Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| experimental ovine salmonellosis (salmonella abortusovis): pathogenesis and vaccination. | salmonella enterica subsp. enterica ser. abortusovis, a sheep-adapted serotype, causes a contagious disease. abortion is the major symptom and the main source of contamination. research on this ovine disease may aid farmers, but may also contribute to comparative biological knowledge. innate resistance partly controlled by the ity locus, increased resistance to reinfection and humoral and t-cell-mediated immunity were observations gained with a murine model. in ewes, abortion regularly occurs fo ... | 1990 | 2101486 |
| thyroiditis due to brucella melitensis--report of two cases. | two cases of thyroiditis due to brucella melitensis are reported. both occurred in foreigners working in switzerland and presented with characteristic symptoms of thyroiditis which masked those of brucellosis. cultures were diagnostic. one patient had concomitant thyroid malignancy. both showed normal thyroid function and were cured following antibiotic treatment. | 1990 | 2114370 |
| brucella suis s2, brucella melitensis rev. 1 and brucella abortus s19 living vaccines: residual virulence and immunity induced against three brucella species challenge strains in mice. | live attenuated brucella suis s2 vaccine was compared to living vaccines b. abortus s19 and b. melitensis rev. 1 in mice. residual virulence was estimated by ability to multiply and persist in spleen and lymph nodes. immunogenicity was estimated by spleen counts of control and vaccinated mice challenged either with the reference b. abortus 544 strain or with virulent b. melitensis h38 and b. suis 1330 strains. s2 vaccine had lower residual virulence; expressed as 50% recovery time, persistence w ... | 1990 | 2123586 |
| [simultaneous isolation of brucella melitensis and mycobacterium tuberculosis in pleural empyema]. | 1990 | 2129196 | |
| isolation, identification, and characterization in israel of brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indicating the evolution of a new variant. | during characterization by conventional biotyping tests of numerous brucella melitensis isolates obtained in israel in the last 2 years, we identified several strains of the biovar 1 serotype that are atypically susceptible to dyes and penicillin. their coisolation from two brothers in a family that rears sheep and from the milk of one of their sheep and the prevalence of such strains in two distinct geographical zones in israel provide epidemiological support for the notion that a new variant h ... | 1990 | 2191005 |
| the interaction of brucella melitensis 16-m and caprine polymorphonuclear leukocytes. | in the present work, a study about the phagocytosis and intracellular killing of the polymorphonuclear leukocytes (pnmls) of goat in animals clinically healthy, vaccinated and inoculated experimentally with brucella melitensis has been done. during 6 weeks postvaccination and postinfection, the evolution in these animals has been studied. although animals were inoculated or vaccinated, there was influence in the phagocytosis and intracellular killing phases, even though a very low indices in thi ... | 1990 | 2208970 |
| the isolation and serology of brucella melitensis in a flock of goats in central rsa. | brucella melitensis biotype 1 was isolated in pure culture from the lungs, liver, spleen, kidney, stomach contents, abomasum and brain of an aborted caprine (boer goat) foetus in the district of cullinan near pretoria. the 18 does and 1 ram in the flock of boer goates were examined serologically by means of the complement fixation (cf) test, using brucella abortus antigen. six weeks later they were examined again, using b. abortus as well as b. melitensis biotype 1 antigens. no significant diffe ... | 1990 | 2216349 |
| analysis of immune response: comparison of immunoblots after isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis using cytoplasmic protein extract from brucella. | analysis of the immune response towards the facultative intracellular bacterium, brucella melitensis, was studied by immunoblotting after either isoelectric focusing (ief) or sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page). a cytoplasmic extract (cpe) of brucella melitensis was used as antigen to analyse the response in 17 sera from naturally infected goats. cpe analysed by ief exhibited 25 proteins within the ph range of 4.35 to 6. immunoblotting revealed most of the staine ... | 1990 | 2227361 |
| the effect of the ph of various dairy products on the survival and growth of brucella melitensis. | in this study we report the effect of the ph of various dairy products on the survival and growth of brucella melitensis. the growth patterns of b. melitensis in broth media at different phs (ranging between 3 and 9) were studied for up to four weeks, to standardize the growth of the organism at each ph. these growth patterns were compared with those of the same organism growing in different dairy products [milk, soft cheese, yoghurt, and buttermilk (shaneena)] under the same growth conditions. ... | 1990 | 2256775 |
| diagnosis and treatment of 106 cases of human brucellosis. | during the year 1987, 106 cases of human brucellosis were studied prospectively at the jordan university hospital. the disease was more often diagnosed among adults (73.6%) than children (26.4%). serious clinical complications were observed in 5.7% patients. an initial brucella antibody titre greater than or equal to 160 proved to be reliable in confirming suspected cases of acute and subacute brucellosis. culture of blood was found to be more sensitive (44.4%) and significant (p less than 0.02) ... | 1990 | 2299182 |
| isolation of brucella melitensis biovar 3 from a chamois (rupicapra rupicapra) in the southern french alps. | systemic brucellosis caused by brucella melitensis biovar 3 was identified in a chamois (rupicapra rupicapra) near the parc national des ecrins in the southern french alps (france). clinical signs included orchiepididymitis, polyarthritis, blindness and various neurological signs; necropsy findings included numerous calcified foci in testis, epididymis, kidney, subcutaneous connective tissue and brain. brucella sp. were identified in brain by indirect immunofluorescence and b. melitensis biovar ... | 1990 | 2304191 |
| childhood brucellosis: a study of 102 cases. | one hundred two children, 45 days to 14 years of age, with proven brucellosis were studied to illustrate the epidemiologic, clinical and laboratory findings and to assess the outcome of antimicrobial therapy. the main source of infection was the consumption of raw milk in 80% of the patients. the predominant presenting symptoms and signs were fever, arthralgia, malaise, weight loss, arthritis, hepatosplenomegaly and lymphadenopathy. brucella melitensis was isolated from 75% of 87 patients. diver ... | 1990 | 2314956 |
| [childhood brucellosis in the negev]. | 33 children (22 girls) with brucellosis seen between 1972-1988 were studied retrospectively. all but 1 were bedouins. the mean age at diagnosis was 9.8 years (range: 17 months-17 years). duration of illness prior to diagnosis was less than 1 week in 13 (39%), 1-4 weeks in 8 (24%) and 1-3 months in 10 (30%). in 2 cases the symptoms lasted 6 and 8 months, respectively, before diagnosis. presenting symptoms included fever (85%), articular involvement (65%), hepatomegaly (45%) and splenomegaly (33%) ... | 1990 | 2283118 |
| successful treatment of brucella melitensis endocarditis with pefloxacin. | 1990 | 2387299 | |
| brucella melitensis endogenous endophthalmitis. | a 17-year-old female patient was examined and found to have brucella-sp.-induced endophthalmitis in her left eye. the specific organism was brucella melitensis, biotype 3, a known virulent organism. serum agglutination titres from two independent laboratories were positive, yet of different levels: 1:320 and 1:1,280. although brucellosis is thought to be eradicated in many countries, it prevails in agrarian environments, where it can be transmitted to man. this report is the first evidence to co ... | 1990 | 2392274 |
| isolation of brucella melitensis from human sperm. | 1990 | 2351148 | |
| chronic neurobrucellosis due to brucella melitensis. | a 20-year-old male turkish immigrant to norway suffering from severe chronic neurobrucellosis with spastic paraplegia and deafness is presented. the diagnosis was established by isolation of brucella melitensis from cerebrospinal fluid (csf) culture. brucella antibody agglutination titers were high in serum and csf. in spite of intensive, prolonged treatment with a combination of trimethoprim-sulfamethoxazole (tpm-smz), rifampicin and doxycycline, the course of the illness was characterized by r ... | 1990 | 2356445 |
| serology, clinical manifestations and treatment of brucellosis in different age groups. | we prospectively studied 339 patients diagnosed of brucellosis over a six year period in order to evaluate the clinical and serological characteristics of brucellosis in the elderly. 319 patients (94.1%) were under 65 years of age (group a), and 20 patients (5.9%) were older than 65 (group b). no patient in group b developed splenomegaly as opposed to 69 (21.6%) in group a (p less than 0.05). the percentage of positive blood cultures (brucella melitensis isolated in all cases) was 72.1% in group ... | 1990 | 2365466 |
| antibacterial activity of lomefloxacin against brucella melitensis. | the in vitro activity of lomefloxacin was tested against 114 clinical isolates of brucella melitensis. comparison was made with ciprofloxacin, tetracycline, gentamicin, rifampin, and trimethoprim-sulfamethoxazole. lomefloxacin inhibited 113 (99.1%) of the 114 strains tested at less than or equal to 0.5 microgram/ml. it was comparable to ciprofloxacin, tetracycline and gentamicin in antimicrobial potency. one strain that was previously susceptible to ciprofloxacin and had become resistant after t ... | 1990 | 2383977 |
| [the antigenic relationship between brucella abortus, brucella melitensis and yersinia enterocolitica serotype 0:3 and 0:9]. | antigenic interrelationship between b. abortus, b. melitensis, y. enterocolitica serotype 0:3 and serotype 0:9 were investigated in hyperimmün sera obtained from rabbits, by using agglutination and agglutinin absorbtion tests. agglutination was determined at 1/2560 titer of b. abortus and b. melitensis hyperimmün sera when their own antigens were used. on the other hand, these sera agglutinated y. enterocolitica serotype 0:9 antigen at 1/1280 and 1/320 titer respectively. as it was seen that hig ... | 1990 | 1704479 |
| brucellosis in patients with heart disease: when should endocarditis be diagnosed? | three patients, 2 with congenital valvular heart disease and 1 with a prosthetic aortic valve developed brucellosis. brucella melitensis was isolated from blood of all 3 patients. the clinical and microbiological features suggested brucella endocarditis and following successful antibiotic therapy, no surgery was required. the salient diagnostic features are discussed with emphasis on the management and prognosis of patients with brucella endocarditis. | 1990 | 2073648 |
| multicenter prospective study of treatment of brucella melitensis brucellosis with doxycycline for 6 weeks plus streptomycin for 2 weeks. | the effectiveness of treating human brucellosis caused by brucella melitensis with a 6-week course of doxcycline plus streptomycin for 2 of those weeks was analyzed by a multicenter prospective study of 139 patients. subjects with central nervous system involvement, endocarditis, or spondylitis were excluded from the study. all but 5 of the 139 patients completed the full treatment schedule and became afebrile in the first week of therapy. four patients suffered relapses during the follow-up per ... | 1990 | 2193624 |
| the isolation of brucella melitensis biovar 3 from a testicular hygroma of a ram in a nomadic flock of sheep and goats in western sudan. | brucella melitensis biovar 3 was isolated from a testicular hygroma in a ram from a nomadic flock of sheep and goats serologically positive for brucellosis and with a history of occasional abortions. the affected testis was enlarged, extensively damaged and contained fluid. the isolation of this biovar identifies the need for further investigations into the prevalence of brucellosis and the particular biovars involved in nomadic animals. | 1990 | 2079561 |
| development of ciprofloxacin resistance in brucella melitensis. | 1990 | 2329110 | |
| brucella meningitis. | a 36-year-old hispanic man came into the emergency department with nonspecific symptoms (headache, myalgias, low-grade temperature, and low white blood cell count) and was diagnosed with brucella meningitis. the patient said he had consumed unpasteurized goat's milk and cheese in mexico, and had been treated 3 months previously for a febrile illness diagnosed as malta fever (brucellosis). cultures of both the blood and cerebrospinal fluid yielded brucella melitensis. blood agglutinin results for ... | 1990 | 2293833 |
| osteoarticular brucellosis in children. | the findings in 40 children (24 female, 16 male) with osteoarticular complications of brucellosis are presented. raw milk was the main source of infection. most patients had acute onset of fever, arthralgia, and myalgia. arthritis was the presenting symptom in all patients, of whom two also had osteomyelitis. arthritis was monoarticular in 28 (70%) of 40 cases with predilection for large weight-bearing joints. spine and small joints were spared. brucella melitensis was detected in 23/31 (74%) ca ... | 1990 | 2256735 |
| [a case of human brucellosis caused by accidental injection of animal vaccine]. | the authors describe the case of an acute brucellosis in a young veterinarian due to accidental injection of animal vaccine. the past history of the patient and the serological follow-up of the animals contacted by the professional did not evidence other risk contacts. the case evidence the real possibility that attenuated brucella melitensis strains used in animal's vaccination can represent a source of occupational risk for this infection. in addition, it is important to notify similar cases t ... | 1990 | 2136335 |
| ocular brucellosis. | though brucellosis has been eradicated from many countries, it remains a health problem in many developing countries. we report hereon a 34-year-old woman who presented with recurrent attacks of uveitis unresponsive to treatment with steroids. she was found to have systemic brucellosis, and brucella melitensis was isolated from a paravertebral abscess. the condition responded to systemic antibiotics, and she completely recovered from her uveitis. | 1990 | 2337555 |
| recurrent enterocolitis-like symptoms as the possible presenting manifestations of neonatal brucella melitensis infection. | a premature infant had three episodes of enterocolitis-like symptoms during the first three months of life. brucella melitensis was isolated in a prolonged blood culture on day 85. the clinical manifestations disappeared under treatment and did not recur. a maternofetal transmission is suggested in this case. the importance of prolonged blood cultures when brucellosis is epidemiologically suspected is emphasized. | 1990 | 2386068 |
| concomitant neurocysticercosis and brucellosis. | a young mexican woman had headache and left arm weakness develop shortly after immigrating to the united states. a solitary cerebral cysticercus was found at surgery, but, instead of the expected finding of clear fluid, the cyst contained pus from which brucella melitensis was cultured. although the patient had no signs or symptoms suggestive of brucellosis, agglutination studies revealed igm and igg antibodies consistent with active brucellosis. clinicians should be alert to the possibility of ... | 1990 | 2244597 |
| [cloning of brucella genes in escherichia coli k12 cells and analysis of products of the cloned genes]. | the libraries of brucella melitensis 565 and brucella abortus 99 in escherichia coli cells have been constructed. some clones of escherichia coli producing the specific brucella antigens have been found in immunological tests with brucella antiserum. two strains producing antigens have been characterized, one being from brucella melitensis 565 and another from brucella abortus 99 clone libraries . both strains synthesize two antigens that were studied by immunoelectrophoresis, immunoblotting aft ... | 1990 | 1701518 |
| selection of a brucella vaccine strain of low residual virulence by chemical mutagenesis. | following incubation of the oral vaccine brucella suis strain 2 with diethyl sulphate (des), a mutant designated strain s105 was selected by screening surviving bacteria for reduced virulence for mice. strain 105 also showed low residual virulence for guinea-pigs and, unlike the parent strain, did not initiate abortion in pregnant sheep and goats after parenteral administration. nevertheless, it was as effective as the parent strain in stimulating protective immunity to brucella melitensis when ... | 1989 | 2795638 |
| [sacroiliitis caused by brucella melitensis]. | technetium scintigraphy established sacroiliitis in a 21-year-old man who had complained of hip pain for two weeks. brucella melitensis was cultured from hip-joint fluid. he was treated with 160 mg trimethoprim and 800 mg sulphamethoxazole, three times daily for six weeks. he also had prophylactically 600 mg rifampicin daily by mouth for the last three weeks. he was symptom-free four weeks after onset of treatment. at follow-up one year later there was no sign of recurrence. brucella titres and ... | 1989 | 2917488 |
| [persistence of serologic and allergic reactions in ewes after vaccination with the h.38 or rev.1 vaccines in a brucellosis (brucella melitensis) enzootic area]. | in a flock of 2,000 ewes located in an endemic area of brucella melitensis infection, subjected to systematic vaccination of lambs either with killed and adjuvanted b. melitensis h.38 vaccine from 1973 to 1978, or with living b. melitensis rev.1 vaccine since 1979, reactions to allergic intrapalpebral test, complement fixation test and rose bengal plate test were recorded in small groups of animals vaccinated four, three, two or one years ago. four years after h.38 vaccination, 39 ewes out of 40 ... | 1989 | 2930135 |
| comparative analyses of proteins extracted by hot saline or released spontaneously into outer membrane blebs from field strains of brucella ovis and brucella melitensis. | sheep infected with brucella ovis produce antibody responses to the rough lipopolysaccharide and to proteins present in hot saline (hs) extracts of b. ovis (j. i. riezu-boj, i. moriyón, j. m. blasco, c. m. marín, and r. díaz, j. clin. microbiol. 23:938-942, 1986). the distribution and antigenic relatedness of proteins in hs extracts and in outer membrane blebs were established by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blotting for 41 strains of b. ovis and 26 strai ... | 1989 | 2651307 |
| brucellosis: an unusual cause of a child's fever of unknown origin. | a 3-year-old wisconsin native with the symptoms of chronic fever, hepatosplenomegaly, and inability to walk, was found to have an elevated anti-brucella titer. blood and bone marrow cultures grew brucella melitensis, biotype 3. this infection was likely acquired during a trip to mexico several months earlier during which the child had ingested raw milk. the patient's father was also discovered to have brucellosis. both were successfully treated with antibiotic therapy. although brucellosis has b ... | 1989 | 2728470 |
| monocyte locomotion in anergic chronic brucellosis patients: the in vivo effect of ascorbic acid. | in 14 patients suffering from relapsing chronic brucellosis who were anergic to brucella antigens, we have studied peripheral blood monocyte random migration and chemotaxis against non-specific and specific leukoattractants, as well as plasma and monocyte ascorbic acid levels. we found that all parameters studied, were significantly beneath normal, when compared to normal subjects. after the oral administration of ascorbic acid at a daily dose of 1gr for 15 consequetive days, random and directed ... | 1989 | 2760414 |
| definition of brucella a and m epitopes by monoclonal typing reagents and synthetic oligosaccharides. | the paradigm that brucella a and m epitopes are simultaneously expressed on single cells and within one antigen molecule was reinvestigated by using polysaccharide-specific murine monoclonal antibodies. monoclonal antibodies were generated to the m antigen of brucella melitensis 16m. chemically defined lipopolysaccharides and o polysaccharides from brucella abortus 1119-3, b. melitensis 16m, and yersinia enterocolitica o:9 were used to dissect the binding profiles of the b. melitensis antibodies ... | 1989 | 2474505 |
| brucellosis in the gezira area, central sudan. | twenty-nine patients presenting with symptoms and signs suggestive of brucellosis in the gezira area, central sudan, were studied. the majority of the patients (76%) were found to have a combined infection of both brucella abortus and brucella melitensis with titres of 1/160 and above. the social habit of eating raw meat, e.g. raw liver or other offal with spices (marrara or umfitfit) is found to be an important epidemiological factor in contracting the disease. raw milk consumption, since it is ... | 1989 | 2496237 |
| comparative in vitro activities of ofloxacin, difloxacin, ciprofloxacin, and other selected antimicrobial agents against brucella melitensis. | the in vitro activities of three quinolones (ofloxacin, difloxacin, and ciprofloxacin) were compared with those of trimethoprim-sulfamethoxazole, streptomycin, tetracycline, and rifampin against 47 brucella melitensis strains. ofloxacin was the most active of the test antimicrobial agents. it inhibited 90% of b. melitensis strains at a concentration of 0.02 micrograms/ml. | 1989 | 2802568 |
| acute calcular cholecystitis associated with brucella melitensis. | 1989 | 2919595 | |
| analysis of brucella lipopolysaccharide with specific and cross-reacting monoclonal antibodies. | monoclonal antibodies which bind brucella a lipopolysaccharide (lps)-specific, m lps-specific, or cross-reactive epitopes were used as reagents in quantitative dot blot, western blot (immunoblot), and immunoprecipitation analysis of brucella whole cells, whole-cell extracts, and purified lps preparations. this set of monoclonal antibodies detected four unique epitopes on brucella lps. the specificity of monoclonal antibodies reactive with brucella unique (a and m) and common (c and c/y) lps epit ... | 1989 | 2479655 |
| horizontal transmission in sheep and delayed clearance in guinea pigs and mice of a brucella melitensis rev. i mutant. | virulence assays in guinea-pigs, mice and sheep with a rev. i mutant strain (fsa) demonstrated that the latter had increased virulence in comparison with a reference strain. the mutant showed slower clearance in guinea-pigs and mice when compared with standard rev. i strains and horizontal transmission was achieved in 2 sheep. | 1989 | 2607538 |
| susceptibility of brucella melitensis to fluoroquinolones. | in vitro activity of four fluoroquinolones and four other antibacterial agents was tested against clinical isolates of brucella melitensis. initially all the 146 isolates studied were inhibited by 0.06-0.5 mg/l of ciprofloxacin and fleroxacin and 0.12-0.5 mg/l of pefloxacin or norfloxacin. one of these isolates developed resistance during therapy with ciprofloxacin, with a rise in mic from 0.06 mg/l to more than 5.0 mg/l. this strain also showed cross-resistance to all other quinolones. all the ... | 1989 | 2632216 |
| [bovine brucellosis caused by brucella melitensis in france]. | each brucella species is known to have a definite host preference. in this respect, brucellosis in cattle is primarily due to b. abortus. as part of an investigation of the species and biovars responsible for bovine brucellosis in france, 312 strains have been examined since 1978 by the methods recommended by the subcommittee on the taxonomy of the genus brucella. of these, 264 (85%) were indeed classified as b. abortus members. however the 48 others (15%) had all the characteristics that define ... | 1989 | 2930137 |
| experimental treatment of brucella melitensis infection in sheep with oxytetracycline alone or combined with streptomycin. | the efficacy of oxytetracycline (otc) alone or combined with streptomycin in the treatment of 118 najdi ewes believed to have been naturally infected with brucella melitensis, was evaluated by culture of selected tissues and organs at slaughter. groups of sheep were given 250, 500 or 1,000 mg of otc intraperitoneally (i/p) daily for six weeks and in the respective groups at necropsy 52, 69 and 100% of sheep were found to be brucella-free. treatment with 250 mg otc (daily for six weeks i/p) combi ... | 1989 | 2799947 |
| discrimination between sheep antibodies to brucella melitensis and to brucella ovis. | when preparations containing smooth brucella abortus lipopolysaccharide (lps) were used as antigen in an elisa, strong positive reactions were obtained with sera from sheep infected with brucella melitensis or with brucella ovis. oxidation of the lps with sodium metaperiodate greatly reduced the extent of the cross-reactions with antisera to b. ovis, with little effect on the reactions with antisera to smooth b. melitensis. periodate oxidation of hot saline extract (hsx) antigen of b. ovis marke ... | 1989 | 2800307 |
| treatment of brucella melitensis infection in mice by use of liposome-encapsulated gentamicin. | liposomes with entrapped gentamicin were used to treat mice with infection attributable to brucella melitensis. liposomes bearing positive charge and formed by egg yolk lecithin, cholesterol, and stearylamine were effective in the elimination of b melitensis residing in liver and spleen. negatively charged liposomes, formed by egg yolk lecithin, cholesterol, and dicetyl phosphate were also effective in suppression of the infection in liver, but were less so in suppression of the infection in the ... | 1989 | 2802318 |
| determination by enzyme-linked immunosorbent assay of immunoglobulin g (igg), igm, and iga to brucella melitensis major outer membrane proteins and whole-cell heat-killed antigens in sera of patients with brucellosis. | an enzyme-linked immunosorbent assay was used to compare brucella melitensis major outer membrane proteins (momp) and whole-cell heat-killed antigens (hk) in measuring antibrucella immunoglobulin g (igg), igm, and iga in sera of brucellosis patients and controls. antibodies to momp were generally similar to those against hk, and the correlation coefficients between the two antigens and igg, igm, and iga in patients varied between 0.73 and 0.94. both antigens are comparably suitable in detecting ... | 1989 | 2768476 |
| in vitro determination of phagocytosis and intracellular killing of brucella melitensis by goat's polymorphonuclear phagocytes. | one hundred and forty samples of goats serum taken from animals suspected of having brucellosis, have been studied by trying to assess the phagocytosis in vitro of brucella melitensis rev-1 by polymorphonuclear neutrophil phagocytes opsonized with antibrucella serum. the serum agglutination test and the coombs antiglobulin test were carried out with the serum samples. the opsonization ingestion, intracellular killing and germ killing power of the serum were also assessed. the results demonstrate ... | 1989 | 2776406 |
| the changing epidemiology of human brucellosis in texas, 1977-1986. | from 1977-1986, a total of 331 laboratory confirmed cases of human brucellosis were reported in texas. the annual number of cases ranged from 13 in 1977 to 84 in 1983. males accounted for 66% of the cases. cases ranged in age from one to 92 years, with 57% between the ages of 20 and 49 years. brucella melitensis infections accounted for 66% of the bacteriologically confirmed cases. infection with brucella canis was identified in four patients. annual incidence rates were higher in hispanics in e ... | 1989 | 2787107 |
| brucella melitensis cerebellar abscess. | 1989 | 2794569 | |
| characteristic cutaneous lesions in patients with brucellosis. | among 436 cases of brucellosis included in a 12-year prospective protocol, we identified 27 patients (6%) with cutaneous lesions (13 men and 14 women; mean age, 35.1 +/- 16.5 years). twenty-one patients had positive blood cultures for brucella melitensis. a disseminated violet-erythematous, papulonodular eruption (20 cases) and erythema nodosum-like lesions (three cases) were the most frequent eruptions observed, appearing during the initial episode of the disease or in relapse. histologic findi ... | 1989 | 2923445 |
| dot-enzyme immunoassay for visual detection of brucella melitensis antibodies. | an indirect enzyme-linked immunosorbent assay, using antigen coupled to paper, has been adapted for the detection of brucella melitensis antibodies. optimum conditions were achieved by incubation of 1 ml of diluted serum with a single piece of paper coated with purified brucella antigens for a period of one hour, and by addition of a goat anti-human enzyme conjugate antibody for one hour again. under these conditions 80 human sera were examined and the results obtained were compared with wright ... | 1989 | 2490400 |
| epidemiology and diagnosis of human brucellosis in jordan. | in recent reports, brucellosis has emerged as an important public health problem in the east mediterranean region and jordan is no exception. in 1986 and 1987, the minimal incidence rates per 100,000 persons in jordan were 33.2 and 46.2, respectively, with no sex difference. low incidence rates were confined to those below 4 and over 55 years of age. a seasonal incidence seen in 1986 was repeated in 1987 with maximal rates in the spring and summer months, exhibiting a strong connection with the ... | 1989 | 2738993 |
| brucellosis in a child complicated with multiple brain abscesses. | a 4-year-old boy developed symptoms consistent with brucellosis and was treated with combined streptomycin and tetracycline. he had a high brucella agglutinin titer. however, he continued to have headache and papilledema. a brain ct revealed 6 large abscesses. brucella melitensis was isolated from abscess material. the boy recovered completely after drainage therapy. | 1989 | 2756344 |
| apyrexic brucella melitensis aortic valve endocarditis. | the case of a young shepherd with brucella melitensis aortic valve endocarditis is presented. his illness ran an afebrile course and was also complicated by disseminated intravascular coagulation (dic), nephritis, hepatitis and peritonitis, all of which responded well to supportive measures and a combination of tetracycline, trimethoprim-sulphamethoxazole and amikacin sulphate. the fact that even the most severe case of brucellosis can present without fever is stressed. | 1989 | 2635455 |
| cellular responses to "brucelline inra" during the experimental brucellosis of the mouse. | "brucelline inra" is an aqueous extract of a rough strain of brucella melitensis that is used to test cutaneous delayed hypersensitivity in man and animals. the cellular response of mice (either normal, or sensitized by a previous brucella infection) was investigated with local implanted "cell-traps" and by immunological testing in vitro. brucelline markedly reduced the number of polymorphonuclear cells in the local infiltrate of sensitized animals during the early, nonspecific phase of the resp ... | 1989 | 2659500 |
| laboratory survey of fluoroquinolone activity. | fluoroquinolones are active against a wide variety of bacteria. the antibacterial spectra of fluoroquinolones encompass staphylococci, bacillus species, and corynebacterium species implicated in infections of the immunocompromised host; enterobacteriaceae; most intestinal pathogens; and many gram-negative organisms commonly causing nosocomial infections. haemophilus influenzae, haemophilus ducreyi, neisseria gonorrhoeae, neisseria meningitidis, and branhamella catarrhalis are highly susceptible ... | 1989 | 2672262 |
| brucella endocarditis. report of one case and review of the literature. | we report a case of brucella endocarditis on a native aortic valve causing severe aortic regurgitation, uncontrolled heart failure and disseminated intravascular coagulopathy. the diagnosis was proven by positive serology, isolation of brucella melitensis from preoperative blood cultures and excised valve. the patient was successfully treated with valve replacement and specific antibiotic therapy. | 1989 | 2681218 |
| acute pancreatitis associated with brucellosis. | to the best of our knowledge, no case of acute pancreatitis associated with brucellosis has been reported. this report details the occurrence of acute pancreatitis in which brucella melitensis was cultured from blood. we discuss various diagnostic tests and the recommended therapy for brucellosis. | 1989 | 2688402 |
| neurobrucellosis in children. | reports on nervous system involvement in brucellosis are rare in children. we report nine children with neurobrucellosis. the clinical presentation included meningitis in six patients, one with encephalitis, one with meningoencephalitis and one with meningomyeloencephalitis. the blood from all patients showed elevation in brucella microagglutination test titers (greater than or equal to 1:640) and in brucella-specific enzyme-linked immunosorbent assay for igm (greater than or equal to 1:800), ig ... | 1989 | 2704607 |
| use of hydrophobic cloths as antibody adsorbents for enzyme immunoassay: detection of brucella antigens. | a cloth-elisa (c-elisa) antigen capture assay for the detection of brucella melitensis and b. abortus was developed. segments (6-mm squares) of hydrophobic polyester cloth coated with diluted serum from a b. abortus-infected cow were incubated with saline suspensions of heat-killed brucella cells, or with cultures of bovine or sheep blood or bovine tissue homogenates that had been inoculated with b. abortus or b. melitensis added to trypticase soy broth (tsb) and incubated for 2-3 days. the capt ... | 1989 | 2505434 |
| humoral immunity in mice mediated by monoclonal antibodies against the a and m antigens of brucella. | all smooth strains of brucella bear two lipopolysaccharide (lps) antigens in a ratio that defines the classification of strains in serovars, a (a greater than m), m (m greater than a) and a.m (a = m). anti-lps-a monoclonal antibodies (mab-a) were previously shown to convey protection to mice against b. abortus (a) strain 544, as shown by lower spleen counts than in controls at days 7 and 21 after challenge. anti-lps-m monoclonal antibodies (mab-m) were obtained and tested for m-specificity with ... | 1989 | 2506351 |
| comparison of protein components of different species and strains of brucella. | the proteins constituents of brucella abortus, brucella melitensis and brucella ovis were analyzed sds-page. from the comparison appears that the three species of brucella studied shows a different electrophoretic pattern specially at the level of small peptides. on the contrary when two strains of b. abortus are analyzed no differences can be noticed. | 1989 | 2506718 |
| evaluation of humoral immunity to brucella sp in cattle by use of an agar-gel immunodiffusion test containing a polysaccharide antigen. | results of a double agar gel immunodiffusion (ouchterlony) test that contained a polysaccharide (poly-b) antigen of brucella melitensis strain b115 were compared with those of 5 other serotests. to determine the sensitivity and specificity of the immunodiffusion, standard tube, 2-mercaptoethanol, rivanol, card, and complement fixation tests, sera obtained from 1,328 vaccinated, infected, and seronegative cattle, 56 of which had been examined bacteriologically, were used to evaluate the humoral r ... | 1989 | 2515779 |
| pulmonary brucellosis. | pulmonary abnormalities in brucellosis are rare. we report on nine cases (five adults and four children) with pulmonary brucellosis. all presented with fever, cough and mucopurulent sputum, and most had abnormal signs in the chest. radiography of the chest showed pneumonic patches or consolidation in five patients, pleural effusion in three, granuloma of the lung in one and a picture of interstitial pneumonitis in one. all the patients had a brucella agglutination titre of 1:320 or more, and an ... | 1989 | 2594962 |
| antibacterial activity of norfloxacin against 1700 relatively resistant clinical isolates. | a total of 1724 clinical isolates from patients with different infections were tested in vitro to determine their susceptibility to norfloxacin. antibacterial activity of norfloxacin was compared with other drugs that were commonly used in the hospital. of the 919 strains of enterobacteriaceae tested, all except four isolates of enterobacter, two of serratia and one of proteus were susceptible to norfloxacin. ninety-five percent of the 199 strains of pseudomonas aeruginosa were also inhibited by ... | 1989 | 2598776 |
| enhancement of the antigen-binding capacity of incomplete igg antibodies to brucella melitensis through fc region interactions with staphylococcal protein a. | incomplete igg anti-brucella antibodies in human sera were detected using both a conventional elisa and a modified method. in the elisa procedure serum igg was allowed to bind solid-phase b. melitensis antigen and, after washing, biotinylated staphylococcal protein a (biospa) was used as an fc-specific tracer followed by streptavidin-hrp conjugate. in the modified method, serum igg was co-incubated with a defined quantity of biospa in the presence of solid-phase antigen. biospa bound fc regions ... | 1989 | 2600428 |
| influence of doxycycline, streptomycin, cotrimoxazole and rifamycin on the functions of normal human neutrophils against brucella melitensis. | 1989 | 16312472 | |
| review of pulmonary brucellosis: a case report on brucellar pulmonary empyema. | in certain geographic areas, among which most south european countries are included, brucellosis continues to be an important health problem. despite the disease's continued high prevalence, the advances made in its diagnosis and treatment have contributed to the rarity of different focal locations, such as the lung. formerly, these occurrences were much more frequent. the present work offers a review of the literature on pulmonary brucellar affection and describes a case report of brucellar pul ... | 1988 | 3064958 |
| a modified indirect fluorescent antibody test for the diagnosis of brucellosis. | we adapted the conventional indirect fluorescent antibody (ifa) test to assay igm and igg brucella-specific antibodies to differentiate acute from chronic infections rather than measure total antihuman globulin specific antibodies. the results were compared with the slide agglutination test (sat) used for screening and the quantitative microagglutination test (mat). of a total of 118 randomly selected samples sent for anti-brucella antibodies received at a general hospital laboratory, 58 (47.9%) ... | 1988 | 3071447 |
| brucella abortus 1119-3 o-chain polysaccharide to differentiate sera from b. abortus s-19-vaccinated and field-strain-infected cattle by agar gel immunodiffusion. | purified brucella abortus 1119-3 and brucella melitensis 16m lipopolysaccharide o-chain polysaccharides were not precipitated in agar gel immunodiffusion by any of 24 sera from vaccinated cattle but were precipitated by 18 of 24 sera from infected cattle. this difference can be used to differentiate sera of cattle vaccinated with b. abortus s-19 from sera of some field-strain-infected cattle. | 1988 | 3133389 |
| [100 years of bacteriology: the history of the discovery of brucellosis. 2: discovery of the etiology of infectious abortion in cattle by the danish veterinary pathologist bernhard bang and proof of the relation of "micrococcus melitensis" and and "bacillus abortus" by the american microbiologist alice evans]. | 1988 | 3136596 | |
| enzyme-linked immunosorbent assay for brucella melitensis-associated antigens. | an elisa assay was performed to detect antigens of brucella melitensis directly in the blood of patients affected by brucellosis. disposable polystyrene microtiter plates were coated with rabbit immunoglobulins anti-brucella melitensis antigens and then incubated with sera of brucellosis patients and sera of not infected normal subjects as a control, to standardize the conditions of the different steps of the assay. the level of the blood-containing brucella antigen bound to the plate was measur ... | 1988 | 3173124 |
| anti-chlamydial immunity in ewes conferred by vaccination with a combination of three live chlamydia, brucella and salmonella vaccines. | live attenuated vaccines against chlamydia psittaci var ovis, brucella melitensis and salmonella abortus ovis have previously been shown to be compatible in mice by subcutaneous administration. immunity against challenge with virulent chlamydia was, however, slightly decreased in associations including the b melitensis rev 1 vaccine. the chlamydia strain 1b vaccine was administered to four- to five-month-old female lambs, either alone or in combination with the b melitensis rev1 and the s abortu ... | 1988 | 3176252 |
| [sanitary and technologic evaluation of the rural processing of fresh goat cheese in chile]. | a sanitary and technological diagnosis of the goat cheese rural process was carried out. the purpose was to obtain more information for the planning of a program aimed to the improvement of this small agroindustry. samples of milk, curdle, dry abomasum, rennet, water and cheese of 10% of the small industries of two rural villages in two agricultural seasons, were taken. moreover, dilutions of the utensils and goat udders were prepared. the samples were subjected to microbiological analysis of me ... | 1988 | 3154301 |
| enzyme-linked immunosorbent assay with brucella native hapten polysaccharide and smooth lipopolysaccharide. | brucella melitensis native haptens (nh) are polysaccharides identical to the o-side chain of the smooth lipopolysaccharide (s-lps) (e. moreno, h. mayer, and i. moriyón, infect. immun. 55:2850-2853, 1987) which precipitate with sera from infected cattle but not from strain 19-vaccinated cattle. in the present work, nh was extracted by the hot-water method (r. díaz, j. toyos, m.d. salvo, and m.l. pardo, ann. rech. vet. 12:35-39, 1981) and purified free of s-lps and protein. purified nh lacked the ... | 1988 | 3147993 |
| elisa versus routine tests in the diagnosis of patients with systemic and neurobrucellosis. | sera from patients in different stages of brucellosis as well as sera and cerebrospinal fluid (csf) from patients with central nervous system (cns) brucellosis and controls, were tested by elisa for brucella-specific igg, igm and iga. the results were compared with culture findings, micro-agglutination (ma), slide agglutination with rose bengal (rb), and brucella melitensis stained antigens (sa). in sera of patients with acute brucellosis (296), elisa was positive for igm (100%), igg (97%) and i ... | 1988 | 3345262 |
| the characteristics of a variant strain of brucella melitensis rev 1. | circumstantial evidence is presented for the occurrence of a variant of a vaccine strain of b. melitensis rev 1, designated "fsa" (foreign south african). fsa resembles rev 1 in its reactions to penicillin and streptomycin but reacts closer to a field strain of b. melitensis as regards dye (thionine and basic fuchsin) sensitivity and colony size. although colonies of rev 1 were consistently smaller than other b. melitensis strains, their size was 0,75 mm as opposed to the 1-2 mm reported in the ... | 1988 | 3353094 |
| characterization of brucella polysaccharide b. | polysaccharide b was extracted from brucella melitensis 16m and from a rough strain of brucella abortus 45/20 by autoclaving or trichloroacetic acid extraction of whole cells and by a new method involving mild leaching of cells. the material obtained by either of the established procedures was contaminated by o polysaccharide. the new leaching protocol eliminated this impurity and provided a pure glucan, which was regarded as polysaccharide b. this polysaccharide was found by high-performance li ... | 1988 | 3356461 |
| recurrent epidermal cyst infection caused by brucella melitensis in a diabetic patient. | a presternal swelling diagnosed clinically as a sebaceous cyst in a 60-year-old diabetic patient was surgically drained, and the aspirated purulant material yielded growth of brucella melitensis. the swelling recurred four times and was drained on every occasion. the patient responded to surgical excision and antibrucella treatment. the histologic diagnosis was an epidermal cyst. | 1988 | 3384896 |
| probable transmission of brucellosis from breast milk to a newborn. | the first case of neonatal brucellosis is described. the baby was fed breast milk only when the mother became acutely ill with fever, arthralgia and malaise. at the same time the baby was febrile. blood cultures grew brucella melitensis in both mother and child. the brucella agglutination titre in the breast milk was 1:2560. both mother and child recovered during treatment with streptomycin. | 1988 | 3407006 |
| brucellar sternoclavicular arthritis, the forgotten complication. | of 511 cases of brucellosis studied between december 1983 and february 1986, four (0.8%) had sternoclavicular (stcl) arthritis. two were male and two female, and only one was younger than 50 years old. all four cases had significantly high specific igg antibody titres (1 of 1280), measured by the indirect immunofluorescent (iif) test, and two had brucella melitensis isolated from their blood. in two cases, stcl arthritis was the presenting problem, and it was associated in one with ankle arthrit ... | 1988 | 3250341 |
| polyclonal igg synthesis of mononuclear cells induced by the homopolymer of 4,6-dideoxy-4-formamido-alpha-d-mannopyranosyl units isolated from brucella melitensis. | brucella melitensis attaches selectively to b lymphocytes. the bacterium induces in vitro only a slight proliferation of mononuclear cells and inhibits the spontaneous igg synthesis. in order to characterize these effects, the o-chain of the lipopolysaccharide of brucella melitensis (a polysaccharide consisting of poly-4,6-dideoxy-4-formamido-alpha-d-mannoside) was prepared and its influence on lymphocyte functions tested. this compound had no effect on blast transformation whereas igg synthesis ... | 1988 | 3266469 |
| brucellosis in the upper nile region--melut district, sudan (report). | in the melut district, south sudan human and animal blood samples were investigated for brucella abortus and brucella melitensis. 9.2% of the investigated cattle and 16.2% of the green long-tailed monkies were brucella abortus-positive. school children revealed a 3.7% positivity rate, melut's hospital patients 5.2% and village children and adults 2.2%. however, only a small number of hospital patients was investigated. a small percentage of sheep and goats was investigated for brucella melitensi ... | 1988 | 3275090 |
| the isolation and serology of the "fsa" brucella melitensis rev. 1 mutant in a flock of sheep. | a flock of sheep, known to be infected with the "fsa" mutant of brucella melitensis rev. 1, was examined serologically and bacteriologically to determine whether any relationship existed which would help in the control of this infection in the field. an attempt was also made to determine whether vertical transmission occurred. twenty-one out of 62 sheep were bacteriologically positive. the best organs for isolation were the udder, supramammary lymphnodes and uterus. no significant relationship c ... | 1988 | 3194120 |
| acute hepatitis and brucella melitensis infection: clinicopathological findings. | the clinical and pathological findings in a patient who had acute hepatitis caused by brucella melitensis are described. antibiotic therapy induced a good clinical and biochemical response, although a relapse occurred. brucellosis must be considered in the differential diagnosis of pyrexia of unknown origin, particularly if associated hepatic involvement is present. a careful occupational and travel history is essential. | 1988 | 3259669 |
| [a rare cause of infection in chronic dialysis patients: malta fever (febris undulans melitensis)]. | a 45-years-old greek patient developed septicaemia in his 10th year of hemodialysis treatment. clinical investigation was directed first on bacterial infection of the arteriovenous shunt, on urosepsis or bronchopulmonary infection. then, serologically (widal's reaction and elisa-test) and in different blood-cultures brucella melitensis was detected as causative agent for malta fever (febris undulans). the focus of infection is suspected in unpasteurized sheep-milk cheese of mediterranean origine ... | 1988 | 3172682 |
| acute brucella ileitis. | human brucellosis is an enteric fever in which systemic symptoms are generally more prominent than gastrointestinal complaints. during an outbreak of infection caused by brucella melitensis, linked to ingestion of unpasteurized goat milk cheese, we treated a child with radiographic evidence of acute ileitis. alimentary tract involvement in brucellosis is reviewed. | 1988 | 3337066 |
| control of brucella melitensis brucellosis in developing countries. | 1987 | 3300717 | |
| control of brucella melitensis infection in sheep and goats--a review. | 1987 | 3307078 | |
| the structure of the lipopolysaccharide o-chain (m antigen) and polysaccharide b produced by brucella melitensis 16m. | the surface m antigen of brucella species has been identified as the lipopolysaccharide o-polysaccharide component composed of a repeating pentasaccharide unit containing a sequence of one 1,3- and four 1,2-linked, 4,6-dideoxy-4-formamido-alpha-d-mannopyranosyl units. a neutral polysaccharide produced by brucella species and referred to as polysaccharide b (poly b) has been identified as a family of circular 1,2-linked polymers of beta-d-glucopyranosyl units ranging in ring size from 17 to 24 gl ... | 1987 | 3582676 |
| sacroiliitis as a sole manifestation of brucella melitensis infection in a child. | a case of a 12-year-old boy with sacroiliitis documented by positive tc-99m mdp and ga-67 scans is described. isolation of brucella melitensis from the blood and bone marrow established the diagnosis. he responded promptly to docycycline therapy. throughout the course of his disease this boy had neither fever nor other signs of brucellosis, and x-ray was normal. | 1987 | 3595029 |
| immunization with brucella melitensis rev 1 against brucella ovis infection of rams. | the efficacy of brucella melitensis rev 1 vaccine (rev 1) for the prophylaxis of brucella ovis ram epididymitis was evaluated. twenty-nine 3-month-old rams were vaccinated with 2 x 10(9) rev 1 and 14 were revaccinated with 5 x 10(8) at 14 months of age. six rams remained unvaccinated as a control group. all rams were challenged with 5 x 10(8) b. ovis at 21 months of age. before being slaughtered 8 weeks later, only one vaccinated ram developed epididymitis while four of the six control rams deve ... | 1987 | 3686824 |
| structural elucidation of the brucella melitensis m antigen by high-resolution nmr at 500 mhz. | the brucella m antigen from the species type strain brucella melitensis 16m has been identified as a component of the cell wall lipopolysaccharide (lps). o polysaccharide liberated from this lps by mild acid hydrolysis exhibited m activity in serological tests and was shown to be a homopolymer of 4-formamido-4,6-dideoxy-alpha-d-mannopyranosyl residues arranged in an oligosaccharide repeating unit as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the native lipopolysacchar ... | 1987 | 3442684 |
| serological screenings of various infectious diseases on the cape verde islands (west africa). | general screening investigations with various antigens were carried out with a view to further specific investigations being carried out on the cape verde islands concerning infectious diseases. serological positive reactions were found in mumps, adeno, plt, cytomegaly, herpes, para-influenza 1, 2, 3, influenza a and b, mycoplasmosis, rs-virus, gonorrhoea, hepatitis a and b, r. conori, malaria, syphilis, brucella abortus, brucella melitensis, varicella, legionella, picornavirus, measles, german ... | 1987 | 3443744 |
| risk of infection by brucella melitensis for people living near infected goats. | taking advantage of a local outbreak of brucellosis in a herd of goats, we have assessed the risk of infection for people living nearby. by means of serological tests we have shown that such a risk is small provided that direct contact with contaminated tissues or secretions of goats is avoided. | 1987 | 3312421 |
| conjunctival vaccination of young goats with brucella melitensis strain rev 1. | three groups each of 15 four-month old female kids were vaccinated with brucella melitensis strain rev 1 either conjunctivally (1.1 x 10(7) or 1.1 x 10(9) cfu) or subcutaneously (1.1 x 10(9) cfu). one group of 15 kids remained unvaccinated as control. all kids were mated when 9.5 month old. three months later, they were challenged conjunctivally with b melitensis strain h38 at a dose of 5.1 x 10(6) cfu. blood samples collected before vaccination and throughout the experiment were subjected to ro ... | 1987 | 3451685 |
| [endocarditis with unusual causative agents]. | in the period 1947-1985, 601 patients with infective endocarditis were seen at the university hospital zurich and the kantonsspital lucerne. streptococci, enterococci and staphylococci were the predominant causative organisms in two-thirds of all cases. in more than 25% of the patients blood cultures remained negative. in 6 patients endocarditis was caused by very rare organisms, viz. coxiella burnetii (2 cases), hemophilus parainfluenzae, corynebacterium bovis (diphtheroids), brucella melitensi ... | 1987 | 3324331 |