prospective, randomised, double-blind, placebo-controlled studies were conducted in outpatients with intestinal amoebiasis from the nile delta of egypt. nitazoxanide was administered twice daily for 3 days at doses of 500mg (age > or =12 years), 200mg (age 4- ... | 2007 | 17658567 |
| [confirmation by pcr of the existence in tunisia of entamoeba histolytica and entamoeba dispar]. | amibias are illness in tunisia diagnosed until now on the sole basis of the morphological aspects of the parasite. our aim is to report the first tunisian results concerning the molecular identification of e. histolytica/e. dispar, | 2007 | 17668576 |
| computational study of glyceraldehyde-3-phosphate dehydrogenase of entamoeba histolytica: implications for structure-based drug design. | glyceraldehyde-3-phosphate dehydrogenase (gapdh) of the pathogenic protozoa entamoeba histolytica (eh) is a major glycolytic enzyme and an attractive drug target since this parasite lacks a functional citric acid cycle and is dependent solely on glycolysis for its energy requirements. the three-dimensional structure of dimeric ehgapdh in complex with cofactor nad(+) has been generated by homology modeling based on the crystal structure of human liver gapdh. our refined model indicates the presen ... | 2007 | 17676935 |
| nitroimidazole action in entamoeba histolytica: a central role for thioredoxin reductase. | metronidazole, a 5-nitroimidazole drug, has been the gold standard for several decades in the treatment of infections with microaerophilic protist parasites, including entamoeba histolytica. for activation, the drug must be chemically reduced, but little is known about the targets of the active metabolites. applying two-dimensional gel electrophoresis and mass spectrometry, we searched for protein targets in e. histolytica. of all proteins visualized, only five were found to form adducts with me ... | 2007 | 17676992 |
| comparison of multiplex-pcr and antigen detection for differential diagnosis of entamoeba histolytica. | amebiasis is an infection caused by entamoeba histolytica. however, differentiation between e. histolytica and entamoeba dispar, which are morphologically identical species, is essential for treatment decision, precaution of the invasive disease and public health. the purpose of the present study was to evaluate a multiplex -pcr for detection and differentiation of e. histolytica from e. dispar from fresh stool samples in comparison with the coproantigen commercial elisa. microscopic examination ... | 2007 | 17684641 |
| energetics of the native energy landscape of a two-domain calcium sensor protein: distinct folding features of the two domains. | the protein folding energy landscape allows a thorough understanding of the protein folding problem which in turn helps in understanding various aspects of biological functions. characterizing the cooperative unfolding units and the intermediates along the folding funnel of a protein is a challenging task. in this paper, we investigated the native energy landscape of ehcabp, a calcium sensor, belonging to the same ef-hand superfamily as calmodulin. ehcabp is a two-domain ef-hand protein consisti ... | 2007 | 17685557 |
| [autochthonous amebic liver abscess: microbiological diagnosis by pcr]. | 2007 | 17692221 | |
| diagnosis of intestinal amoebiasis by using nested polymerase chain reaction-restriction fragment length polymorphism assay. | microscopy is unreliable to distinguish the pathogenic entamoeba histolytica from the nonpathogenic entamoeba dispar or entamoeba moshkovskii in stool specimens. | 2007 | 17701126 |
| crosstalk at the initial encounter: interplay between host defense and ameba survival strategies. | the host-parasite relationship is based on a series of interplays between host defense mechanisms and parasite survival strategies. progress has been made in understanding the role of host immune response in amebiasis. while host cells elaborate diverse mechanisms for pathogen expulsion, amebae have also developed complex strategies to modulate host immune response and facilitate their own survival. this paper will give an overview of current research on the mutual interactions between host and ... | 2007 | 17702556 |
| novel structural and functional findings of the ehfln protein from entamoeba histolytica. | the ehfln protein (previously known as ehabp-120) is the first filamin to be identified in the parasitic protozoan entamoeba histolytica. filamins are a family of cross-linking actin-binding proteins that organize filamentous actin in networks and stress fibers. it has been reported that filamins of different organisms directly interact with more than 30 cellular proteins and some ppis. the biochemical consequences of such interactions may have either positive or negative effects on the cross-li ... | 2007 | 17705278 |
| functional characterization of spliceosomal introns and identification of u2, u4, and u5 snrnas in the deep-branching eukaryote entamoeba histolytica. | pre-mrna splicing is essential to ensure accurate expression of many genes in eukaryotic organisms. in entamoeba histolytica, a deep-branching eukaryote, approximately 30% of the annotated genes are predicted to contain introns; however, the accuracy of these predictions has not been tested. in this study, we mined an expressed sequence tag (est) library representing 7% of amoebic genes and found evidence supporting splicing of 60% of the testable intron predictions, the majority of which contai ... | 2007 | 17468393 |