Publications

[hantavirus emergence in the americas and in argentina].		1998	9674240
[pathogenesis, physiopathology, clinical manifestations and treatment of hantavirus pulmonary syndrome].		1998	9674241
[epidemiology of hantavirus pulmonary syndrome in argentina (1991-1997)].		1998	9674242
[epidemic outbreak of hantavirus pulmonary syndrome in argentina. molecular evidence of person to person transmission of andes virus].		1998	9674243
[reservoirs and genetic viral diversity].		1998	9674244
[hantavirus pulmonary syndrome. summary of the findings in argentina].		1998	9674245
[aids and the challenge of the emergent diseases. closing remarks].		1998	9674258
pathogenesis of a north american hantavirus, black creek canal virus, in experimentally infected sigmodon hispidus.	this report describes the first detailed analysis of the replication, persistence, and excretion of a north american hantavirus in its natural rodent reservoir. black creek canal virus was isolated from sigmodon hispidus (cotton rat) shortly after the identification of a hantavirus pulmonary syndrome (hps) case occurring in southern florida. six-week-old male cotton rats were inoculated subcutaneously with 1,000 tissue culture infectious doses. viral complementary rna (vcrna) was quantified as a ...	1998	9684629
automatic detection of conserved rna structure elements in complete rna virus genomes.	we propose a new method for detecting conserved rna secondary structures in a family of related rna sequences. our method is based on a combination of thermodynamic structure prediction and phylogenetic comparison. in contrast to purely phylogenetic methods, our algorithm can be used for small data sets of approximately 10 sequences, efficiently exploiting the information contained in the sequence variability. the procedure constructs a prediction only for those parts of sequences that are consi ...	1998	9685502
characterization of the nucleic acid binding properties of tomato spotted wilt virus nucleocapsid protein.	tomato spotted wilt tospovirus (tswv) is the type member of the plant-infecting viruses of the genus tospovirus in the family bunyaviridae. the three tswv rnas are encapsidated with nucleocapsid (n) protein to form ribonucleoprotein (rnp) which serves as the template for viral transcription and replication. regions of the open reading frame coding for the n protein on the small (s) rna were subcloned into pet protein expression vectors and expressed in escherichia coli bl21 (de3) cells. full-len ...	1998	9705250
antigenic characterization of hantaan and seoul virus nucleocapsid proteins expressed by recombinant baculovirus: application of a truncated protein, lacking an antigenic region common to the two viruses, as a serotyping antigen.	hantaan virus (htn) and seoul virus (seo) are members of the genus hantavirus in the family bunyaviridae and are causative agents of hemorrhagic fever with renal syndrome. the complete and truncated nucleocapsid proteins (np) of htn and seo were expressed by a recombinant baculovirus system. antigenic characterization of the np using monoclonal antibodies (mabs) indicated that the binding sites for the serotype-specific mabs were located between amino acids (aa) 155 and 429. a western blot assay ...	1998	9705385
from the centers for disease control and prevention. hantavirus pulmonary syndrome--colorado and new mexico, 1998.		1998	9707130
southwest cautious over hantavirus outbreak.		1998	9713524
host genes and infectious diseases.		1998	9716963
human dobrava hantavirus infections in estonia.	outbreaks of severe hemorrhagic fever with renal syndrome (hfrs) caused by dobrava hantavirus (dob) have been reported in albania, greece, and bosnia-herzegovina. a seroepidemiologic study conducted by the authors in estonia provided the first evidence of human dob infection in a baltic country. hantavirus infection was first confirmed in estonia in 1986 and believed related to puumala hantavirus (puu). to enable precise serotyping, serum samples were collected from 1728 healthy people in 11 ...	1998	9717928
[america hantaviruses: new data and implications].		1998	9718552
isolated abducens nerve palsy as a complication of haemorrhagic fever with renal syndrome.		1998	9719177
hantavirus pulmonary syndrome treated with inhaled nitric oxide.		1998	9726354
lack of serologic evidence for an association between hantavirus infection and end-stage renal disease.		1998	9730717
zoonoses and haemorrhagic fever.	virus zoonoses causing haemorrhagic fever have been recognized in three major families: arenaviridae, bunyaviridae and filoviridae. all are negative-stranded rna viruses, with genomes in two segments, three segments, or non-segmented, respectively. acquisition of haemorrhagic fever in man generally requires close contact with a vertebrate vector species, usually rodents, for the arenaviruses and bunyaviruses. in the case of filoviruses, the vector is currently unknown, but these viruses may infe ...	1998	9737374
hantaviruses: an emerging threat to human health?		1998	9742991
high prevalence of hantavirus infection in indian communities of the paraguayan and argentinean gran chaco.	serologic evidence of past infection with a sin nombre-like hantavirus(es) was demonstrated in 78 (40.4%) of 193 indians living in western paraguay and in 38 (17.1%) of 222 indians inhabiting the salta province of northern argentina. in both populations seroprevalence increased with age, with the most striking increase occurring at 18 years of age in the paraguayan population and at 35 years of age in the salta population. the peak prevalences in both populations (66.6% and 44.0%, respectively) ...	1998	9749641
ecology and demographics of hantavirus infections in rodent populations in the walker river basin of nevada and california.	to study the ecologic correlates of hantavirus in deer mice (peromyscus maniculatus), we sampled 114 sites in the walker river basin of nevada and california in 1995-1996. blood samples were tested for antibody to hantavirus, and a subset of samples was also tested for virus rna by reverse transcription-polymerase chain reaction. average prevalence of antibody-positive mice was 17%, with heavier males the most likely to be infected. antibody prevalence varied within repeatedly sampled sites from ...	1998	9749642
first reported case of hantavirus pulmonary syndrome in oklahoma.	hantavirus pulmonary syndrome (hps) is a condition of rapidly progressive pulmonary failure with a case-fatality rate of almost 50 percent. rodents serve as the reservoir for hantaviruses and human infection occurs primarily via aerosolized virus in rodent excreta. the rodent reservoir for the disease is widespread across oklahoma and the first case of hps has recently been confirmed in an oklahoma resident. physicians should suspect hps in a previously healthy person who develops a febrile illn ...	1998	9763765
hantavirus seropositivity in israeli patients with renal failure.	the hantavirus is known to cause hemorraghic fever with renal syndrome (hfrs), which is widely spread in europe and asia. several reports have shown an association of hantavirus antibody titers and the occurrence of renal dysfunction. from these observations, it appears that the virus is widely distributed, and different strains prevail in various areas. in the present work we studied 81 patients with end-stage renal-failure under hemodialysis (hd) treatment, 55 with mild to moderate renal failu ...	1998	9765032
[in the month of may, do whatever you please...].		1998	9775088
infectious agents and the etiology of chronic idiopathic diseases.	at the end of the nineteenth century, the field of microbiology was born, and the infectious nature of many previously unexplained diseases was illuminated as powerful new technology was applied. at the end of the twentieth century, the etiology of myriad chronic diseases remains unexplained. we have argued that many of these diseases have clinical, epidemiological, and pathological features that suggest a role for microbes in their pathogenesis. although definitive evidence of microbial disease ...	1998	9779355
nucleotide sequence and phylogenetic analysis of the medium (m) genomic rna segments of three hantaviruses isolated in china.	the medium (m) genome segment of hantaviruses (family bunyaviridae) encodes the two virion glycoproteins. g1 and g2, as a precursor protein in the complementary sense rna. we determined the nucleotide sequences of the m genome segments of three chinese hantavirus isolates, a hantaan-type (htn) virus designated a9 and two seoul-type (seo) viruses designated l99 and hb55, and compared them to those of other htn or seo viruses isolated in eastern asia. the m segment of a9 is 3616 nucleotides in len ...	1998	9784066
[anti-hantavirus antibodies in school children in salvador, bahia].	hantavirosis are emerging diseases in the americas. although considered rare in children, among the five cases diagnosed in brazil, one was in this age group. to know the serum-prevalence of hantavirosis in the pediatric group (schoolchildren) of low social economic level in salvador, bahia state, and to associate demographic, socio-economic and environmental factors to the serologic results, a standard questionnaire was applied and blood samples were collected from 379 students of two public sc ...	1998	9789441
a major outbreak of hemorrhagic fever with renal syndrome in the samara region, european russia.		1998	9795799
seroprevalence of antibodies to hantavirus in health care workers and other residents of southern argentina.		1998	9798052
hantavirus infection in the four corners region of usa in 1998.		1998	9802275
isolation and characterization of puumala hantavirus from norway: evidence for a distinct phylogenetic sublineage.	puumala (puu) hantavirus is the aetiological agent of nephropathia epidemica (ne), a mild form of haemorrhagic fever with renal syndrome, which occurs in fennoscandia, central europe and russia. in norway, ne-like disease has been reported since 1946 and about 50 cases are diagnosed annually; however, the causative agent has not been characterized. in this study, a virus originating from bank voles (clethrionomys glareolus) trapped near the town of eidsvoll (akershus county) was isolated and pas ...	1998	9820135
completion of the tula hantavirus genome sequence: properties of the l segment and heterogeneity found in the 3' termini of s and l genome rnas.	in this study the l segment and the 5' and 3' termini of the s, m and l segments of the prototype tula hantavirus (tul) were sequenced, thus completing the first determination of the genome sequence of a hantavirus that has not been linked to any human disease. the tul l segment comprises 6541 nt with one orf of 6459 nt in the antigenome sense. this orf potentially encodes a 2153 aa protein with a predicted molecular mass of 247 kda. the amino acid sequence includes all the motifs conserved in r ...	1998	9820136
a minority of seropositive wild bank voles (clethrionomys glareolus) show evidence of current puumala virus infection.	bank voles (clethrionomys glareolus) serve as the reservoir for puumala (puu) virus, the aetiologic agent of nephropathia epidemica. the animals are believed to be persistently infected and the occurrence of serum antibodies is usually taken as an evidence of active infection. we found serum antibodies to puu virus in 42 of 299 wild bank voles captured in a puu virus endemic area. puu virus rna was demonstrated in lung specimens of 11 of these 42 animals and in 2 of them antigen was also found. ...	1998	9825795
epidemiology of hantavirus infections in europe.		1998	9829467
hantavirus infection--new threats by an old virus.		1998	9829468
black creek canal virus infection in sigmodon hispidus in southern florida.	a total of 1,500 small mammals were collected and tested for antibodies cross-reactive to sin nombre virus (hantavirus: bunyaviridae) at 89 sites in a 1,600 km2 study area of southern florida. more than 95% of the 123 seropositive animals were cotton rats (sigmodon hispidus), suggesting infection by black creek canal virus, although seroreactive rattus rattus (5 of 294) and peromyscus gossypinus (1 of 39) also were captured. crude seroprevalence in s. hispidus was 11%. seroprevalence increased w ...	1998	9840584
emerging pathogens: the hong kong experience.		1997	9594216
[emerging infectious diseases--hantavirus infections].		1997	9480304
oligoryzomys flavescens (rodentia, muridae): gene flow among populations from central-eastern argentina.	in species acting as hosts of infectious agents, the extent of gene flow between populations is of particular interest because the expansion of different infectious diseases is usually related to the dispersal of the host. we have estimated levels of gene flow among populations of the sigmodontine rodent oligoryzomys flavescens, in which high titers of antibodies have been detected for a hantavirus in argentina that produces a severe pulmonary syndrome. enzyme polymorphism was studied by means o ...	1997	9465403
baculovirus expression of a human g2-specific, neutralizing igg monoclonal antibody to puumala virus.	we amplified by polymerase chain reaction the heavy- and light-chain antibody genes of a human hybridoma secreting a neutralizing, igg monoclonal antibody to the g2 protein of puumala virus. the heavy- and light-chain genes were cloned and sequenced and the deduced amino acids were aligned with those of other human antibodies to identify the constant and variable regions. the genes were cloned into the baculovirus plasmid transfer vector pacuw51 such that the heavy-chain and light-chain genes we ...	1997	9281505
[viral haemorrhagic fever].	viral haemorrhagic fever denotes various kinds of febrile illness caused by certain viruses which often presents with bleeding tendency and occasionally shock. out of these, the four maladies, lassa fever, ebola haemorrhagic fever, marburg haemorrhagic fever and crimean-congo haemorrhagic fever which are endemically present in africa or eastern europe, are known to be such diseases with high man-to-man communicability. these four haemorrhagic fevers are, therefore, designated as special conditio ...	1997	9283226
hantavirus transmission in the united states.	in 1996, investigation of a hantavirus pulmonary syndrome (hps) outbreak in southern argentina found evidence of person-to-person transmission of a hantavirus. the infection control ramifications of this finding led to this review of hantavirus epidemiology in the united states; the review suggests that sin nombre virus infection is rarely, if ever, transmitted from person to person and that existing guidelines for prevention of hps remain appropriate for north america.	1997	9284382
population dynamics of the deer mouse (peromyscus maniculatus) and sin nombre virus, california channel islands.	hantavirus pulmonary syndrome, first documented in 1993, is caused by sin nombre virus (snv), which is carried by the peromyscus species. in 1994, high snv antibody prevalence was identified in deer mice from two california channel islands. we sampled two locations on three islands to estimate mouse population density and snv prevalence. population flux and snv prevalence appear to vary independently.	1997	9284383
polarized entry and release in epithelial cells of black creek canal virus, a new world hantavirus.	black creek canal (bcc) virus is a newly identified hantavirus from florida which is carried by the cotton rat (sigmodon hispidus) and is associated with hantavirus pulmonary syndrome (hps). we have investigated the interaction of bcc virus with polarized epithelial cells to examine whether entry and release of this virus occur at specific plasma membrane domains. the polarized vero c1008 monkey kidney cell line was grown on permeable filters and infected with bcc virus either through the apical ...	1997	8995636
the investigation of emerging and re-emerging viral diseases: a paradign.	emerging virus infections are defined as previously nonthreatening viruses that can decimate new populations by finding fresh hosts and vectors--often with the help of humans who introduce new species into virgen environment, several etiologic agents of these diseases, some of the interacting factors that contribute to their development and the role of molecular medicine in their understanding is discussed.	1997	9419931
the evolution of hantaviruses.	hantaviruses exist in most regions of the world. the many different strains identified thus far have widely divergent roles in human disease and infect a wide range of rodent hosts. the sequence data available for the genomes of these viruses allows us to study indirectly the evolutionary patterns of the hantaviruses. in this paper, we describe relationships among the m genomic segments of hantaviruses, and attempt to relate these to the evolutionary relationships of the virus' rodent hosts.	1997	9037623
rapid and specific detection of sin nombre virus antibodies in patients with hantavirus pulmonary syndrome by a strip immunoblot assay suitable for field diagnosis.	to develop a rapid antibody test for sin nombre hantavirus (snv) infection for diagnosis of hantavirus pulmonary syndrome (hps) in field settings where advanced instrumentation is not available, a strip immunoblot assay bearing four immobilized antigens for snv and a recombinant nucleocapsid protein antigen of seoul hantavirus (seov) was prepared. the snv antigens included a full-length recombinant-expressed nucleocapsid (n) protein (rn), a recombinant-expressed g1 protein (residues 35 to 117), ...	1997	9041397
pathogenetic and clinical aspects of the renal involvement in hemorrhagic fever with renal syndrome.	hemorrhagic fever with renal syndrome is the most common clinical manifestation of hantavirus infection. the main target organ is the kidney, resulting in an interstitial hemorrhagic nephritis and sometimes acute tubular necrosis. the pathogenesis is still largely unknown, but several recent studies indicate an important role for immune mechanisms including increased expression of cytokines, for example, tumor necrosis factor. immunohistochemical studies of kidney biopsies have revealed deposits ...	1997	9044447
hantavirus pulmonary syndrome in wisconsin.	in the spring of 1993 an outbreak of a new illness caused by a new pathogen was identified in the southwestern united states. this infection struck relatively young, healthy individuals, was characterized by fever, myalgias, respiratory failure, and a high mortality rate. this illness was caused by a new hantavirus and has been termed hantavirus pulmonary syndrome (hps). the virus is carried by rodents, shed in saliva, urine, and feces. human infection occurs through inhalation of aerosolized vi ...	1997	9046232
hantavirus infection.		1997	9048125
[hantavirus: an ignored pandemic in switzerland?].	as numerous publications attest, knowledge on hantaviruses progressed rapidly since the isolation of the agent of korean hemorrhagic fever in 1978. this trend was even enhanced after the recognition of a previously unknown hantavirus that caused an outbreak of acute respiratory distress syndromes in the southwestern united states in 1993. hantaviruses are carried by rodents worldwide and may cause severe diseases in humans. hantavirus pulmonary syndrome in the new world and hemorrhagic fever wit ...	1997	9082534
phylogenetic methods come of age: testing hypotheses in an evolutionary context.	the use of molecular phylogenies to examine evolutionary questions has become commonplace with the automation of dna sequencing and the availability of efficient computer programs to perform phylogenetic analyses. the application of computer simulation and likelihood ratio tests to evolutionary hypotheses represents a recent methodological development in this field. likelihood ratio tests have enabled biologists to address many questions in evolutionary biology that have been difficult to resolv ...	1997	9092465
phage-displayed peptide targeting on the puumala hantavirus neutralization site.	we have selected ligands for puumala hantavirus, the causative agent of nephropathia epidemica, from a seven-amino-acid peptide library flanked by cysteines and displayed on a filamentous phage. to direct the selection to areas on the virus particle which are essential for infection, phages were competitively eluted with neutralizing monoclonal antibodies specific for the viral glycoproteins. the selected phage populations were specific for the same sites as the antibodies and mimicked their fun ...	1997	9094664
hantavirus pulmonary syndrome.	the objective of this report is to summarize the medical literature relevant to the epidemiology, diagnosis, treatment, and control of hantavirus pulmonary syndrome (hps). the english language literature was searched from january 1993 through march 1995, manually and using medline, including conference proceedings and reference lists. we selected relevant articles on clinical aspects of hps. hps is a newly described illness that progresses through three phases: prodromal, cardiopulmonary, and co ...	1997	9097374
new hantaviruses causing hantavirus pulmonary syndrome in central argentina.		1997	9100632
common questions about hantavirus pulmonary syndrome.		1997	9103926
puumala hantavirus genome in patients with nephropathia epidemica: correlation of pcr positivity with hla haplotype and link to viral sequences in local rodents.	reverse transcription-pcr was used to analyze specimens from 20 finnish nephropathia epidemica (ne) patients hospitalized during the period from october 1994 to january 1995. blood and/or urine sediment specimens from seven patients were found to be positive for the genome sequences of puumala hantavirus (puu). pcr positivity of the specimens from the patients correlated well with the hla-drb1*0301 and hla b8 alleles, which previously were shown to associate with severe courses of ne. genetic an ...	1997	9114386
serological diagnosis of hantavirus infections by an enzyme-linked immunosorbent assay based on detection of immunoglobulin g and m responses to recombinant nucleocapsid proteins of five viral serotypes.	worldwide, hantaviruses cause more than 100,000 human infections annually. rapid and accurate methods are important both in monitoring acute infections and for epidemiological studies. we and others have shown that the amino termini of hantavirus nucleocapsid proteins (ns) are sensitive tools for the detection of specific antibodies in hantavirus disease. accordingly, we expressed truncated ns (amino acids 1 to 117) in escherichia coli from the five hantaviruses known to be pathogenic to man; ha ...	1997	9114393
hantavirus in argentina.		1997	9121542
patterns of association with host and habitat: antibody reactive with sin nombre virus in small mammals in the major biotic communities of the southwestern united states.	the distribution and prevalence of antibody reactive with sin nombre virus were determined in mammals in biotic communities of the southwestern united states. small mammals (n = 3,069) of 69 species were trapped in nine communities from lower sonoran desert to alpine tundra. antibody was found in rodents from all communities (overall prevalence = 6.3%); prevalence was lowest at the altitudinal and climatic extremes (0.4% in desert and 2.0% in alpine tundra). antibody occurred in 11% of 928 deer ...	1997	9129529
single amino acid substitutions in puumala virus envelope glycoproteins g1 and g2 eliminate important neutralization epitopes.	two monoclonal antibody escape virus mutants (mars), rescued from a human mab to glycoprotein 2 (g2) and a bank vole monoclonal antibody (mab) directed to glycoprotein 1 (g1) of puumala virus, strain sotkamo, were produced by using a combination of neutralization tests and antigen detection. the mars and the original virus were analyzed by nucleotide sequencing and the responsible mutations were defined and characterized. the g1 mutation was found to constitute an a to t nucleotide substitution, ...	1997	9140197
dobrava hantavirus in estonia: does the virus exist throughout europe?		1997	9149707
[further study of hantavirus circulation in the russian federation].	lung specimens of 1514 small mammals of 35 species captured in 1991-1995 at 9 territories of russia were tested in elisa for virus antigens of hemorrhagic fever with the renal syndrome (hfrs). the antigens were detected in lung specimens of clethrionomys glareolus, microtus arvalis, microtus gregalis, microtus fortis, arvicola terrestris, apodemus agrarius, micromys minutus, and sorex sp., well known as hantavirus reservoirs, captured in the vologda, yaroslavl, saratov, astrakhan, and chita regi ...	1997	9182404
incidence, etiologic pathogens, and diagnostic testing of community-acquired pneumonia.	determination of the etiologic pathogens of community-acquired pneumonia has been problematic because of the lack of reliable rapid laboratory diagnostic tools as well as the controversy concerning diagnostic criteria. in the studies reviewed here, a specific pathogen was identified in 39% to 88% of patients. streptococcus pneumoniae remains the most common cause of community-acquired pneumonia. depending on the demographics of the study, between 2% to 43% of cases have been attributed to legion ...	1997	9193852
atypical pathogen pneumonia.	the term atypical pathogens has been applied in recent years to chlamydia pneumoniae, mycoplasma pneumoniae, and the various species of legionella. the incidence of pneumonia caused by these pathogens has increased with the development of specific diagnostic techniques. atypical pathogen community-acquired pneumonia demonstrates a broad spectrum of severity from a mild disease not requiring hospitalization to adult respiratory distress syndrome necessitating mechanical ventilation. the clinical, ...	1997	9193855
hantaviruses: four years after four corners.	hantavirus pulmonary syndrome is an acute, often fatal, febrile illness causing rapid pulmonary failure and cardiovascular instability. since first described in 1993, considerable progress has been made in identifying the hantaviruses responsible for the disease and in defining its epidemiologic and diagnostic features.	1997	9194804
hantaviruses: a global disease problem.	hantaviruses are carried by numerous rodent species throughout the world. in 1993, a previously unknown group of hantaviruses emerged in the united states as the cause of an acute respiratory disease now termed hantavirus pulmonary syndrome (hps). before than, hantaviruses were known as the etiologic agents of hemorrhagic fever with renal syndrome, a disease that occurs almost entirely in the eastern hemisphere. since the discovery of the hps-causing hantaviruses, intense investigation of the ec ...	1997	9204290
an unusual hantavirus outbreak in southern argentina: person-to-person transmission? hantavirus pulmonary syndrome study group for patagonia.	hantavirus pulmonary syndrome is a rodent-borne zoonosis first recognized in the united states in 1993. person-to-person transmission has not been reported; however, in the outbreak of 20 cases reported here, epidemiologic evidence strongly suggests this route of transmission.	1997	9204298
seroepidemiologic studies of hantavirus infection among wild rodents in california.	a total of 4,626 mammals were serologically tested for antibodies to sin nombre virus. all nonrodent species were antibody negative. among wild rodents, antibody prevalence was 8.5% in murids, 1.4% in heteromyids, and < 0.1% in sciurids. of 1,921 peromyscus maniculatus (deer mice), 226 (11.8%) were antibody positive, including one collected in 1975. the highest antibody prevalence (71.4% of 35) was found among p. maniculatus on santa cruz island, off the southern california coast. prevalence of ...	1997	9204301
the hantaviruses of europe: from the bedside to the bench.	in europe, hantavirus disease can hardly be called an emerging zoonosis; it is rather a rediscovered disease. since 1934 an epidemic condition with primarily renal involvement has been described in sweden. nowadays, hundreds to thousands of cases per year are registered in fennoscandia, fluctuating with the numbers of the specific arvicoline-rodent reservoir, the red bank vole, which carries the main european serotype, puumala (puu). in the early 1980s, the rat-transmitted serotype, seoul (seo), ...	1997	9204306
virus zoonoses--a long-term overview.		1997	9208195
a major antigenic domain of hantaviruses is located on the aminoproximal site of the viral nucleocapsid protein.	hantavirus nucleocapsid protein has recently been shown to be an immunodominant antigen in hemorrhagic with renal syndrome (hfrs) inducing an early and long-lasting immune response. recombinant proteins representing various regions of the nucleocapsid proteins as well as segments of the g1 and the g2 glycoproteins of hantavirus strains cg18-20 (puumala serotype) and hantaan 76-118 have been expressed in e. coli. the antigenicity of these proteins was tested in enzyme immunoassays and immunoblots ...	1997	9208453
ecological monitoring helps researchers study disease in environmental context.		1997	9218651
experimental evaluation of rodent exclusion methods to reduce hantavirus transmission to humans in rural housing.	an experimental assessment of methods to reduce rodent infestations in rural housing was conducted in yosemite national park, california, sequoia/kings canyon national parks, california, and shenandoah national park, virginia. during pretreatment surveys, nearly all (63 of 68) selected units had past or ongoing rodent activity inside. active infestations were found in 58.8% of the units. peromyscus spp. represented 91.2% of all animals caught inside housing units. despite little harborage, roden ...	1997	9158040
playing in a scrapyard and acute renal failure.		1997	9164319
assessment of occupational risk for hantavirus infection in arizona and new mexico.	differentiating occupational exposure from other potential domestic or recreational exposure(s) for sin nombre virus (snv) infection is an epidemiologic challenge. interviews on work-related activities were conducted, and serum specimens were obtained from 494 workers in arizona and new mexico. these workers may have been exposed to rodents and rodent excreta at work, but their primary occupation did not require rodent contact (national park service [n = 193]; navajo agricultural product industr ...	1997	9172092
community structure and prevalence of hantavirus infection in rodents: a geographic division of the enzootic area in far eastern russia.	the results of an extensive rodent trapping effort throughout the southern part of far eastern russia and hantavirus antigen screening of tissues were used to develop a multifaceted approach for the geographic division of the enzootic territory of hantavirus. four species of rodents (apodemus agrarius, apodemus peninsulae, microtus fortis, and clethrionomys rufocanus) comprised 88.5 percent of 10,595 captured rodents and 94.1 percent of 996 antigen-positive animals. rodent fauna and the prevalen ...	1997	9221739
[the hantavirus pulmonary syndrome (its etiology, epidemiology, pathogenesis, clinical manifestations and the diagnosis of a new infectious disease)].		1997	9229840
hantavirus antibodies in new york.		1997	9230017
high prevalence of hantavirus antibodies in bank voles (clethrionomys glareolus) captured in the vicinity of households afflicted with nephropathia epidemica.	puumala virus, the causative agent of nephropathia epidemica (ne), occurs endemically in europe and is spread mainly by the bank vole (clethrionomys glareolus). in the vicinity of each of four households afflicted with ne, we studied rodents with regard to population density and prevalence of puumala virus-specific antibodies. for each case area, a control area was randomly selected 10 km away, without regard to the presence of human settlement. during 6,000 trap nights, 328 rodents were caught, ...	1997	9230802
human-to-human transmission of hantaviruses.		1997	9284808
[approaches to selecting a strain of hantavirus for developing an inactivated brain vaccine].	four strains isolated from patients with hemorrhagic fever with the renal syndrome and from field mice, natural hosts of hantavirus, were examined in order to choose a functionally active strain fit for the development of inactivated hantavirus brain vaccine. virulent strain pm-10508/89 isolated from mouse lungs was chosen. the strain was highly virulent for newborn mice and rats: 100% of infected mice aged up to 24 h and 75% of rats developed an acute lethal infection upon challenge. the incuba ...	1997	9297337
dobrava hantavirus outbreak in russia.		1997	9298001
puumala and dobrava viruses cause hemorrhagic fever with renal syndrome in bosnia-herzegovina: evidence of highly cross-neutralizing antibody responses in early patient sera.	hantavirus infection was diagnosed serologically by mu-capture igm and igg elisas in hemorrhagic fever with renal syndrome (hfrs) patients admitted to tuzla hospital, bosnia-herzegovina. the results indicated that more than one hantavirus caused the outbreak. to address the question of which hantavirus serotypes were involved, sequentially drawn sera were analyzed by focus reduction neutralization test (frnt) for antibodies against puumala, hantaan, dobrava, and seoul hantaviruses. the data reve ...	1997	9298732
[the ecological aspects of the epidemiology of hemorrhagic fever with renal syndrome in the south of the far east in russia].	the data on the relationship between the epizootic process in the population of mice (the source of hantavirus infection) and the seasonal manifestations of morbidity in hemorrhagic fever with renal syndrome (hfrs) in the southern region of the russian far east are presented. the asynchronous course of the epizootic process in the population of rodents causes irregularity in the spread of hfrs in the region and periodicity in the appearance of foci. seasonal rises in hfrs morbidity coincide with ...	1997	9304320
an outbreak of hantavirus pulmonary syndrome in western paraguay.	during an investigation of hantavirus pulmonary syndrome (hps) in paraguay in 1995, sera from persons with hps-like illness, houshold contacts of confirmed hps case-patients, and a sample of the area residents were analyzed by elisa for antibodies to sin nombre virus (snv). rodent serosurveys and analysis of precipitation records were also conducted. twenty-three of 24 available probable cases were snv antibody-positive, 17 of whom were ill between july 1995 and january 1996. four (14.8%) of 27 ...	1997	9311636
rio mamore virus: genetic characterization of a newly recognized hantavirus of the pygmy rice rat, oligoryzomys microtis, from bolivia.	human hantavirus disease occurs throughout much of south america. the rodent hosts and the specific etiologic agent(s) are largely unknown, but many reported cases occurred within the habitation ranges of oryzomine rodents (rice rats). we have identified a genetically novel hantavirus (rio mamore virus [rm]) of the pygmy rice rat oligoryzomys microtis in bolivia. the complete sequence of the small (s) genome and the partial sequence of the medium (m) genome are described. this virus is closely r ...	1997	9311652
a colorimetric pcr-enzyme immunoassay to identify hantaviruses.	hantaviruses cause two serious human diseases: hantavirus pulmonary syndrome and hemorrhagic fever with renal syndrome. at least nine hantaviruses are known to be pathogenic for humans and numerous others, with unknown disease potential, have been detected in rodents. assays to quickly identify specific hantaviruses would be useful both for clinical diagnosis and in risk assessment studies.	1997	9316733
epitope mapping by phage display: random versus gene-fragment libraries.	we present a comparative study on epitope mapping of four monoclonal antibodies directed against four different antigens using alternative phage display techniques and peptide scanning: mab215 reacts with the largest subunit of rna polymerase ii, mabbp53-11 with the tumor suppressor protein p53, mabgdo5 with the hantaan virus glycoprotein g2 and mabl13f3 with the hantaan virus nucleocapsid protein. epitopes were determined (i) by gene-fragment phage display libraries, constructed by dnasei diges ...	1997	9328567
role of electron microscopy in interstitial lung disease.	although electron microscopy no longer enjoys the important role in the diagnosis of interstitial lung diseases that it had in the 1960s and 1970s, it remains an important adjunct in the differential diagnosis of certain pulmonary diseases. examples include various manifestations of systemic lupus erythematous pneumonitis, in which the presence of tubuloreticular structures and electron-dense deposits are useful for diagnosis; immotile cilia disorders, in which qualitative and now quantitative s ...	1997	9331535
the first human isolate of puumala virus in scandinavia as cultured from phytohemagglutinin stimulated leucocytes.	a virus isolate was recovered from blood leucocytes of a patient with nephropathia epidemica (ne). leucocytes were isolated from edta-blood by dextran sedimentation and cultured on monolayers of vero e6 cells in the presence of phytohemagglutinin (pha) in roller tubes during the first 72 hours of incubation followed by rolling culture for three weeks in total. thereafter the first subculture was done in a plastic flask and afterward at at least 6 week intervals. antigen was first detected after ...	1997	9334926
puumala virus and two genetic variants of tula virus are present in austrian rodents.	puumala and tula viruses are hantaviruses found in europe and are associated with the rodents clethrionomys glareolus and microtus arvalis, respectively. puumala virus is associated with the human disease nephropathia epidemica. in austria, ten clinically diagnosed cases of nephropathia epidemica, presumably caused by puumala virus infection, have been reported but not virologically confirmed [leschinskaya et al., 1991; aberle et al., 1996]. to identify the hantaviruses that are present in austr ...	1997	9334930
effectiveness of recorded messages to communicate the risk of acquiring hantavirus pulmonary syndrome.	to determine the effectiveness of a recorded information line in communicating health risk during the emergence of a new disease, hantavirus pulmonary syndrome (hps), in the edmonton area and to study the accuracy of recall of information about hantavirus among the general public.	1997	9336097
hantavirus pulmonary syndrome -- chile, 1997.	hantavirus pulmonary syndrome (hps) was first recognized in chile in october 1995; through july 1997, nine cases had been identified in the country. however, during august 1-october 8, 1997, a total of 12 persons with hps, including two family clusters, were recognized. a collaborative investigation is under way to determine the magnitude of this outbreak, the associated rodent reservoir for hantaviruses, and the major risk factors for human infection. this report summarizes the preliminary resu ...	1997	9338456
hantavirus pulmonary syndrome in the americas.		1997	9341305
glomerular basement membrane antibodies in hantavirus disease (hemorrhagic fever with renal syndrome).	the prevalence of antibodies against glomerular basement membrane (gbm) antigens in sera from 47 patients with serologically verified hantavirus (puumala serotype) infection were investigated. antibodies were measured by immunoassays using as antigen a crude human gbm preparation and the nc1 portion of type iv collagen (goodpasture antigen), respectively. seventy-seven percent of the patients had igm antibodies against the non-goodpasture glomerular basement membrane (non-gp gbm) in their acute ...	1997	9342483
isolation, genetic diversity, and geographic distribution of bayou virus (bunyaviridae: hantavirus).	bayou hantavirus, previously implicated in human hantavirus pulmonary syndrome in louisiana, was isolated from a rice rat (oryzomys palustris) captured in georgia. the presence of antibody among rice rats captured throughout the southeastern united states and the extent of diversity among the genetic variants of bayou viruses suggest that the rice rat is the most likely natural reservoir of the virus and that both virus and host have probably co-evolved for some years.	1997	9347961
nephropathia epidemica infection during first trimester of pregnancy--normal fetal outcome.		1997	9351417