Publications

preparation, characterization, and reconstitution of oxaloacetate decarboxylase from klebsiella aerogenes, a sodium pump.		1986	3520232
structural and kinetic studies on beta-lactamase k1 from klebsiella aerogenes.	beta-lactamase k1 from klebsiella aerogenes 1082e hydrolyses both penicillins and cephalosporins comparably and is inhibited by mercurials but not by cloxacillin. these properties distinguish it from those other beta-lactamases that have been allotted to classes on the basis of their amino sequences. beta-lactamase k1 has been isolated by affinity chromatography; its composition shows resemblances to class a beta-lactamases. moreover, the n-terminal sequence is similar to those of class a beta-l ...	1986	3521585
bacterial antagonists of shigellae.	bacterial antagonism between a microorganisms and shigella sonnei strains was studied in model experiments simulating conditions of the natural aquatic environment. in these studies surface waste samples from the river vltava served as the experimental environment. to ensure bacteriologically defined conditions all water samples were heat-sterilized prior to antagonism testing. consistently with the literature data and author's own observations the following bacterial species and genera were cho ...	1986	3522730
affinity of cephalosporins for beta-lactamases as a factor in antibacterial efficacy.	strains of escherichia coli, enterobacter aerogenes, and enterobacter cloacae that were resistant to ceftazidime (mic greater than 16 micrograms/ml) but susceptible to bmy 28142 (mic less than 4 micrograms/ml) were found to contain higher levels of beta-lactamase activity (50- to 3,340-fold) than control strains of the corresponding species. ceftazidime was at least as resistant as bmy 28142 to hydrolysis by these enzymes. however, the apparent ki of bmy 28142 for each enzyme was larger (8- to g ...	1986	3524432
r plasmids, kr61 and kr61-a, from aerobacter aerogenes.		1986	3525399
[effect of cationic polymers and surface-active substances on bacterial resistance to kanamycin].	the effect of cationic polymers and surface active substances (sas) on sensitivity of klebsiella aerogenes 600, escherichia coli 154 and proteus vulgaris 7470 to kanamycin was studied. a decrease in the resistance of the above organisms to kanamycin on its use in combination with cationic polymers and sas was observed. it was shown that such substances inhibited the activity of the enzymes inactivating kanamycin. their effect was suggested to be due to changes in the lipid surrounding of the enz ...	1986	3527060
magnesium-dependent plaque formation by bacteriophage p1cinc(-) on escherichia coli c and shigella sonnei.	phage p1c(-), in a state of the phage not infective to escherichia coli k12, was able to form plaques on a wild-type strain of e. coli c and on shigella sonnei in the presence of mg2+. citrobacter freundii, enterobacter aerogenes, and a salmonella typhimurium gale mutant were not lysed by, but were lysogenized with p1cinc(-), whereas klebsiella pneumoniae, proteus rettgeri, and s. typhimurium lt2 were not susceptible to either p1cinc(-) or p1cinc(+). the lipopolysaccharide structure of e. coli c ...	1986	3535235
bacteria and helminth isolates from bile and faeces of zebu cattle slaughtered for human consumption in the niger delta areas of nigeria.	random bacteriological examination of 300 bile samples aseptically drawn from the gallbladders of adult cattle at slaughter revealed the presence of escherichia coli in 30%, enterobacter aerogenes in 14% and klebsiella aerogenes in 56%. bacteriological examination of another 116 faecal samples revealed esch. coli in 60% and ent. aerogenes in 40%, while parasitological examination revealed 50-100 strongyle ova per gram from pre-rain samples of faeces, 100-850 strongyle eggs per gram for faeces co ...	1986	3539044
isolation and characterization of the m.eaei modification methylase.	the modification enzyme (m.eaei) corresponding to the restriction endonuclease eaei was partially purified from enterobacter aerogenes pw201. the m.eaei enzyme methylates the innermost cytosine residue in each strand of the family of related sequences that constitute the eaei recognition site to give: 5'-y-g-g-5mc-c-r-3' where 5mc is 5-methylcytosine. m.eaei protects these sites against cleavage by haeiii, and protects overlapping 5'-c-c-g-g-3' sites against cleavage by both hpaii and mspi.	1986	3467722
antimicrobial activity of imipenem against 1386 clinical isolates.	1386 isolates from clinical specimens were tested against imipenem by disc agar diffusion. the bacteria used in this study consisted of escherichia coli, enterobacter aerogenes, e. agglomerans, e. cloacae, klebsiella pneumoniae, k. oxytoca, k. ozanae, proteus mirabilis, p. vulgaris, providencia stuartii, p. rettgeri, acinetobacter calcoaceticus, citrobacter diversus, c. freundii, morganella morganii, serratia liquefaciens, s. marcescens, hafnia alvei, aeromonas hydrophila, pseudomonas aeruginosa ...	1986	3468319
efficacy of imipenem/cilastatin in endocarditis.	imipenem, a potent new beta-lactam antibiotic, which is bactericidal against most pathogenic bacteria, and cilastatin, a dehydropeptidase inhibitor combined with imipenem to prevent the metabolism of imipenem in the kidney, were evaluated in the treatment of bacterial endocarditis. seventeen patients, including 14 who used intravenous drugs, were treated with imipenem/cilastatin in a dose of 500 mg each infused over 30 minutes every six hours. the mean duration of treatment was 29 days with a ra ...	1985	3859210
enoxacin compared with cefoperazone for the treatment of experimental enterobacter aerogenes endocarditis.	this study compared enoxacin administered orally with cefoperazone administered intramuscularly for the treatment of enterobacter aerogenes endocarditis in rabbits. the mics and mbcs of both enoxacin and cefoperazone for an inoculum of 10(5) cfu/ml of the e. aerogenes strain used were 0.8 micrograms/ml, respectively. with an inoculum of 10(8) organisms per ml, enoxacin at 2 and 5 micrograms/ml and cefoperazone at 60 and 155 micrograms/ml were effective in reducing titers of e. aerogenes in broth ...	1985	3860186
influence of imipenem on the serum resistance of enterobacteriaceae.	following growth in a subinhibitory concentration of imipenem and additional incubation in a 20% dilution of normal human serum (nhs) for 90 minutes, five of 12 serum-resistant strains of enterobacteriaceae showed a decrease in colony-forming units of two or more logs of growth compared with the control. two strains (of escherichia coli and enterobacter aerogenes) showed this phenomenon even with incubation in 5% nhs. treatment with imipenem did not change the serum resistance of the other seven ...	1985	3863217
the activity of cefbuperazone, a 7 alpha-methoxy 7 beta acyl ureido cephalosporin.	the activity of cefbuperazone, a 7 alpha-methoxy ureido cephalosporin, was determined against 726 clinical isolates. ninety percent of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, citrobacter diversus, proteus mirabilis, enterobacter aerogenes, proteus vulgaris, morganella morganii, salmonella, and shigella species were inhibited by less than or equal to 6.3 micrograms/ml. cefbuperazone was more active than cefamandole, cefoxitin and piperacillin against these species. concentrat ...	1985	3879464
in vivo synthesis of histidine by a cloned histidine ammonia-lyase in escherichia coli.	histidine ammonia-lyase catalyzes the first step in histidine catabolism, the deamination of histidine to urocanate and ammonia. in vitro experiments have shown that histidine ammonia-lyase also can catalyze the reverse (amination) reaction, histidine synthesis, relatively efficiently under extreme reaction conditions (4 m nh4oh, ph 10). an escherichia coli hisb deletion strain was transformed with a pbr322 derivative plasmid (pcb101) containing the entire klebsiella aerogenes histidine utilizat ...	1985	3884601
cloning of the pullulanase gene and overproduction of pullulanase in escherichia coli and klebsiella aerogenes.	the pullulanase gene (pul) of klebsiella aerogenes was cloned into a pbr322 vector in escherichia coli. deletion analysis of the recombinant plasmid showed that the pul coding sequence, probably with the regulator gene, was located entirely within a 4.2-kilobase segment derived from the chromosomal dna of k. aerogenes. e. coli cells carrying the recombinant plasmids produced about three- to sevenfold more pullulanase than did the wild-type strain of k. aerogenes w70. when the cloned cells of e. ...	1985	3885852
in vitro studies on the antibacterial activities of ym-13115, a new broad-spectrum cephalosporin.	the in vitro antibacterial activities of ym-13115, a new parenteral cephalosporin, were compared with those of ceftazidime, cefoperazone, and cefsulodin. the compound was highly active against the common members of the enterobacteriaceae and 2 to 256 times more active than cefoperazone. ym-13115 was as active as ceftazidime against citrobacter freundii, proteus vulgaris, and morganella morganii and two to four times more active than ceftazidime against escherichia coli, klebsiella pneumoniae, en ...	1985	3890729
structure of wild-type and mutant repressors and of the control region of the rbt operon of klebsiella aerogenes.	pentitol metabolism in klebsiella aerogenes is encoded by continuous ribitol (rbt) and d-arabitol (dal) operons transcribed in bipolar fashion and sandwiched between long stretches of homologous dna. the operons are separated by a central control region (2.2 kb) which encodes both the repressors and all the control sequences. the rbt repressor (270 amino acids) shows homology to the escherichia coli lac repressor and other dna-binding proteins. it is transcribed from the strand opposite the rbt ...	1985	3891331
evolution of glutamine amidotransferase genes. nucleotide sequences of the paba genes from salmonella typhimurium, klebsiella aerogenes and serratia marcescens.	the amide group of glutamine is a source of nitrogen in the biosynthesis of a variety of compounds. these reactions are catalyzed by a group of enzymes known as glutamine amidotransferases; two of these, the glutamine amidotransferase subunits of p-aminobenzoate synthase and anthranilate synthase have been studied in detail and have been shown to be structurally and functionally related. in some micro-organisms, p-aminobenzoate synthase and anthranilate synthase share a common glutamine amidotra ...	1985	3894673
comparison of the regulatory regions of ilvgeda operons from several enteric organisms.	the nucleotide sequence preceding the ilvgeda operon has been examined and compared in five enteric organisms. the sequence in escherichia coli b was identical to the earlier-described strain k-12 sequence. the sequences of salmonella typhimurium and klebsiella aerogenes were remarkably similar to that of e. coli and identical in that part of the leader region that specified the putative 32-amino-acid peptide. thus, identical secondary structures could be postulated for the leaders of all three ...	1985	3900037
microcalorimetry studies of energy changes during the growth of klebsiella aerogenes in simple salts/glucose media; correlation of specific power and size of the atp pool.	a parallel study has been made of the variation of the atp pool and the specific power of cells of klebsiella aerogenes during aerobic growth in glucose-limited medium under carefully defined conditions of growth and test. during the early part of exponential growth there was a marked increase in both the atp pool and the specific power to near constant values during the later stages of growth; oscillations about the mean values were observed for each parameter. with the exhaustion of glucose an ...	1985	3903440
ribitol dehydrogenase of klebsiella aerogenes. sequence and properties of wild-type and mutant strains.	evidence is presented for the sequence of 249 amino acids in ribitol dehydrogenase-a from klebsiella aerogenes. continuous culture on xylitol yields strains that superproduce 'wild-type' enzyme but mutations appear to have arisen in this process. other strains selected by such continuous culture produce enzymes with increased specific activity for xylitol but without loss of ribitol activity. one such enzyme, ribitol dehydrogenase-d, has pro-196 for gly-196. another, ribitol dehydrogenase-b, has ...	1985	3904726
interspecific reconstitution of maltose transport and chemotaxis in escherichia coli with maltose-binding protein from various enteric bacteria.	in escherichia coli, the periplasmic maltose-binding protein (mbp), the product of the male gene, is the primary recognition component of the transport system for maltose and maltodextrins. it is also the maltose chemoreceptor, in which capacity it interacts with the signal transducer tar (taxis to aspartate and some repellents). in studies of the maltose system in other members of the family enterobacteriaceae, we found that mbp is produced by salmonella typhimurium, klebsiella pneumoniae, ente ...	1985	3905762
the functional significance of glucose dehydrogenase in klebsiella aerogenes.	in order to assess the functional significance of the quinoprotein glucose dehydrogenase recently found to be present in k+ -limited klebsiella aerogenes, a broad study was made of the influence of specific environmental conditions on the cellular content of this enzyme. whereas high activities were manifest in cells from glucose containing chemostat cultures that were either potassium- or phosphate-limited, only low activities were apparent in cells from similar cultures that were either glucos ...	1985	3907571
glutamate synthase from escherichia coli, klebsiella aerogenes, and saccharomyces cerevisiae.		1985	3911003
detoxification of mercury, cadmium, and lead in klebsiella aerogenes nctc 418 growing in continuous culture.	klebsiella aerogenes nctc 418 growing in the presence of cadmium under glucose-, sulfate-, or phosphate-limited conditions in continuous culture exhibited sulfide formation and pi accumulation as the only demonstrable detoxification mechanisms. in the presence of mercury under similar conditions only hgs formation could be confirmed, by an increased sensitivity to mercury under sulfate-limited conditions, among others. the fact that the cells were most sensitive to cadmium under conditions of ph ...	1985	3911898
microcalorimetry studies of energy changes during the growth of klebsiella aerogenes in simple salts/glucose media: growth in phosphate-limited medium.	the power-time traces for cells of klebsiella aerogenes grown in phosphate-limited media were unlike those for cells grown in phosphate-sufficient media. at a phosphate concentration of 18 mumol dm-3 three phases of growth were recognized: the exponential growth phase during which phosphate became exhausted; a slower growth phase to the exhaustion of glucose, and a period of minimal growth when acetate, formed as secondary metabolite, was metabolized. at a concentration of 40 mumol dm-3 only two ...	1985	3913845
growth of microorganisms in total parenteral nutrition mixtures and related clinical observations.	the effect of lipid addition to tpn (total parenteral nutrition) solutions on microbial growth was investigated. staphylococcus epidermidis, which failed to grow or grew poorly in the absence of lipid, reached greater than 10(4) cfu/ml (colony forming units per ml), from an initial inoculum of approximately 50 cfu/ml after 24 h when lipid was added. candida albicans grew more slowly in the presence of lipid, but nevertheless reached 10(4) cfu/ml after 40 h incubation. klebsiella aerogenes grew r ...	1985	3923044
growth of bacteria in enteral feeding solutions.	solutions of clinifeed iso, triosorbon, vivonex standard (full- and half-strength) and vivonex hn were experimentally contaminated with two strains each of staphylococcus aureus, pseudomonas aeruginosa, klebsiella aerogenes, escherichia coli and enterobacter cloacae at concentrations of 10(2)-10(3) organisms/ml. samples were incubated at 4, 25 or 37 degrees c and viable counts were made at 0, 4, 8 and 24 h. no increase in numbers of any of the organisms was observed in any of the feeds during 24 ...	1985	3927003
measuring the spermosphere colonizing capacity (spermosphere competence) of bacterial inoculants.	spermosphere establishment by bacteria which were coated onto seeds was studied using soybean seeds treated with four bacterial strains at levels of log10 1 to 4 colony-forming units (cfu) per seed planted in a field soil mix, and incubated 48 h. each strain at every inoculum level developed spermosphere population densities of log10 4 to 8 cfu/seed, demonstrating an average multiplication of log10 3 cfu/seed. an alternative method was developed to differentially rank bacteria for spermosphere c ...	1985	3933804
ofloxacin: in-vitro activity against clinical isolates.	the in-vitro activity of ofloxacin, a new quinolone derivative, was evaluated in 273 bacterial strains isolated from urine. the results showed a remarkable activity against most gram-negative bacteria and, in particular, against enterobacter aerogenes, which is very difficult to eradicate even with third-generation cephalosporins.	1985	3938347
microcalorimetry studies of energy changes during the growth of klebsiella aerogenes in simple salts/glucose media: inhibition by nalidixic acid.	the addition of nalidixic acid (na) to exponentially growing cells of klebsiella aerogenes in simple salts/glucose media has a marked effect on the thermal and other growth parameters of the cells. mass and energy balances have been established for normal and inhibited growth. the decrease, with increasing na concentration, of energy allocated to biomass and the increase in that wasted is discussed in terms of the energy stored in macromolecular species by the cells under conditions of stress. t ...	1985	3938832
phenotypic variations in cell envelope proteins of klebsiella aerogenes.	the structure and functioning of the cell envelope of klebsiella aerogenes vary phenotypically with changes in the culture environment. these variations involve, in particular, the proteinaceous components. this contribution discusses results obtained in recent studies directed towards analysing the influence of growth conditions on membrane protein composition in this organism.	1985	3940006
dynamics of resistance to cephalosporins.	bacterial susceptibility to various cephalosporins of nine of the most frequently isolated organisms were analyzed for the five-year period 1980 to 1984. the disk diffusion susceptibility to cephalosporins of 1,640,031 strains of bacteria isolated from 242 hospitals in the united states were compared. no significant change has occurred in the susceptibility of escherichia coli, klebsiella pneumoniae, proteus mirabilis, haemophilus influenzae, or streptococcus faecalis for this period; enterobact ...	1985	3986942
the emergence of resistance and the therapy of septicaemia.	there were 658 positive blood cultures in st. thomas' hospital during 1982-1984, from which 301 enterobacteriaceae were isolated. most were of species that do not usually produce inducible class i beta-lactamases but 39 of the isolates were of species that do. many of these strains were resistant to the older cephalosporins but resistance to cefuroxime was uncommon and resistance to ceftazidime or cefotaxime was confined to five isolates of enterobacter cloacae (four of which were from patients ...	1985	3986947
mineral nutrition of aerobacter aerogenes for valine production in a synthetic medium.	the effect of a number of mineral salts, like dipotassium hydrogen phosphate, magnesium sulphate, and sodium chloride, and of some trace elements including iron, copper, cobalt, nickel, molybdenum, and calcium, on the production of valine by aerobacter aerogenes in a synthetic medium was investigated. it was found that all the mineral salts were necessary for valine formation. among the trace elements, iron and molybdenum were found to be necessary in minute concentrations for the optimum yield ...	1985	4013530
biochemical changes during valine production by aerobacter aerogenes.	the typical chemical changes taking place during valine production by a. aerogenes in a synthetic medium have been investigated. the principal carbon and nitrogen sources, sucrose and urea, respectively, have been found to be almost exhausted during an incubation period lasting 90 h. while the carbon compound was depleted right from the start of formentation, the disappearance of urea was noticeable after 16 h of incubation. the formation of valine in the broth started between 24 and 40 h of inc ...	1985	4013531
crepitant cellulitis due to enterobacter aerogenes.		1985	4019153
activation of herpes simplex following dermabrasion. report of a patient successfully treated with intravenous acyclovir and brief review of the literature.	herpes simplex labialis developed in a patient immediately following dermabrasion. the patient was hospitalized because the infection had spread rapidly over the dermabraded face and was complicated by secondary impetiginization. herpesvirus hominis type i and enterobacter aerogenes were isolated from cultures. intravenous acyclovir and oral antibiotics were administered. on this regimen, new vesicle formation ceased in 36 hours. complete resolution of the infection occurred within 6 days, with ...	1985	4031143
treatment of gram-negative infections with aztreonam.	twenty-one patients with serious gram-negative infections were treated with aztreonam. twenty of these were clinical and microbiologic cures; there was one clinical improvement with microbiologic persistence. no bacteria became resistant. cure rates were: bone and joint (11 of 11); skin and soft tissue (six of six); pneumonia (two of two); perinephric abscess (one of one); and intra-abdominal abscess (zero of one). the bacteria responsible for these infections included pseudomonas aeruginosa (12 ...	1985	4038577
efficacy and safety of aztreonam-clindamycin versus tobramycin-clindamycin in the treatment of lower respiratory tract infections caused by aerobic gram-negative bacilli.	a total of 80 patients were randomized to receive either aztreonam or tobramycin for the treatment of lower respiratory tract infections caused by gram-negative bacilli; all these patients received clindamycin concomitantly. a total of 53 patients were randomized to receive aztreonam-clindamycin; of these, 46 were clinically evaluable and 39 were bacteriologically evaluable. of the 46 clinically evaluable patients, 41 were considered cured, 3 failed to be cured, and 2 died during the study perio ...	1985	4039118
liver abscess complicating crohn's disease presenting as thoracic empyema. case report and review of the literature.	a 28-year-old man with a history of crohn's disease presented with right pleuritic pain and dyspnea. chest radiography showed a right pleural effusion. thoracocentesis yielded purulent fluid that subsequently grew enterobacter aerogenes. computed axial tomography of the abdomen revealed right subphrenic abscess and right hepatic lobe abscess. antibiotic therapy and surgical drainage resulted in complete recovery. a review of the english literature produced 18 cases of liver abscess complicating ...	1985	4061480
a preliminary survey of aerobic bacteria in breast milk of mothers from the low-income group in nigeria.	the aerobic bacteria colonizing breast milk of the low-income group in nigeria were quantified to assess its suitability for use in milk banks. in parallel, the nutritional and health status of donating mothers and their infants were assessed by physicians. the aerobic bacteria contained in the specimens included streptococcus salivarius, bacillus cereus, klebsiella pneumoniae, enterobacter aerogenes, streptococcus pneumoniae, streptococcus pyogenes, pseudomonas aeruginosa and staphylococcus epi ...	1985	4071647
bacterial isolates from nsukka meat market: a zoonotic appraisal.	swabs collected from butchers hands, knives, tables and meat displayed for sale at nsukka meat market showed that every item screened was contaminated. bacteria isolated include streptococcus faecalis, coagulase positive staphylococcus, clostridium perfringens, brucella species, corynebacterium haemolyticum, proteus mirabilis, p. vulgaris, escherichia coli, salmonella enteritidis, enterobacter aerogenes, pseudomonas multophilia, p. rubescens and beta-haemolytic streptococci. the zoonotic or publ ...	1985	4077409
the klebsiella aerogenes glutamate dehydrogenase (gdha) gene: cloning, high-level expression and hybrid enzyme formation in escherichia coli.	the nadp-dependent glutamate dehydrogenase gene of klebsiella aerogenes was cloned in e. coli in the expression plasmid prk9. the cloned gene shows a high level of expression in e. coli in the hybrid plasmid pkg3 and such expression is independent of the vector promoter, as shown by experiments in which the promoter was deleted. active hybrid gdh hexamers were shown in cell-free extracts of an e. coli strain carrying cloned gdha genes of both e. coli and k. aerogenes. the nucleotide sequence of ...	1985	2987645
ribitol dehydrogenase of klebsiella aerogenes. sequence of the structural gene.	the ribitol dehydrogenase gene was cloned from wild-type klebsiella aerogenes and also from a transducing phage lambda prbt which expresses the rbt operon constitutively. the coding sequence for 249 amino acids is separated from the following d-ribulokinase gene by 31 base pairs containing three stop codons, one of which overlaps the ribosome binding site for d-ribulokinase. three residues in the amino acid sequence differ from that predicted from the dna sequence: asp-212 for asn-212 is probabl ...	1985	2933028
aztreonam in the treatment of bone and joint infections caused by gram-negative bacilli.	aztreonam was used successfully in 17 of 17 patients with orthopedic infections due to gram-negative bacilli (11, osteomyelitis; six, septic arthritis). duration of treatment ranged from 14 to 55 days, and the period of follow-up was four to 18 months. causative organisms included pseudomonas aeruginosa, serratia marcescens, enterobacter gergoviae, citrobacter diversus, proteus mirabilis, and enterobacter aerogenes. aztreonam was well tolerated. the only definite reactions attributable to aztreo ...	1985	2934786
fimbrial and non-fimbrial haemagglutinins in enterobacter aerogenes.	ten strains of enterobacter aerogenes were examined for their ability to produce haemagglutinins and fimbriae. nine strains formed a mannose-sensitive (ms) haemagglutinin associated with thin (4 nm) non-channelled fimbriae. these thin fimbriae of e. aerogenes were antigenically different from the thin fimbriae of other fimbriate strains of enterobacter and klebsiella and probably represent a new kind of fimbria not previously described in enterobacteriaceae. eight of these same nine strains also ...	1985	2857201
regulation of glutamine synthetase, aspartokinase, and total protein turnover in klebsiella aerogenes.	when suspensions of klebsiella aerogenes are incubated in a nitrogen-free medium there is a gradual decrease in the levels of acid-precipitable protein and of aspartokinase iii (lysine-sensitive) and aspartokinase i (threonine-sensitive) activities. in contrast, the level of glutamine synthetase increases slightly and then remains constant. under these conditions, the glutamine synthetase and other proteins continue to be synthesized as judged by the incorporation of [14c]leucine into the acid-p ...	1985	2865982
cloning and expression of the thiobacillus ferrooxidans glutamine synthetase gene in escherichia coli.	the glutamine synthetase (gs) gene glna of thiobacillus ferrooxidans was cloned on recombinant plasmid pmeb100 which enabled escherichia coli glna deletion mutants to utilize (nh4)2so4 as the sole source of nitrogen. high levels of gs-specific activity were obtained in the e. coli glna deletion mutants containing the t. ferrooxidans gs gene. the cloned t. ferrooxidans dna fragment containing the glna gene activated histidase activity in an e. coli glna glnl glng deletion mutant containing the kl ...	1985	2866177
microbial penetration through nutrient-saturated berea sandstone.	penetration times and penetration rates for a motile bacillus strain growing in nutrient-saturated berea sandstone cores were determined. the rate of penetration was essentially independent of permeabilities above 100 mdarcys and rapidly declined for permeabilities below 100 mdarcys. it was found that these penetration rates could be grouped into two statistically distinct classes consisting of rates for permeabilities above 100 mdarcys and rates for those below 100 mdarcys. instantaneous penetr ...	1985	16346858
nitrogen-fixing bacteria from warty lenticellate bark of a mangrove tree, bruguiera gymnorrhiza (l.) lamk.	detached warty lenticellate bark of a mangrove tree species, bruguiera gymnorrhiza (l.) lamk. from iriomote island, okinawa, a subtropical region of japan, showed development of acetylene reduction activity when incubated in a mineral nutrient solution lacking nitrogen under an atmosphere consisting of 5% o(2), 90% n(2), and 5% c(2)h(2). the bacteria responsible for nitrogen fixation were isolated from the bark, and their capacity for acetylene reduction and the incorporation of n(2) into the ba ...	1984	16346458
butanediol production by aerobacter aerogenes nrrl b199: effects of initial substrate concentration and aeration agitation.	butanediol production by aerobacter aerogenes nrrl b199 grown on glucose requires an optimal rate of aeration for the obtention of butanediol 2, 3. in the absence of air, aerobacter aerogenes nrrl b199 growth and production are weak. agitation-aeration is necessary for producing the biomass, but an excess of oxygen proves to be toxic with regard to metabolite production. oxygen is a limiting substrate with regard to growth and an inhibitor with regard to the specific metabolite productivity. thi ...	1984	18551701
inorganic phosphate accumulation and cadmium detoxification in klebsiella aerogenes nctc 418 growing in continuous culture.	klebsiella aerogenes nctc 418, growing in the presence of cadmium under glucose-, sulfate-, or phosphate-limited conditions in continuous culture, exhibits two different cadmium detoxifying mechanisms. in addition to sulfide formation, increased accumulation of pi is demonstrated as a novel mechanism. intracellular cadmium is always quantitatively counterbalanced by a concerted increase in both inorganic sulfide and pi contents of the cells. this led to the conclusion that production of sulfide ...	1984	6370136
effect of iron deprivation on the production of siderophores and outer membrane proteins in klebsiella aerogenes.	the outer membrane (om) protein profile of klebsiella aerogenes grown in an iron rich chemically defined medium (fe + cdm) showed three major proteins of 32.5, 35.5 and 39.0 kdal. the 35.5 and 39.0 kdal proteins were non-covalently associated with peptidoglycan. at least six new iron regulated outer membrane proteins (irmp) of 69, 70, 73, 75, 78 and 83 kdal, which were not peptidoglycan associated, were apparent in the om of k. aerogenes grown in iron restricted (serum) or iron deficient (fe-cdm ...	1984	6239009
in vitro activity of pefloxacin compared to that of quinolones and other antimicrobial agents.	pefloxacin is a new methyl-4-piperazinyl quinolone. it had mic90 values of less than 0.01 to 0.8 micrograms/ml for the majority of escherichia coli, klebsiella, oxytoca, citrobacter, providencia, enterobacter cloacae, enterobacter aerogenes, morganella and proteus mirabilis. it inhibited ampicillin, cephalexin and nalidixic acid resistant isolates of these species. against pseudomonas the pefloxacin mic90 was 3.1 micrograms/ml. staphylococcus aureus had a mic50 of 0.4 micrograms/ml and a mic90 o ...	1984	6241847
a restriction enzyme cleavage map of the histidine utilization (hut) genes of klebsiella aerogenes and deletions lacking regions of hut dna.	the histidine utilization (hut) operons of klebsiella aerogenes were cloned into pbr322. the hut genes are wholly contained on a 7.9 kilobase pair fragment bounded by hindiii restriction sites and expression of hut is independent of the orientation of the fragment with respect to pbr322. a restriction map locating the 27 cleavage sites within hut for the enzymes, hindiii, pvuii, sali, bglii, kpni, psti, smai, avai, and bamhi was deduced. several of the cleavage sites for the enzymes haeiii and h ...	1984	6318054
5-enolpyruvylshikimate-3-phosphate synthase of klebsiella pneumoniae. 1. purification and properties.	the shikimate pathway enzyme 5-enolpyruvylshikimate-3-phosphate (epsp) synthase (3-phosphoshikimate 1-carboxyvinyltransferase, ec 2.5.1.19) has been purified to apparent homogeneity from aerobacter aerogenes, strain 62-1 (= klebsiella pneumoniae atcc 25306). a 3300-fold purification of the enzyme was achieved by ammonium sulfate fractionation, heat precipitation, chromatography on deae-cellulose, sephadex g-75, and cellulose phosphate, and chromatofocusing as the final step. the recovery was 49% ...	1984	6381056
intergeneric homology of the spec gene encoding biosynthetic ornithine decarboxylase in escherichia coli.	a 32p-labeled fragment of dna containing the spec gene, which encodes the biosynthetic enzyme ornithine decarboxylase of escherichia coli, was used as a hybridization probe for homologous sequences in the genomes of gram-negative and gram-positive bacteria. the spec probe detected homologous sequences in the dna of only four members of the enterobacteriaceae (citrobacter freundii, salmonella typhimurium, klebsiella pneumoniae, and enterobacter aerogenes); no homology was detected with the dna of ...	1984	6384181
enzymatic profiles of enterobacter sakazakii and related species with special reference to the alpha-glucosidase reaction and reproducibility of the test system.	the enzymatic profiles of enterobacter sakazakii, enterobacter cloacae, enterobacter aerogenes, and enterobacter agglomerans were determined with the api zym system (api system s.a., la balme les grottes, france). each assay was performed three times. a simple formula was derived and applied to assess the reproducibility of the api zym tests. in addition, a separate alpha-glucosidase test was performed. all e. sakazakii isolates produced alpha-glucosidase, in contrast to the other enterobacter i ...	1984	6386874
vaccination against klebsiella aerogenes.	klebsiella vaccine was prepared from strains of klebsiella aerogenes with capsular types k1, k36, k44 and k cross (a type which cross-reacts in vitro with sera from many klebsiella capsular types). the vaccine was extracted by dialysis and ultrafiltration from capsular material released during growth of the bacteria in a five-day batch culture. mice given one dose of vaccine from k1a (1.0 microgram/mouse) survived lethal intraperitoneal challenge of 11/11 homologous klebsiella strains four days ...	1984	6389699
influence of nutrient limitation and growth rate on the outer membrane proteins of klebsiella aerogenes nctc 418.	four major proteins with molecular weights of 78 000, 37 000, 34 000 and 20 000 were present in the envelope of klebsiella aerogenes when cultured at a high specific growth rate. however, at lower growth rates, the protein content and composition of the envelope depended on the imposed nutrient limitation. under potassium-, carbon-, sulphur- and phosphorus-limited conditions, derepression of synthesis of limitation-specific proteins was observed, their apparent molecular weights being 90 000, 48 ...	1984	6389768
vaccination against 77 capsular types of klebsiella aerogenes with polyvalent klebsiella vaccines.	a method is described for producing monovalent and polyvalent vaccines from culture filtrates of klebsiella aerogenes. with a single injection, each monovalent vaccine protected mice against lethal intraperitoneal challenge by more than 30 capsular types; and polyvalent vaccines containing 2-12 monovalent components protected against 46-61 of the 77 capsular types of k. aerogenes. one vaccine with 12 components, administered in two doses, induced full protection against 71 types and protected ha ...	1984	6389878
expression and loss of the pbr322 plasmid in klebsiella aerogenes nctc 418, grown in chemostat culture.	klebsiella aerogenes harbouring the plasmid pbr322 was grown in continuous culture at various growth rates under glucose, phosphate or ammonia limitation. with tetracycline in the medium, the maximum culture beta-lactamase activity was found at the higher growth rates. when tetracycline was absent, loss of resistance to the drug occurred. concomitant with the occurrence of drug-sensitive cells, the culture beta-lactamase activity decreased. at the higher growth rates the enzyme activity decrease ...	1984	6395803
[in vivo interferon-inducing capacity of a multibacterial extract: prospective new use].	we studied ifn inducing ability on syngenic c3h mice of a multibacterial antigenic extract consisting of different strains of following species: staphylococcus aureus, escherichia coli, aerobacter aerogenes, proteus vulgaris. a dose-dependent ifn production was demonstrated and two peaks of circulating ifn were observed few hours and two days after i.v. inoculation. per os administration only at high doses induced evaluable levels of ifn in the circulation.	1984	6399271
extended superfamily of short alcohol-polyol-sugar dehydrogenases: structural similarities between glucose and ribitol dehydrogenases.	the recently determined primary structure of glucose dehydrogenase from bacillus megaterium was scanned by computerized comparisons for similarities with known polyol and alcohol dehydrogenases. the results revealed a highly significant similarity between this glucose dehydrogenase and ribitol dehydrogenase from klebsiella aerogenes. sixty-one positions of the 262 in glucose dehydrogenase are identical between these two proteins (23% identity), fitting into a homology alignment for the complete ...	1984	6420186
distribution and effects of a defined six-member murine-derived microflora in gnotobiotic gerbils.	the gnotobiotic gerbil was selected as a model with which to study the effects of colonization with a defined microflora on organ morphology, histology, and selected blood biochemical parameters. gerbils were maintained germfree for 13 months but failed to reproduce, presumably because of the enlarged cecum. a colony of gnotobiotic gerbils that was associated with a bacterial flora consisting of lactobacillus brevis, streptococcus faecalis, staphylococcus epidermidis, bacteroides vulgatus, enter ...	1984	6426388
the uptake and utilization of entodinium caudatum, bacteria, free amino acids and glucose by the rumen ciliate entodinium bursa.	washed suspensions of entodinium bursa were incubated anaerobically with entodinium caudatum, ten species of bacteria and a yeast. the rate of uptake and digestion of these micro-organisms was investigated. protozoa grown in vivo did not engulf proteus mirabilis or klebsiella aerogenes but rapidly took up bacillus megaterium. selenomonas ruminantium, torulopsis glabrata and streptococcus bovis, although only the last was digested with release of soluble material into the medium. protozoa grown i ...	1984	6427172
an extended model for transfer of micro-organisms via the hands: differences between organisms and the effect of alcohol disinfection.	a model for contact transfer of micro-organisms by hand has been extended to include representatives of bacterial species responsible for a majority of hospital-acquired infections. the ability of the organisms to transfer from contaminated fabrics to hands and from hands to sterile fabrics was measured, as was their ability to survive on the skin of the hands. there were differences between the species. staphylococcus saprophyticus transferred well to the hand but not as well from hand to fabri ...	1984	6429238
stimulation by glutamine of the formation of n6-hydroxylysine in a cell-free extract from aerobacter aerogenes 62-1.	glutamine may serve as an activator and/or regulator of the n6-hydroxylase (e.c. 1.14.99) of aerobacter aerogenes 62-1. activation and stabilization of n6-hydroxylase activity was observed both in vivo and in vitro. growth in a glutamine-supplemented medium resulted in (1) maximum n6-hydroxylase activity at an earlier stage of growth and (2) higher n6-hydroxylase activity and continued aerobactin synthesis into stationary phase. storage of p2 in the presence of l-glutamine (1 mm) significantly i ...	1984	6432805
[transfer of kanamycin resistance among familial strains of enterobacteriaceae isolated from a chronic bacterial carrier of salmonella schottmuelleri].	escherichia coli, enterobacter aerogenes and s. schottmuelleri were isolated from the large intestine of a bacteriocarrier. e. coli and e. aerogenes strains proved to be resistant to a number of antibiotics. plasmids were detected in dna preparations obtained from e. coli strains. after the hybridization of these e. coli strains with e. coli c600 5k and s. schottmuelleri at 28 degrees c the transfer of resistance to kanamycin was found to occur. from some of the transconjugates thus obtained res ...	1984	6438967
influence of metabolic end-products on the growth efficiency of klebsiella aerogenes in anaerobic chemostat culture.	progressively increasing the input concentration of growth-limiting nutrient (glucose, ammonia, k+) to anaerobic chemostat cultures of klebsiella aerogenes (d = 0.38 h-1; 35 degrees c; ph 6.8) led to a non-linear increase in bacterial cell concentration. at modest population densities, residual growth-limiting substrate levels increased substantially, with increasing input concentration, and the culture bacterial dry weight tended to a constant value. with the glucose-limited culture, increasing ...	1984	6442120
genetic and physical maps of klebsiella aerogenes genes for histidine utilization (hut).	deletion derivatives of the hut-containing plasmid pcb101 were tested against point mutants defective in individual genes of the histidine utilization (hut) operons using a complementation/recombination assay. location of the genes of the right operon, hutu and huth, was confirmed by direct assay of the gene products, urocanase and histidase; location of the repressor gene was identified by measuring the ability of the plasmid-carried genes to repress the formation of histidase from a chromosoma ...	1984	6361501
five cephalosporins: pharmacokinetics and their relation to antibacterial potency.	in a group of adult volunteers, pharmacokinetic profiles of five cephalosporins were correlated with their minimal inhibitory concentrations (mics90) against staphylococcus aureus, streptococcus pyogenes, streptococcus pneumoniae, escherichia coli, klebsiella pneumoniae, proteus mirabilis, and enterobacter aerogenes. subjects received the following intravenous regimens in a randomized, crossover fashion: (1) 0.5 gm, 1 gm, or 2 gm of cefazolin; (2) 2 gm of cephalothin; (3) 1 gm of cephapirin; (4) ...	1984	6467282
in vitro and in vivo antibacterial properties of fk 027, a new orally active cephem antibiotic.	fk 027 was more active than cefaclor, cephalexin, and amoxicillin against stock strains of a wide variety of gram-negative bacteria, including such opportunistic pathogens as citrobacter and enterobacter species and serratia marcescens. fk 027 was significantly more active than the three reference drugs against clinical isolates of escherichia coli, klebsiella pneumoniae, indole-positive and -negative proteus species, providencia species, haemophilus influenzae, and neisseria gonorrhoeae. it was ...	1984	6561017
bile acid deconjugation and attachment of chicken gut bacteria: their possible role in growth depression.	bacteria isolated from the chicken gut were tested for their ability to deconjugate bile acids and attach to chicken epithelial cells (crop squamous cells and duodenal brush borders). clostridium perfringens, streptococci and some of the bifidobacteria and lactobacilli were able to deconjugate all 4 substrates whereas the bacteroides deconjugated only the taurine conjugates and the coliforms were completely inactive. none of the strains of escherichia coli or streptococci attached to squamous ce ...	1984	6733554
mezlocillin and ticarcillin alone and combined with gentamicin in the treatment of experimental enterobacter aerogenes endocarditis.	the efficacies of mezlocillin and ticarcillin, each alone and in combination with gentamicin, in the therapy of experimental left-sided enterobacter aerogenes endocarditis in rabbits were compared. each beta-lactam was administered intramuscularly at a dose of 180 mg/kg every 6 h either alone or with gentamicin (1.7 mg/kg intramuscularly every 8 h). bacterial populations at the start of therapy (7 days after initiation of infection) were 9 to 10 log10 cfu/g of vegetation. ticarcillin produced co ...	1984	6742813
regulation of hutuh operon expression by the catabolite gene activator protein-cyclic amp complex in klebsiella aerogenes.	rna polymerase transcribed the hutuh operon of klebsiella aerogenes if the catabolite gene activator protein (cap) and cyclic amp (camp) were present or if the dna template was derived from a promoter mutant in which hutuh expression was independent of the need for positive effectors. in the absence of cap or camp, not only was hutuh transcription absent, but transcription in the opposite direction (toward hutc) was initiated at a site (pc) ca. 70 base pairs from the site (puh) of hutuh mrna ini ...	1984	6090399
[clinical laboratory approach for estimating effective administrative dosage of cefmenoxime. observation on the mics and cefmenoxime disc susceptibility test].	the in vitro activity of cefmenoxime (cmx) was determined using agar dilution at inoculum level of 10(6) cfu/ml against 333 clinical bacterial isolates. cmx was highly active against escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter aerogenes and haemophilus influenzae and also streptococcus pyogenes and streptococcus pneumoniae with mic values in the range of 0.024 to 3.13 micrograms/ml. against staphylococci and serratia marcescens, cmx showed the antimicrobial activity ...	1984	6090721
[clinical problems of surgical infection. the pirogov institute for emergency medicine, sofia].	the aetiology, pathogenesis, diagnosis and therapy of sepsis are dealt with in this paper. these problems are discussed on the basis of 151 patients treated for sepsis. the cases with monoinfection are 73.6% and those with polyinfection are 24.4%. monoinfection is caused mainly by staphylococci -65,3%, followed by e. coli - 15.2%, proteus - 13% and klebsiella - 3.3%. for the cases of polyinfection the gram-negative bacteria are 3:1 in respect to the gram-positive bacteria. the bacteriological fi ...	1984	6150608
antibacterial activity and kill kinetics of ampicillin/sulbactam (cp-45899) combinations against escherichia coli and klebsiella aerogenes.	combinations of ampicillin and sulbactam (cp-45899) were tested against 11 strains of escherichia coli and five strains of klebsiella aerogenes. with the exception of one e. coli, strain, the strains were all highly resistant to ampicillin. synergy was demonstrated by agar dilution in six of the ten resistant e. coli strains and in three of the five k. aerogenes strains. at simulated in vivo conditions with constantly decreasing concentrations mimicking in vivo pharmacokinetics, synergy was dete ...	1984	6323321
plasmid- and chromosome-coded aerobactin synthesis in enteric bacteria: insertion sequences flank operon in plasmid-mediated systems.	large plasmids were detected in two aerobactin-producing enteric bacterial species (aerobacter aerogenes 62-i, salmonella arizona sa1, and s. arizona sl5301) and designated psmn1, psmn2, and psmn3, respectively. other salmonella spp., namely, s. arizona sl5302, s. arizona sls, salmonella austin, and salmonella memphis, formed aerobactin but contained no detectable large plasmids. s. arizona sl5283 made no aerobactin. a probe consisting of the aerobactin biosynthetic genes cloned on plasmid pabn5 ...	1984	6330037
transposon tn5 encodes streptomycin resistance in nonenteric bacteria.	strains of caulobacter crescentus, pseudomonas putida, acinetobacter calcoaceticus, rhizobium meliloti, and rhodopseudomonas sphaeroides carrying the kanamycin resistance-encoding transposon tn5 were 15 to 500 times more resistant to streptomycin than transposon-free strains. the streptomycin resistance determinant, which is separable from the kanamycin resistance determinant of tn5, was not expressed in escherichia coli or klebsiella aerogenes.	1984	6330041
comparative in vitro activity and beta-lactamase stability of fr 17027, a new orally active cephalosporin.	fr 17027, a new orally absorbed cephalosporin ester, inhibited group a and b streptococci and streptococcus pneumoniae at less than or equal to 0.1 micrograms/ml, which is similar to the inhibition concentration of amoxicillin and cefaclor, and was more active than cephalexin. it was less active (mic, 25 micrograms/ml) against staphylococci than was cephalexin, and it did not inhibit streptococcus faecalis or listeria monocytogenes. fr 17027 inhibited beta-lactamase-producing isolates of neisser ...	1984	6333207
[substrate specificity of the beta-lactamases found in a number of clinical strains of gram-negative bacteria].	the substrate profiles of beta-lactamases defected in 46 clinical polyresistant strains of gram-negative bacteria were determined. by the substrate profile and sensitivity to inhibitors (dicloxacillin and p-cmb) beta-lactamases were considered to belong to classes i, ii, iii, iv and v of the richmond classification. the molecular weights of the enzymes were measured. enterobacter aerogenes 6803, enterobacter aerogenes 11030 and klebsiella pneumoniae 970 produced simultaneously two beta-lactamase ...	1984	6334489
synergism of ps-5 with penicillins and cephalosporins in antimicrobial activity against beta-lactam-resistant gram-negative microorganisms.	the in vitro synergism of ps-5 combined with various penicillins and cephalosporins in antimicrobial activity was examined in detail against beta-lactam-resistant gram-negative bacteria. ps-5 showed a highly significant synergism in antimicrobial action against escherichia coli rgn238 in combination with penicillins; and against proteus vulgaris gn76 and serratia marcescens t55 in combination with cephalosporins. it was moderately synergistic against citrobacter freundii gn346, enterobacter cloa ...	1984	6334679
[isolation and properties of beta-lactamase of the cephalosporinase type from cells of enterobacter aerogenes 6803].	beta-lastamase with the molecular weight of 32500 was isolated from the cells of clinical strain 6803 of enterobacter aerogenes and purified. by the substrate profile determined microiodometrically beta-lactamase was classified as belonging to the cephalosporinase type. the activity of the electrophoretically homogenous enzyme was equal to 430 microm a minute per mg protein with respect to benzylpenicillin. the km for benzylpenicillin, dicloxacillin, cephaloridin and cephalothin was 6.5410(-5), ...	1984	6335380
recombination-induced suppression of cell division following p1-mediated generalized transduction in klebsiella aerogenes.	klebsiella aerogenes recombinants resulting from bacteriophage p1-mediated generalized transduction failed to increase in number for approximately six generations after transduction. nevertheless these recombinants continued to grow and became sensitive to penicillin after a transient resistance, suggesting that the cells were growing as long, non-dividing filaments. when filamentous cells were isolated from transduced cultures by gradient centrifugation, recombinants were 1000-fold more frequen ...	1983	6343791
gene transfer between escherichia coli and enterobacter aerogenes.		1983	6343802
discoidin-binding polysaccharide from dictyostelium discoideum.	extracts of dictyostelium discoideum grown axenically in a chemically defined medium were evaluated for binding to discoidin i and discoidin ii, endogenous lectins of this slime mold. binding activity was measured by competitive inhibition of 125i-lactosyl-bovine serum albumin binding to the immobilized lectins. with the solubilization procedure used extracts of vegetative cells and of early aggregates had no significant inhibitory activity, but an abundant discoidin-binding substance was detect ...	1983	6345545
relative substrate affinity index values: a method for identification of beta-lactamase enzymes and prediction of successful beta-lactam therapy.	using a nitrocefin competition assay, i determined the relative substrate affinity index (rsai) values of nine clinically significant beta-lactamase enzymes against a range of beta-lactams. using selected beta-lactam substrates, i observed large differences in the rsai values of the nine enzymes that were sufficient in many cases to positively identify specific enzymes. i made use of the unique rsai values of shv-1, tem-1, and tem-2 beta lactamases with cefoxitin to screen for the presence of th ...	1983	6345576
metabolic and energetic aspects of the growth of klebsiella aerogenes nctc 418 on glucose in anaerobic chemostat culture.		1983	6347115
s-acylated residues of the acyl-carrier protein subunit of klebsiella aerogenes citrate lyase.	oxidation of the isolated deacetyl acyl-carrier protein subunit of citrate lyase from klebsiella aerogenes with cu2+-o-phenanthroline complex leads exclusively to intrapeptide disulfide bridge formation indicating that the cysteamine and the cysteine residues are located in close proximity. the s-acetylation of the cysteine residue in deacetyl acyl-carrier protein subunit is catalysed by a citrate lyase ligase preparation in presence of acetate and atp. reaction-inactivation of citrate lyase res ...	1983	6349627
evaluation of ceftazidime in the treatment of severe bacterial infection.	we investigated the clinical efficiency and safety of ceftazidime for treatment of 33 episodes of infection in 30 patients (17 males and 13 females), whose ages ranged from 9 to 92 years (mean 52.5). fourteen patients had ultimately fatal disease and the remaining 16 had non-fatal diseases. the clinical condition of patients at the beginning of treatment was critical or poor in 16 cases. episodes of infection treated were: 24 urinary tract infections (eight of them with concomitant bacteraemia), ...	1983	6352616
nitrogen regulation system of klebsiella aerogenes: the nac gene.	in klebsiella aerogenes, the product of a his-linked gene, nac, appears to play a crucial role in tying the synthesis of enzymes activated or repressed by ammonia deprivation, such as histidase and glutamate dehydrogenase, to the known regulators of nitrogen assimilation, the products of glng and glnf.	1983	6352688
regulation of metabolite overproduction in klebsiella aerogenes.		1983	6353539
[influence of hemolytic plasmids on the development of the "killing" effect in various species of enterobacteria].	experiments on isogenic strains of different enterobacterial species, both carrying hemolytic plasmids and free from them, have demonstrated that their "killing" effect on mice is linked with the yield of hemolysin and does not depend on the origin of hly plasmids or the chemotype of bacteria. plasmid p ie 567 coding the synthesis of alpha-hemolysin has been shown to be capable of expression in enterobacter aerogenes, hafnia alvei and salmonella typhimurium strains, but e. aerogenes 3/43, h. alv ...	1983	6356727
identification of major sulfate conjugates in the metabolism of propranolol in dog and man.	a large portion of the dose of propranolol in animals and man is unaccounted for. using radiotracer and hplc techniques, five previously unrecognized polar and labile metabolites were found in dog urine, together accounting for 34% of the urinary radioactivity. the two main metabolites, peak 2 (7% of the radioactivity) and peak 4 (17%) could be isolated and purified by butanol extraction and reversed phase hplc. direct probe ms analysis of the main peak 4 and gc/ms analysis of the same peak afte ...	1983	6137341
the role of the o and k antigens in determining the resistance of klebsiella aerogenes to serum killing and phagocytosis.	the presence of both k and o antigens of klebsiella aerogenes was found necessary to protect the organism from either complement-mediated serum killing or phagocytosis in the absence of specific antisera. optimal phagocytic ingestion of k. aerogenes nctc 5055 could be achieved in the presence of either anti-k or anti-o sera or to a much smaller extent in antisera raised against a rough unencapsulated mutant (m10b) derived from nctc 5055. anti-o sera failed to opsonize a clinical klebsiella isola ...	1983	6195306
survival of multiply-resistant klebsiella aerogenes and other gram-negative bacilli on finger-tips.	the survival of various gram-negative bacilli was evaluated by inoculating the finger-tips of volunteers and determining the number of recoverable organisms in finger washings taken at increasing time intervals up to 60 min. three epidemic gentamicin-resistant multiply-resistant strains of klebsiella aerogenes (capsular types k2, k16 and k21) survived better than pseudomonas aeruginosa, escherichia coli or non-epidemic antibiotic sensitive klebsiellae of corresponding capsular type. the survival ...	1983	6198364