Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| rapid gm1-enzyme-linked immunosorbent assay with visual reading for identification of escherichia coli heat-labile enterotoxin. | a modified, quicker and simpler gm1-enzyme-linked immunosorbent assay procedure for detection of escherichia coli heat-labile enterotoxin (lt) has been developed with the intent that it should be useful in less well-equipped laboratories. the method, which makes use of stable reagents including commercially available horseradish peroxidase immunoglobulin conjugate, can detect lt in overnight cultures within 1 working day (8 h), and the tests can be read with the naked eye. this gm1-horseradish p ... | 1983 | 6343419 |
| detection of igm antibodies to measles virus by enzyme-immunoassay. | a method of solid-phase enzyme-immunoassay (eia) with horseradish-peroxidase-conjugated anti-mu-globulin was used to determine igm antibodies to measles virus in human sera. antigens prepared from measles-infected and noninfected vero cells passively adsorbed to polystyrene cuvettes were both important for the tests, because sera from many patients convalescing from viral infections and sera from rheumatoid arthritis patients were able to bind to control antigen. sera from measles patients, pati ... | 1983 | 6343817 |
| labelling of an intermediate saccule of the golgi apparatus and of parts of the endoplasmic reticulum by a lectin (soybean agglutinin) in the chick ciliary ganglion. | a lectin (soybean agglutinin or sba) conjugated with horseradish peroxidase was used to identify the intracellular membranes containing galactose or alpha-n-acetylgalactosamine in young chick ciliary ganglions. the label is found on one or, less frequently, on two saccules of the golgi apparatus in the ciliary and choroid neurones. the labelled intermediate saccules are always located either near the cisface or the middle region of the golgi apparatus. this observation confirms that distinct com ... | 1983 | 6343928 |
| maintenance cultures of kupffer cells isolated from rats of various ages: ultrastructure, enzyme cytochemistry, and endocytosis. | sinusoidal liver cells were isolated from the livers of 3-, 12-, 30-, and 36-month-old female bn/birij rats by enzymatic digestion. the kupffer cells in the sinusoidal cel suspensions were purified by centrifugal elutriation and kept in maintenance culture for periods of up to about 3 weeks. the viability and yield of kupffer cells per gram of body weight did not change with the age of the donor rat. the ultrastructural, cytochemical, and functional characteristics of kupffer cells as observed i ... | 1983 | 6345331 |
| detection of soluble antigens of toxoplasma gondii by a four-layer modification of an enzyme immunoassay. | a sensitive four-layer modification of an enzyme immunoassay for the detection of soluble antigens of toxoplasma gondii is described. microtiter plates were sensitized with rabbit anti-toxoplasma immunoglobulins (6 micrograms/ml) used as the primary antibodies; guinea pig anti-toxoplasma immunoglobulins (6 micrograms/ml) were used as the secondary trapping antibodies. horseradish peroxidase-conjugated anti-guinea pig immunoglobulins were used as the indicator antibodies. the specificity of the a ... | 1983 | 6345574 |
| morphology of cerebral endothelium and astrocytes as determinants of the neuronal microenvironment. | the morphological features of the blood-brain barrier to macro-molecules under normal and perturbed conditions are reviewed in the context of some recent investigations. the electric charge on molecules of horseradish peroxidase (hrp) affect its pinocytosis and intracellular fate which pertains to problems of distinguishing endocytosis from vesicular transport across endothelium. when the barrier is opened, the number of pits, vesicles and tubules increases. such cerebral endothelium resembles n ... | 1983 | 6346778 |
| immunocytochemical demonstration of gabaergic synapses on identified rubrospinal neurons. | gabaergic synapses on rubrospinal neurons were demonstrated with immunocytochemistry combined with intracellular injection of horseradish peroxidase. sections containing red nucleus neurons were processed for glutamic acid decarboxylase (gad) immunohistochemistry. gad-immunoreactive synaptic endings formed synaptic contacts with somata and dendrites of red nucleus neurons and identified rubrospinal neurons. our observation provides further evidence that gaba acts as an inhibitory transmitter med ... | 1983 | 6347337 |
| immunocytochemical localization of acrosin on both acrosomal membranes and in the acrosomal matrix of porcine spermatozoa. | immunocytochemical techniques were employed to determine, at the ultrastructural level, the location of acrosin in porcine spermatozoa. antisera to highly purified porcine acrosin was produced in rabbits. the (fab')2 fragments of the immunoglobulins were purified and conjugated with horseradish peroxidase (hrp). washed, formaldehyde-fixed spermatozoa were reacted with the labeled antiacrosin immunoglobulins, utilizing a direct staining technique. electron microscopy revealed that the peroxidase ... | 1983 | 6348010 |
| endotoxin contamination of enzyme conjugates used in enzyme-linked immunosorbent assays. | the specificity of the enzyme-linked immunosorbent assay(s) is thought to depend on the specificity of the antibody used in the assay system. therefore, the association of broadly reactive antigens like endotoxin with enzyme conjugates or other enzyme-linked immunosorbent assay reagents has the potential of altering the specificity of reactions in the enzyme-linked immunosorbent assay. using the limulus amoebocyte lysate assay, we demonstrated that commercially prepared conjugates of goat anti-h ... | 1983 | 6348074 |
| enzyme-linked immunosorbent assay that uses labeled antigen for detection of immunoglobulin m and a antibodies in toxoplasmosis: comparison with indirect immunofluorescence and double-sandwich enzyme-linked immunosorbent assay. | a direct enzyme-linked immunosorbent assay (elisa) is described that uses horseradish peroxidase-labeled antigen for detection of immunoglobulin m (igm) and iga antibodies to toxoplasma. in this assay, polystyrene microtiter plates were sensitized with anti-human igm or iga antibody to separate igm or iga from other classes of antibody. the presence of igm or iga antibodies to toxoplasma (tox-igm, tox-iga) was then detected by sequential addition of soluble horseradish peroxidase-labeled toxopla ... | 1983 | 6348083 |
| specificity of muscle reinnervation after epineurial and individual fascicular suture of the rat sciatic nerve. | we experimentally analyzed the specificity of muscle reinnervation after suture and regeneration of rat sciatic nerve. we used a horseradish peroxidase (hrp) technique of axon tracing to compare the number and location of motoneurons that innervate muscle via the peroneal nerve after epineurial and individual fascicular suture of the parent sciatic nerve. these motoneurons are significantly reduced in number from control levels and are often in spinal cord locations that indicate previous innerv ... | 1983 | 6348148 |
| immunocytochemical localization of the functional fraction of lipoprotein lipase in the perfused heart. | the functional (heparin-releasable) fraction of myocardial lipoprotein lipase (lpl) has been located at the lumen surface of capillary endothelium by means of an indirect immunocytochemical perfusion method for electron microscopy. the primary step immunoreactant was an igg fraction of goat antiserum directed against lpl from rat heart. the second step antibody, conjugated with horseradish peroxidase, was rabbit igg directed against goat igg. peroxidase reaction product, when present, appeared a ... | 1983 | 6348194 |
| improved visualization of neurons labeled with horseradish peroxidase: silver-intensification of the pyrocatechol/p-phenylenediamine reaction product. | a silver intensification procedure suitable for use with pyrocatechol/p-phenylenediamine (pc-ppd) product of the horseradish peroxidase (hrp) reaction is described. qualitative and quantitative results from retrogradely labeled neurons in the cat cortex after thalamic injection of hrp demonstrate an increase of the intensity of labeling and in the number of darkly labeled cells after the intensification procedure. in both the non-intensified and the intensified pc-ppd reacted tissue the sensitiv ... | 1983 | 6348427 |
| compatibility of horseradish peroxidase tracing with the histochemical demonstration of oxidoreductases. | we have shown the possibilities of combining methods for the histochemical demonstration of oxidative enzymes and horseradish peroxidase such that both activities can be seen in the same cell. this opens a way of studying connectivities of neurons characterized by their complement of certain oxidoreductases. | 1983 | 6348428 |
| localization of horseradish peroxidase (hrp)-anti-hrp complexes in cryostat sections: influence of endotoxin on trapping of immune complexes in the spleen of the rat. | cryostat sections of the rat spleen were incubated with a medium containing horseradish peroxidase (hrp)-anti-hrp complexes. after this incubation hrp-anti-hrp complexes were demonstrated in the follicle centres in the splenic white pulp. the localization pattern of the hrp-anti-hrp complexes after incubation ("in-vitro trapping") was compared with the localization pattern of 125i bsa-anti-bsa complexes after intravenous injection ("in-vivo trapping"). furthermore, the influence of endotoxin on ... | 1983 | 6349815 |
| a comparison of eight different chromogen protocols for the demonstration of immunoreactive neurofilaments or glial filaments in rat cerebellum using the peroxidase-antiperoxidase method and monoclonal antibodies. | eight different previously described chromogen protocols were evaluated with respect to their sensitivity for the visualization of horseradish peroxidase (hrp) in a peroxidase-antiperoxidase (pap) complex used with the unlabeled antibody method for immunohistochemistry. the protocols were evaluated in a test system that involved the demonstration of immunoreactive neurofilaments (nf) or glial filaments (gf) in paraffin-embedded sections of rat cerebellum using anti-nf or anti-gf monoclonal antib ... | 1983 | 6350434 |
| technique for the simultaneous ultrastructural demonstration of anterogradely transported horseradish peroxidase and an immunocytochemically identified neuropeptide. | in order to extend the information obtainable from ultrastructural studies of synaptic connectivity using either horseradish peroxidase tracing or immunocytochemistry alone, we have developed a method of combining these two procedures. thus it has been possible to examine the characteristics of axon terminals of known origin forming synaptic contacts with cells of identified neuropeptide content. | 1983 | 6350437 |
| cortical relay neurons and interneurons in the n. ventralis posterolateralis of cats: a horseradish peroxidase, electron-microscopic, golgi and immunocytochemical study. | after injections of horseradish peroxidase involving the whole primary (si) and secondary somatosensory (sii) areas of adult cats, 16-21% out of 2220 counted neurons in the nucleus ventralis posterolateralis were unlabelled. the mean areas of perikarya of these neurons varied between 111.8 +/- 32.3 microns2 and 180.8 +/- 48.6 microns2. the size of perikarya of retrogradely-labelled neuron ranged from 256.9 +/- 100.4 microns2 to 409 +/- 163 microns2. retrogradely-labelled and unlabelled neurons w ... | 1983 | 6353272 |
| localization of enkephalin-like immunoreactivity in acetylcholinesterase-positive cells in the guinea-pig lateral superior olivary complex that project to the cochlea. | olivocochlear fibers have been demonstrated to have acetylcholinesterase-positive staining both in brainstem and cochlea. olivocochlear fibres in the cochlea have also been determined to contain enkephalin-like immunoreactivity. in this study, we first determined the source of olivocochlear fibers in the guinea-pig using horseradish peroxidase and wheat germ agglutinin in retrograde transport studies. these cells were then examined for enkephalin-like immunoreactivity followed by acetylcholinest ... | 1983 | 6353274 |
| a simplified elisa method for syphilis. | a new serological method for syphilis using a modified elisa reaction is given. the reaction is performed on polystyrene carriers which have absorbed an ultrasonicated preparation of t. pallidum. the reaction between the antigen and antibody is detected by means of the addition of antihuman igg swine globulin conjugated with horseradish peroxidase. to make the reaction visible, addition of 5-methyl-9-aminocarbazol in dimethylformamide is necessary. in a positive case of syphilis the carrier beco ... | 1983 | 6354768 |
| immunoassay for detecting chlamydia trachomatis major outer membrane protein. | the development of a solid-phase immunoassay for the detection of the 39,500-dalton major outer membrane protein of the chlamydia trachomatis lymphogranuloma venereum serotype l2 is described. the test uses immunoadsorbent-purified rabbit anti-l2 major outer membrane protein immunoglobulin g (igg) passively adsorbed to microtiter plates as a capture antibody. this same igg antibody was either conjugated to horseradish peroxidase or radioiodinated with 125i and used as a probe to detect major out ... | 1983 | 6355147 |
| detection of neuronal tissue from brain grafts with anti-thy-1.1 antibody. | grafting of neural tissue has been used to investigate the ability of central nervous system (cns) axons to elongate into peripheral nerve grafts; to investigate the growth characteristics of material transplanted within the cns; and to determine if grafts producing amines or peptides can replace a natural or experimentally induced depletion of these biogenic molecules. in such experiments donor tissue has been distinguished from host by injection into graft cells of horseradish peroxidase or ra ... | 1983 | 6355857 |
| electron microscopic comparison of mouse thymocytes and yolk sac cells using a peanut agglutinin-horseradish peroxidase conjugate. | 1983 | 6357884 | |
| use of avidin-biotinylated horseradish peroxidase complex for visualization of spirochetes. | the use of avidin-biotinylated peroxidase as a simple technique for light microscopic visualization of spirochetes is described. the three major genera of spirochetes--treponema, borrelia, and leptospira--were stained with the avidin complex. | 1983 | 6358252 |
| sensitized (t6 x rfm)f1 donor b cells are engrafted and synthesize antibodies without further antigenic stimulation in rfm recipients with allogenic host-vs-graft disease. | host-vs-graft (hvg) syndrome is the fatal disease of altered immunity that may be induced in susceptible strains of inbred mice by the perinatal inoculation of semiallogenic spleen cells. the allogenic hvg reaction causes severe depletion of both the donor f1 and host t lymphocytes, but the b cell system is hyperplastic and serum immunoglobulins (ig) are markedly elevated. death is caused by glomerulonephropathy and coagulopathy, both thought to be due to immune complexes. previous work indicate ... | 1983 | 6358351 |
| detergent-soaked hrp-chips: a new method for precise and effective delivery of small quantities of the tracer to nervous tissue. | we dissolved horseradish peroxidase (hrp) in a 5% aqueous detergent solution (nonidet p-40) and dried it to a solid mass which was then fragmented into small chips of convenient size. these were applied to the cut or crushed oculomotor nerves of xenopus laevis tadpoles. this method combines the advantages of solid hrp (high concentration) and the presence of a detergent (enhanced uptake). moreover, as compared to chips of hrp alone, addition of the detergent facilitated the manipulation of the c ... | 1983 | 6358709 |
| interaction of lysosomes with endocytic vacuoles in macrophages simultaneously infected with trypanosoma cruzi and toxoplasma gondii. | macrophages with lysosomes previously labelled with acridine orange or horseradish peroxidase were simultaneously incubated in the presence of epimastigotes of trypanosoma cruzi and tachyzoites of toxoplasma gondii. light microscopic observations indicate that although a large number of macrophages were infected by the parasites very few contained both parasites. fusion of the macrophage's lysosomes with vacuoles containing t. cruzi were seen both by fluorescence (acridine orange) and electron ( ... | 1983 | 6361275 |
| localization of s-antigen by enzyme-labelled antibody method and electron microscopy. | localization of s-antigen was studied by a direct enzyme-labeled immunohistochemical method with light and electron microscopes. igg was prepared from rabbits which were sensitized by swine retinal s-antigen and the igg was conjugated with horseradish peroxidase (hrp). this hrp-labeled igg was directly applied to normal rabbit retina and the retina was processed to make slides for light and electron microscopic examinations. in light microscopic findings, a clear-cut positive response was noted ... | 1983 | 6361345 |
| a rapid enzyme-immunoassay for the detection of ciguatoxin in contaminated fish tissues. | an enzyme-immunoassay procedure for the detection of ciguatoxin has been developed using the sheep anti-ciguatoxin serum described earlier in the radioimmunoassay method for ciguatoxin. in the enzyme-immunoassay procedure, the sheep anti-ciguatoxin was coupled to horseradish peroxidase. analyses of fish tissues showed that the enzyme-immunoassay procedure distinguished between clinically documented toxic and nontoxic tissues (p less than 0.001). comparisons of the enzyme-immunoassay method with ... | 1983 | 6362075 |
| development of a direct enzyme immunoassay of milk progesterone and its application to pregnancy diagnosis in cows. | the purposes of this study were to develop an enzyme immunoassay (eia) for determination of progesterone in unextracted whole milk and to apply it to pregnancy diagnosis. paper fibers covalently coupled to antibody specifically and competitively bound 3h-progesterone and 11 alpha-hydroxyprogesterone hemisuccinate-horseradish peroxidase and were stable for 9 mo at -20 degrees c. the sensitivity, recovery, precision, and cross reactivity of the eia and a radioimmunoassay (ria) of milk progesterone ... | 1983 | 6362087 |
| use of inorganic salts to minimize serum interference in a sandwich enzyme immunoassay for human growth hormone using fab'-horseradish peroxidase conjugate. | in a sandwich enzyme immunoassay (eia) for human growth hormone (hgh) with anti-hgh fab'-peroxidase conjugate, the effects of inorganic salts on serum interference were examined, and serum interference was eliminated by incubation of serum samples with anti-hgh igg-coated polystyrene balls in the presence of 0.4 mol/l nacl, avoiding the need for hgh-free serum. the sensitivity for hgh was 60 fg/tube or 3 ng/l of serum. no cross-reaction was observed with prolactin, chorionic gonadotropin or lute ... | 1983 | 6362918 |
| direct pathway from cardiovascular neurons in the ventrolateral medulla to the region of the intermediolateral nucleus of the upper thoracic cord: an anatomical and electrophysiological investigation in the cat. | horseradish peroxidase (hrp) and single unit recording experiments were done in cats to identify neurons in the ventrolateral medulla (vlm) projecting directly to the intermediolateral nucleus (iml) of the thoracic cord and relaying cardiovascular afferent information from the buffer nerves and hypothalamus. in the first series, hrp was allowed to diffuse from a micropipette into the region of the iml at the level of t2. after a survival period of 30-138 h, transverse and horizontal sections of ... | 1983 | 6363504 |
| hepatic-vagal and gustatory afferent interactions in the brainstem of the rat. | in a series of combined electrophysiological and peroxidase histochemical studies, we have established that first-order hepatic and gustatory afferents project to separate regions of the solitary nucleus (nst) and do not converge upon neurons within this structure. additionally, we have found that hepatic vagal afferent fibers travel in the left cervical vagal trunk and project to the postero-medial division of the left nst. very small (100-200 micron diameter) iontophoretic injections of horser ... | 1983 | 6363505 |
| the location of chronotropic cardioinhibitory vagal motoneurons in the medulla of the rabbit. | vagal preganglionic motoneurons originating in nucleus ambiguus (na) and dorsal vagal nucleus (dvn) were identified via retrograde labeling with horseradish peroxidase (hrp). dvn and na were then explored for cardiovascular responsive sites using microstimulation. stimulation within dvn from slightly caudal to obex to 3.00 mm rostral to obex produced a primary bradycardia (n = 15, x = -123 bpm). stimulation within na from slightly rostral to obex to 1.5 mm caudal to obex produced a similar prima ... | 1983 | 6363506 |
| transitory macrophages in the white matter of the developing visual cortex. i. light and electron microscopic characteristics and distribution. | injections of horseradish peroxidase (hrp) into the occipital cortex of the kitten and diffusing to the white matter label a widely distributed microglial population and in addition, cells with light and electron microscopic features of 'gitter cells'. the latter are concentrated in a complex and highly consistent system of interconnected clusters in the white matter of the lateral, postlateral, middle suprasylvian and posterior ectosylvian gyri, as well as on the roof of the lateral ventricle. ... | 1983 | 6198051 |
| vagal mechanisms controlling serotonin release from the gastrointestinal tract and pyloric motor function. | this chapter focuses on investigations of two mechanisms controlled by the vagal nerve; serotonin (5-ht) release from the small intestine and pyloric motor function. morphological, physiological, pharmacological and biochemical methods were combined in these studies. 5-ht is mainly stored in enterochromaffin cells (ec), but is present also in mast cells and nerve terminals of the gut, as observed by immunocytochemistry. vagal nerve stimulation causes a release of 5-ht from ec to the portal circu ... | 1983 | 6198349 |
| enzyme cytochemistry of blood-brain barrier (bbb) disturbances. | alkaline phosphatase (ap) is one of the enzymes which is highly active in the plasmalemma of endothelial cells (ecs) of bbb-type microvessels. in the ecs of non-bbb type vessels, the reaction for ap (and other phosphatases) is negative (e.g. choroid plexus, area postrema, hypophysis). after bbb damage, the leakage of the vessels can be demonstrated by the use of horseradish peroxidase (hrp). concomitantly, changes in polar distribution of ap in the ecs occur, paralleled by the appearance of nume ... | 1983 | 6306982 |
| ultrastructural localization of the transferrin receptor and transferrin on marrow cell surfaces. | the monoclonal antibody okt9 (a known transferrin receptor antibody) and a monoclonal antibody to transferrin (atfn) were used to localize the transferrin receptor and transferrin on marrow cells. after incubation of cell suspensions with the antibody, the cells were reacted with an affinity purified fab fragment of goat anti-mouse igg conjugated to horseradish peroxidase (gam-hrp), which was in turn visualized by reaction with 3,3'-diaminobenzidine (dab). erythroblast cell surfaces stained inte ... | 1983 | 6307335 |
| paralemniscal reticular formation: response of cells to a noxious stimulus. | extracellular single unit recordings were made in the paralemniscal reticular formation in adult male rats. a majority of the cells studied were characterized as nociceptive because a noxious stimulus evoked a change (either an increase or decrease) in their spontaneous firing rates. norepinephrine (ne) administered microiontophoretically usually mimicked the response to the noxious stimulus (foot pinch). after a neuron had been characterized with respect to its response to ne and the noxious st ... | 1983 | 6307465 |
| cellular localization of five structural proteins of sendai virus studied with peroxidase-labelled fab fragments of monoclonal antibodies. | by the use of horseradish peroxidase-labelled fab fragments of monoclonal antibodies, five major structural components of sendai virus, namely the nucleocapsid (np), polymerase (p), matrix (m), fusion (f), and haemagglutinin-neuraminidase (hn) proteins were localized in infected vero cells. the p and np proteins were found in association with large clusters of ribosome-like particles and nucleocapsids in the cell cytoplasm. they were not concentrated at the cytoplasmic membrane, except in nucleo ... | 1983 | 6308131 |
| synaptic interactions between mammalian central neurons in cell culture. iii. morphophysiological correlates of quantal synaptic transmission. | the statistical properties of excitatory synaptic transmission between neurons in cell cultures of fetal mouse spinal cord were studied and the anatomical extent of these connections demonstrated by horseradish peroxidase (hrp) injection of the presynaptic cell. in conjunction with previous experiments (7), we have correlated the number of boutons involved in a given synaptic connection with the physiologically determined number of release elements. the number of boutons is somewhat greater than ... | 1983 | 6308178 |
| anatomical and electrophysiological studies on the reciprocal projections between the subthalamic nucleus and nucleus tegmenti pedunculopontinus in the rat. | reciprocal projections between the subthalamic nucleus of the diencephalon and the brain stem nucleus tegmenti pedunculopontinus have been demonstrated and studied anatomically and electrophysiologically in the rat. injections of the fluorescent compound fast blue into pedunculopontinus and the surrounding peribrachial region of the mesencephalon gave rise to retrogradely-labelled neurone cell bodies in the ipsilateral subthalamic nucleus. a minimum of 1% of subthalamic nucleus neurones appeared ... | 1983 | 6308507 |
| transient retinal fibers to the inferior colliculus in the newborn albino rat. | transient retinal fibers to the inferior colliculus were found in newborn albino rats. after horseradish peroxidase injection into the vitreous cavity of rats of 1-3 days of age, anterogradely-labeled retinal fibers were traced through the superior colliculus to the inferior colliculus. these retinal fibers to the inferior colliculus were not seen in rats older than 7 days of age. | 1983 | 6308520 |
| role of myeloperoxidase in the luminol-dependent chemiluminescence response of phagocytosing human monocytes. | the luminol-dependent chemiluminescence (cl)-response of phagocytosing declined steadily during in vitro differentiation and was approximately 10% of the initial value by the fourth day of culture. a parallel decline in myeloperoxidase (mpo)-activity of monocyte cell lysates was observed during the same period, and a close correlation was found between peak luminol-dependent cl-response and mpo-activity. the lucigenin-dependent cl-response of phagocytosing monocytes in parallel cultures declined ... | 1983 | 6308951 |
| the oxidative burst and related phenomena in mouse macrophages elicited by different sterile inflammatory stimuli. | mouse peritoneal macrophages (mpm) elicited by paraffin oil (po) or thioglycollate (tg) were compared in their capacity to generate oxidative burst (ob) products such as superoxide ion (o2-) and hydrogen peroxide (h2o2) and lyse target erythrocytes. strong ob stimulants such as 12-o-tetradecanoyl-phorbol-13-acetate (tpa), opsonized zymosan; and wheat-germ agglutinin (wga) were found to stimulate o2 and h2o2 production by both po- and tg-elicited mpm. po-elicited mpm exhibited a vigorous ob respo ... | 1983 | 6309652 |
| slow internalization of human chorionic gonadotropin by cultured granulosa cells. | kinetic studies were performed on two-day cultures of rat ovarian granulosa cells to follow the fate of surface-bound 125i-labeled human chorionic gonadotropin (125i-hcg). low ph was used to release hcg from its surface receptor, allowing us to distinguish between surface-bound and internalized hormone. because our results indicated that hormone is lost from the cell surface by dissociation as well as internalization, equations were derived to determine independent rate constants for each proces ... | 1983 | 6311848 |
| retrograde labeling of striato-nigral neurons in the rat by three different tracers. | the three tracers horseradish peroxidase (hrp), 4',6-diamidino-2-phenylindol-2hcl (dapi) and fast blue (fb) differ in retrograde labeling of striato-nigral neurons. after a 24 h survival, injection of dapi into the ventral tegmentum labeled numerous cells throughout the neostriatum, whereas an identical amount of hrp only labeled cells in circumscribed areas of the neostriatum. injections of fb labeled a substantial number of neostriatal neurons after a survival time of 4 days, but not after 24 ... | 1983 | 6312380 |
| the terminations of the spinothalamic tract in the cat. | the termination sites of the spinothalamic tract (stt) in the cat have been examined using the anterograde transport of wheat germ agglutinin conjugated to horseradish peroxidase (wga-hrp). cats which received an injection of wga-hrp at the spinal cervical level (c7-c8) displayed anterograde labelling in the ventral posterior lateral (vpl), ventrolateral, lateral posterior, limitans, suprageniculate, medial geniculate pars magnocellularis, central lateral, paracentral, centrum medianum, submediu ... | 1983 | 6312381 |
| neuronal organization in the dorsal horn of the spinal cord. | two major output systems from the dorsal horn, the spinocervical tract (sct) and the post-synaptic dorsal column (psdc) pathway, have been characterized by electrophysiological methods and the injection of horseradish peroxidase into single neurones. neurones of both systems have somata in laminae iii, iv and v but they differ in their physiology and anatomy. sct neurones have few inputs and their receptive fields are simple. psdc neurones show several patterns of excitatory convergent input and ... | 1983 | 6312776 |
| the effect of monocular deprivation on cells in the c-laminae of the cat lateral geniculate nucleus. | five cats monocularly deprived by lid suture between 2 and 3 weeks of age were used experimentally between the ages of 16 and 52 weeks. an intraocular injection of horseradish peroxidase was used to reveal the individual c laminae (c, c1, c2) in the dorsal lateral geniculate nucleus. soma size measurements of cells in the c laminae showed significant differences between cells in the deprived layers c and c1 and the corresponding nondeprived layers, but not between cells in deprived and nondepriv ... | 1983 | 6313135 |
| retinotopic scatter of optic tract fibres in the cat. | the retinal fibers in the optic tract (ot) of the cat have a roughly retinotopic arrangement. the retinotopic scatter of ot fibres at the rostral pole of the superior colliculus (sc) and at the lateral margin of the lateral geniculate nucleus (lgn) was investigated using the retrograde transport of horseradish peroxidase. small lesions and injections were made in the tract and from the distribution of labelled ganglion cells in the retina the retinotopic precision was measured. at an eccentricit ... | 1983 | 6313417 |
| demonstration of spinocerebellar projections in cat using anterograde transport of wga-hrp, with some observations on spinomesencephalic and spinothalamic projections. | wheat germ agglutinin-horseradish peroxidase (wga-hrp) conjugate was injected into the lumbar or cervical spinal cord of adult cats in order to examine its usefulness as an anterograde tracer of long ascending spinal tracts. heavily labeled fibers and terminal-like structures were found in restricted regions of the granular layer of the cerebellar cortex in all animals. the terminal-like structures showed features characteristic of mossy fiber rosettes. many labeled terminals were found in regio ... | 1983 | 6313420 |
| the mechanism of action of lymphokines. vii. modulation of the action of macrophage migration inhibitory factor by antioxidants and drugs affecting thromboxane synthesis. | guinea pig peritoneal exudate macrophages are active producers of oxygen radicals in response to membrane stimulation. we examined the involvement of oxygen radicals in the inhibition of macrophage migration caused by the lymphokine, macrophage migration inhibitory factor (mif). it was found that the presence of scavengers of superoxide and hydrogen peroxide did not influence mif action. exogenous horseradish peroxidase, however, significantly enhanced the migration inhibitory effect of mif. amo ... | 1983 | 6313550 |
| enzyme-linked immunosorbent assay for the detection of human rotavirus in stools. | a simple method for the detection of human rotavirus in stools is described, using a double antibody sandwich enzyme-linked immunosorbent assay. polysterene microtitre plates were used as solid phase. four capture antibodies were tried, bovine, egg-derived, guinea pig and monoclonal antibody to rotavirus. both bovine and egg-derived antirotavirus labelled with horseradish peroxidase were used as the detecting antibodies. the results obtained were compared with a commercially available elisa, rot ... | 1983 | 6313726 |
| corticotectal and corticothalamic efferent projections of siv somatosensory cortex in cat. | substantial corticotectal (and corticothalamic) projections from the cortex of the anterior ectosylvian sulcus (aes) were demonstrated in the cat using the axonal transport methods of autoradiography and horseradish peroxidase. the corticotectal projection arises nearly exclusively from medium-large pyramidal cells in lamina v. one of the densest projecting areas of the aes is the rostral aspect of its superior bank, where a fourth somatotopic representation (siv) has recently been demonstrated. ... | 1983 | 6313872 |
| ascending projections to the medial geniculate body of the cat: evidence for multiple, parallel auditory pathways through thalamus. | the sources of ascending input to the medial geniculate body (mgb) of the cat were studied using the retrograde transport of horseradish peroxidase (hrp). hrp injections were made iontophoretically through micropipettes which were also used to record physiological properties at the injection sites. this technique produced small injections which appeared to be restricted to single subnuclei. the tectothalamic projection of the auditory system was found to consist of at least four distinct and sep ... | 1983 | 6313877 |
| quantitation of intracellular membrane-bound enzymes and receptors in digitonin-permeabilized cells. | the distribution of membrane-bound receptors and enzymes between the cell surface and the cell interior can be determined without solubilization or gross disruption of cell organelles in the presence of the nonionic detergent digitonin. this steroid glycoside permeabilizes cells, releases cytoplasmic proteins with subunit molecular weights up to 200,000, and allows exogenous molecules to gain access to intracellular receptors. all cell types examined were affected similarly by digitonin. permeab ... | 1983 | 6314844 |
| spinal afferents to the ventrobasal thalamic complex in the rat: an anatomical study using wheat-germ agglutinin conjugated to horseradish peroxidase. | the anterograde axonal transport of wheat-germ agglutinin conjugated to horseradish peroxidase was used to reassess the spinal afferents to the ventrobasal complex in the rat. somatotopically-organized labeled fibers and fiber terminals could be observed in the lateral portion of the complex. the distribution of the labeling corresponded to that previously described for afferents originating from the dorsal column nuclei. terminal fibers were drawn and their morphology analyzed in comparison to ... | 1983 | 6315154 |
| studies on in vitro chrysotile-pleural mesothelial cell interaction: morphological aspects and metabolism of benzo-3,4-pyrene. | cultures of rat pleural mesothelial cells (pmc) were exposed to nonlethal doses of uicc chrysotile a. the morphology was studied by optical and electron microscopy. the consequences of chrysotile ingestion on the rate of pinocytosis of horseradish peroxidase (hpr) metabolism and benzo-3-4-pyrene (bp) were studied. nonlethal doses of chrysotile (5 micrograms/ml) induced a time-dependent vacuolation of pmc; a dose-dependent inhibition of the vacuolation was observed when pmc were pretreated with d ... | 1983 | 6315357 |
| subcortical projections to the occipital and parietal lobes of the chimpanzee brain. | the subcortical sources of afferents to occipital and parietal cortex were studied in two chimpanzees with the aid of retrogradely transported horseradish peroxidase (hrp). in chimpanzee 1, hrp was injected into right cortical areas 17 and 18; chimpanzee 2 received hrp into right areas 17, 18, 19, and 39. the following subcortical structures were found to project to area 17 and/or area 18: locus coeruleus, dorsal raphe nucleus, nucleus annularis, nucleus centralis superior, pontine reticular for ... | 1983 | 6315779 |
| columnar aggregation of prefrontal and anterior cingulate cortical cells projecting to the thalamic mediodorsal nucleus in the monkey. | the columnar arrangement of the prefrontal and anterior cingulate cortex cells that project to the thalamic mediodorsal (md) nucleus in rhesus and formosan monkeys was studied by injecting horseradish peroxidase into the md nucleus. labelled cells appeared ipsilaterally in layers v and vi in the dorsolateral, medial, and orbital prefrontal cortex, and in layer vi in the anterior cingulate cortex. labelled cells were aligned in the radially oriented cords of neurons and grouped into clusters, 25- ... | 1983 | 6315780 |
| the development and postnatal organization of motor nuclei in the rat thoracic spinal cord. | the topographic organization of the motor nuclei supplying individual thoracic muscles in postnatal rats was investigated by horseradish peroxidase (hrp) labelling from the periphery. to determine whether aspects of the same organization are apparent at early developmental stages, we used hrp to label neurons contributing axons to the two primary rami of thoracic nerves in rat fetuses ranging in age from the 13th day of gestation to birth. this developmental period includes stages during which p ... | 1983 | 6315781 |
| afferent projections to the oral motor nuclei in the rat. | projections to the trigeminal, facial, ambiguus, and hypoglossal motor nuclei were determined by using horseradish peroxidase histochemistry. most of the afferent projections to these motor nuclei were from the brainstem reticular formation, frequently in areas adjacent to other synergetic motor nuclei. the reticular formation lateral to the hypoglossal nucleus and reticular structures surrounding the trigeminal motor nucleus projected to each of these other brainstem motor nuclei involved in or ... | 1983 | 6315785 |
| principles of organization of the corticopontocerebellar projection to crus ii in the cat with particular reference to the parietal cortical areas. | in 13 cats injections of horseradish peroxidase-wheat germ agglutinin in various parts of the cerebral cortex were combined with injections in the cerebellar crus ii in the same animal in order to study the cortical regions that may influence the crus ii via the pontine nuclei. in 2 cats lesions in the cerebral cortex were combined with horseradish peroxidase injections in the crus ii. in the pons terminal regions (anterogradely labelled from the cerebral cortex or containing terminal degenerati ... | 1983 | 6316199 |
| the septohippocampal projection in the rat: an electron microscopic horseradish peroxidase study. | in order to identify the specific targets of the septohippocampal projection in the rat, horseradish peroxidase localization at the electron microscopic level was used. following injections of free horseradish peroxidase into the medial septum, sections of the dorsal hippocampal formation were reacted with diaminobenzidine and processed for electron microscopy by routine methods. sections were viewed unstained. horseradish peroxidase labeling in the dentate gyrus was predominantly in the supra- ... | 1983 | 6316200 |
| long collateral branches of substantia nigra pars reticulata axons to thalamus, superior colliculus and reticular formation in monkey and cat. multiple retrograde neuronal labeling with fluorescent dyes. | in order to gain some impressions about the degree to which individual neurons of the pars reticulata of the substantia nigra send long collateral branches to more than one of its three major targets (thalamus, superior colliculus, reticular formation), two, or all three targets were injected with fluorescent dyes (evan's blue, granular blue, nuclear yellow, propidium iodide) in six squirrel monkeys and four cats. the best results were obtained in the monkey brain with injections of evan's blue ... | 1983 | 6316201 |
| degenerative and regenerative changes in central projections of glossopharyngeal and vagal sensory neurons after peripheral axotomy in cats: a structural basis for central reorganization of arterial chemoreflex pathways. | hypoxic hyperventilation in cats is a reflex normally initiated by afferent impulses originating in the carotid body and conducted to the brain stem by the carotid sinus nerves. the reflex response is abolished acutely after section of carotid sinus nerves and excision of the carotid bodies; but, chronically, there is a chemoreflex restoration which is mediated by the aortic body via the aortic depressor nerves. the restoration is associated temporally with changes in efficacy of ventilatory ref ... | 1983 | 6316202 |
| cerebellar cortical afferents from the periaqueductal grey in the cat. | a cerebellar afferent connection from the periaqueductal grey has been demonstrated in the cat by means of retrograde transport of the wheat germ agglutinin-horseradish peroxidase complex. the projection is bilateral. its main origin is the ventrocaudal part of the periaqueductal grey, and the fibres reach different cerebellar cortical regions. | 1983 | 6316217 |
| evidence for thalamic projections from external cuneate nucleus, cell groups z and x, and the mesencephalic nucleus of the trigeminal nerve in squirrel monkey. | horseradish peroxidase (hrp) was injected into the thalamic ventral tier of squirrel monkey and the brainstem was examined for the presence of hrp-reactive cells in the external cuneate nucleus (ecn), nuclei z and x, and the mesencephalic nucleus of the trigeminal nerve (mnv). results indicate that the ecn, z and x have a direct projection to contralateral ventral tier. thalamic projecting cells in the ecn are located predominantly in the medial part of this nucleus. the mnv projects bilaterally ... | 1983 | 6316220 |
| surface localization of wheat germ agglutinin and concanavalin a binding sites on normal human blood cells: an ultrastructural histochemical study. | in order to determine the extent and variations in surface concavanalin a (con a) and wheat germ agglutinin (wga) labeling of different varieties of normal blood cells, gluraraldehyde-fixed human blood cells were exposed to con a-gold labeled horseradish peroxidase (con a-hrp-g) and wga-gold labeled ovomucoid (wga-ovo-g) histochemical methods. the resultant particulate reaction product permitted assessment of binding and number of gold particles per micrometer of cell surface. particle counts an ... | 1983 | 6316580 |
| localization of concanavalin a binding sites in human pituitary adenoma cells as revealed by hrp-labelling method. | intracellular lectin (con a)-binding sites of human pituitary adenoma were examined by electron microscopy using the horseradish-peroxidase (hrp)-labelling technique. in this study were used 16 cases of human pituitary adenomas operated on in our clinics between 1977 and 1981: they concluded 4 each of prl-, gh-, acth-producing and hormonal non-functioning adenomas. in parallel with the detection of lectin-binding sites, basal levels of their secreting hormones were determined by the radioimmunoa ... | 1983 | 6318506 |
| "peroxidatic" form of cytochrome oxidase as studied by x-ray absorption spectroscopy. | x-ray absorption spectroscopy shows pulsed oxidase to be similar to resting oxidase but to lack the sulfur bridge between iron and copper of active sites (powers, l., y. ching, b. chance, and b. muhoberac, 1982, biophys. j., 37[2, pt. 2]: 403a. [abstr.] ) the first shell ligands and bond lengths of the pulsed oxidase active site heme most clearly fit the ferric peroxidases from horseradish and yeast, and the pulsed oxidase cyanide compound resembles the low spin hemoprotein cyanide compounds. th ... | 1983 | 6318841 |
| uptake of [3h]taurine into medium-size neurons and into identified striatonigral neurons in the rat neostriatum. | injection of [3h]taurine into the neostriatum of the rat led to accumulation of radioactivity in a distinct population of neurons. taurine-accumulating neurons had a medium-size cell body, a smooth nucleus and relatively little cytoplasm. other neuron types, e.g. large neurons with indented nuclei, did not accumulate taurine. striatonigral neurons, identified by retrograde transport of horseradish peroxidase, were found to take up [3h]taurine. it is suggested that taurine-accumulating neurons in ... | 1983 | 6318902 |
| organization of the efferent projections of the medial superior olivary nucleus in the cat as revealed by hrp and autoradiographic tracing methods. | features of the organization of the efferent axonal projections from the medial superior olivary nucleus (mso) in the cat were studied. in order to determine the origin and distribution of projections from mso, the retrograde horseradish peroxidase (hrp) and autoradiographic tracing methods were used. the results showed that (1) in both hrp and autoradiographic studies the projection to the inferior colliculus was largely ipsilateral, although a contralateral component was present; (2) the proje ... | 1983 | 6319460 |
| direct projections from vestibular nuclei to facial nucleus in cats. | postsynaptic potentials were recorded from motoneurons in the facial nucleus in response to stimulation of the vestibular and trigeminal nerves. the motoneurons were identified by antidromic activation from their peripheral axons. disynaptic excitatory and inhibitory postsynaptic potentials (epsps and ipsps) and mixed epsp/ipsps were recorded in response to vestibular nerve stimulation, ranging in latency from 0.9 to 2.1 ms, with most at 1.5 ms. activity in secondary vestibular axons recorded wi ... | 1983 | 6319618 |
| central cholinergic pathways in the rat: an overview based on an alternative nomenclature (ch1-ch6). | monoclonal antibodies to choline acetyltransferase and a histochemical method for the concurrent demonstration of acetylcholinesterase and horseradish peroxidase were used to investigate the organization of ascending cholinergic pathways in the central nervous system of the rat. the cortical mantle, the amygdaloid complex, the hippocampal formation, the olfactory bulb and the thalamic nuclei receive their cholinergic innervation principally, from cholinergic projection neurons of the basal foreb ... | 1983 | 6320048 |
| ultrastructural and electrophysiological properties of accessory abducens nucleus motoneurones: an intracellular horseradish peroxidase study in the cat. | the physiological and morphological (light and electron microscopy) properties of six retractor bulbi motoneurones were analysed using the technique of intracellular recording and intracellular labelling with horseradish peroxidase. the retractor bulbi motoneurones were identified by antidromic invasion and orthodromic responses following stimulation of trigeminal afferents were studied. two of these motoneurones were examined ultrastructurally. terminal boutons forming synapses with labelled so ... | 1983 | 6320049 |
| the retinal location and fate of ganglion cells which project to the ipsilateral superior colliculus in neonatal albino and hooded rats. | we have studied the organization of the ipsilateral retinocollicular pathway in neonatal rats by injecting the enzyme horseradish peroxidase (hrp) into the superior colliculus within 24 h of birth and later examining the location of labelled cells in the contralateral and ipsilateral retinae. one day after hrp injection, regardless of the location of the injection site in the superior colliculus, the great majority (over 80%) of ipsilaterally projecting cells was located in the lower peripheral ... | 1983 | 6320062 |
| the distribution of afferent fibers from the gastrocnemius-soleus muscle in the dorsal horn of the cat, as revealed by the transport of horseradish peroxidase. | the transganglionic transport of horseradish peroxidase (hrp) was used to examine the distribution of afferent fibers from the gastrocnemius-soleus (gs) muscle in the dorsal horn of the cat. intense labeling was consistently observed in lamina i (in segments l4 to s3) and in the lateral portion of lamina v (segments l6 and s1-3), but not to any significant extent in laminae ii-iv. these terminal fields were ascribed to the small-diameter (group iii/iv) gs afferent fibers. | 1983 | 6320063 |
| an anatomical investigation of visual inputs to the cat orbital cortex. | the orbital region of the cortex in the cat is known to be involved in multimodal sensory functions. visual afferent connections of this area have been investigated using the horseradish peroxidase technique. our results demonstrate the existence of projections from ipsilateral thalamic nuclei essentially at the suprageniculate and magnocellular medial geniculate junction, well known to have multimodal physiological properties. | 1983 | 6199694 |
| electron microscopic observations on the synaptic contacts of group ia muscle spindle afferents in the cat lumbosacral spinal cord. | after intra-axonal injection of horseradish peroxidase (hrp) into afferent fibers originating from muscle spindle primary endings of the cat gastrocnemius, group ia boutons located in the ventral horn of the spinal cord were identified and studied electron microscopically. the ia boutons were invariably found to contain spherical synaptic vesicles (s-type boutons), and a number of them were also postsynaptic to smaller p-type boutons (large s-type boutons with axo-axonic contacts). none of the p ... | 1983 | 6221776 |
| posterior and anterior permeability defects? morphologic observations on streptozotocin-treated rats. | structural abnormalities of the blood-ocular barrier were examined in streptozotocin (stz)-treated hyperglycemic rats, after 9 days, 6 months, and 10 months' duration of "diabetes," and in normoglycemic control animals using the horseradish peroxidase tracer technique combined with light and electron microscopy. the most frequent abnormalities consisted of small areas of diffuse dense staining by the tracer of (1) the retinal pigment epithelium and (2) the nonpigmented ciliary epithelium. pigmen ... | 1983 | 6224756 |
| ultrastructural demonstration of fc gamma-receptors in sarcoid skin lesions. | immune complexes of horseradish peroxidase oxidase (hrp) and rabbit igg antibodies to hrp were used to study the fc gamma-receptors in granulomas of cutaneous sarcoidosis. cryostat sections of skin biopsies were incubated with hrp-anti-hrp, and the peroxidase activity was demonstrated with 3,3-diaminobenzidine tetrahydrochloride and h2o2. most cells in the granulomas, both epithelioid and giant cells, were stained. by electron microscopy, the reaction products were localized to the plasma membra ... | 1983 | 6225401 |
| epidermal cell proliferation following an active arthus reaction in the guinea pig. | active arthus reactions were provoked by injections of 100 micrograms horseradish peroxidase (hrp), 10 micrograms hrp and 100 micrograms bovine serum albumin (bsa) into the skin of sensitized guinea pigs. labeling indices (li) of epidermal basal cells were measured 1, 4, 8, 24, 48 and 72 h later by the in vivo 3h-thymidine labeling technique, and compared with those obtained with injections of antigens into the skin of non-sensitized guinea pigs. from 1-8 h after the induction of an active arthu ... | 1983 | 6229219 |
| observations on the projection from the perihypoglossal nuclei onto the cerebellum in the macaque monkey. | the occurrence and distribution of retrogradely labeled cells in the perihypoglossal nuclei of the monkey were mapped after injections of horseradish peroxidase in various cerebellar cortical regions. in general the findings are in accord with those made in the cat. the flocculus receives a heavy bilateral projection from the nucleus prepositus, particularly from its caudoventral part, and from the nucleus of roller. there is an apparently scanty projection from the nucleus intercalatus. the uvu ... | 1983 | 6198996 |
| the relationship of microvesicles to the plasmalemma of rat adipocytes. | ruthenium red binding demonstrates that the extensive microvesicle system of isolated rat adipocytes is, for the most part, open to and continuous with the plasmalemma proper. morphometric estimates indicate that insulin treatment has no effect on the relationship between microvesicles and the cell surface. neither does insulin affect the apparent lack of pinocytotic activity of these vesicles as judged by a time course analysis of cells incubated with horseradish peroxidase, which is bound to t ... | 1983 | 6199205 |
| direct projections to the pretectum and the midbrain reticular formation from auditory relay nuclei in the lower brainstem of the cat. | direct projections to the pretectum and the midbrain reticular formation from auditory relay nuclei in the lower brainstem were examined by the retrograde and anterograde tracer methods in the cat. after horseradish peroxidase (hrp) injection into the pretectomesencephalic reticular region (pt-mrf), which includes caudoventral regions of the pretectum and rostrodorsal regions of the midbrain reticular formation, labeled neurons were seen in the dorsal nucleus of the lateral lemniscus (dll), the ... | 1983 | 6198023 |
| afferent connections of the median preoptic nucleus in the rat: anatomical evidence for a cardiovascular integrative mechanism in the anteroventral third ventricular (av3v) region. | the afferent connections of cell groups in the anteroventral third ventricular (av3v) region were investigated using wheat germ agglutinin-horseradish peroxidase conjugate injections into the av3v area. the median preoptic nucleus was found to receive a unique set of inputs from the subfornical organ, the paraventricular nucleus of the hypothalamus, the parabrachial nucleus, the nucleus of the solitary tract and the ventrolateral medulla. as each of these structures has been implicated in variou ... | 1983 | 6198025 |
| ultrastructure of muscle spindle afferent terminations in lamina vi of the cat spinal cord. | two group ia muscle spindle afferents were impaled in the lumbosacral enlargement of the cat's spinal cord and intra-axonally labeled with horseradish peroxidase. terminations in lamina vi were examined with the electron microscope. boutons formed synaptic associations with somata and large dendritic shafts in the lamina vi neuropile and received axo-axonic contacts. it is concluded that group ia terminals synapse with the proximal regions of lamina vi neurons and are under strong presynaptic co ... | 1983 | 6198026 |
| organization of projections from the superior colliculus to the thalamic lateral posterior nucleus in the rat. | injections of horseradish peroxidase into the lateral posterior nucleus (lp) of the rat thalamus resulted in retrograde labeling of neurons in the superior colliculus (sc). approximately 80% of the labeled cells occurred in the stratum opticum and 20% in the stratum griseum superficiale. the system of projections is organized such that medial parts of sc project to dorsal lp and lateral parts of sc project to ventral lp. | 1983 | 6198028 |
| cerebellar nuclear afferents from the lateral reticular nucleus in the cat. | cerebellar afferent fibers were anterogradely stained throughout their length after injections of horseradish peroxidase labeled wheat germ agglutinin in the lateral reticular nucleus in cats. most fibers ascended through the ipsilateral restiform body, but a few fibers ran on the contralateral side. some anterogradely stained fibers crossed the midline in cerebellum. terminal fibers were present in the granular layer and in the cerebellar nuclei. plexuses of nuclear terminal fibers were found i ... | 1983 | 6198029 |
| ultrastructural observations on endothelial cell vesicles after horseradish peroxidase microinjection into blood and dextran perfused microvessels of rat mesentery. | horseradish peroxidase (hrp) was injected for 5 sec into the arteriole that supplies the mesocecum in rats. this microperfusion was followed by fixation (with arrest of blood flow in 203 sec) either immediately or after an interval of 10 sec, 20 sec, 60 sec, or 120 sec and a diaminobenzidine cytochemical processing of the tissue was performed. the distribution of hrp was determined in the electron microscope. after immediate fixation hrp labelled 93% of luminal endothelial vesicles, 45% of cytop ... | 1983 | 6206008 |
| intratypic differentiation of poliovirus strains by enzyme-linked immunosorbent assay (elisa): poliovirus type 1. | a double antibody sandwich-elisa has been developed for the detection of antigenic differences between wild and vaccine derived strains of poliovirus type 1. poliovirus strains antibodies were prepared in rabbits by immunization with virus suspensions of: sabin lsc2ab (vaccine derived) and brunhilde and mahoney (wild types). igg fractions were purified from antiserum by precipitation with ammonium sulphate and deae-sephadex a50 chromatography. purified igg antibodies were used for coating of mic ... | 1983 | 6203794 |
| morphological and electrophysiological properties of trigeminal neurones projecting to the accessory abducens nucleus of the cat. | horseradish peroxidase was injected into the somata or axons of neurones located in the nucleus oralis of the spinal trigeminal complex and projecting to the accessory abducens nucleus. a group of 43 axons with electrophysiologically identified responses to the stimulation of three different areas of the face were studied. the latencies of their orthodromic responses following trigeminal stimulation and their pattern of discharges were compared to those of secondary trigeminal neurones and retra ... | 1983 | 6201377 |
| [distribution of cell bodies of the sympathetic preganglionic fibers to the stellate ganglion in the monkey: a study using horseradish peroxidase technic]. | 1983 | 6201423 | |
| tracer uptake by photoreceptor synaptic terminals. i. dark-mediated effects. | this electron microscope study examines the sites of uptake and subsequent redistribution of the extracellularly applied tracer, horseradish peroxidase (hrp), in photoreceptor synaptic terminals during dark adaptation. first, chicks are sacrificed by perfusion fixation at various intervals after the addition of tracer to in situ eyecups. it is demonstrated that when terminals contain only one tracer-labeled organelle, this may be either a synaptic or a coated vesicle. at later times vacuoles in ... | 1983 | 6197533 |
| distribution of glycoconjugates in the kidney studied by use of labeled lectins. | distribution of glycoconjugates in different areas of the rat kidney was studied by light and electron microscopy using six different horseradish peroxidase-labeled lectins. glomeruli and brush borders of the proximal tubules reacted differently to these lectins, which indicated differences in the carbohydrate compositions of those regions. the ascending limb of henle's loop (alh) had strong binding sites for peanut agglutinin (pna) and soybean agglutinin (sba). dolichos biflorus agglutinin (dba ... | 1983 | 6186720 |
| light microscopic histochemical detection of sugar residues in secretory glycoproteins of rodent and human tracheal glands with lectin-horseradish peroxidase conjugates and the galactose oxidase-schiff sequence. | lectins conjugated to horseradish peroxidase were used to stain paraffin sections of mouse, rat, and human respiratory tract tissues. an additional method was applied utilizing galactose oxidase to oxidize the c-6 hydroxyl of galactose and n-acetylgalactosamine residues and the resulting aldehyde was visualized with 2% schiff's reagent. sections were stained prior to and after removal of sialic acid residues. oligosaccharides with terminal beta-galactose and alpha-n-acetylgalactosamine residues ... | 1983 | 6186733 |
| distal infrapyramidal and longitudinal mossy fibers at a midtemporal hippocampal level. | timm's and horseradish peroxidase histochemical methods were used to demonstrate a small, distal infrapyramidal mossy fiber projection at midtemporal hippocampal levels in the rat. the timm's sulfide silver method revealed sparse granules of dark, mossy fiber-like staining at an infrapyramidal location in an area roughly equivalent to hippocampal subfield ca3a (close to the ca1 border). the use of anterograde transport of horseradish peroxidase enabled the visualization of short mossy fiber axon ... | 1983 | 6186343 |
| morphological alterations of retinal cells accompanying inhibition of axonal transport by kainic acid. | the effect of kainic acid (ka) on rat retinal morphology was investigated with emphasis on the ganglion cell layer. two hours after intraocular injection of ka, 70% of the neurones in the ganglion cell layer display morphological alterations. these alterations at the ultrastructural level include the absence of identifiable golgi complexes and the presence of abundant round empty vacuoles among other nuclear and cytoplasmic modifications. these alterations of the golgi complex are discussed in r ... | 1983 | 6186510 |