Publications

[effect of vestibular stimulation on indices of protein and vitamin metabolites].		1989	313123
circulating erythropoitic precursors in patients with aplastic anemia and chronic granulocytic leukemia.		1989	286494
[nurses from soweto - in south africa we're just a nothing].		1989	247695
factors influencing bacterial production of inducers of settlement behavior of larvae of the oystercrassostrea gigas.	dissolved chemical inducers of settlement behavior of veliger larvae of the oystercrassostrea gigas are found in supernatants of both pigmented species of bacteria (alteromonas colwelliana, vibrio cholerae strain htx) as well as nonpigmented bacteria (excherichia coli, vibrio cholerae strain 596-b). usually less than 10% of veligers exhibited settlement behavior in response to supernatants from the early bacterial growth phases, whereas 30-90% of larvae responded when exposed to supernatant from ...	1989	24197287
mechanisms of diarrhoea and why they matter.	diarrhea involves a significant increase in excreted fluid volume, due either to a failure of the bowel to reabsorb or absorb fluid or to a great increase in fluid secreted into the bowel. to cause diarrhea, pathogenic organisms must be swallowed, survive the acid in the stomach, colonize the small bowel, and stick to the enterocytes. in secretory diarrhea, vibrio cholerae bacteria and enterotoxigenic escherichia coli enter the enterocytes, stimulate adenylatecyclase, and cause a chain of reac ...	1988	12281645
production and partial characterization of pili on non-o1 vibrio cholerae.		1988	2891773
isms positions on aids and hiv antibody testing.		1988	2895089
dietary effects on initiation and promotion of hepatocarcinogenesis in rat.	female f344/n rats were initiated with den (10 mg/kg) 24 h after a 70% partial hepatectomy. groups of 10 rats were fed (a) ain, group-1; (b) pd, group-2; or (c) nih, group-3, for 1 week after initiation and were then fed nih plus the promoting agent pb at a level of 0.05% in the diets for 6 months. other groups were fed nih for 1 week after initiation and then nih without pb (group-4), ain + pb (group-5), ain without pb (group-6), pd + pb (group-7), or pd without pb (group-8) for 6 months. the n ...	1988	2895112
an outbreak of campylobacter jejuni infection in a neonatal intensive care unit.		1988	2895144
one-minute endoscopy room test for campylobacter pylori.		1988	2895232
sex as a risk factor for htlv-i spread among intravenous drug abusers.		1988	2895250
comparison of adrenal medullary, carotid body and pc12 cell grafts in 6-ohda lesioned rats.	the survival and functional properties of dispersed cell implants of catecholaminergic cells obtained from the peripheral nervous system of adult rats (adrenal medulla and carotid body glomus cells) and pc12 cells from a rat pheochromocytoma cell line were examined following transplantation into the striatum of the adult rat. the host animals, all with unilateral 6-hydroxydopamine (6-ohda) nigrostriatal lesions, were divided into 5 groups: (1) pc12 cells transplanted into cyclosporin-a treated h ...	1988	2896533
antimicrobial susceptibility of bordetella pertussis (part i).	in this review of the literature data are collected from the more recent studies on the susceptibility of bordetella pertussis to penicillins, cephalosporins, other beta-lactam antibiotics, aminoglycosides, tetracyclines, erythromycin, josamycin, co-trimoxazole and various other antibiotics. the methods of susceptibility testing of b. pertussis are discussed and suggestions for standardization are made.	1988	2897337
use of ultrasonography to diagnose sertoli cell neoplasia and cryptorchidism in a dog.	unilateral cryptorchidism and sertoli cell tumor in the descended testis were provisionally diagnosed by use of ultrasonography in a 7-year-old keeshond. the left testis was thought to have degenerated, but ultrasonography of the scrotum did not reveal evidence of testicular or epididymal tissue. the contralateral testis contained a hypoechoic mass believed to be a testicular tumor. surgery and histopathologic findings confirmed the diagnosis. ultrasonography of the scrotum and its contents shou ...	1988	2897351
arylamine n-acetyltransferase from chicken liver ii. cloning of cdna and expression in chinese hamster ovary cells.	a cdna clone encoding the full coding region of chicken liver arylamine acetyltransferase (ec 2.3.1.5) was isolated from lambda gt10 cdna library by screening with the 32p-labeled oligonucleotide deduced from the amino acid sequence reported in the preceding paper. the complete nucleotide sequence of the cdna was determined, from which the amino acid sequence of n-acetyltransferase was deduced. it consisted of 1,302 nucleotides including a 861-nucleotide region coding for 287 amino acids with a ...	1988	2897360
steroid regulation of somatostatin mrna in the rat hypothalamus.	the participation of gonadal steroid hormones in the regulation of the expression of the somatostatin gene in the hypothalamus and cerebral cortex was studied by using a quantitative densitometric hybridization assay which allows the direct measurement of specific somatostatin mrna levels. the levels of somatostatin mrna in hypothalamus were found to be significantly decreased following gonadectomy in both male and female rats (67% in males and 75% in females). moreover, with in situ hybridizati ...	1988	2897361
fibronectin is a component of the sodium dodecyl sulfate-insoluble transglutaminase substrate.	liver plasma membranes contain a morphologically distinct protein complex which serves as a substrate for the plasma membrane-associated transglutaminase. the complex, which appears as a two-dimensional sheet, is insoluble in sodium dodecyl sulfate and reducing agents and has been named sits for sodium dodecyl sulfate-insoluble transglutaminase substrate (tyrrell, d. j., sale, w. s., and slife, c. w. (1988) j. biol. chem. 263, 1946-1951). polyclonal antibodies raised against sits were used to pr ...	1988	2897368
thin-layer chromatographic screening method for the tranquillizers azaperone, propiopromazine and carazolol in pig tissues.	a procedure is described for the detection of azaperone, propiopromazine and carazolol in pig muscle, liver and kidney tissue. the method comprises extraction from an alkaline tissue homogenate with diethyl ether, followed by cleaning up and concentration of the extract on a silica gel solid-phase extraction column. two-dimensional thin-layer chromatography on a silica plate was used for the detection of the tranquillizers. detection levels were 25 micrograms kg-1 for propiopromazine, 50 microgr ...	1988	2897377
supraspinal nocifensive responses of cats: spinal cord pathways, monoamines, and modulation.	these experiments were conducted to determine (1) whether dorsal and ventral ascending spinal pathways can each mediate unlearned supraspinal nocifensive responses of cats to noxious thermal stimuli and (2) whether interrupting the spinal projection of supraspinal monoaminergic neurons alters the excitability and natural modulation of these responses. in partially restrained cats, thermal pulses (greater than or equal to 47 degrees c) delivered to the hindlimbs of intact cats or rostral to lesio ...	1988	2897383
pemphigus foliaceus-like, immunologically negative dermatosis in a patient with t cell chronic lymphocytic leukemia.	a 56-year-old black man developed a pemphigus foliaceus-like bullous eruption as the initial presentation of chronic t cell lymphocytic leukemia. histologic examination disclosed superficial acantholysis consistent with pemphigus foliaceus and an infiltrate of atypical lymphoid cells in the papillary dermis. repeated direct and indirect immunofluorescence studies yielded negative results. acantholysis could be reproduced in vitro by incubation of normal human skin with the patient's serum and pl ...	1988	2897384
phenotypic and functional characterization of human contrasuppressor cell interactions.		1988	2897409
identification of a unique lymphocyte subpopulation in the sheep uterus.	a panel of monoclonal antibodies was used to define the lymphocyte subpopulations in the sheep uterus at various stages of the oestrous cycle. a striking finding was that the majority of lymphocytes in the uterine and endometrial glandular epithelia belonged to a unique lymphocyte subpopulation that expressed the cd45r antigen but was negative for major histocompatibility complex (mhc) class ii molecules and expressed low or undetectable levels of the cd5 antigen. when examined under the electro ...	1988	2962930
effect of route of administration on the pharmacokinetics of etretinate in the dog.		1988	2963666
atrial natriuretic factor during head-out immersion at night.	the present investigation was undertaken to test the hypothesis that a circadian variation in the atrial natriuretic factor (anf) response is responsible for the nocturnal inhibition of renal responses to head-out immersion (hoi). plasma anf responses to a 3-h hoi (water temperature 34.5 +/- 0.5 degrees c) were studied during day (1000-1300) and night (2400-0300) in six hydropenic male human subjects. in agreement with the previous observations, the renal responses, especially the diuresis, to h ...	1988	2964206
evidence for in vitro regulation of ige receptors on the human basophil membrane by their removal and reexpression: effects of ca2+, mg2+, some metabolic inhibitors and fetal calf serum.	human basophils free of receptor-bound ige and suspended in rpmi-1640 + 0.2% edta or in a buffered salt solution lacking ca2+ and mg2+ bound optimal quantities of 125i ige. in media containing ca2+ and mg2+ such as rpmi-1640 alone or a buffered physiologic salt solution binding was reduced significantly in the first 60 min and remained essentially unchanged in the next 120 min. scatchard analysis showed the reduction to be due both to loss of number and affinity of receptors. the presence of bot ...	1988	2965111
[cyclosporin a inhibits arachidonic acid metabolism in cultured cells].		1988	2978254
the effect of an acute maternal stress on beta-endorphin and growth hormone releasing factor in the rat fetus.	pregnant rats were injected with saline or l-tyrosine methylester hcl (200 mg/kg) and subjected to an acute forced immobilization stress on day 20 of gestation. at 10, 30, 60, and 120 minutes after the onset of stress, their fetuses were dissected out, and the contents of hypothalamic and pituitary immunoreactive beta-endorphin (ir-beta-ep) and hypothalamic immunoreactive growth hormone-releasing factor (ir-grf) were determined by specific radioimmunoassays. the maternal stress arose a significa ...	1988	2967192
iron-regulated hemolysin production and utilization of heme and hemoglobin by vibrio cholerae.	el tor and non-o1 strains of vibrio cholerae were analyzed to determine whether synthesis of secreted hemolysin was influenced by the concentration of iron in the medium. synthesis of hemolysin was found to be iron regulated in both el tor and non-o1 isolates. increased levels of hemolytic activity were detected in supernatants of iron-starved cells. spontaneous hemolysin-deficient mutants of one non-o1 strain were found to occur at high frequency. these variants also failed to synthesize vibrio ...	1988	2971620
the ultrastructure and atpase nature of polar membrane in campylobacter jejuni.	polar membrane in campylobacter jejuni has been visualized on membrane vesicles. it was composed of doughnut-shaped particles 5-6 nm in diameter, with stalks, arranged in a hexagonal array. this structure was stabilized on the membrane by a high ionic strength buffer in the presence of 2-mercaptoethanol. histochemical staining indicated localized atpase activity at the poles of the cells. an atpase with distinctive properties has been isolated and purified from this organism; it gives a specific ...	1988	2974756
atrial natriuretic factor alters autonomic interactions in the control of heart rate in conscious rats.	regulation of heart rate was studied in rats receiving either i.v. saline at 64 microl/min or synthetic 28-residue rat atrial natriuretic peptide (anf) at a dose sufficient to decrease mean arterial blood pressure by 10%. autonomic influences were deduced from steady-state heart rate responses of each group to propranolol, atropine, or propranolol and atropine combined. a multiplicative model of heart rate control was used to derive quantitatively from the data the modulation of intrinsic heart ...	1988	2975192
translational signals of a major head protein gene of bacteriophage lambda.	the d gene of bacteriophage lambda which codes for a major head protein is expressed at a high level during lytic growth. we have constructed a set of d-lacz gene fusions in order to examine the factors determining the high efficiency of the d translational initiation signals. it was found that an integral sequence, 300 bp long and upstream of the atg initiation codon, is required for maximal protein synthesis.	1988	2975351
[contamination of cheese with listeria monocytogenes].		1988	2975368
effects of ethanol withdrawal on beta-endorphin levels in rat brain and pituitary.	rats which received a liquid diet containing 6.5% (w/v) ethanol for three weeks became tolerant to the hypothermic effect of an acute dose of ethanol. withdrawal of this diet was followed by loss of the tolerance within 3 days, and by an accompanying pattern of changes in levels of immunoreactive-ir-beta-endorphin in several brain regions. an initial decrease in levels on days 1 and 3 of withdrawal was followed by recovery to control levels on days 8 and 15. this pattern was found in the arcuate ...	1988	2976169
pharmacological and behavioral effects of d1 dopamine antagonists.	this work includes the effects of sch 23390 and related benzazepines on behavior and attempts to relate these effects to their d1 dopamine antagonist action. effects on conditioned avoidance responding (car) in rats were studied under the same conditions in which in vivo binding of the radioiodinated d1 specific benzazepine 125i-sch 38840 was measured. it was found that there is very close agreement between the time-course for antagonism of car and for in vivo displacement of 125i-sch 38840 from ...	1988	2976247
the relationships between oligomeric structure and function of band 3 protein from human erythrocyte membranes: present knowledge and suggestions for further experiments.		1988	2976450
[ability of cholestyramine to bind escherichia coli and vibrio cholerae toxins].	cholestyramine treatment of new born's infectious diarrhea has been shown to be effective. the study of in vitro binding of bacterial toxins from vibrio cholerae and from three strains of escherichia coli suggest an ionic adsorption of the four toxins to this anion-exchange resin. this immediate binding is effective at the ph of intestinal fluid, therefore the protection of the enterocytes from the biological action of the toxins result of the toxins sequestering effect of the cholestyramine.	1988	3041907
correlations between gingival crevicular fluid enzymes and the subgingival microflora.	bacteroides gingivalis is a gram-negative micro-organism implicated in the pathogenesis of adult periodontitis and producing relatively large amounts of specific enzymes. in the present study, subgingival samples taken from adults with moderate periodontitis were examined for the presence and relative amounts of enzymatic activity toward certain substrates. enzyme levels were then correlated with clinical periodontal indices and microbiological analysis of subgingival plaque, including darkfield ...	1988	3042823
a glycolipid precursor of bacterial lipopolysaccharide (lipid x) lacks activity against endothelial cells in vitro and is not toxic in vivo.	lipid x (2,3-diacylglucosamine-1-phosphate) accumulates in mutants of escherichia coli incapable of assembling the disaccharide backbone of lipid a, the principle endotoxic moiety of bacterial lipopolysaccharide (lps). we compared the effects of lipid x on cultured bovine aortic endothelial cell (bec) viability and prostacyclin (pgi2) release with those of lipid a and lps. at 10(-5) m, both lps and lipid a produced significant bec cytotoxicity (percentage cytotoxicity 69 +/- 4 for lps and 51 +/- ...	1988	3043110
prostaglandins and the release of insulin. a review and a proposal.	the effect of prostaglandins or the inhibition of their synthesis on the release of insulin is controversial. dispute exists because there are apparently disparate experimental results. when the following factors are considered, however, much of the disparity is eliminated: 1) the experimental setting--in vitro or in vivo; 2) the experimental model--animal or human; 3) the experimental additive--the type of nonsteroidal antiinflammatory drug or specific prostaglandin; 4) the relationship of insu ...	1988	3043468
regulation of glucose homeostasis in rat jejunum by despentapeptide-insulin in vitro.	the regulation of the absorption and metabolism of glucose in rat small intestine by insulin was studied by the perfusion of isolated loops of proximal jejunum in vitro. the addition of an active, monomeric form of insulin, despentapeptide-insulin, to the serosal side of the intestine from normal rats inhibited luminal glucose absorption (421 (11) to 285 (11) mumol/h/g dry wt, p less than 0.001) and lactate production (340 (28) to 192 (26) mumol/h/g dry wt, p less than 0.001), but had no effect ...	1988	3044931
primary structure, synthesis, and biological activity of rat endothelin, an endothelium-derived vasoconstrictor peptide.	endothelin is a potent vasoconstrictor/pressor peptide, which we recently characterized from the conditioned culture medium of porcine aortic endothelial cells. we report here the cloning and partial sequencing of the rat endothelin gene. the nucleotide sequence predicted a 21-residue peptide similar to, but distinct from, porcine endothelin; 15 residues of rat endothelin were identical and 3 residues were substitutions by chemically similar amino acid residues to those in the porcine peptide. s ...	1988	3045827
history of discoveries of malaria parasites and of their life cycles.		1988	3045856
[use of quantitative immunofluorescence for determining the adsorption capacity of magnetic immunosorbents].	the adsorption capacity of microgranulated polyacrylamide magnetic immunosorbents has been studied by the method of quantitative immunofluorescence as applied to the causative agents of plague, cholera, and melioidosis. similar regularity in the dynamics of antigen adsorption on the granules of magnetic immunoadsorbents has been established. this regularity consists in the direct relationship between this process and the concentration of infective agents interacting with magnetic sorbents.	1988	3046196
[the protective properties of myelopeptides in the development of infectious processes caused by bacteria of the genus salmonella].	the protective properties of myelopeptides in the development of bacterial infection in mice and young pigs, caused by s. typhimurium 415, s. cholerae-suis 1422 and 370, have been studied. myelopeptides have been found to possess protective properties when injected into animals infected with s. typhimurium and s. cholerae-suis in lethal doses. the best protective effect (survival rate of 100%) has been achieved by the injection of myelopeptides 24 hours before challenge. myelopeptides have also ...	1988	3046197
melbourne trial of adjuvant immunotherapy in operable large bowel cancer.	a controlled randomized clinical trial was undertaken to assess the ability of combined non-specific and specific immunotherapy to alter the disease-free interval and overall survival of patients with stage b or c large bowel cancer. the immunotherapy consisted of a 2 year programme of vaccinations with bcg and neuraminidase-treated autologous tumour cells. three hundred and one patients entered the trial. at 5 years of follow-up there is no evidence that this form of immunotherapy can alter eit ...	1988	3046593
[the role of the rs1 sequence of vibrio cholerae in the amplification of a segment of plasmid dna carrying the tetracycline resistance gene and cholera toxin genes].	the clones with 4 to 30-fold increased level of tetracycline resistance (tcr) were selected from the strain of escherichia coli k-12 carrying the pco107 plasmid. the plasmid is the cointegrate formed from the plasmids pox38 (f-derivative) and pct105 (pbr322-derivative carrying the vct operon of vibrio cholerae eltor and the rsi sequence). pco107 contains rsi at the junctions of two plasmid genomes. using restriction analysis and southern blot hybridization technique it was shown that increased t ...	1988	3047003
the nucleotide sequence and gene organization of red clover necrotic mosaic virus rna-2.	red clover necrotic mosaic virus, a member of the dianthovirus group, is characterized by a genome composed of two nonhomologous single-stranded rnas of approximately 4.0 (rna-1) and 1.4 kb (rna-2). the complete nucleotide sequence of the rna-2 has been determined. rna-2 is 1448 nucleotides in length with a 5' terminal m7g cap and no 3' terminal poly-a tail or 5' terminal vpg. an open reading frame beginning at the first initiation codon at nucleotide 80 and ending at nucleotide 1030 has been id ...	1988	3047682
porcine muscle prolyl endopeptidase: limited proteolysis of tryptic peptides from hemoglobin beta-chains at prolyl and alanyl bonds.	tryptic peptides from hemoglobin (hb) beta-chains were used as model substrates for limited proteolysis by prolyl endopeptidase (ec 3.4.21.26) from porcine muscle. from the physicochemical and enzymatic properties of prolyl endopeptidase the conditions for routine digestion were established as follows: the molar ratio of enzyme to substrate was 1 to 100, and the reaction was carried out in sodium phosphate buffer (ph 6.4) at 37 degrees c for 4 h. under these conditions the peptide bonds on the c ...	1988	3048412
porcine muscle prolyl endopeptidase: limited proteolysis of tryptic peptides from hemoglobin beta-chains at prolyl and alanyl bonds.	tryptic peptides from hemoglobin (hb) beta-chains were used as model substrates for limited proteolysis by prolyl endopeptidase (ec 3.4.21.26) from porcine muscle. from the physicochemical and enzymatic properties of prolyl endopeptidase the conditions for routine digestion were established as follows: the molar ratio of enzyme to substrate was 1 to 100, and the reaction was carried out in sodium phosphate buffer (ph 6.4) at 37 degrees c for 4 h. under these conditions the peptide bonds on the c ...	1988	3048412
national institutes of health consensus statement. health implications of smokeless tobacco use. nih consensus development panel.	national data indicate that at least 10 million persons have used smokeless tobacco within the past year. the human evidence that use of snuff causes cancer of the mouth is strong. smokeless tobacco use increases the frequency of localized gum recession and leukoplakia where the snuff is usually placed. the presence of lead in smokeless tobacco may pose a special risk for the developing fetus. use of smokeless tobacco releases nicotine into the bloodstream and produces blood levels of nicotine c ...	1988	3048429
abdominal cryptococcoma in aids: a case report.	cryptococcosis is a recognized opportunistic pathogen in the acquired immune deficiency syndrome. although central nervous system infection and disseminated cryptococcosis is common in acquired immune deficiency syndrome, localized infection is rare. we present a case of massive retroperitoneal and mesenteric adenopathy in a male homosexual patient with acquired immune deficiency syndrome with clinical and radiologic features suggestive of lymphoma. however, this was proven pathologically to rep ...	1988	3048904
the role of heterochromatin in the origin of isochromosome 1 in neoplastic cells.		1988	3049113
extracellular proteins of vibrio cholerae: nucleotide sequence of the structural gene (hlya) for the haemolysin of the haemolytic el tor strain 017 and characterization of the hlya mutation in the non-haemolytic classical strain 569b.	the ei t or haemolysin, product of hlya, is exported from vibrio cholerae as a mr 80,000 protein which can be subsequently cleaved to give two proteins of mr 65,000 and 15,000. nucleotide sequence analysis has demonstrated that hlya encodes a protein of mr 82,250 with a potential 18-amino-acid signal sequence. the non-haemolytic classical strain 569b has been shown to have a structural gene defect rather than a defect in secretion. by non-reciprocal recombination it was possible to transfer this ...	1988	3050359
production and partial purification of a fluid-accumulating factor of non-o1 vibrio cholerae.	a fluid-accumulating factor (faf in the ligated rabbit ileal loop test) from a strain of non-o1 vibrio cholerae not producing cholera toxin-like enterotoxin (ctlt) was partially purified by ammonium sulfate precipitation, gel filtration with sephadex g-100, and deae cellulose column chromatography. the preparation thus obtained showed collagenolytic, cytolytic, necrotic, and hemorrhagic activities, but was not lethal to mice nor hemolytic to sheep erythrocytes. desquamation of epithelial cells, ...	1988	3050379
[pro-allergy role of infection. a component of the mode of reacting].	no organism from the cradle lives germ-free or allergen-free. the modalities by which infections facilitate conditions of abnormal reactivity, in particular respiratory asthma and allergy to cow milk proteins, are examined. 216 asthmatic children and 50 infants with rotavirus enteritis have been considered. infections, besides representing stimuli directly projected on the immune system, also constitute factors which more generally influence the way of reacting.	1988	3050902
[instability of immunoglobulin expression during differentiation induction in the human lymphoblastoid cell line rpmi-6410t].	the induction of immunoglobulin heavy chain classes switch from igm to igg was demonstrated in vitro in cells of rpmi-6410t line. the igg+-sublines, formed as a result of the switch are characterized by instability of igg synthesis. after removal of the inductor from the growth environment, igg+ cells gradually reduce the level of secreted igg. such a transition to the functional rest state is likely to be connected with the convertible ig-gene activity suppression in igg+ cells, since after the ...	1988	3051603
[bacteriologic characteristics of 25 strains of vibrio cholerae, biotype eltor isolated in mali].	this work studies bacteriological characters of 25 strains of v. cholerae isolated in mali during the outbreak of 1984-1987. these strains were precisely identified by biochemistry characters, toxin secretion, presence of a structure considered as attachment factor. antibiotic susceptibility study confirmed the great sensibility of v. cholerae to this products, and allowed to classify them according to their cl90. this contribution of the laboratory is very interesting for the control of the dis ...	1988	3052895
[2 hemocultures positive for vibrio cholerae].	the authors report two cases of vibrio cholerae isolation in hemocultures even though the copro-cultures where negative. in both cases, post-operated patients are concerned. the first one had supported a renal transplantation and was under immuno-depressor. the second one, after an aorto-aneurysm surgical treatment suffered from a mesenteric infarct. during the disease the vibrio was found in the hemoculture.	1988	3052896
detection of vibrio cholerae with monoclonal antibodies specific for serovar o1 lipopolysaccharide.	six hybridoma cell lines, each of which produced a monoclonal antibody (mab) against vibrio cholerae o1 lipopolysaccharide (lps), were established. each mab was active serologically by both enzyme-linked immunosorbent assay (elisa) and the slide agglutination test. in the elisa, each mab was tested against 7 o1 and 9 non-o1 lps preparations. three mabs reacted with both inaba and ogawa serovars (a antigen), two mabs reacted with the ogawa serovars only (b antigen), and one mab reacted with the i ...	1988	3053778
motility of campylobacter jejuni in a viscous environment: comparison with conventional rod-shaped bacteria.	the motility of four strains of campylobacter jejuni in solutions of varying viscosity was measured and compared to that of a number of conventional rod-shaped bacteria (crsb). all the bacteria tested showed an initial increase in velocity in the low viscosity solutions--between 1 and 3 centipoise (1 p = 0.1 pa s). however, only the campylobacters were actively motile in highly viscous solutions with velocities ranging from 60 to 100 micron s-1. all strains of c. jejuni tested showed three separ ...	1988	3053972
accumulation of lamb-lacz hybrid proteins in intracytoplasmic membrane-like structures in escherichia coli k12.	the subcellular location of lamb-lacz hybrid proteins in the escherichia coli k12 strains pop3234 and pop3299 was investigated by immunocytochemical detection and protease-accessibility experiments. induction of the synthesis of the hybrid proteins resulted in the appearance of membrane-like structures within the cytoplasm of the cells. labelling of ultrathin cryosections of the cells with anti-beta-galactosidase or anti-lamb protein serum and protein-a-gold complexes revealed that the hybrid pr ...	1988	3053974
[reversible suppression and activation of immunoglobulin genes in differentiated igg+ sublines of human rpmi-6410t b-cells].	differentiated human b-cells of the igg+ sublines obtained as a result of switching from igm to igg synthesis in the 6410t line and its igm+ lines gradually reduce the level of igm secretion after the inductor removal. igg synthesis can be partially or fully recovered by treating the igg+ sublines with a polyclonal activator of b-lymphocytes, lipopolysaccharide w from gram negative bacteria. in the conditions of certain regulatory effects, differentiated igg+ cells are capable to pass reversibly ...	1988	3054675
non-o:1 vibrio cholerae bacteremia: case report and review.	a case of non-o:1 vibrio cholerae bacteremia and prostatic abscess in a patient with idiopathic aplastic anemia was studied, and the data were compared with those from 23 previously reported cases of non-o:1 v. cholerae bacteremia. the case-fatality rate for the 13 cases for which the outcome is known is 61.5%. the majority of known cases have occurred in immunocompromised patients, particularly those with hematologic malignancy or cirrhosis. host susceptibility is potentially important in this ...	1988	3055182
[morphologic characterization of the pathogenic action of cholera and nonagglutinating vibrios].		1988	3055769
[the vesalius of human embryology].	human embryology was founded 100 years ago by wilhelm his, who may rightly be called the "vesalius of human embryology". 3 landmarks were published in germany within a quarter of a century: the anatomie menschlicher embryonen of his, keibel's normentafeln, and keibel and mall's handbuch der entwicklungsgeschichte des menschen. the staging of the human embryo, now associated with the names of streeter and o'rahilly, was first proposed by mall in anatomischer anzeiger.	1988	3056108
in vivo localization and biological effect of anti-yolk sac monoclonal antibodies.	two monoclonal antibodies directed against rat yolk sac antigen 1 (mab 6d1) and yolk sac antigen 2 (mab 3c3) were injected i.v. into pregnant or tumor-bearing rats. immunofluorescent examination of the different organs from animals killed, 2, 24, or 48 hours after inoculation showed the specific binding of mab 6d1 to the free surface of visceral endoderm cells in pregnant animals and on visceral cells of yolk sac carcinoma. the mab 3c3 reacted only with the endoderm of parietal yolk sac and with ...	1988	3056111
[molecular basis of human hereditary diseases caused by gene expression deficiency].	the recent literature on the structure of mutant human genes determining the phenotypic expression of hereditary diseases is reviewed. the main types of gene mutations are described including deletions and insertions, mutations impairing translation and posttranslational protein processing, m-rna processing, causing transcription blocks, ultimate or partial quantitative deficiency in proteins-gene products. the problems of genetic heterogeneity of hereditary diseases and diagnosis of the latter ...	1988	3057356
[molecular basis of human hereditary diseases caused by gene expression deficiency].	the recent literature on the structure of mutant human genes determining the phenotypic expression of hereditary diseases is reviewed. the main types of gene mutations are described including deletions and insertions, mutations impairing translation and posttranslational protein processing, m-rna processing, causing transcription blocks, ultimate or partial quantitative deficiency in proteins-gene products. the problems of genetic heterogeneity of hereditary diseases and diagnosis of the latter ...	1988	3057356
a mutant human proinsulin is secreted from islets of langerhans in increased amounts via an unregulated pathway.	a coding mutation in the human insulin gene (his-b10----asp) is associated with familial hyperproinsulinemia. to model this syndrome, we have produced transgenic mice that express high levels of the mutant prohormone in their islets of langerhans. strain 24-6 mice, containing about 100 copies of the mutant gene, were normoglycemic but had marked increases of serum human proinsulin immunoreactive components. biosynthetic studies on isolated islets revealed that approximately 65% of the proinsulin ...	1988	3057496
a mutant human proinsulin is secreted from islets of langerhans in increased amounts via an unregulated pathway.	a coding mutation in the human insulin gene (his-b10----asp) is associated with familial hyperproinsulinemia. to model this syndrome, we have produced transgenic mice that express high levels of the mutant prohormone in their islets of langerhans. strain 24-6 mice, containing about 100 copies of the mutant gene, were normoglycemic but had marked increases of serum human proinsulin immunoreactive components. biosynthetic studies on isolated islets revealed that approximately 65% of the proinsulin ...	1988	3057496
current perspectives on the epidemiology and pathogenesis of clinically significant vibrio spp.	recent taxonomic advances have now implicated several different vibrio species as human pathogens. while the most common clinical presentation of vibrio infection continues to be gastroenteritis, an increasing number of extraintestinal infections are being reported, particularly in immunocompromised individuals. detection of vibrio infections requires a good clinical history and the use of appropriate isolation and identification procedures by the laboratory to confirm illnesses attributed to vi ...	1988	3058295
factors affecting the survival of pathogenic bacteria in subtropical river water.	the present study was carried out to determine the factors causing the disappearance of introduced salmonella enteritidis, staphylococcus aureus, and vibrio cholerae in natural river water. when large numbers of the above organisms (final level 10(7)-10(8) cfu per ml) were added to river water, the decline of their numbers coincided with increasing numbers of protozoa. furthermore, their survival was improved dramatically if the river water was amended with a eucaryote inhibitor. after comparing ...	1988	3058406
genetic fusion of a non-toxic heat-stable enterotoxin-related decapeptide antigen to cholera toxin b-subunit.	a decapeptide highly homologous to the sta escherichia coli heat-stable enterotoxin and to several other heat-stable enterotoxins was fused genetically to the amino-end of the b-subunit of cholera toxin (ctb) and the hybrid protein gene expressed from a tacp overexpression system. the sta-related decapeptide used, which was encoded by a synthetic oligodeoxynucleotide, contained a single mutation which substituted a disulfide-linked cysteine by alanine. after its fusion to ctb the decapeptide was ...	1988	3058509
an epidemic of multiresistant salmonella typhimurium with a characteristic plasmid profile among calves in tokachi district, japan.		1988	3058996
identification and localization of a novel, cytoskeletal, centrosome-associated protein in ptk2 cells.	antisera raised against centrin (salisbury, j.l., a.t. baron, b. surek, and m. melkonian. 1984. j. cell biol. 99:962-970) have been used, here, to identify a centrosome-associated protein with an mr of 165,000. immunocytochemistry indicates that this protein is a component of pericentriolar satellites, basal feet, and pericentriolar matrix of interphase cells. these components of pericentriolar material are, in part, composed of 3-8-nm-diam filaments, which interconnect to form a three-dimension ...	1988	3060471
effect of dexamethasone on experimental escherichia coli mastitis in the cow.	the effect of intramuscular administration of dexamethasone on milk production and on several clinical and hematologic indicators in cows with experimental escherichia coli mastitis was studied. two groups of three cows each were intramammarily inoculated with 20 x 10(5) cfu e. coli 0101 k99 f41 in rear quarters. immediately after inoculation one groups received 30 mg dexamethasone intramuscularly, whereas the other group was injected similarly with pyrogen-free saline. fourteen days later milk ...	1988	3060491
occupational health issues for providers of care to patients with hiv infection.	the perception that hiv is a highly contagious nosocomial pathogen persists despite a great deal of evidence to the contrary. however, health care workers are at a low but finite risk of occupational infection with the virus, particularly through accidental needle-stick inoculations with infected blood. universal blood and body fluid precautions minimize the risk of nosocomial transmission of a variety of pathogens, including hiv, and should be routinely employed by all health care workers for a ...	1988	3060519
fungal infections in aids. cryptococcosis.	cryptococcus neoformans, histoplasma capsulatum, and coccidioides immitis are the three fungi that regularly cause disseminated, life-threatening disease in patients with aids. cryptococcosis is the fourth most common opportunistic infection in patients with aids and results in meningitis or pneumonia or both, in most cases. in addition, there have been unusual focal infections described and even unexplained fever alone. in any patient at risk for hiv infection, routine screening tests should in ...	1988	3060529
[pathogenicity factors of vibrio cholerae].		1988	3061255
[characteristics of the protease activity of vibrio cholerae strains and mutants with various biological properties].	the study of 65 v. cholerae strains and mutants revealed that protease activity was high in choleragenic strains and mostly low, sometimes even not manifested, in noncholeragenic strains. the use of inhibitors made it possible to determine three types of protease. choleragenic strains were found to possess all three types of protease, while in noncholeragenic strains only protease of type 1 was detected in very rare cases. in noncholeragenic hemolytic strains and mutants protease of type 3 and i ...	1988	3061263
isolation and chemical analysis of 7-o-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-heptose as a constituent of the lipopolysaccharides of the udp-galactose epimerase-less mutant j-5 of escherichia coli and vibrio cholerae.	methyl 7-o-(2-amino-2-deoxy-alpha-d-glucopyranosyl)-l-glycero-d-manno-hepto+ ++ pyranoside (1) was released from the lipopolysaccharide of the udp-galactose epimerase-less mutant j-5 of escherichia coli by methanolysis and isolated by high-voltage paper electrophoresis. its chemical structure was determined by chemical analysis, deamination with nitrous acid, g.1.c.-m.s., and 1h- and 13c-n.m.r. spectroscopy performed on its acetylated derivative. the disaccharide moiety of 1 was also detected in ...	1988	3061645
antipeptide monoclonal antibodies inhibit the binding of rabies virus glycoprotein and alpha-bungarotoxin to the nicotinic acetylcholine receptor.	it has been reported that binding to muscle nicotinic acetylcholine receptor at the post-synaptic membrane is an important event of the rabies virus neurotropism. the binding site can be located within the 190-203 region of the virus glycoprotein sharing a high degree of homology with the "toxic loop" of the curare-mimetic snake neurotoxins. we have synthesized a tetradecapeptide corresponding to this glycoprotein region and used it, following conjugation with an immunogenic carrier to raise mab ...	1988	3062388
the appearance of transferrin receptors on cultured human cytotrophoblast and in vitro-formed syncytiotrophoblast.	using trypsinization and percoll gradient centrifugation, mononuclear cell populations were prepared from human term placentae. these populations consist mainly of cytotrophoblast, which probably lacks transferrin receptors. in vitro, transferrin receptors appear on these mononuclear cells and on syncytiotrophoblast formed in vitro, independently, of cellular fusion and proliferative state.	1988	3062615
mode of action of morpholine derivatives.		1988	3063169
glucose inhibits prostacyclin production by cultured aortic endothelial cells.	a reduction in production of prostacyclin (pgi2) by the cells in the vascular wall may play a role in the pathogenesis of atherosclerosis in diabetic patients. the present study was undertaken to evaluate the effect of glucose on pgi2 production by endothelial cells in vitro. it was shown that pgi2 production by cultured bovine aortic endothelial cells was significantly reduced in the presence of a high concentration of glucose (300 mg/dl) compared with physiological concentrations of glucose (1 ...	1988	3064357
clinical pharmacokinetics of anthelmintic drugs.	a rational strategy for chemotherapy demands that dosage schedules be based on an adequate knowledge of clinical and biochemical pharmacology. many anthelmintic drugs (e.g. suramin, diethylcarbamazine, hycanthone) were introduced before modern techniques for drug evaluation (controlled clinical trials) and before the development of specific and sensitive analytical methods for the assay of drugs and metabolites in biological fluids. thus, many of the regimens used today for the treatment of para ...	1988	3064952
alterations in the chemical composition and antigenicity of escherichia coli o89 lipopolysaccharide and lipid a after 60co gamma irradiation.	the chemical composition and the antigenicity of escherichia coli lipopolysaccharide (lps) and lipid a were investigated after irradiation with 150 kgy 60co-gamma ray. compared to the original preparations, the irradiated lps showed a significant reduction in glucosamine, glucose and galactose and a decrease in the phosphate content. the fatty acid components were reduced to a smaller degree. irradiation induced a reduction in the antigenicity of the polysaccharide part. the amount of glucosamin ...	1988	3067502
alterations in the chemical composition and antigenicity of escherichia coli o89 lipopolysaccharide and lipid a after 60co gamma irradiation.	the chemical composition and the antigenicity of escherichia coli lipopolysaccharide (lps) and lipid a were investigated after irradiation with 150 kgy 60co-gamma ray. compared to the original preparations, the irradiated lps showed a significant reduction in glucosamine, glucose and galactose and a decrease in the phosphate content. the fatty acid components were reduced to a smaller degree. irradiation induced a reduction in the antigenicity of the polysaccharide part. the amount of glucosamin ...	1988	3067502
epidemiological patterns and incidence of bio-, sero- and phage types of vibrio cholerae in hyderabad, india, during 1971-1984.	during 1971-1984 out of 44,762 gastroenteritis cases bacteriologically examined, 4240 (9.5%) vibrio cholerae were isolated in hyderabad, india. out of them 1329 (31.3%) were classical and 2911 (68.7%) were el-tor biotypes. the changeover from classical to el-tor cholera was observed in hyderabad during 1975 and persisted. during this 14 year period four major outbreaks with v. cholerae serogroup o1 ogawa, v. cholerae biotype el-tor serogroup o1 ogawa were observed. phage types 1, 2 and 4 were pr ...	1988	3067503
somatomedin c immunoreactivity in the achilles tendon varies in a dynamic manner with the mechanical load.	distribution of the trophic peptide somatomedin c (sm-c; insulin-like growth factor i; igf-i) immunoreactivity was mapped in normal achilles and tibialis anterior tendons. the spindle-shaped tendon fibroblasts showed faint perinuclear staining. fibroblasts in the paratenon mostly had a more intense igf-i immunoreactivity, i.e. faint to moderate. when analysing either tendon in detail, areas with more intense igf-i immunoreactivity could be recognized and seemed to correlate with areas of high me ...	1988	3067520
somatomedin c immunoreactivity in the achilles tendon varies in a dynamic manner with the mechanical load.	distribution of the trophic peptide somatomedin c (sm-c; insulin-like growth factor i; igf-i) immunoreactivity was mapped in normal achilles and tibialis anterior tendons. the spindle-shaped tendon fibroblasts showed faint perinuclear staining. fibroblasts in the paratenon mostly had a more intense igf-i immunoreactivity, i.e. faint to moderate. when analysing either tendon in detail, areas with more intense igf-i immunoreactivity could be recognized and seemed to correlate with areas of high me ...	1988	3067520
physiological and behavioral aspects of stress in adolescence.	gonadal and adrenal hormone levels appear to be linked to the stressors experienced by young adolescents. adjustment problems were accompanied by a profile of lower gonadal steroids and higher adrenal androgen levels, primarily androstenedione. later gonadal maturation may be a result of stress suppressing the reproductive axis. higher levels of androstenedione may be indicative of chronic levels of stress. however, the findings for androstenedione are complicated by the fact that androstenedion ...	1988	3067560
group b streptococcal infections. perinatal impact and prevention methods.		1988	3067638
kluyvera species soft tissue infection: case report and review.	a soft tissue infection caused by kluyvera species in a previously healthy woman is described. successful treatment required incision and drainage of the wound in addition to administration of antibiotics. kluyvera species are indole positive, voges-proskauer negative members of the family enterobacteriaceae. previous reports of infection due to kluyvera species suggest that the organism is never more than an opportunistic pathogen; however, we report the first case of infection in a previously ...	1988	3068255
stimulation of escherichia coli division by low-intensity monochromatic visible light.		1988	3068688
management and prevention of bacterial diarrhea.	the etiology, pathogenesis, transmission and communicability, diagnosis, and management of infectious diarrhea are reviewed. infectious diarrhea is common in both industrialized and unindustrialized areas of the world. better understanding of the etiology and pathogenesis and identification of "new" enteric pathogens has resulted in the use of a variety of drugs to relieve symptoms or to effect a clinical cure. both host and microbial virulence factors are key in the acquisition of diarrheal ill ...	1988	3069286
cloned polynucleotide and synthetic oligonucleotide probes used in colony hybridization are equally efficient in the identification of enterotoxigenic escherichia coli.	restriction endonuclease-generated polynucleotide and synthetically produced oligonucleotide gene probes used in colony hybridization assays proved to be efficient for the detection and differentiation of enterotoxigenic escherichia coli. to compare their relative efficiencies, these two sets of probes were radiolabeled with 32p and were applied to 74 strains of e. coli with known enterotoxin profiles and to 156 previously unexamined e. coli isolates. the enterotoxigenic bacteria vibrio cholerae ...	1988	3069860
[vaccination against cholera: what prospects?].		1988	3071278
effect of interleukin-1 on the biosynthesis of proinsulin and insulin in isolated rat pancreatic islets.	insulin dependent diabetes mellitus (iddm) is often preceded or associated with lymphocytic infiltration in the islets of langerhans (insulitis). we recently demonstrated that interleukin-1 (il-1) produced by activated macrophages exerts a bimodal effect on insulin release and biosynthesis in isolated rat islets. in the present study we have further analysed the effect of recombinant human interleukin-1 beta (ril-1) on the biosynthesis and conversion of proinsulin 1 and 2 in rat islets. by rp-hp ...	1988	3071362
[ultrastructural changes induced by antiseptics in various bacterial species].	due to the succinate-neotetrazolium technique, the authors were able to photograph with electronic microscopy, for the first time, different antiseptics, including "javelle water", attacking bacteria.	1988	3071430