Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
|---|
| cell-mediated immunity in marek's disease virus-infected chickens genetically selected for high and low concentrations of plasma corticosterone. | lines of chickens genetically selected for high (hpc)-and low-plasma corticosterone (lpc) titers were assessed for cell-mediated immunity during marek's disease tumorigenesis. lymphocyte transformation (using phytohemagglutinin and 2,4-dinitrofluorobenzene-bovine serum complex) and mitogen-induced cellular cytotoxicity were evaluated with peripheral blood leukocytes. chickens from the lpc line showed a greater lymphocyte transformation response than those from the hpc line. all tumor-bearing chi ... | 1980 | 7362128 |
| selection for virulence and organ-specific metastasis of herpesvirus-transformed lymphoma cells. | the marek's disease virus-transformed, non-producer, lymphoma cell line, mdcc-rpi, was selected by sequential transplantation to produce a highly malignant variant, mdcc-ali. this is evidenced gy an 18-fold decrease in ld50. the new cell line has an increased ability to form metastatic lesions in a distribution which mimics the natural disease. further selections for organ-specific metastasis were undertaken with the isolation of two cell lines, mdcc-al2, selected for liver metastasis, and mdcc- ... | 1980 | 7390658 |
| isolation and serological differentiation of a herpesvirus from bobwhite quail (colinus virginianus, l. 1758). | an infectious agent was isolated from the liver of bobwhite quails (colinus virginianus, l. 1758). the agent was sensitive to chloroform and its multiplication was inhibited by 5-iodine-2-deoxy-uridine. it passed filters with a pore diameter of 220 nm and more but not 100 nm filters. electron microscopic examination revealed numerous nucleocapsids with hollow capsomeres and few enveloped particles in the supernatant fluids of infected cultures. the nucleocapsids were calculated to have 162 capso ... | 1980 | 7447711 |
| independence of chicken major histocompatibility antigens and tumor-associated antigen on the surface of herpesvirus-induced lymphoma cells. | capping of chicken major histocompatibility (mhc) antigens on normal thymus, spleen, and peripheral blood leukocytes was demonstrated, although mhc antigens appeared to be present on only 15 to 18% of normal thymus cells. mhc antigen capping also occurred on cells from a marek's disease herpesvirus-induced transplantable lymphoma (mdct-nym1). capping of a marek's disease tumor-associated surface antigen (matsa) could be induced on mdct-nym1 lymphoma cells as well as on cells of two marek's disea ... | 1980 | 7000703 |
| cell-mediated immunity in marek's disease: cytotoxic responses in resistant and susceptible chickens and relation to disease. | two experiments were conducted to study the cell-mediated cytotoxicity of peripheral blood leukocytes (pbl) from chickens inoculated with marek's disease virus (mdv) against a marek's disease-derived lymphoblastoid cell line (msb-1) and to associate the cytotoxicity with incidence of disease. in experiment i, moderately susceptible random-bred, specific-pathogen-free chickens were inoculated with mdv (group 1), vaccinated with a herpesvirus of turkeys (hvt) and inoculated with mdv (group 2), vac ... | 1980 | 6445171 |
| in vitro suppression of t-cell mitogenic response and tumor cell proliferation by spleen macrophages from normal chickens. | adherent cells isolated from spleen of normal specific pathogen-free chickens inhibited mitogen-induced blastogenesis of autochthonous, syngeneic, or allogeneic lymphocytes. the adherent cells were also inhibitory to in vitro proliferation of cells of a rapidly dividing tumor line, mdcc-msb-1, derived from a lymphoma induced by marek's disease virus. the effector cell of suppression of both lymphoprolifrative functions appeared to be a macrophage because the suppressive activity of adherent cell ... | 1980 | 6447112 |
| spontaneous cell-mediated cytotoxicity (scmc) in various mammalian species and chickens: selective reaction pattern and different mechanisms. | cultured cell lines derived from donors of various species served as 75se- or 51cr-labelled targets in microcytotoxicity assays. as in human donors, considerable spontaneous cell-mediated cytotoxicity (scmc) was exhibited by peripheral blood lymphoid effector cells from healthy chickens, mink, swine, cattle, horses and tigers. although all target lines tested could be lysed in scmc, there was no 'general scmc target' for all species or all individuals of one species. the selectivity, the kinetic ... | 1980 | 9537048 |
| anti-viral immunity against marek's disease virus infected chicken kidney cells. | the mechanism of immunity conferred by herpesvirus of turkeys (hvt) was investigated using marek's disease virus (mdv)-infected chicken kidney cell (ckc) cultures as target cells. peripheral blood lymphocytes (pbl) from normal or hvt-vaccinated chickens killed mdv-infected target cells in the presence of anti-mdv or anti-hvt serum, whereas normal lymphocytes and hvt-sensitised lymphocytes themselves had no specific cytotoxic effect. cell-mediated cytotoxicity of pbl from hvt-vaccinated chicken w ... | 1979 | 18770425 |
| serological characterisation of marek's disease tumour-associated surface antigens on marek's disease lymphoma cells and on cell lines derived from marek's disease lymphomas. | antisera produced in rabbits against marek's disease lymphoma cells and against four lymphoma-derived cell lines (hprs lines 1 and 2, msb-1 and rpl-1) appeared, after extensive absorption with normal chicken cells, to react specifically by indirect immunofluorescent staining with antigens unique to marek's disease tumour cells. the antigens present on the four cell lines were shown by antiserum titration and absorption tests to be related but not identical. the possibility is discussed that cell ... | 1979 | 18770440 |
| atheroarteriosclerosis induced by infection with a herpesvirus. | atheroarteriosclerosis closely resembling that in humans was induced in normocholesterolemic and hypercholesterolemic chickens by infection with marek's disease herpesvirus (mdv). four comparably sized groups of chickens were used. each group was initially fed a diet relatively poor in cholesterol. group i and ii were inoculated intratracheally at 2 days of age with mdv. at 15 weeks, one group of virus-infected chickens (group ii) and one group of uninfected controls (group iv) were fed a 2% cho ... | 1979 | 382868 |
| nonprotective and temperature-sensitive variants of marek's disease vaccine viruses. | 1979 | 214607 | |
| enhanced oncornavirus expression in marek's disease tumors from specific-pathogen-free chickens. | an oncornavirus was recovered from cell cultures of kidney tumors from specific-pathogen-free chickens inoculated with marek's disease herpesvirus (mdhv). the mdhv inoculum was free of infectious avian leukosis virus (alv). direct examination of a variety of tissues from mdhv-inoculated chickens demonstrated increased levels of alv-specific rna compared to tissues from diluent-inoculated (control) chickens. dna from cultured kidney tumor cells annealed to an alv complementary dna probe at the sa ... | 1979 | 216833 |
| further study on the three-dimensional structure of the core of marek's disease virus and herpesvirus of turkey. | three-dimensional structures of the core of marek's disease virus and herpesvirus of turkey were examined by the tilting apparatus of an electron microscope. various types of the core found in the infected cells were considered to represent developmental stages of the viruses. the basic structure of the core consisted of a toroid surrounding a cylindrical mass, which was clearly demonstrated by tilting the core in two directions. a cylindrical mass spooled by more than two toroids, which seemed ... | 1979 | 218531 |
| no homology detectable between marek's disease virus (mdv) dna and herpesvirus of the turkey (hvt) dna. | the relation of four different strains of mdv and two strains of hvt was analyzed by gel electrophoresis of viral dna digested by various restriction endonucleases and by filter hybridization of viral dna with complementary rna. the four mdv strains showed fragment patterns completely different from those of hvt upon digestion of the viral dna with bam h i, eco r i, hind iii, hpa, i, and xho and separation of fragments on agarose gels. the cleavage patterns of the four mdv strains showed great s ... | 1979 | 219323 |
| transcription of the marek's disease virus genome in a nonproductive chicken lymphoblastoid cell line. | 1979 | 219594 | |
| minor dna homology between herpesvirus of turkey and marek's disease virus? | 1979 | 219602 | |
| effects of vaccination for marek's disease on growth of a marek's-virus-induced transplantable lymphoma in syngeneic and allogeneic chickens. | a study was made of the effects of vaccination with turkey herpesvirus on the growth of tumors following inoculation of lymphoma cells induced by marek's disease virus (mdv). the cells used were from a transplantable mdv-induced lymphoid tumor (mdt-198) maintained by passaging in syngeneic hosts. vaccination did not prevent progressive tumor growth or alter subsequent mortality among syngeneic recipients of mdt-198 cells. with allogeneic recipients, however, vaccination had a significant protect ... | 1979 | 486007 |
| serum protein changes in chickens with experimental marek's disease. | serum protein alterations in chickens contact-exposed to marek's disease virus (mdv) were studied by crossed immunoelectrophoresis. total protein values of infected chickens were initially, i.e. in the 4th week, low and later not significantly changed from those of the controls. in all infected chickens a significant increase was seen in the igg components. three of 6 infected chickens showing a prolonged period of clinical illness presented the most pronounced changes in other serum proteins, m ... | 1979 | 523311 |
| pathogenicity of a marek's disease virus for chickens vaccinated with graded doses of herpesvirus of turkeys. | six different groups of one-day-old chickens were vaccinated with herpesvirus of turkeys (hvt) vaccine at doses of 0, 4, 16, 64, 256 and 1,024 plaqueforming units. at 9 days of age they were challenged with an acute strain of marek's disease herpesvirus (mdhv). gross and microscopic lesions in nerves and gonads were investigated and combined for quantitative expression of mdhv pathogenicity. including the microscopic lesions improved the accuracy of the hvt doses/mdhv response. the qualitative a ... | 1979 | 546406 |
| demonstration of chicken fetal antigen (cfa) on normal splenic lymphocytes, marek's disease lymphoblastoid cell lines and other neoplasms. | chicken fetal antigen (cfa) was detected on normal splenic lymphocytes and a direct relationship was observed between the percentage of cfa-positive cells and the age of the donor. the fetal antigen was also detected on lymphoblastoid tumor cells and cell lines induced by known avian oncogenic viruses (marek's disease virus and avian leukosis virus), and on spontaneously occurring adenocarcinoma cells. the fetal antigen appears to be distinct from marek's disease tumor-associated surface antigen ... | 1979 | 90665 |
| the role of the macrophages in marek's disease: in vitro and in vivo studies. | macrophages form s- and k-strain leghorn chickens, susceptible and restant to marek's disease (md) respectively, were studied to determine the macrophage contribution to the dynamics of md infection, tumorigenesis and genetic resistance to this disease. in vitro studies demonstrated that macrophages from bothstrains were similar in their responses toward jm strain of marek's disease virus (mdv) and jm-1 tumor cells. macrophages were observed to phagocytize jm virus, but the interiorized virus wa ... | 1979 | 222692 |
| lymphomas resembling lymphoid leukosis in chickens inoculated with reticuloendotheliosis virus. | chickens inoculated as embyros or at hatching with the chick syncytial strain of reticuloendotheliosis virus developed a high incidence of lymphoid neoplasms between the 17th and 43rd weeks of age, involving principally the liver and bursa of fabricius. on the basis of organ distribution, latent period, pathology and surface igm production, the lymphomas closely resembled those of lymphoid leukosis. one inoculated chicken developed a myxosarcoma. no tumors were observed in uninoculated controls. ... | 1979 | 222694 |
| expression of genetic resistance to an oncogenic herpesvirus at the target cell level. | 1979 | 223067 | |
| infection studies on a reticuloendotheliosis virus contaminant of a commercial marek's disease vaccine. | reticuloendotheliosis virus (rev) was isolated in cell cultures from commercial marek's disease (herpesvirus of turkeys) vaccine and re-isolated from the organs of vaccinated chickens. runting and feathering abnormalities were produced when 1-day-old specific pathogen free chickens were inoculated with rev. histopathological lesions in infected chickens were hypoplasia of the thymus, bursa and spleen, and inflammation of the proventriculus, kidneys and liver. serological responses to rev were de ... | 1979 | 223539 |
| macrophage restriction of marek's disease virus replication and lymphoma cell proliferation. | macrophages are shown to restrict the replication of marek's disease virus (mdv) and isotope uptake by spleen cells from chickens bearing marek's disease (md) tumors. the titer of virus from duck embryo fibroblasts (def) co-cultivation with mdv-spleen cells pretreated to deplete marcophages was 4- to 18-fold higher than with untreated cells. treated mdv-spleen cells increased isotope uptake by 2-fold. these restrictive activities are attributable to macrophage regulation of cell proliferation. | 1979 | 224109 |
| induction of virus-neutralizing antibody by glycoproteins isolated from chicken cells infected with a herpesvirus of turkeys. | the production of virus-induced proteins in chicken embryo fibroblast cells infected with a herpesvirus of turkeys was studied. it was found that glycoproteins isolated from membrane-rich fractions of infected cells by affinity chromatography using concanavalin a induced neutralizing and precipitating antibody in rabbits and chickens. after analytical electrophoresis, such isolates were found to contain three polypeptide bands of between 100 x 10(3) and 120 x 10(3) molecular weight not present i ... | 1979 | 225277 |
| transcription of the marek's disease virus genome in virus-induced tumors. | transcription of the marek's disease virus (mdv) genome in tumor tissues from mdv-infected chickens has been studied by analyzing the hybridization kinetics of (3)h-labeled mdv dna with unlabeled rna extracted from these tissues. lymphoid tumors of ovary, spleen, liver, and kidney contained mdv genomes, but the virus-specific rna sequences were transcribed from less than 15% of the viral dna. a virus nonproductive lymphoblastoid cell line, designated mkt-1, has been established from a kidney lym ... | 1979 | 225531 |
| [susceptibility of japanese quail cells to virus infections in vitro and in vivo]. | 1979 | 225762 | |
| effects of avian reovirus on marek's disease (md). i. suppression of md development. | the effects of an avian reovirus on the development of acute marek's disease (md) were studied by exposing chickens to reo-w isolant of avian reovirus before infecting them with an oncogenic md herpesvirus (mdhv). four separate trials were made in a similar manner, 2 trials with an experimental line of white leghorn (wsu-vs) highly susceptible to md, and 2 trials with a commercial strain of meat-type (c-mt) chicken. md mortality and/or incidence of gross md lesions were consistently reduced by p ... | 1979 | 226049 |
| complement-dependent antibody cytotoxicity test of chicken antibody with duck complement used against cells of a marek's disease lymphoma-derived cell line (msb-1). | complement-dependent antibody cytotoxicity (cdac) against cells of a lymphoblastoid cell line (msb-1) derived from marek's disease lymphoma was investigated in a chicken antibody system using complements from several animal species. cytotoxicity was seldom observed when rabbit, guinea pig, or chicken complements were used in the presence of hyperimmune chicken serum against msb-1. with duck complement, however, cytotoxicity was always observed. therefore, duck complement appears to be suitable f ... | 1979 | 226054 |
| pathogenesis of marek's disease-distribution of viral antigens in infected chickens as detected by immuno-peroxidase test. | 1979 | 226476 | |
| a comparison of titration methods for marek's disease vaccines. | 1979 | 226553 | |
| depression of vaccinal immunity to marek's disease by infection with reticuloendotheliosis virus. | the effect of infection with low-virulence, tissue culture-propagated strains of reticuloendotheliosis virus (rev) on protective vaccinal immunity against marek's disease (md) lymphomas was investigated. vaccinated chickens inoculated at hatching with greater than 10(4) focus-forming units of rev and challenged with md virus were poorly protected against md lesion development as indicated by protective indices of 53 to 79% for strain cs (p less than 0.05) and 42 to 49% for strain t (p less than ... | 1979 | 227800 |
| inhibitory effects of macrophages against marek's disease virus plaque formation in chicken kidney cell cultures. | inhibition of plaque formation by marek's disease virus (mdv) in chicken kidney cell cultures was investigated with the use of peritoneal exudate cells (pec) from chickens. pec from mdv-infected white leghorn chickens inhibited the formation of mdv plaques, whereas the inhibitory effect of pec from chickens vaccinated with herpesvirus of turkey (hvt) or pec from normal chickens was very weak. however, pec from either normal chickens or hvt-vaccinated chickens inhibited the mdv plaque formation i ... | 1979 | 228107 |
| replication of turkey herpesvirus and marek's disease virus in chick embryo skin organ culture. | turkey herpesvirus (hvt) and an attenuated marek's disease virus (mdv) replicated in organ cultures of chick embryo skin as assessed by immunofluorescence and/or electron microscopy. hvt-specific immunofluorescent antigen was detected in the feather follicle epithelium (ffe) and in the surface layer of the skin epidermis. electron microscopy of infected explants revealed herpes-type cytopathology. immature particles of both viruses appeared first in the nucleus. oval or horseshoe-shaped non-enve ... | 1979 | 228368 |
| marek's disease lymphoma of chicken and its causative herpesvirus. | 1979 | 228727 | |
| turkey herpesvirus and marek's disease virus. a comparative appraisal. | 1979 | 228905 | |
| studies on genetic resistance of chickens to marek's disease--a review. | 1979 | 228906 | |
| comparative pathogenesis studies with oncogenic and nononcogenic marek's disease viruses and turkey herpesvirus. | an apparently nononcogenic marek's disease virus (sb-1) and turkey herpesvirus could be readily isolated from spleen, bursa of fabricius, thymus, and peripheral blood lymphocytes of chickens beginning 4 to 6 days after inoculation, but unlike infections with two isolates of oncogenic marek's disease virus (jm-10 and cu-2), virus replication in these cells was rare, and necrosis in the organs was essentially absent. splenic enlargement was observed regularly during the first 4 to 11 days after in ... | 1979 | 229747 |
| intracellular state of marek's disease virus dna in two tumour-derived chicken cell lines. | 1979 | 230282 | |
| comparative studies on marek's disease virus and herpesvirus of turkey dnas. | dna of marek's disease virus (mdv) was compared to that of herpes virus of turkey (hvt). centrifugation of the two virus dnas in neutral glycerol and cscl density gradients showed that the mdv genome was slightly larger than that of hvt and that the buoyant density (1.705 g/ml) of mdv dna in cscl gradients was slightly lower than that (1.707 g/ml) of hvt dna. mdv and hvt dnas were digested with either ecori or hindiii restriction endonuclease and analysed by 0.5% agarose gel electrophoresis. the ... | 1979 | 230299 |
| increased susceptibility of leukemia-infected chickens to marek's disease. | intramuscular (i.m.) injection of rav-49 into 1--3 day-old chickens infected with marek's disease herpes virus (mdhv) by contact led to exceedingly high rate of marek's disease (md) incidence. according to the data obtained in three experiments 52 out of 108 (48.1%) chickens fell ill in this group. while in the group uninfected with rav-49 but contactly infected with mdv-kekava there were only 11 out of 102, (10.7%) incidences. simultaneous inoculations of rav-49 and mdv had no effect on md morb ... | 1979 | 230419 |
| [avian viral infection with a neoplastic evolution: marek's disease]. | 1979 | 230584 | |
| marek's disease in chickens: correlation of marek's disease with nuclear-inclusion formation in feather-follicle epithelium. | the dynamics of nuclear-inclusion (ni) formation in feather-follicle epithelium of chickens inoculated with marek's disease virus (with or without prior immunization with turkey herpesvirus) could be divided into two patterns: 1) transient ni formation at the initial stage postchallenge; and 2) persistent ni formation. incidence of marek's disease was closely correlated with the dynamics of ni formation. active ni formation recurred in chickens showing pattern 2 and was closely related to the in ... | 1979 | 230801 |
| low incidence of lymphoid tumors in chickens continuously producing endogenous virus. | a flock of 258 male and 243 female chickens of a cross of regional poultry research laboratory lines 15b and 7(2) were kept in a filtered-air positive-pressure house and observed for tumors from 100 to more than 729 days of age. these birds produced high titers of a subgroup e endogenous virus from the middle of the embryonic incubation period through the end of the experiment. no neoplasms were observed in the males. the females had two neoplasms indistinguishable from lymphoid leukosis and thr ... | 1979 | 230806 |
| [the molecular biology of herpes viruses: (part ii) dna structure analysis and latent viral genomes of ebv, mdv, h. saimiri, cmv etc. (author's transl)]. | 1979 | 231281 | |
| effect of culture temperature on the production of marek's disease virus antigens in a chicken lymphoblastoid cell line. | 1979 | 231431 | |
| establishment of marek's disease lymphoblastoid cell lines from chickens with babk of b blood groups. | 1979 | 231432 | |
| marek's disease virus and herpesvirus of turkey noninfective to chickens, obtained by repeated in vitro passages. | the relation between the passage level of marek's disease virus, c2 strain, and of herpesvirus of turkey (hvt), o1 strain, in cell culture and the level of the serological response of chickens to these viruses was examined. in both cases the immune response of chickens to these viruses decreased with increase in the number of in vitro passages of virus. virus was not recovered from chickens inoculated with hvt highly passaged in vitro, which had become a high producer of cell-free virus in vitro ... | 1979 | 231437 |
| increased agglutinability and haemadsorption of marek's disease hprs line 1 lymphoblastoic cells after concanavalin a treatment. | the hprs line 1 lymphoblastoid cells derived from an ovarian lymphoma induced by marek's disease virus in chickens displayed increased agglutinability and haemadsorption when incubated with con a. the increase was proportional to the concentration of con a used. the same con a-untreated cells and normal chicken lymphocytes incubated with con a exhibited no increase in agglutinability and haemadsorption, however. | 1979 | 231521 |
| chronological study of ultrastructural changes in the peripheral nerves in marek's disease. | a chronological study was made of the ultrastructural changes in peripheral nerves following inoculation of 1-day-old chicks with a neurogenic strain of marek disease virus. no virus particles were found in nerves. cellular infiltration of nerves was detected as early as 5 days after inoculation and by 3 weeks some nerves contained proliferative lesions which possessed many of the ultrastructural features characteristic of normal, reactive lymphoid tissue. about 4 weeks after inoculation, coinci ... | 1979 | 231745 |
| influence of rabies virus on the oncogenesis in marek's disease. | 1979 | 232370 | |
| marek's disease tumor-associated surface antigen (matsa) in resistant versus susceptible chickens. | the appearance of matsa-bearing cells in the spleens of genetically susceptible (p-line) and resistant (n-line) chickens followed similar patterns at 4 and 6 days postinoculation (pi), with 4 to 10% of the cells positive in indirect fluorescent-antibody tests. levels of matsa-positive cells at 10, 14, and 21 days pi continued at 6-8% in p-line birds but dropped from about 8% to below 1 or 2% in n-line birds. this pattern was seen also in virus-isolation rates from the same spleen samples. viral ... | 1979 | 232656 |
| loss of cell-associated marek's disease vaccine titer during thawing, reconstitution, and use. | tests of the effects of mismanagement practices observed in hatcheries showed that very subtle deviations from the explicit directions accompanying marek's vaccine can cause dramatic losses of vaccine potency. such mismanagement was found to result in 14 to 97% loss of vaccine titer. it appears that procedures used in hatcheries for the handling of cell-associated marek's vaccine should be closely monitored on a continuous basis to prevent a gradual drift from correct handling. | 1979 | 232657 |
| pathogenesis of marek's disease; effect of immunization with inactivated viral and tumor-associated antigens. | inactivated marek's disease virus-infected chicken kidney cells and inactivated msb-1 lymphoblastoid marek's disease tumor cells were used to immunize chickens as virus- and tumor-associated antigens, respectively. immune and nonimmune birds were then challenged by exposure to live virulent marek's disease virus. both vaccines protected significant numbers of chickens (p less than 0.05) against subsequent tumor development, although viral antigen appeared superior to tumor antigen. after challen ... | 1979 | 232690 |
| marek's disease in comparative medicine. | 1979 | 233254 | |
| contrasting characteristics of marek's disease herpesvirus isolated from chickens with and without avian leukosis virus infection. | marek's disease herpesvirus (mdhv) isolated from chickens free of naturally occurring avian leukosis virus (alv) infection produced characteristic foci in both chicken embryo fibroblast (cef) and chicken kidney cell (ckc) cultures. mdhv-a, which was extracted from the feather follicle epithelium of chickens naturally infected with alv, did not induce cytologic changes in cef cultures, but did cause focus formation in ck cultures. alv was detected in mdhv-a, but not in mdhv preparations. mdhv-a ( ... | 1978 | 204600 |
| inhibition of herpesvirus replication and herpesvirus-induced deoxyribonucleic acid polymerase by phosphonoformate. | phosphonoformate was found to be an inhibitor of the deoxyribonucleic acid polymerase induced by the herpesvirus of turkeys. the apparent inhibition constants were 1 to 3 mum. phosphonoformate was also able to block the replication in cell culture of marek's disease herpesvirus, the herpesvirus of turkeys, and herpes simplex virus. it was as effective as phosphonoacetate. phosphonoformate was not an effective inhibitor of a phosphonoacetate-resistant mutant of the herpesvirus of turkeys nor of i ... | 1978 | 206200 |
| an improved method for extracting cell-free herpesviruses of marek's disease and turkeys from infected cell cultures. | sonic extraction of cell-free marek's disease herpesvirus (mdhv) and turkey herpesvirus (hvt) from infected cell cultures was improved by incorporating sorbitol in the suspending media. yields of cell-free virus of virulent mdhv were significantly increased with 10% sorbitol added to spga-edta buffer. avirulent strains of mdhv and hvt were respectively readily extracted with spga-edta and spga as the suspending medium, and extraction of their cell-free viruses was moderately improved by adding s ... | 1978 | 206250 |
| spontaneously occurring lymphoproliferative disease in ducks. | a naturally occurring lymphoproliferative disease was observed in a domestic flock of muscovy ducks. gross lesions were observed only in one of three ducks necropsied (in the small intestine). microscopic lesions consisted of mononuclear cell infiltrates composed primarily of reticuloendothelial cells, mature lymphocytes, and plasma cells in livers, spleens, small intestines, peripheral nerves, and brains. antibodies to reticuloendotheliosis virus were detected in sera of four ducks, whereas all ... | 1978 | 206252 |
| sensitivity and specificity of the fluorescent antibody technique for detection of infectious laryngotracheitis virus. | the specificity of a fluorescent conjugate to infectious laryngotracheitis virus was examined using chick trachea organ culture or tissue sections infected with other avian viruses (adenovirus, infectious bronchitis, poxvirus, reovirus, newcastle disease virus, marek's disease virus, avian encephalomyelitis and infectious bursal agent) or mycoplasma gallisepticum. confirmation of virus replication in these preparations was obtained by either 1) demonstration of virus titre increase or 2) demonst ... | 1978 | 206327 |
| [significance of virus-neutralizing antibodies in marek's disease of chickens. 1. immunity control following marek's vaccination with turkey herpes virus (thv)]. | 1978 | 207505 | |
| pathogenesis of marek's disease in chickens with maternal antibody. | histopathological lesions were studied in chickens with maternal antibody against md virus at 14-day intervals after their exposure by contact to hprs-16 of md virus in the first-day of life. the first lesions occured 14 days after infection in the liver, kidney, heart, brain and the sciatic nerves. relatively, the most expressive changes were observed between 56th and 70th day after infection. lesions were characterized as light to heavy infiltrations formed by immature and mature pleomorphic m ... | 1978 | 208481 |
| stimulation of chicken spleen cell mitogenic response with turkey herpesvirus vaccine against marek's disease. | 1978 | 208875 | |
| in vitro establishment of marek's disease herpesvirus-transformed productive and nonproductive lymphoblastoid cell lines. | the establishment of two marek's disease herpesvirus-transformed lymphoblastoid cell lines (jmv-1 and jm-1) is reported. cocultivation with chicken embryo fibroblasts, attempts to rescue marek's disease virus from kidney cell cultures, and immunofluorescent staining show that jmv-1 is a nonproductive line and jm-1 is a productive line. | 1978 | 208974 |
| virus-induced atherosclerosis. | of four groups of chickens, two (groups i and ii) were infected with mdv and two were not (groups iii and iv). groups i and iii were fed diets low in lipid, and groups ii and iv were fed cholesterol-supplemented diets. striking grossly visible atherosclerotic lesions were seen in large coronary arteries, aortas, and major aortic branches of infected normocholesterolemic and hypercholesterolemic chickens (groups i and ii). in contrast, grossly visible atherosclerotic lesions were not seen in unin ... | 1978 | 209124 |
| vaccination immunity to selected diseases in chickens fed the androgen analog mibolerone. | chickens fed the androgen analog mibolerone during the first 7 weeks of life regress their bursa of fabricius but can be properly immunized by vaccination against avian pathogens of major economic importance such as newcastle disease virus, infectious laryngotracheitis virus, avian encephalomyelitis virus, infectious bronchitis virus, fowl pox virus, marek's disease virus, and pasteurella multocida, the pathogen causing fowl cholera. these findings on immunocompetence to infectious agents are im ... | 1978 | 209430 |
| a comparative pathogenesis of two mareks disease virus isolates. | the pathogenesis of the jm and ga isolates of marek's disease virus (mdv) infection was compared by studying the sequential tissue distribution of mdv associated antigens with gross and microscopic lesions in infected chickens. with both mdv isolates the initial sites of viral replication were the lymphoid organs, skin, and featers. both the ga and jm isolates were viscerotropic as demonstrated by the presence of mdv antigens with gross and microscopic changes occurring in several visceral organ ... | 1978 | 209437 |
| [testing the immunogenicity of the dermal antigen in marek's disease virus]. | an experiment was performed to study the immunogenicity of the dermal antigen of marek's disease virus, extracted from the skin of 30-day-old chickens, infected with marek's disease virus on the first day of life. three kinds of samples were tested: (1) dermal antigen centrifuged at 10 000 g per 0.5 h, (2) dermal antigen centrifugated at 10 000 g per 0.5 h and 100 000 g per 1 h, (3) dermal antigen treated like sample (2) and partly purified by deae-cellulose chromatography. samples (1) and (2) w ... | 1978 | 209603 |
| biochemical evidence of the nonintegrated status of marek's disease virus dna in virus-transformed lymphoblastoid cells of chicken. | 1978 | 209622 | |
| [immunogenic activity of vaccines against marek's disease]. | 1978 | 210559 | |
| [interaction of marek's disease and rous sarcoma viruses in a mixed infection in chicks]. | 1978 | 210560 | |
| suppression and enhancement of mitogen response in chickens infected with marek's disease virus and the herpesvirus of turkeys. | the kinetics of phytohemagglutinin (pha) response of peripheral blood lymphocytes from chickens infected with oncogenic marek's disease (md) virus (mdv) or nononcogenic herpesvirus of turkeys (hvt) was studied with a whole blood microassay. at about 7 days after inoculation, a depression in pha response was observed in mdv-inoculated resistant line n or susceptible line 7(2) chickens and in hvt-inoculated line 7(2) chickens. all chickens initially regained their pha responsiveness. susceptible c ... | 1978 | 211085 |
| demonstration of marek's disease tumor-associated surface antigen in chickens infected with nononcogenic marek's disease virus and herpesvirus of turkeys. | the presence of marek's disease tumor-associated surface antigen (matsa) was demonstrated on spleen cells from p-line chickens inoculated 5--6 days earlier with herpesvirus of turkeys and sb-1 (a nononcogenic marek's disease virus). antisera against matsa expressed on five marek's disease lymphoblastoid cell lines were able to recognize the matsa present on sb-1-infected spleen cells. no viral membrane antigens and only a low incidence of viral internal antigens could be demonstrated. | 1978 | 211242 |
| fused rocket and rocket immunoelectrophoresis adapted for demonstration and quantitation of the a antigen of marek's disease virus-infected cell cultures. | preliminary results have shown that the rocket immunoelectrophoresis technique can be used as a method for quantitation of the a antigen of marek's disease virus-infected cell cultures. | 1978 | 211484 |
| duration of vaccinal immunity against marek's disease. | specific vaccinal immunity against marek's disease (md) virus was measured by a short-term challenge test in which chickens vaccinated with turkey herpesvirus (hvt) and control chickens at various ages up to 40 weeks were inoculated with virulent md virus and evaluated 2 weeks later for microscopic lesions and md viremia. vaccinal immunity induced by high (2,900 pfu) and low (29 pfu) doses of hvt persisted without detectable reduction through the 40th week in line 151 chickens, thus indicating t ... | 1978 | 211999 |
| lymphoproliferative diseases of fowl: characterization of transplantable g-b1 marek's disease tumor cells in culture. | a transplantable marek's disease tumor, derived and maintained through in vivo passage in syngeneic g-b1 chickens, was established in cell culture and characterized. the cells (gbt cells) grew in suspension and appeared morphologically similar to other marek's lymphoblastoid lines except for prominent nucleoli in gbt cells. the number of chromosomes increased during in vitro cultivation from near triploid to subtetraploid. chromosome no. 3 was abnormally short. the gbt cells retained surface b1 ... | 1978 | 212000 |
| development of cell-mediated immunity to marek's disease tumor cells in chickens inoculated with marek's disease vaccines. | chickens inoculated with herpesvirus of turkeys or with apathogenic or attenuated vaccine strains of marek's disease virus (mdv) developed a t-cell-mediated immune response to marek's disease (md) tumor cells. this immune response was detected in a 4-hour 51cr-release assay in which effector cells obtained from spleens of vaccinated chickens were reacted with 51cr-labeled target cells of an md lymphoblastoid cell line (msb-1). the cytotoxic effector cells generated by the vaccine viruses had cha ... | 1978 | 213610 |
| specific restriction of avian sarcoma viruses by a line of transformed lymphoid cells. | 1978 | 213875 | |
| studies on the mechanism of vaccinal immunity to marek's disease. | 1978 | 93038 | |
| [immunological aspects of marek's disease in chickens with maternal antibodies]. | the dynamics of the production of immunoprecipitation antibodies to marek's disease virus was studied in the serum of chickens with maternal antibodies in relation to the occurrence of the immunoprecipitation antigens of marek's disease virus in feather follicles. one-day-old chickens were infected by the contact method with marek's disease virus. the first occurrence of immunoprecipitation antigen was detected on the 14th day after infection and this occurrence persisted throughout the experime ... | 1978 | 99864 |
| resistance to marek's disease. effect of corynebacterium parvum and marek's tumor cell vaccines on tumorigenesis in chickens. | a study of nonspecific stimulation of the avian immune system with corynebacterium parvum and specific stimulation with marek's tumor cell vaccines revealed that nonspecifically stimulated outbred white leghorn-type cockerels had higher incidences of tumors than did controls. a study of tumor cell cytotoxicity of sera from marek's disease virus exposed birds indicated that humoral factors may play some role in tumor resistance. | 1978 | 24754 |
| marke's disease herpesviruses. iii. purification and characterization of marek's disease herpesvirus b antigen. | sera from chickens naturally infected with marek's disease herpesvirus (mdhv) form preciptin lines with at least two immunologically distinct soluble antigens designated mdhv-a and mdhv-b. partial purification and characterization of the glycoprotein mdhv-a antigen was previously reported. mdhv-b was found predominantly in the sonically treated extracts of infected cells, in contrast to the predominantly extracellular mdhv-a. analysis of these extracts from 14cglucosamine-labeled cells by immuno ... | 1978 | 29134 |
| report of iabs committee on avian product standardization. | the international association of biological standardization (iabs) organized an international symposium in august 1973 on the "requirements for poultry virus vaccines" at lyon (france). the proceedings of this symposium were subsequently published as "developments in biological standardization", volume 25. at the conclusion of this symposium, it was resolved that an avian biological products standardization committee should be established, this committee including four subcommittees, each under ... | 1978 | 38158 |
| characterization of an apparently nononcogenic marek's disease virus. | a new isolate of marek's disease virus (mdv) was described. this virus, sb, and a clone, sb-1, differed from pathogenic isolates in in vitro growth characteristics as described for other apathogenic isolates. serologically, as with other apathogenic isolates, sb could be distinguished from pathogenic mdv and the avirulent turkey herpesvirus. sb failed to induce lesions characteristic of marek's disease (md) during a 6- to 11-week experimental period. also, sb was nononcogenic in immunosuppressed ... | 1978 | 76680 |
| a phosphonoacetate-resistant mutant of herpesvirus of turkeys. | a phosphonoacetate (pa)-resistant mutant of the herpesvirus of turkeys (hvt) was isolated and characterized. the mutant of hvt resistant to pa (hvtpa) replicated in duck embryo fibroblast (def) culture in media containing 300 microgram pa/ml, whereas the replication of the wild type of hvt (hvtwt) was completely inhibited in def culture in media containing 100 microgram pa/ml. the hvtpa was distinct from the hvtwt in plaque morphology, but was indistinguishable antigenically and showed in vitro ... | 1978 | 76681 |
| herpesviruses and cancer. | 1978 | 78013 | |
| density-gradient separation of spleen-cell subpopulations from marek's disease virus-infected chickens. | ficoll gradient separation of spleen-cell suspensions has shown that depressed t-cell mitogen reactivity in cultures of jm-vs and md cells results from selective depletion of mitogen-responsive t-cells. | 1978 | 312761 |
| pathogenesis of marek's disease: early appearance of marek's disease tumor-associated surface antigen in infected chickens. | marek's disease tumor-associated surface antigen (matsa) appeared in different lymphoid tissues of p-line chickens soon after they were infected with bc-1 strain of marek's disease virus. matsa-bearing cells first appeared in spleens by 5 days post infection (pi) and were observed through a 21-day experimental period at mean levels varying from 3.8 to 21.9% of the total cells examined. lower percentages of matsa-bearing cells were observed in the thymus, in the bursa of fabricius, among peripher ... | 1978 | 357748 |
| effectiveness of cell-free or cell-associated turkey herpesvirus vaccine against marek's disease in chickens as influenced by maternal antibody, vaccine dose, and time of exposure to marek's disease virus. | 1978 | 219826 | |
| in vitro inactivation of cell-free marek's disease herpesvirus by immune peripheral blood lymphocytes. | the effect of peripheral blood lymphocytes (pbl) on cell-free marek's disease virus (mdv) was studied in vitro in pbl from intact or embryonal bursectomized (bx) chicks infected with the apathogenic sb-1 isolate of mdv. the pbl were harvested purified and were mixed, 200 or 2,000 to 1, with cell-free ga-5 of mdv. virus survival was assayed after incubation for 1 hour at 36 c by the standard assay for cell-free mdv. pbl from intact but not bx chickens were able to inactivate virus. when the effec ... | 1978 | 219833 |
| status of endogenous avian rna tumor virus in marek's disease lymphoblastoid cell lines and susceptibility of those lines to exogenous rna tumor viruses. | three lymphoblastoid cell lines from marek's disease (md) tumors, two md virus (mdv) producer lines (msb-1 and hprs-1), and a nonproducer line (rpl-1) were studied for the expression of avian leukosis sarcoma (als) viruses. the msb-1 line was free of all known endogenous expressions, including replicating virus (rav-o), group-specific (gs) antigens, and chick helper factor (chf). the rpl-1 and hprs-1 were positive for gs antigens and chf. the rpl-1 and msb-1 lines showed no evidence of an exogen ... | 1978 | 219836 |
| altered biological and biochemical properties of a phosphonoacetate-resistant mutant of herpesvirus of turkeys. | a phosphonoacetate (pa)-resistant mutant of the herpesvirus of turkeys (hvt) was isolated and characterized. the mutant (hvtpa) replicates in growth medium containing 300 microgram/ml of pa and shows in vitro temperature sensitivity at 41 degrees c (its 37 degrees c/41 degrees c efficiency of replication is about 5). hvtpa replicates poorly in chickens and fails to provide complete protection against mdv challenge. the hvtpa-induced dna polymerase has an apparent inhibition constant for pa 10 ti ... | 1978 | 221331 |
| effect of cyclic nucleotides on the response of cells to infection by various herpesviruses. | the effect of varying the intracellular cyclic nucleotide concentration of mouse cells infected with hsv-1 or of duck embryo fibroblasts infected with marek's disease virus has been studied. it has been shown in each case that agents that elevate the intracellular camp levels increase the yield of infectious virus or viral dna, but inhibit cell division. cgmp enhances synthesis of viral dna but allows continued synthesis of cell dna. | 1978 | 221343 |
| identification and partial purification of two ebv-associated dna polymerases. | virally induced dna polymerases have been demonstrated in cells infected with a variety of herpesviruses. these include herpes simplex virus (weissbach et al., 1973), marek's disease virus (boezi et al., 1974), equine herpesvirus (allen et al., 1977), and cytomegalovirus (huang, 1975a). recently a new iododeoxyuridine (iudr)-induced intracellular dna polymerase in an epstein-barr virus (ebv)-producing hybrid cell line has also been reported (miller et al., 1977). we present evidence that there a ... | 1978 | 221345 |
| transformation of lymphoid cells by herpesviruses: a review with special reference to the phenotypic properties of transformed cells. | 1978 | 221350 | |
| non-protective variants of marek's disease vaccine viruses. | two variant marek's disease-vaccine viruses failed to induce protective immune responses in chickens and replicated poorly in vivo. one of these, mdv/200d, was defective for growth at 41 degrees c, and clones from this stock had 38 degrees c/41 degrees c plating ratios as high as 10(4). the basis for the poor in vivo replicative ability was not determined. | 1978 | 221372 |
| transfection studies in vitro and in vivo with isolated marek's disease virus dna. | for tranfection studies in vitro and in vivo, infectious marek's disease virus (mdv) dna was isolated from purified virus particles. its sedimentation coefficient in neutral sucrose gradients was found to be 53 +/- 2s and the buoyant density in caesium chloride corresponded to 1.707 g/cm3. the virus dna was also analysed by using the restriction endonucleases ecori and hindiii. high molecular-weight mdv dna precipitated by the calcium phosphate technique was shown to be infectious in cultivated ... | 1978 | 221387 |
| expression of marek's disease virus in producer and non-producer lymphoblastoid cell lines. | continuous in vitro propagation of a producer marek's disease (md) lymphoblastoid cell line reduced the number of cells producing virus antigens and the inducibility of these antigens to 5-iododeoxyuridine (iudr) but did not change the oncogenicity of the cells for chickens. in contrast, propagation of a non-producer md cell line did not change the non-productivity of the cell line, but decreased the transplantability of the cells. the md lines were free of replicating avian leukosis sarcoma vir ... | 1978 | 221388 |
| cytotoxicity of lymphocytes from chickens with marek's disease (md) to md tumour cell lines. | 1978 | 221408 |