Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| [influenza virus in pediatrics. a reason for hospitalization]. | influenza infection in infants and children has been classically underestimated due to its non-specific symptoms, which sometimes overlap those of other respiratory viruses. infants under 24 months are a risk group and school-aged children are a major source of influenza infection. the aim of this study was to describe the clinical and epidemiological characteristics of children hospitalized for flu, including co-infections and the differences as compared to other respiratory viruses. the effect ... | 2007 | 17335696 |
| age-related difference in immune responses to respiratory syncytial virus infection in young children. | there have been longitudinal studies of the developmental change of the immune system during the first year of life. the aim of this study was to investigate if there is any age-related difference in cytokine responses to respiratory syncytial virus (rsv) infection between the patients under 6 months of age and the patients over 12 months of age compared with age-matched controls. forty-five children < or =24 months of age who were admitted with acute rsv bronchiolitis were enrolled. the patient ... | 2007 | 17338780 |
| nasal lavage leukotrienes in infants with rsv bronchiolitis. | respiratory syncytial virus (rsv) bronchiolitis is a very common infection in infants and, after the acute phase, a number of patients develop a reactive airway disease that lasts for years. although the pathogenesis of the lung damage after rsv bronchiolitis is still largely unknown, previous studies suggest that leukotrienes may play an active part in it. the aim of this study was to measure leukotriene levels in the nasal lavage fluid (nlf) collected in infants during rsv bronchiolitis and 1 ... | 2007 | 17338781 |
| consumption of asthma medication after rs-virus epidemic--a population based survey. | it has been suggested that a respiratory syncytial virus (rsv) infection in infancy increases the likelihood of development of asthma in childhood. the rsv epidemics have a special 2-yr pattern in finland and this allows the evaluation of the association of rsv and asthma by epidemiological means. we evaluated whether being 0-6 months of age during an rsv epidemic has an impact on the use of asthma medication later in the childhood. the consumption of asthma medication at the age of 3-16 yr and ... | 2007 | 17338782 |
| 1,4-benzodiazepines as inhibitors of respiratory syncytial virus. the identification of a clinical candidate. | respiratory syncytial virus (rsv) is the cause of one-fifth of all lower respiratory tract infections worldwide and is increasingly being recognized as representing a serious threat to patient groups with poorly functioning or immature immune systems. racemic 1,4-benzodiazepines show potent anti-rsv activity in vitro. anti-rsv evaluation of 3-position r- and s-benzodiazepine enantiomers and subsequent optimization of this series resulted in selection of a clinical candidate. antiviral activity w ... | 2007 | 17341059 |
| the relationship of meteorological conditions to the epidemic activity of respiratory syncytial virus. | our aim was to obtain knowledge of how meteorological conditions affect community epidemics of respiratory syncytial virus (rsv) infection. to this end we recorded year-round rsv activity in nine cities that differ markedly in geographic location and climate. we correlated local weather conditions with weekly or monthly rsv cases. we reviewed similar reports from other areas varying in climate. weekly rsv activity was related to temperature in a bimodal fashion, with peaks of activity at tempera ... | 2007 | 17346359 |
| rnai-dependent and -independent antiviral phenotypes of chromosomally integrated shrna clones: role of vasp in respiratory syncytial virus growth. | stable rna interference (rnai) is commonly achieved by recombinant expression of short hairpin rna (shrna). to generate virus-resistant cell lines, we cloned a shrna cassette against the phosphoprotein gene of respiratory syncytial virus (rsv) into a poliii-driven plasmid vector. analysis of individual stable transfectants showed a spectrum of rsv resistance correlating with the levels of shrna expressed from different chromosomal locations. interestingly, resistance in a minority of clones was ... | 2007 | 17351763 |
| recombinant human deoxyribonuclease in infants with respiratory syncytial virus bronchiolitis. | treatment of hospitalized infants with respiratory syncytial virus (rsv) bronchiolitis is mainly supportive. bronchodilators and systemic steroids are often used but do not reduce the length of hospital stay. because hypoxia and airways obstruction develop secondary to viscous mucus in infants with rsv bronchiolitis, and because free dna is present in rsv mucus, we tested the efficacy of the mucolytic drug recombinant human deoxyribonuclease (rhdnase). | 2007 | 17356094 |
| severe human lower respiratory tract illness caused by respiratory syncytial virus and influenza virus is characterized by the absence of pulmonary cytotoxic lymphocyte responses. | respiratory syncytial virus (rsv) and influenza virus are common causes of infantile lower respiratory tract infection (lrti). it is widely believed that both viral replication and inappropriately enhanced immune responses contribute to disease severity. in infants, rsv lrti is known to be more severe than influenza virus lrti. | 2007 | 17357048 |
| antiviral triterpenoids from the medicinal plant schefflera heptaphylla. | schefflera heptaphylla (l.) frodin is a principal ingredient of an herbal tea formulation widely used for the treatment of common cold in southern china. an extract of the long leafstalk of the compound leaf of s. heptaphylla exhibited the most potent antiviral activity against respiratory syncytial virus (rsv). further antiviral-guided fractionation and isolation of the leafstalk extract of s. heptaphylla led to obtain two highly active pure triterpenoids, namely 3alpha-hydroxylup-20(29)-ene-23 ... | 2007 | 17357972 |
| development of motavizumab, an ultra-potent antibody for the prevention of respiratory syncytial virus infection in the upper and lower respiratory tract. | respiratory syncytial virus (rsv) is the leading cause of viral bronchiolitis and pneumonia in infants and children. currently, palivizumab is the only approved monoclonal antibody (mab) for prophylaxis of rsv. however, a small percentage of patients are not protected by palivizumab; in addition, palivizumab does not inhibit rsv replication effectively in the upper respiratory tract. we report here the development and characterization of motavizumab, an ultra-potent, affinity-matured, humanized ... | 2007 | 17362988 |
| respiratory syncytial virus infection in anesthetized weanling rather than adult rats prolongs the apneic responses to right atrial injection of capsaicin. | apnea is a common complication in infants infected by respiratory syncytial virus (rsv). a recent study has shown that intranasal inoculation of rsv in conscious weanling rats strengthens the apneic responses to right atrial injection of capsaicin (cap), leading to 66% mortality. the objectives of the present study were to determine 1) whether rsv infection changes baseline minute ventilation (ve) and arterial blood gases in anesthetized rats; 2) what the effects of rsv infection are on the resp ... | 2007 | 17363622 |
| c57bl/6 mice are protected from respiratory syncytial virus (rsv) challenge and il-5 associated pulmonary eosinophilic infiltrates following intranasal immunization with protollin-ersv vaccine. | the protective efficacy of an intranasal (in) protollin-ersv vaccine has recently been demonstrated in the rsv-susceptible balb/c mouse model. here, we report the safety, immunogenicity and efficacy of protollin-ersv vaccine in the relatively resistant c57bl/6 mouse model. c57bl/6 mice immunized in with either two or three doses of protollin-ersv produced significant systemic and mucosal rsv-specific antibodies. mice immunized with the protollin vaccine displayed polarized th1 responses with aug ... | 2007 | 17374422 |
| mechanistic analysis and comparison of viral fusogenic membrane proteins for their synergistic effects on chemotherapy. | previously we demonstrated that the expression of fusogenic membrane proteins (fmg) of measles virus (mv-h/f) can synergistically enhance chemotherapy. in this study, we used median-effect analysis to evaluate whether the expression of respiratory syncytial virus (rsv-f), as well as vesicular stomatitis virus (vsv-g) can also synergistically enhance chemotherapy. furthermore we elucidated by western blot analysis some molecular pathways that might be responsible for this effect. we showed in col ... | 2007 | 17374986 |
| host transcription profiles upon primary respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is a common cause of severe lower respiratory tract infection in children. severe rsv disease is related to an inappropriate immune response to rsv resulting in enhanced lung pathology which is influenced by host genetic factors. to gain insight into the early pathways of the pathogenesis of and immune response to rsv infection, we determined the transcription profiles of lungs and lymph nodes on days 1 and 3 after infection of mice. primary rsv infection result ... | 2007 | 17376894 |
| bronchiolitis due to respiratory syncytial virus in hospitalized children: a study of seasonal rhythm. | the objective of this study was to describe the rhythm of respiratory syncytial virus (rsv) bronchiolitis seasonal outbreaks in hospitalized children. | 2007 | 17381468 |
| human bocavirus in iranian children with acute respiratory infections. | human bocavirus (hbov), a virus discovered in sweden in 2005, has been associated with acute respiratory infections in young children and subsequent reports suggest that hbov may have a worldwide distribution. this report describes the frequency and clinical presentation of hbov in 261 iranian children<5 years old with acute respiratory infections attending two regional hospitals in rasht, iran in the winter of 2003-2004. polymerase chain reaction (pcr) and reverse transcription pcr (rt-pcr) wer ... | 2007 | 17385723 |
| rsv-infected airway epithelial cells cause biphasic up-regulation of ccr1 expression on human monocytes. | respiratory syncytial virus (rsv) infection can cause extensive airway inflammation, which is orchestrated by chemokines and their receptors. rsv-infected epithelial cells secrete many cytokines and chemokines, but little is known about regulation of chemokine receptors on target cells. we investigated the effects of conditioned media (cm) from rsv-infected epithelial cells on monocyte ccr1, ccr2, and ccr5 expression. rsv-cm but not control-cm stimulated a biphasic increase in cell-surface ccr1, ... | 2007 | 17389578 |
| molecular investigations of an outbreak of parainfluenza virus type 3 and respiratory syncytial virus infections in a hematology unit. | a large simultaneous outbreak of respiratory syncytial virus (rsv) and parainfluenza type 3 (piv-3) infections occurred on an adult hematology unit. implementation of enhanced infection control was complicated by cocirculation of the two different viruses, with prolonged viral shedding from infected patients, and placed great pressure on health care staff; of 27 infected hematopoietic stem cell transplant patients, 9 died, and the unit was closed for 2 months. retrospective molecular investigati ... | 2007 | 17392447 |
| immunization strategies for the prevention of pneumovirus infections. | pneumoviruses, which are viruses of the family paramyxoviridae, subfamily pneumovirinae, are pathogens that infect the respiratory tract of their host species. the human pneumovirus pathogen, human respiratory syncytial virus (rsv), has counterparts that infect cows (bovine rsv), sheep (ovine rsv), goats (caprine rsv) and rodents (pneumonia virus of mice). each pneumovirus is host specific and results in a spectrum of disease, ranging from mild upper-respiratory illness to severe bronchiolitis a ... | 2007 | 17408367 |
| t helper 1 background protects against airway hyperresponsiveness and inflammation in guinea pigs with persistent respiratory syncytial virus infection. | a family history of allergy has been implicated in children who develop post-bronchiolitis wheezing and asthma. in a guinea pig model of respiratory syncytial virus (rsv) lung infection, we evaluated the role of host th1 background (either genetic or induced) on the development of a persistent infection, nonspecific airway hyperresponsiveness (ahr) and airway inflammation. allergy resistant/t helper 1 (th1)-skewed strain 2 guinea pigs (str2) and cytosine phosphate guanine oligodeoxynucleotides ( ... | 2007 | 17413871 |
| lack of usefulness of an abnormal white blood cell count for predicting a concurrent serious bacterial infection in infants and young children hospitalized with respiratory syncytial virus lower respiratory tract infection. | there continues to be controversy on the most appropriate way to manage infants and young children with fever and documented rsv lower respiratory tract infection (lrti). the objective of this study was to determine the usefulness of an abnormal white blood cell (wbc) count for predicting a concurrent serious bacterial infection in patients admitted with rsv lrti. | 2007 | 17414393 |
| pertussis is common in nonvaccinated infants hospitalized for respiratory syncytial virus infection. | there is some evidence that bordetella pertussis can cause co-infection with respiratory syncytial virus (rsv). | 2007 | 17414394 |
| palivizumab prophylaxis to prevent respiratory syncytial virus mortality after pediatric bone marrow transplantation: a decision analysis model. | palivizumab, a monoclonal antibody against respiratory syncytial virus (rsv), has been demonstrated to be safe and effective in young children, but evidence is lacking as to whether palivizumab is effective in preventing rsv-induced morbidity and mortality in children who are immunosuppressed after bone marrow transplantation (bmt). as a randomized, double-blind, placebo-controlled trial is lacking, we chose to examine this issue with the use of decision analysis methodology. | 2007 | 17414564 |
| intranasal vaccination with peptides and cholera toxin subunit b as adjuvant to enhance mucosal and systemic immunity to respiratory syncytial virus. | amino acid sequences 200-225 and 255-278 of the f protein of human respiratory syncytial virus (hrsv) are t cell epitopes (bourgeois et al., 1991; corvaisier et al, 1993). peptides corresponding to these two regions were synthesized and coupled with keyhole limpet haemocyanin (klh). the two conjugated proteins were administered intranasally to balb/c mice alone or together with cholera toxin b (ctb). elisas revealed that the mixture of the conjugates with ctb increased not only the systemic resp ... | 2007 | 17424945 |
| neonatal ovine pulmonary dendritic cells support bovine respiratory syncytial virus replication with enhanced interleukin (il)-4 and il-10 gene transcripts. | the lung microenvironment is constantly exposed to microorganisms and particulate matter. lung dendritic cells (dcs) play a crucial role in the uptake and processing of antigens found within the respiratory tract. respiratory syncytial virus (rsv) is a common respiratory tract pathogen in children that induces an influx of dcs to the mucosal surfaces of the lung. using a neonatal lamb model, we examined the in vivo permissiveness of dcs to rsv infection, as well as overall cell surface changes a ... | 2007 | 17425426 |
| pretreatment with recombinant human vascular endothelial growth factor reduces virus replication and inflammation in a perinatal lamb model of respiratory syncytial virus infection. | vascular endothelial growth factor (vegf) is increasingly recognized as a perinatal regulator of lung maturation and surfactant protein expression. preterm and young infants are at increased risk for pulmonary immaturity characterized by insufficient surfactant production as well as increased risk for severe manifestations of respiratory syncytial virus (rsv) infection. innate immune components including surfactant proteins a and d, and beta-defensins have putative antimicrobial activity against ... | 2007 | 17425433 |
| peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells. | respiratory syncytial virus (rsv) is the major causative agent of severe lower respiratory tract disease and death in infants worldwide. the epithelial cells of the airways are the target cells for rsv infection and the site of the majority of the inflammation associated with the disease. however, despite five decades of intensive rsv research there exist neither an effective active vaccine nor a promising antiviral and anti-inflammatory therapy. recently, peroxisome proliferator-activated recep ... | 2007 | 17425601 |
| high-throughput screening of stabilizers for respiratory syncytial virus: identification of stabilizers and their effects on the conformational thermostability of viral particles. | respiratory syncytial virus (rsv) is the leading cause of severe respiratory infection in children worldwide. recombinant live attenuated viral preparations are one of the most promising strategies for vaccination but they typically possess poor thermostability. in this work, a library of compounds was screened and stabilizers were selected based on their ability to inhibit the aggregation of rsv perturbed at 56 degrees c. after screening and selection of excipients, the conformational stability ... | 2007 | 17426457 |
| human metapneumovirus bronchiolitis in infancy is an important risk factor for asthma at age 5. | human metapneumovirus (hmpv) infection plays an important role in the pediatric respiratory infections. however, little is known about the relationship between hmpv-bronchiolitis and the development of subsequent wheezing. the aim of this study was to evaluate the outcome at third to fifth year after admission for hmpv-bronchiolitis and to compare it with children admitted for respiratory syncytial virus (rsv)-bronchiolitis and with children without lower respiratory disease in the first 2 years ... | 2007 | 17427899 |
| performance of a rapid assay (binax now) for detection of respiratory syncytial virus at a children's hospital over a 3-year period. | a rapid assay, binax now rsv, was compared to viral culture for 14,756 pediatric respiratory specimens obtained from 2003 to 2006. there were 794 viral culture-confirmed respiratory syncytial virus infections. sensitivity was 81%, and specificity was 93.2%. sensitivity was greatest for neonates (91.1% versus 80.7% [p < 0.01]). | 2007 | 17428942 |
| phenotype of acute respiratory syncytial virus induced lower respiratory tract illness in infancy and subsequent morbidity. | the objective of this study was to investigate the association hypothesis that outcome following respiratory syncytial virus (rsv) induced bronchiolitis (rsvb) and rsv induced wheeze (rsvw) are different. at 3 years respiratory symptoms were more common in those with rsv infection than the control group but there was no increase in allergic sensitisation (11% vs 10%). those with rsvw were more likely to have evidence of allergic sensitisation when compared with rsvb subjects (22% vs 7%), and hav ... | 2007 | 17429926 |
| high-efficiency promoter-dependent transduction by adeno-associated virus type 6 vectors in mouse lung. | the transduction efficiency of adeno-associated virus (aav) vectors in various somatic tissues has been shown to depend heavily on the aav type from which the vector capsid proteins are derived. among the aav types studied, aav6 efficiently transduces cells of the airway epithelium, making it a good candidate for the treatment of lung diseases such as cystic fibrosis. here we have evaluated the effects of various promoter sequences on transduction rates and gene expression levels in the lung. of ... | 2007 | 17430088 |
| respiratory syncytial virus induces insensitivity to beta-adrenergic agonists in mouse lung epithelium in vivo. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis in infants and children worldwide. we wished to determine whether intratracheal administration of beta-agonists improved alveolar fluid clearance (afc) across the distal respiratory epithelium of rsv-infected mice. following intranasal infection with rsv strain a2, afc was measured in anesthetized, ventilated balb/c mice by instillation of 5% bsa into the dependent lung. we found that direct activation of protein kinase ... | 2007 | 17435077 |
| evaluation of uplink-rsv: prototype rapid antigen test for detection of respiratory syncytial virus infection. | a prototype rapid antigen test for the on-site detection of respiratory syncytial virus (rsv) infection was developed and evaluated. the platform uses instrumented assay analysis, eliminating potential operator bias in the interpretation of the test result that may occur with visually interpreted rapid antigen assays. the device was tested as the first point-of-care (poc) infectious disease application of novel reporter up-converting phosphor technology (upt) using a specifically designed portab ... | 2007 | 17435154 |
| immunopathogenesis of respiratory syncytial virus bronchiolitis. | the objective of this study was to elucidate the relation between respiratory syncytial virus (rsv) infection and cytokine/chemokine concentrations, as well as the impact that these factors have on the severity of bronchiolitis. | 2007 | 17436234 |
| myd88-mediated instructive signals in dendritic cells regulate pulmonary immune responses during respiratory virus infection. | respiratory syncytial virus (rsv) is the leading cause of respiratory disease in infants worldwide. the induction of innate immunity and the establishment of adaptive immune responses are influenced by the recognition of pathogen-associated molecular patterns by tlrs. one of the primary pathways for tlr activation is by myd88 adapter protein signaling. the present studies indicate that myd88 deficiency profoundly impacts the pulmonary environment in rsv-infected mice characterized by the accumul ... | 2007 | 17442966 |
| adult asthma after non-respiratory syncytial virus bronchiolitis in infancy: subgroup analysis of the 20-year prospective follow-up study. | recent studies have stressed the influence of other viruses than respiratory syncytial virus (rsv) in the development of asthma in later childhood after bronchiolitis in infancy. however, the virus-specific prognosis until adulthood has remained obscure, due to lack of sufficiently long follow-up studies. the aim of the present study was to evaluate adult respiratory morbidity after bronchiolitis in infancy, focused on cases not caused by rsv. | 2007 | 17445037 |
| transcriptional profile of rous sarcoma virus transformed chicken embryo fibroblasts reveals new signaling targets of viral-src. | transformation of chicken fibroblasts in vitro by rous sarcoma virus represents a model of cancer in which a single oncogene, viral src, uniformly and rapidly transforms primary cells in culture. we experimentally surveyed the transcriptional program affected by rous sarcoma virus (rsv) in primary culture of chicken embryo fibroblasts. as a control, we used cells infected with non-transforming rsv mutant td106, in which the src gene was deleted. using affymetrix genechip chicken genome arrays, w ... | 2007 | 17448514 |
| common human toll-like receptor 4 polymorphisms--role in susceptibility to respiratory syncytial virus infection and functional immunological relevance. | evidence suggests that toll-like receptor 4 (tlr4) contributes to immune recognition of respiratory syncytial virus (rsv). the tlr4 gene harbours a polymorphism-asp299gly-previously associated with reduced tlr4 signalling. to understand of how host genetic variation influences the outcome of rsv infection in children, we examined the association between the tlr4 299gly allele and severe rsv disease. by genotyping 236 children with rsv infection and 219 healthy controls we found no association be ... | 2007 | 17449325 |
| [association of interleukin 8 single nucleotide polymorphisms with the susceptibility to respiratory syncytial virus infection]. | to explore the association of interleukin 8 (il-8)-251t/a and 781 c/t single nucleotide polymorphisms (snps) with the susceptibility of infants and young children to respiratory syncytial virus (rsv) infection. | 2007 | 17456336 |
| genetic variability of the fusion protein and circulation patterns of genotypes of the respiratory syncytial virus. | although antigenic and genetic variations were shown to occur both in the g and f protein of respiratory syncytial virus (rsv), few studies looked at the variation of f gene. the f genotypes were determined by the evaluation of clustering patterns, via the phylogenetic analysis of the nucleotide sequences of a variable region in the f gene. one hundred seventy-nine strains obtained from a children's hospital in korea over nine consecutive epidemics were included. the relationship between the f a ... | 2007 | 17457915 |
| respiratory syncytial virus infection in adults. | respiratory syncytial virus (rsv) is a common winter time respiratory virus that affects persons of all ages and is the major cause of serious lower respiratory tract infections in young children. however, rsv is also an important pathogen in adults, particularly in the elderly, patients with chronic lung disease, or those with impaired immunity. clinical features of rsv infections overlap with other respiratory viruses, so laboratory tests are required to establish the diagnosis. reverse transc ... | 2007 | 17458771 |
| [simultaneous outbreaks of rotavirus and respiratory syncytial virus in paris: a 12-year survey]. | the authors had for aim to study the coincidence of rsv and rotavirus epidemic peaks in pediatric patients hospitalized in the paris area. | 2007 | 17459636 |
| development of a 1-step enzyme-linked immunosorbent assay for the rapid diagnosis of bovine respiratory syncytial virus in postmortem specimens. | bovine respiratory syncytial virus (brsv) is associated with severe respiratory disease in cattle. brsv infection frequently leads to the death of young infected animals. the presence of brsv in postmortem specimens is routinely detected using indirect immunofluorescence (iif). however, this technique requires special equipment and considerable expertise. the present paper describes the development of a 1-step elisa for rapid (1.5 hours) detection of brsv antigen in organ homogenates. the perfor ... | 2007 | 17459851 |
| dna immunization with plasmids encoding fusion and nucleocapsid proteins of bovine respiratory syncytial virus induces a strong cell-mediated immunity and protects calves against challenge. | respiratory syncytial viruses (rsv) are one of the most important respiratory pathogens of humans and cattle, and there is currently no safe and effective vaccine prophylaxis. in this study, we designed two codon-optimized plasmids encoding the bovine rsv fusion (f) and nucleocapsid (n) proteins and assessed their immunogenicity in young calves. two administrations of both plasmids elicited low antibody levels but primed a strong cell-mediated immunity characterized by lymphoproliferative respon ... | 2007 | 17459933 |
| il-10 and rantes are elevated in nasopharyngeal secretions of children with respiratory syncytial virus infection. | respiratory syncytial virus (rsv) infection causes asthma-like symptoms in infants and young children. although an increase in several mediators in the airway during rsv infection has been reported, the mechanisms involved in airway inflammation are not fully understood. the aim of this study was to investigate the immunological deviation associated with airway inflammation by measuring cytokine and chemokine levels in the airway during rsv infection. | 2007 | 17460443 |
| effects of respiratory syncytial virus infection on dendritic cells and cysteinyl leukotrienes in lung tissues of a murine model of asthma. | pulmonary dendritic cells (dcs) play critical roles in both allergy and in viral infection. levels of cysteinyl leukotrienes (cyslts) increase after allergen sensitization and viral infection and can modulate the migration and functions of dcs. the present study examines the effects of respiratory syncytial virus (rsv) infection on numbers of dcs and cyslt concentrations in lung tissues of mice sensitized with mite allergen. | 2007 | 17460444 |
| promoter effects of adeno-associated viral vector for transgene expression in the cochlea in vivo. | the aims of this study were to evaluate the expression of enhanced green fluorescent protein (egfp) driven by 6 different promoters, including cytomegalovirus ie enhancer and chicken beta-actin promoter (cag), cytomegalovirus promoter (cmv), neuron-specific enolase promoter (nse), myosin 7a promoter (myo), elongation factor 1alpha promoter (ef-1alpha), and rous sarcoma virus promoter (rsv), and assess the dose response of cag promoter to transgene expression in the cochlea. serotype 1 adeno-asso ... | 2007 | 17464178 |
| cd14 -550 c/t, which is related to the serum level of soluble cd14, is associated with the development of respiratory syncytial virus bronchiolitis in the japanese population. | the contribution that genetic polymorphisms of toll-like receptor (tlr) 4 and of cd14--both of which recognize respiratory syncytial virus (rsv) in the innate immune response--make to rsv bronchiolitis in the japanese population has not yet been clarified. | 2007 | 17471431 |
| characterization of cd4+ memory t cell responses directed against common respiratory pathogens in peripheral blood and lung. | we investigated cd4(+) memory t cell responses to influenza virus (flu), respiratory syncytial virus (rsv), and nontypeable haemophilus influenzae (nthi). | 2007 | 17471443 |
| respiratory syncytial virus differentially activates murine myeloid and plasmacytoid dendritic cells. | respiratory syncytial virus (rsv) is the primary cause of bronchiolitis in young children. upon infection both t helper 1 (th1) and th2 cytokines are produced. because rsv-induced th2 responses have been associated with severe immunopathology and aggravation of allergic reactions, the regulation of the immune response following rsv infection is crucial. in this study we examined the influence of rsv on the activation and function of murine bone marrow-derived dendritic cells (dcs). rsv induced t ... | 2007 | 17472722 |
| respiratory syncytial virus (rsv) in infants hospitalized for acute lower respiratory tract disease: incidence and associated risks. | respiratory syncytial virus (rsv) is one of the main causes of acute lower respiratory tract infections worldwide. we examined the incidence and associated risks for rsv infection in infants hospitalized in two university hospitals in the state of são paulo. we made a prospective cohort study involving 152 infants hospitalized for acute lower respiratory tract infections (alrti) in two university hospitals in campinas, são paulo, brazil, between april and september 2004. clinical and epidemiolog ... | 2006 | 17293926 |
| performance testing of two new one-step real time pcr assays for detection of human influenza and avian influenza viruses isolated in humans and respiratory syncytial virus. | two real time one-step rt-pcr assays were developed for simultaneous detection and typing of influenza a and b viruses and detection of respiratory syncytial virus (rsv). as regard influenza, primers were designed to amplify specific sequences of gene m of a/h1n1, a/h3n2, a/h5n1, a/h7n7 and a/h9n2 viruses and of gene np of type b viruses belonging both yamagata and victoria lineage. specificity, analytical and clinical sensitivity, dynamic range, linearity of the new assays were evaluated. | 2006 | 17263157 |
| tlr-4 and cd14 polymorphisms in respiratory syncytial virus associated disease. | respiratory syncytial virus (rsv) is the most common viral respiratory pathogen during infancy world wide. it induces innate and adaptive immune response in host cells. the toll like receptor 4 (tlr4)/cd14 complex is particularly important for the initiation of an innate immune response to rsv. thus we were interested whether an association exists between severe rsv associated diseases and polymorphisms within tlr4 and cd14. we genotyped the cd14 promotor polymorphism -c159t and the two common t ... | 2006 | 17264400 |
| whole blood gene expression in infants with respiratory syncytial virus bronchiolitis. | respiratory syncytial virus (rsv) is a major cause of viral bronchiolitis in infants worldwide, and environmental, viral and host factors are all of importance for disease susceptibility and severity. to study the systemic host response to this disease we used the microarray technology to measure mrna gene expression levels in whole blood of five male infants hospitalised with acute rsv, subtype b, bronchiolitis versus five one year old male controls exposed to rsv during infancy without bronchi ... | 2006 | 17166282 |
| [reproduction of severe asthma model in mice]. | to reproduce a severe asthma model in ovalbumin (ova)-sensitized balb/c mice by induction with respiratory syncytial virus (rsv). | 2006 | 17166354 |
| [surveillance for respiratory syncytial virus subtypes a and b in children with acute respiratory infections in beijing during 2000 to 2006 seasons]. | to characterize the prevalence and occurrence of subgroups of human respiratory syncytial virus (rsv) in infants and young children with acute respiratory infections (ari) in beijing area. | 2006 | 17254462 |
| t cell responses to respiratory syncytial virus fusion and attachment proteins in human peripheral blood mononuclear cells. | the cellular immune response to respiratory syncytial virus (rsv) is considered important in both protection and immunopathogenesis. we have studied the hla class i- and class ii-restricted t cell responses to rsv fusion (f) and attachment (g) proteins in peripheral blood mononuclear cells (pbmcs) obtained from healthy young adults. pbmcs were stimulated with autologous cells infected with recombinant modified vaccinia virus ankara (rmva) expressing rsv f (rmva-f) or g (rmva-g). in rmva-f-stimul ... | 2006 | 17201662 |
| [human metapneumovirus (hmpv) associated to severe bronchial asthmatic crisis]. | human metapneumovirus (hmpv) is a recently reported agent of acute infection in the respiratory tract. it has been found in children as well as in young adults and elders. the clinical manifestations produced by hmpv are indistinguishable from those by common respiratory virus, and can evolve from asymptomatic infection into severe pneumonia. on the other hand, some authors have described cases of bronchial asthma exacerbation associated with hmpv infection. in this work we report a case of a ch ... | 2006 | 17152212 |
| respiratory syncytial virus bronchiolitis : current and future strategies for treatment and prophylaxis. | respiratory syncytial virus (rsv) is the most important cause of viral lower respiratory tract illness in infants and children worldwide and is responsible for over 120 000 annual hospitalizations in infants in the us alone. rsv is also recognized as a major respiratory viral pathogen in the elderly and other high-risk populations. bronchiolitis, pneumonia, apnea, respiratory failure, and death are well known manifestations of severe acute rsv disease. rsv infection has also been associated with ... | 2006 | 17154675 |
| il-13 is associated with reduced illness and replication in primary respiratory syncytial virus infection in the mouse. | the role of il-13 in respiratory syncytial virus (rsv) immunopathogenesis is incompletely described. to assess the effect of il-13 on primary rsv infection, transgenic mice which either overexpress il-13 in the lung (il-13 oe) or non-transgenic littermates (il-13 nt) were challenged intranasally with rsv. il-13 oe mice had significantly decreased peak viral titers four days after infection compared to non-transgenic littermates. in addition, il-13 oe mice had significantly lower rsv-induced weig ... | 2006 | 17110149 |
| the effect of an interventional program on adherence to the american academy of pediatrics guidelines for palivizumab prophylaxis. | to determine the effect of an interventional program designed to improve adherence to american academy of pediatrics (aap) guidelines for palivizumab prophylaxis. | 2006 | 17072124 |
| long-term consequences of respiratory syncytial virus acute lower respiratory tract infection in early childhood in guinea-bissau. | the study aimed to investigate long-term consequences of respiratory syncytial virus (rsv) positive acute lower respiratory tract infection (alri) in a low-income country according to severity of the initial infection. | 2006 | 17072125 |
| viral infections in patients with hematological malignancies. | viral infections are important causes of morbidity and mortality for patients with a hematological malignancy. however, the true incidence and consequences of viral infections for these patients who undergo conventional nontransplant therapy are poorly defined. the difference in incidence and outcome of viral infections among patient groups is wide, but dependent upon the intensity and duration of t-cell-mediated immune suppression. infections caused by cytomegalovirus (cmv), herpes simplex viru ... | 2006 | 17124085 |
| [etiology of acute viral respiratory tract infections in children from gran canaria, the canary islands (spain)]. | acute respiratory tract infections (arti) of viral origin are a frequent cause of pediatric consultations and hospital admissions. the aim of this study was to investigate the etiology of these infections in gran canaria, the canary islands, (spain). | 2006 | 17125674 |
| decline in respiratory syncytial virus hospitalizations in a region with high hospitalization rates and prolonged season. | during 1993 to 1996, alaska native infants <1 year of age from the yukon kuskokwim (yk) delta in alaska experienced a respiratory syncytial virus (rsv) hospitalization rate 5 times the u.s. general infant population rate. we describe trends in lower respiratory tract infection (lrti) and rsv hospitalizations in yk children from 1994 to 2004. | 2006 | 17133156 |
| the risk of respiratory syncytial virus-related hospitalizations in preterm infants of 29 to 35 weeks' gestational age. | among 1158 preterm infants of 29-35 weeks' gestational age, respiratory syncytial virus (rsv) -related hospitalizations (rsv-h) occurred in 4.2% during the first year of life. four independent factors influenced the risk for rsv-h: neurologic problems (odds ratio [or], 3.6), male gender (or, 2.8), presence of an older sibling (or, 1.7) and discharge from october to december (or, 1.7). the estimated risk of rsv-h varied between 1% (no risk factor present) and 30% (4 risk factors present). | 2006 | 17133170 |
| brief report: respiratory syncytial virus activity--united states, 2005-2006. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infections (lrtis) (e.g., bronchiolitis and pneumonia) among young children in the united states. rsv also causes severe respiratory disease and a substantial number of deaths among older adults and persons with compromised respiratory, cardiac, or immune systems. rsv is transmitted person to person through close contact or inhalation of large droplets from a sneeze or cough; infection also can occur through contact wi ... | 2006 | 17136023 |
| surfactant protein polymorphisms and neonatal lung disease. | here, we describe the approach of defining the genetic contribution to disease and discuss the polymorphisms of some genes that are associated with respiratory disease. the common allelic variants of sp-a1, sp-a2, sp-b, sp-c, and sp-d genes are associated with respiratory distress syndrome (rds), bronchopulmonary dysplasia (bpd), or respiratory syncytial virus (rsv) bronchiolitis. the main sp-a haplotype, interactively with sp-b ile131thr polymorphism and with constitutional and environmental fa ... | 2006 | 17142161 |
| nak-associated protein 1 participates in both the tlr3 and the cytoplasmic pathways in type i ifn induction. | tlr3 and the cytoplasmic helicase family proteins (retinoic acid-inducible gene i (rig-i) and melanoma differentiation-associated gene 5 (mda5)) serve as dsrna pattern-recognition receptors. in response to poly(i:c), a representative of dsrna, and viral infection, they have been shown to activate the transcription factor ifn regulatory factor (irf)-3, which in turn induces activation of the ifn-beta promoter. rig-i/mda5 recognizes dsrna in the cytoplasm, whereas tlr3 resides in the cell surface ... | 2006 | 17142768 |
| [therapeutic monoclonal antibodics]. | less than ten years after the discovery of hybridomas by kohler and milstein in 1975, the first monoclonal antibody was injected in humans to prevent graft rejection. the antibodies used were targeted against the cd3 molecule involved in the transduction of t cell signals after recognition of the antigen. paradoxically, because of pharmaceutical hesitations later found to be unwarranted, the therapeutic class developed slowly. it was not until the 1990s that other formulations emerged. in parall ... | 2006 | 17095950 |
| synthesis and in vitro evaluation of the anti-viral activity of n-[4-(1h(2h)-benzotriazol-1(2)-yl)phenyl]alkylcarboxamides. | a series n-[4-(1h(2h)-benzotriazol-1(2)-yl)phenyl]alkylcarboxamides (8e-k, 9e-i, k, l) and their parent amines (5a-c and 6a-d) were prepared according to schemes (1 and 2). compounds were evaluated in vitro for cytotoxicity and antiviral activity against a wide spectrum of rna (positive- and negative-sense) viruses, like [bovine viral diarrhea virus (bvdv), yellow fever virus (yfv), coxsackie virus b (cvb-2), polio virus (sb-1), human immunodeficiency virus (hiv-1), respiratory syncytial virus ( ... | 2006 | 17105439 |
| beyond randomized controlled trials: a "real life" experience of respiratory syncytial virus infection prevention in infancy with and without palivizumab. | a population-based study of the impact of palivizumab on confirmed respiratory syncytial virus (rsv) hospitalizations over a 7-year period within and between two similar health regions . clinicians in calgary implemented palivizumab prophylaxis for high-risk infants during the last four rsv seasons; clinicians in edmonton did not. the two cities are part of a unified health care system and similar sociodemographics. infants <36 weeks (wk) of gestational age (ga) were identified. rsv prophylaxis ... | 2006 | 17058279 |
| [the anti-respiratory syncytial virus effect of active compound of glycyrrhiza gd4 in vitro]. | to study the effect on anti-respiratory syncytial virus of an active compound gd4 from glycyrrhiza in vitro. | 2006 | 17059011 |
| immunoglobulin treatment for respiratory syncytial virus infection. | respiratory syncytial virus (rsv) bronchiolitis and pneumonia hospitalize hundreds of thousands of infants every year. treatment is largely supportive therapy, (for example, oxygen, fluids and occasionally mechanical ventilation). ribavirin, an antiviral agent, is licensed for severe rsv infection, although systematic reviews find it of no benefit. passive protection against rsv can be achieved through monthly intramuscular injection of the humanized monoclonal anti-rsv antibody palivizumab (syn ... | 2006 | 17054220 |
| plasmacytoid dendritic cells limit viral replication, pulmonary inflammation, and airway hyperresponsiveness in respiratory syncytial virus infection. | plasmacytoid dendritic cells (pdc), as major producers of ifn-alpha, are thought not only to be pivotal in antiviral immunity, but also to limit allergic inflammation. in this study, we delineate the role of pdc in a mouse model of respiratory syncytial virus (rsv)-induced airway inflammation. bone marrow-derived pdc generated high levels of ifn-alpha upon rsv infection, and the percentage of pdc expressing mhc class ii and maturation-associated costimulatory molecules was increased. however, th ... | 2006 | 17056556 |
| excretion patterns of human metapneumovirus and respiratory syncytial virus among young children. | as respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) cause serious respiratory tract infections, the routes of transmission of these viruses are important to elucidate. we examined the modes of virus shedding and shedding duration of rsv and hmpv in young children. | 2006 | 17052968 |
| epidemiological profile and clinical associations of human bocavirus and other human parvoviruses. | human bocavirus (hbov) and parv4 are newly discovered human parvoviruses. hbov, which was first detected in respiratory samples, has a potential role in the development of human respiratory disease. the present study compared the frequencies, epidemiological profiles, and clinical backgrounds of hbov and parv4 infections with those of other respiratory virus infections, by evaluating diagnostic samples referred to the specialist virology laboratory (svl) at the royal infirmary of edinburgh (edin ... | 2006 | 17041855 |
| human metapneumovirus infection in hospitalized children with acute respiratory disease in korea. | human metapneumovirus (hmpv) is a recently isolated virus, mostly associated with acute lower respiratory infection in children, of which symptoms are similar to those of respiratory syncytial virus (rsv) infection. the aim of our study was to determine the frequency of hmpv in hospitalized children with acute respiratory tract disease in korea. nasal aspirates from hospitalized children with respiratory infections under 15 yr old between december 2003 and february 2005 were included in the stud ... | 2006 | 17043416 |
| prospects of rna interference therapy in respiratory viral diseases: update 2006. | respiratory viruses, such as influenza, parainfluenza and respiratory syncytial virus (rsv), claim millions of lives annually. at present, there is no completely effective vaccine or drug against these highly mutable rna viruses. passive antibody therapies for rsv, despite their limited application and staggering cost, enjoy a virtual monopoly in a multibillion-dollar global market. recently, however, pioneering discoveries have launched rna interference as a novel, nucleic acid-based therapy ag ... | 2006 | 17049013 |
| [detection of etiologic agents and antibiotic resistance in children with acute lower respiratory tract infection in wenzhou city]. | the etiology of acute lower respiratory tract infection (lrti) in children in wenzhou city remains poorly defined. this study investigated the etiological agents responsible for acute lrti and patterns of the antibiotic resistant bacterial pathogens in children with acute lrti from wenzhou city. | 2006 | 17052392 |
| antioxidant treatment ameliorates respiratory syncytial virus-induced disease and lung inflammation. | respiratory syncytial virus (rsv) is a major cause of lower respiratory tract infection in children. no treatment has been shown to significantly improve the clinical outcome of patients with this infection. recent evidence suggests that oxidative stress could play an important role in the pathogenesis of acute and chronic lung inflammatory diseases. we do not known whether rsv induces pulmonary oxidative stress and whether antioxidant treatment can modulate rsv-induced lung disease. | 2006 | 17008643 |
| modular alpha-helical mimetics with antiviral activity against respiratory syncitial virus. | a 13-residue peptide sequence from a respiratory syncitial virus fusion protein was constrained in an alpha-helical conformation by fusing two back-to-back cyclic alpha-turn mimetics. the resulting peptide, ac-(3-->7; 8-->12)-bicyclo-fp[kdefd][ksird]v-nh(2), was highly alpha-helical in water by cd and nmr spectroscopy, correctly positioning crucial binding residues (f488, i491, v493) on one face of the helix and side chain-side chain linkers on a noninteracting face of the helix. this compound d ... | 2006 | 17017810 |
| [the efficiency (cost-effectiveness) of palivizumab as prophylaxis against respiratory syncytial virus infection in premature infants with a gestational age of 32-35 weeks in spain]. | to evaluate the efficiency (cost-effectiveness) of palivizumab in preventing severe respiratory syncytial virus (rsv) infection in premature infants with a gestational age of 32-35 weeks (ga 32-35) and two or more risk factors (rf) in spain. | 2006 | 17020726 |
| [incidence and cost of hospitalizations for bronchiolitis and respiratory syncytial virus infections in the autonomous community of valencia in spain (2001 and 2002)]. | to assess the incidence and costs of hospitalizations for bronchiolitis and respiratory syncytial virus (rsv) infections the autonomous community of valencia, spain. | 2006 | 17020727 |
| the cell biology of acute childhood respiratory disease: therapeutic implications. | respiratory syncytial virus (rsv), the recently identified human metapneumovirus (hmpv), and the human parainfluenza viruses (hpivs), cause most cases of childhood croup, bronchiolitis, and pneumonia. influenza virus also causes a significant burden of disease in young children, although its significance in children was not fully recognized until recently. this article discusses pathogens that have been studied for several decades, including rsv and hpivs, and also explores the newly identified ... | 2006 | 17027618 |
| significant morbidity associated with rsv infection in immunosuppressed children following liver transplantation: case report and discussion regarding need of routine prophylaxis. | respiratory syncytial virus (rsv) is an important cause of lower respiratory tract infection in infants and young children. in immunocompromised children, rsv infection poses a serious health threat with significantly increased and prolonged virus shedding and the development of severe respiratory disease. we report two patients, eight months and 20 months of age, who were admitted with severe rsv infection two months and 10 months post-transplant respectively. major risk factors for severe infe ... | 2006 | 17032430 |
| prevalence of respiratory viruses, including newly identified viruses, in hospitalised children in austria. | the aim of this epidemiological study was to determine the prevalence of respiratory viruses, including new viruses, in hospitalised children in austria. two hundred fourteen nasopharyngeal samples from hospitalised children were tested for the presence of viruses using cell culture and pcr and/or viral antigen assays. the results revealed a parainfluenza virus 1 (piv1) outbreak that ended right before the onset of the influenza season, with nearly no overlapping, moderate respiratory syncytial ... | 2006 | 17036151 |
| respiratory syncytial virus f envelope protein associates with lipid rafts without a requirement for other virus proteins. | like many enveloped viruses, human respiratory syncytial virus (rsv) assembles at and buds from lipid rafts. translocation of the envelope proteins to these membrane subdomains is essential for production of infectious virus, but the targeting mechanism is poorly understood and it is not known if other virus proteins are required. here we demonstrate that f protein of rsv intrinsically targets to lipid rafts without a requirement for any other virus protein, including the sh and g envelope prote ... | 2006 | 17005642 |
| in vitro and in vivo fitness of respiratory syncytial virus monoclonal antibody escape mutants. | respiratory syncytial virus (rsv) is the only infectious disease for which a monoclonal antibody (mab) is used in humans. palivizumab (pz) is a humanized murine mab to the f protein of rsv. pz-resistant viruses appear after in vitro and in vivo growth of rsv in the presence of pz. fitness for replication could be a determinant of the likelihood of dissemination of resistant viruses. we assessed the fitness of two pz-resistant viruses (f212 and mp4). f212 grew less well in cell culture than the p ... | 2006 | 17005645 |
| alternate polypurine tracts affect rous sarcoma virus integration in vivo. | when the endogenous polypurine tract (ppt) of the rous sarcoma virus (rsv)-derived vector rsvp(a)z was replaced with alternate retroviral ppts, the fraction of unintegrated viral dna with the normal consensus ends significantly decreased and the retention of part of the ppt significantly increased. if the terminus of the u3 long terminal repeat (ltr) is aberrant, rsv integrase can correctly process and integrate the normal u5 ltr into the host genome. however, the canonical ca is not involved in ... | 2006 | 17005708 |
| [non-invasive ventilation in infants with severe infection presumably due to respiratory syncytial virus: feasibility and failure criteria]. | beneficial effect of continuous positive airway pressure (cpap) during non invasive ventilation (niv) has been reported in infants with respiratory syncytial virus (rsv) infection, but no study has analyzed the predictors of its failure. | 2006 | 16978849 |
| genetic analysis of resistance to soybean mosaic virus in j05 soybean. | soybean cultivar j05 was identified to be resistant to the most virulent strain of soybean mosaic virus (smv) in northeastern china. however, the reaction of j05 to smv strains in the united states of america is unknown, and genetic information is needed to utilize this germplasm in a breeding program. the objectives of this study were to determine the reaction of j05 to all us strains of smv (g1-g7), the inheritance of smv resistance in j05, and the allelic relationship of resistance genes in j ... | 2006 | 16990409 |
| serum antibody decay in adults following natural respiratory syncytial virus infection. | serum antibody decay following rsv infection in adults was examined to evaluate the durability of the immune response. twenty subjects with rsv infection and 10 subjects who remained rsv uninfected had blood samples obtained over 16-25 months analyzed by microneutralization assay and enzyme immunoassay. the mean titers of infected subjects rose approximately eightfold post-infection. the mean rate of antibody decline was -0.20 log 2 titer per month which led to a > or =fourfold drop in titer in ... | 2006 | 16998887 |
| comparison of multiplex pcr assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness. | the performances of four multiplex pcr (m-pcr) were compared to direct immunofluorescence assay (dfa) and huh7 cell culture for the detection of viruses in 263 children admitted to hospital with an acute respiratory illness. one hundred fifty (57.6%) nasal aspirates were found dfa-positive; 188 (72.3%) were found positive by both dfa and huh7 cell culture, and 242 (92%) were pcr-positive. the m-pcr detected 124 viruses which were not found by conventional methods: 68 rhinovirus, 17 human metapne ... | 2006 | 16998894 |
| detection of human coronavirus nl63, human metapneumovirus and respiratory syncytial virus in children with respiratory tract infections in south-west sweden. | two recently detected viruses, human metapneumovirus (hmpv) and coronavirus nl63 (hcov-nl63), have been associated with acute respiratory tract infections, particularly in young children. this study investigated the frequency of hmpv and hcov-nl63 infections in swedish children by screening 221 nasopharyngeal aspirates, collected between november 2003 and may 2005, from 212 children attending the paediatric department of a county hospital in sweden or submitted from local general practitioners. ... | 2006 | 17002608 |
| respiratory syncytial virus (rsv) evades the human adaptive immune system by skewing the th1/th2 cytokine balance toward increased levels of th2 cytokines and ige, markers of allergy--a review. | infection of infants in their first year of life, children and elderly people with the respiratory syncytial virus (rsv) endangers the life of the patient. an attempt to develop a formalin-inactivated rsv (fi-rsv) vaccine during the 1960s resulted in an aggravated infection in immunized children, leading to hospitalization, while infection of non-immunized children produced much milder symptoms. the reason for this remained an enigma, one which was gradually solved over the last decade by many r ... | 2006 | 16972040 |
| respiratory syncytial virus(rsv)-induced allergy may be controlled by il-4 and cx3c fractalkine antagonists and cpg odn as adjuvant: hypothesis and implications for treatment. | based on the hypothesis that respiratory syncytial virus (rsv) sg protein causes allergy in patients, it is suggested that treatment of rsv patients with antagonists of il-4 and fkn early in infection will prevent the increased level of il-4 in the serum. together with cpg odns that induce toll-like receptor 9(+) (tlr9(+)) plasmacytoid dendritic cells to release type i ifn-alpha and -beta will reactivate the inhibited th1 cells and the antiviral cytotoxic t leukocytes. in addition, binding of cp ... | 2006 | 16972041 |
| anti-neoplastic effect of chicken anemia virus vp3 protein (apoptin) in rous sarcoma virus-induced tumours in chicken. | the anti-neoplastic effect of chicken anemia virus vp3 protein (apoptin) was investigated in vitro in rous sarcoma virus (rsv)-transformed chicken embryo fibroblast (cef) cells and in rsv-induced tumours of specific-pathogen-free (spf) chicks in vivo. the apoptin gene was cloned in the pvax expression vector and in vitro expression of the recombinant vector pvax-cav-vp3 was confirmed. two groups of spf chicks, each containing ten chicks, were used. chicks in groups i and ii were inoculated with ... | 2006 | 16963752 |