Publications

[susceptibility of vibrio cholerae el-tor strains to various antibiotics].	in this study, the in-vitro activity of ampicillin, chloramphenicol, oxytetracycline, trimethoprim-sulfamethoxazole (tmp-smx), cefoperazone, ceftriaxone, cefotaxime, ceftizoxime, ofloxacin, pefloxacin, ciprofloxacin, and fleroxacin against clinically isolated strains of v. cholerae biotype el-tor have been investigated. the minimum inhibitory concentrations (mics) were determined using the microtube dilution technique except for tmp-smx which was tested by agar dilution technique. all of el-tor ...	1988	3273600
radioimmunoscintigraphy of xenografted human thyroid carcinoma.	we developed monoclonal antibodies against human thyroid cancer-associated antigen by fusing mouse myeloma cells with mouse spleen cells immunized by insoluble fraction of homogenized thyroid papillary carcinoma cells. one monoclonal antibody (ktc-3, igm) was selected to evaluate basic usefulness for radioimmunoscintigraphy in xenografted human thyroid carcinoma. ktc-3 was labeled with 131i by iodogen method of 20 to 1 iodogen to igm molar ratio. it was also labeled with 111in by cyclic dtpa anh ...	1988	3275101
[evaluation of two experimental models of end-to-side microvessel anastomosis in rats].		1988	3151320
[use of gnotobiotic animals for the study of the pathogenesis of cholera intoxication].	germ-free suckling rabbits and minipigs can be recommended as models suitable for the study of different aspects of the pathogenesis of cholera intoxication. in minipigs, individual representatives of intestinal autochthonous microflora produce different effect on the sensitivity of the animals to the toxigenic and choleragenic action of vibrio cholerae antigen introduced by oral administration, that should also be taken into consideration in the determination of residual toxicity during the tri ...	1988	2469271
transient outward current prominent in canine ventricular epicardium but not endocardium.	previous studies have denied the presence of a transient outward current (ito) in ventricular myocardium of dog, sheep, and calf. using conventional microelectrode techniques, we provide evidence for a significant contribution of ito to epicardial, but not endocardial, activity of canine ventricular myocardium. the epicardial action potential when compared with that of endocardium shows a smaller phase 0 amplitude, a much more prominent phase 1, and a phase 2 amplitude that is greater than that ...	1988	2826039
transient outward current prominent in canine ventricular epicardium but not endocardium.	previous studies have denied the presence of a transient outward current (ito) in ventricular myocardium of dog, sheep, and calf. using conventional microelectrode techniques, we provide evidence for a significant contribution of ito to epicardial, but not endocardial, activity of canine ventricular myocardium. the epicardial action potential when compared with that of endocardium shows a smaller phase 0 amplitude, a much more prominent phase 1, and a phase 2 amplitude that is greater than that ...	1988	2826039
virus enhances histamine release from human basophils.	histamine release from human basophil leukocytes was triggered by staph. aureus or by complement activation caused by endotoxins isolated from e. coli or salmonella bacteria. influenza a virus was found to enhance the mediator release and the effect was caused by synergism, since the virus itself did not release histamine. the potentiating effect of the virus was abolished by a potent neuraminidase inhibitor. furthermore, a purified neuraminidase preparation obtained from vibrio cholerae caused ...	1988	2455978
transfer of antibiotic resistances from bacillus spp. to staphylococcus aureus and vibrio cholerae.		1988	2456988
adjuvant effect of deae-dextran on cholera vaccines.	investigations into the effectiveness of deae-dextran as an adjuvant of whole cell cholera vaccines (vibrio cholerae inaba and ogawa serotypes) were conducted using two methods: (a) the active mouse protection test, (b) the determination of antibody production in the sera of immunized mice by measuring the level of vibriocidal antibodies, and by an enzyme-linked immunosorbent assay (elisa). in all tests, the deae-dextran-adjuvanted vaccine showed an antigenicity substantially superior to that ac ...	1988	2458658
distinct domains of recombinant human ifn-gamma responsible for anti-viral effector function.	a panel of 21 independently isolated neutralizing mab directed against the human rifn-gamma (rhuifn-gamma) was used to characterize those epitopes that are involved in the antiviral function of the rhuifn-gamma. a sandwich competition assay was developed to determine the cross-reactivities between the mab. the 125i-labeled mab were allowed to compete with varying amounts of unlabeled mab for binding to rhuifn-gamma under ag-limiting conditions, and the 50% inhibition endpoints were determined fo ...	1988	2459212
frequency analysis of simian virus 40-specific cytotoxic t lymphocyte precursors in the high responder c57bl/6 mouse strain.	studies in this laboratory have shown that long term simian virus 40 (sv40)-specific cytolytic t lymphocyte (ctl) cultures established from the spleens of high responder c57bl/6 (b6; h-2b) mice exhibit a preference for the selection of h-2db-restricted ctl clones. in this study, we have investigated the basis for this selection. limiting dilution cultures were established using responder cells from the popliteal lymph nodes and the spleens of b6 mice immunized subcutaneously in the hind footpads ...	1988	2459302
[auxotrophic mutants of cholera and nonagglutinating vibrios and a method of their determination].		1988	2459483
[a minimal synthetic medium for determining cystine desulfohydrolase in vibrio cholerae].		1988	2461482
[the population composition of cultures of vibrio cholerae strain 569 b pakistan in relation to the manufacture of a cholera vaccine (choleragen-anatoxin+o-antigen)].	the study of different subcultures of v. cholerae strain 569 b pakistan, used as the source of choleragen in the production of cholera vaccine, has been made. the composition of the population formed by this strain has been found to be heterogeneous with respect to toxin formation. the control of changes occurring in the production culture by analyzing its population composition has been proposed.	1988	2461623
[environmental phages active in relation to vibrios of the non-01 group].		1988	2462125
demonstration of lipopolysaccharide on sheathed flagella of vibrio cholerae o:1 by protein a-gold immunoelectron microscopy.	monoclonal antibodies with group and type specificity for lipopolysaccharide antigens were used in combination with protein a-colloidal gold labeling and transmission electron microscopy to demonstrate the presence of lipopolysaccharide antigens on both the sheathed flagellum and the cell surface of inaba and ogawa strains of vibrio cholerae o:1. labeling was associated with the sheath of the flagellum rather than the core, and flagellar cores were not labeled. flagellum and cell shared a common ...	1988	2450866
influenza a virus potentiates basophil histamine release caused by endotoxin-induced complement activation. examination of normal individuals and patients with intrinsic asthma.	histamine release from human basophil leukocytes was triggered by complement activation by means of endotoxins isolated from e. coli and salmonella bacteria. influenza a virus was found to enhance the mediator release, and the effect was caused by synergism, since virus itself did not release histamine. the potentiating effect was similar in cells from normal individuals and from patients with intrinsic asthma. the involvement of viral neuraminidase was examined by a potent neuraminidase inhibit ...	1988	2452582
recombinant gibbon interleukin-3 acts synergistically with recombinant human g-csf and gm-csf in vitro.	recombinant gibbon interleukin-3 (il-3) is a multilineage hematopoietic colony-stimulating factor (csf) that recently was cloned and found to be highly homologous with human il-3. gibbon il-3, as well as human granulocyte-csf (g-csf) and human granulocyte-macrophage csf (gm-csf), stimulated normal human bone marrow cells to form myeloid colonies in soft agar in a sigmoidal dose-response manner. when il-3 was added to increasing concentrations of g-csf or gm-csf, synergistic colony formation occu ...	1988	2453230
nucleotide sequences and comparison of the hemolysin determinants of vibrio cholerae el tor rv79(hly+) and rv79(hly-) and classical 569b(hly-).	we determined the nucleotide sequences of the hemolysin structural gene, hlya, of vibrio cholerae el tor biotype strains rv79(hly+) and rv79(hly-) and the hly determinant of the nonhemolytic classical biotype strain 569b(hly-). the sequences of the hlya gene from el tor strains rv79(hly+) and rv79(hly-) have an identical 2,223-base open reading frame which is predicted to encode an 81,977-dalton precursor form of hemolysin. this value is in excellent agreement with the 84,000-mr hemolysin descri ...	1988	2453464
[characteristics of the formation of hydrogen sulfide by cholera vibrios].		1988	2453707
studies on the vibrio cholerae mucinase complex. iii. neutralisation of the neuraminidase activity by specific anti-neuraminidase igg.	a partially-purified neuraminidase from the mucinase complex of vibrio cholerae was used to prepare a specific anti-neuraminidase antiserum in rabbits. when the neutralising potency of this serum against v. cholerae neuraminidase was assessed in conventional tests, the enzymic activity, as measured by thiobarbituric acid, methoxyphenol-neuraminate and goblet-cell assays, apparently increased. these results are attributable to the presence of a sialylated glycoprotein substrate and small amounts ...	1988	2448460
the development of a bivalent vaccine against diarrhoeal disease.	the live oral typhoid vaccine salmonella typhi ty2la has been successfully used as an effective public health tool for the control of typhoid fever. this paper reviews the progress of one vaccine development programme, which uses this organism as a carrier of the o-antigens of vibrio cholerae. it is already known that antibodies directed against the o-antigens have been previously demonstrated in animals to be protective against subsequent challenge with virulent organisms. this paper reports th ...	1988	2464195
infection and immunity to vibrio cholerae, salmonella typhimurium and escherichia coli in a rabbit model.	cholera disease can be induced in the rabbit by duodenal inoculation (di) of vibrio cholerae organisms after ligation of the cecum (c) (dic model). when ligation of the cecum is omitted, no disease symptoms develop. in contrast, the animals are primed which becomes apparent as vibriocidal protection upon challenge with v. cholerae in the dic model. this protection coincides with high anti-o antigen iga levels in the bile. the o antigen was shown to be the protective antigen and it must be presen ...	1988	2464884
comparison of hemolysins of vibrio cholerae non-o1 and vibrio hollisae with thermostable direct hemolysin of vibrio parahaemolyticus.	hemolysin (vh-rtdh) produced by vibrio hollisae and hemolysin (nag-rtdh) produced by vibrio cholerae non-o1 were characterized and compared with hemolysin (vp-tdh) produced by vibrio parahaemolyticus. these three hemolysins are each composed of two subunits and have similar, but not identical, molecular weights. the amino acid compositions of vp-tdh and nag-rtdh are similar, but are different from that of vh-rtdh. the three hemolysins showed similar lethal toxicities to mice. the effects of temp ...	1988	2466545
ca dependence of na influx during treatment of rabbit aorta with ne and high k solutions.	cellular influx of 24na was measured in isolated rabbit aorta during stimulation with 10 microm norepinephrine (ne) or depolarization with 80 mm k solution, using a pulse-labeling, cold-wash technique. ne caused a two- to threefold increase in na influx; a smaller but significant increase was also observed in depolarized tissues. basal and ne-induced fluxes at 1 min were significantly increased by a 20-min preincubation in a ca-free solution containing 2 mm egta; elevation of [mg] in this soluti ...	1988	2447797
determinants of immunogenicity and mechanisms of protection by virulent and mutant vibrio cholerae o1 in rabbits.	the colonizing capacities of 16 vibrio cholerae strains, including nine genetically and/or phenotypically defined parent-mutant pairs, were determined in unobstructed adult rabbit small bowel. there were marked interstrain differences in colonizing capacity, which was enhanced by bacterial motility and the production of cholera holotoxin but was unrelated to production of cholera toxin b-subunit or hemolysin or to bacterial serotype or biotype. the role of colonizing capacity and other bacterial ...	1988	2447017
effect of endogenous and exogenous interferons on the differentiation of human monocyte cell line u937.	the effect of interferons (ifns) on the differentiation of hematopoietic cells was examined with the human monocyte cell line u937. the differentiation of u937 was induced by hydroxyvitamin d3 and was evaluated through the study of specific markers. the induction of the u937 differentiation was associated with a production of ifn and with a marked increase in (2'5') oligoadenylate synthetase. addition of anti-ifn-alpha/beta antibodies inhibited the enhancement of (2'5') oligoadenylate synthetase ...	1988	2446743
immunosuppressive effect of fk506 on collagen-induced arthritis in rats.	fk506, a new immunosuppressive agent, was given intramuscularly to rats for 12 days, starting on the day of type ii collagen immunization. fk506 in doses of 0.32 mg/kg or more suppressed arthritis and also suppressed humoral and skin test response to type ii collagen. fk506 suppressed arthritis only when given during the afferent limbs of immune response (0-4 days), whereas the drug was only marginally effective when treatment was started during the efferent limbs of immune response (7-11 days). ...	1988	2446810
a novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in vibrio cholerae requires toxr.	the toxr gene of vibrio cholerae encodes a transmembrane, dna-binding protein that activates transcription of the cholera toxin operon and a gene (tcpa) for the major subunit of a pilus colonization factor. we constructed site-directed insertion mutations in the toxr gene by a novel method employing the chromosomal integration of a mobilizable suicide plasmid containing a portion of the toxr coding sequence. mutants containing these new toxr alleles had an altered outer membrane protein profile, ...	1988	2836362
adp-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and naf.	we have developed a method to adp-ribosylate the stimulatory guanine nucleotide-binding protein of adenylate cyclase (gs) in brain membranes by using cholera toxin. in particular, we used isonicotinic acid hydrazide and 3-acetylpyridine adenine dinucleotide to inhibit the potent nad-glycohydrolase activity of brain membranes, and we used the detergent triton x-100 (at 0.1%) to improve the accessibility of the toxin and guanine nucleotides used for supporting the adp-ribosylation. this method rev ...	1988	2836559
adp-ribosylation by cholera toxin of membranes derived from brain modifies the interaction of adenylate cyclase with guanine nucleotides and naf.	we have developed a method to adp-ribosylate the stimulatory guanine nucleotide-binding protein of adenylate cyclase (gs) in brain membranes by using cholera toxin. in particular, we used isonicotinic acid hydrazide and 3-acetylpyridine adenine dinucleotide to inhibit the potent nad-glycohydrolase activity of brain membranes, and we used the detergent triton x-100 (at 0.1%) to improve the accessibility of the toxin and guanine nucleotides used for supporting the adp-ribosylation. this method rev ...	1988	2836559
treatment of cytomegalovirus infections in renal transplant recipients with 9-(1,3-dihydroxy-2-propoxymethyl) guanine.		1988	2837848
immune response of chickens inoculated with a recombinant avian leukosis virus.	specific-pathogen-free embryos (18-day incubation) and hatched chicks were inoculated with a recombinant avian leukosis virus (alv) produced by recombinant dna techniques. enzyme-linked immunosorbent assays were used to measure the production of viral-protein-specific antibody and the viral protein, p27, in the serum at 2, 5, 8, 14, and 20 weeks of age. of the inoculated chickens surviving to 20 weeks, 64% produced viral-protein-specific antibodies and 42% transiently produced the viral protein, ...	1988	2838018
mechanism of the epsp synthase catalyzed reaction: evidence for the lack of a covalent carboxyvinyl intermediate in catalysis.	in order to detect covalent reaction intermediates in the 5-enolpyruvylshikimate-3-phosphate (epsp) synthase reaction, we have investigated the interaction of epsp synthase with the reaction product epsp. an exchange of epsp-methylene protons could be demonstrated by incubating epsps with epsp in d2o. since trace amounts of contaminating pi would lead to reversal of epsps reaction and hence methylene proton exchange, we added pyruvate kinase, adp, mg++ and k+. under these conditions, any contami ...	1988	2838023
absence of an endogenous regulator of na+,k+-atpase activity in the tissues of cirrhotic rats.	microsomal na+,k+-atpase isolated from the renal cortex of rats with ccl4-induced cirrhosis (cir) showed a higher specific activity than the enzyme obtained from control rats (cor). kinetic studies showed a lower k0.5 for atp (0.08 +/- 0.03 vs. 0.24 +/- 0.04 mm; p less than 0.05), a lower na+ activation constant (9.6 +/- 1.5 vs. 19.0 +/- 1.7 mm; p less than 0.05), and a higher k+ activation constant (1.2 +/- 0.1 vs. 0.6 +/- 0.1 mm; p less than 0.05) for cir. the optimal ph of the enzyme was 0.5 ...	1988	2838057
human and simian immunodeficiency retroviruses: activation and differential transactivation of gene expression.	new west african human immunodeficiency viruses (hiv-2s) and simian immunodeficiency virus (siv) contain functional transactivator (tat) gene and tat response elements. their long terminal repeats (ltr) and tat genes are more related among themselves than to hiv-1 ltr and tat gene. the viral gene expression of hiv-2 as well as siv can be stimulated by t cell activators, such as mitogens and phorbol esters. hiv-2 and siv display a much broader transactivation response specificity than does hiv-1. ...	1988	2840105
selective inhibition of arachidonate 5-lipoxygenase by novel acetohydroxamic acids: biochemical assessment in vitro and ex vivo.	1. the chemically novel acetohydroxamic acids, bw a4c, bw a137c and bw a797c, are potent inhibitors of the synthesis of leukotriene b4 (ltb4) from arachidonic acid by human leucocyte homogenates: the concentrations required for 50% inhibition (ic50) were 0.1 microm, 0.8 microm and 0.5 microm respectively. inhibition was less at higher concentrations of arachidonic acid. 2. these compounds also inhibited the synthesis of [14c]-5-hete from [14c]-arachidonic acid and the calcium-dependent synthesis ...	1988	2840160
developmental changes in myosin isoforms from slow and fast latissimus dorsi muscles in the chicken.	in the course of muscle differentiation, changes in fibre-type population and in myosin composition occur. in this work, the expression of native myosin isoforms in developing fast-twitch (posterior latissimus dorsi; pld) and slow-tonic (anterior latissimus dorsi; ald) muscles of the chick was examined using electrophoresis under nondissociating conditions. the major isomyosin of 11-day-old embryonic pld comigrated with the adult fast myosin fm3. two additional components indistinguishable from ...	1988	2840316
a new hamster model for adenoviral vaccination.	both adult and baby hamsters infected intranasally with human adenovirus type 5 exhibited virologic, serologic, and histologic evidence of infection. when 8-day old hamsters were infected with 4 x 10(6) pfu, concentrations of virus up to 2 x 10(6) pfu/animal were detected in the lung, peaking on day 2. the minimum infectious dose was 1 x 10(3) pfu/animal. this model may be useful in studies of conventional and recombinant adenoviral vaccines for humans.	1988	2840876
effect of parathyroid hormone and forskolin on cytoskeletal protein synthesis in cultured mouse osteoblastic cells.	parathyroid hormone (pth) has been shown to cause transient cell shape changes in bone cells. we have examined the effects of parathyroid hormone and forskolin on the organization and expression of cytoskeletal proteins in cultured mouse endosteal osteoblastic cells. analysis of [35s]methionine-labeled cytoskeletal proteins isolated on two-dimensional gel electrophoresis showed that pth treatment (24 h) stimulated the de novo biosynthesis of actin, vimentin and tubulins in confluent cells, where ...	1988	2840972
molecular defects in cytochrome oxidase in mitochondrial diseases.	defects of cytochrome c oxidase (cox) show remarkable clinical, biochemical, and genetic heterogeneity. clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. immunologically reactive enzyme protein is decreased in some forms of cox deficiency but not in others. because cox is encod ...	1988	2841309
molecular defects in cytochrome oxidase in mitochondrial diseases.	defects of cytochrome c oxidase (cox) show remarkable clinical, biochemical, and genetic heterogeneity. clinically, there are two main groups of disorders, one dominated by muscle involvement, the other by brain dysfunction. biochemically, the enzyme defect may be confined to one or a few tissues (reflecting the existence of tissue-specific isozymes) or affect all tissues. immunologically reactive enzyme protein is decreased in some forms of cox deficiency but not in others. because cox is encod ...	1988	2841309
egg shell quality and cholecalciferol metabolism in aged laying hens.	calcium-binding protein d28k (calbindin) synthesis, vitamin d metabolism and shell quality were investigated in young and aged laying hens fed diets containing either cholecalciferol (cc) or its 1-hydroxylated derivatives. duodenal calbindin concentration was similar in the young and in the aged laying hens. exogenous 1-hydroxylated cc derivatives increased duodenal calbindin concentration, regardless of age. shell weight and shell density (mg/cm2) were significantly lower (p less than 0.01) in ...	1988	2841436
effect of ethanol on neuromuscular function in the rat diaphragm preparation.	the effect of ethanol on neuromuscular transmission in the isolated phrenic nerve-diaphragm preparation of the rat was studied by analysing its effect on directly- and indirectly-elicited twitch tension, tetanic and post-tetanic twitch tensions, and on the phenomenon of post-tetanic twitch potentiation (ptp). the results show that ethanol (1.0 x 10(-4)-1.0 m) depresses neuromuscular transmission, by reducing the amplitudes of both directly and indirectly elicited twitch and tetanic contractions. ...	1988	2844197
[relation between the characteristic activity of the beta-adrenergic agonist isoproterenol and the percolation properties of cell membrane].	variations in the intrinsic activity of 1-isoproterenol and in the percentage of high affinity beta-receptor complexes have been studied under a changes in the macrostructure of reticulocyte plasma membranes. the fluid lipid fraction have been reduced in the membranes for this by phospholipase a2/bsa treatment. it was accompanied by a progressive decrease in the fraction of beta-receptors that are able to form the high affinity complexes with 1-isoproterenol (receptor--regulatory n-protein compl ...	1988	2844324
detection of leukotriene b4 in cardiac tissue and its role in infarct extension through leucocyte migration.	the main left coronary artery of rats was ligated near its origin under light ether anaesthesia and the infarction observed for 48 h. the ischaemic area was determined after an intravenous injection of pontamine sky blue dye 1 h before induction of cardiac arrest with potassium chloride. the unstained area (true ischaemic area) decreased with time to 27.1% of the left ventricle at 48 h, whereas the intensely stained area between the normal and the true ischaemic areas increased with time, sugges ...	1988	2844405
t4 cell activation by immobilized phytohemagglutinin: differential capacity to induce il-2 responsiveness and il-2 production.	soluble mitogens, such as pha induce accessory cell (ac)-dependent t cell proliferation. one function of the ac is to create a stimulatory matrix. therefore, experiments were carried out to determine whether pha immobilized onto microtiter plates could stimulate t cells in the absence of ac. peripheral blood t4 cells were cultured under limiting dilution conditions with either soluble or immobilized pha with or without ril-1 beta, ril-2, r-tnf-alpha, an anti-cd28 mab (9.3), or irradiated ebv-tra ...	1988	2844904
[the diagnosis of infectious mononucleosis in childhood].		1988	2845334
heterogeneity of glycine receptors and their messenger rnas in rat brain and spinal cord.	messenger rnas isolated from adult or newborn rat spinal cord were fractionated in a sucrose gradient. the fractions were injected into xenopus oocytes to determine their potencies for expression of glycine receptors (glyrs), which were then examined electrophysiologically. the sedimentation profiles disclosed two classes of glyr mrnas, one heavy and the other light. the adult spinal cord was rich in heavy glyr mrna, whereas the light glyr mrna was more abundant in neonatal spinal cord and in ad ...	1988	2845580
endobronchial hamartoma associated with bronchioloalveolar cell carcinoma.	computed tomographic findings in a 60-year-old man with lobar bronchioloalveolar cell carcinoma distal to endobronchial hamartoma are described.	1988	2846241
infection control measures for human immunodeficiency virus (hiv): universal precautions.		1988	2846803
txb2: cardiostimulant effect that involves beta-adrenoceptor and na+ + k+-atpase activity.	the biological properties of thromboxane b2 (txb2) on isolated rat heart were studied. its actions were compared with u-46619 a thromboxane a2 mimetic compound and with isoproterenol. txb2 induced a concentration-dependent increase in contractility, that was non-competitively antagonized by propranolol. in addition txb2 inhibited na+ + k+-atpase activity at the same concentrations that influenced the mechanical activity. inhibition of beta-adrenoceptors efficiently blocked the inhibitory action ...	1988	2847200
[effect of bilirubin and serum albumin on the na,k-atpase activity in the synaptosomal membrane].	the absorption spectrum of visible light, characteristic of the free bilirubin being in the aqueous medium, with a single maximum at 440 nm and with the shoulder in the region of 410-420 nm is transformed into the spectrum with two maxima in the region of 460 and 500 nm, respectively, when the pigment is bound in vitro by the synaptosomal membrane. there are two types of sites for bilirubin binding in the membrane particles, differing in the values of constants of association (ka = 0.6 . 10(5) a ...	1988	2847380
influence of immunomodulatory agents on bovine humoral and cellular immune responses to parenteral inoculation with bovine rotavirus vaccines.	sodium diethyldithiocarbamate (dtc), mycobacterium cell wall extract (mcwe, regressin), killed corynebacterium parvum (c. parvum, immunoregulin) and muramyldipeptide (mdp) were each combined with purified, live bovine rotavirus and inoculated into 3 month-old holstein-friesian calves in order to examine their ability to potentiate specific humoral and cellular immune responses. the vaccinated calves were boosted twice at 3 and 6 weeks after initial vaccine inoculation. the rotavirus was administ ...	1988	2847397
blunted cgmp response to anf in vascular smooth muscle cells of shr.	abnormalities in the coupling of atrial natriuretic factor (anf) receptors with the guanosine 5'-cyclic monophosphate (cgmp) system in vascular smooth muscle cells (vsmcs) may play a role in the pathophysiology of hypertension in the spontaneously hypertensive rat (shr). this concept was examined in cultured, aortic vsmcs (passages 6-10) from shr, wistar-kyoto (wky), and american wistar (wis) rats. quiescent vsmcs of the shr (serum deprived for 24 h) had higher anf receptor density (bmax) and lo ...	1988	2847533
inhibition of porphyrin biosynthesis by exogenous 5-aminolevulinic acid in an aerobic photosynthetic bacterium, erythrobacter sp. och 114.	exogenously administered 5-aminolevulinic acid (ala) inhibited the formation of bacteriochlorophyll a (bchl a) in a dose-dependent manner in the aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, under dark growth conditions. the ala concentration required for half-inhibition after 24-h growth was estimated to be about 3.0 mm. porphyrin and bchl precursors were not found in either the cells or the growth medium. the same inhibition was also observed with cytochrome c formation. ...	1988	2847654
isolation and characterization of a specific receptor for human albumin on a group l streptococcus.	certain group l streptococci demonstrate surface receptors for human albumin. binding of 125i-albumin to group l streptococci could be inhibited by unlabelled albumin preparations from humans, dogs, mice and bovines, but not by albumin from rabbits. the albumin-binding proteins (abp) could be solubilized from the streptococcal surface by hot acid treatment of the bacteria and isolated by affinity chromatography on human-albumin sepharose. abp and specific antisera produced against abp inhibited ...	1988	2848386
transcriptional and translational events during reovirus infection.	this short review focuses on the mechanisms involved in transcription and translation in mouse l cells infected with reoviruses. the viral genomic rna (double-stranded), retained in the inner capsid following removal of the outer capsid of the infecting virion, is transcribed by a viral polymerase. the synthesized viral mrna is blocked at the 5' end by a cap structure similar to the cap structure of cellular mrna but synthesized by the viral enzymes of the inner capsid. this viral mrna is also u ...	1988	2848543
conjugation in saccharomyces cerevisiae.		1988	2848554
sensitive elisa for the gp120 and gp160 surface glycoproteins of hiv-1.	we have used a panel of polyclonal and monoclonal antibodies against gp120 and gp160, the envelope glycoproteins of human immunodeficiency virus type 1, to create rapid, simple, and sensitive twin-site sandwich elisa specific for gp120 and gp160 or for gp160 alone. these assays can detect 500 cos cells in a population transiently transfected with a construct encoding gp120 and gp160, or 50 pg of recombinant gp160. we estimate that the mean amount of gp120 + gp160 in the transfected population is ...	1988	2848557
the role of diffusible molecules in regulating the cellular differentiation of dictyostelium discoideum.	a central problem in developmental biology is to understand how morphogenetic fields are created and how they act to direct regionalized cellular differentiation. this goal is being pursued in organisms as diverse as moulds, worms, flies, frogs and mice. each organism has evolved its own solution to the challenge of multicellularity but there appear to be common underlying principles and, once pattern formation is fully understood in any system, some general truths seem certain to be revealed. a ...	1988	2848678
enzymatic introduction of a fluorescent sialic acid into oligosaccharide chains of glycoproteins.	aiming at the introduction of a fluorescent sialic acid into glycoconjugates, 5-acetamido-9-(3-fluoresceinylthio-ureido)-3,5,9-trideoxy-2-non ulosonic acid (9-fluoresceinyl-neuac) was synthesized which has an intact carbon chain. a) despite the space-filling substituent at c-9, the fluorescent neuac analogue was activated to the corresponding cmp-glycoside by cmp sialic acid synthase from bovine brain. whereas the km value of the synthase was little affected by the modification (km = 2.1 mm, for ...	1988	2848703
transcriptional regulation of the gene encoding the major surfactant protein (sp-a) in rabbit fetal lung.	the expression of the major protein of rabbit pulmonary surfactant (sp-a), a glycoprotein of mr congruent to 29,000-36,000, is regulated during development and by hormones. in the present study, utilizing a cdna insert complementary to mrna coding for sp-a and nuclear transcription elongation assays, we have investigated the developmental and hormonal regulation of transcription of the sp-a gene in rabbit fetal lung tissue. the relative rates of transcription of sp-a mrna increased as a function ...	1988	2848814
epidermal growth factor-stimulated dna synthesis requires an influx of extracellular calcium.	the dependency of normal cell proliferation on adequate extracellular ca2+ levels was further investigated by determining the role of ca2+ influx in epidermal growth factor (egf)-induced rat liver epithelial (t51b) cell dna synthesis. fura-2-loaded t51b cells responded with an increase in [ca2+]i to egf (5-50 ng/ml) that was blocked by low (25 microm) extracellular ca2+ or by pretreatment with 50 microm la3+ to inhibit plasma membrane ca2+ flux. confluent t51b cells treated for 24 h with egf (0. ...	1988	2851595
glomus tumor of the trachea. immunohistochemical and electron microscopic studies.	glomus tumor of the trachea which developed in a 51-year-old man was reported. he experienced frequent episodes of respiratory infections and hemoptosis for 9 months. bronchofiberscopic study demonstrated a polypoid tumor in the upper trachea. local resection of the tumor was performed and it arised from the membranous portion of the third and fourth tracheal rings. histologically, the tumor was composed of medium-sized rounded cells, resembling carcinoid tumor. desmin and myosin immunoreactivit ...	1988	2851778
[changes in the cyclic nucleotide system of t-lymphocytes in experimental tuberculosis in animals].		1988	2851783
phorbol ester and dibutyryl cyclic amp reduce content and efflux of taurine in primary cerebellar astrocytes in culture.	in 16-18 days in vitro (div) primary astrocyte cultures prepared from 7- to 9-day-old rats, 48 h exposure to 12,13-phorbol dibutyrate (pdbu) (1 microm) or dibutyryl camp (dbcamp) (1 mm) reduced cellular taurine content, and both basal and 50 mm k+-evoked taurine efflux, but did not alter cellular glutamate or total protein content. decreases in cellular taurine content first became apparent between 1 and 6 h and were maximal after 24 h. treatment also rapidly altered astrocyte morphology to a mo ...	1988	2852322
a novel peptide vasoconstrictor, endothelin, is produced by vascular endothelium and modulates smooth muscle ca2+ channels.	since the discovery of endothelium-dependent vasodilation, vascular endothelium has been recognized as an important functional unit contributing to the regulation of vascular tonus. recent purification, structure determination and molecular cloning of a novel peptidergic vasoconstrictor, endothelin, from the culture supernatant of porcine aortic endothelial cells has further substantiated this concept. starting from a large quantity of serum-fee endothelial cell-conditioned medium, endothelin wa ...	1988	2853725
phenyl-t-butylnitrone, a free radical spin-trapping agent, inhibits acetylcholine-induced relaxation and cyclic gmp accumulation in rat thoracic aorta.		1988	2854109
establishment and characterization of an epstein-barr virus negative b-cell lymphoma cell line and successful heterotransplantation.	an ebv-negative b-cell lymphoma cell line designated as hbl-1 was established from the pleural effusion of a patient with malignant lymphoma, diffuse, large cell. surface marker studies revealed that hbl-1 cell possessed the same phenotypes of a mature b-cell as the original lymphoma cell from pleural effusion. hbl-1 closely resembled morphologically to the original lymphoma cells observed in the lymph node biopsy and pleural effusion. chromosome analysis revealed that hbl-1 cells showed chromos ...	1988	2854303
in vitro activity of azithromycin (cp-62,993), a novel macrolide, against enteric pathogens.	azithromycin (cp-62,993 [9-deoxy-9a-methyl-9a-aza-9a-homoerythromycin]) is a novel macrolide antimicrobial. in this study the in vitro activity of cp-62,993 has been determined against selected enteropathogens, including clostridium difficile, and compared with that of erythromycin. mics were determined using an agar incorporation technique in mueller-hinton medium, containing saponin-lysed horse blood at a final concentration of 10% v/v, with an inoculum of 10(4) cfu. cp-62,993 was considerably ...	1988	2854515
human endometrial proteins.	human endometrium synthesizes a number of proteins. two of the major proteins are the low molecular weight insulin-like growth factor-binding protein (igf-bp25) and endometrial protein pp14 (placental protein 14). both proteins have been cloned from human decidual cdna library and their complete amino acid sequences have been deduced. igf-bp25 is found in various tissues and body fluids including secretory and decidualized endometrium, amniotic fluid, liver, follicular fluid and luteinized granu ...	1988	2854652
outer membrane permeability in pseudomonas cepacia: diminished porin content in a beta-lactam-resistant mutant and in resistant cystic fibrosis isolates.	since beta-lactam resistance is a feature of pseudomonas cepacia isolates causing pulmonary infections in cystic fibrosis (cf), this study was undertaken to determine whether alterations in beta-lactam permeability mediate drug resistance in this species. a beta-lactam-susceptible non-cf isolate (strain 75-26), a resistant mutant derived from 75-26 by selection for cross-resistance to ciprofloxacin and ceftazidime, and two resistant cf isolates of p. cepacia were used. permeability constants wer ...	1988	2855296
role of glycosyl phosphoinositides in insulin action.		1988	2855505
ph-dependent accumulation of the vesicular stomatitis virus glycoprotein at the ends of intact virions.	the electron microscope was employed to examine the effect of ph on the distribution of the vesicular stomatitis virus glycoprotein (vsv g-protein) on the surface of intact virions. both rotary shadowed and negatively stained preparations demonstrated that whereas at neutral ph the glycoprotein was evenly distributed along the virus membrane, at ph 5.5 it was concentrated at the ends giving the overall virion a distinct bilobed or bipolar morphology. negatively stained specimens showed that the ...	1988	2849241
expression of env sequences of the bovine leukemia virus (blv) in the yeast saccharomyces cerevisiae.	dna sequences of the envelope (env) gene of the bovine leukemia virus (blv) were expressed in the yeast saccharomyces cerevisiae. two yeast promoters, the repressible pho5 promoter and the constitutive pgk promoter, were used to construct four expression plasmids comprising either a sequence of the surface antigen gp51 or a (gp51 + gp30) sequence. the expressed heterologous gene products were characterized by western blot analysis and competitive radioimmunoassay. by means of northern blot analy ...	1988	2849260
[the relationship between transport site of hpd and photodamage region of human gastric cancer cells in the cell cycle].		1988	2849266
post-epizootic surveys of waterfowl for duck plague (duck virus enteritis).	surviving birds from nine duck plague outbreaks in urban and confined waterfowl were sampled for duck plague (dp) virus and dp antibody during 1979-86. duck plague virus was found in combined oral and cloacal swabs of birds from three outbreaks, and dp-neutralizing antibody was demonstrated in some birds from all nine outbreaks. greater prevalence of dp antibody and higher titers were found in survivors from confined populations than from free-flying urban populations. free-flying waterfowl from ...	1988	2849402
retention enhancement by pre-test beta-endorphin and oxotremorine and its reversal by scopolamine.	the pre-test administration of beta-endorphin (0.05 microgram/mouse), oxotremorine sesquifumarate (5.0 micrograms/mouse), or beta-endorphin plus oxotremorine enhanced retention test performance of a conditioned emotional response in mice. the effects were blocked by scopolamine. scopolamine had no effect of its own. the data suggest that the retention enhancement caused by beta-endorphin may involve the secondary activation of cholinergic mechanisms.	1988	2849408
salience and the effects of cs preexposure on aversion conditioning.	rats (n = 84) received preexposure to distilled water or to one of two differently salient flavors, 5.0% casein or 10.0% sucrose, casein being the more salient. each preexposure group then received aversion conditioning to a 5.0% casein or a 10.0% sucrose cs. aversion effects were reliably more enduring to casein than to sucrose. relative to water-preexposed groups, preexposure to casein attenuated aversion effects to the casein cs reliably less than preexposure to sucrose attenuated aversion ef ...	1988	2849411
plasma from uninephrectomized rats stimulates production of inositol trisphosphates and inositol tetrakisphosphate in renal cortical slices.	metabolism of inositol phosphates in renal cortical slices was investigated in vitro after addition of plasma from uninephrectomized or sham-operated rats. plasma from uninephrectomized rats stimulated production of insp3 (inositol trisphosphate) when obtained within the first 3 h after uninephrectomy. with different amounts of added plasma a graded response of insp3 production was obtained. this effect could be prevented by 0.1 microm-tpa (12-o-tetradecanoylphorbol 13-acetate). when analysis of ...	1988	2849423
properties and developmental regulation of the protein phosphatases in dictyostelium discoideum.	the properties and developmental regulation of the protein phosphatases of dictyostelium discoideum were examined. when crude extracts from vegetative cells were separated on a mono q column (fplc) three protein phosphatase peaks, designated p1, p2 and p3 were found. when aggregation and culmination cells were examined only one protein phosphatase peak was observed. this corresponded to phosphatase p1 of vegetative cells. all three of the vegetative cell phosphatase were inhibited by heparin and ...	1988	2849442
muscarinic receptor coupling to inositol phospholipid metabolism in guinea-pig cerebral cortex, parotid gland and ileal smooth muscle.	inositol phospholipid hydrolysis induced by agonist-stimulation of muscarinic receptors has been examined in slices of guinea-pig cerebral cortex, parotid gland and ileal smooth muscle. an assay measuring 3h-inositol phosphate formation from prelabelled lipids in the presence of licl, allowed marked stimulation by agonists to be followed. the pd2-value of carbachol differed markedly, between tissues being more than 10-fold lower in cerebral cortex than in parotid gland. the partial agonist oxotr ...	1988	2849446
a new class of free radical scavengers reducing adriamycin mitochondrial toxicity.	beef heart mitochondria were incubated with adm and nadh. an adriamycin semiquinone radical was detected using esr spectroscopy. the semiquinone radical production rate is decreased upon addition of a scavenger (ad 20) in the reaction medium. nmri mice were treated with ad 20 (70 mg/kg, i.p.) 15 min prior adm injection (20 mg/kg, i.p.) or with adm alone. heart mitochondria were isolated 48 hr later. the enzymatic activities of complex i-iii and complex iv of the mitochondrial respiratory chain w ...	1988	2849451
kininase ii from human seminal plasma. isolation and properties.	a rapid and highly efficient procedure for purification of kininase ii from human seminal plasma is described. after ultra centrifugation, the enzyme was purified by gel filtration on sepharose 6b cl and ion exchange chromatography followed by affinity chromatography of edta-inhibited enzyme on bradykinin-sepharose. the enzyme was specifically inhibited by captopril and bpp9a but not by phosphoramidon. page in the presence of sodium dodecyl sulfate under reducing conditions resulted in two major ...	1988	2849459
[immunologic diagnosis of cytomegalovirus infections in patients after kidney transplantation].		1988	2849513
avian nephroblastomas induced by a retrovirus (mav-2) lacking oncogene. ii. search for common sites of proviral integration in tumour dna.	to demonstrate the existence of a common site of integration in independent tumour clones, restriction mapping of the vicinity of integrated mav-2 proviruses in nephroblastoma dna was performed, using southern hybridization with an mav-2 specific probe u3(pat). the results have shown that (1) nephroblastomas are of semiclonal origin. (2) nephroblastoma cells contain an average of 5 clonally located integrated proviruses per diploid genome; they do not contain any detectable amount of non-integra ...	1988	2849568
suppression of sv40-promoted gene expression by differentiation of preadipose cells.	when a plasmid bearing the chloramphenicol acetyltransferase (cat) gene under the control of an sv40 early promoter is introduced into preadipose or adipose cells of line 3t3-f442a, the promoter directs high levels of transient expression of cat. however, when the plasmid is introduced into preadipose cells and the cells are then allowed to differentiate into adipose cells, the expression of the cat gene is suppressed. in this process, the plasmid is not changed detectably in amount, topology, o ...	1988	2849579
effect of na+/h+ exchange inhibitors on agonist-induced contraction of rat aorta.	a number of vasoconstrictor agonists activate the na+/h+ antiport system in vascular smooth muscle, leading to alkalinization of the cytosol and influx of na+. it is believed that agonist-induced na+/h+ exchange may play an important role in contraction. we have evaluated this hypothesis by determining the effect of inhibition of na+/h+ exchange on phenylephrine (pe)-induced contraction of rat aorta. preincubation of rat aorta with the na+/h+ exchange inhibitor amiloride (0.5 mm) inhibited subse ...	1988	2849662
protein kinase c activities are increased in rat thyroid epithelial cells expressing v-ras genes.	both cytoplasmic and membrane-bound protein kinase c activities are increased in: harvey-sarcoma virus, infected thyroid epithelial cells. the cytoplasmic kinase c increase is found in the chromatographic fraction eluted at lower salt concentration (100 mm nacl-s100), while the more acidic protein fraction eluted at higher salt concentration (35 mm nacl-s350) is virtually absent. although the cytoplasmic s100 fraction from the control and ras-virus infected cells display a comparable pbt2 bindin ...	1988	2849929
lipid-dependent membrane enzymes. purification to homogeneity and further characterization of diacylglycerol kinase from escherichia coli.	1. diacylglycerol kinase apoprotein was purified from membranes of escherichia coli k12 by a six-step procedure that included hplc. the proposed assignment of the enzyme to the dgka gene [lightner et al. (1983) j. biol. chem. 258, 10856-10861] could be supported by molecular mass determination (approximately 14 kda), n-terminal sequencing (met-ala-asn), cyanogen bromide fragmentation and amino acid analysis. as predicted, proline was absent. 2. the membrane-associated as well as the butan-1-ol-d ...	1988	2828054
a comparison of pcp-like compounds for nmda antagonism in two in vivo models.	the pcp-like compounds ketamine and dexoxadrol were evaluated in two behavioral test procedures known to be sensitive to competitive n-methyl-d-aspartate (nmda) receptor antagonists. in the nmda-induced convulsion test in mice, ketamine and dexoxadrol blocked convulsant activity only at doses that also induced nonspecific effects of pcp-like behaviors, thereby confounding the interpretation of results. these compounds also blocked nmda-induced discriminative stimuli in rats; however, this effect ...	1988	2828792
inhibition of rat brain adenylate cyclase activity by benzodiazepine through the effects on gi and catalytic proteins.	the effect of benzodiazepines on adenylate cyclase system was examined in rat brain. micromolar concentrations of diazepam inhibited the enzyme activity in synaptic membranes in dose- and time-dependent manners. the inhibitory effect of diazepam was more evident on the enzyme activity in the presence of guanylyl-5'-imidodiphosphate (gppnhp) or naf-alcl3 than on that in the basal state. in the pertussis toxin-treated membranes, the effect of diazepam in the presence of gppnhp or naf-alcl3 was mar ...	1988	2828793
differential effects of benzodiazepines on phospholipid methylation in hippocampus and cerebellum of rats.	to elucidate the relationship between the occupancy of bdz binding sites and phospholipid methylation in brain, we examined phosphatidylethanolamine-n-methyltransferase (pemt) activity in synaptosomes of rat hippocampi and cerebella in the presence of bdz ligands with different modes of action. we found that ro 5-4864, a specific ligand for "peripheral type" receptors, increased pl methylation in hippocampal and cerebellar synaptosomes. this effect was directly related to receptor occupancy, sin ...	1988	2828796
herpes simplex virus infection of the hand. a profile of 79 cases.	seventy-nine cases of herpes simplex (hsv) infection of the hand were documented by viral culture over a 40-month period. a marked bimodal age distribution was found with 32 cases occurring in adults aged 21 to 30 years and 16 occurring in children less than 10 years old. the infection most commonly occurred on the fingers (69 percent) and thumb (21 percent). all 20 herpes simplex type 2 (hsv-2) isolates came from patients over 20 years old. all of the 13 specimens that were typed after isolatio ...	1988	2827469
investigation of opioid receptor binding protein in adult and fetal mouse brain membranes by photoaffinity labeling.	125i-[d-ala2,p-n3-phe4-met5]enkephalin was used to investigate the opioid receptor from brain membranes of adult (6 months old) and fetal (16 days gestation) mice. the photolabeled membranes were analyzed by sodium dodecyl sulfate gel electrophoresis. a 46,000-da protein was specifically photo-labeled in the adult mouse brain membranes. the photolabeling of this protein was inhibited in the presence of [d-ala2,met5]enkephalin and a peptide ligand specific for the mu-opioid receptor. no specific ...	1988	2829063
cloning and expression of the vibrio cholerae neuraminidase gene nanh in escherichia coli.	a cosmid gene bank of vibrio cholerae 395, classical ogawa, was screened in escherichia coli hb101 for expression of the vibrio neuraminidase (nanase) gene nanh (n-acylneuraminate glycohydrolase). positive clones were identified by their ability to cleave the fluorogenic nanase substrate 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid. seven nanase-positive clones were detected after screening 683 cosmid isolates with a rapid, qualitative plate assay method. the nanh gene was subcloned ...	1988	2832365
characterization and mapping of the agrocinopine-agrocin 84 locus on the nopaline ti plasmid ptic58.	overlapping segments of ptic58 inserted into cosmid vectors were used to characterize the agrocinopine-agrocin 84 locus from the nopaline/agrocinopine a and b agrobacterium tumefaciens strain c58. all of the clones conferring agrocin 84 sensitivity on agrobacteria also conferred uptake of agrocin 84 and agrocinopines a and b. transposon tn3-hoho1 insertion mutations of one such clone were generated that simultaneously abolished agrocin 84 sensitivity and transport of agrocinopines a and b and ag ...	1988	2832379
glucose and carbachol generate 1,2-diacylglycerols by different mechanisms in pancreatic islets.	diacylglycerols (dag) modulate secretory responses by the activation of protein kinase c. early changes in dag formation induced by the muscarinic receptor agonist carbachol were compared to those caused by the nutrient secretagogue glucose in pancreatic islets. turnover rates of dag were investigated in radiolabeling experiments, whereas changes in total mass and fatty acid composition of dag were assessed by gas-liquid chromatography. when islet lipids were labeled to steady state in tissue cu ...	1988	2832445
isolation of a simian virus 40-simian agent 12 recombinant papovavirus that synthesizes a hybrid large tumour antigen.	the simian virus 40 (sv40) mutant dl1055 carries a 55 base pair deletion in the viral early region coding sequences that causes premature termination of large tumour (t) antigen translation resulting in a protein fragment consisting of the amino-terminal 399 residues. the mutation renders the virus defective. we report the characterization of hyb4001, which was isolated as a single plaque following cotransfection of permissive cells with dl1055 dna and the dna of a related papovavirus, simian ag ...	1988	2832530