Publications

multiplex pcr and microarray for detection of swine respiratory pathogens.	porcine respiratory disease complex (prdc) is one of the most important health concerns for pig producers and can involve multiple viral and bacterial pathogens. no simple, single-reaction diagnostic test currently exists for the simultaneous detection of major pathogens commonly associated with prdc. furthermore, the detection of most of the bacterial pathogens implicated in prdc currently requires time-consuming culture-based methods that can take several days to obtain results. in this study, ...	2015	26662640
non-saccharomyces yeasts protect against epithelial cell barrier disruption induced by salmonella enterica subsp. enterica serovar typhimurium.	the human gastrointestinal epithelium makes up the largest barrier separating the body from the external environment. whereas invasive pathogens cause epithelial barrier disruption, probiotic micro-organisms modulate tight junction regulation and improve epithelial barrier function. in addition, probiotic strains may be able to reduce epithelial barrier disruption caused by pathogenic species. the aim of this study was to explore non-saccharomyces yeast modulation of epithelial cell barrier func ...	2015	26280244
in vitro probiotic characterization of lactobacillus strains from fermented radish and their anti-adherence activity against enteric pathogens.	in this study, we evaluated the probiotic properties of lactobacillus plantarum, lactobacillus pentosus, and lactobacillus fermentum strains isolated from fermented radish. all the strains survived the simulated oro-gastrointestinal transit condition and showed significantly higher adherence to caco-2 cells compared with the probiotic strain lactobacillus rhamnosus gg. the strains showed broad-spectrum antimicrobial activity, autoaggregation, and coaggregation capacity with pathogens. furthermor ...	2015	26382558
determination of the thermal inactivation kinetics of listeria monocytogenes, salmonella enterica, and escherichia coli o157:h7 and non-o157 in buffer and a spinach homogenate.	produce has been associated with a rising number of foodborne illness outbreaks. while much produce is consumed raw, some is treated with mild heat, such as blanching or cooking. the objectives of this research were to compare the thermal inactivation kinetics of listeria monocytogenes, salmonella enterica, shiga toxin-producing escherichia coli (stec) o157:h7, and non-o157 stec in phosphate-buffered saline (pbs; ph 7.2) and a spinach homogenate and to provide an estimate of the safety of mild h ...	2015	26219359
pathogen enrichment device (ped) enables one-step growth, enrichment and separation of pathogen from food matrices for detection using bioanalytical platforms.	the bottleneck for accurate detection of foodborne pathogens is separation of target analytes from complex food matrices. currently used sample preparation methods are cumbersome, arduous and lengthy; thus, a user-friendly system is desirable. a hand-held sample preparation system designated pathogen enrichment device (ped) was built that contains a growth chamber, filters, and an ion exchange cartridge to deliver bacteria directly onto the detection platforms. escherichia coli o157:h7, salmonel ...	2015	26211638
biopreservative methods to control the growth of foodborne pathogens on fresh-cut lettuce.	fruits and vegetables can become contaminated by foodborne pathogens such as escherichia coli o157:h7, salmonella and listeria monocytogenes, and it has been demonstrated that current industrial sanitizing treatments do not eliminate the pathogens when present. chemical control is widely used, but biological control appears to be a better solution, mainly using the native microbiota present on fresh produce. the first objective of this study was to isolate native microbiota from whole and fresh- ...	2015	26210531
fundamental characteristics of deep-uv light-emitting diodes and their application to control foodborne pathogens.	low-pressure mercury uv (lp-uv) lamps have long been used for bacterial inactivation, but due to certain disadvantages, such as the possibility of mercury leakage, deep-uv-c light-emitting diodes (duv-leds) for disinfection have recently been of great interest as an alternative. therefore, in this study, we examined the basic spectral properties of duv-leds and the effects of uv-c irradiation for inactivating foodborne pathogens, including escherichia coli o157:h7, salmonella enterica serovar ty ...	2015	26162872
effect of milk fat content on the performance of ohmic heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium and listeria monocytogenes.	the effect of milk fat content on ohmic heating compared to conventional heating for inactivation of food-borne pathogens was investigated.	2015	26043029
comparative effects of ohmic and conventional heating for inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in skim milk and cream.	ohmic heating has proven advantages over conventional thermal processing and novel thermal alternative technologies. in this study, the effect of ohmic and conventional heating for pasteurizing skim milk and cream was examined. all treatment conditions for ohmic and conventional heating were identical except for composition of the heating chamber. in most cases, the reduction of three pathogens did not differ significantly between ohmic heating and conventional heating at fixed treatment tempera ...	2015	26038915
growth of salmonella enterica and listeria monocytogenes on fresh-cut cantaloupe under different temperature abuse scenarios.	effective cold chain management is a critical component of food safety practice. in this study, we examined the impact of commonly encountered temperature abuse scenarios on the proliferation of salmonella enterica and listeria monocytogenes on fresh-cut cantaloupe. inoculated fresh-cut cantaloupe cubes were subjected to various temperature abuse conditions, and the growth of s. enterica and l. monocytogenes was determined. during 1 week of storage, salmonella cell counts on fresh-cut cantaloupe ...	2015	26038902
synergistic effect of slightly acidic electrolyzed water and ultrasound at mild heat temperature in microbial reduction and shelf-life extension of fresh-cut bell pepper.	the objectives of this study were to evaluate the effect of combined treatments (slightly acidic electrolyzed water (saew), ultrasound (us), or mild heat (60°c)) on the growth of listeria monocytogenes and salmonella enterica serovar typhimurium in fresh-cut bell pepper, and the shelf-life and sensory quality (color and texture) were followed during storage at 4°c and 25°c. an additional 0.65, 1.72, and 2.70 log cfu/g reduction was achieved by heat treatments at 60°c for 1 min for dw, saew, and ...	2015	26032362
inactivation dynamics of salmonella enterica, listeria monocytogenes, and escherichia coli o157:h7 in wash water during simulated chlorine depletion and replenishment processes.	maintaining effective sanitizer concentration is of critical importance for preventing pathogen survival and transference during fresh-cut produce wash operation and for ensuring the safety of finished products. however, maintaining an adequate level of sanitizer in wash water can be challenging for processors due to the large organic load in the wash system. in this study, we investigated how the survival of human pathogens was affected by the dynamic changes in water quality during chlorine de ...	2015	25998820
ultrasensitive detection and rapid identification of multiple foodborne pathogens with the naked eyes.	in this study, a novel approach for ultrasensitive detection and rapid high-throughput identification of a panel of common foodborne pathogens with the naked eyes is presented. as a proof-of-concept application, a multiple pathogen analysis array is fabricated through immobilizing three specific polyt-capture probes which can respectively recognize rfbe gene (escherichia coli o157:h7), inva gene (salmonella enterica), inla gene (listeria monocytogenes) on the plastic substrates. pcr has been dev ...	2015	25909338
multiplexed detection of foodborne pathogens based on magnetic particles.	this paper addresses the novel approaches for the multiplex detection of food poisoning bacteria, paying closer attention to three of the most common pathogens involved in food outbreaks: salmonella enterica, escherichia coli o157:h7 and listeria monocytogenes. end-point and real-time pcr, classical immunological techniques, biosensors, microarrays and microfluidic platforms, as well as commercial kits for multiplex detection of food pathogens will be reviewed, with special focus on the role of ...	2015	25858812
effect of sulfur dioxide fumigation on survival of foodborne pathogens on table grapes under standard storage temperature.	we examined the fate of listeria monocytogenes, escherichia coli o157:h7, and salmonella enterica thompson inoculated on freshly-harvested table grapes under standard cold storage with initial and weekly sulfur dioxide (so2) fumigation. l. monocytogenes and s. enterica thompson were much more sensitive to cold temperature than e. coli o157:h7. furthermore, l. monocytogenes was highly susceptible to so2. initial fumigation with 100 or 200 ppm-hr was sufficient to eliminate this pathogen on grapes ...	2015	25846930
growth inhibition of listeria monocytogenes, salmonella enterica, and escherichia coli o157:h7 by d-tryptophan as an incompatible solute.	under osmotic stress, bacterial cells uptake compatible solutes such as glycine-betaine to maintain homeostasis. it is unknown whether incompatible solutes exist that are similar in structure to compatible solutes but have adverse physiological effects on bacterial physiology. the objective of this study was to evaluate solute incompatibility of various amino acids against bacterial growth. bacterial growth was evaluated by changes in optical density at 595 nm in peptone-yeast-glucose (pyg) brot ...	2015	25836411
evaluation of novel micronized encapsulated essential oil-containing phosphate and lactate blends for growth inhibition of listeria monocytogenes and salmonella on poultry bologna, pork ham, and roast beef ready-to-eat deli loaves.	essential oils and their constituents are reported to possess potent antimicrobial activity, but their use in food processing is limited because of low solubility in aqueous systems and volatilization during processing. two proprietary noncommercial essential oil-containing phosphate blends were evaluated for antimicrobial activity against salmonella enterica cocktail (sc)-and listeria monocytogenes (lm)-inoculated deli meat products made from pork, poultry, or beef. four treatments were tested ...	2015	25836394
previous physicochemical stress exposures influence subsequent resistance of escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes to ultraviolet-c in coconut liquid endosperm beverage.	this study investigated the influences of prior exposures to common physicochemical stresses encountered by microorganisms in food and food processing ecologies such as acidity, desiccation, and their combinations, on their subsequent susceptibility towards uv-c treatment in coconut liquid endosperm beverage. cocktails of escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes were separately subjected to gradually acidifying environment (final ph 4.46), exposed to abrupt desic ...	2015	25723813
interaction of graphene family materials with listeria monocytogenes and salmonella enterica.	graphene family materials have unique properties, which make them valuable for a range of applications. the antibacterial properties of graphene have been reported; however, findings have been contradictory. this study reports on the antimicrobial proprieties of three different graphene materials (pristine graphene (pg), graphene oxide (go), and reduced graphene oxide (rgo)) against the food-borne bacterial pathogens listeria monocytogenes and salmonella enterica. a high concentration (250 μg/ml ...	2015	25685114
risk-based control of food-borne pathogens listeria monocytogenes and salmonella enterica in the italian fermented sausages cacciatore and felino.	fermentation is the most important killing step during production of fermented meats to eliminate food-borne pathogens. the objective was to evaluate whether the food-borne pathogens listeria monocytogenes and salmonella enterica may survive during the production of two italian fermented sausages. sausage batter was inoculated with five strains of l. monocytogenes or s. enterica (ca. 10(5)-10(6) cfu/g) and their kinetic behavior was monitored during production. both pathogens survived relatively ...	2015	25612557
transcriptional response of selected genes of salmonella enterica serovar typhimurium biofilm cells during inactivation by superheated steam.	superheated steam (shs), produced by the addition of heat to saturated steam (ss) at the same pressure, has great advantages over conventional heat sterilization due to its high temperature and accelerated drying rate. we previously demonstrated that treatment with shs at 200°c for 10 sec inactivated escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes biofilm cells on the surface of stainless steel to below the detection limit. however, bacteria withstanding heat stress ...	2015	25440555
subversion of the cytoskeleton by intracellular bacteria: lessons from listeria, salmonella and vibrio.	entry into host cells and intracellular persistence by invasive bacteria are tightly coupled to the ability of the bacterium to disrupt the eukaryotic cytoskeletal machinery. herein we review the main strategies used by three intracellular pathogens to harness key modulators of the cytoskeleton. two of these bacteria, namely listeria monocytogenes and salmonella enterica serovar typhimurium, exhibit quite distinct intracellular lifestyles and therefore provide a comprehensive panel for the under ...	2015	25440316
development of a multi-pathogen enrichment broth for simultaneous growth of five common foodborne pathogens.	the objective of the present study was to formulate a multi-pathogen enrichment broth which could support the simultaneous growth of five common foodborne pathogens (salmonella enterica, staphylococcus aureus, shigella flexneri, listeria monocytogenes and escherichia coli o157:h7). the formulated broth sssle was composed of potassium tellurite, bile salt, lithium chloride, and sodium chloride as growth-inhibitors; glucose, esculin, mannitol and sodium pyruvate as growth-promoters. compared with ...	2015	26782652
world health organization estimates of the global and regional disease burden of 22 foodborne bacterial, protozoal, and viral diseases, 2010: a data synthesis.	foodborne diseases are important worldwide, resulting in considerable morbidity and mortality. to our knowledge, we present the first global and regional estimates of the disease burden of the most important foodborne bacterial, protozoal, and viral diseases.	2015	26633831
murine gammaherpesvirus 68 pathogenesis is independent of caspase-1 and caspase-11 in mice and impairs interleukin-1β production upon extrinsic stimulation in culture.	gammaherpesviruses establish lifelong infections that are associated with the development of cancer. these viruses subvert many aspects of the innate and adaptive immune response of the host. the inflammasome, a macromolecular protein complex that controls inflammatory responses to intracellular danger signals generated by pathogens, is both activated and subverted during human gammaherpesvirus infection in culture. the impact of the inflammasome response on gammaherpesvirus replication and late ...	2015	25855746
growth and inactivation of salmonella enterica and listeria monocytogenes in broth and validation in ground pork meat during simulated home storage abusive temperature and home pan-frying.	ground pork meat with natural microbiota and inoculated with low initial densities (1-10 or 10-100 cfu/g) of salmonella enterica or listeria monocytogenes was stored under abusive temperature at 10°c and thermally treated by a simulated home pan-frying procedure. the growth and inactivation characteristics were also evaluated in broth. in ground pork meat, the population of s. enterica increased by less than one log after 12-days of storage at 10°c, whereas l. monocytogenes increased by 2.3 to 2 ...	2015	26579079
pectin and xyloglucan influence the attachment of salmonella enterica and listeria monocytogenes to bacterial cellulose-derived plant cell wall models.	minimally processed fresh produce has been implicated as a major source of foodborne microbial pathogens globally. these pathogens must attach to the produce in order to be transmitted. cut surfaces of produce that expose cell walls are particularly vulnerable. little is known about the roles that different structural components (cellulose, pectin, and xyloglucan) of plant cell walls play in the attachment of foodborne bacterial pathogens. using bacterial cellulose-derived plant cell wall models ...	2015	26567310
using uvc light-emitting diodes at wavelengths of 266 to 279 nanometers to inactivate foodborne pathogens and pasteurize sliced cheese.	uvc light is a widely used sterilization technology. however, uv lamps have several limitations, including low activity at refrigeration temperatures, a long warm-up time, and risk of mercury exposure. uv-type lamps only emit light at 254 nm, so as an alternative, uv light-emitting diodes (uv-leds) which can produce the desired wavelengths have been developed. in this study, we validated the inactivation efficacy of uv-leds by wavelength and compared the results to those of conventional uv lamps ...	2015	26386061
effect of alginate coatings with cinnamon bark oil and soybean oil on quality and microbiological safety of cantaloupe.	the quality and microbiological safety of cantaloupes can potentially be improved using antimicrobial coatings that are able to maintain effectiveness throughout storage. the objective of this work was to study the effect of coating mixtures containing sodium alginate and cinnamon bark oil (cbo) on the quality of cantaloupes and the survival of inoculated bacterial pathogens and naturally occurring yeasts and molds during ambient storage at 21 °c. cantaloupes were dipped in mixtures containing 1 ...	2015	26318410
improved antimicrobial activities of synthetic-hybrid bacteriocins designed from enterocin e50-52 and pediocin pa-1.	two hybrid bacteriocins, enterocin e50-52/pediocin pa-1 (ep) and pediocin pa-1/enterocin e50-52 (pe), were designed by combining the n terminus of enterocin e50-52 and the c terminus of pediocin pa-1 and by combining the c terminus of pediocin pa-1 and the n terminus of enterocin e50-52, respectively. both hybrid bacteriocins showed reduced mics compared to those of their natural counterparts. the mics of hybrid pe and ep were 64- and 32-fold lower, respectively, than the mic of pediocin pa-1 an ...	2015	25527560
modulation of systemic and mucosal immunity against an inactivated vaccine of newcastle disease virus by oral co-administration of live attenuated salmonella enterica serovar typhimurium expressing chicken interleukin-18 and interferon-α.	newcastle disease (nd) is a highly contagious disease of chickens causing significant economic losses worldwide. due to limitations in the efficacy against currently circulating nd viruses, existing vaccination strategies require improvements, and incorporating immunomodulatory cytokines with existing vaccines might be a novel approach. here, we investigated the systemic and mucosal immunomodulatory properties of oral co-administration of chicken interleukin-18 (chil-18) and chicken interferon-α ...	2015	25502364
high-throughput crispr typing of mycobacterium tuberculosis complex and salmonella enterica serotype typhimurium.	spoligotyping was developed almost 18 years ago and still remains a popular first-lane genotyping technique to identify and subtype mycobacterium tuberculosis complex (mtc) clinical isolates at a phylogeographic level. for other pathogens, such as salmonella enterica, recent studies suggest that specifically designed spoligotyping techniques could be interesting for public health purposes. spoligotyping was in its original format a reverse line-blot hybridization method using capture probes desi ...	2015	25981468
solution structure and functional studies of the highly potent equine antimicrobial peptide defa1.	defensins are small effector molecules of the innate immune system that are present in almost all organisms including plants and animals. these peptides possess antimicrobial activity against a broad range of microbes including bacteria, fungi and viruses and act as endogenous antibiotics. α-defensins are a subfamily of the defensin family and their expression is limited to specific tissues. equine defa1 is an enteric α-defensin exclusively secreted by paneth cells and shows an activity against ...	2015	25769951
enhanced inactivation of food-borne pathogens in ready-to-eat sliced ham by near-infrared heating combined with uv-c irradiation and mechanism of the synergistic bactericidal action.	the objective of the study described in this article was, first, to investigate the effect of the simultaneous application of near-infrared (nir) heating and uv irradiation on inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in ready-to-eat (rte) sliced ham and as well as its effect on product quality and, second, to elucidate the underlying mechanisms of the synergistic bactericidal action of nir heating and uv irradiation. with the i ...	2015	25107964
the n terminus of type iii secretion needle protein yscf from yersinia pestis functions to modulate innate immune responses.	the type iii secretion system is employed by many pathogens, including the genera yersinia, shigella, pseudomonas, and salmonella, to deliver effector proteins into eukaryotic cells. the injectisome needle is formed by the polymerization of a single protein, e.g., yscf (yersinia pestis), pscf (pseudomonas aeruginosa), prgi (salmonella enterica spi-1), ssag (salmonella enterica spi-2), or mxih (shigella flexneri). in this study, we demonstrated that the n termini of some needle proteins, particul ...	2015	25644012
di-icr-ft-ms-based high-throughput deep metabotyping: a case study of the caenorhabditis elegans-pseudomonas aeruginosa infection model.	in metabolomics there is an ever-growing need for faster and more comprehensive analysis methods to cope with the increasing size of biological studies. direct-infusion ion-cyclotron-resonance fourier-transform spectrometry (di-icr-ft-ms) is used in non-targeted metabolomics to obtain high-resolution snapshots of the metabolic state of a system. we applied this technology to a caenorhabditis elegans-pseudomonas aeruginosa infection model and optimized times needed for cultivation and mass-spectr ...	2015	25428456
two novel salmonella genomic island 1 variants in proteus mirabilis isolates from swine farms in china.	four different salmonella genomic island 1 (sgi1) variants, including two novel variants, were characterized in one salmonella enterica serovar rissen sequence type st1917 isolate and three proteus mirabilis isolates from swine farms in china. one novel variant was derived from sgi1-b with the backbone gene s021 disrupted by a 12.72-kb is26 composite transposon containing the dfra17-aada5 cassettes and macrolide inactivation gene cluster mpha-mrx-mphr. the other one was an integron-free sgi1 and ...	2015	25918148
binding interface between the salmonella σ(s)/rpos subunit of rna polymerase and crl: hints from bacterial species lacking crl.	in many gram-negative bacteria, including salmonella enterica serovar typhimurium (s. typhimurium), the sigma factor rpos/σ(s) accumulates during stationary phase of growth, and associates with the core rna polymerase enzyme (e) to promote transcription initiation of genes involved in general stress resistance and starvation survival. whereas σ factors are usually inactivated upon interaction with anti-σ proteins, σ(s) binding to the crl protein increases σ(s) activity by favouring its associati ...	2015	26338235
creation of a putative third metal binding site in type ii dihydroorotases significantly enhances enzyme activity.	dihydroorotase (dhoase) is the third enzyme in the de novo biosynthesis pathway of pyrimidine nucleotides. dhoase is divided into two types (i and ii). type ii dhoase generally contains a binuclear metal center in its active site. recently, the crystal structure of dhoase domain in human cad protein (hudhoase) has revealed three metal ions in the protein's active site. however, whether type ii dhoase can have the critical third metal ion, as observed in hudhoase, remains unknown. in the present ...	2015	26446564
induction of the sugar-phosphate stress response allows saccharomyces cerevisiae 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate synthase to function in salmonella enterica.	thiamine pyrophosphate is a required cofactor for all forms of life. the pyrimidine moiety of thiamine, 2-methyl-4-amino-5-hydroxymethylpyrimidine phosphate (hmp-p), is synthesized by different mechanisms in bacteria and plants compared to fungi. in this study, salmonella enterica was used as a host to probe requirements for activity of the yeast hmp-p synthase, thi5p. thi5p synthesizes hmp-p from histidine and pyridoxal-5-phosphate and was reported to use a backbone histidine as the substrate, ...	2015	26324451
assessment of anti-salmonella activity of boot dip samples.	the introduction of pathogens from the external environment into poultry houses via the boots of farm workers and visitors presents a significant risk. the use of boot dips containing disinfectant to help prevent this from happening is common practice, but the effectiveness of these boot dips as a preventive measure can vary. the aim of this study was to assess the anti-salmonella activity of boot dips that are being used on poultry farms. boot dip samples were collected from commercial laying h ...	2015	25650744
dietary β-galactomannans have beneficial effects on the intestinal morphology of chickens challenged with salmonella enterica serovar enteritidis.	salmonella enterica serovar enteritidis is one of the leading causes of food-borne salmonellosis in humans. poultry is the single largest reservoir, and the consumption of incorrectly processed chicken meat and egg products is the major source of infection. since 2006, the use of antibiotics as growth promoters has been banned in the european union, and the dietary inclusion of β-galactomannans (βgm) has become a promising strategy to control and prevent intestinal infections. the aim of this st ...	2015	25568372
perp, a host tetraspanning membrane protein, is required for salmonella-induced inflammation.	salmonella enterica typhimurium induces intestinal inflammation through the activity of type iii secreted effector (t3se) proteins. our prior results indicate that the secretion of the t3se sipa and the ability of sipa to induce epithelial cell responses that lead to induction of polymorphonuclear transepithelial migration are not coupled to its direct delivery into epithelial cells from salmonella. we therefore tested the hypothesis that sipa interacts with a membrane protein located at the api ...	2015	25486861
in salmonella enterica, the gcn5-related acetyltransferase mdda (formerly ynca) acetylates methionine sulfoximine and methionine sulfone, blocking their toxic effects.	protein and small-molecule acylation reactions are widespread in nature. many of the enzymes catalyzing acylation reactions belong to the gcn5-related n-acetyltransferase (gnat; pf00583) family, named after the yeast gcn5 protein. the genome of salmonella enterica serovar typhimurium lt2 encodes 26 gnats, 11 of which have no known physiological role. here, we provide in vivo and in vitro evidence for the role of the mdda (methionine derivative detoxifier; formerly ynca) gnat in the detoxificatio ...	2015	25368301
the m3 muscarinic receptor is required for optimal adaptive immunity to helminth and bacterial infection.	innate immunity is regulated by cholinergic signalling through nicotinic acetylcholine receptors. we show here that signalling through the m3 muscarinic acetylcholine receptor (m3r) plays an important role in adaptive immunity to both nippostrongylus brasiliensis and salmonella enterica serovar typhimurium, as m3r-/- mice were impaired in their ability to resolve infection with either pathogen. cd4 t cell activation and cytokine production were reduced in m3r-/- mice. immunity to secondary infec ...	2015	25629518
oligosaccharide structure determines prebiotic role of β-galactomannan against salmonella enterica ser. typhimurium in vitro.	prebiotics and probiotics are considered natural alternatives to dietary antibiotics in animal production. plant extracts and yeast cell walls are mannose-rich products that can be used as substrate for adhesion of gram-negative bacteria. we assessed whether the structure of these saccharides is relevant to develop their role as prebiotics and therefore, their suitability to be used as alternatives to antibiotics to prevent intestinal infections in pigs. the prebiotic functionality of β-galactom ...	2015	23137964
molecular detection of fastidious and common bacteria as well as plasmodium spp. in febrile and afebrile children in franceville, gabon.	malaria was considered as the main cause of fever in africa. however, with the roll back malaria initiative, the causes of fever in africa may change. this study aimed to evaluate the prevalence of bacteria and plasmodium spp. in febrile and afebrile (controls) children from franceville, gabon. about 793 blood samples from febrile children and 100 from controls were analyzed using polymerase chain reaction (pcr) coupled with sequencing. plasmodium spp. was the microorganism most detected in febr ...	2015	25802432
optical imaging of paramagnetic bead-dna aggregation inhibition allows for low copy number detection of infectious pathogens.	dna-paramagnetic silica bead aggregation in a rotating magnetic field facilitates the quantification of dna with femtogram sensitivity, but yields no sequence-specific information. here we provide an original description of aggregation inhibition for the detection of dna and rna in a sequence-specific manner following loop-mediated isothermal amplification (lamp). the fragments generated via lamp fail to induce chaotrope-mediated bead aggregation; however, due to their ability to passivate the b ...	2015	26068926
contribution to virulence of a novel anti-inflammatory effector in salmonella enterica.		2015	27437422
convergent synthesis of oligosaccharide fragments corresponding to the cell wall o-polysaccharide of salmonella enterica o53.	conventional glycoconjugate vaccines are prepared with polysaccharides isolated from bacterial fermentation, an approach with some significant drawbacks such as handling of live bacterial strains, the presence of biological impurities, and inter-batch variations in oligosaccharide epitope structure. however, it has been shown in many cases that a synthetic fragment of appropriate structure conjugated to a protein can be an effective vaccine that circumvents the shortcomings of using full-length ...	2015	27308202
early onset neonatal sepsis due to salmonella enterica serovar 4,5,12:i:-: a case report with literature review.	the authors report a case of a 36-week male infant born via spontaneous vaginal delivery who developed salmonella sepsis at hrh princess maha chakri sirindhorn medical center srinakharinwirot university, nakhon nayok, thailand. he was born to a mother without identifiable risk factors. on day 3, he developed fever tachycardia, lethargy, poor feeding and diarrhea prompting a sepsis evaluation. blood and stool cultures were positive for s. enterica serovar 4,5,12:i:-. therefore, salmonella infecti ...	2015	27276847
prevalence and epidemiology of salmonella enterica serovar gallinarum from poultry in some parts of haryana, india.	the present study was investigated to ascertain the epidemiological status of fowl typhoid (ft) in broilers in some parts of haryana during january 2011 to december 2013.	2015	27047033
[fluorescent and magnetic relaxation switch immunosensor for the detecting foodborne pathogen salmonella enterica in water samples].	fluoroimmunoassay based on quantum dots (qds) and magnetic relaxation switch (mrs) immunoassay based on superparamagnetic nanoparticles (smn) were constructed to detect salmonella enterica (s. enterica) in water samples. in fluoroimmunoassay, magnetic beads was conjugated with s. enterica capture antibody (mb-ab2) to enrich s. enterica from sample solution, then the qds was conjugated with the s. enterica detection antibody (qds-ab1) to detect s. enterica based on sandwich immunoassay format. an ...	2015	26978918
salmonella enterica paratyphi a infections in travelers returning from cambodia, united states.		2015	25988984
salmonella enterica serotype enteritidis in french polynesia, south pacific, 2008-2013.	outbreaks of salmonella enterica serotype enteritidis infections associated with eggs occurred in french polynesia during 2008-2013. molecular analysis of isolates by using clustered regularly interspaced short palindromic repeat polymorphisms and multilocus variable-number tandem-repeat analysis was performed. this subtyping made defining the epidemic strain, finding the source, and decontaminating affected poultry flocks possible.	2015	25988406
importance of sigma factor mutations in increased triclosan resistance in salmonella typhimurium.	salmonella enterica is the second most common foodborne pathogen. the use of biocides is crucial to prevent spread of foodborne pathogens, and it would be devastating for food safety if salmonella would become resistant to the disinfectants used. another concern is that exposure to disinfectants might lead to decreased susceptibility to antibiotics. the current study aimed to identify genetic changes causing high level triclosan resistance in s. enterica serovar typhimurium and evaluate how thes ...	2015	25986727
absence of multidrug resistance in salmonella enterica serotypes typhi and paratyphi a isolates with intermediate susceptibility to ciprofloxacin.	we describe the antimicrobial susceptibility pattern of 64 blood stream isolates of salmonella enterica serotypes typhi and paratyphi a studied from january 2013 to december 2014 at a tertiary care centre in north india.	2015	25979527
genomic and experimental evidence for multiple metabolic functions in the rida/yjgf/yer057c/uk114 (rid) protein family.	it is now recognized that enzymatic or chemical side-reactions can convert normal metabolites to useless or toxic ones and that a suite of enzymes exists to mitigate such metabolite damage. examples are the reactive imine/enamine intermediates produced by threonine dehydratase, which damage the pyridoxal 5'-phosphate cofactor of various enzymes causing inactivation. this damage is pre-empted by rida proteins, which hydrolyze the imines before they do harm. rida proteins belong to the yjgf/yer057 ...	2015	25975565
trends in antibiotic susceptibility of enteric fever isolates in east london.	the study sought evidence for changes in the proportions of antibiotic resistant strains among isolates of salmonella enterica serovar typhi (s. typhi) and salmonella enterica serovar paratyphi (s. paratyphi) between 2005 and 2012.	2015	25964222
antimicrobial susceptibility of travel-related salmonella enterica serovar typhi isolates detected in switzerland (2002-2013) and molecular characterization of quinolone resistant isolates.	typhoid fever is an acute, invasive, and potentially fatal systemic infection caused by salmonella enterica subspecies enterica serotype typhi (s. typhi). drug resistance to antimicrobials such as ciprofloxacin is emerging in developing countries, threatening the efficacy of treatment of patients in endemic regions as well as of travellers returning from these countries.	2015	25963025
the pdul phosphotransacylase is used to recycle coenzyme a within the pdu microcompartment.	in salmonella enterica, 1,2-propanediol (1,2-pd) utilization (pdu) is mediated by a bacterial microcompartment (mcp). the pdu mcp consists of a multiprotein shell that encapsulates enzymes and cofactors for 1,2-pd catabolism, and its role is to sequester a reactive intermediate (propionaldehyde) to minimize cellular toxicity and dna damage. for the pdu mcp to function, the enzymes encapsulated within must be provided with a steady supply of substrates and cofactors. in the present study, western ...	2015	25962918
in salmonella enterica, ethanolamine utilization is repressed by 1,2-propanediol to prevent detrimental mixing of components of two different bacterial microcompartments.	bacterial microcompartments (mcps) are a diverse family of protein-based organelles composed of metabolic enzymes encapsulated within a protein shell. the function of bacterial mcps is to optimize metabolic pathways by confining toxic and/or volatile metabolic intermediates. about 20% of bacteria produce mcps, and there are at least seven different types. different mcps vary in their encapsulated enzymes, but all have outer shells composed of highly conserved proteins containing bacterial microc ...	2015	25962913
phylogeographical analysis of the dominant multidrug-resistant h58 clade of salmonella typhi identifies inter- and intracontinental transmission events.	the emergence of multidrug-resistant (mdr) typhoid is a major global health threat affecting many countries where the disease is endemic. here whole-genome sequence analysis of 1,832 salmonella enterica serovar typhi (s. typhi) identifies a single dominant mdr lineage, h58, that has emerged and spread throughout asia and africa over the last 30 years. our analysis identifies numerous transmissions of h58, including multiple transfers from asia to africa and an ongoing, unrecognized mdr epidemic ...	2015	25961941
mechanisms of antimicrobial resistant salmonella enterica transmission associated with starling-livestock interactions.	bird-livestock interactions have been implicated as potential sources for bacteria within concentrated animal feeding operations (cafo). european starlings (sturnus vulgaris) in particular are known to contaminate cattle feed and water with salmonella enterica through their fecal waste. we propose that fecal waste is not the only mechanisms through which starlings introduce s. enterica to cafo. the goal of this study was to assess if starlings can mechanically move s. enterica. we define mechani ...	2015	25960334
transcriptional and post-transcriptional modulation of spi1 and spi2 expression by ppgpp, rpos and dksa in salmonella enterica sv typhimurium.	the expression of genes within salmonella pathogenicity islands 1 and 2 (spi1, spi2) is required to facilitate invasion and intracellular replication respectively of s. typhimurium in host cell lines. control of their expression is complex and occurs via a variety of factors operating at transcriptional and post-transcriptional levels in response to the environmental stimuli found within the host. several of the factors that modulate spi1 and spi2 expression are involved in the redistribution or ...	2015	26039089
genomic comparison of the closely-related salmonella enterica serovars enteritidis, dublin and gallinarum.	the salmonella enterica serovars enteritidis, dublin, and gallinarum are closely related but differ in virulence and host range. to identify the genetic elements responsible for these differences and to better understand how these serovars are evolving, we sequenced the genomes of enteritidis strain lk5 and dublin strain sarb12 and compared these genomes to the publicly available enteritidis p125109, dublin ct 02021853 and dublin sd3246 genome sequences. we also compared the publicly available g ...	2015	26039056
improvements to a pcr-based serogrouping scheme for salmonella enterica from dairy farm samples.	molecular serotyping through the use of pcr is a simple and useful technique for characterizing isolates of salmonella enterica subsp. enterica belonging to serogroups b, c1, c2, d1, and e1, which are the majority of the isolates associated with human disease outbreaks. however, many of the salmonella strains currently isolated from dairy farms in the northeastern united states are serovar cerro, a group k strain not detected by this assay. primers from a well-known pcr assay for the identificat ...	2015	26038910
genetic and phenotypic evidence of the salmonella enterica serotype enteritidis human-animal interface in chile.	salmonella enterica serotype enteritidis is a worldwide zoonotic agent that has been recognized as a very important food-borne bacterial pathogen, mainly associated with consumption of poultry products. the aim of this work was to determine genotypic and phenotypic evidence of s. enteritidis transmission among seabirds, poultry and humans in chile. genotyping was performed using pcr-based virulotyping, pulse-field gel electrophoresis (pfge) and multi-locus sequence typing (mlst). pathogenicity-a ...	2015	26029196
salmonella manipulation of host signaling pathways provokes cellular transformation associated with gallbladder carcinoma.	cancer is fueled by deregulation of signaling pathways in control of cellular growth and proliferation. these pathways are also targeted by infectious pathogens en route to establishing infection. gallbladder carcinoma (gbc) is frequent in the indian subcontinent, with chronic salmonella enterica serovar typhi infection reported as a significant risk factor. however, direct association and causal mechanisms between salmonella typhi infection and gbc have not been established. deconstructing the ...	2015	26028364
efficient suppression of biofilm formation by a nucleic acid aptamer.	biofilms are microbial communities that are attached to a solid surface using extracellular polymeric substances. motility and initial attachment mediated by flagella are required for biofilm formation. therefore, blocking the motility of flagella is a potential strategy to inhibit biofilm formation. in this study, single-stranded dna aptamers specific to the salmonella choleraesuis were selected after 14 cycles of the systematic evolution of ligands by exponential enrichment. among the selected ...	2015	26025307
massive lineage replacements and cryptic outbreaks of salmonella typhi in eastern and southern africa.	a new large-scale study reports the whole-genome sequences of nearly 2,000 salmonella enterica serovar typhi (s. typhi) strains collected from 63 countries. a recent and dominant multidrug-resistant (mdr) lineage originating from south asia, which is supplanting a bulk of ancestral drug-sensitive strains, is identified; the success of this lineage is likely driven by plasmid acquisitions and the chromosomal integration of resistance-conferring genes.	2015	26018894
[salmonella meningitis in an infant due to a pet turtle].	in humans, salmonella most often causes self-limiting gastroenteritis, but more severe symptoms such as sepsis and meningitis can also occur and can sometimes have a fatal outcome. even if the meningitis is not fatal, sequelae such as epilepsy, cranial nerve palsies, and hydrocephalus can occur. in the united states, it has been estimated that approximately 6% of the human cases of salmonellosis can be attributed to contact with reptiles or amphibians. the infection may take place by direct cont ...	2015	26014646
acidic ph and divalent cation sensing by phoq are dispensable for systemic salmonellae virulence.	salmonella phoq is a histidine kinase with a periplasmic sensor domain (pd) that promotes virulence by detecting the macrophage phagosome. phoq activity is repressed by divalent cations and induced in environments of acidic ph, limited divalent cations, and cationic antimicrobial peptides (camp). previously, it was unclear which signals are sensed by salmonellae to promote phoq-mediated virulence. we defined conformational changes produced in the phoq pd on exposure to acidic ph that indicate st ...	2015	26002083
altered host immune responses to membrane vesicles from salmonella and gram-negative pathogens.	membrane vesicles (mvs), discrete nano-structures produced from the outer membrane of gram-negative bacteria such as salmonella enterica typhimurium (s. typhimurium), strongly activate dendritic cells (dcs), contain major antigens (ags) recognized by salmonella-specific b-cells and cd4+ t-cells, and provide protection against s. typhimurium challenge in a mouse model. with this in mind, we hypothesized that alterations to the gene expression profile of bacteria will be reflected in the immunolog ...	2015	26001432
effectiveness of levulinic acid and sodium dodecyl sulfate employed as a sanitizer during harvest or packing of cantaloupes contaminated with salmonella poona.	freshly harvested eastern variety cantaloupes (cucumis melo l. var. reticulatus cv. athena) were subjected to three different harvest and wash treatments to examine conditions under which the efficacy of the sanitizer, levulinic acid (lv) plus sodium dodecyl sulfate (sds), could be enhanced to reduce salmonella contamination. in treatment set one, cantaloupes were spot inoculated with salmonella enterica serovar poona (prepared from solid or liquid media cultures) before or after a 1-min dip tre ...	2015	26001062
inactivation of escherichia coli o157:h7 and salmonella enterica on blueberries in water using ultraviolet light.	ultraviolet light (uv) has antimicrobial effects, but the shadowing effect has limited its application. in this study, a novel setup using uv processing in agitated water was developed to inactivate escherichia coli o157:h7 and salmonella on blueberries. blueberries were dip- or spot-inoculated with e. coli or salmonella. blueberries inoculated with e. coli were treated for 2 to 10 min with uv directly (dry uv) or immersed in agitated water during uv treatment (wet uv). e. coli was most easily k ...	2015	25998253
immune reaction and survivability of salmonella typhimurium and salmonella infantis after infection of primary avian macrophages.	salmonella serovars are differentially able to infect chickens. the underlying causes are not yet fully understood. aim of the present study was to elucidate the importance of salmonella pathogenicity island 1 and 2 (spi-1 and -2) for the virulence of two non-host-specific, but in-vivo differently invasive, salmonella serovars in conjunction with the immune reaction of the host. primary avian splenic macrophages were inoculated with salmonella enterica sub-species enterica serovar (s.) typhimuri ...	2015	25811871
updated recommendations for the use of typhoid vaccine--advisory committee on immunization practices, united states, 2015.	these revised recommendations of the advisory committee on immunization practices update recommendations published in mmwr in 1994 and include updated information on the two currently available vaccines and on vaccine safety. they also include an update on the epidemiology of enteric fever in the united states, focusing on increasing drug resistance in salmonella enterica serotype typhi, the cause of typhoid fever, as well as the emergence of salmonella serotype paratyphi a, a cause of paratypho ...	2015	25811680
multidrug-resistant salmonella enterica serotype typhi, gulf of guinea region, africa.	we identified 3 lineages among multidrug-resistant (mdr) salmonella enterica serotype typhi isolates in the gulf of guinea region in africa during the 2000s. however, the mdr h58 haplotype, which predominates in southern asia and kenya, was not identified. mdr quinolone-susceptible isolates contained a 190-kb inchi1 pst2 plasmid or a 50-kb incn pst3 plasmid.	2015	25811307
the effective differentiation of salmonella isolates using four pcr-based typing methods.	discrimination of salmonella strains below the species level is very important to trace the source of outbreaks. to this end molecular typing methods can be successfully applied to routine analysis in nonspecialized laboratories due to their simplicity and speed. here, the discriminatory ability of four molecular typing methods was investigated in 74 salmonella enterica isolates.	2015	25809397
enteric fever and its impact on returning travellers.	enteric fever, a systemic illness, is caused by salmonella enterica serovar typhi or s. enterica serovar paratyphi a, b or c. the organism is transmitted to humans by the faecal oral route and is endemic in countries with poor sanitation and lacking clean drinking water. there are around 27 million individuals infected with s. typhi worldwide annually. enteric fever is a particular problem in travellers to endemic areas, especially those visiting friends and relatives. currently, the two main va ...	2015	25808163
activity of a bacterial cell envelope stress response is controlled by the interaction of a protein binding domain with different partners.	the bacterial phage shock protein (psp) system is a highly conserved cell envelope stress response required for virulence in yersinia enterocolitica and salmonella enterica. in non-inducing conditions the transcription factor pspf is inhibited by an interaction with pspa. in contrast, pspa associates with the cytoplasmic membrane proteins pspbc during inducing conditions. this has led to the proposal that pspbc exists in an off state, which cannot recruit pspa, or an on state, which can. however ...	2015	25802329
take the tube: remodelling of the endosomal system by intracellular salmonella enterica.	salmonella enterica is a facultative intracellular pathogen residing in a unique host cell-derived membrane compartment, termed salmonella-containing vacuole or scv. by the activity of effector proteins translocated by the spi2-endoced type iii secretion system (t3ss), the biogenesis of the scv is manipulated to generate a habitat permissive for intracellular proliferation. by taking control of the host cell vesicle fusion machinery, intracellular salmonella creates an extensive interconnected s ...	2015	25802001
the distribution of salmonella enterica serovars and subtypes in surface water from five agricultural regions across canada.	serovar prevalence of the zoonotic pathogen, salmonella enterica, was compared among 1624 surface water samples collected previously from five different canadian agricultural watersheds over multiple years. phagetyping, pulsed field gel electrophoresis (pfge), and antimicrobial resistance subtyping assays were performed on serovars enteritidis, typhimurium, and heidelberg. serovars and subtypes from surface water were compared with those from animal feces, human sewage, and serovars reported to ...	2015	25799976
reduction of salmonella enterica serotype poona and background microbiota on fresh-cut cantaloupe by electron beam irradiation.	the efficacy of electron beam (e-beam) irradiation processing to reduce salmonella enterica serotype poona on surfaces of fresh-cut cantaloupe, and the impact of e-beam irradiation processing on the numbers of indigenous microorganisms were determined. additionally, the d10-value for s. poona reduction on the cut cantaloupe was also determined. fresh-cut cantaloupe pieces, inoculated with s. poona to 7.8 log10 cfu/g, were exposed to 0.0, 0.7, or 1.5 kgy. surviving s. poona, lactic acid bacteria ...	2015	25796062
carbapenemase-producing salmonella enterica isolates in the uk.		2015	25795771
acquisition of iron is required for growth of salmonella spp. in tomato fruit.	salmonella remains a leading cause of bacterial food-borne disease, sickening millions each year. although outbreaks of salmonellosis have traditionally been associated with contaminated meat products, recent years have seen numerous disease cases caused by the consumption of produce. tomatoes have been specifically implicated, due to the ability of salmonella spp. to enter the tomato fruit and proliferate within, making the decontamination of the raw product impossible. to investigate the genet ...	2015	25795672
draft genome sequences of 33 salmonella enterica clinical and wildlife isolates from chile.	salmonella enterica causes health problem worldwide. the relationships among strains that are from the same serotype but different hosts, countries, and continents remain elusive. few genome sequences are available from s. enterica isolates from south america. therefore, we sequenced the genomes of 33 strains from diverse sources isolated in chile and determined that they were of different serotypes. these genomes will improve phylogenetic analysis of salmonella strains from chile and the rest o ...	2015	25792040
fate of mesophilic aerobic bacteria and salmonella enterica on the surface of eggs as affected by chicken feces, storage temperature, and relative humidity.	we compared the microbiological quality of chicken eggshells obtained from a traditional wholesale market and a modern supermarket. we also determined the survival and growth characteristics of naturally occurring mesophilic aerobic bacteria (mab) and artificially inoculated salmonella enterica on eggshells under various environmental conditions (presence of chicken feces, temperature [4, 12, or 25 °c], and relative humidity [rh; 43 or 85%]). the populations of mab, coliforms, and molds and yeas ...	2015	25791009
east london experience with enteric fever 2007-2012.	the clinical presentation and epidemiology for patients with enteric fever at two hospitals in east london during 2007-2012 is described with the aim to identify preventive opportunities and to reduce the cost of treatment.	2015	25790017
molecular ruler determines needle length for the salmonella spi-1 injectisome.	the type-iii secretion (t3s) systems of bacteria are part of self-assembling nanomachines: the bacterial flagellum that enables cells to propel themselves through liquid and across hydrated surfaces, and the injectisome that delivers pathogenic effector proteins into eukaryotic host cells. although the flagellum and injectisome serve different purposes, they are evolutionarily related and share many structural similarities. core features to these t3s systems are intrinsic length control mechanis ...	2015	25775540
antimicrobial resistance of non-typhoidal salmonella isolates from egg layer flocks and egg shells.	this study was conducted to examine the antimicrobial resistance (amr) of salmonella spp. isolated from commercial caged layer flocks in new south wales and south australia. all salmonella isolates (n=145) were subjected to phenotypic and genotypic characterisation of amr and carriage of integrons. the majority of salmonella isolates (91.72%) were susceptible to all antimicrobials tested in this study. limited resistance was observed to amoxicillin and ampicillin (5.51%), tetracycline (4.13%), c ...	2015	25770430
acquisition of extended-spectrum cephalosporin- and colistin-resistant salmonella enterica subsp. enterica serotype newport by pilgrims during hajj.	gatherings like the hajj involving many people who travel from different parts of the world represent a risk for the acquisition and dissemination of infectious diseases. in this study, acquisition of multidrug-resistant (mdr) salmonella spp. in 2013 hajj pilgrims from marseille, france, was investigated. in total, 267 rectal swabs were collected from 129 participants before their departure and after their return from the pilgrimage as well as during the pilgrimage from patients with diarrhoea. ...	2015	25769786
turtle-associated salmonella infections in kanagawa, japan.	in this paper, we examine 2 case reports for different reptile-related salmonella enterica subspecies enterica serotypes. in case 1, a 5-year-old boy presented with gastroenteritis caused by s. enterica subspecies enterica serovar poona. the suspected source of infection was a turtle kept at the patient's home. in case 2, a 4-year-old boy presented with gastroenteritis caused by s. enterica subspecies enterica serovar abony. the pulsed-field gel electrophoresis analysis suggested that a tortoise ...	2015	25766610
peritonitis as presentation of aorto-caval fistula with salmonella choleraesuis-associated abdominal aortic aneurysm.	the majority of aorto-caval fistulae occur spontaneously, either as a result of rupture of an existing atherosclerotic abdominal aortic aneurysm into the vena cava or secondary to iatrogenic injuries during peripheral angiography or surgery. aorto-caval fistula from an infected aortic aneurysm is a rare scenario, but potentially lethal.	2015	25761084
older leaves of lettuce (lactuca spp.) support higher levels of salmonella enterica ser. senftenberg attachment and show greater variation between plant accessions than do younger leaves.	salmonella can bind to the leaves of salad crops including lettuce and survive for commercially relevant periods. previous studies have shown that younger leaves are more susceptible to colonization than older leaves and that colonization levels are dependent on both the bacterial serovar and the lettuce cultivar. in this study, we investigated the ability of two lactuca sativa cultivars (saladin and iceberg) and an accession of wild lettuce (l. serriola) to support attachment of salmonella ente ...	2015	25953858
a handheld flow genetic analysis system (fgas): towards rapid, sensitive, quantitative and multiplex molecular diagnosis at the point-of-care level.	a handheld flow genetic analysis system (fgas) is proposed for rapid, sensitive, multiplex and real-time quantification of nucleic acids at the point-of-care (poc) level. the fgas includes a helical thermal-gradient microreactor and a microflow actuator, as well as control circuitry for temperature, fluid and power management, and smartphone fluorescence imaging. all of these features are integrated into a field-portable and easy-to-use molecular diagnostic platform powered by lithium batteries. ...	2015	25953325
salmonella in raw chicken meat from the romanian seaside: frequency of isolation and antibiotic resistance.	the purpose of this study was to investigate the occurrence of salmonella in raw chicken meat samples collected at the romanian seaside and to evaluate the antimicrobial susceptibility of the isolates. in 2012, 317 chicken meat samples from slaughterhouses (n = 289) and retail markets (n = 28) were evaluated. overall, 13.2% (42) of the samples contained salmonella; 12.8% (37) and 17.8% (5) from the chicken carcasses at slaughterhouses and the fresh meat from retail markets, respectively. eight s ...	2015	25951397
multiplex pcr-based serogrouping and serotyping of salmonella enterica from tonsil and jejunum with jejunal lymph nodes of slaughtered swine in metro manila, philippines.	food poisoning outbreaks and livestock mortalities caused by salmonella enterica are widespread in the philippines, with hogs being the most commonly recognized carriers of the pathogen. to prevent and control the occurrence of s. enterica infection in the country, methods were used in this study to isolate and rapidly detect, differentiate, and characterize s. enterica in tonsils and jejuna with jejunal lymph nodes of swine slaughtered in four locally registered meat establishments (lrmes) and ...	2015	25951380
development of combined dry heat and chlorine dioxide gas treatment with mechanical mixing for inactivation of salmonella enterica serovar montevideo on mung bean seeds.	foodborne outbreaks have been associated with the consumption of fresh sprouted beans. the sprouting conditions of mung bean seeds provide optimal conditions of temperature and relative humidity for any potential pathogenic contaminant on the seeds to grow. the lack of a kill step postsprouting is a major safety concern. thus, the use of a kill step on the seeds prior to a sprouting step would enhance the safety of fresh sprouts. the objective of this work was to evaluate the effectiveness of th ...	2015	25951379