Publications

the nucleotide sequence for the cdna of bovine interleukin-1 beta.		1988	3262866
vestibular ototoxicity following intratympanic applications of chlorhexidine gluconate in the cat.	our previous studies have shown that the disinfectant chlorhexidine gluconate (ch) has an ototoxic effect on the cochlea following its topical application in the middle ear space. the purpose of the present investigation was to demonstrate morphologically if ch also has the potential to injure the vestibular organs in the inner ear and, if so, what structures are most strongly damaged. nine cats were used for the study. the drug dose of ch and the number of its applications to the middle ear wer ...	1988	3263111
detection of gangliosides of the gm1b type on high-performance thin-layer chromatography plates by immunostaining after neuraminidase treatment.	a method for the detection of gm1b-type gangliosides in complex mixtures of gangliosides was developed. the procedure involves separation of gangliosides on high-performance thin-layer chromatography plates, fixation of the silica gel, treatment with neuraminidase from vibrio cholerae in the absence of detergent, and incubation of the plates with ggose4cer-specific antibodies. alkaline phosphatase-conjugated second antibodies are used to visualize bound first antibodies by generating a blue dye ...	1988	3263817
[isolation and properties of interleukin 1 from human blood monocytes].	among different tested substances lps-containing preparations were found to be most effective inducers of secretory interleukin-1 in human peripheral blood monocytes in vitro. il-1 from supernatant of prodigiozan- + con a-stimulated monocytes had a mm of 18-20 kd and pi of 5.2-5.4 and 6.8 revealing an equal comitogenic activity and of 6.0 (minor peak).	1988	3264189
receptor-mediated endocytosis and degradation of bovine growth hormone in rat liver.	receptor-mediated endocytosis of radiolabelled bovine growth hormone (125i-bgh) via somatogenic receptors in the liver was studied following in vivo intraportal injection. at different times after injection, subcellular membrane fractions involved in binding (plasma membranes), endocytosis (endocytic vesicles) and degradation (lysosomes) of peptide hormones were isolated by sucrose density gradient centrifugation. these fractions were evaluated for the time-course accumulation of radiolabelled b ...	1988	3264255
regulation of type i collagen synthesis by 1,25-dihydroxyvitamin d3 in human osteosarcoma cells.	synthesis of type i and iii collagens has been examined in mg-63 human osteosarcoma cells after treatment with the steroid hormone, 1,25-dihydroxyvitamin d3 (1,25-(oh)2d3). analysis of total [3h]proline-labeled proteins and pepsin-derived collagens revealed that 1,25-(oh)2d3 selectively stimulated synthesis of alpha 1i and alpha 2i components of type i collagen after 6-12 h. consistent with previous reports (franceschi, r. t., linson, c. j., peter, t. c., and romano, p. r. (1987) j. biol. chem. ...	1988	3264282
suppressive and enhancing effect of t-2 toxin on murine lymphocyte activation and interleukin 2 production.	t-2 toxin, a fungal metabolite shown previously to exert potent immunosuppressive effects, was examined for its effects on activation and interleukin 2 (il 2) production by murine and rat splenocytes. splenocytes (1 x 10(6) cells/well) were incubated with 1 microgram concanavalin a (con a) for 48 h at which time cellular protein and dna synthesis by these cells were ascertained using radiolabeled precursors. il 2 synthesis was determined from the cell supernatant using the il 2-requiring cell li ...	1988	3264300
can the case-control method be used to assess the impact of water supply and sanitation on diarrhoea? a study in the philippines.	this study had 2 objectives: 1) to determine whether a case-control approach is an effective measure for assessing the effect of improved sanitation on bacterial diarrhea, and 2) to assess the effects of environmental sanitation on diarrheal disease in cebu. the study took place during the warm, rainy months (july-september) of 1985. the study population consisted of 281 children under 2 who were treated at 1 of 16 clinics for diarrhea due to escherichia coli, salmonella, shigella, and vibrio ...	1988	3264764
partial duplication 14q/deletion 2q in two sibs due to t(2;14) (q37.1;q31.2) pat.	two siblings are described with duplication 14q/deletion 2q due to a paternal translocation (2;14) (q37.1;q31.2). the first one, a boy, born at term, lived 14 days. the second one, a female foetus, was born after induced labour when the anomaly was discovered by way of amniocentesis. they both had almost identical phenotypes. from a study of the literature it is inferred that a typical asymmetric head form, low set abnormal ears, micrognathia, long upper lip, rib anomalies, camptodactyly, long f ...	1988	3265312
age, cellular immunity and the hp axis in major depression.	1. to assess the effect of age on cellular immune status and the hp axis in patients with major depression, we examined peripheral blood mononuclear cells (pbmc) and measured the plasma level of four neurohormones. 2. in 36 subjects, decreasing t lymphocyte response to con a covaried with age. percent of lymphocytes labeled as t8 lymphocytes tended to decrease and t4/t8 ratio tended to increase with increasing age. 3. hamilton and beck scores were significantly different between the two sex and ...	1988	3265523
[epidemiology and means of transmission of hiv infection].	epidemiological features of hiv infection and the aids pandemic are described in this review. the number of aids cases will continue to rise for several years, despite enormous efforts to slow the spread of hiv via sexual and parenteral exposure, since virtually all adult patients who will develop aids within the next 3-5 years are already infected. careful examination of aids patients without identified risk factors and results obtained from anonymous testing sites for hiv infection have failed ...	1988	3266281
effect of recent antibacterial agents against bacteria causing diarrhoea.	the susceptibility of 424 bacterial isolates causing diarrhoea were tested by agar dilution technique on mueller-hinton agar against amoxicillin, ampicillin, ceftriaxone, chloramphenicol, co-trimoxazole, ciprofloxacin, doxycycline, norfloxacin and ofloxacin. the bacterial species included were aeromonas hydrophila, edwardsiella tarda, pleisomonas shigelloides, salmonella spp., shigella spp., vibrio cholerae and vibrio parahaemolyticus. the most active compounds were the fluorinated 4-quinolones ...	1988	3266900
[relationship between the pathogenicity and the toxigenicity of vibrio cholerae 01 and vibrio cholerae not 01].		1988	3268051
[role of neuraminidase in the pathogenicity of vibrio cholera 01 and vibrio cholerae not 01].		1988	3268055
effect of resin components on the growth of streptococcus mutans.		1988	3269405
light and electron microscopic studies of experimentally-induced pathologic changes in the rat periodontal tissue.		1988	3271013
light and electron microscopic studies of experimentally-induced pathologic changes in the rat periodontal tissue.		1988	3271013
the annual cycle of zooplankton-associated vibrio cholerae and related vibrios in albufera lake and its coastal surrounding waters (valencia, spain).	most probable numbers of zooplankton-associated vibrio spp. and v. cholerae were determined in albufera lake, spain, and in its coastal receiving waters throughout a year. highest counts of 10(5) bacterial cells/g of plankton were associated to high temperatures and were also related to the kind of water. all isolates were non-01 serovars, and most belonged to heiberg groups i and ii.	1988	3271065
volunteer studies of deletion mutants of vibrio cholerae o1 prepared by recombinant techniques.	vibrio cholerae o1 a-b- vaccine strain jbk 70 and a-b+ cvd 101 prepared by recombinant dna techniques from pathogenic ei tor inaba n16961 and classical ogawa 395, respectively, were fed to 38 volunteers in single doses of 10(4) to 10(10). although severe diarrhea did not occur in any vaccine, more than one-half developed mild diarrhea. these attenuated strains colonized well and elicited prominent vibriocidal and antitoxic (cvd 101) antibody responses. recipients of a single dose of jbk 70 were ...	1988	3335402
studies on compound i formation of the lignin peroxidase from phanerochaete chrysosporium.	ligninase, isolated from the wood-destroying fungus phanerochaete chrysosporium, catalyzes the oxidation of lignin and lignin-related compounds. ligninase reacts with h2o2 to form the classical peroxidase intermediates compounds i and ii. we have determined the activation energy of ligninase compound i formation to be 5.9 kcal/mol. the effect of ph and ionic strength on the rate of ligninase compound i formation was studied. in contrast to all other peroxidases, no ph effect was observed. this i ...	1988	3335539
five-year immunologic memory in swedish volunteers after oral cholera vaccination.		1988	3335813
effects of cyclosporin a and predegeneration on survival and regeneration of peripheral nerve allografts in rabbits.	axonal regeneration across 64 median nerve grafts in 34 rabbits was studied histologically to determine the optimal conditions for nerve allografting. axonal regeneration across allografts in cyclosporin a-treated animals was satisfactory, but it occurred more slowly and with more inflammation and fibrosis than in autografts. without immunosuppression, fresh allografts were rejected. however, in immunocompetent hosts, allografts rendered less immunogenic by predegeneration were not rejected, and ...	1988	3336858
[protective effect of lithium oxybutyrate on the ischemic myocardium].	in the experiments on different animal species (mice, cats, dogs) lithium hydroxybutyrate has been shown to have antihypoxic and anti-ischemic effects. lithium hydroxybutyrate improved the functional state of the ischemic myocardium, stimulated the accumulation of macroergic phosphates (atp) in the heart, protected the ischemic myocardium and delayed the progression of the reversible ischemic damage into the irreversible one. the improvement of the collateral coronary circulation plays an import ...	1988	3337880
iron metabolism in the erythrophagocytosing kupffer cell.	like the peritoneal macrophage, the isolated kupffer cell is capable of processing and releasing iron acquired by phagocytosis of immunosensitized homologous red blood cells. when erythrophagocytosis is restrained to levels which do not affect cell viability, or less than 1.5 red cells/macrophage (phagocytic index of 150%), over 40% of iron acquired from red cells is released within 24 hr. more active erythrophagocytosis results in greater release of iron but progressive deterioration in cell vi ...	1988	3338718
the cpg dinucleotide and human genetic disease.	reports of single base-pair mutations within gene coding regions causing human genetic disease were collated. thirty-five per cent of mutations were found to have occurred within cpg dinucleotides. over 90% of these mutations were c----t or g----a transitions, which thus occur within coding regions at a frequency 42-fold higher than that predicted from random mutations. these findings are consistent with methylation-induced deamination of 5-methyl cytosine and suggest that methylation of dna wit ...	1988	3338800
induction of secondary ige antibody response in rats immunized with x-irradiated metacercariae of paragonimus ohirai.	rats reinfected with paragonimus ohirai (p.o.) elicited little secondary ige response to adult p.o. antigen. in contrast, rats immunized with x-irradiated (2-10 krad) metacercariae elicited not only a marked primary ige response comparable to that in normally infected rats, but also a marked secondary ige response after challenge infection. rats immunized with 2 krad x-irradiated metacercariae yielded a higher secondary ige response than with 5 or 10 krad irradiated ones, and elicited a secondar ...	1988	3338861
antigens involved in resistance to mucosal association by vibrio cholerae.	resistance to growth of vibrio cholerae at the mucosa of blind intestinal loops developed in rats after intestinal or parenteral exposure to live organisms or other antigenic materials. simultaneous serological studies suggested that neither serum vibriocidal activity nor intestinal mucus antibodies are likely to provide a direct test of antibacterial immune status. challenge of rats 4 weeks after one dose of antigen may reveal a form of immunity that is not related to antibodies in the intestin ...	1988	3339633
induction and survival of micronuclei in rat spermatids. comparison of two meiotic micronucleus techniques using cyclophosphamide.	the induction and survival of micronuclei (mn) in rat spermatids was studied by two different methods, the dissection method (dm) and the suspension method (sm). it was observed that mn are induced by cyclophosphamide in the s phase of meiosis, in preleptotene spermatocytes, and in an earlier cell stage, the type b spermatogonia. both techniques showed that mn survive in spermatids at least 5 days. advantages of the dm include the use of a dna-specific fluorochrome for staining of mn, higher mn ...	1988	3340092
expression of heme oxygenase gene in rat and human liver.	developmental changes of microsomal heme oxygenase were studied. in human fetal liver, the enzyme activity was 8 times higher than that detected in the adult liver. on the other hand, adult livers contained 4 times more cytochrome p450 than fetal livers. elevated heme oxygenase activity in fetal liver was not due to modulators present in the microsomes. similar to human, rat fetal liver also contained high enzyme activity which appeared to be regulated at the transcriptional level. hybridization ...	1988	3342043
anterior perineal dislocation of the hip with fracture of the femoral head. a case report.	traumatic anterior perineal hip dislocation with an associated fracture of the femoral head is a rare entity. a 26-year-old man injured in a motorcycle accident was treated by closed reduction of the dislocation within three hours after admission. however, several reports of patients with anterior hip dislocation with associated femoral head fractures were treated nonoperatively and had unfavorable results when treatment failed to achieve anatomical position of the fragments. consequently, this ...	1988	3342566
selective decrease in low-mr hmg proteins hmg i and hmg y during differentiation of mouse teratocarcinoma cells.	we have studied the presence of high-mobility-group (hmg) chromatin proteins in undifferentiated f9 mouse teratocarcinoma cells and f9 cells, which were induced to differentiate by treatment with retinoic acid and dibutyryl-camp for 5 days. acetic acid/urea-polyacrylamide gel electrophoresis and reversed-phase hplc revealed that the induced f9 cells contained 77 and 62% less hmg i and hmg y, respectively, than their untreated counterparts. the relative amounts of two other low-mr hmg proteins hm ...	1988	3342876
lactic acidosis and recovery of neuronal function following cerebral hypoxia in vitro.	the rat hippocampal slice preparation was used to study the combined effects of hypoxia and lactic acidosis on neuronal function. control slices were exposed to a standard hypoxic insult while being perfused with normal artificial cerebrospinal fluid (acsf). experimental slices were perfused with acsf containing 1.0, 2.0, 10.0 or 20.0 mm lactic acid, 30 min before and during the same standard hypoxic insult. following at 30-min recovery period the ability of these slices to respond to orthodromi ...	1988	3345436
the transducer characteristic of hair cells in the human ear: a possible objective measure.	we describe here a new kind of non-linear interaction between the responses to two simultaneously presented am tones. the interaction occurs before the level of binaural convergence, and can be explained in terms of the shape of the inner ear hair cell transducer function.	1988	3345444
c-h-ras and c-k-ras gene hypomethylation in the livers and hepatomas of rats fed methyl-deficient, amino acid-defined diets.	the extent of methylation of the c-h-ras and c-k-ras oncogenes was compared in neoplastic and preneoplastic livers of rats fed one of several methyl-deficient, amino acid-defined diets for 18 months, with or without a preceding initiating dose of diethylnitrosamine (den). the restriction endonucleases mspi, hpaii, hhai, paer71 and xhoi were used for studying the extent and pattern of dna methylation. the results indicated that both c-h-ras and c-k-ras oncogenes were hypomethylated in all dna sam ...	1988	3345576
fc receptor-mediated phagocytosis occurs in macrophages at exceedingly low cytosolic ca2+ levels.	cytosolic free ca2+ ([ca2+]i) homeostasis was investigated in mouse peritoneal macrophages and in the macrophage-like cell line j774. [ca2+]i measurements were performed in both cells in suspension and cells in monolayers loaded with either quin2 or fura-2. resting [ca2+]i was 110-140 and 85-120 nm for cell suspensions and monolayers, respectively. there were no significant differences in [ca2+]i between the two macrophage populations whether quin2 or fura-2 were used as ca2+ indicators. additio ...	1988	3346321
peptides bound to proteosomes via hydrophobic feet become highly immunogenic without adjuvants.	addition of either a lauroyl or a pentapeptide (fllav) hydrophobic foot to the nh2 terminus of a small, synthetic peptide allowed the peptide to hydrophobically complex to meningococcal outer membrane protein proteosomes by simple dialysis. both conventional and lps-hyporesponsive mice immunized with these complexes without any adjuvants developed high-titered and persistent anti-peptide igg. since proteosomes have been safely given to many people and since important antigenic determinants are g ...	1988	3346624
estrogen- and antiestrogen-induced ornithine decarboxylase activity and uterine growth in the rat.	the estrogen antagonists tamoxifen and monohydroxytamoxifen are also classified as partial estrogen agonists. in infantile rats, estradiol induced a single peak of uterine odc activity at 6h following injection regardless of the extent of induction by various estradiol doses. by contrast, the timing of the odc activity peak induced by tamoxifen and monohydroxytamoxifen was highly dependent upon the dosing conditions and was delayed to 18 h at lower tamoxifen doses. in immature rats, tamoxifen an ...	1988	3347055
measurement of ultrasonic nonlinear parameter in excised fat tissues.	this paper reports sound speed co, adiabatic acoustic nonlinear parameter b/a, and isothermal nonlinear parameter (b/a)' of 33 samples of excised human and animal fats and some simple mixtures, e.g., skim milk and powdered milk. tissues were obtained from different parts of the body and the measurements were made in the temperature range of 20 degrees c to 37 degrees c. all the fats were highly nonlinear. although there was a considerable overlap in the values of acoustic nonlinearity of differe ...	1988	3347965
stimulation of rabbit lacrimal gland secretion with biologically active peptides.	to determine whether biologically active peptides can stimulate lacrimal gland secretion, we measured fluid and protein secretion from the cannulated lacrimal gland excretory duct of anesthetized rabbits after arterial injection of various peptides. vasoactive intestinal peptide (vip, 0.003-3 nmol) and porcine histidine isoleucine-containing peptide (phi, 0.01-3 nmol) stimulated fluid and protein secretion in a dose-dependent manner. cholecystokinin octapeptide (cck-8, 0.01-3 nmol) stimulated fl ...	1988	3348396
age-dependent changes in in vivo ethanol metabolism and in the activity of hepatic enzymes involved in ethanol oxidation and microsomal functions.	the study of the influence of the age of the animals (13 to 53 weeks) on the rate of ethanol metabolism in vivo and the total activity of liver alcohol dehydrogenase and microsomal ethanol oxidizing system showed a progressive decline with age. these effects were observed concomitantly with a diminution in the content of cytochrome p-450 and microsomal functions related to oxidative and free-radical mediated reactions, namely, nadph oxidase activity, nadph-dependent oxygen uptake and nadph-or t- ...	1988	3349570
coverage of a carpal tunnel wound dehiscence with the abductor digiti minimi muscle flap.	a 41-year-old man who was taking 60 mg of prednisone a day for dermatomyositis developed a wound dehiscence following a carpal tunnel release and flexor tenosynovectomy. coverage of the exposed carpal contents was provided by using the abductor digiti minimi as a local transposition flap.	1988	3351226
ultrastructural aspects of in vitro fertilization in sheep.	fertilization events of the sheep oocyte have been analyzed by electron microscopy. in the fertilization cone, near the decondensing sperm nucleus, several areas composed of electron-dense filamentous material (fa) were observed. moreover aster-like structures were present in the vicinity of the sperm neck and proximal midpiece. during swelling of the pronuclei, redistribution of cytoplasmic organelles occurred near the male pronucleus in a region facing the center of the egg. in this region, co ...	1988	3351351
improvement of growth and food utilization by human recombinant growth hormone in uremia.	we compared growth rate, food conversion ratio and morphology of the growth zone in female sprague-dawley rats with subtotal nephrectomy or sham operation. both groups were either given vehicle or 1.4 iu/day recombinant human growth hormone (gh) by s.c. osmotic minipump, or 2.5 iu twice daily intraperitoneally for 14 or 20 days, respectively. compared to uremic rats infused with vehicle, infusion of gh significantly (p less than 0.01) improved growth; that is, it increased gain of weight (delta ...	1988	3352160
establishment and characterization of a human urinary bladder carcinoma cell line (tsgh-8301).	a cell line derived from a well-differentiated human transitional cell carcinoma of the urinary bladder, designated tsgh-8301, was established in vitro. the cultured epithelioid cells exhibited monolayer growth and loss of contact inhibition. the tumorigenicity of tsgh-8301 had been shown by growth in soft agar and tumor induction in athymic nude mice. a reverse ratio of lactate dehydrogenase (lhd) isoenzyme in the cell line and nude mouse-grown tumors was seen predominantly with ldh-v. chromoso ...	1988	3352272
anti-inflammatory activity of oleoresin from brazilian copaifera.	the oleoresin from brazilian copaifera species yielded copalic acid and sesquiterpenes and showed marked anti-inflammatory activity using various experimental models in rats. the oleoresin significantly inhibited carrageenin-induced pedal edema following oral doses from 0.70 to 2.69 ml/kg, but was somewhat less effective than 50 mg/kg calcium phenylbutazone. repeated administration of the oleoresin at a dose of 1.26 ml/kg for a 6-day period reduced granuloma formation with a response comparable ...	1988	3352280
effect of teucrium polium boiled leaf extract on intestinal motility and blood pressure.		1988	3352281
effects of mezerein and diglycerides on ornithine decarboxylase activity in mouse mammary gland explants.	one of the most rapid actions of prolactin in mouse mammary gland explants is the stimulation of ornithine decarboxylase (odc) activity. several protein kinase c activators including mezerein, dicaprin, diolein, and 1-oleoyl-2-acetyl-rac-glycerol were found to stimulate odc activity as does prolactin. both mezerein and the diglycerides produced nonadditive responses when tested with maximum stimulatory concentrations of prolactin. the results of these studies therefore provide further evidence t ...	1988	3353391
regional blood flows by the microsphere method: reproducibility in portal hypertensive rats and influence of a portal vein catheter.	to determine the reproducibility of splanchnic blood flow measurements by the microsphere method in rats with portal hypertension and the effects of laparotomy with portal vein cannulation, eight groups of 10 rats were studied. cardiac output and regional blood flows were measured twice, 10 min apart, in pentobarbital anesthetized or awake, sham-operated or portal vein-ligated rats, with or without portal cannulation. variability between the two successive measurements was not affected by portal ...	1988	3353395
time-space clustering of vibrio cholerae 01 in matlab, bangladesh, 1970-1982.	growing evidence for the existence of an aquatic reservoir of vibrio cholerae has led some observers to postulate the existence of two distinct modes of disease transmission: primary and secondary. in primary transmission vibrios pass from the aquatic reservoir to humans via edible aquatic flora or fauna, or drinking water. secondary transmission consists of faecal-oral transmission from person-to-person and may spawn epidemics. cholera outbreaks are particularly well documented for the matlab a ...	1988	3353754
bioalkylation and biooxidation of anthracene, in vitro and in vivo.	anthracene undergoes biomethylation in rat liver cytosol preparations in vitro and in rat subcutaneous tissue, in vivo. the in vitro reaction is dependent on cytosol preparations fortified by the addition of s-adenosyl-l-methionine. the products of the reaction are the meso-anthracenic or l-region derivatives 9-methylanthracene and 9,10-dimethylanthracene. the latter compound may be the simplest polynuclear aromatic hydrocarbon carcinogen known. these reactive methylated metabolites are readily ...	1988	3355564
epidemic cholera in mali: high mortality and multiple routes of transmission in a famine area.	during the 1984 cholera epidemic in mali, 1793 cases and 406 deaths were reported, a death-to-case ratio of 23%. in four affected villages, the mean clinical attack rate was 1.5 and 29% of affected persons died. in 66% of cases the illness began more than 48 h after the village outbreak began, when supplies from outside the village were potentially available. deaths occurred because patients failed to seek care or received only limited rehydration therapy when they did. case-control studies iden ...	1988	3356224
[dynamics of unit activity of the gigantocellular tegmental field in the sleep-wakefulness cycle of rats].	computer analysis and statistical processing of the unit activity showed that the majority of neurons (65.2%) in the gigantocellular tegmental field were discharging with high frequency during active wakefulness and paradoxical sleep as compared to slow-wave sleep in unrestrained rats. 11.6% of the neurons revealed an inverse pattern of activity: they discharged more intensively during slow-wave sleep, their firing rate decreasing during active wakefulness and paradoxical sleep. some neurons (23 ...	1988	3356265
sexually transmitted diseases, antibodies to human immunodeficiency virus, and subsequent development of acquired immunodeficiency syndrome. visitors of homosexual sauna clubs in copenhagen: 1982-1983.	sera from 260 men from denmark and elsewhere attending two copenhagen sauna clubs for homosexual men during nine months of 1982-1983 were investigated for markers for syphilis, hepatitis a and b, and human immunodeficiency virus (hiv). five per cent (12 men) had active syphilis, and another 35% (92) had a history of and/or serologic markers for syphilis. ninety-four men (36%) were positive for antibodies to hepatitis a virus, ten (4%) were positive for hepatitis b surface antigen (hbsag), and 15 ...	1988	3358236
[determination of the partial specific volume of cytochrome p450 lm2].	the partial specific volume (v) of highly purified membrane protein cytochrome p450 lm2 monooxygenase from rabbit liver endoplasmatic reticulum has been estimated by various independent methods. the values of v obtained through our experiments are practically equal to the value calculated from the amino acid composition of the protein (0.75 cm3/g).	1988	3358967
regional membrane heterogeneity in premyelinated cns axons: factors influencing the binding of sterol-specific probes.	binding of the sterol-specific probe filipin to developing optic nerve axonal membrane is spatially heterogeneous prior to association of glial cells with the axons. experiments were performed using different sterol binding probes (filipin, tomatin, and saponin), at different temperatures (4 degrees c, 23 degrees c, and 37 degrees c), after incubation in different ionic conditions (10 mm ca2+, 10 mm egta, and 20 mm mg2+), to examine factors that may be responsible for this membrane heterogeneity ...	1988	3359268
(+/-)-beta-parachlorophenylglutamate selectively enhances the depolarizing response to l-homocysteic acid in neocortical neurons of the rat: evidence for a specific uptake system.	the effect of (+/-)-beta-parachlorophenylglutamate (cp) on depolarizations induced by iontophoretically applied l-glutamate, l-aspartate, l-homocysteate (l-hca) and d-hca was investigated in neurons of the rat neocortex in vitro. cp, a reported blocker of amino acid uptake, strongly enhanced l-hca responses whereas responses to the other amino acids remained little affected. this action was observed irrespective of whether cp was administered iontophoretically or pneumatically from micropipettes ...	1988	3359277
effects of short-term prenatal alcohol exposure on neuronal membrane order in rats.	long-evans rat dams were treated with ethanol (4 g/kg, twice daily) by gavage on gestational days 10-14. this dosage schedule has been shown to produce significant behavioral and ponderal teratogenicity. pair-fed dams were gavaged with isocaloric amounts of sucrose. all offspring were reared by untreated, surrogate dams. pups were sacrificed on days 3 and 28, and whole brain neuronal plasma membranes were prepared for analysis by a fluorescence polarization technique using 1,6-diphenyl-1,3,5-hex ...	1988	3359307
does sphingomyelin inhibit the erythrocyte anion transport system?	the anion transport protein of the human erythrocyte membrane, band 3, was incorporated into unilamellar sphingomyelin vesicles. the vesicles showed a rapid sulfate efflux which could be inhibited by specific inhibitors of the erythrocyte anion transport system. all band 3 molecules contributing to the inhibitor-sensitive flux component were arranged 'right-side-out'. the turnover number of the transport protein for sulfate transport was virtually identical to that in phosphatidylcholine bilayer ...	1988	3360128
purification and characterization of a family of high molecular weight surface-array proteins from campylobacter fetus.	a variety of gram-negative and gram-positive bacteria possess crystalline surface layers, although little is known of their function. we previously have shown that the high molecular weight surface-array proteins of campylobacter fetus are important in both the pathogenicity and antigenicity of this organism. for biochemical and immunological characterization, we purified high molecular weight (100,000, 127,000, 149,000) surface-array proteins from three c. fetus strains using sequential gel fil ...	1988	3360785
effects of chronic phthalate exposure on the kidney.	several investigators have reported the finding of polycystic kidney disease (pkd) at autopsy in patients who had undergone long-term hemodialysis for renal failure due to causes other than pkd. we initiated studies to determine whether the drugs or chemicals to which patients on dialysis are exposed could be responsible for these cystic changes. adult rats were tube fed chemical residues from an artificial kidney or phthalate esters [di(2-ethylhexyl) phthalate, dehp], which are a main component ...	1988	3361614
artificial insemination with fresh semen in beagle bitches.		1988	3361726
measurement of small-intestinal motor activity in the preterm infant.	the development of motor activity within the small intestine of preterm human infants is very poorly understood. using a miniaturized multilumen continuous perfusion manometric technique the motor activity of the small intestine was followed longitudinally in a group of preterm infants. the system performed well at very low perfusion rates and clearly showed increases in both the magnitude and organization of motor activity with increasing gestational age.	1988	3361871
tissue oxygen uptake from the atmosphere by a new, noninvasive polarographic technique with application to corneal metabolism.	a standard clark electrode has been modified to continuously monitor the po2 in a thin, disk shaped reservoir membrane mounted on the electrode membrane surface. in vitro tests were conducted to determine the proportionality constant between the rate of change of reservoir po2 and the flux of o2 out of the reservoir. the device was then used to determine the corneal o2 uptake on proptosed eyes of previously sacrificed rabbits. our average measured uptake at 20 degrees c, 1.7 microliter/cm2.hr, a ...	1988	3364251
mate recognition by urine odors in the mongolian gerbil (meriones unguiculatus).	we examined the responses of male and female gerbils housed in opposite- or same-sex pairs to the urine odors from their cage-mate and those of an unfamiliar gerbil of the same sex. gerbils housed in mated pairs spent more time investigating the odor of their partner. they also emitted more modulated ultrasonic vocalizations in the presence of their partner's odor than in the presence of the unfamiliar odor. gerbils housed in same-sex pairs did not respond differentially to the odor of their cag ...	1988	3365186
n-acetylcysteine inhibits human neutrophil and monocyte chemotaxis and oxidative metabolism.	the effect of n-acetylcysteine (nac) on human neutrophil and monocyte cell viability, chemotaxis, oxygen consumption and chemiluminescence was studied. it was found that nac at concentrations higher than 3 x 10(-2) m resulted in neutrophil and monocyte cytotoxicity. the studies on the effect of nac on neutrophil and monocyte chemotaxis showed that nac inhibited chemotaxis of both cell types in a concentration dependent manner. nac at 3 x 10(-2) m inhibited chemotaxis of both cell types by about ...	1988	3366508
a comparison of the effects of measured, predicted, estimated and constant residual volumes on the body density of female athletes.	the body density (bd), and hence the relative body fat (% bf) was measured for 182 female athletes. the residual volume (rv) was determined both before and after the underwater weighing by a multiple breath helium dilution technique with the subject immersed to neck level. the absolute mean difference (lxdl) and see between the two rv trials were 63 and 75 ml, respectively. these increased to values ranging 144-685 and 187-252 ml, respectively, when the mean of the two rv trials for each subject ...	1988	3366515
antimicrobial susceptibility testing of streptococcus pneumoniae: quality assessment results.	six strains of streptococcus pneumoniae were distributed to 405 united kingdom laboratories who were asked to test the susceptibility of the strains to penicillin, tetracycline, chloramphenicol and erythromycin and to provide details of methodology to test the standards of susceptibility testing. high error rates were seen only in failure to detect moderate resistance to penicillin (12%) and resistance to chloramphenicol (16%). increased error rates were associated with several methods or practi ...	1988	3366921
[topical differences in the electric changes in the ischemic area in the in situ heart in hogs].		1988	3368613
[peculiarities of nicotinic acid formation in coronary heart disease with special reference to heart arrhythmias].	the present work is divided into two parts: clinical observations and experimental investigations. the endogenic formation of nicotinic acid was decreased in all patients with ischaemic heart disease, and we have found an increased accumulation of kynurenine in blood serum after tryptophan loading in many cases - a clear indication of pyridoxal-5-phosphate (p-5-p) deficiency in the organism. the results of experimental investigations showed that l-kynurenine sulphate initiates cardiac dysrhythmi ...	1988	3369217
[effect of prolactin on the enzymatic activity of thyroid follicular cells during stress due to surgery and subsequent subcutaneous inflammation].	in order to reveal prolactin (prl) effect on the enzymatic activity of the thyroid follicular cells at a subcutaneous inflammation, 2 series of experiments have been performed on 100 non-inbred male rats with body mass of 150-170 g. in order to produce the inflammation, under ether narcosis celloidin globules are introduced subcutaneously in the abdominal wall. the test animals are given 0.125 mg of "lactin" (the soviet preparation of bovine prl) in 0.5 ml of isotonic solution once every day, be ...	1988	3369954
neuronal activity recorded extracellularly from in situ canine mediastinal ganglia.	spontaneous activity of 226 neurons was recorded from in situ mediastinal ganglia in 10 dogs. forty-two percent of these were active during specific phases of the cardiac cycle, primarily during systole. cardiovascular-related activity occurred when systolic pressure was between approximately 70 and 185 mmhg (1 mmhg = 133.3 pa) whether the pressure was altered by positive inotropic pharmacological agents or cross clamping of the aorta. twenty percent of the identified neurons displayed respirato ...	1988	3370543
time course of adaptation to hypoxia in newborn rats.	in newborn rats after a few minutes of hypoxia, ventilation is similar to the normoxic value. nevertheless, after a few days in hypoxia, newborn rats have a sustained hyperventilation. in this study we examined the time course of the newborn rat's adaptation to hypoxia. measurements of body size, hematocrit, lung and heart mass, and breathing pattern have been performed on newborn rats exposed to hypoxia (10% o2) for different time intervals from 4 to 60 h (hypoxic, h), and on same-age rats grow ...	1988	3370546
haemophilus b conjugate vaccine.		1988	3370570
freeze-fracture and cytochemistry study of the interaction between leishmania mexicana amazonensis and macrophages.	the process of interaction between macrophages and promastigote and amastigote forms of leishmania mexicana amazonensis was analyzed using freeze fracture and cytochemistry. the promastigotes inside endocytic vacuoles of macrophages presented an altered distribution of intramembranous particles and a wavy aspect of the plasma membrane. however, amastigotes did not show such alterations. the membrane alterations are probably caused by intracellular cell lysis of the promastigotes by the macrophag ...	1988	3370624
inhibition by dietary ethanol of experimental colonic carcinogenesis induced by high-dose azoxymethane in f344 rats.	epidemiological studies have shown an association between consumption of alcoholic beverages and increased occurrence of large bowel carcinoma, but studies in experimental models of colonic carcinogenesis have produced conflicting results. we assessed the effects of chronic dietary ethanol consumption during the preinduction and induction phase (period of acclimatization and carcinogen administration) in a high-dose azoxymethane-treated rat model (14 mg/kg/wk for 10 wk). ten-wk-old male fischer ...	1988	3370634
whole blood storage solution for erythrocyte sodium and sodium-lithium countertransport rate determination.	a solution consisting of heparinized and buffered isosmolar magnesium chloride is described in which whole blood may be stored for preservation of erythrocyte sodium (na) and membrane sodium-lithium countertransport (na-li ct). correlation (r) of fresh versus 24-h stored erythrocytes for na was 0.990 (y = 0.30 + 0.956x) and for na-li ct was 0.995 (y = -0.014 + 1.022x). na-li ct rate was preserved in the storage solution for up to five days and erythrocyte na concentration for at least 24 h. the ...	1988	3370838
response of the jejunal mucosa of dogs with aerobic and anaerobic bacterial overgrowth to antibiotic therapy.	dogs with naturally occurring aerobic or anaerobic bacterial overgrowth have been examined before and after antibiotic therapy in order to assess reversibility of damage to the jejunal mucosa. histological changes in peroral jejunal biopsies were relatively minor before and after treatment, but sucrose density gradient centrifugation revealed specific biochemical abnormalities that responded to antibiotic therapy. aerobic overgrowth was initially associated with a marked loss of the main brush b ...	1988	3371716
a serogroup of non-o1 vibrio cholerae possessing the inaba antigen of vibrio cholerae o1.	a serogroup of non-o1 vibrio cholerae, tentatively named hakata, possessing the c (inaba) factor but not the b (ogawa) and a factors of v. cholerae o1 is described. strains of this serogroup were isolated from river and estuarine waters and from frozen shrimps.	1988	3372399
prolonged increased responsiveness of canine peripheral airways after exposure to o3.	because it is relatively insoluble, the oxidant gas o3 may penetrate to small peripheral airways when it is inhaled. increased responsiveness in large airways after o3 breathing has been associated with the presence of inflammatory cells. to determine whether o3 produces prolonged hyperresponsiveness of small airways associated with the presence of inflammatory cells, we exposed the peripheral lungs of anesthetized dogs to 1.0 ppm o3 for 2 h using a wedged bronchoscope technique. a contralateral ...	1988	3372418
g-actin structure revealed by chymotryptic digestion.	the chymotryptic digestion of g-actin in the presence of calcium produces not only a c-terminal 33 kda "core," spanning from residue 68 to the c-terminus, but also an n-terminal cys-10-containing fragment (10 kda fragment), spanning from the n-terminus to the 44th residue. the minimum calcium concentration required for producing just these two structures is 10(-7.5) m. in a ca medium, the 10 kda fragment remains attached to the 33 kda core, and the 10 kda fragment detaches when the divalent cati ...	1988	3372494
cell surface sialylation and tumor metastasis. metastatic potential of b16 melanoma variants correlates with their relative numbers of specific penultimate oligosaccharide structures.	numerous investigations suggest that cell surface glycoconjugates, and in particular sialic acids, are directly involved in determining the metastatic phenotype. to further evaluate this hypothesis, we have used a variety of techniques to probe the cell surfaces of several metastatic variants of the murine b16 melanoma that were selected for experimental lung-colonizing ability (fidler, i. (1973) nature 242, 148-149) or for their ability to spontaneously metastasize from the site of a subcutaneo ...	1988	3372501
high-performance liquid chromatographic determination of nitrazepam in plasma and its application to pharmacokinetic studies in the rat.		1988	3372657
a method of obtaining, processing, and analyzing human intestinal secretions for antibody content.	human intestinal secretions can be readily obtained using a commercially available intestinal lavage solution. although such secretions contained abundant protease activity, significant loss of immunoglobulins was prevented by the addition of a mixture of protease inhibitors. the total content of iga, igm, and igg antibody in secretions was measured using sandwich elisa. in the secretions of ten normal volunteers iga was most abundant (197 micrograms/ml +/- 103 sd) followed by igm (12.5 microgra ...	1988	3373004
[molecular weight and restriction mapping of the dna of cholera phages].	molecular masses of cholera bacteriophages 493, 7226 and eltor ii were defined by electron microscopic technique. dna of these bacteriophages was digested by the restriction endonucleases psti, bgli, mlui and sali. the number and molecular masses of the obtained restricts were identified. the physical map of bacteriophage 493 was constructed using three restriction endonucleases. the obtained data can be used for classification and molecular biology research of cholera bacteriophages.	1988	3374504
[evaluation of the results of performance tests for stallions and the swiss saddle horse shows].		1988	3375797
[the shaping of the behavioral act: the problem of intrasystemic heterochrony].	according to p.k. anokhin's conception, the functional system of every behavioural act includes one and the same sequence of basic mechanisms, the main of them are afferent synthesis and making decision, realization of action, achievement of the result and evaluation of its parameters. the present work deals with the question about the extent of coordination in the development of these systemic mechanisms in the process of new behavioural act formation.	1988	3376566
epithelial distribution in the human fetal larynx.	scanning and transmission electron microscopy and light microscopy were used to study the distribution of squamous epithelium and ciliated respiratory type epithelium in ten midtrimester human fetal larynges. particular attention was paid to the subglottic region, where previous studies have reported areas of squamous metaplasia in neonates, infants, and nonsmoking adults. both types of epithelium were found in different proportions in the supraglottic, glottic, and subglottic regions. there was ...	1988	3377398
cholera toxin neutralization: a comparison of purified serum igg and biliary secretory iga antibodies.	rats were immunized three times with cholera toxin via the intraintestinal or intravenous route, and their respective biliary secretory iga (siga) or serum igg antibodies were affinity-purified on a cholera toxin immunoabsorbent. on a molar basis, the siga antibodies were roughly seven-fold more efficient than igg antibodies in neutralizing cholera toxin in the ligated intestinal loop assay. various explanations for this difference in neutralizing capacity are proposed.	1988	3378829
[the effect of vibrio-cholerae neuraminidase (vcn) on the nk activity of peripheral blood lymphocytes in patients with cancer].		1988	3379327
structural and functional characterization of mouse u7 small nuclear rna active in 3' processing of histone pre-mrna.	oligonucleotides derived from the spacer element of the histone rna 3' processing signal were used to characterize mouse u7 small nuclear rna (snrna), i.e., the snrna component active in 3' processing of histone pre-mrna. under rnase h conditions, such oligonucleotides inhibited the processing reaction, indicating the formation of a dna-rna hybrid with a functional ribonucleoprotein component. moreover, these oligonucleotides hybridized to a single nuclear rna species of approximately 65 nucleot ...	1988	3380087
bacteremia in a veteran's administration extended care facility.		1988	3380833
the defect of lymphocyte locomotion in chronic lymphocytic leukaemia: studies of polarization and growth-dependent locomotion.	this paper reports a study of the locomotor behaviour of the lymphocytes from 17 patients with chronic lymphocytic leukaemia (cll). the cells were studied both immediately after separation from blood and after culture for 24 to 48 h with a range of growth activators. cells direct from blood were tested for polarization in fetal calf serum (fcs 20%), phorbol myristate acetate (pma 10(-7)m) and colchicine (10(-5)m). the polarization of lymphocytes from cll patients with high white cell (wbc) count ...	1988	3383451
age-dependent variation for inducibility of metallothionein genes in mouse liver by cadmium.	cadmium is a toxic metal that induces the expression of metallothionein genes in many tissues and that binds avidly to metallothionein, a soluble transition metal binding protein. the present study examined the temporal pattern and magnitude of accumulation of metallothionein mrna in liver of c57bl/6j mice of various ages treated with cadmium. in adult female mice, accumulation was dependent on the dosage level of cadmium and related to the concentration of this metal in liver. the accumulation ...	1988	3383454
protein kinase c in the developing rat liver, heart and brain.	ontogenic changes of protein kinase c in the rat liver, heart and cerebrum were examined from 17-day gestation until adult. cerebral protein kinase c activity was 19 times less at 17-day fetal as compared to 15-day postnatum or older rats. the enzyme activity in the heart was generally higher in the neonatal period than the adult, but was not correlated with the previously reported alpha 1-adrenergic and cholinergic receptor ontogeny. likewise in the liver, the enzyme activity was not correlated ...	1988	3383720
crystallization of cytochrome p-450scc from bovine adrenocortical mitochondria.	cytochrome p-450scc (p-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (plp) by microdialysis against distilled water, reddish and planar crystals appeared. the crystals of native p-450scc were also obtained by the same procedure. we identified the crystals as the p-450scc-plp complex or native p-450scc by absor ...	1988	3384090
changes of cortical and caudatal unit activity accompanying operant slowing of the extension phase of reaching in rats.	activity of neurons in the motor cortex (n = 36) and caudate nucleus (n = 72) was studied in 7 rats trained to slow down reaching into a narrow horizontal tube equipped with an axially moving piston. overtrained rats succeeded in obtaining reward by reducing extension velocity of 30 to 60% reaches below 50% of the normal value. the difficulty of the task was reflected in the amplitude of perireach histograms which showed peaks and troughs corresponding to the main phases of the movement. perirea ...	1988	3384566
intracellular development of choleraphage phi 149 under permissive and nonpermissive conditions: an electron microscopic study.	intracellular development of choleraphage phi 149 following infection of vibrio cholerae and vibrio eltor cells under different conditions was examined by thin-section electron microscopy. degradation of the host dna following infection and formation of mature phage particles inside the infected cells were demonstrated. the concatemeric dna intermediate formed during intracellular replication of phi 149 dna in permissive hosts was resolved. in confirmation of biochemical evidence, no concatemeri ...	1988	3384631
enterotoxigenicity of chicken isolates of campylobacter jejuni in ligated ileal loops of rats.	ligated ileal loops in rats of the charles-foster strain, weighing 200-250 g and aged 7-8 months, provided a sensitive and reproducible means of testing the enterotoxigenicity of campylobacter jejuni. all of 16 chicken isolates caused fluid accumulation comparable to that produced by toxigenic vibrio cholerae o1, strain 569b. however, 11 of the isolates required one to three consecutive passages through the rat gut before doing so. the amount of fluid produced increased after each passage. of th ...	1988	3385766
ct-like enterotoxin production of non-01 v. cholerae isolated from river water, fish and shrimps in kobe city.		1988	3386093
progesterone regulation of secretory component (sc): uterine sc response in organ culture following in vivo hormone treatment.	sex steroid hormones are known to have profound effects on mucosal immunity. in the present study we evaluated the effects of progesterone on the uterine immune system by determining the changes in the levels of secretory component (sc) released from uterine tissues in culture following in vivo administration of progesterone to estradiol-stimulated ovariectomized rats. sc is a transport protein which moves iga into external secretions such as intestinal and uterine secretions. sc release was det ...	1988	3386246