Publications

transport of branched-chain amino acids and their corresponding 2-keto acids by mammalian cells.		1988	3071709
[expression of gene for the vibrio cholerae toxin in escherichia coli].		1988	3073574
7-oxo-pgi2 induced late appearing and long lasting antiischaemic and antiarrhythmic action in dogs.	in our earlier experiments administration of the stable pgi2 analogue: 7-oxo-pgi2-ephedrine salt to dogs resulted in a late-appearing and long-lasting protection from coronary ligation induced ischaemia as well as from postocclusion and reperfusion arrhythmias. objective of the present study was to evaluate the extent and duration of antiischaemic and antiarrhythmic action induced by a single dose 50 ug/kg i.m. 7-oxo-pgi2 in dogs subjected to myocardial ischaemia evoked by left anterior descendi ...	1988	3073767
[the antagonistic activity of lactobacilli relative to cholera vibrios and to non-o1-group cholera vibrios in vitro].		1988	3074247
escherichia coli.		1988	3075217
the role of the ras oncogene in the formation of tumours.	a c-ha-ras 1 oncogene, cloned from the ej human bladder carcinoma cell line, was inserted into a shuttle vector carrying the selectable marker gene gpt that encodes the enzyme xanthine-guanine phosphoribosyl transferase. this construct, psv2gptej, was transfected into nih 3t3 cells by the calcium phosphate precipitation method and cells that had incorporated the plasmid were selected by growth in the presence of mycophenolic acid to which gpt confers resistance. a number of transfectant clones w ...	1988	3075615
the role of the ras oncogene in the formation of tumours.	a c-ha-ras 1 oncogene, cloned from the ej human bladder carcinoma cell line, was inserted into a shuttle vector carrying the selectable marker gene gpt that encodes the enzyme xanthine-guanine phosphoribosyl transferase. this construct, psv2gptej, was transfected into nih 3t3 cells by the calcium phosphate precipitation method and cells that had incorporated the plasmid were selected by growth in the presence of mycophenolic acid to which gpt confers resistance. a number of transfectant clones w ...	1988	3075615
a two-year study of enteric infections associated with diarrhoeal diseases in children in urban somalia.	a hospital-based systematic sample of 1667 children with severe diarrhoeal disease was studied in mogadishu, somalia, throughout 1983 and 1984. one or more enteric pathogens were found in 61% of the patients. rotavirus (25%), enterotoxigenic escherichia coli (11%), shigella spp. (9%), aeromonas hydrophila (9%), giardia lamblia trophozoites (8%), campylobacter jejuni (8%), and vibrio cholerae non-o1 (6%) were the most frequently identified pathogens. age-specific detection rates of enteric pathog ...	1988	3076719
human extraintestinal infections caused by aeromonas species.		1988	3077631
immunoprotective oral whole cell vaccine for enterotoxigenic escherichia coli diarrhea prepared by in situ destruction of chromosomal and plasmid dna with colicin e2.	vaccine regimens which mimic actual infection with bacterial enteropathogens should offer the best opportunity for successful long-term immunoprotection against diarrheal disease caused by enterotoxigenic escherichia coli (etec) or vibrio cholerae. based on this principle, we designed and tested an oral whole cell anti-etec vaccine consisting of intact cells of etec strain h-10407 (st+lt+; o78:h11:cfa/i) which were rendered incapable of replication by treatment with a potent dna endonuclease, co ...	1988	3078575
molecular genetic approaches to the study of vibrio cholerae.	vibrio cholerae o1 is the aetiological agent responsible for cholera, an acute diarrhoeal disease of man, which has occurred in pandemic proportions and still persists. no satisfactory vaccine against cholera is currently in use. this has prompted considerable research on the molecular biology of the organism in order to gain an insight into the variety of its virulence determinants and the mechanisms by which they function and are controlled.	1988	3079181
vibrio cholerae and enteric bacteria in oyster-producing areas of two urban estuaries in australia.	three sampling sites in oyster-producing areas of 2 estuaries were monitored at intervals of about 2 weeks for 1 year. oysters (crassostrea commercialis), water and sediment were examined for vibrio cholerae, escherichia coli and salmonella. v. cholerae was detected in 20, 30 and 11% of oyster, water and sediment samples respectively. the highest incidence was in the autumn (march-may), with few isolations from july to october. most isolates were non-o1 serotypes. the presence of v. cholerae and ...	1988	3079469
antibody to trypanosoma cruzi neuraminidase enhances infection in vitro and identifies a subpopulation of trypomastigotes.	a rabbit antibody to the neuraminidase of the infective form of trypanosoma cruzi identifies a subpopulation of trypomastigotes that expresses neuraminidase. complement-mediated lysis by the antibody selectively destroys 30 to 40% of the trypomastigotes, supporting the conclusion that the immune antibody binds to a subset of parasites. the trypomastigotes that react with the immune antibody are the only ones expressing neuraminidase because the trypomastigotes that survive complement-mediated ly ...	1988	3121750
treatment of multiple sclerosis with various interferons: the cons.	while alpha and beta interferons are relatively nontoxic human gene products and have been used in the treatment of multiple sclerosis, the results of a pilot study in 18 patients treated with recombinant gamma interferon indicate that this form is unsuitable for therapy in ms.	1988	3133576
pathology of stranded beluga whales (delphinapterus leucas) from the st. lawrence estuary, québec, canada.	from june 1983 to may 1986, thirteen carcasses of stranded beluga whales from a polluted area of the st. lawrence river, canada were necropsied. high performance liquid chromatography was performed on the brains of three other animals to determine concentrations of benzo a pyrene (bap). two juvenile animals had severe multisystemic lesions one of which, a severe necrotizing dermatitis, was associated with a herpesvirus-like particle. four adults had five varieties of tumours. an adult had a syst ...	1988	3134469
hiv seroprevalence in migrant and seasonal farmworkers--north carolina, 1987.		1988	3136308
[postoperative debasement of the host-defense system and wound healing in elderly patients, and nutritional support as a therapeutic implication].	plasma opsonic activity and several kinds of plasma protein levels in each pre- and post-operative days were compared between older 25 cases (70 years or older) and 31 younger cases (59 years or younger) which both underwent elective abdominal surgery. the activity to opsonize e. coli 075 as a host-defense capacity was reduced on postoperative days 1,3 and returned gradually in either young or elderly. but opsonic activity levels in elderly patients in each postoperative days were significantly ...	1988	3136311
platelet-activating factor and the cardiovascular system: involvement in cardiac anaphylaxis.		1988	3136455
posttranslational modifications of the ia-associated invariant protein p41 after gene transfer.	biochemical analysis of a rat fibroblast cell clone, transfected with the murine ia associated invariant chain gene, demonstrates the expression of a family of proteins. this indicates that all members of the invariant protein family are derived from the same gene. the proteins p41, ii, p27, p25, and p10, synthesized in the transfectant cell line, are identical with the invariant proteins previously shown to associate noncovalently with ia antigens. these proteins are identified by immunoprecipi ...	1988	3137970
gastroenteritis due to vibrio cholerae el tor ogawa.		1988	3138473
characteristics of polymerized pyridoxylated hemoglobin.	polymerization of sfh-p currently provides the only approach that leads to a hemoglobin solution that approximates the o2 carrying capacity of whole blood and can be infused without altering the cop of plasma. it appears to have an adequate o2 loading and unloading characteristic and a greatly improved intravascular half-life. in addition, stable shelf-life at 4 degrees c of greater than five months, makes it a prime candidate for future pre-clinical and clinical investigations.	1988	3140917
intracellular injection of ca2+ chelator does not affect spike repolarization of cat spinal motoneurons.	both intra- and extracellular injections of tetraethylammonium (tea) prolonged the spike repolarization of motoneurons in the spinal cord of cats under pentabarbitone anaesthesia, but did not depress the afterhyperpolarization (ahp). intracellular injections of egta and the fast-acting ca2+ chelator, bapta, greatly depressed the ahp, but the spike shape remained unchanged. extracellular applications of cd2+ had similar effects. these observations suggest that a ca2+-dependent k+ outward current ...	1988	3141006
restriction endonuclease analysis of brucella abortus.	the restriction endonuclease profiles of bacterial dna from brucella abortus isolates were evaluated. it was not possible to distinguish between vaccine strain 19 and virulent (biotype 1 and biotype 2) strains of b abortus. restriction endonuclease analysis is therefore not a suitable epidemiological tool in bovine brucellosis investigations. the genetic homogeneity of the brucella genus was reinforced by these findings.	1988	3143134
[hemolysin production by vibrio cholerae non-o1 and o1 el tor determined by biological and immunological activities].		1988	3143787
crystals in calcified epiphyseal cartilage and cortical bone of the rat.	the size of hydroxyapatite (hap) crystals in calcified cartilage and cortical bone of the rat has been studied and compared with that of synthetic poorly crystalline hydroxyapatite (pcha). crystal size was determined by x-ray diffraction and selected-area dark field imaging, and their elemental compositions were determined by emission spectroscopy. the crystal size of cartilage, bone, and pcha were found to be between 120 and 170 a in length by 50 a in width as determined by both x-ray diffracti ...	1988	3145127
the effects of norplant-2 rods on clinical chemistry in singaporean acceptors after 1 year of use: haemostatic changes.	a longitudinal study of coagulation parameters was carried out on 100 singapore acceptors using the levonorgestrel subdermal implants norplant-2 rods for contraceptive purposes. at the end of 1 year of use, results from this on-going study indicates that these acceptors have an enhanced potential for hypercoagulation with a significant shortening of their prothrombin time and activated partial thromboplastin time. they also have an increased predisposition to thrombosis as evidenced by significa ...	1988	3145169
is vitellogenin an ancestor of apolipoprotein b-100 of human low-density lipoprotein and human lipoprotein lipase?	vitellogenin, an ancient animal protein, is the major yolk protein of eggs, where it is used as a food source during embryogenesis. here it is shown that vitellogenins, including those from the invertebrates caenorhabditis elegans and drosophila melanogaster, contain domains that are homologous with parts of apolipoprotein b-100 (apob-100) of human low-density lipoprotein and human lipoprotein lipase. as vitellogenins are likely to have been used by invertebrates during embryogenesis well before ...	1988	3145737
effects of excess vitamin a and canthaxanthin on salivary gland tumors.	the effects of feeding semipurified diets supplemented with excess retinyl palmitate (20,000 and 100,000 iu/kg), beta-carotene (250 mg/kg), and canthaxanthin (250 mg/kg) on 7,12-dimethylbenz[a]anthracene (dmba)-induced salivary gland tumors were studied in rats. none of the dietary supplements had a significant effect on tumor incidence. tumor weights, however, tended to be lower in rats fed the dietary supplements compared with the controls. the incidence of tumor-bearing rats with large tumors ...	1988	3146058
[a new sensitivity test for substances used as antiseptics or disinfectants. i. biological studies].	in this first article, the authors present a fast method of determining the bactericide and bacteriostatic power of the germs, based on the disparition of the succino-dehydrogenase activity of a bacterium, objectified by a neotetrazolium chloride reaction. this reaction allows a colorimetric qualitative and quantitative determination of the activity of a preparation and the duration of its contact with the bacteria. this method allows a study in electronic microscopy which will be published in a ...	1988	3146445
tumor necrosis factor and hiv p24 antigen levels in serum of hiv-infected populations.	tumor necrosis factor (tnf) and hiv p24 antigen levels were determined in the serum of intravenous drug abusers (ivdas), homosexuals, and patients with lymphadenopathy or acquired immune deficiency syndrome (aids). the mean tnf level in the serum of normal controls was 12 +/- 5 compared to 112 +/- 25 pg/ml in the serum of hiv-seronegative asymptomatic ivdas. this increase of tnf may be due to the variety of infections that these people are exposed to persistently. the mean tnf level in the serum ...	1988	3146635
characterization of a chimaeric plasminogen activator obtained by insertion of the second kringle structure of tissue-type plasminogen activator (amino acids 173 through 262) between residues asp130 and ser139 of urokinase-type plasminogen activator.	a chimaeric recombinant plasminogen activator (rscu-pa- k2) obtained by insertion of the second kringle (k2) of tissue-type plasminogen activator (t-pa) (amino acids 173-262) between residues asp130 and ser139 of single chain urokinase-type plasminogen activator (scu-pa) was purified from the conditioned medium of mouse myeloma cells transfected with the previously described plasmid pulb9137 (piérard et al., j. biol. chem. 262, 11771-11778, 1987). approximately 22 micrograms of purified protein ...	1988	3146822
[ultrahistochemical demonstration of blood group b substances on human erythrocytes with a lectin from salmo gairdneri rich].	from the roe of salmo gairdneri rich., a lectin was isolated which agglutinates specifically human erythrocytes of blood group b. for cytochemical labelling of the blood group substance b on the surface of human erythrocytes, an indirect approach was chosen. by means of a polyclonal antibody from the rabbit against the b-specific fish lectin, electronmicroscopic presentation was performed with protein a gold using a multistep method. for quantification gold labelling was partially followed by a ...	1988	3146853
cellular immunity after oral administration with v. cholerae.		1988	3147307
on the role of the dorsal noradrenergic bundle in learning and habituation to novelty.	in experiment 1, the performance of vehicle control rats and rats with 6-hydroxydopamine-induced lesions of the dorsal noradrenergic bundle (db) was examined in acquisition and extinction of bar pressing and in spontaneous and food-reinforced alternation in a t-shape maze. plasma corticosterone levels in basal conditions, after chronic food restriction, after transportation to a novel environment, and after sessions of either rewarded or nonrewarded bar pressing were assayed. db lesions produced ...	1988	3147459
the chlordiazepoxide/pentylenetetrazol discrimination: characterization of drug interactions and homeostatic responses to drug challenges.	rats were trained to discriminate chlordiazepoxide (cdp) from pentylenetetrazol (ptz) in a two-lever food motivated discrimination task. training drug doses were adjusted until subjects emitted approximately 50% of their responses on each of the two drug-appropriate levers during saline injection tests. tests that followed injection of cdp/ptz combinations illustrated a reciprocal antagonism between the two drugs. saline-injection tests that followed large dose injections of cdp revealed a perio ...	1988	3147473
nicotine tolerance: an analysis of the time course of its development and loss in the rat.	the time course of the development and loss of tolerance to nicotine was measured in female rats that were injected subcutaneously (sc), twice daily with 1.6 mg/kg nicotine. tolerance to nicotine-induced decreases in locomotor activity and body temperature were observed. tolerance to the effects of nicotine on both of these measures developed rapidly, with maximal changes occurring within 2-4 days after initiation of treatment. the binding of l-[3h]-nicotine was measured in six brain regions. ch ...	1988	3147478
[transperitoneal transport of methotrexate: in vitro studies].		1988	3147495
[the laboratorio nacional de referencia de mycobacteria y tuberculosis in the antituberculosis campaign in cuba].		1988	3147500
the venous blood-flow of the flexor aspect of the human forearm. clinical relevance to the distally-pedicled forearm flap.	in the distally-pedicled radial island flap a reverse venous flow was observed. in 20 cadaver extremities the veins of the lower arms were perfused and dissected and the island flaps elevated. the 3 venous systems are described here according to the number, site and shape of the valves and the venous flow from the flap is also discussed.	1988	3147536
the simavi-tanga project for self-help in health. an assessment of a comprehensive primary health care programme.	primary health care (phc) is the major strategy for achieving the goal of health for all. lessons are to be learnt from a variety of experiences. the simavi-tanga project for self-help in health is a comprehensive phc-project with a limited input of external financial and advisory assistance. the short term outcome in terms of effectiveness and efficiency does not differ essentially from comparable tanzanian projects with a higher degree of external input. the main limiting factors with regard t ...	1988	3147549
the effect of hypophysectomy on inhibin production by adult rat ovaries: changes in ovarian inhibin gene expression and serum inhibin.	we have investigated the effect of hypophysectomy on adult rat ovarian inhibin alpha- and beta a-subunit mrna, serum inhibin, and circulating gonadotrophin levels. immediate declines in ovarian inhibin gene expression and serum inhibin were observed following hypophysectomy, with a nadir on day 4. however, inhibin synthesis determined by these parameters was still apparent at 16 days post-hypophysectomy. gonadotropin levels were suppressed following hypophysectomy and the fall in inhibin product ...	1988	3147584
the development of immunological relationship between mother and fetus under physiological and pathological conditions.	on the basis of results of our research and review of literature, the complex of immuno logical influences, operating during the development of the human fetus, were evaluated. it is obvious that during the early stages of pregnancy the conceptus is protected by non-specific mechanisms, i.e. hormonally (hcg, progesterone) and by certain properties of the trophoblast (barrier function, immunologically inert surface). specific immunological tolerance is formed by gradual penetration of trophoblast ...	1988	3147589
primate models for evaluation of aids vaccines.		1988	3147661
an electron microscope study of the development of the exocrine and endocrine pancreas with special reference to intercellular junctions.	intercellular junctions in pancreatic acinar, duct and endocrine cells were studied by thin section and freeze-fracture methods in developing rats and mice. undifferentiated cells were joined by the zonula occludens and isolated fragments of tight junctional strands. small gap junctions were either occasionally associated with tight junctional strands or appeared independent of them. during the morphological differentiation of acinar cells, strands of the zonula occludens developed to form a com ...	1988	3147689
isolation of the early phase of chylomicron formation in intestinal epithelial cells of rats.	lipid is first observed electron microscopically in the rough endoplasmic reticulum of intestinal epithelial cells during active lipid absorption. we have been able to isolate this subcellular fraction by using discontinuous sucrose gradients of 0.25/0.86/1.11 m sucrose. a preliminary low speed centrifugation of mucosal homogenate removed the heavier subcellular organelles. the resulting supernatant was centrifuged at 5.25 x 10(6) x g.min. the pellet from this centrifugation was placed on top of ...	1988	3147719
characterization of the priming effects of human granulocyte-macrophage colony-stimulating factor on human neutrophil leukotriene synthesis.	human granulocyte-macrophage colony-stimulating factor (gm-csf) is an in vitro and in vivo stimulator of human bone marrow myelomonocytic precursor cells and mature granulocyte and macrophage effector cells. we have compared the effect of gm-csf on the synthesis of 5-lipoxygenase products induced by the chemotactic peptide fmet-leu-phe and the calcium ionophore a23187 in human neutrophils. although gm-csf alone did not stimulate detectable synthesis of products of the 5-lipoxygenase pathway, pre ...	1988	3148964
induction of differentiation of human promyelocytic leukemia cells (hl60) by metabolites of hexamethylene bisacetamide.	we studied the ability of five metabolites of hexamethylene bisacetamide (hmba), which we had previously identified in patient urine, to induce differentiation or to influence differentiation induced by hmba of a human promyelocytic cell line. differentiation of hl60 cells was quantified by morphological changes and by the ability to reduce nitroblue tetrazolium. n-acetyl-1,6-diaminohexane (nadah), the deacetylated, first metabolite of hmba, was a more potent inducer of hl60 differentiation than ...	1988	3163938
[comparative study of physico-chemical and biological properties of human somatotropin produced by genetic engineering and isolated from the pituitary gland].	human somatotropin hormono (sth), produced by means of gene engineering in the complex program "human growth hormone", managed by the academy of sciences of the ussr, ministry of medical and biological industry of the ussr and ministry of public health of the ussr, was shown to be similar in its physico-chemical properties to the main isoform of highly purified sth, isolated from human hypophysis. as distinct from the hypophyseal sth (sthhyp) containing minor isoforms of the hormone, the prepara ...	1988	3165230
structural and functional studies of the effects of sympathetic nerve stimulation on rabbit submandibular salivary glands.	continuous sympathetic stimulation at 8-10 hz caused intense vasoconstriction in the gland, so stimulation was generally given in an interrupted pattern to minimize this detrimental effect on secretion. only a small increase in fluid secretion occurred; it became thick and tended to block the cannula; therefore in later experiments the main duct was not cannulated. after sympathetic stimulation there was substantial degranulation of acinar cells. however, as this was accompanied by little moveme ...	1988	3165262
failure to find htlv i and hiv i serum antibodies in ms patients.		1988	3165371
[an immunocytochemical study on the prostaglandin e2 localization in human gastric mucosa].		1988	3165480
yeast activators stimulate plant gene expression.	gal4 is a transcriptional activator found in yeast. two distinct functions of the protein are required for its activity: one directs sequence-specific dna binding, and another interacts with some other component of the transcriptional machinery, for example, rna polymerase ii or a tata-binding protein. two short regions of gal4 function as 'activating sequences' when attached to the dna-binding portion of gal4 and these regions can be replaced by a large number of peptides encoded by escherichia ...	1988	3165494
atypical mycobacterial infections of hickman catheter exit sites.		1988	3165525
[guidelines for the management of patients infected by the human immunodeficiency virus].		1988	3165542
an interstitial deletion of chromosome 7 may characterize a subgroup of uterine leiomyoma.		1988	3165693
ring chromosome 6 and i(17q-) in a patient with acute promyelocytic leukemia. absence of translocation t(15;17).		1988	3165701
chondrogenesis and osteogenesis of bone marrow-derived cells by bone-inductive factor.	rat bone marrow cells were intraperitoneally implanted within a diffusion chamber with a decalcified bone matrix or a 4 m guanidine hydrochloride extracted matrix (g-res) as control. the chamber was harvested after 28 days and soft x-ray photography, histological examination, determination of alkaline phosphatase activity and calcium content were performed. with the decalcified bone matrix, cartilage and bone formation was observed and both alkaline phosphatase activity and calcium content were ...	1988	3166833
interaction of n-acetyl-4-epi-d-neuraminic acid with key enzymes of sialic acid metabolism.	in spite of the axially orientated hydroxy group at c-4, the benzyl alpha-glycoside of n-acetyl-4-epi-d-neuraminic acid (4-epi-neuac) is a substrate for sialidases from vibrio cholerae, clostridium perfringens, and arthrobacter ureafaciens, although to an extent which differs depending on the enzyme. surprisingly, v. cholerae sialidase is by far the slowest acting enzyme; this is in contrast to its usual behavior. fowl plague virus sialidase and bovine testis sialidase also cleave this glycoside ...	1988	3166979
structure of a ganglioside with cad blood group antigen activity.	the cad antigen is a rare erythrocyte blood group antigen expressed on both sialoglycoprotein and ganglioside structures. it is related both serologically and biochemically to the sda blood group antigen expressed on over 90% of caucasian erythrocytes. we reported previously that cad erythrocytes contain a novel ganglioside that binds helix pomatia lectin and inhibits human anti-sda antibody. we have now purified the cad ganglioside and determined its structure. the ganglioside contained glc-gal ...	1988	3167001
[specific binding of phenol-containing antioxidant with plasma membranes of cells and its inhibition by various biologically active substances].		1988	3168734
tear secretion and lacrimal gland function of rabbits treated with isotretinoin.	adverse ocular reactions including dry eye symptoms and blepharoconjunctivitis are common side effects of treatment with isotretinoin (13-cis-retinoic acid). however, there is little agreement in the literature on the effect of this drug on the tears. because we have previously shown that the lacrimal gland secretes isotretinoin, we conducted a study of the effect of isotretinoin on lacrimal gland function. rabbits were treated with isotretinoin for 5 months. throughout the study tear secretion ...	1988	3170794
tear secretion and lacrimal gland function of rabbits treated with isotretinoin.	adverse ocular reactions including dry eye symptoms and blepharoconjunctivitis are common side effects of treatment with isotretinoin (13-cis-retinoic acid). however, there is little agreement in the literature on the effect of this drug on the tears. because we have previously shown that the lacrimal gland secretes isotretinoin, we conducted a study of the effect of isotretinoin on lacrimal gland function. rabbits were treated with isotretinoin for 5 months. throughout the study tear secretion ...	1988	3170794
[comparative study of the nociceptive reactions when bradykinin is administered in different receptor areas to waking animals].		1988	3174259
the metabolic response to skin temperature.	experiments were done to assess that fraction of the metabolic response to external cold exposure, which is attributable to skin temperature. in 5 conscious and closely clipped goats the metabolic rate was determined at various stable levels of skin temperature in the range from 13 to 41 degrees c, while core temperature was kept constant at 38.8 degrees c. skin temperature was manipulated by a rapidly circulating shower bath, while core temperature was controlled by means of heat exchangers act ...	1988	3174397
receptor-like function of heparin in the binding and uptake of neutral lipids.	molecular mechanisms regulating the binding, amphipathic stabilization, and metabolism of the major neutral lipids (e.g., cholesteryl esters, triglycerides, and fatty acids) are well studied, but the details of their movement from a binding compartment to a metabolic compartment deserve further attention. since all neutral lipids must cross hydrophilic segments of plasma membranes during such movement, we postulate that a critical receptor-like site exists on the plasma membrane to mediate a ste ...	1988	3174644
characterization of the phage-specific transfer rna molecules coded by cholera phage phi 149.	aminoacylation of trna isolated from choleraphage phi 149-infected cells with individual 3h-labeled l-amino acids followed by hybridization with phage dna revealed that the phage encodes trnas specific for arginine, proline, glycine, isoleucine, serine, valine, tyrosine, histidine, lysine, leucine, tryptophan, and aspartic acid. aminoacylation of phage-coded trnas isolated from phage dna-rna hybrids also confirmed this observation except for tryptophan.	1988	3176346
effects of catecholestrogen 2-monomethyl ether on serum cholesterol and lipoprotein levels.		1988	3178584
parachuting injuries and type of parachute in a reserve rescue unit.	over a 57-month period, 3,246 parachute jumps were performed in an air rescue reserve unit. during this time, the unit transitioned from a round static line parachute to a rectangular free falling parachute. the rates of 11 injuries per 1000 jumps and 600 lost man days per 1000 jumps were reduced to zero after the introduction of the new parachute.	1988	3178632
phasic activity of human dorsal foot veins.	phasic contractions of the dorsal foot veins were detected in 7 male subjects, aged 23 to 49 years, by means of a linear variable differential transformer (lvdt) after congesting the veins by a pressure of 6.7 kpa (50 torr). this phasic activity (pa) had a frequency of 3 to 7 contractions per minute and led to a periodic diminution of the diameter of the distended veins by 12 to 44%. using a double lvdt, the pa was shown to represent peristaltic waves travelling along the vessel wall concomitant ...	1988	3178793
characterization of a colon carcinoma cell line for tumor immunotherapy.	chessboard vaccination (i.d. injection of various mixtures of mitomycin-treated fresh cells of the de-ta colon carcinoma cell line and vibrio cholerae neuraminidase (vcn] had a beneficial effect on recurrence and survival in duke c patients. to standardize this kind of immunotherapy the following parameters of the de-ta cell have been evaluated:--karyotype (46 chromosomes, deletions in chromosome 8; 17); doubling time 24 hr; expression of cea.--stability of membrane antigens characterized by 9 d ...	1988	3180140
transient involvement of enkephalins in both the sympathetic and parasympathetic innervations of the submandibular gland of rats. light- and electron-microscopic immunocytochemical study.	a time course study with enkephalin (enk)-like immunoreactivity has revealed that nerve fibers intensely immunoreactive for enk-8 appeared transiently only during the postnatal week 2 and 4 within the acini as well as in the inter- and intralobular connective tissues of the submandibular gland of rats. at these stages numerous nerve fibers immunoreactive for tyrosine hydroxylase (th) appeared also in the inter- and intralobular connective tissues and within the acini. coincidently with these pos ...	1988	3180183
identification of inflammatory cells in bovine milk by flow cytometry.	cells recovered from normal or mastitic bovine milk were examined by flow cytometry. all milk samples contained particulate material that was heterogeneous in size and that produced a right-angle light-scatter signal equal to or greater than that produced by human or bovine neutrophils. although this material labeled with hoechst 33342, it produced fluorescence intensities below that of intact bovine cells, suggesting that it consisted of cell fragments. mastitic milk additionally contained othe ...	1988	3180948
changes in the epidemiological pattern of mycoplasma pneumoniae infections in denmark. a 30 years survey.	a seroepidemiological survey has shown a remarkable shift in the previously reported regular cyclic pattern of mycoplasma pneumoniae epidemics which culminated every 4.5 years during the period 1958-74. the last of four regular epidemics occurred in 1972. it was followed by 'premature' epidemics in 1975 and 1977/8 which inaugurated a change from an epidemic to an endemic pattern of the infection in denmark. over the following 9 years (1978-86) there has been an irregular but significant decrease ...	1988	3181319
the comparative in-vitro activity of ofloxacin.	the antibacterial activity of ofloxacin, a new fluoroquinolone, was evaluated against a wide range of clinical bacterial isolates and compared with that of nalidixic acid, norfloxacin, enoxacin, pefloxacin and ciprofloxacin by determination of minimum inhibitory concentrations (mics). ofloxacin was very active against nalidixic acid-susceptible isolates of the enterobacteriaceae (mic less than or equal to 0.12 mg/l) and was also active against strains resistant to nalidixic acid (mic less than o ...	1988	3182468
intracellular trafficking of cell surface sialoglycoconjugates.	recent reports have suggested that the majority of the molecular traffic through the golgi apparatus is comprised of recycling, rather than newly synthesized, molecules. to evaluate the importance of this recycling pathway in greater detail, we examined the internalization and recycling of cell surface glycoproteins on el-4 cells, a murine t-cell lymphoma, using sialic acids as covalent markers. sialic acids were removed from the surface of living cells by exhaustive treatment with vibrio choler ...	1988	3182795
coagglutination of vibrio cholerae, vibrio mimicus, and vibrio vulnificus with anti-flagellar monoclonal antibody.	monoclonal antibodies (mabs) with serological activity for purified flagellar (h) core protein prepared from vibrio cholerae were identified by enzyme-linked immunosorbent assay. four of these mabs reacted with the flagella of v. cholerae and v. mimicus exclusively, while eight mabs reacted with at least 1 of 30 heterologous vibrio species tested by enzyme-linked immunosorbent assay or coagglutination. it appears that v. cholerae and v. mimicus express similar, if not identical, h determinants u ...	1988	3182988
effects of dietary casein and soybean protein on triglyceride turnover in rat liver.	we previously found that hepatic lipogenic enzyme induction, fatty acid synthesis, and triglyceride level were markedly lower in rats fed soybean protein than in those fed casein (iritani et al. (1986): j. nutr., 116: 190). after labeling triglycerides with tritiated water, the effects of dietary protein on the triglyceride degradation have been investigated. after the injection of tritiated water into rats, the radioactivities of fatty acids and triglycerides reached a plateau in 1-2 days and w ...	1988	3183779
organization of a complex movement: fixed and variable components of the cockroach escape behavior.	the escape behavior of the cockroach periplaneta americana was studied by means of high speed filming (250 frames/s) and a computer-graphical analysis of the body and leg movements. the results are as follows: 1. the behavior begins with pure rotation of the body about the posteriorly located cerci, followed by rotation plus forward translation, and finally pure translation (figs. 1, 2). 2. a consistent inter-leg coordination is used for the entire duration of the turn (fig. 3a). at the start of ...	1988	3184004
the effect of various antibacterial preparations on the in vitro morphology and chemotactic response of equine neutrophils.	two independent assay systems were used to study the effect of three antibacterial preparations on in vitro morphology and chemotaxis of equine neutrophils. incubation of neutrophils with high (200 micrograms/ml) and medium (20 micrograms/ml) concentrations of neomycin impaired their response to standard chemoattractants. trimethoprim/sulfadoxine (0.4/2.0 micrograms/ml-40/200 micrograms/ml) and benzylpenicillin (0.25-25 micrograms/ml) had no effect. neutrophils collected from geldings 2 and 24 h ...	1988	3184258
heterogeneous colony forming ability in vitro of the abnormal clone-derived granulocyte-macrophage precursors in myelodysplastic syndromes.	the clonal origin of granulocyte-macrophage colony forming cells (cfu-gm) in the myelodysplastic syndrome (mds) was cytogenetically studied. chromosome analysis was carried out on single gm-colonies from six patients with mds, whose bone marrow cells had chromosome abnormalities. abnormal clone-derived cfu-gm were grown in four patients under the presence of human placental conditioned medium. in the remaining two, all analysed colonies revealed a normal karyotype, although the majority of metap ...	1988	3184986
aflatoxin contamination of us corn.		1988	3185703
[resuscitation of severe meningitis in infants and children].	meningeal penetration of bacteria induces an inflammatory response which affects mainly the endothelium of cerebral vessels. this inflammatory reaction is directly responsible for thrombosis and indirectly creates cerebral oedema and reduced cerebral blood flow. cerebral ischemia is the result of all these phenomenons. nowadays, control of cerebral oedema is the main goal of intensive care treatment of meningitis that needs invasive monitoring of intracranial pressure. we may hope that in a next ...	1988	3186420
thyroid hormone deficiency and muscle metabolism during light and heavy exercise in dogs.	muscle glycogen, glycolytic intermediate and high energy phosphate contents were compared in 5 intact-control (c) and thyroidectomized (thy) dogs after 30 min treadmill exercise of low (40w) and high (70w) intensities. although after the exercise of relatively low intensity the rate of glycogenolysis and muscle lactate accumulation in thy dogs exceeded those in controls, the diminished oxidative capacity in the former was inadequately compensated, resulting in lowering of apt and crp contents. a ...	1988	3186437
reinnervation of skin by polymodal nociceptors in rats.		1988	3187035
efferent modulation of penile mechanoreceptor activity.		1988	3187039
research on heterocyclic compounds. xxiv. antiinflammatory activity of some imidazo[1,2-c]pyrimidine derivatives.	a group of four acidic imidazo[1,2-c]pyrimidine derivatives were subjected to a series of tests in vivo in order to assess their pharmacological activity. antiinflammatory activity was studied by means of the carrageenin-induced paw edema and pleurisy in rats. hot plate test and writhing induced by acetic acid in mice were employed to evaluate analgesic activity, whereas the yeast-induced hyperthermia in rats was used to study antipyretic activity. the irritative and ulcerogenic action on the ra ...	1988	3187191
[interserovar antitoxic immunity to vibrio cholerae toxins].	the immunochemical affinity of v. cholerae enterotoxins, serovars inaba and ogawa, has been shown in animal experiments on cross antitoxic immunity in the small intestine, the passive hemagglutination test and the toxin neutralization test. however, antitoxic interaction with both enterotoxins is characteristic only for antibodies to v. cholerae of serovar inaba, while in animals immune to ogawa toxin the choleragenic effect of enterotoxins produced by v. cholerae of both serovars in retained. t ...	1988	3188734
effect of sexual maturity on testicular injury subsequent to procarbazine administration in rats.	the present study examined the effect of age on various aspects of leydig cell and sertoli cell function in sprague-dawley rats administered procarbazine. procarbazine was administered intraperitoneally to sprague-dawley rats aged 14, 24, and 60 days in 3 weekly injections of 200 mg/kg. animals were sacrificed 1 week after the last injection. severe impairment of spermatogenesis was evident in all animals. sertoli cell function, as assessed by total testicular abp content, was not significantly ...	1988	3190359
structural studies on oncofetal carbohydrate antigens (ca 19-9, ca 50, and ca 125) carried by o-linked sialyloligosaccharides on human amniotic mucins.	mucins were extracted from human amniotic fluid in the presence of 45% vol. phenol and separated from the bulk of smaller-sized glycoproteins by exclusion on sephacryl s400. the mucin-fraction fw, which still contained a minute proportion of mannose, strongly expressed oncofetal antigens recognized by monoclonal antibodies c 50, ns 19-9, oc 125, leu m1, 49 h 8, and 115 c 2. the structures of the respective mucin-linked saccharides responsible for ca 50-, ca 19-9-, and lea-related antigenic activ ...	1988	3191509
interrelationships between renal tca-cycle metabolism and ammoniagenesis: studies with 13c-nmr and gc-ms.		1988	3191718
mouse centromeric heterochromatin: isolation and some characteristics.	a method is suggested for isolation of highly purified mouse centromeric heterochromatin. treatment of mouse liver nuclei with decreasing concentrations of ca2+ resulted in the gradual unraveling of chromatin in the nucleus and at 0.1 mm ca2+ electron microscopy revealed several dense particles per nucleus, surrounded by decondensed chromatin. these particles, assumed to represent centromere regions of interphase chromosomes by in situ hybridization with radioactive mouse satellite dna and by di ...	1988	3191953
[the role of cholinergic processes in realizing the analgesic effect of gaba-positive preparations].	in experiments on male rats it was shown that scopolamine diminished while pilocarpine and physostigmine potentiated the antinociceptive effect of baclofen, gamma-acetylenic-gaba, thip and depakine. at the same time the antinociceptive effect of pilocarpine virtually underwent no changes in baclofen- and depakine-tolerant animals. the results suggest that the cholinergic processes may be involved in gabaergic antinociception but not in the development of tolerance to the antinociceptive effect o ...	1988	3191968
[analysis of the hypothermic action of etimizol].	in chronic experiments on rabbits it was shown that the hypothermic effect appears in the series of antipheins after the addition of ethyl radical in the first position of imidazole ring. the hypothermic effect of etimizol is more pronounced in the brain than in the body, whereas caffeine equally increases the temperature of the brain and body.	1988	3191969
[fluorescence dynamics of anilino naphthalenesulfonate in mitochondria exposed to corticosteroids].	anilino naphthalenesulfonate fluorescence dynamics in the rat liver mitochondria during the first six minutes after corticosteroid (corticosterone, cortisone, hydrocortisone, dexamethasone) and cholesterol treatment in vitro was studied. the biphasic character of the process was observed depending on the hydrophobicity and concentration of the steroids.	1988	3191977
[diethylstilbestrol potentiation of the antiandrogenic effects of niftolide in the prostate].	in experiments on intact mature male wistar rats it was shown that a combined administration of niftolide (2.5, 5 and 10 mg/kg) and diethylstilbestrol (10 and 50 mg/kg) produced a more pronounced antiprostatic effect than their separate administration.	1988	3191980
chicken liver basic fatty acid-binding protein (pi = 9.0). purification, crystallization and preliminary x-ray data.	chicken liver basic fatty acid-binding protein (pi = 9.0) has been purified with a high yield by a modification of a method originally applied to rat liver. the final product is highly homogeneous and can be used to grow crystals that belong to two different space groups. the crystals are either tetragonal, space group p4(2)2(1)2 with a = b = 60.2 a and c = 138.1 a or orthorhombic, space group p2(1)2(1)2(1) with a = 60.7 a, b = 40.1 a and c = 66.7 a. the second form appears to be more suitable f ...	1988	3191992
polymeric and monomeric iga response in serum and milk after parenteral cholera and oral typhoid vaccination.	the effect of vaccinating lactating pakistani mothers with a combination of live oral typhoid vaccine and parenteral inactivated cholera vaccine on specific milk and serum iga antibodies in both monomeric (m) and polymeric (p) forms was analysed. iga antibody titres peaked for both antigenic specificities 2 weeks after the first dose of vaccine. 82 +/- 7% of anti-vibrio cholerae and 72 +/- 17% of anti-salmonella typhi iga were in the polymeric form. these serum piga antibodies were mainly dimeri ...	1988	3194704
the effect of somatostatin analogue (sms 201-995) on pancreatic blood flow.	the effect of somatostatin analogue sms 201-995 on pancreatic blood flow was studied. in 24 dogs all vessels to the pancreas, except for the pancreaticoduodenal artery and vein, were divided. a flow probe was placed around the pancreaticoduodenal artery. the animals were divided into four groups. control animals received a subcutaneous injection of 0.5 ml of normal saline solution. treated animals received 0.002, 0.02, and 0.2 mg/kg of sms 201-995 at the outset of the experiment. mean systemic a ...	1988	3194830
the distribution of podosomes in osteoclasts cultured on bone laminae: effect of retinol.	osteoclasts, isolated and purified from the medullary bone of calcium-deficient egg-laying hens, adhere to glass coverslips in vitro by means of specialized protrusions of the ventral membrane, denoted podosomes. these structures represent dotlike close-contact adhesion sites in which most cytoskeletal proteins involved in linking the plasma membrane to microfilaments are organized according to a specific and previously described pattern also shared by many oncogene-transformed cells. we show no ...	1988	3195364
epidemiology of antimicrobial resistant cholera in kenya and east africa.	strains of vibrio cholerae o1, el tor resistant to multiple antimicrobial agents, were isolated in kenya between 1982 and 1985. strains of serotype ogawa were resistant to tetracycline, ampicillin, and trimethoprim/sulfamethoxazole. resistance was mediated in all instances by a plasmid ca 100 md of incompatibility group c. based on analysis of restriction endonuclease digests, all ogawa isolates had an identical resistance plasmid. this plasmid differed from plasmids in resistant v. cholerae o1 ...	1988	3195696