Publications

australian fraud inquiry under way.		1988	3386761
[the occurrence of parasites in the bream (abramis brama l.), roach (rutilus rutilus l.) and perch (stizostedion lucioperca l.) in the włocławek dam reservoir on the vistula river].		1988	3388868
chemical synthesis of deuterated folate monoglutamate and in vivo assessment of urinary excretion of deuterated folates in man.	the synthesis and in vivo application of stable-isotopically labeled folic acid was investigated to devise methods suitable for studies of folate metabolism in human subjects. glutamate-labeled tetradeutero-pteroylglutamic acid (d4-folic acid) was prepared by mixed anhydride coupling of n10-trifluoroacetylpteroic acid and dimethyl l-[3,3,4,4-2h4]glutamic acid, saponification in sodium deuteroxide, and chromatographic purification. retention of the isotopic label was verified by proton nmr and ma ...	1988	3389521
[study of the effect of antitumor agents on cell cycle traverse by flow cytometry].	the effect of antitumor agents on the cell cycle traverse of cultured fl cells, a monolayer culture line derived from human amnion, was investigated by flow cytometry (fcm) in combination with simultaneous determination of total cell number, viability, mitotic index and labeling index by [3h]tdr. the results obtained can be summarized as follows: antimetabolites prolonged the duration of the s phase at a low dose, while at a high dose they decreased the rate of progression from the g1 to s phase ...	1988	3389824
new orally active alpha-ketohydroxy pyridine chelators for the treatment of iron overload.		1988	3390580
morphology and contractile properties of smooth muscle cells isolated from the dog carotid artery.	collagenase and elastase treatment was used to isolate vascular smooth muscle cells from canine carotid artery. their structure and function were compared to those in situ. morphological studies showed that these cells when relaxed in situ were 120-133 micron mean length, connected by numerous typical gap junctions, covered by a basal lamina and like other smooth muscles in structure. after isolation, the median length of single cells was 82 micron. there was structural evidence of some contract ...	1988	3390615
quantitative carcinogenesis in relation to human exposures.	while carcinogenic potency cannot be expressed in absolute terms, quantitative carcinogenesis nevertheless plays an important role both in assessing cancer risks and in devising effective methods of cancer prevention. attention is drawn to newer methods of prevention based on interference with the carcinogenic process, which is likely to counterbalance, or even to reverse, the anticipated trend of increased cancer incidence resulting from new carcinogens introduced in our environment.	1988	3390839
differences between serum and urinary lh in hypergonadotropic states.	in an attempt to investigate the relationship between molecular configuration, immunoreactivity, radioligand binding, and biological activity, we compared the elution profiles of immunoreactive and radioligand receptor-active lh following gel filtration over sephadex g-100 (1.6 x 100 cm column). samples of sera and urinary acetone-insoluble material from normal cycling women during the lh surge (n = 4) and postmenopausal (n = 2) and castrate women (n = 2) were examined. one major peak of lh immu ...	1988	3391331
comparison of apha and elevated temperature enrichment methods for recovery of vibrio cholerae from oysters: collaborative study.	two methods (the american public health association (apha) method and the elevated temperature method) for recovery of vibrio cholerae from oysters were compared in a collaborative study. oysters were inoculated with high (about 150 cells/g) and low (about 20 cells/g) levels of v. cholerae. the elevated temperature method gave a significantly higher (p less than 0.05) recovery rate and showed greater specificity (p less than 0.01), as determined by the confirmation rate of suspect colonies. the ...	1988	3391968
field trial of oral cholera vaccines in bangladesh: results of one year of follow-up.	we assessed the protective efficacy (pe) of three doses of b subunit-killed whole cell (bs-wc) and killed whole cell-only (wc) oral cholera vaccines in a randomized, double-blind trial among 62,285 children and women residing in rural bangladesh. after one complete year of surveillance, 110 cases of cholera were detected in the placebo group, 52 in the wc group (pe, 53%; p less than .0001), and 41 in the bs-wc group (pe, 62%; p less than .0001). protection was greater for bs-wc recipients than f ...	1988	3392421
dissociation of hcg-receptor complex and detection of hcg receptors in human chorionic tissues.	we developed a radioreceptor assay (rra) system for investigating occupied hcg receptors by using a rat testis membrane fraction. treatment with dithiothreitol, neuraminidase, and low ph (3.3) buffer partially dissociated hcg from its receptor with dissociation rates of 42.8%, 35.2%, and 68.7%, respectively. specific receptor rebinding was unaffected and showed competitive inhibition of [125i]hcg rebinding by unlabeled hcg. scatchard plot analysis yielded a rebinding km of 1.798-3.496/pm. high c ...	1988	3392440
[the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase].		1988	3395180
glycoconjugate expression in human renal oncocytomas. a lectin histochemical study.	a panel of lectins chosen for its specificity for segments of the nephron was used in an avidin-biotin-peroxidase reaction to stain 13 human renal oncocytomas and a specimen of oncocytomatosis. the lectins were derived from lotus tetragonolobus, glycine max, dolichos biflorus, and arachis hypogaea. two patterns predominated: one of staining with the lectins of d biflorus and g max, suggesting distal tubular or collecting-duct differentiation, and one of staining with the lectin of l tetragonolob ...	1988	3395218
establishment and characterization of a doxorubicin-resistant human bladder cancer cell line (mgh-u1r).	a doxorubicin-resistant bladder cancer cell line has been established. this was accomplished by exposing an established human bladder tumor cell line, mgh-u1, to progressively higher concentrations of doxorubicin over a period of 12 months. the resistant cells, mgh-u1r, are nine times more resistant to doxorubicin and 30 times more resistant to daunorubicin than the parent cells. the mgh-u1r and the mgh-u1 cells have identical isozyme phenotypes. compared to the parent cells, the resistant cells ...	1988	3398164
the vascularization of the peripheral nerve of chicken and rat.	the vascular system of the sciatic nerve of chicken and rats was examined by means of the microcorrosion casting technique and freeze-broken specimens. the main epineural vessels form two lateral and interfascicular vascular bundles which anastomose with one another and also with the peri- and endoneural plexuses. on epi- and perineural vessels one can find morphological correlates for regulative means such as sphincters. even the endoneural vessels depict numerous anastomoses. the proximity of ...	1988	3399842
influence of f strain mycoplasma gallisepticum infection on response of commercial layers to heat exposure.	commercial layers were inoculated with f strain mycoplasma gallisepticum (mg) and housed in either conventional chicken houses or the lower-stress environment of biological isolation units. at the end of 2 weeks, all treatment groups were placed in environmental chambers and subjected to 4 hr of heat stress (40 c with a dew point of 21 c). rectal temperature, an indicator of response to high heat, was monitored. rectal temperatures of f strain mg-inoculated hens housed in the conventional chicke ...	1988	3401170
structure of phosphate-free ribonuclease a refined at 1.26 a.	the structure of phosphate-free bovine ribonuclease a has been refined at 1.26-a resolution by a restrained least-squares procedure to a final r factor of 0.15. x-ray diffraction data were collected with an electronic position-sensitive detector. the final model consists of all atoms in the polypeptide chain including hydrogens, 188 water sites with full or partial occupancy, and a single molecule of 2-methyl-2-propanol. thirteen side chains were modeled with two alternate conformations. major c ...	1988	3401445
isolation of a cdna for rat brain glutaminase.	a single phage was isolated from a lambda gt11 rat brain cdna library by screening with antibodies prepared against rat renal glutaminase. partial proteolysis of the fusion protein produced by a lysogen of the isolated phage generated a series of immunoreactive peptides that co-migrated with those derived from the purified brain glutaminase. the cdna has a single open reading frame which encodes 326 amino acids that are in frame with beta-galactosidase. a 72-kda protein, corresponding in size to ...	1988	3401701
[non-o1 vibrio in acute diarrhea of the infant].	the choleriform diarrhoea may be caused by vibrio cholerae, but also by other vibrionaceae exhibiting the cholera-toxin antigenic determinants. the authors report three instances of gastroenteritis in infants, caused by 3 strains of non-o1 vibrio and they carry out bacteriological study on these strains and their pathogenicity-strength factors.	1988	3402007
flow cytometric analysis of the phenotypic changes in tumour cell lines following tpa induction.	single-cell analysis by flow cytometry has enabled us to analyze the effects of a phorbol ester and known tumour promoter, tpa, on the phenotypes of four tumour lines. tpa is capable of triggering a variety of cellular alterations that can affect gene expression and the biochemical balance of intracellular events. we have investigated the effect of tpa on such properties as rate of proliferation, differentiation, expression of cell surface molecules, and susceptibility to natural killer (nk) cel ...	1988	3402283
alveolar macrophages in rabbits after combined exposure to nickel and trivalent chromium.	rabbits were exposed to a combination of 0.7 mg/m3 ni2+ as nicl2 and 1.2 mg/m3 of cr3+ as cr(no3)3, to 0.6 mg/m3 of ni2+ as nicl2, or to filtered air for about 4 months, 5 days/week and 6 hr/day. alveolar macrophages were recovered by lung lavage and studied by light and electron microscopy. metabolic activity, phagocytic capacity and lysozyme activity in the macrophages were studied. after the combined exposure, the effects on lung weight, number of macrophages, and appearance of surface and nu ...	1988	3402403
anabolic effects on rate, composition and energetic efficiency of growth in cattle fed forage and grain diets.	the effects of anabolic implants on rate, composition and energetic efficiency of growth were determined in steers fed diets varying in forage and grain content. santa gertrudis-cross steers averaging 337 kg were group-fed (n = 72) or individually fed (n = 45) ad libitum one of three diets and either not implanted or implanted (90-d intervals) with ralgro or synovex-s implants. steers were fed to a similar empty body weight (463 kg). initial empty body composition of individually fed steers was ...	1988	3403412
phagocytic and nitroblue tetrazolium reductive properties of bovine neutrophils for mammary pathogens.	a comparative evaluation of in vitro phagocytic and metabolic activities of freshly isolated bovine blood neutrophils from 26 cows was performed. degrees of phagocytosis and nitroblue tetrazolium reduction by neutrophils were measured using zymosan, escherichia coli, staphylococcus aureus, streptococcus agalactiae, mycoplasma bovis, salmonella sp., and brucella abortus opsonized with fresh bovine serum. percent phagocytosis and nitroblue tetrazolium reduction by the neutrophils were determined b ...	1988	3403757
the complete nucleotide sequence of potato virus x and its homologies at the amino acid level with various plus-stranded rna viruses.	double-stranded cdna of potato virus x (pvx) genomic rna has been cloned and sequenced. the sequence [6435 nucleotides excluding the poly(a) tract] revealed five open reading frames (orfs) which were numbered one to five starting at the 5' terminus of the rna. they encoded proteins of mr 165588 (166k), 24622 (25k), 12324 (12k), 7595 (8k) and 25080 (coat protein), respectively. orfs 1 and 2 were inphase coding regions. the orf 1 product contained domains of homology with the tobacco mosaic virus ...	1988	3404114
[sensitivity of vibrio cholerae to the lethal and mutagenic effects of uv-irradiation mediated by the presence of plasmids].	the effect of uv-irradiation on vibrio cholerae cells and its changes mediated by the plasmid r245 have been studied. vibrio cholerae strains 569b and rv31 have been shown to be considerably more sensitive to lethal effect of uv-irradiation as compared with escherichia coli and salmonella typhimurium cells. highly toxigenic strain 569b and practically atoxigenic strain rv31 have the same uv-sensitivity. lethal effect of uv-irradiation on vibrio cholerae cells is increased when the irradiated cel ...	1988	3405234
pregnancy resulting from cryopreserved human embryos using a one-step in situ dilution procedure.	in vitro fertilization and embryo transfer require the use of hormonal manipulation and surgery that may reduce the receptivity of the patient's uterus during the stimulated cycle. cryopreservation of human embryos eliminates the need for immediate transfer, permitting them to be stored until they can be transferred during subsequent unstimulated cycles. embryo cryopreservation is an established procedure in the breeding of laboratory and domestic animals, but has only recently been applied to h ...	1988	3405568
the responsiveness of neurons in the insular gustatory cortex of the macaque monkey is independent of hunger.	(1) in order to determine whether the responsiveness of neurons in the insular gustatory cortex is influenced by hunger, neuronal activity was analysed in it while macaque monkeys (macaca fascicularis) were fed to satiety. the responses of single neurons in the insular gustatory cortex to the protypical taste stimuli glucose, nacl, hcl and quinine hcl, and to fruit juice, were measured before, while, and after the monkey was fed to satiety with glucose or fruit juice. (2) while behavior turned f ...	1988	3406148
effect of bilateral ibotenic acid lesions in the basolateral amygdala on the sham feeding response to sucrose in the rat.	to investigate the role of the basolateral amygdaloid complex in the sham feeding response to sucrose, rats received bilateral ibotenic acid lesions of the basolateral complex and were equipped for sham feeding with implanted chronic gastric cannulas. rats were also tested on different days with 2.5%, 5%, 10%, and 40% sucrose. after 17 hours of food deprivation, bilateral, amygdaloid-lesioned rats sham fed significantly less 2.5% sucrose than sham operated (control) rats. after no deprivation, a ...	1988	3406149
south american brugian filariasis: report of a human infection acquired in peru.	a 27-year-old white woman from new york city acquired an infection by a brugia species while she camped in the amazon basin of peru. she was infected by at least one adult male worm and one gravid female worm. both worms were intact and in a lymphatic vessel of a right cervical lymph node. the lymph node and surrounding fibroadipose tissue contained many microfilariae. the male worm was 50 micron wide and the female, 100 micron. both worms had thin (1 micron) cuticles with fine transverse striat ...	1988	3407837
transient increase of cytosolic free calcium in cultured human vascular endothelial cells by platelet-activating factor.	the effect of platelet-activating factor (paf-acether) on cytosolic free calcium, [ca2+]i, in adherent human vascular endothelial cells in culture was directly determined using a new fluorescent calcium indicator, fura-2. it was found that paf-acether but not lyso paf-acether induced a rapid and transient increase in [ca2+]i in endothelial cells. restimulation with paf-acether after the first challenge did not cause further response, while the cells were able to respond to thrombin. in the absen ...	1988	3408504
late strut fracture of the beall model 105 disc valve prosthesis.	strut fracture, with embolization of the disc occluder, caused the death of a 64-year-old man who had a beall model 105 heart valve prosthesis in the mitral position for 13 years. scanning electron microscopy of the fractured surface revealed evidence of a fatigue failure mechanism in the metal wire. the case is unique in that strut fractures affecting this prosthesis had only been observed previously in the range of 141 to 342 days after implantation. morphological changes in this valve prompte ...	1988	3411991
endotoxemia and cholestasis.	endotoxemia has been incriminated as a major cause of morbidity and mortality in patients with obstructive jaundice. it has been postulated that absence of gastrointestinal bile salt flow in cholestasis enhances portal absorption of bacterial endotoxin from the intestine, thereby predisposing the host to endotoxemia and its complications. this study re-evaluates this pathologic mechanism, using new quantitative chromogenic and conventional qualitative limulus techniques for the detection of bact ...	1988	3413650
a clot-inducing wound covering with high vapor permeability: enhancing effects on epidermal wound healing in partial-thickness wounds in guinea pigs.	although the use of an occlusive wound covering accelerates the reepithelialization of a partial-thickness wound, it has the disadvantage of leading to wound exudate accumulation. the effect of an experimental polyetherurethane (peu) wound covering with a high vapor permeability was compared with an occlusive wound covering (opsite covering) and air exposure with respect to the rate of reepithelialization, eventual epidermal thickness, and scab thickness in 122 partial-thickness wounds in guinea ...	1988	3413682
seroepidemiology of cholera in gulf coastal texas.	single serum samples from 559 volunteers from a texas gulf coast area were examined for vibriocidal antibody to vibrio cholerae o1 (biotype el tor, serotype inaba) by a microtiter method. elevated levels of vibriocidal antibody were present in 14% of the subjects. also, 6.8% of the subjects had elevated levels of antibody to the enterotoxin of v. cholerae o1 by the immunoglobulin g enzyme-linked immunosorbent assay. recent infection, defined on the basis of elevations in both vibriocidal and ant ...	1988	3415232
[morphological bases for the resistance of the intestines to the action of cholera toxoid].	effect of antigen (cholera toxoid) has been studied on distribution of specific antibody-containing plasma cells in the lamina propria of the mucous membrane of the guinea pig small and large intestine. since it is known that experimental cholera is easily reproduced in these animals, amount of plasmocytes is determined after primary intraperitoneal and secondary intraduodenal immunization with cholera toxoid. at the primary immunization maximal increase in amount of plasmocytes is noted in the ...	1988	3415485
[duodenal glands in normal and vagotomized rats].	in wistar rats by means of the stereo- and morphometry methods, using semithin sections, histotopography of the duodenal glands (dg) and effect of vagotomy (vt) on them have been studied. the zone of the dg arrangement in the submucosal tela of the duodenum is about 5,000 mcm long. in the proximo-distal direction of the zone mentioned three parts can be distinguished--oral, middle and caudal. in the dg light-optically four varieties of mucocytes are identified. vt results in decreasing thickness ...	1988	3415497
haemodynamic effects of tiapamil and verapamil in conscious rats following chronic myocardial infarction.	the haemodynamic effects of the two calcium antagonists, tiapamil and verapamil, were compared in conscious rats with or without myocardial infarction. in control rats without myocardial infarction, verapamil was about 20 times more potent than tiapamil to reduce arterial blood pressure. however, if equihypotensive doses of tiapamil and verapamil were compared, verapamil produced a 2-fold larger decrease of dp/dtmax+ (p less than 0.01). three weeks after myocardial infarction, the negative inotr ...	1988	3416326
[clinical disagreement of traditional chinese medicine physicians in treating patients according to syndrome differentiation for chronic persistent hepatitis].		1988	3416394
modified assay for blood coagulation factor xiii with use of antibody for enzyme immobilization.		1988	3416448
electron microscopic study of vibrio cholerae o1 adherence to the mucus coat and villus surface in the human small intestine.	vibrio cholerae o1, irrespective of the biotype or serotype, adhered to and was entrapped in the mucus coat covering the mucosal surface of isolated human ileal segments. the evidence for such mucus coat adherence was obtained by treatment of the ileal segments with 10% formalin. in any case, adherence to the mucus coat was much more prominent than adherence to the epithelial cell surface of the small intestinal villi. mucus coat adherence was affected by sugars and by the growth phase of the ba ...	1988	3417355
genetic characterization of leishmania isolates at 37 enzyme loci.		1988	3417374
cellular and humoral responses of sheep with different levels of resistance to trichostrongylus colubriformis.		1988	3417380
effect of gamma irradiation on the protoscoleces of echinococcus granulosus of sheep origin.		1988	3417384
effect of gamma irradiation on the protoscoleces of echinococcus granulosus of sheep origin.		1988	3417384
optic disc, cup and neuroretinal rim size, configuration and correlations in normal eyes.	four hundred and fifty-seven unselected normal human optic nerve heads of 319 subjects (163 men, 156 women, mean age 42.7 +/- 19.6 years) were evaluated by magnification-corrected morphometry of optic disc photographs. mean optic disc surface measured 2.69 +/- 0.70 mm2 (0.80-5.54 mm2), mean diameter horizontally 1.76 +/- 0.31 mm (0.91-2.61 mm), and vertically 1.92 +/- 0.29 mm (0.96-2.91 mm). the form was slightly vertically oval. optic cup area averaged 0.72 +/- 0.70 (0.00-3.41 mm2), mean horizo ...	1988	3417404
anatomical organisation of the microvasculature in the anterior and posterior latissimus dorsi muscles of the chicken.	anterior and posterior latissimus dorsi muscles of twelve chickens were injected with india ink to visualise their microvasculature. both muscles have multiple feeding arterioles that branch into a meshwork of arcade arterioles. transverse arterioles arise from the arcade system and these, in turn, give rise to capillaries. the major differences in the microvasculatures of the two muscles are in the numbers of vessels in the arcade meshworks and the dimensions of these vessels. the differences i ...	1988	3417554
bmy-28438 (3,7-dihydroxytropolone), a new antitumor antibiotic active against b16 melanoma. i. production, isolation, structure and biological activity.	a new antitumor antibiotic bmy-28438 was isolated from the cultured broth of streptomyces tropolofaciens no. k611-97. the antibiotic showed potent cytotoxicity against cultured b16 melanoma cells and remarkable prolongation of life span of mice bearing b16 melanoma. the structure of bmy-28438 was determined as 3,7-dihydroxytropolone on the basis of spectral analyses and direct comparison with the authentic compound synthesized.	1988	3417559
crystallization of trimeric recombinant human tumor necrosis factor (cachectin).	crystals of tumor necrosis factor (tnf) have been obtained in two forms. rhombohedral crystals grow in 1.8 to 2.0 m ammonium sulfite, ph 7.8 at 21 degrees c, and tetragonal crystals grow in 2.6 m magnesium sulfate, ph 5.5 at 25 degrees c. analysis of tnf by isoelectric focusing under native and denaturing conditions indicates that tnf molecules exist as trimers in solution. the rhombohedral cachectin crystals belong to space group r3 and have unit cell constants a = b = c = 47.65 a and alpha = b ...	1988	3417634
early postischemic 45ca accumulation in rat dentate hilus.	several studies have found postischemic regional accumulation of calcium to be time-dependent and coincident with the progression of ischemic cell change. in the most vulnerable cells in the hippocampus one would therefore expect to find a primary and specific early uptake of calcium after ischemia. autoradiograms of 45ca and 3h-inulin distribution were investigated before and 1 h after 20 min ischemia in the rat hippocampus. two different methodological approaches were used for administration o ...	1988	3417798
quantification of thermal asymmetry. part 1: normal values and reproducibility.	the use of thermography in evaluating nerve injury is based on the presence of temperature asymmetries between the involved area of innervation and the corresponding area on the opposite side of the body. however, interpretation of the thermographic image has been troubled by subjectivity. this paper describes a computer-calculated method of collecting data that eliminates subjective biases. comprehensive normative data are presented on the degree of thermal asymmetry in the human body. the degr ...	1988	3418388
[an embryological study on the mouse ear using organ culture technic].		1988	3418435
spinal cord penetration of intrathecally administered cytarabine and methotrexate: a quantitative autoradiographic study.	carcinomatous meningitis is an increasingly common complication of systemic cancer. treatment usually involves the use of intrathecal methotrexate or cytarabine, which have been reported to be toxic to the spinal cord. this study was performed to determine the penetration and distribution of intrathecally administered chemotherapy within the spinal cord. tritiated methotrexate and cytarabine were administered intraventricularly and by lumbar puncture to new zealand white rabbits. the extent and ...	1988	3418727
molecular analysis of the packaging signal in bacteriophage cp-t1 of vibrio cholerae.	we have previously identified a unique site, pac, from which packaging of precursor concatameric viral dna into proheads starts during the maturation process of bacteriophage cp-t1. the direction of this packaging was determined from restriction enzyme cleavage patterns of cp-t1 dna. a restriction enzyme generated fragment containing pac was cloned and the surrounding dna region sequenced. analysis of the nucleotide sequence revealed numerous repeat regions related to the consensus sequence puag ...	1988	3419420
common genetic pools between human populations.	a further inquiry on modern human origins, based on common genetic pool surveys of rigorously selected population samples and highly informative immunological polymorphisms, provides new evidence of an occidental-oriental population split as the origin of human gene pool divergences. the most likely ancestral genetic profile is discussed in the context of the debate raised by preliminary dna restriction fragment length polymorphism studies, which contradict the conclusions drawn from classical b ...	1988	3422217
isomeric prostaglandin f2 compounds arising from prostaglandin d2: a family of icosanoids produced in vivo in humans.	prostaglandin (pg) d2 has been shown to be transformed by human 11-ketoreductase to 9 alpha,11 beta-pgf2, a biologically active metabolite that is produced in vivo. during the course of developing a mass spectrometric assay for 9 alpha,11 beta-pgf2, several compounds with characteristics similar to pgf2 were detected in both plasma and urine of normal humans by selected ion monitoring. analysis of pooled plasma obtained from patients with mastocytosis during severe episodes of systemic mast cell ...	1988	3422430
plasmids in bacteria exposed to activated neutrophils mediate mutagenesis when transferred to new hosts.	the plasmid puc18 contains a lacz alpha-complementation gene that codes for a small peptide that can complement the delta m15 mutation of the escherichia coli lacz (beta-galactosidase) gene, converting bacteria carrying that mutated gene from the lacz- to the lacz+ phenotype. this plasmid was used in experiments designed to study mutagenesis by human neutrophils. e coli carrying puc18 were incubated with neutrophils under conditions in which little ingestion of the bacteria took place; the plasm ...	1988	3122860
characterization of the pseudomonas aeruginosa reca gene: the les- phenotype.	the les- phenotype (lysogeny establishment deficient) is a pleiotropic effect of the lesb908 mutation of pseudomonas aeruginosa pao. lesb908-containing strains are also (i) deficient in general recombination, (ii) sensitive to uv irradiation, and (iii) deficient in uv-stimulated induction of prophages. the p. aeruginosa reca-containing plasmid pkml3001 complemented each of these pleiotropic characteristics of the lesb908 mutation, supporting the hypothesis that lesb908 is an allele of the p. aer ...	1988	3123459
inhibition of adenylate cyclase from luteinized rat ovary by monovalent cations: roles of the stimulatory guanine nucleotide-binding regulatory component and stimulatory hormone receptor.	sodium and other monovalent cations (added as chloride salts) inhibited adenylate cyclase of luteinized rat ovary. sodium chloride (150 mm) inhibited basal enzyme activity by 20%. sodium chloride inhibition was enhanced to 34-54% under conditions of enzyme stimulation by guanine nucleotides (gtp and its nonhydrolyzable analog 5'-guanylyl imidodiphosphate), fluoride anion, and agonists (ovine luteinizing hormone (olh) and the beta-adrenergic catecholamine isoproterenol) acting at stimulatory rece ...	1988	3124764
epidermal growth factor, but not thyrotropin, stimulates the expression of c-fos and c-myc messenger ribonucleic acid in porcine thyroid follicle cells in primary culture.	previous studies have shown that tsh stimulates functional parameters in cultures of porcine follicle cells, but has no stimulatory effect on their growth. epidermal growth factor (egf), on the other hand, is a potent mitogen in such cells. the aim of the present investigation was to study the effect of egf and tsh on the expression of proto-oncogene mrna in porcine thyroid follicle cells. the addition of egf caused a marked increase in the c-fos and c-myc mrna levels. stimulation of cells with ...	1988	3125035
effects of methylprednisolone on acute lung paraquat toxicity in sheep.	infused into sheep, paraquat causes increased flow of protein-rich lung lymph, increased prostanoid production, and neutrophil accumulation in the lung. the effects of high-dose methylprednisolone on the response to paraquat infusion were studied in awake sheep with chronic lung lymph fistulas. seven sheep were infused with paraquat (30 mg/kg) alone. six sheep received methylprednisolone (1.0 g plus 0.5 g/h intravenously), beginning 30 min before paraquat (pretreatment), and 5 received methylpre ...	1988	3122611
differential effects of 15-hpete on arachidonic acid metabolism in collagen-stimulated human platelets.	the 15-hydroperoxyeicosatetraenoic acid (15-hpete) has been shown to affect platelet aggregation induced by collagen, arachidonic acid (aa), and pgh2-analogue. furthermore, it also inhibits the platelet cyclooxygenase and lipoxygenase enzymes, and prostacyclin synthase. the present study was designed to test the effect of 15-hpete on the mobilization of endogenous aa in collagen-stimulated human platelets. for this purpose, human platelets pretreated with bw755c (a dual inhibitor of cyclooxygena ...	1988	3122753
an interleukin 2 binding factor in human serum.		1988	3127085
human antibody responses against schistosomal antigens. i. antibodies from patients with ancylostoma, ascaris lumbricoides or schistosoma mansoni infections react with schistosome antigens.	sera of patients with either hookworm infections or ascariasis, who are from areas nonendemic for schistosoma mansoni, contain antibody reactivity against extracts of s. mansoni schistosomula. such patients with hookworm, but not those with ascaris lumbricoides, also express considerable antibody activity against intact schistosomula and schistosomular membrane preparations. sera from patients with schistosomiasis mansoni also contain these activities. because these three helminthic infections o ...	1988	3128128
low trh-tsh responses in human essential hypertension.	thirty female and male essential hypertensive patients and eighteen normotensive controls were submitted to the trh-tsh conventional test (200 ug intravenously, bolus injection of trh). supramaximal doses of 400 and 600 ug were repeated with a week interval to each subject. hypertensives showed a significant lower response to both conventional and supramaximal trh doses. hypothalamic-pituitary-thyroid axis abnormalities, secondary to trh-receptor alterations, could account for this result.	1988	3129219
the omega-hydroxylation of arachidonic acid by human polymorphonuclear leukocytes.	incubations of [1-14c]arachidonic acid with unstimulated human polymorphonuclear leukocytes resulted in the formation of four new metabolites in a previously described reverse-phase hplc system. three of these metabolites were largely suppressed in a co/o2 (80/20, by vol.) atmosphere indicating a cytochrome-p450-dependent monooxygenase reaction. in agreement with this assumption is their nadph/o2-dependent formation in the microsomal fraction. one metabolite was identified by gas chromatography/ ...	1988	3129296
effects of calcium ionophore, a23187 and calmodulin inhibitors on intercellular communication in the rat myometrium.	we have studied the effect of a calcium ionophore, a23187, and the purported calmodulin inhibitors, calmidazolium and chlorpromazine, on direct intercellular communication between smooth muscle cells in the myometrium of delivering rats. the extent of cell-to-cell coupling was determined by exposing one portion of small strips of longitudinal myometrium to 2-[3h] deoxy-d-glucose (2-dg) and determining the distribution and apparent diffusion coefficient (da) for this tracer after a 5-h period for ...	1988	3130110
synergistic activation of human t cells by interleukin 1 and interleukin 6.	purified human interleukin 6 (il 6) was found to stimulate the proliferation of human tonsillar and peripheral rosetting t cells subliminally activated with phytohemagglutinin (pha). this response seemed independent of il 2 but highly dependent on the presence of accessory cells. indeed, when accessory cell-depleted tonsillar t cells were activated with pha and exposed to il 6, only minimal proliferations were observed. a similar result was obtained with il 1. however, a combination of these two ...	1988	3130269
human rabies--california, 1987.		1988	3130561
comparative observations of fever and associated clinical haematological and blood biochemical changes after intravenous administration of vibrio cholerae enterotoxin, staphylococcal enterotoxins and escherichia coli endotoxin in goats.		1988	3130732
anomeric specificity of d-glucose metabolism in human erythrocytes.	the metabolism of d-[5-3h]glucose and d-[u-14c]glucose displays anomeric specificity in human erythrocytes. at close-to-physiological hexose concentrations, pure beta-d-[5-3h]glucose is metabolized at a higher rate than the corresponding pure alpha-anomer in most, but not all, subjects. this situation represents a mirror image of that found in rat erythrocytes. the anomeric preference is modulated by the extracellular concentration of d-glucose and may differ for distinct metabolic variables. th ...	1988	3131049
characterization of prolactin-releasing factor in the rat posterior pituitary.	we previously reported that the rat posterior pituitary contains a potent prl-releasing factor (prf) which is distinct from oxytocin (ot), trh, and angiotensin ii (aii). the objectives of this study were 1) to examine whether posterior pituitary extracts stimulate prl release in the presence of dopamine (da), 2) to determine the chemical nature of prf, and 3) to estimate its mol wt. perifused anterior pituitary cells were used to assess prf activity. posterior pituitaries and medial basal hypoth ...	1988	3131118
gonadotropin-releasing hormone molecular forms in mammalian hypothalamus.	multiple forms of gnrh have been detected in brain tissue of species from all nonmammalian vertebrate classes, but in mammals it is generally believed a single molecular form of gnrh is present. we have investigated the possibility that additional structural variants of gnrh are present in mammalian (sheep, rat, and human) hypothalamus. hypothalami were extracted with acetic acid and subjected to gel filtration chromatography and reverse phase hplc systems specifically designed to separate gnrh ...	1988	3131123
imported human rabies--australia, 1987.		1988	3131653
class switching from gamma 3 to gamma 2b in a murine pre-b cell line.	class switching from gamma 3 to gamma 2b production at pre-b cell stage was observed in an abelson virus-transformed murine immature cell line that is able to perform vh to djh recombinations followed by class switch recombinations. a series of southern blotting experiments indicates that the class switching from gamma 3 to gamma 2b was mediated by the deletion mechanism of the intervening ch genes on the expressed chromosome and that the break points in the switch recombinations might be differ ...	1988	3131665
[control of growth hormone release by growth hormone-releasing factor and prostaglandin e2 in a system of perfused rat anterior pituitary cells].	using superfused rat pituitary cells we showed that the growth hormone-releasing factor (grf) and prostaglandin e2 (pge2) increased growth hormone (gh) secretion in a similar manner in terms of kinetics and amplitude of responses. moreover, our data suggest that they stimulate gh secretion mainly by camp-dependent mechanisms.	1988	3131855
inhibition of 7,12-dimethylbenz[a]anthracene-induced genotoxicity in chinese hamster ovary cells by retinol and retinoic acid.	the effects of retinol and retinoic acid on cytotoxicity and mutation expression at the hypoxanthine-guanine phosphoribosyl transferase (hgprt) locus in chinese hamster ovary (cho) cells with or without exogenous metabolic activation were studied in the presence and absence of known chemical mutagens. neither retinol nor retinoic acid induced mutants at the hgprt locus of cho cells at concentrations ranging from 1 microm to 50 microm without exogenous metabolic activation, and at concentrations ...	1988	3133130
theoretical and practical considerations in the doubly-labelled water (2h2(18)o) method for the measurement of carbon dioxide production rate in man.	in the doubly-labelled water (2h2(18)o) method for the measurement of carbon dioxide production rate in man, single exponential disappearance curves for 2h2o and h2(18)o in body water are used; the precision with which the slopes and intercepts of the curves are estimated determines the precision of the estimate of carbon dioxide production rate. in studies with infants, and in computer simulations, the effect of different experimental regimes on the overall precision of the carbon dioxide produ ...	1988	3133206
selective terminal alpha 2-3 and alpha 2-6 sialylation of glycosphingolipids with lacto-series type 1 and 2 chains in human meconium.	human meconium was found to contain two kinds of gangliosides with the same carbohydrate sequence belonging to the lacto-series. they were detected by tlc-immunostaining with monoclonal antibodies directed to the neuac alpha 2-6gal and lc4cer structures. one of these two gangliosides, a major one, which migrated on tlc to a position below that of standard iv3neuacnlc4cer from human erythrocytes, reacted with the antibody to neuac alpha 2-6gal. the other minor one, which migrated on tlc to a posi ...	1988	3133241
aids as a neuropeptide disorder: peptide t, vip, and the hiv receptor.		1988	3153485
immunobiological relationships between vibrio fluvialis and vibrio cholerae enterotoxins.	a total of 26 strains of vibrio fluvialis was included in this study, which were isolated from patients with diarrhoea and other sources. the gm1 enzyme linked immunosorbent assays performed with the culture filtrates of v. fluvialis yielded negative results, indicating that their receptor site is different from that of the known labile toxin. the cholera antitoxin failed to neutralize the skin permeability factor activities of all the v. fluvialis culture filtrates and none of the concentrated ...	1988	3155161
experimental portal fibrosis produced by intraportal injection of killed nonpathogenic escherichia coli in rabbits.	an attempt was made to develop an animal model for the study of the etiology of noncirrhotic portal fibrosis or idiopathic portal hypertension based on the assumption that it is related to chronic abdominal infection. rabbits were given killed nonpathogenic escherichia coli intraportally or intravenously. the animals to which a mixture of killed e. coli and rabbit antiserum (aggregated e. coli) was given intraportally developed remarkable histologic changes in the liver. the early inflammatory r ...	1988	3276575
in situ survival of vibrio cholerae and escherichia coli in tropical coral reefs.	vibrio cholerae and escherichia coli were inoculated into membrane diffusion chambers and placed around two small coral reef islands in puerto rico and monitored for 5 days. several chambers were also buried in the sands of one of the reefs. both e. coli and v. cholerae densities declined by 2 orders of magnitude, as measured by direct particle counts with a coulter counter (coulter electronics, inc., hialeah, fla.). however, the density of neither bacteria changed dramatically when the same sam ...	1988	3278684
ime1, a positive regulator gene of meiosis in s. cerevisiae.	ime1 (inducer of meiosis) was cloned due to its high copy number effect: it enabled mat insufficient strains to undergo meiosis. disruption of ime1 results in a recessive spo- phenotype. diploids homozygous for the two mutations ime1-0, rme1-1 are also meiosis deficient. we conclude that ime1 is a positive regulator of meiosis that normally is repressed by rme1. rme1 is repressed by a complex of mata1 and mat alpha 2 gene products. ime1 is also regulated by the environment: no transcripts could ...	1988	3280136
ime1, a positive regulator gene of meiosis in s. cerevisiae.	ime1 (inducer of meiosis) was cloned due to its high copy number effect: it enabled mat insufficient strains to undergo meiosis. disruption of ime1 results in a recessive spo- phenotype. diploids homozygous for the two mutations ime1-0, rme1-1 are also meiosis deficient. we conclude that ime1 is a positive regulator of meiosis that normally is repressed by rme1. rme1 is repressed by a complex of mata1 and mat alpha 2 gene products. ime1 is also regulated by the environment: no transcripts could ...	1988	3280136
skin preservation at 4 degrees c: a species comparison.	there are numerous experimental studies in the literature regarding skin storage and preservation. these studies are difficult to interpret due to the variety of storage techniques utilized and the number of different animal species used as skin donors. this study utilized a single cold storage protocol to test the effect of species variation on skin graft viability. donor skin was obtained from five animal species and human surgical panniculectomy specimens. the skin was stored in modified rosw ...	1988	3280246
inducer expulsion in streptococcus pyogenes: properties and mechanism of the efflux reaction.	expulsion of preaccumulated methyl-beta-d-thiogalactoside-phosphate (tmg-p) from streptococcus pyogenes is a two-step process comprising intracellular dephosphorylation of tmg-p followed by rapid efflux of the intracellularly formed free galactoside (j. reizer, m.j. novotny, c. panos, and m.h. saier, jr., j. bacteriol. 156:354-361, 1983). the present study identifies the mechanism and the order and characterizes the temperature dependency of the efflux step. unidirectional efflux of the intracel ...	1988	3280553
inducer expulsion in streptococcus pyogenes: properties and mechanism of the efflux reaction.	expulsion of preaccumulated methyl-beta-d-thiogalactoside-phosphate (tmg-p) from streptococcus pyogenes is a two-step process comprising intracellular dephosphorylation of tmg-p followed by rapid efflux of the intracellularly formed free galactoside (j. reizer, m.j. novotny, c. panos, and m.h. saier, jr., j. bacteriol. 156:354-361, 1983). the present study identifies the mechanism and the order and characterizes the temperature dependency of the efflux step. unidirectional efflux of the intracel ...	1988	3280553
toxoplasmosis and the acquired immune deficiency syndrome.	the literature on toxoplasmosis in the acquired immune deficiency syndrome is reviewed with reference to more than 140 cases from various centres. the incidence, pathogenesis, clinical features, diagnosis, treatment and prognosis are considered.	1988	3280690
dna sequence analysis of spontaneous mutations in the sup4-o gene of saccharomyces cerevisiae.	a collection of 196 spontaneous mutations in the sup4-o gene of the yeast saccharomyces cerevisiae was analyzed by dna sequencing. the classes of mutation identified included all possible types of base-pair substitution, deletions of various lengths, complex alterations involving multiple changes, and insertions of transposable elements. our findings demonstrate that at least several different mechanisms are responsible for spontaneous mutagenesis in s. cerevisiae.	1988	3280976
comparative study of colony hybridization with synthetic oligonucleotide probes and enzyme-linked immunosorbent assay for identification of enterotoxigenic escherichia coli.	on the basis of the published nucleotide sequences of the genes that code for the heat-labile toxin lth and the heat-stable toxins stai and staii of human enterotoxigenic escherichia coli, a 34-mer and two 33-mer oligonucleotide probes were synthesized. to compare their relative efficacies in the detection and differentiation of enterotoxigenic e. coli, a colony hybridization technique using these probes and a gm1 ganglioside enzyme-linked immunosorbent assay using monoclonal anti-lt and anti-st ...	1988	3281978
association of pathogens with the intestinal mucosa and the design of vaccines.		1988	3283533
[epidemiological and ecological problems of el tor cholera].		1988	3284250
[immunodiagnosis of aids using preparations of the recombinant antigen of the surface protein of the htlv-iii virus].	a technological scheme has been developed for purification of recombinant antigen of surface protein (asp) of the causative virus of aids from escherichia coli cells carrying plasmid pl2 which codes for the synthesis of a hybrid polypeptide consisting of phage lambda n protein (59 amino acid residues) and a fragment of sp (env) of hiv virus (569 a.r.). purification of asp is based on two separation principles: fractionation of polypeptides of bacterial lysates by preparative isoelectrofocusing i ...	1988	3285593
vitiligo and the vogt-koyanagi-harada syndrome.	vitiligo is a common acquired systemic disease that can be associated with several different autoimmune disorders. besides the psychologically upsetting depigmentation of the skin that it causes, it can be associated with ocular abnormalities. there are three different theories regarding the etiology of vitiligo, none of which is entirely satisfactory. there are some valuable animal models for studying the disease, but they obviously have limitations in their application to the human disease. th ...	1988	3288383
comparison of the carbohydrate-binding specificities of cholera toxin and escherichia coli heat-labile enterotoxins lth-i, lt-iia, and lt-iib.	the heat-labile enterotoxins of vibrio cholerae and escherichia coli are related in structure and function. they are oligomers consisting of a and b polypeptide subunits. they bind to gangliosides, and they activate adenylate cyclase. the toxins form two antigenically distinct groups; members of each group cross-react but are not necessarily identical. serogroup i includes cholera toxin (ct) and type i heat-labile enterotoxin (lt-i) of e. coli. lth-i and ltp-i are antigenic variants of lt-i prod ...	1988	3290106
improvement of immunofluorescence for diagnosis of aids using laser microscopy.	one of the most commonly used methods for demonstration of hiv antibodies is indirect immunofluorescence employing hiv-infected, cd4-positive lymphoid cell lines as antigenic substrate. immunofluorescence with conventional optic equipment is reported to be slightly less sensitive than enzyme-linked immunoassay (elisa). we have developed an immunofluorescence microscope which is equipped with an argon laser that has the advantages of much brighter fluorescence than conventional techniques, the pr ...	1988	3290334
some properties of membrane current fluctuations induced by kainate, quisqualate, and nmda in cultured septal neurons of rat.	ionic currents induced by glutamate, kainate, quisqualate, and n-methyl-d-aspartate (nmda) in cultured septal neurons were analyzed by fluctuations analysis. the power spectrum (pws) of nmda current fluctuations always fitted a single lorentzian. pwss of the other agonists fitted the sum of two lorentzians; however, the slopes of pwss became larger and the pwss became closer to single lorentzians as the number of drug application increased. this may be explained in such a way that, in multiple c ...	1988	3292010
[structures and biological properties of enterotoxin and hemolysin of non-01 vibrio cholerae].		1988	3294461
effects of growth hormone therapy and malnutrition on the growth of rats with renal failure.	we examined the effects of methionyl-human growth hormone (met-hgh) and malnutrition on the growth of 5/6 nephrectomized rats and sham-operated controls. one group of sham-operated rats (pfs) was pair-fed with a group of nephrectomized rats in renal failure (rf); another group of sham-operated rats was fed ad libitum (als), and a final group of rats with renal failure (rf-gh) was treated with 4 iu/day met-hgh. after 4 weeks, rf-gh rats gained 12.3 +/- 1.7 cm in length; this was more than the 10. ...	1988	3153055
unusual cross-reactions among monoclonal antibodies to bacterial antigens: idiotypic and competitive binding analysis.	in a previous study, we have described unusual cross-reactions among monoclonal antibodies (mabs) to bacteria and in particular to the inaba and ogawa serotypes of vibrio cholerae. in this study, the extent to which the binding sites of both antibodies and antigens overlap has been investigated by competitive binding and idiotypic analysis. the competitive binding data indicate that the cross-reactive binding of the inaba mabs to the ogawa vibrios can be abolished by incubation with higher affin ...	1988	3272825