Publications

effect of doxorubicin on mouse hybridoma b cells: stimulation of immunoglobulin synthesis and secretion.	the purpose of these studies was to investigate whether the cell-differentiating effect of anthracyclines can trigger an over-production and secretion of molecules that may interfere with the tumor-host relationship. we exposed mouse hybridoma b-cells, which are devoted to immunoglobulin production, to doxorubicin (10-40 ng/ml). we found that most doxorubicin-treated cells secreted 3- to 5-fold higher amounts of immunoglobulin than untreated cells, along with an accumulation of 50% of them in th ...	1989	2788496
a t-cell-specific transcriptional enhancer element 3' of c alpha in the human t-cell receptor alpha locus.	a transcriptional enhancer element has been identified 4.5 kilobases 3' of c alpha (constant region alpha chain) in the human t-cell receptor (tcr) alpha-chain locus. this enhancer is active on both a tcr v alpha (variable region alpha chain) promoter and the minimal simian virus 40 promotor in tcr alpha/beta jurkat and el4 cells but is inactive on a v alpha promoter in human tcr gamma/delta peer and molt-13 cells, clone 13 b cells, and hela fibroblasts. the enhancer has been localized to a 116- ...	1989	2788889
effect of vitamin d metabolites on calcitriol metabolism in experimental renal failure.	previous studies from our laboratory have demonstrated that metabolic clearance rate (mcr) of calcitriol is decreased in experimental renal failure. in this experiment, we examined the effects of calcitriol, 25-hydroxyvitamin d3 (25(oh)d3) and 24,25-dihydroxyvitamin d3 (24,25(oh)2d3) on the mcr of calcitriol in renal failure produced in rats by partial nephrectomy. the mcr of calcitriol in these rats with renal failure was significantly lower than in control rats with sham operations. plasma con ...	1989	2789310
potentiation by vestibular stimulation of the immobility reflex elicited by clamping in developing and adult rats.	we have previously described (exp. neurol., 97: 315-326, 1987) that clamping of the neck elicits a profound immobility with flexion of limbs and ventroflexion of head and neck ('carrying posture'). we also observed that when a clamped rat pup is carried by the experimenter's hand, such carrying posture was potentiated. in the present paper we observed that in adult rats vestibular stimulation by circular acceleration potentiates the duration of immobility reflex by clamping and intensifies the l ...	1989	2789702
squamous cell carcinoma of the base of the tongue: results of treatment in 115 cases.	between 1976 and 1986, we treated 115 patients (mean age 53.8 years) with base of tongue carcinomas. the staging system used was the uicc tnm classification of 1979. seventy per cent of the tumours were t3 or t4 and 42% had n2 or n3 lymph node. locoregional treatment was irradiation alone (98/115) or surgery and post-operative radiotherapy (17/115). sixty-seven patients received induction chemotherapy. actuarial survival of the entire group at 3 and 5 years was 25 and 23%, respectively, and 3-ye ...	1989	2790426
[clinical and experimental research on a kidney-tonifying prescription in preventing and treating children's hearing-loss induced by aminoglycoside antibiotic ototoxicity].	this paper reports 30 cases of hearing-loss induced by aminoglycoside antibiotic ototoxicity treated mainly with kidney-tonifying herbs such as herba epimedii, rhizoma drynariae, rhizoma polygonati, radix polygoni multiflori, magnetitum and rhizoma acori graminei, etc. after three month's treatment, the authors found that 19 cases had improved their hearing more than 15 db, among which there are 8 cases who had improved 15 db and 11 cases 30 db. the patients' hearing of pre-treatment and post-tr ...	1989	2791158
relative contribution of various forms of cytochrome p450 to the metabolism of benzo[a]pyrene by human liver microsomes.	a variety of cytochromes p450 have been implicated in the hepatic metabolism of benzo[a]pyrene (bp), including forms that are constitutively expressed and those that are highly inducible. in the present study the metabolism of bp to organic solvent-soluble derivatives by eight forms of cytochrome p450 isolated from rat liver and by a series of 11 human liver microsomal samples was investigated. the relative contribution of specific p450 forms to the human hepatic metabolism was evaluated. a 4-fo ...	1989	2791199
effect of platelet factors on migration of cultured bovine aortic endothelial and smooth muscle cells.	endothelial cell (ec) injury and the response of ec and smooth muscle cells (smcs) to injury contribute to the pathophysiology in patients with vascular disease and atherosclerosis. since platelets have been suggested to play an important role in modulating vascular injury, the present study was undertaken to examine the influence and mechanism of action of individual platelet factors on bovine aortic ec and smc migration using an in vitro wound assay system. serotonin decreased ec proliferation ...	1989	2791219
intensive care for patients with pneumocystis carinii pneumonia and respiratory failure. are we prepared for our new success?		1989	2791661
[ferric ammonium citrate as virulence-enhancing agent in the toxicity test of vibrio cholerae and the potency assay of cholera vaccine].	using icr strain mice as experimental animals, 0.25% (w/v) ferric ammonium citrate (fac) was a suitable substitute for 5% (w/v) mucin as a virulence-enhancing (ve) agent in the toxicity test of vibrio cholerae and the potency assay of cholera vaccine. there was no significant lethal toxicity difference between 0.25% fac and 5% mucin as ve agents on vibrio cholerae in mice. by using 0.25% fac as a ve agent in estimating the potency of cholera vaccine, the relative potency (r.p.) of the tested cho ...	1989	2791724
visual evoked potentials in prader-willi syndrome.	oculocutaneous, electrophysiological, and cytogenetic factors were evaluated in 14 patients with prader-willi syndrome and in three controls, two albinos and a normal observer. in a substantial number of pw patients chromosomal anomalies, particularly deletions of the long arm of chromosome 15 as well as hypopigmentation of hair, skin, and eye have been identified. in the genetic condition of albinism, hypopigmentation related to neural ectoderm derivatives is associated with reduced visual acui ...	1989	2791841
interaction between prostatic fibroblast and epithelial cells in culture: role of androgen.	we have established and characterized two cell lines from the ventral prostate gland of normal nb rats: nbe-1 (prostatic epithelial) and nbf-1 (prostatic fibroblast) cell lines. to identify the direct mitogenic action of dihydrotestosterone (dht), we incubated these cell lines alone and together (in the presence and absence of cell contact) with various concentrations of dht (0.1-10,000 ng/ml) for 24-72 h and assayed for the rate of dna synthesis and the total number of cells in tissue culture a ...	1989	2792005
bolus injections of recombinant human interleukin-1 cause transient hypocalcemia in normal mice.	single daily injections of recombinant human interleukin-1 alpha (il-1 alpha) or interleukin-1 beta (il-1 beta) were given for 1-3 days to normal mice, and blood ionized calcium concentrations were measured before and at various times after each injection. mean blood calcium levels fell significantly in both groups of animals 3 h after the first il-1 injections and returned to pretreatment values at 5 h. however, at 24 and 48 h mean values were significantly higher than those in saline-heated co ...	1989	2792008
mechanisms of arterial thrombosis in nonparallel streamlines: platelet thrombi grow on the apex of stenotic severely injured vessel wall. experimental study in the pig model.	the role of thrombosis in various acute coronary syndromes has been established. however, the basic mechanism by which plaque rupture leads to a growing thrombus in the vicinity of stenotic lesions is not well understood. using a characterized flow chamber in a rheologically controlled system, we have mimicked stenotic vessels and studied for the first time cell-vessel wall interaction in nonparallel streamlines. stenoses ranging from 0 to 80% were produced with stripped tunica media to mimic se ...	1989	2794050
a case of non-o:1 vibrio cholerae bacteremia from northern europe.		1989	2794570
mutation induced by vibriophage ps166 infection changes biotype and phage type of vibrio cholerae.	the effect of a newly isolated vibriophage, ps166, on vibrio cholerae (e1 tor) mak757 was investigated. two ps166-resistant mutants of strain mak757 were isolated. these had undergone transition to the classical biotype with concomitant acquisition of new phage sensitivity. however, the parental ogawa serotype remained unchanged. these mutant strains also showed a unique temperate phage-sensitivity profile, distinct from that of strain mak757. the possible target of phage ps166 interaction is di ...	1989	2795637
[monoclonal antibodies to antigens of cholera vibrios of the ogava serovar].	a set of hybrids is obtained synthesizing monoclonal antibodies to the surface antigenic determinants of the choleric vibrio of the ogava serovar. the antigenic structure of vibrios of the typical and atypical strains isolated from a man and from environmental objects is studied using a collection of highly specific immunoglobulins. the high-specific diagnostic preparations for identification of the 01-group cholera agent serovar may be created on their base.	1989	2796789
the activity of phosphoenolpyruvate carboxykinase throughout the lactation cycle of the guinea pig mammary gland.	the enzyme phosphoenolpyruvate carboxykinase (pepck) has been measured in the guinea pig mammary gland throughout the pregnancy-lactation cycle. this is of interest since the primary importance of pepck is thought to be its role in gluconeogenesis and it is questionable whether or not gluconeogenesis occurs in the mammary gland. the enzyme activity, present in both the cytosol and mitochondria, was shown to follow the lactation profile. during the transition into lactation, cytosolic pepck activ ...	1989	2798434
the activity of phosphoenolpyruvate carboxykinase throughout the lactation cycle of the guinea pig mammary gland.	the enzyme phosphoenolpyruvate carboxykinase (pepck) has been measured in the guinea pig mammary gland throughout the pregnancy-lactation cycle. this is of interest since the primary importance of pepck is thought to be its role in gluconeogenesis and it is questionable whether or not gluconeogenesis occurs in the mammary gland. the enzyme activity, present in both the cytosol and mitochondria, was shown to follow the lactation profile. during the transition into lactation, cytosolic pepck activ ...	1989	2798434
[tuberculosis of the lymph nodes and spleen preceding hodgkin's disease].	tuberculosis of the lymph nodes is not rare today. however tuberculosis of the spleen has been very rare even in the past when many patients had suffered from tuberculosis. the appearance of hodgkin's disease following tuberculosis is an extremely rare condition. it has thought that deficiency of cellular immunity was responsible for both tuberculosis and malignancy. we present a 44-year-old man in whom lymph node and spleen tuberculosis antedated the clinical onset of hodgkin's disease for thre ...	1989	2799551
survival of vibrio cholerae in african domestic water storage containers.	although much has been published about growth of vibrio cholerae in natural sources of water, little or nothing has been written on its survival in common household water storage containers. this study compared the behaviour of the el tor and classical biotypes of v. cholerae in different containers commonly used in africa for domestic water storage. these included traditional clay pots, wooden barrels, galvanised iron drums (corroded and non-corroded) and plastic (polyethylene) drums. the findi ...	1989	2799584
hemagglutination and intestinal adherence properties of clinical and environmental isolates of non-o1 vibrio cholerae.	hemagglutination and intestinal adherence properties of non-o1 vibrio cholerae were studied in vitro. no definite correlation between the cell-associated hemagglutinin titers and the intestinal adhesion indices was noted. sugar- and glycoprotein-mediated inhibition data also indicated differences between the hemagglutination and adherence processes in respect to the receptor structures. intestinal adherence of most v. cholerae strains could be inhibited to various extents by n-acetyl d-glucosami ...	1989	2802613
a case report of acute obstructive suppurative cholangitis in a non-0-1 vibrio cholerae biliary carrier.	a case report is presented of a 73-year-old male who was seen with fever, jaundice, abdominal pain and central nervous system depression. he failed to respond to intensive antibiotic therapy, and subsequently acute obstructive suppurative cholangitis fully developed. upon laparotomy, the patient's gallbladder was found to be enlarged with the bile from the gallbladder and bile duct itself containing a high pus content. its cultured organism revealed non-0-1 vibrio cholerae. to our knowledge, no ...	1989	2802912
[studies on tissue distribution of unusual estrogen-binding protein in rats with the help of immunoblotting].	tissue distribution in male and female rats of unusual immunoreactive material specific for the hepatic estrogen-binding protein (uebp) was studied by means of immunoblotting analysis. from 28 organs and tissues studied only the liver of animals of both sexes was found to contain polypeptide with mr, close to mr of purified uebp (approximately 31,000), which reacts with anti-uebp specific antiserum. detection of the immunoreactive material in female liver, which does not contain any hormone-bind ...	1989	2804329
[studies on tissue distribution of unusual estrogen-binding protein in rats with the help of immunoblotting].	tissue distribution in male and female rats of unusual immunoreactive material specific for the hepatic estrogen-binding protein (uebp) was studied by means of immunoblotting analysis. from 28 organs and tissues studied only the liver of animals of both sexes was found to contain polypeptide with mr, close to mr of purified uebp (approximately 31,000), which reacts with anti-uebp specific antiserum. detection of the immunoreactive material in female liver, which does not contain any hormone-bind ...	1989	2804329
detection of factor x activation in humans.	a sensitive radioimmunoassay (ria) for the fragment that is liberated from factor x when this zymogen is activated by factor vii/viia-tissue factor or factor ixa was developed. antisera were raised in rabbits to a synthetic 15 amino acid peptide containing the cooh-terminal sequence of the activation fragment coupled to bovine serum albumin with glutaraldehyde. the reactivity of the antibody population obtained toward the factor x zymogen was negligible (less than 1/36,000 that of the activation ...	1989	2804345
detection of factor x activation in humans.	a sensitive radioimmunoassay (ria) for the fragment that is liberated from factor x when this zymogen is activated by factor vii/viia-tissue factor or factor ixa was developed. antisera were raised in rabbits to a synthetic 15 amino acid peptide containing the cooh-terminal sequence of the activation fragment coupled to bovine serum albumin with glutaraldehyde. the reactivity of the antibody population obtained toward the factor x zymogen was negligible (less than 1/36,000 that of the activation ...	1989	2804345
properties of cutaneous afferents in diabetic neuropathy.	the technique of percutaneous microneurography was used to study the response properties of 68 cutaneous afferents in 8 diabetic patients and, for comparison, of 104 afferents in 12 normal control subjects. the diabetic patients were neurologically asymptomatic or had a mild sensory neuropathy. three afferent categories were studied in the diabetic subjects (rapidly adapting type ra and slowly adapting sai and saii) in response to mechanical skin stimulation and abnormal responsiveness was encou ...	1989	2804617
formation and removal of dna adducts in target and nontarget tissues of rats administered multiple doses of 2-acetylaminophenanthrene.	2-acetylaminophenanthrene (2-aap) is carcinogenic for the mammary gland, small intestine and ear duct in rats, but is not hepatocarcinogenic. in order to understand the tissue specificity of tumor induction, the formation and removal of dna adducts in target and nontarget tissues have been compared in rats administered 2-aap. male and female sprague--dawley rats were treated i.p. with up to four weekly doses of 5 mg 2-aap per kg body weight. 32p-post-labeling analysis of the dna from all tissues ...	1989	2805226
formation and removal of dna adducts in target and nontarget tissues of rats administered multiple doses of 2-acetylaminophenanthrene.	2-acetylaminophenanthrene (2-aap) is carcinogenic for the mammary gland, small intestine and ear duct in rats, but is not hepatocarcinogenic. in order to understand the tissue specificity of tumor induction, the formation and removal of dna adducts in target and nontarget tissues have been compared in rats administered 2-aap. male and female sprague--dawley rats were treated i.p. with up to four weekly doses of 5 mg 2-aap per kg body weight. 32p-post-labeling analysis of the dna from all tissues ...	1989	2805226
fatty-acid-binding protein from bovine brain. amino acid sequence and some properties.	a fatty-acid-binding protein (fabp) from the cytosol of bovine brain was purified by sephadex g-75 filtration and electrofocusing. the purified fabp behaved as an anionic protein with an apparent molecular mass of 14.7 kda; its complete amino acid sequence was determined and microheterogeneity was observed. sequence comparison with other fabps of known sequence and the observed microheterogeneity demonstrated the presence in brain of several homologous fabps closely related to heart fabp and bov ...	1989	2806261
pancreaticoduodenal graft in the rat: an original microsurgical technique.	the authors describe an original surgical technique for pancreatic transplantation carried out on an experimental rat model which duplicates as closely as possible the anatomic-functional situation of a pancreatic graft in man (one arterovenous vascular peduncle with one functioning kidney only). the technique entails a microanastomosis between the mesenteric artery and the portal vein of the donor organ with the left renal artery and vein of the recipient. this method appears to be technically ...	1989	2806343
[the effect of progesterone, testosterone and estradiol on steroid hormone release by the ovarian follicles of cows in vitro].		1989	2806660
[study of polymorphism of strains of vibrio cholerae of various origins using a method of genomic fingerprinting].	population of atoxigenic eltor vibrios (vct-) isolated from open water has been shown to be heterogeneous by the genomic fingerprinting technique. epidemically dangerous strains (vct+) containing the gene coding for cholera toxin have similar hybridization profile independent of time and place of their isolation. the clonal formation of vct+ strains and the absence of vct----vct+ transition of strains under natural conditions are discussed.	1989	2806903
molecular characterization of trp1, a gene coding for tryptophan synthetase in the basidiomycete coprinus cinereus.	we utilized a cloned gene (trp5) encoding tryptophan synthetase (tsase) from saccharomyces cerevisiae to identify and clone the corresponding gene (trp1) from the basidiomycete coprinus cinereus. the primary nucleotide (nt) sequence of this gene was determined and compared to sequences from other filamentous fungi, as well as to other genes coding for tsase. a transformation assay was used to demonstrate that 321 nt, which do not include caat or tataaa elements and precede the translation initia ...	1989	2806911
preliminary assessment of the safety and immunogenicity of live oral cholera vaccine strain cvd 103-hgr in healthy thai adults.	a single dose (5 x 10(8) organisms) of attenuated a- b+ vibrio cholerae classical inaba recombinant vaccine strain cvd 103-hgr or placebo was administered to 24 healthy young thai adults in a randomized, placebo-controlled, double-blind trial of safety and immunogenicity. none of the volunteers experienced untoward reactions. the vaccine strain was recovered from 2 of 12 vaccines. the vibriocidal antibody response (the best immunological correlate of protection) was good: 11 of 12 vaccinees (92% ...	1989	2807523
development of location-specific hair cell stereocilia in denervated embryonic ears.	the developmental mechanisms that allow physiological coding of acoustic pitch have remained unexplained. cochlear hair cells that have different structures respond to different sound frequencies and synapse with neurons that project to different locations in the brain. how do these hair cells develop appropriate structures, and how are the connections between specific hair cells and the neurons that code for their pitch sensitivities matched? we have investigated one aspect of this by denervati ...	1989	2808748
states of the art of immunoelectron microscopy in japan.	in japan, there are several laboratories which routinely utilize immunoelectron microscopy (iem) for their investigation and participate in the development of methods for iem. as a result, some major advances are in the making. iem methods used in japan may be divided roughly into four categories: 1) antibodies are reacted with tissues before the tissues are embedded in plastics (pre-embedding method); 2) antibodies are reacted with tissues after the tissues are embedded in plastics (post-embedd ...	1989	2809470
regulation of insulin-like growth factor-i and growth hormone receptor gene expression by diabetes and nutritional state in rat tissues.	insulin-like growth factor-i (igf-i) mrna and gh receptor mrna levels were analysed in different tissues from rats made diabetic with streptozotocin, fasted rats and rats fed with a protein-reduced diet. diabetes decreased igf-i mrna levels in liver, heart, diaphragm, kidney and aorta, but not in brain. gh receptor mrna levels were decreased in heart and diaphragm, but not in liver and kidney. fasting decreased igf-i mrna in all tissues studied except brain, and decreased gh receptor mrna in liv ...	1989	2809476
an antitumor principle from suillus granulatus.	an antitumor principle from suillus granulatus has been identified as suillin. in vitro and in vivo activities against p-388 leukemia have been determined.	1989	2809609
thyroid follicular carcinoma with pulmonary metastases in a beaver (castor canadensis).	an 11-yr-old female beaver (castor canadensis) died after a 3 1/2 mo course of intermittent diarrhea, lethargy and anorexia. a postmortem examination revealed both a necrotizing ulcerative colitis and bilaterally enlarged thyroid glands. histologically, the necrotizing colitis was similar to that caused by canine or feline parvovirus. thyroid glands were multilobulated. lobules were composed of irregularly arranged, variably sized follicles, some of which contained colloid. follicles were lined ...	1989	2810561
[clinical laboratory approach for estimating effective administrative dose of cephalothin. reevaluation of mic break points in 4 category system of disc susceptibility test].	the reliability of the cephalothin (cet) disc susceptibility test in estimating approximate values of mics was studied using various clinical isolates totaling 248 strains and using showa discs (8 mm diameter containing 30 micrograms of cet) and difco discs (6 mm diameter containing 30 micrograms of cet). clinical significance of a 4 category system for the interpretation of the cet disc tests, which is widely used in japan, was reevaluated to determine whether this system would be suitable or n ...	1989	2810753
cathepsin b in cells of two rat sarcomas with different rates of spontaneous metastasis.	the cysteine proteinase cathepsin b (ec 3.4.22.1) is believed to take part in biochemical processes underlying tumor metastasis. in the present study, the cellular localization, intracellular levels and extracellular release of cathepsin b activity were examined in vitro in cells of two rat sarcoma variants, lw13k2 and rps, differing in their capacity to metastasize spontaneously to the lung of syngeneic lew/cub rats. the lw13k2 sarcoma metastasizes rarely, whereas the lw13k2-derived rps variant ...	1989	2812149
cathepsin b in cells of two rat sarcomas with different rates of spontaneous metastasis.	the cysteine proteinase cathepsin b (ec 3.4.22.1) is believed to take part in biochemical processes underlying tumor metastasis. in the present study, the cellular localization, intracellular levels and extracellular release of cathepsin b activity were examined in vitro in cells of two rat sarcoma variants, lw13k2 and rps, differing in their capacity to metastasize spontaneously to the lung of syngeneic lew/cub rats. the lw13k2 sarcoma metastasizes rarely, whereas the lw13k2-derived rps variant ...	1989	2812149
role of carboxylesterase in soman, sarin and tabun poisoning in rats.	tocp (triorthocresyl phosphate) inhibits carboxylesterase (carbe) activity in rat plasma and liver and significantly increases soman, sarin and tabun toxicity. application of these agents after tocp caused strong additional inhibition of carbe and cholinesterases (che) in rat red blood cells, plasma, liver, brain, diaphragm and intercostal muscle. after phenobarbital pretreatment, which induced carbe activity in plasma and liver by 80% and that of plasma che by 33%, acute toxicity of soman and t ...	1989	2813290
etiology of acute childhood diarrhoea in calcutta.	of the 152 cases of acute diarrhoea, 124 (81.5%) revealed potential pathogens. altogether 27 (21.2%) out of 127 strains of escherichia coli, klebsiella pneumoniae, enterobacter, proteus and acinetobacter produced enterotoxin. single pathogenic bacteria (40 cases 26.3%), parasite (6; 6%), rota virus (6; 6%), toxigenic bacteria (19; 12.5%) and mixed agents (37; 24.24.3%) were recorded in 108 cases (71.0%). another 14 (9.2%) cases exclusively revealed moderate to heavy growth of suspected enteric p ...	1989	2815324
differential inhibition of downstream gene expression by the cauliflower mosaic virus 35s rna leader.	the effect of the 600 nucleotide-long camv 35s rna 5' leader sequence on the expression of downstream genes was analyzed both in plant protoplasts and in vitro. for transient expression studies in protoplasts derived from host and nonhost plants, the bacterial chloramphenicol acetyl transferase (cat) gene was fused to the initiation codon of orf vii. the leader sequence reduced cat expression two- to four-fold in protoplasts derived from three host species, but 10- to 50-fold in protoplasts deri ...	1989	2815595
differential inhibition of downstream gene expression by the cauliflower mosaic virus 35s rna leader.	the effect of the 600 nucleotide-long camv 35s rna 5' leader sequence on the expression of downstream genes was analyzed both in plant protoplasts and in vitro. for transient expression studies in protoplasts derived from host and nonhost plants, the bacterial chloramphenicol acetyl transferase (cat) gene was fused to the initiation codon of orf vii. the leader sequence reduced cat expression two- to four-fold in protoplasts derived from three host species, but 10- to 50-fold in protoplasts deri ...	1989	2815595
[establishment and primary application of non-oi vibrio cholerae serotyping system (vbo)].	a serotyping system for vibrio cholerae non-oi was reported in this paper. with this typing system, 549 strains isolated from guangdong province, fujian province and henan province were typed. the 55 serotypes were found in these strains. among this, the dominant types were vbo 2, 7 and 9. the distribution of serotypes related with the different region. in our experiment, 19 serotypes were new types, different from sakazaki or smith strains. 83.79% tested strains can be typed with this vbo serot ...	1989	2815719
the dermatopathologist and human immunodeficiency virus infection.		1989	2817921
[visualization of forensically important foreign bodies by direct roentgen magnification].	the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ...	1989	2818477
[visualization of forensically important foreign bodies by direct roentgen magnification].	the detection of foreign bodies of microscopic dimensions with radiographic techniques is limited by the size and density of the particle and the radiographic resolution. these restrictions can be of decisive value in case work. the high grade direct radiographic magnification technique was successfully used in preceding experiments. due to its 1 mu focal spot, this tube allows direct magnifications up to 250 times. in present studies, the advantages as well as the limitations of this new techni ...	1989	2818477
production and partial characterization of elastase of vibrio cholerae inaba.	conditions are described for the production of extracellular elastase by vibrio cholerae inaba 2/57. the yield of the enzyme was maximum in shake cultures grown in alkaline peptone water at 37 degrees c and was stable in culture supernatants. the enzyme, partially purified by ammonium sulphate precipitation and sephadex g-100 gel filtration showed a molecular weight of 30,000 and an activity between ph 5.0-8.0 with an optimum of 8.0 in tris-maleate buffer. the elastinolytic activity was maximal ...	1989	2818795
comparison of the structural characteristics of cell-cam 105 from hepatocytes with those of an altered form expressed by rat transplantable hepatocellular carcinomas.	the structural features of the adult rat hepatocyte (arh) forms of cell-cam 105, a mr 105,000 cell adhesion molecule, were compared using a variety of immunochemical and biochemical techniques with altered forms of more basic pi present on two transplantable hepatocellular carcinomas (thc 1682c and thc as-30d). immunoprecipitation analysis with polyclonal (anti-gp 105-2) and monoclonal (mab) antibodies specific for cell-cam 105 (mab 362.50) demonstrated that arh and thc cell-cam 105 were indisti ...	1989	2819719
abortive replication of choleraphage phi 149 in vibrio cholerae biotype el tor.	choleraphage phi 149 adsorbed irreversibly to vibrio cholerae biotype el tor cells, and 50% of the injected phage dna bound to the cell membrane. although no infectious centers were produced at any time during infection, the host macromolecular syntheses were shut off and the host dna underwent chloramphenicol-inhibitable degradation. synthesis of monomeric phage dna continued similar to that observed in the permissive host. however, the concatemeric dna intermediates produced were unstable and ...	1989	2908925
immunogenicity of two formulations of oral cholera vaccine comprised of killed whole vibrios and the b subunit of cholera toxin.	two formulations of oral cholera vaccine were evaluated for safety and immunogenicity in adult volunteers in thailand, an area with sporadic cholera outbreaks. one formulation consisted of 2 x 10(11) killed vibrios and 5 mg of cholera toxin b subunit, as was previously evaluated in north american volunteers, and the other consisted of 1 x 10(11) killed vibrios and 1 mg of b subunit, as was recently evaluated in a field trial in bangladesh. three doses of each formulation were given with citrate- ...	1989	2909484
immunogenicity of a candidate live oral typhoid/cholera hybrid vaccine in humans.		1989	2909633
relationship between extracellular matrix interactions and degree of differentiation in human colon carcinoma cell lines.	human colon carcinoma cell lines that vary in their degree of differentiation were examined for their ability to interact with extracellular matrix components. for this purpose, established cell lines were classified on the basis of several criteria that relate to degree of differentiation. these criteria include histology of the original tumor, histology of xenografts, in vitro morphology, and carcinoembryonic antigen expression. on this basis, the cell lines used were either moderately well or ...	1989	2910488
effect of ether or ketamine anesthesia on rat uterine estrogen and progesterone receptors.	rat uterine estrogen receptors (er) and progesterone receptors (pr) have been used as controls in er and pr assays of breast tumors. stunning or decapitation of experimental animals without prior anesthesia is no longer acceptable as a method of killing. thus, we compared the effects of two anesthetics on the concentration of rat uterine er and pr. rats were killed by one of three methods: (a) stunning, (b) ether anesthesia followed by decapitation, or (c) ketamine anesthesia followed by decapit ...	1989	2910556
polyclonal anti-idiotypes influence macrophage chemotaxis in coxsackievirus-induced murine myocarditis.	investigation into the mechanism(s) whereby monocytes-macrophages are mobilized to the myocardium of coxsackievirus (cvb3)-infected mice was approached by assessing the role of anti-idiotypic antibodies against specific antiviral igg molecules. balb/c mice preinoculated with polyclonal anti-idiotypic, syngeneic antibodies resulted in significantly decreased myocarditis after virus challenge. on the other hand, oil-induced peritoneal exudate cells obtained from virus-exposed animals demonstrated ...	1989	2911021
dexamethasone for bacterial meningitis in children.		1989	2911250
an experimental study of susceptibility to infection after hemorrhagic shock.	hemorrhagic shock has been associated with an increased risk of infection after injury. the immediate and long term effects of hemorrhagic shock without tissue injury on the susceptibility of an animal to infection and the efficacy of antibiotic prophylaxis to prevent infection in this setting were examined. sprague-dawley rats were subjected to hemorrhagic shock (ld15) by bleeding to a mean arterial pressure of 45 millimeters of mercury for 45 minutes and were resuscitated with shed blood and n ...	1989	2911791
human immune response to vibrio cholerae o1 whole cells and isolated outer membrane antigens.	the serum immunoglobulin g (igg) and mucosal secretory iga (siga) response of human volunteers challenged with vibrio cholerae o1 was analyzed for reactivity to v. cholerae o1 antigens by the immunoblot technique. components of both in vitro- and in vivo (rabbit ligated ileal loop)-grown v. cholerae o1 were separated by sodium dodecyl sulfate-urea-polyacrylamide gel electrophoresis. postchallenge serum igg reacted uniquely with 15 antigens and with greater intensity than did prechallenge serum w ...	1989	2912896
rapid identification of vibrio cholerae serotype o1 from primary isolation plates by a coagglutination test.	a coagglutination test was developed for identifying suspected colonies of vibrio cholerae serotype o1 directly from primary isolation plates. visible agglutination occurs when v. cholerae o1 antibody attached to cell-wall protein a of staphylococcus aureus reacts with its homologous antigen. from 314 faecal samples from clinically suspected cases of cholera, 210 colonies from thiosulphate citrate bile salts sucrose (tcbs) agar and 222 colonies from taurocholate tellurite gelatin (ttg) agar were ...	1989	2913315
an adaptive response of vibrio cholerae strain ogawa 154 to furazolidone.	since furazolidone is an antimicrobial drug, any possibility of its evoking an adaptive response appears to be very important. this response was studied in vibrio cholerae cells as a model system. in order to determine this response, a dose-response relation of these cells to furazolidone and the kinetics of inactivation of the drug were studied. the study of the adaptive response of these cells to furazolidone reveals that cells treated with a low concentration of furazolidone for a particular ...	1989	2913490
the astroglial response to autonomic tissue grafts.	autonomic (superior cervical) ganglia were grafted either into the iv ventricle where minimal trauma occurred or directly into the cerebral cortex which was necessarily traumatic. previous studies have shown that host astroglia may migrate into autonomic tissue grafts. the purpose of the present study was to compare and contrast the astroglial response in allo- and autografts. by monitoring the host response in the two model sites using glial fibrillary acidic protein (gfap) immunostaining in 1 ...	1989	2914205
improved sexual function in hemodialysis patients on recombinant erythropoietin: a possible role for prolactin.	as it was reported that correction of anemia in long-term hemodialysis patients by recombinant human erythropoietin (r-huepo) is associated with improved sexual function, we conducted the present study to further delineate the mechanism(s) by which this is brought about. serum prolactin, testosterone, and parathyroid hormone (pth) levels were followed during 4 months of r-huepo therapy. within 4 months of treatment with r-huepo, hematocrit values rose from 23.7 +/- 1.2 to 35.7 +/- 0.2% and hemog ...	1989	2914405
one of two subunits of masking protein in latent tgf-beta is a part of pro-tgf-beta.	a high molecular mass latent form of transforming growth factor type-beta (tgf-beta) was purified to homogeneity from rat platelets by a seven-step procedure involving group-specific affinity chromatographies on red-toyopearl and zinc chelating-sepharose. the purified latent tgf-beta was a complex of tgf-beta (25 kda) and the binding protein previously named masking protein (approximately 400 kda) [(1986) biochem. biophys. res. commun. 141, 176-184]. analysis of the peptide structure by gel elec ...	1989	2914605
the biochemical and pathological changes produced by the intratracheal instillation of certain components of zinc-hexachloroethane smoke.	zinc chloride which is formed by igniting a mixture of zinc oxide and hexachloroethane in the production of white smokes has been shown to produce oedema when given to rats as a single instillation. the oedematous reaction, as assessed by histopathology and measurements of alveolar surface protein in lavage fluid, is variable, dose-dependent, and maximal at 3 days but at sub-lethal doses it regresses after 7 days. the parent compound, zinc oxide, does not produce these effects. in some animals t ...	1989	2916244
saturable binding sites mediate the entry of african swine fever virus into vero cells.	binding experiments of 3h-labeled african swine fever virus to susceptible vero cells have shown the presence of saturable binding sites for african swine fever virus on the plasma membrane. the scatchard analysis of the binding data at equilibrium indicates the existence of about 10(4) cellular receptor sites per cell with a dissociation constant (kd) of 70 pm. virus entry into vero cells is mediated by a saturable component, since tritiated african swine fever virus saturable binding and uptak ...	1989	2916331
thyroid function in hyperemesis gravidarum.	plasma total t4 (tt4), t3 (tt3), free t4 (ft4), free t3 (ft3), thyroxine binding globulin, hcg, and erythrocyte zinc content were measured in 43 women with uncomplicated pregnancy and in 71 patients admitted with hyperemesis gravidarum. plasma concentration of thyroid hormones in hyperemesis subjects showed wide variability and 32% of subjects had high tt4 (higher than mean +2 sd of normal pregnant subjects), 33% had high ft4, 20% had high tt3, and 20% had high ft3. red cell zinc content, a tiss ...	1989	2916376
binding of cholera toxin to pig intestinal mucosa glycosphingolipids: relationship with the abo blood group system.	a search for compounds from intestinal mucosa of pigs carrying and not carrying blood group a-active substances (a+ and a- pigs, respectively) capable of binding cholera toxin (ct) was performed. glycolipid extracts from a pool of pig intestinal mucosa resolved in thin-layer chromatography (tlc) revealed the presence of six to eight compounds capable of binding 125i-ct, two of them running as the ganglioside standards gm1 and gd1b. when intestinal mucosa glycolipids from single pigs were assayed ...	1989	2917796
intestinal maturation: characterization of mitochondrial calcium transport in the rat.	the mitochondria play a major role in the regulation of intracellular calcium. despite the fact that the enterocytes receive the majority of absorbed calcium, the role of the intestinal mitochondria in calcium transport during maturation is not known. therefore, the current studies were designed to characterize calcium pump activity of jejunal mitochondria of rats during maturation (suckling, weanling, and adolescent rats). the functional integrity of the intestinal mitochondria of suckling and ...	1989	2919110
hepatic structural correlates of liver fibrosis: a morphometric analysis.	liver fibrosis is an important feature of many liver diseases, and the assessment of fibrosis is essential for diagnosis and prognosis. liver needle biopsy, however, tends to sample preferentially the soft parenchyma, which poorly reflects the true extent of fibrosis. therefore, we have searched for capsule and parenchymal features that are correlated with the fibrosis. to test for these correlations, we used a morphometric analysis of a rat model of liver fibrosis. we demonstrated that, of the ...	1989	2920817
bovine lactogenic immunity against cholera toxin-related enterotoxins and vibrio cholerae outer membranes.	the newly parturient cow secretes large quantities of immunoglobulin g1, a relatively protease- and heat-resistant immunoglobulin, in its colostrum and milk. this study establishes the feasibility of producing protective colostral immunoglobulins by immunizing pregnant cows with cholera toxin (ct), a ct-related enterotoxin from escherichia coli, and vibrio cholerae outer membranes (oms). the oms were prepared from bacteria grown under iron-replete or iron-deficient (to simulate the in vivo envir ...	1989	2925248
nontoxigenic vibrio cholerae wound infection after exposure to contaminated lake water.		1989	2926177
the influence of alpha-tocopherol and pyritinol on oxidative dna damage and lipid peroxidation in human lymphocytes.	unscheduled dna synthesis (uds) and lipid peroxidation (lpo) were measured in human peripheral lymphocytes from healthy volunteers. these processes were induced by the catalytic system fe2+-sodium ascorbate. the degree of induced lpo was measured spectrophotometrically by the thiobarbituric acid assay. uds was detected by scintillometric measurement of the incorporation of 3h-thymidine into dna. the protective action by fat-soluble vitamin e (d,l-alpha-tocopherol) and the artificial antioxidant ...	1989	2927430
electron microscopic localization of cholinergic terminals in the rat substantia nigra: an immunocytochemical study.	the presence of cholinergic terminals in the substantia nigra (sn) of the rat was investigated under the electron microscope using a monoclonal antibody against choline acetyltransferase (chat), the acetylcholine (ach)-synthesizing enzyme, following the unlabelled antibody peroxidase-antiperoxidase (pap) procedure. chat-immunoreactive terminals were found making synaptic contacts with unlabelled dendrites in the sn pars compacta (snc). synaptic contacts established between cholinergic boutons an ...	1989	2927715
central vasomotor control of the rabbit portal vein.	contractile responses of the portal vein of the anesthetized rabbit were measured quantitatively by plethysmography during transmural electrical field stimulation (tes, 0.8 ms and 15 v) and during the cerebral ischemic pressor response at various volemic states. to evoke the cerebral ischemic response, the route of blood supply to the brain was surgically restricted to the right internal carotid artery, the artery was then compressed in a stepwise fashion by a micrometer device. the maximum cont ...	1989	2928086
three potassium currents in mouse motor nerve terminals.	a study of the k conductance of the presynaptic membrane has been performed in the triangularis sterni muscle of the mouse. external currents generated in the presynaptic terminals upon invasion by action potentials have been recorded using microelectrodes inserted into the perineurium of preterminal nerve bundles. the voltage-dependent k current could be pharmacologically dissected into fast (ikf) and slow (iks) components. while both are sensitive to 3,4-diaminopyridine (3,4-dap), only ikf is ...	1989	2928092
the nucleotide sequence and reading frames of a mutant hepatitis b virus subtype adr.		1989	2928116
britain launches genome program.		1989	2928800
antigenic analysis of vibrio cholerate o1 by crossed immunoelectrophoresis.	antigens from vibrio cholerae o1 were analyzed by crossed immunoelectrophoresis (cie) using sera from immunized rabbits. thirty different anode-migrating antigens were detected in sonicated antigen preparations of v. cholerae. these antigens were numbered in order to establish a reference precipitation pattern. antigen no. 30 was identified as the lipopolysaccharide (lps) antigen, because it reacted with (i) periodic acid-schiff (pas) reagent and (ii) the affinity-purified anti-lps antibodies. t ...	1989	2929189
prevalence of borrelia burgdorferi in ixodes ricinus ticks in berlin (west).	in 1986, 1711 nymphal and adult ixodes ricinus orginating from berlin (west) forests were examined individually or in pools of up to 10 ticks for the presence of borrelia burgdorferi, the etiologic agent of lyme borreliosis. detection of borreliae was carried out by means of a culture method using modified barbour-stoenner-kelly-medium (bsk ii). tick populations from 14 out of 15 locations contained positive specimens. the calculated minimal infection rate of pooled ticks was 2.5% in nymphs (n = ...	1989	2929194
nontraditional pedagogy for implementing human genetics education.		1989	2929601
twinning in human populations and in cattle exposed to air pollution from incinerators.		1989	2930734
rapid enumeration of viable bacteria by image analysis.	a direct viable counting method for enumerating viable bacteria was modified and made compatible with image analysis. a comparison was made between viable cell counts determined by the spread plate method and direct viable counts obtained using epifluorescence microscopy either manually or by automatic image analysis. cultures of escherichia coli, salmonella typhimurium, vibrio cholerae, yersinia enterocolitica and pseudomonas aeruginosa were incubated at 35 degrees c in a dilute nutrient medi ...	1989	11536935
president's message.		1989	267150
[oral changes due to hydantoin].		1989	267142
metiamide: more than an h2-receptor antagonist.	the effect of metiamide on acid secretion and calcium uptake by the frog gastric mucosa was tested alone and in combination with histamine, pentagastrin, aminophylline, and dibutyrl cyclic amp (db camp). metiamide inhibited all stimulants including db camp which presumably acts intracellularly beyond the h2 receptor. metiamide, therefore, actively inhibits at a point within the cell beyond the adenylated cyclase membrane receptor complexes. metiamide also increases calcium influx into the pariet ...	1989	203185
a chloroplast photosystem 2 reaction resistant to salicylaldoxime.		1989	203131
[stimulation of the periaqueductal gray matter for pain : electrical activity and evoked potentials (author's transl)].	there is some evidence that stimulation of the periaqueductal gray matter produced analgesia is accomplished by the activation of neuronal systems where endorphins are thought to be transmittors. however, the neurophysiological mechanism of this stimulation is not elucided. in this preliminary paper, the spontaneous and the evoked electrical activity has been studied in 3 patients operated on with implantation of an electrode in the periaqueductal gray matter for chronic pain. particularly inter ...	1989	313529
[an attempt to ease the urination difficulty following total hysterectomy].		1989	172681
last opportunity.		1989	267697
postgraduation plans reported by the university of louisville's 1976 graduates.		1989	267696
interferon alpha-induced changes in fc gamma r-specific mrna expression and isotype-specific, fc gamma r-mediated phagocytosis in c3h/ouj (lpsn) and c3h/hej (lpsd) macrophages.	peritoneal exudate macrophages from c3h/ouj (lpsn) and c3h/hej (lpsd) mice were treated with interferon alpha (ifn-alpha), and isotype-specific phagocytosis and gene expression were measured. ifn-alpha selectively augments phagocytosis of igg2a, with a highly significant induction of fc gamma ri mrna expression, while parallel fc gamma riii alpha measurements show a relatively small increase.	1989	1541912
the influence of rearing density on the subsequent response to ddt dosing for tadpoles of the frog rana temporaria.		1989	313824
[action of calcitonin on osteoclasts in paget's bone disease during long term treatment (author's transl)].	comparison of biopsies from patients with paget's bone disease before and during long-term treatment with calcitonin reveals that the ration of bone-adherent osteoclasts to free osteoclasts is not modified by the hormone. nor does treatment alter the average number of nuclei in osteoclasts. under electron microscopy, all the cytological anomalies of osteoclasts in paget's bone disease and, in particular, the characteristic nuclear inclusions, persist through treatment. thus, although such osteoc ...	1989	202910
drugs in current use: gout.		1989	313207
peritoneal dialysis clearance of cimetidine.	peritoneal dialysis clearance of cimetidine was determined in two patients undergoing peritoneal dialysis treatment. the peritoneal dialysis clearance of cimetidine was approximately 10 ml./min. under optimal dialyzing conditions. the average cimetidine to creatinine extraction ratio was 0.45, which represents significant peritoneal dialyzability. the amount of cimetidine removed, however, constituted only a small fraction of the total administered dose. therefore, only minor dose adjustments ma ...	1989	313152