Publications

identification and characterization of a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis.	a putative serine protease expressed in vivo by mycobacterium avium subsp. paratuberculosis was isolated from a lambda gt11 genomic expression library by screening with serum from a naturally infected sheep. the gene was contained in two overlapping clones, which were shown by antibody elution to encode a protein of 34 kda in m. a. paratuberculosis. the clones were sequenced and database searches detected a motif identical to the active serine site in trypsin, and 30% homology to the putative se ...	1994	7921248
[cat scratch disease].	cat scratch disease (csd) is usually manifested in children and young adults as a benign, chronic self-limited lymphadenopathy. however, various atypical presentations have been described, especially in immunocompromised hosts. 1 of 5 cases of csd diagnosed here during 1985-1993 is presented. in 3 cases the diagnosis was supported by lymph node biopsy in which the organisms were visualized by warthin-starry silver impregnation staining. recent publications indicate that afipia felis and rochalim ...	1994	7927015
[recognition and identification of unknown infectious agents].	the recognition and the identification of previously unrecognized infectious agents require a multidisciplinary approach to specify the nosologic entity of the disease and the epidemiological data, especially the modes of transmission and the risk factors, as well as to discover the microorganism in the laboratory. in the past 20 years, significant breakthroughs have been achieved in cellular cultures (growth factors), in immunology (monoclonal antibodies), and moreover in molecular biology, whi ...	1994	7939294
[cat-scratch disease and bacillary angiomatosis. an old and a new infectious disease with common etiology?].	a review of cat-scratch disease (csd) and bacillary angiomatosis (ba) is presented on the basis of published articles. two newly identified bacteria--rochalimaea henselae and afipia felis--have been isolated from patients with csd. preliminary investigations seem to indicate that a. felis is an uncommon cause of the disease. csd may appear as a local suppurative lymphadenopathy or a systemic infection. ba is caused by rochalimaea species and may appear as cutaneous, mucous or visceral angiomas o ...	1994	7998352
detection of rochalimaea henselae dna in specimens from cat scratch disease patients by pcr.	a pcr assay was developed by using degenerate primers that allow amplification of a 414-bp fragment of dna from the rickettsia-like organisms rochalimaea henselae and r. quintana. internal oligonucleotides were used as hybridization probes, permitting rapid differentiation of these two rochalimaea species. dnas from 12 different isolates of r. henselae were amplified with the pcr primers, and the resulting 414-bp pcr product hybridized only with the r. henselae-specific probe. dnas from four dif ...	1994	8027347
rochalimaea antibodies in hiv-associated neurologic disease.	rochalimaea henselae, a recently described pathogen thought to cause syndromes as varied as bacillary angiomatosis, parenchymal bacillary peliosis, fever with bacteremia, and cat-scratch disease, is associated with cns diseases including cerebral and retinal bacillary angiomatosis, as well as cat-scratch-related encephalitis, myelitis, cerebral arteritis, and retinitis. we used a newly developed enzyme immunoassay and the polymerase chain reaction to investigate the association of r henselae inf ...	1994	8035937
ophthalmic manifestations of rochalimaea species.	rochalimaea henselae and r. quintana belong to the order rickettsiales and are thought to be responsible for trench fever, bacillary angiomatosis, and cat scratch disease. we recently examined four patients with intraocular inflammation of unknown origin. each patient had either unilateral or bilateral moderate loss of visual acuity ranging from 20/25 to counting fingers. bilateral intraocular inflammation manifested by anterior and posterior segment cells, retinal lesions, macular exudate, and ...	1994	8053459
epidemiologic observations on infection with rochalimaea species among cats living in baltimore, md.	cats from several sources in baltimore, md, were tested for seropositivity to rochalimaea henselae and r quintana. co-infection with toxoplasma gondii or feline immunodeficiency virus was assessed as a risk factor for infection with rochalimaea spp. of 592 cats tested, 87 (14.7%) were seropositive for one or both rochalimaea spp, although titers to r henselae were significantly higher than those to r quintana. prevalence of seropositivity increased significantly with cat age and weight and was a ...	1994	8063598
bacillary angiomatosis: a new entity in acquired immunodeficiency syndrome.	since the recognition of the acquired immunodeficiency syndrome (aids) in 1981, previously rare infections and neoplasms have become increasingly common. bacillary angiomatosis, undescribed in the medical literature prior to 1983, is now second in frequency only to kaposi's sarcoma with respect to the cutaneous manifestations associated with human immunodeficiency virus (hiv) infection. caused by rochalimaea henselae, bacillary angiomatosis is easily treated, when diagnosed early, with erythromy ...	1994	8064965
detection of rochalimaea henselae dna by polymerase chain reaction from suppurative nodes of children with cat-scratch disease.	a polymerase chain reaction (pcr) assay was designed to amplify dna from rochalimaea henselae, rochalimaea quintana and afipia felis in the purulent material from lymph nodes in three patients with clinical cat-scratch disease (csd) and two patients with lymphadenitis from other causes. all of the patients with csd had positive immunofluorescent antibody serology for r. henselae, while none of the controls was positive. pcr amplification confirmed the presence of r. henselae dna and the absence ...	1994	7531320
cat scratch disease: past and present.		1994	7532423
recurrent pyogenic granuloma with satellitosis--a localized variant of bacillary angiomatosis?	a 27-year-old male patient had recurrent pyogenic granuloma with satellitosis. histologically, warthin-starry staining of the lesions revealed clumps of dark bacilli as found in patients with bacillary angiomatosis. igg antibodies against bartonella (rochalimaea) henselae were elevated as shown by an indirect immunofluorescence assay. the patient did not present an obvious risk for hiv infection or immunosuppression, and no antibodies against hiv-1 and hiv-2 were found. recurrent pyogenic granul ...	1994	7533011
persistent generalized lymphadenopathy and non-hodgkin's lymphoma in aids: association with rochalimaea henselae infection.	cat scratch disease, which is caused by infection with rochalimaea henselae, is often manifested as lymphadenopathy. r. henselae has also been isolated from human immunodeficiency virus (hiv)-positive patients with bacillary angiomatosis. in order to determine the frequency of r. henselae-reactive antibodies in hiv-positive patients with persistent generalized lymphadenopathy (pgl) or non-hodgkin's lymphoma (nhl), we tested a total of 124 hiv-positive patients for r. henselae-reactive immunoglob ...	1994	7496912
rochalimaea henselae infection in acquired immunodeficiency syndrome causing inflammatory disease without angiomatosis or peliosis. demonstration by immunocytochemistry and corroboration by dna amplification.	rochalimaea henselae causes bacillary angiomatosis, bacillary peliosis, and persistent bacteremia. difficult to cultivate, it is detectable in infected tissues by immunocytochemistry. this technique demonstrated r henselae in autopsy specimens obtained from three deceased patients who had acquired immunodeficiency syndrome, with pathologic tissue changes lacking neoangiogenic features. from the first patient, the cause of nodular collections of lymphocytes and nonepithelioid histiocytes in the l ...	1994	7506902
isolation and characterization by immunofluorescence, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, western blot, restriction fragment length polymorphism-pcr, 16s rrna gene sequencing, and pulsed-field gel electrophoresis of rochalimaea quintana from a patient with bacillary angiomatosis.	rochalimaea quintana was isolated from the blood of a french human immunodeficiency virus-infected patient with bacillary angiomatosis. the isolate showed the typical growth characteristics of rochalimaea species and was inert when typical biochemical testing was used. the purpose of the present work was to characterize and compare this new isolate with reference strains of r. quintana, rochalimaea vinsonii, and rochalimaea henselae by using immunofluorescence, sodium dodecyl sulfate-polyacrylam ...	1994	7519628
rochalimaea species stimulate human endothelial cell proliferation and migration in vitro.	rochalimaea henselae and r. quintana are associated clinically with proliferative neovascular lesions. the effect of rochalimaea species on human umbilical vein endothelial cell (huvec) proliferation and migration was evaluated in vitro. cocultivation of rochalimaea organisms with huvecs resulted in enhanced huvec proliferation. fibroblast proliferation was unaffected by r. henselae. huvecs were also stimulated to migrate by rochalimaea. when r. henselae organisms were disrupted and subjected to ...	1994	7523553
[rochalimaea henselae, afipia felis and cat-scratch disease].	several years ago, rochalimaea henselae has emerged as an agent of bacillary angiomatosis, bacillary peliosis and recurrent septicaemia that generally occur in patients infected with human immunodeficiency virus. an aetiologic role in cat scratch disease is also suspected widely on the basis of a serologic survey. its slow growth and its culture requirement explain that this pathogen, a gram-negative bacterium, could not be isolated until 1990. moreover, blood and tissue samples request lysis an ...	1994	7524253
cat-scratch disease in italy: a serological approach.	the results of studies on the serologic responses to afipia felis and rochalimaea henselae in suspected patients for cat scratch disease (csd) are illustrated. this preliminary study performed using indirect immunofluorescence assay, proved negative for a. felis and r. henselae in some patients and positive in others; in a few instances the test was positive for both organisms. additional microbiological and serological studies are needed to clarify the exact role of these microorganisms in caus ...	1994	7526131
fever, back pain and pleural effusion in a four-year-old boy.		1994	7526327
bacteriophage-like particle of rochalimaea henselae.	an extracellular particle approximately 40 nm in diameter was detected in culture supernatant from the fastidious bacterium rochalimaea henselae. this particle has at least three associated proteins and contains 14 kbp linear dna segments that are heterogeneous in sequence. the 14 kbp dna was also present in r. henselae cells as an extrachromosomal element for all 14 strains tested. despite attempts to induce lysis of r. henselae, plaque formation was not observed. a similar particle, also conta ...	1994	7527118
encephalitis associated with cat scratch disease--broward and palm beach counties, florida, 1994.	on august 14, 1994, the broward county public health unit of the florida department of health and rehabilitative services was notified of three children from pompano beach who were hospitalized with encephalitis attributed to cat scratch disease (csd). all three children (aged 5, 6, and 11 years) were previously healthy and had no histories of seizure disorders or diagnoses of csd. this report summarizes the investigation of these cases.	1994	7527121
cat scratch disease.		1994	7527210
positive predictive value of rochalimaea henselae antibodies in the diagnosis of cat-scratch disease.		1994	7527255
downregulation of human polymorphonuclear cell activities exerted by microorganisms belonging to the alpha-2 subgroup of proteobacteria (afipia felis and rochalimaea henselae).	intracellular pathogens have evolved effective mechanisms in order to survive in an intracellular environment, thus avoiding destruction by phagocytic cells. in this regard, a correlation between resistance to phagocytic killing and expression of pathogenic potency has been established. in this report, we have studied the interaction between human polymorphonuclear cells (pmn) and two gram-negative microorganisms, afipia felis and rochalimaea henselae, which belong to the alpha-2 subgroup of the ...	1994	7528238
cat scratch disease.		1994	7528453
persistent infection with bartonella (rochalimaea) henselae or afipia felis is unlikely to be a cause of chronic fatigue syndrome.		1994	7528553
neuroretinitis in cat-scratch disease associated with the macular star.		1994	7528909
[vascular tumors of the skin and soft tissue. overview of newly characterized entities and variants].	this review summarizes vascular tumours of skin and soft tissues that have been characterised in recent years. although most of them are very rare, knowledge about their reproducible clinicopathological features is important to avoid diagnostic pitfalls. these lesions include: bacillary angiomatosis, a vasoproliferative, pseudoneoplastic infection of immunocompromised patients caused by rochalimaea henselae; tufted angioma, a variant of lobular capillary haemangioma characterised by a "cannon-ba ...	1994	7824435
rochalimaea henselae infection. a new zoonosis with the domestic cat as reservoir.	to determine the reservoir and vector(s) for rochalimaea henselae, a causative agent of bacillary angiomatosis (ba) and cat scratch disease, and to estimate the percentage of domestic cats with r henselae bacteremia in the greater san francisco bay region of northern california.	1994	8301768
morphologically variable bacilli of cat scratch disease are identified by immunocytochemical labeling with antibodies to rochalimaea henselae.	the identification of the causative organisms of cat scratch disease (csd) has been elusive. the demonstration of warthin-starry stain-positive pleomorphic bacilli in lymph nodes of patients with csd and recent serologic and epidemiologic data suggest an etiologic role of rochalimaea henselae in csd. the authors studied lymph node biopsy specimens of 46 patients with illnesses clinically consistent with csd and found pleomorphic bacilli in 15 (33%). the organisms were labeled by polyclonal rabbi ...	1994	8178768
immunoelectrophoretic characterization and cross-reactivity of rochalimaea henselae, rochalimaea quintana and afipia felis.	the soluble antigens of rochalimaea henselae, rochalimaea quintana and afipia felis were characterized by crossed immunoelectrophoresis using bacterial sonicates as antigens against pooled hyperimmune rabbit sera. a precipitin pattern was drawn for each bacterium and shown to be reproducible and stable even when normal or preimmune rabbit serum was incorporated in the intermediate gel. by this technique 56 antigens were identified from r. henselae, 49 from r. quintana, and 39 from a. felis. the ...	1994	7534092
antibody response in rabbits infected with rochalimaea henselae, rochalimaea quintana and afipia felis.	antibody responses in three pairs of rabbits inoculated with live rochalimaea henselae, rochalimaea quintana and afipia felis were studied by enzyme immunoassay with whole-cell and bacterial sonicates as antigen. no differences in measured antibody responses were found with the two types of antigen preparation. two rabbits did not respond with antibody production. in the remaining rabbits there was a low-titred antibody response that showed no significant cross-reaction with related bacteria. af ...	1994	7534093
bacillary angiomatosis. clinical and histologic features, diagnosis, and treatment.	bacillary angiomatosis is a relatively new infection affecting primarily patients with human immunodeficiency virus or others with impaired host defenses. it presents most commonly with multiple red skin lesions, but visceral involvement may also occur, including involvement of the liver and spleen. because of the dermatologic manifestations, bacillary angiomatosis may be mistaken for kaposi's sarcoma. the diagnosis is made by identification of the characteristic histologic findings or genetic a ...	1994	8122945
rochalimaea henselae infections: newly recognized zoonoses transmitted by domestic cats.		1994	8144389
rochalimaea henselae organisms possess an elevated capacity of binding to peripheral blood lymphocytes from patients with cat scratch disease.	cat scratch disease (csd) is a clinical condition whose aetiological agent, according to recent findings, is of bacterial origin. two gram-negative bacteria are invoked as causative agents of csd, namely afipia felis and rochalimaea henselae. in this paper, five patients with suspected csd were studied in terms of binding capacity of a. felis and r. henselae to their own peripheral blood lymphocytes (pbl). this parameter was correlated with serum antibody titres to both a. felis and r. henselae, ...	1994	8152395
atypical cat-scratch disease: diagnosis by a serologic test for rochalimaea species.	three patients with atypical manifestations of cat-scratch disease (csd) had the diagnosis supported by a newly available serologic assay for antibodies to rochalimaea henselae. previously, csd has been a diagnosis of exclusion because no confirmatory test was readily available. atypical or severe cases of csd have often required detailed (and expensive) clinical and laboratory investigation. the use of rochalimaea-specific serologic tests may help avoid extensive diagnostic testing or invasive ...	1994	8153776
disseminated cat-scratch disease: detection of rochalimaea henselae in affected tissue.	an immunocompetent 9-year-old boy with disseminated cat scratch disease involving spleen, cervical and abdominal lymph nodes, skull, and one clavicle is reported. antibodies to rochalimaea quintana and r. henselae were detected, at increasing, then decreasing concentration. dna extracted from the biopsied skull lesion was amplified by polymerase chain reaction and hybridized with species-specific oligonucleotides proving the presence of r. henselae in affected tissue. our findings suggest that r ...	1994	8313920
[bacillary angiomatosis. a pseudoneoplastic infection in aids patients].	bacillary angiomatosis is a newly recognized pseudoneoplastic vascular disease seen in the setting of human immunodeficiency virus (hiv) infection. the disease is characterized by a cutaneous infection with reddish papules or nodules that are similar to pyogenic granulomas or kaposi's sarcoma in clinical appearance. it is caused by the mildly gram-negative bacillus rochalimaea henselae, which can be identified in tissue sections by means of warthin-starry stain. the historical, clinical, histopa ...	1993	8335458
cat scratch disease and rochalimaea henselae.		1993	8336627
development and evaluation of a blood-free medium for determining growth curves and optimizing growth of rochalimaea henselae.	two strains of rochalimaea henselae were used to optimize a blood-free growth medium. seven agar bases, four broths, and combinations of eight supplements were evaluated. acceptable growth was achieved in media containing fildes solution and hemin, with the best growth demonstrated in brucella broth or on brucella agar with 6 to 8% fildes solution and 250 micrograms of hemin per ml. r. henselae utilized hemin in concentrations six times that utilized by rochalimaea quintana. erythrocyte membrane ...	1993	8349767
detection of rochalimaea henselae in cat-scratch disease skin test antigens.	one of the diagnostic criteria for cat-scratch disease is a positive delayed-type hypersensitivity skin test reaction to an antigen prepared from purulent material aspirated from a cat-scratch disease-involved lymph node. polymerase chain reaction coupled with dna sequence analysis was used to identify organisms found in these skin test antigen preparations that may be responsible for eliciting this response. two independent sources of cat-scratch disease skin test antigens yielded dna sequences ...	1993	8376816
endocarditis caused by rochalimaea henselae.	a case of community-acquired, culture-negative, infective endocarditis was diagnosed in a 57-year-old construction worker. small, pleomorphic gram-negative rods were seen in brown-hopps tissue gram stains and warthin-starry silver stains. the organism was identified as rochalimaea henselae by polymerase chain reaction amplification and sequencing of the 16s rdna gene sequence. this is the first report of infective endocarditis caused by r henselae.	1993	8406424
bacteremia due to rochalimaea henselae in a child: practical identification of isolates in the clinical laboratory.	two closely related species of rochalimaea, rochalimaea quintana and rochalimaea henselae, are nutritionally fastidious but can be cultivated on bacteriologic media from the blood of patients with diverse clinical presentations. we report a case of culture-proven r. henselae bacteremia in a child with persistent fever. serologic evidence of infection by r. henselae was ascertained by testing sera at two intervals for immunoglobulin g or immunoglobulin m antibodies by enzyme immunoassay and immun ...	1993	8408560
syndrome of rochalimaea henselae adenitis suggesting cat scratch disease.	to describe a clinical syndrome of cat scratch disease caused by rochalimaea henselae, including methods for isolation of the organism from tissue and for identification.	1993	8430978
endocarditis caused by rochalimaea quintana in a patient infected with human immunodeficiency virus.	rochalimaea quintana and rochalimaea henselae are closely related, fastidious, gram-negative rickettsiae. thus far, the spectrum of human rochalimaea sp. infections has not included endocarditis. we describe a 50-year-old human immunodeficiency virus-positive man who developed endocarditis caused by r. quintana. dna relatedness studies, which compared our patient's blood culture isolate with known rochalimaea species, identified the organism as r. quintana. our report expands the spectrum of roc ...	1993	8458964
eia for detection of rochalimaea henselae-reactive igg, igm, and iga antibodies in patients with suspected cat-scratch disease.		1993	8501350
cat scratch disease in connecticut. epidemiology, risk factors, and evaluation of a new diagnostic test.	although cat scratch disease is commonly diagnosed in patients who have unexplained regional lymphadenopathy after encounters with cats, its epidemiology and the risk factors for disease are not clearly defined, and there is no generally accepted diagnostic test.	1993	8505963
[rochalimaea henselae infection. febrile pancytopenia and hepatic peliosis in a patient with hiv infection].	a case of rochalimaea henselae infection in an aids patient is reported. the r. henselae infection was revealed by febrile pancytopenia associated with liver and spleen enlargement. the diagnosis was made on the finding at histology of hepatic peliosis lesions. within these lesions warthin starry staining displayed bacilli that were identified as r. henselae. this case shows that all hiv-infected patients with these symptoms might be infected with this organism.	1993	8511079
bacillary angiomatosis of the spleen.	bacillary angiomatosis is a recently described vasoproliferative lesion associated with infection by a newly characterized rickettsial organism, rochalimaea henselae. most previous reports have described skin lesions in immunocompromised patients infected with human immunodeficiency virus. this is the first case report detailing the features of bacillary angiomatosis of the spleen occurring in a patient undergoing cytotoxic chemotherapy for disseminated ovarian carcinoma.	1993	8165007
proposals to unify the genera bartonella and rochalimaea, with descriptions of bartonella quintana comb. nov., bartonella vinsonii comb. nov., bartonella henselae comb. nov., and bartonella elizabethae comb. nov., and to remove the family bartonellaceae from the order rickettsiales.	dna hybridization data (hydroxyapatite method, 50 to 70 degrees c) indicate that rickettsia prowazekii, the type species of the type genus of the family rickettsiaceae, is substantially less closely related to rochalimaea species than was previously thought. the levels of relatedness of rickettsia prowazekii to rochalimaea species and to bartonella bacilliformis under optimal conditions for dna reassociation were 0 to 14%, with 25.5% or greater divergence in related sequences. when stringent rea ...	1993	8240958
ehrlichia chaffeensis and rochalimaea antibodies in kawasaki disease.	sera from 38 patients with kawasaki disease were tested for immunofluorescent antibodies to ehrlichia chaffeensis, rochalimaea henselae, and r. quintana oklahoma. only 2.5% of the patients tested positive for e. chaffeensis, and 5% were positive for r. henselae and r. quintana oklahoma. our data suggest that ehrlichia and rochalimaea spp. do not play a unique role in the etiology of kawasaki disease.	1993	8263201
cat-scratch disease mimicking rhabdomyosarcoma.	a 12-year-old boy who had injured his elbow presented with a lump in the epitrochlear area. originally thought to be a hematoma, then later myositis ossificans, this mass was actually a lymph node with large, reactive germinal centers. although stains for acid-fast bacilli, fungal organisms, bacteria, and cat-scratch disease were negative, serological testing for detection of rochalimaea henselae organisms found evidence of cat-scratch disease. we would like to remind orthopaedic surgeons to con ...	1993	8265222
[bacillary angiomatosis of the oral cavity in aids. a differential diagnosis of mucosal kaposi's sarcoma].	the diagnosis of bacillary angiomatosis (ba) was performed on a biopsy of a reddish nodular lesion of the gingival mucosa, in a patient seropositive for the h.i.v. it was based on the histopathological features, and by the demonstration of bacilli both on the warthin-starry stain and the electron microscopic study. a treatment by erythromycin was quickly effective. ba is an opportunistic infection seen essentially in patients with a.i.d.s., most often causing cutaneous involvement. the causative ...	1993	8278795
antimicrobial susceptibility of rochalimaea quintana, rochalimaea vinsonii, and the newly recognized rochalimaea henselae.	the susceptibility of an isolate of rochalimaea quintana, rochalimaea vinsonii and the newly recognized rochalimaea henselae to antimicrobial agents was determined by dilution in mueller-hinton agar supplemented with 5% sheep blood. two inocula of 10(2) and 10(4) cfu were used and tests were read after incubation at 5 and 7 days at 37 degrees c in an atmosphere enriched to 5% carbon dioxide. each isolate was susceptible to amoxycillin, third-generation cephalosporins, tetracyclines, macrolides a ...	1993	8288500
[bacillary peliosis in aids. anatomo-clinical study of 2 cases].	the authors report two cases of peliosis hepatis, occurring in patients with aids, who presented a persistent fever and an hepatomegaly. the liver biopsies showed areas of peliosis, where bacilli were observed by warthin-starry stain. in one case, techniques of molecular biology allowed the identification of rochalimaea henselae, pathogen involved in bacillary angiomatosis. this rickettsia has been newly recognized in the united-states, where 17 cases of bacillary peliosis have been published in ...	1993	8311862
polymerase chain reaction-based restriction fragment length polymorphism analysis of a fragment of the ribosomal operon from rochalimaea species for subtyping.	restriction endonuclease analysis of a polymerase chain reaction-amplified dna fragment which included the spacer region between the genes coding for 16s and 23s rrnas and a portion of the gene coding for 23s rrna (spacer + 23s) was done on 10 previously characterized clinical isolates of rochalimaea henselae, one clinical isolate of rochalimaea quintana, and the type strains of r. henselae, r. quintana, rochalimaea vinsonii, and bartonella bacilliformis. brucella abortus dna was not amplified b ...	1993	8102375
nucleotide sequence and comparison of the 5s ribosomal rna genes of rochalimaea henselae, r.quintana and brucella abortus.		1993	7685083
cat scratch disease and bacillary angiomatosis: aetiological agents and the link with aids.	the recent characterisation of a new species of the genus rochalimaea is the culmination of a ten year search for the identity of the agent of bacillary angiomatosis, a condition that is increasingly recognised in association with aids. this work has also implicated rochalimaea henselae in the aetiology of cat scratch disease contact with cats is a risk factor for both conditions. this report describes both diseases and reviews progress toward understanding their microbiology. r. quintana is als ...	1993	7693166
proposed tests for the routine identification of rochalimaea species.	a study was conducted to establish tests for the routine identification of rochalimaea species. strains used were reference strains of rochalimaea vinsonii and rochalimaea quintana, and a type strain and six human isolates of rochalimaea henselae. rochalimaea species were confirmed to be gram-negative, oxidase-negative, non-motile, urease-negative, indole-negative, catalase-negative, glucose-nonfermenting organisms which failed to grow on macconkey agar. further testing of the organisms in a com ...	1993	7694852
serological response to "rochalimaea henselae" antigen in suspected cat-scratch disease.	there are no generally accepted diagnostic tests for cat-scratch disease (csd), the cause of which is unknown. during the development of an indirect fluorescence antibody (ifa) test for detection of antibodies to "rochalimaea henselae", sera from patients with csd were found to have high titres to r henselae antigens. further tests with this assay showed that 36 (88%) of 41 patients with suspected csd had serum titres of 64 or more to r henselae antigen, that there was a low prevalence (3%) of s ...	1992	1351130
naturally occurring "rochalimaea henselae" infection in domestic cat.		1992	1354314
possible role of rochalimaea henselae in pathogenesis of aids encephalopathy.		1992	1357365
characterization of a novel rochalimaea species, r. henselae sp. nov., isolated from blood of a febrile, human immunodeficiency virus-positive patient.	isolation of a rochalimaea-like organism from a febrile patient infected with human immunodeficiency virus was confirmed. analysis of 16s rrna gene sequences, together with polymerase chain reaction and restriction endonuclease length polymorphism analysis of a portion of the citrate synthase gene, demonstrated that the agent is closely related to members of the genus rochalimaea and that the isolate is genotypically identical to the presumptive etiologic agent of bacillary angiomatosis. however ...	1992	1371515
identification of the rochalimaea henselae 16s rrna sequence in the liver of a french patient with bacillary peliosis hepatis.		1992	1385550
isolation of rochalimaea species from cutaneous and osseous lesions of bacillary angiomatosis.	bacillary angiomatosis is characterized by vascular lesions, which occur usually in patients infected with the human immunodeficiency virus (hiv). a newly described gram-negative organism, rochalimaea henselae, has been associated with cutaneous bacillary angiomatosis, but no organism has been isolated and cultivated directly from cutaneous tissue.	1992	1435899
immunocytochemical identification of rochalimaea henselae in bacillary (epithelioid) angiomatosis, parenchymal bacillary peliosis, and persistent fever with bacteremia.	we report the immunocytochemical identification of rochalimaea henselae, a newly recognized fastidious, gram-negative, warthin-starry-positive organism, as the common pathogen in bacillary angiomatosis (ba), bacillary peliosis (bp) of the liver and spleen, and persistent fever with bacteremia in immunocompromised patients. immunogenic proteins of the r. henselae strain isolated from the blood of a febrile immunocompromised patient with bp of the liver were used to produce primary immune serum in ...	1992	1530106
rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis.	nine strains of rochalimaea spp. that were isolated from patients over a period of 4.5 years were characterized for their enzyme activities, cellular fatty acid compositions, and dna interrelatedness among rochalimaea spp., bartonella bacilliformis, and afipia felis (cat scratch disease bacillus). all except one isolate, which was rochalimaea quintana, were determined to belong to a newly proposed species, rochalimaea henselae sp. nov. after recovery from clinical material, colonies required 5 t ...	1992	1537892
rochalimaea henselae causes bacillary angiomatosis and peliosis hepatis.	recent studies have demonstrated that a newly described agent of persistent bacteremia, rochalimaea henselae, and the agent of bacillary angiomatosis are both closely related to rochalimaea quintana. bacillary peliosis hepatis seemed likely to have the same etiologic agent as bacillary angiomatosis. we sought these pathologic changes in patients from whom r henselae was cultivated.	1992	1546924
relapsing illness due to rochalimaea henselae in immunocompetent hosts: implication for therapy and new epidemiological associations.	two previously healthy, immunocompetent men had persistent rochalimaea henselae bacteremia with clinical relapses after courses of antibiotics to which the isolates were ultimately demonstrated susceptible in vitro. both had sustained tick bites prior to their illnesses, thus demonstrating an association not previously identified, although suspected. the first patient had relapsing fever, constitutional symptoms, and an episode of aseptic meningitis despite therapy with amoxicillin, then with do ...	1992	1562660
murine antibody responses distinguish rochalimaea henselae from rochalimaea quintana.	rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. detection of a specific antibody response to r. henselae infection may represent an alternative to cultivation as a means of diagnosis. we assessed the specificity of induced murine antibodies for antigens from r. henselae and the closely related species r. quintana. groups of cd-1 mice were inoculated with whole organisms of six strains of r. henselae and two of r. quintana. pre- and p ...	1992	1629326
multiplex sybr® green-real time pcr (qpcr) assay for the detection and differentiation of bartonella henselae and bartonella clarridgeiae in cats.	a novel sybr® green-real time polymerase chain reaction (qpcr) was developed to detect two bartonella species, b. henselae and b. clarridgeiae, directly from blood samples. the test was used in blood samples obtained from cats living in animal shelters in southern brazil. results were compared with those obtained by conventional pcr targeting bartonella spp. among the 47 samples analyzed, eight were positive using the conventional pcr and 12 were positive using qpcr. importantly, the new qpcr de ...	0	24626408
bartonella spp. bacteremia and rheumatic symptoms in patients from lyme disease-endemic region.	bartonella spp. infection has been reported in association with an expanding spectrum of symptoms and lesions. among 296 patients examined by a rheumatologist, prevalence of antibodies against bartonella henselae, b. koehlerae, or b. vinsonii subsp. berkhoffii (185 [62%]) and bartonella spp. bacteremia (122 [41.1%]) was high. conditions diagnosed before referral included lyme disease (46.6%), arthralgia/arthritis (20.6%), chronic fatigue (19.6%), and fibromyalgia (6.1%). b. henselae bacteremia w ...	0	22516098
prevalence of anaplasma, bartonella and borrelia species in haemaphysalis longicornis collected from goats in north korea.	north korea is located on the northern part of the korean peninsula in east asia. while tick-borne pathogens of medical and veterinary importance have been reported from china and south korea, they have not been reported from north korea. to screen for zoonotic tick-borne pathogens in north korea, ticks were collected from domestic goats. a total of 292 (27 nymph, 26 male, 239 female) haemaphysalis (h.) longicornis were collected and assayed individually for selected tick-borne pathogens. a tota ...	0	26645342