Publications
| Title | Abstract | Year(sorted descending) Filter | PMID Filter |
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| microdialysis assessment of percutaneous penetration of ketoprofen after transdermal administration to hairless rats and domestic pigs. | the study was performed to evaluate the percutaneous penetration of ketoprofen after transdermal administration using a microdialysis technique in pigs, in comparison with rats. ketoprofen release from patches was determined by analysis of the remaining drug content after application to hairless rats and pigs. skin and knee joint penetration of ketoprofen was tested by microdialysis, and recovery was determined by retrodialysis. residual rates in hairless rats and pigs were 68.1 -¦ 1.6% and 81.7 ... | 2011 | 21397690 |
| validation of an automated method for salivary alpha-amylase measurements in pigs (sus scrofa domesticus) and its application as a stress biomarker. | the aim of the current study was to validate an automated spectrophotometric method for salivary alpha-amylase measurement in pigs and evaluate its possible application as a noninvasive stress biomarker. the analytical validation included intra- and interassay precision, linearity under dilution, and limit of detection. in addition, to study the possible use of salivary alpha-amylase as a possible stress marker, 12 crossbred growing pigs of 3-4 months of age were subjected to restraint stress by ... | 2011 | 21398448 |
| intracoronary adiponectin at reperfusion reduces infarct size in a porcine myocardial infarction model. | reperfusion injury (ri) remains an important limitation of myocardial revascularization. the aim of the present study was to evaluate the influence of the intracoronary injection of adiponectin on ri and cardiomyocyte death in a porcine myocardial infarction model. acute infarction in 14 polish domestic pigs was induced by inflation of an over the wire balloon (otw) catheter in the medial left anterior descending artery for 60-ámin. the study group consisted of 7 pigs in which intracoronary adip ... | 2011 | 21399860 |
| second prize: in-vivo physiological impact of alpha blockade on the porcine ureter with distal ureteral obstruction. | alpha blockade has been well described clinically for expulsion of distal ureteral stone. however, the in-vivo effects of a-blockade on ureteral dynamics during stone passage are not clear. we studied the effects of oral alfuzosin on ureteral pressure and peristalsis in a distally obstructed porcine ureter. | 2011 | 21401393 |
| vardenafil effect on ureteric smooth muscle: in vitro study in porcine model. | phosphodiesrase type 5 inhibitors have been recently reported to induce a relaxing effect on ureteral smooth muscle. we conducted an in vitro study to elucidate the relaxing effect of various doses of vardenafil on the porcine ureter. moreover, we propose a porcine ureter model for the evaluation of the effect of different substances on the ureter. | 2011 | 21401398 |
| age-related changes in corticosteroid receptor expression and monoamine neurotransmitter concentrations in various brain regions of postnatal pigs. | negative early life experience may be associated with altered functioning of stress-related systems and may increase vulnerability to diseases later in life. corticosteroids are important mediators of homeostasis and stress and exert their effects via two receptors, the mineralocorticoid receptor (mr) and the glucocorticoid receptor (gr), and through the glucocorticoid-metabolizing enzymes 11ß-hydroxysteroid dehydrogenase (11ß-hsd) types 1 and 2 in a brain-region-specific manner. however, relati ... | 2011 | 21416484 |
| the effect of combined delivery of recombinant human bone morphogenetic protein-2 and recombinant human vascular endothelial growth factor 165 from biomimetic calcium-phosphate-coated implants on osseointegration. | objectives: the delivery of growth factors for enhanced osseointegration depends on the effectiveness of the carrier systems at the bone-implant interface. this study evaluated the effect of solo and dual delivery of recombinant human bone morphogenetic protein-2 (rhbmp-2) and recombinant human vascular endothelial growth factor (rhvegf(165) ) from biomimetically octacalcium phosphate-coated implants on osseointegration. materials and methods: biomimetic implants, bearing either a single growth ... | 2011 | 21418332 |
| foot-and-mouth disease in feral swine: susceptibility and transmission. | experimental studies of foot-and-mouth disease (fmd) in feral swine are limited, and data for clinical manifestations and disease transmissibility are lacking. in this report, feral and domestic swine were experimentally infected with fmdv (a24-cruzeiro), and susceptibility and virus transmission were studied. feral swine were proved to be highly susceptible to a-24 cruzeiro fmd virus by intradermal inoculation and by contact with infected domestic and feral swine. typical clinical signs in fera ... | 2011 | 21418546 |
| welfare assessment in porcine biomedical research - suggestion for an operational tool. | in recent years, increasing interest in using the pig (sus scrofa) for biomedical research has become evident. today, the pig is considered an advantageous alternative animal model for various human diseases and conditions. however, even though a considerable amount of biomedical research has been done on pigs, hardly any studies include systematic welfare assessment. still, it is essential to assess welfare of laboratory pigs, both domestic pig breeds and smaller purpose-bred breeds, as (1) sci ... | 2011 | 21420697 |
| effects of local intracoronary paclitaxel delivery using the remedy transport catheter on neointimal hyperplasia after stent implantation in a porcine model. | to assess the effects of local paclitaxel delivery using the remedy catheter on neointimal hyperplasia in a porcine model and compare these results to commercially available bms and biodegradable polymer-coated paclitaxel-eluting stents (bp-pes). | 2011 | 21421185 |
| prevalence of classical swine fever virus in india: a 6-year study (2004-2010). | a study was undertaken regarding the prevalence of classical swine fever virus (csfv) antibodies and antigens in sera and suspected tissue samples of domestic pigs. the samples were received between january 2004 and september 2010. a total of 594 serum samples from 12 states and 287 tissue samples from 13 states of india were tested using commercial enzyme-linked immunosorbent assay (elisa) kits. the mean prevalence of csfv antibodies in suspected sera was 63.3% (376/594), whereas 76.7% (220/287 ... | 2011 | 21429182 |
| behavioural and cardiac responses towards conspecific distress calls in domestic pigs (sus scrofa). | in domestic pigs, vocalisation can be an indicator of distress and negative emotional states. it might play a role in the transfer of emotion between individuals ('emotional contagion' or 'empathy'), which could result in impaired animal welfare on a group level based on the distress in an individual member of the group. the aim of this study was to characterise the responses of pigs to conspecific distress calls. we performed a playback experiment in an open arena with 24 juvenile german landra ... | 2011 | 21439307 |
| comparative analysis of osseointegration of titanium implants with acid-etched surfaces and different biomolecular coatings. | objectives: an increasing trend toward implantation in complex cases, as well as early loading, is beginning to emerge in dental implantology. long-term stability of the inserted implants greatly depends on the osseointegration process. although there are numerous current research efforts aimed at functionalizing implant surfaces, no single factor has proved to be beneficial for osseointegration. the aim of the present study was to investigate whether a combination coating of collagen i and diff ... | 2011 | 21441047 |
| prevalence of antibody to toxoplasma gondii and trichinella spp. in feral pigs (sus scrofa) of eastern north carolina. | feral pigs (sus scrofa) survive in many climates, reproduce year-round, and are dietary generalists. in the united states, the size and range of the feral pig population has expanded, resulting in greater interaction with humans and domestic swine and increased potential for disease transmission. we conducted a serosurvey in feral pigs from eastern north carolina to determine exposure to the zoonotic parasites, toxoplasma gondii and trichinella spp. between september 2007 and march 2009, blood s ... | 2011 | 21441186 |
| semi-preparative isolation and purification of three tauro-conjugated cholic acids from pulvis fellis suis by hsccc coupled with elsd detection. | coupled with evaporative light scattering detection, a high-speed counter-current chromatography (hsccc) method was applied to the separation and purification of three tauro-conjugated cholic acids of taurochenodeoxycholic acid (tcdca), taurohyodeoxycholic acid (thdca) and taurohyocholic acid (thca) from pulvis fellis suis (pig gallbladder bile) for the first time. the two-phase solvent system composed of chloroform-methanol-water-acetic acid (4:4:2:0.3, v/v/v/v) was selected for the one-step se ... | 2011 | 21442029 |
| the diameter of anodic tio(2) nanotubes affects bone formation and correlates with the bone morphogenetic protein-2 expression in vivo. | introduction: recently, it has been demonstrated that the nanoscale environment is a critical factor for cellular behaviour. it has been shown that the diameter of tio(2) nanotube layers controls the cellular behaviour of cells involved in the bone-forming process in vitro. therefore, the aim of the present study was to investigate the effects of the diameter of tio(2) nanotubes on peri-implant bone formation and the expression of bone matrix proteins in vivo. materials and methods: ninety exper ... | 2011 | 21443609 |
| [standardized selection and its consequences for genetic population structure (examplified by sus scrofa l.)]. | type of selection for total fitness, specific for domestic animals and termed by us standardizing selection, is discussed. consequences of such selection for the population structure genetic are considered taking a population of domestic pigs as an example. examples of effective and ineffective standardizing selection are presented. specific features of genetic determination often quantitative traits detected in analysis of standardizing selection are discussed. | 2011 | 21446188 |
| combined chemical and optical methods for monitoring the early decay stages of surrogate human models. | as the body decays shortly after death, a variety of gases and volatile organic compounds (vocs) constantly emanate. ethical and practical reasons limit the use of human corpses in controlled, time-dependent, intervening experiments for monitoring the chemistry of body decay. therefore the utilization of pig carcasses serves as a potential surrogate to human models. the aim of this work was to study buried body decay in conditions of entrapment in collapsed buildings. six domestic pigs were used ... | 2011 | 21450424 |
| species-specific variation in rela underlies differences in nf-?b activity: a potential role in african swine fever pathogenesis. | african swine fever virus (asfv) is a highly infectious disease of domestic pigs, with virulent isolates causing a rapidly fatal hemorrhagic fever. in contrast, the porcine species endogenous to africa tolerate infection. the ability of the virus to persist in one host while killing another genetically related host implies that disease severity may be, in part, modulated by host genetic variation. to complement transcription profiling approaches to identify the underlying genetic variation in th ... | 2011 | 21450812 |
| quantitative computed tomography in porcine lung injury with variable versus conventional ventilation: recruitment and surfactant replacement. | biologically variable ventilation improves lung function in acute respiratory distress models. if enhanced recruitment is responsible for these results, then biologically variable ventilation might promote distribution of exogenous surfactant to nonaerated areas. our objectives were to confirm model predictions of enhanced recruitment with biologically variable ventilation using computed tomography and to determine whether surfactant replacement with biologically variable ventilation provides ad ... | 2011 | 21460711 |
| vaccination with viral protein-mimicking peptides postpones mortality in domestic pigs infected by african swine fever virus. | periodic outbreaks of african swine fever virus (asfv) infection around the world threaten local populations of domestic pigs with lethal disease and provide grounds for pandemic spread. effective vaccination may bring this threat under control. we investigated the effectiveness of select peptides mimicking viral proteins in establishing a protective immune response. forty-six synthetic peptides based on the analysis of the complete nucleotide sequence of asfv were tested for immunogenicity in m ... | 2011 | 21468582 |
| genetic diversity and positive selection analysis of classical swine fever virus isolates in south china. | classical swine fever virus (csfv) causes a highly contagious disease that leads to significant economic losses in the pig industry worldwide. however, there is a paucity of knowledge on the accurate genotyping of csfv isolates in south china. this study genotyped the e2 gene of 14 csfv strains isolated during 2008-2010 from domestic pigs in different districts of south china. phylogenetic analyses revealed that all of the 14 csfv isolates were clustered into genetic subgroup 1.1. this contrasts ... | 2011 | 21643769 |
| the first report of mycobacterium celatum isolation from domestic pig (sus scrofa domestica) and roe deer (capreolus capreolus) and an overview of human infections in slovenia. | mycobacterium celatum, a slowly growing potentially pathogenic mycobacterium first described in humans, is regarded as an uncommon cause of human infection, though capable of inducing invasive disease in immunocompromised hosts. according to some reports, a serious disease due to m. celatum may also occur in individuals with no apparent immunodeficiency. in animals, an m. celatum-related disease has been described in three cases only: twice in a domestic ferret (mustela putorius furo) and once i ... | 2011 | 21647336 |
| outbreak of swine erysipelas in a semi-intensive wild boar farm in spain. | swine erysipelas (se) is a disease caused by the bacterium erysipelothrix rhusiopathiae and is one of the best-known and most serious diseases affecting domestic pigs. however, few studies exist concerning the susceptibility of wild boars to this disease and the role of this species as a reservoir. this study investigates and describes an outbreak of se that occurred on a semi-intensive wild boar breeding farm housing 40 boars in extremadura (sw spain) on 11-18 february 2010. seven animals died, ... | 2011 | 21649879 |
| a pig model for the histomorphometric evaluation of hard tissue around dental implants. | abstract this study aimed to evaluate the frontal bone of swiss domestic pigs as an animal model for the histologic-histomorphometric examination of bony tissue around dental implants. sla surface implants 4.1 mm in diameter and 10 mm in length were inserted into the frontal bones of 9 swiss-domestic pigs. histologic and histomorphometric studies were conducted on the undecalcified sections. histological examinations showed that the specimens contain sufficient amount of bone, which provides hom ... | 2011 | 21651415 |
| middle ear of human and pig: a comparison of structures and mechanics. | animal studies are an essential method to evaluate implants for the middle ear prior to clinical studies. new zealand rabbits, guinea pigs and chinchillas are, among other small mammals, well established animal models, but their auditory system is significantly smaller compared to human. hence, the suitability of the domestic pig (sus scrofa domesticus) as a new animal model for research on the middle ear (me), that would match the human in size, was investigated. | 2011 | 21657989 |
| quality traits of wild boar mould-ripened salami manufactured with different selections of meat and fat tissue, and with and without bacterial starter cultures. | quality traits of wild boar mould-ripened salami were assessed in eight batches produced from two different assortments of meat (hind leg vs. shoulder), fat tissue (backfat from domestic pigs vs. fat tissue from wild boar), and with and without addition of a commercial bacterial starter culture. chemical composition of finished products (day 35) were in compliance with national food codex. batches produced with a bacterial starter culture were generally preferred by taste panels, had significant ... | 2011 | 21664055 |
| heart-lung interactions measured by electrical impedance tomography. | : the clinical value of stroke volume variations to assess intravascular fluid status in critically ill patients is well known. electrical impedance tomography is a noninvasive monitoring technology that has been primarily used to assess ventilation. we investigated the potential of electrical impedance tomography to measure left ventricular stroke volume variation as an expression of heart-lung interactions. the objective of this study was thus to determine in a set of different hemodynamic con ... | 2011 | 21666450 |
| transmission of salmonella between swine farms by the housefly (musca domestica). | the domestic pig is an important source of human salmonellosis, and houseflies are potential mechanical vectors of foodborne salmonella pathogens. in 2005, we recovered 144 salmonella isolates from flies and swine stool samples from 11 farms in taoyuan county and hsin chu county (northwestern taiwan). a total of 71.5% of the isolates were resistant to at least three antibiotics. there were a total of 14 serotypes, and 8 of these serotypes were present in both flies and swine stool samples. some ... | 2011 | 21669083 |
| measurement of the midpalatal suture width. | abstract objective: to conduct a pilot study to investigate the potentiality to determine the midpalatal sutural width radiographically with a flat-panel volume computed tomography (fpvct) in a porcine model. materials and methods: bone samples from the midpalatal suture of five young (16 weeks) and five old (200 weeks) sus scrofa domestica were gathered. the midpalatal suture width was measured via fpvct and compared to respective histological preparations. results with p < .05 were considered ... | 2011 | 21812573 |
| systemic gene delivery in large species for targeting spinal cord, brain, and peripheral tissues for pediatric disorders. | adeno-associated virus type 9 (aav9) is a powerful tool for delivering genes throughout the central nervous system (cns) following intravenous injection. preclinical results in pediatric models of spinal muscular atrophy (sma) and lysosomal storage disorders provide a compelling case for advancing aav9 to the clinic. an important translational step is to demonstrate efficient cns targeting in large animals at various ages. in the present study, we tested systemically injected aav9 in cynomolgus ... | 2011 | 21811247 |
| hepatitis e virus in domestic pigs and surface waters in slovenia: prevalence and molecular characterization of a novel genotype 3 lineage. | a novel hepatitis e virus (hev) genotype 3 lineage was identified in slovenian pig herds. stool samples from six slovenian pig farms were collected and tested for the presence of hev rna. of 85 individual samples 15 (20.3%) were positive for hev rna of which 2/38 (5.3%), 6/21 (28.6%) and 7/26 (26.9%) were from suckling, weanling and fattening pigs, respectively. additionally, 51 pooled porcine stool samples were tested in one of the biggest pig farm and the estimated infection rate of individual ... | 2011 | 21802527 |
| the codon usage model of the context flanking each cleavage site in the polyprotein of foot-and-mouth disease virus. | to investigate the codon usage pattern of the contexts flanking 11 cleavage sites of foot-and-mouth disease virus (fmdv) polyprotein, the codon usage model of the corresponding codon position and the synonymous codon usage in the target contexts of 66 strains were characterized by two simple methods based on the relative synonymous codon usage value. the synonymous codons usage pattern was also compared between this virus and two species of hosts (cattle and domestic pig). it is indicated that f ... | 2011 | 21801856 |
| african swine fever virus p72 genotype ix in domestic pigs, congo, 2009. | african swine fever virus p72 genotype ix, associated with outbreaks in eastern africa, is cocirculating in the republic of the congo with west african genotype i. data suggest that viruses from eastern africa are moving into western africa, increasing the threat of outbreaks caused by novel viruses in this region. | 2011 | 21801650 |
| connexin 43 gene therapy prevents persistent atrial fibrillation in a porcine model. | aims atrial fibrillation (af) is the most common sustained cardiac arrhythmia, and effective treatment of af still remains an unmet medical need. af is associated with atrial conduction disturbances caused by electrical and/or structural remodeling. we hypothesized that af suppresses expression of the gap junction protein connexin 43, and that cx43 gene transfer to both atria would prevent persistent atrial fibrillation. the first aim of this study was to assess whether atrial fibrillation (af) ... | 2011 | 21799069 |
| catheter-based renal sympathetic denervation: chronic preclinical evidence for renal artery safety. | background: renal sympathetic hyperactivity is associated with hypertension, a leading cause of mortality worldwide. renal sympathetic denervation via the symplicity catheter system has been shown to decrease blood pressure by 33/11 mmhg by 6 months, with no radiofrequency (rf)-related adverse sequelae visible by ct/mr angiography or renal duplex ultrasound 6 months after the procedure. here, we present preclinical work predating those clinical results. we performed therapeutic renal sympathetic ... | 2011 | 21796327 |
| comparison of anterior transgastric access techniques for natural orifice translumenal endoscopic surgery. | background: the advancement of natural orifice translumenal endoscopic surgery (notes) into clinical practice is dependent on its safety, efficacy, and efficiency. access is the obligatory first step in notes and serves as a surrogate to technical difficulties associated with this novel surgical approach. this study aimed to compare endoscopic transgastric access techniques in terms of safety, reproducibility, and efficiency. methods: seven variations for anterior transgastric notes access were ... | 2011 | 21789648 |
| porcine kobuvirus from pig stool specimens in shanghai, china. | in this study, a total of 116 stool specimens were collected from pigs of different ages in three pig farms of shanghai in china during 2010. forty-five (38.8%) stool specimens were positive for porcine kobuvirus using reverse transcriptase-polymerase chain reaction (rt-pcr). the prevalence rate of porcine kobuvirus in the three pig farms which are located in minhang district, qingpu district and fengxian district was 46.7%(21/45), 35.1%(13/37), and 32.4%(11/34), respectively. we demonstrated th ... | 2011 | 21789634 |
| influence of tongue inspection during cysticercosis diagnosis on some behavioral and physiological stress measures in pigs (sus scrofa domestica). | to evaluate the level of stress induced by the tongue inspection method during cysticercosis diagnosis in pigs at 60 and 90 kg, 27 animals were assigned to one of three experimental groups. in the first group (t1), pigs were held with a snout noose. in addition, animals in t2 were subjected to tongue inspection, while in t0, none of the procedures described above were performed. resistance to be handled and serum cortisol concentration were higher (p?<?0.05) than those from t0 in t1 and t2 group ... | 2011 | 21786048 |
| suppression of persistent atrial fibrillation by genetic knockdown of caspase 3: a pre-clinical pilot study. | aims atrial fibrillation (af) is linked to cardiomyocyte apoptosis, leading to atrial remodelling and reduction in electrical conduction velocity. we hypothesized that genetic suppression of an apoptotic key enzyme, caspase 3, would prevent the development of persistent af by reducing apoptosis which may serve as an arrhythmogenic substrate. methods and results atrial fibrillation was induced in domestic pigs by atrial burst pacing via an implanted cardiac pacemaker. study animals were then assi ... | 2011 | 21785105 |
| vertebral growth modulation by hemicircumferential electrocoagulation: an experimental study in pigs. | introduction: this experimental study in pigs was aimed at evaluating spinal growth disorders after partial arrest of the vertebral epiphyseal plates (ep) and neurocentral cartilages (ncc). unilateral and multisegmental single or combined lesions of the physeal structures were performed by electrocogulation throughout a video-assisted thoracoscopical approach. materials and methods: thirty 4-week-old domestic pigs (mean weight 16 kg) were included in the experiments. the superior and inferior ep ... | 2011 | 21779856 |
| cardiac aav9-s100a1 gene therapy rescues post-ischemic heart failure in a preclinical large animal model. | as a prerequisite for clinical application, we determined the long-term therapeutic effectiveness and safety of adeno-associated virus (aav)-s100a1 gene therapy in a preclinical large animal model of heart failure. s100a1, a positive inotropic regulator of myocardial contractility, becomes depleted in failing cardiomyocytes in humans and animals, and myocardial-targeted s100a1 gene transfer rescues cardiac contractile function by restoring sarcoplasmic reticulum calcium (ca(2+)) handling in acut ... | 2011 | 21775667 |
| mri studies of cryoinjury infarction in pig hearts: ii. effects of intrapericardial delivery of adipose-derived stem cells (adsc) embedded in agarose gel. | the purpose was to assess effects of intrapericardially deposited adipose-derived stem cells (adsc) as a source of angiogenic factors on cryoinjury infarction. to enhance this effect and reduce incorporation of adsc into tissue, the cells were immobilized in agarose gel patches transplanted onto cryoinjured epicardium. in domestic pigs (15-20?kg) the left ventricular (lv) anterior wall of exposed hearts was cryoinjured using aluminum rod (f=25?mm) cooled in liquid nitrogen. sterilized circular p ... | 2011 | 21774011 |
| prevalence of hepatitis e virus-specific antibodies in humans with occupational exposure to pigs. | due to the increasing number of non-travel-associated hepatitis e virus (hev) infections observed in several industrialised countries including germany, there is a substantial interest in the characterisation of risk factors and transmission routes relevant to autochthonous hev infections. autochthonous cases are believed to be the result of a zoonotic hev transmission from pigs, wild boars and deer. recently, a high prevalence of hev-specific antibodies in the german domestic pig population has ... | 2011 | 21773797 |
| molecular identification and phylogenetic analysis of trichinella isolates from different provinces in mainland china. | polymerase chain reaction (pcr) and sequencing are useful for species identification of trichinella spp., especially when their morphological characteristics useful for identifying taxa are lacking. in the present study, nine trichinella isolates from different provinces in mainland china were identified by the pcr-based method using the 5s ribosomal dna intergene spacer region (5s isr) and the mitochondrial large subunit ribosomal dna genes as molecular markers. the results indicated that eight ... | 2011 | 21773771 |
| mitochondrial targeting of peroxiredoxin 5 is preserved from annelids to mammals but is absent in pig sus scrofa domesticus. | peroxiredoxin 5 (prdx5) is a thioredoxin peroxidase able to reduce hydrogen peroxide, alkyl hydroperoxides and peroxynitrite. in human, prdx5 was reported to be localized in the cytosol, the mitochondria, the peroxisomes and the nucleus. mitochondrial localization results from the presence of an n-terminal mitochondrial targeting sequence (mts). here, we examined the conservation of mitochondrial localization of prdx5 in animal species. we found that prdx5 mts is present and functional in the an ... | 2011 | 21763786 |
| mesenchymal stem cells show radioresistance in vivo. | irradiation impacts on the viability and differentiation capacity of tissue-borne mesenchymal stem cells (msc), which play a pivotal role in bone regeneration. as a consequence of radiotherapy, bones may develop osteoradionecrosis. when irradiating human bone-derived msc in vitro with increasing doses, the cells' self-renewal capabilities were greatly reduced. mitotically stalled cells were still capable of differentiating into osteoblasts and preadipocytes. as a large animal model comparable to ... | 2011 | 21762375 |
| determination of the mechanical properties of the skin of pig foetuses with respect to its structure. | skin is an important barrier protecting the organism against external environmental factors. determination of its mechanical characteristics as regards its structure has significant scientific and application value. in this work, uniaxial tensile tests were conducted to determine the basic mechanical parameters of skin with respect to its structure. the subject of the study were skin samples taken from domestic pig foetuses. they were excised from different parts of body, in the direction parall ... | 2011 | 21761809 |
| sildenafil prevents the increase of extravascular lung water and pulmonary hypertension after meconium aspiration in newborn piglets. | 2011 | 21755265 | |
| mrna expression profiles of p450 3a enzymes in the liver and small intestine of the domestic pig. | the cytochrome p450 (p450) 3a family is considered to be the most important and abundantly expressed p450 subfamily in mammals. the mrna expression levels of four p450 3a enzymes in porcine liver and small intestine were investigated using real-time reverse transcriptase-polymerase chain reaction (rt-pcr). the expression of p450 3a mrnas (p450 3a pool) was higher in the liver than that in the small intestine. in the small intestine, the p450 3a mrnas were gradually decreased from the duodenum to ... | 2011 | 21752411 |
| diabetes mellitus negatively affects peri-implant bone formation in the diabetic domestic pig. | von wilmowsky c, stockmann p, harsch i, amann k, metzler p, lutz r, moest t, neukam fw, schlegel ka: diabetes mellitus negatively affects peri-implant bone formation in the diabetic domestic pig. j clin periodontol 2011; 38: 771-779. doi: 10.1111/j.1600-051x.2011.01746.x. abstract: aim: diabetes mellitus is classified as a relative contraindication for implant treatment, and higher failure rates have been seen in diabetic patients. the aim of the present study was to investigate the effect of di ... | 2011 | 21752046 |
| feasibility of initiating extracorporeal life support during mechanical chest compression cpr: a porcine pilot study. | background: recently, portable extracorporeal membrane oxygenation (ecmo) machines have become commercially available. this creates the potential to utilize extracorporeal life support (ecls) for the treatment of sudden cardiac arrest in the emergency department, and potentially in the out-of-hospital setting. objective: we sought to determine the feasibility of installing the ecmo circuit during delivery of mechanical chest compression cpr. methods: we used 5 mixed-breed domestic swine with a m ... | 2011 | 21835144 |
| epidemiological and clinical aspects of human brucella suis infection in polynesia. | summaryhigh brucellosis seroprevalence rates in domestic swine herds have been reported in wallis and futuna islands and are associated with a significant burden of human infection by brucella suis, a species that is rarely incriminated in human disease. between 2003 and 2010, seven patients had a positive blood culture for b. suis biovar 1, 11 symptomatic patients had a positive rose bengal test (rbt) and a positive serum agglutination test (sat) and three asymptomatic cases were found to be po ... | 2011 | 21733252 |
| sequencing technologies and genome sequencing. | the high-throughput - next generation sequencing (ht-ngs) technologies are currently the hottest topic in the field of human and animals genomics researches, which can produce over 100 times more data compared to the most sophisticated capillary sequencers based on the sanger method. with the ongoing developments of high throughput sequencing machines and advancement of modern bioinformatics tools at unprecedented pace, the target goal of sequencing individual genomes of living organism at a cos ... | 2011 | 21698376 |
| sensitivity and ex vivo validation of finite element models of the domestic pig cranium. | a finite element (fe) validation and sensitivity study was undertaken on a modern domestic pig cranium. bone strain data were collected ex vivo from strain gauges, and compared with results from specimen-specific fe models. an isotropic, homogeneous model was created, then input parameters were altered to investigate model sensitivity. heterogeneous, isotropic models investigated the effects of a constant-thickness, stiffer outer layer (representing cortical bone) atop a more compliant interior ... | 2011 | 21718316 |
| seroprevalence of taenia solium infections in croatian patients presenting with epilepsy. | epilepsy is one of the most common neurological disorders, while neurocysticercosis caused by taenia solium infection of the central nervous system currently represents the leading cause of secondary epilepsy in central and south america, east and south asia, and sub-saharan africa. as a result of increased migration from these endemic regions, neurocysticercosis and subsequent epilepsy are becoming a growing public health problem in developed countries as well. in order to determine the prevale ... | 2011 | 21729385 |
| pluripotent stem cells and reprogrammed cells in farm animals. | pluripotent cells are unique because of their ability to differentiate into the cell lineages forming the entire organism. true pluripotent stem cells with germ line contribution have been reported for mice and rats. human pluripotent cells share numerous features of pluripotentiality, but confirmation of their in vivo capacity for germ line contribution is impossible due to ethical and legal restrictions. progress toward derivation of embryonic stem cells from domestic species has been made, bu ... | 2011 | 21682936 |
| genetic characterization and phylogeography of the wild boar sus scrofa introduced into uruguay. | the european wild boar sus scrofa was first introduced into uruguay, in southern south america during the early decades of the last century. subsequently, and starting from founder populations, its range spread throughout the country and into the neighbouring brazilian state rio grande do sul. due to the subsequent negative impact, it was officially declared a national pest. the main aim in the present study was to provide a more comprehensive scenario of wild boar differentiation in uruguay, by ... | 2011 | 21734838 |
| impact of epididymal maturation, ejaculation and in vitro capacitation on tyrosine phosphorylation patterns exhibited of boar (sus domesticus) spermatozoa. | mammalian spermatozoa acquire functionality during epididymal maturation and ability to penetrate and fertilize the oocyte during capacitation. the aim of this study was to investigate the impact of epididymal maturation, ejaculation and capacitation on phosphotyrosine content of sperm proteins. western blot, immunocytochemical and flow cytometry analyses demonstrated that epididymal maturation in vivo is associated with a progressive loss of phosphotyrosine residues of the sperm head followed b ... | 2011 | 21835448 |
| hemodynamic and histologic characterization of a swine (sus scrofa domestica) model of chronic pulmonary arterial hypertension. | the purpose of this work was to develop and characterize an aortopulmonary shunt model of chronic pulmonary hypertension in swine and provide sequential hemodynamic, angiographic, and histologic data by using an experimental endoarterial biopsy catheter. nine yucatan female microswine (sus scrofa domestica) underwent surgical anastomosis of the left pulmonary artery to the descending aorta. sequential hemodynamic, angiographic, and pulmonary vascular samples were obtained. six pigs (mean weight, ... | 2011 | 21819696 |
| gouleako virus isolated from west african mosquitoes constitutes a proposed novel genus in the family bunyaviridae. | the family bunyaviridae is the most diversified family of rna viruses. we describe a novel prototypic bunyavirus, tentatively named goul+®ako virus, isolated from various mosquito species trapped in c+¦te d'ivoire. the s segment comprised 1,087 nucleotides (nt), the m segment 3,188 nt, and the l segment 6,358 nt, constituting the shortest bunyavirus genome known so far. the virus had shorter genome termini than phleboviruses and showed no evidence of encoded nss and nsm proteins. an uncharacteri ... | 2011 | 21715500 |
| pathologic evaluation of hemostatic agents in percutaneous nephrolithotomy tracts in a porcine model. | abstract background and purpose: hemostatic agents have been suggested as an adjunct for tubeless percutaneous nephrolithotomy (pcnl). we pathologically evaluated the percutaneous tracts injected with the fibrin sealant (fs) evicel and hemostatic gelatin matrix (hgm) surgiflo at various time intervals to determine their absorption and tract closure rates. we also evaluated whether these agents reduced urine leak rates in a porcine model. materials and methods: percutaneous access was obtained in ... | 2011 | 21740197 |
| genome engineering with zinc-finger nucleases. | zinc-finger nucleases (zfns) are targetable dna cleavage reagents that have been adopted as gene-targeting tools. zfn-induced double-strand breaks are subject to cellular dna repair processes that lead to both targeted mutagenesis and targeted gene replacement at remarkably high frequencies. this article briefly reviews the history of zfn development and summarizes applications that have been made to genome editing in many different organisms and situations. considerable progress has been made i ... | 2011 | 21828278 |
| current perspectives on the phylogeny of filoviridae. | sporadic fatal outbreaks of disease in humans and non-human primates caused by ebola or marburg viruses have driven research into the characterization of these viruses with the hopes of identifying host tropisms and potential reservoirs. such an understanding of the relatedness of newly discovered filoviruses may help to predict risk factors for outbreaks of hemorrhagic disease in humans and/or non-human primates. recent discoveries such as three distinct genotypes of reston ebolavirus, unexpect ... | 2011 | 21742058 |
| selection of reference genes for gene expression studies in pbmc from bama miniature pig under heat stress. | heat stress decreases immune function and increases disease susceptibility in stressed animals, which are important factors for industry and public health. we investigated the molecular mechanisms underlying heat stress by profiling the expression of target genes involved in the cellular response in the blood of bama miniature pigs (sus scrofa domestica) over 21 days with the use of quantitative rt-pcr (qrt-pcr). reliable standards were established for the normalization of qrt-pcr. six potential ... | 2011 | 21820186 |
| toxoplasma gondii in switzerland: a serosurvey based on meat juice analysis of slaughtered pigs, wild boar, sheep and cattle. | toxoplasmosis is one of the most important zoonotic diseases worldwide and is caused by the protozoan toxoplasma gondii. besides vertical infection during pregnancy, humans can get infected post-natally either by peroral uptake of sporulated toxoplasma oocysts or by ingestion of tissue cysts upon consumption of raw or undercooked meat. the aim of this study was to approximate the risk of human infection via meat consumption by estimating the seroprevalence of t. gondii in slaughtered animals in ... | 2011 | 21824348 |
| enhanced delivery of topically-applied formulations following skin pre-treatment with a hand-applied, plastic microneedle array. | the purpose of this work is to characterize microchannels created by polymeric microneedles, applied by hand, and to demonstrate enhanced delivery of topically applied formulations of lidocaine hydrochloride and methylprednisolone sodium succinate (mpss). 3m's microstructured transdermal system (mts) arrays were applied to domestic swine to demonstrate reliability of penetration, depth of penetration and durability of the structures to repeat application and high force. tissue levels of lidocain ... | 2011 | 21696356 |
| randomized objective comparison of live tissue training versus simulators for emergency procedures. | there is a lack of objective analysis comparing live tissue and simulator training. this article aims to objectively determine the difference in outcomes. twenty-four air force volunteers without prior experience performing emergency procedures were randomly assigned to receive training in tube thoracostomy (chest tube) and cricothyroidotomy training on either a pig model (sus scrofa domestica) or on the traumaman simulator. one week posttraining, students were tested on human cadavers with obje ... | 2011 | 21679588 |
| neisseria meningitidis and escherichia coli are protected from leukocyte phagocytosis by binding to erythrocyte complement receptor 1 in human blood. | the initial interaction of gram-negative bacteria with erythrocytes and its implications on leukocyte phagocytosis and oxidative burst in human whole blood were examined. alexa-labeled escherichia coli, wild-type h44/76 n. meningitidis and the h44/76lpxa lipopolysaccharide (lps)-deficient mutant were incubated with whole blood using lepirudin as anticoagulant which has no adverse effects on complement. bacteria free in plasma, bound to erythrocytes or phagocytized by granulocytes and monocytes w ... | 2011 | 21839519 |
| genetic detection and analysis of porcine bocavirus type 1 (pobov1) in european wild boar (sus scrofa). | novel porcine parvoviruses showing the genetic characteristics of bocaviruses have recently been identified. the first such porcine bocavirus (pobov1), described as boca-like virus (pbo-likev), was discovered in pmws affected pigs in sweden. later, several other bocaviruses with divergent genomes were reported under various names in domestic pigs. this is the first report of the presence of bocaviruses in european wild boars. 842 wild boar samples originating from the western region of romania ( ... | 2011 | 21822671 |
| protective effect of nac preconditioning against ischemia-reperfusion injury in piglet small bowel transplantation: effects on plasma tnf, il-8, hyaluronic acid, and no. | ischemia-reperfusion (i/r) injury is one of the main factors affecting the function and structure of small bowel transplantation (sbt), by generation of proinflammatory mediators such as reactive oxygen species, reactive nitrogen species, cytokines, and endotoxin. experimental data have demonstrated that n-acetylcysteine (nac) attenuates intestinal i/r injury. the objective of this study was to determine the effect of nac preconditioning on the sbt-i/r induced inflammatory cascade, with particul ... | 2011 | 20036383 |
| mitochondrial dna diversity in wild boar from the primorsky krai region (east russia). | we have studied the cytochrome b gene and control region dna variability in 14 wild boars from the primorsky region, in the far east corner of russia. variability was low (π = 0.00248 overall) compared with the usual estimates in these loci, indicating that this is a rather closed population. seven haplotypes were found, and one was identical to a chinese wild boar. phylogeographically, the sequences clustered among several asian clades, primarily chinese domestic pigs and japanese and chinese w ... | 2011 | 20477807 |
| divergence time estimates of east asian and european pigs based on multiple near complete mitochondrial dna sequences. | the time since the divergence of european and east asian domestic pigs and wild boars has been estimated in several phylogenetic analyses, generally based on partial mitochondrial sequences or on a small number of complete mtdna sequences. in the present study, we obtained a refined estimate of this divergence time based on a set of 32 near-complete mtdna sequences from wild and domestic pigs of european and asian types, including 14 new and 18 previously published sequences. a weighted average ... | 2011 | 20477794 |
| cutaneous challenge with chemical warfare agents in the skh-1 hairless mouse (ii): effects of some currently used skin decontaminants (rsdl and fuller's earth) against liquid sulphur mustard and vx exposure. | using the hairless mouse screening model presented in the companion paper(1) the aim of this study was to assess two skin decontaminating systems: fuller's earth (fe) and reactive skin decontamination lotion (rsdl) against two extremely toxic chemical warfare agents that represent a special percutaneous hazard, sulphur mustard (sm) and o-ethyl-s-(2[di-isopropylamino]ethyl)methyl-phosphonothioate (vx). five minutes after being exposed on the back to either 2 µl of neat sulphur mustard or 50 µg.kg ... | 2011 | 20534641 |
| 1.5:1 meshed alloderm bolsters for stapled rectal anastomoses does not provide any advantage in anastomotic strength in a porcine model. | the most feared complication of colorectal anastomoses is leaks resulting in severe morbidity. the concept of staple-line reinforcement is a growing area of interest. in this study, the authors evaluated the feasibility and effect of using 1.5:1 meshed alloderm to bolster end-to-end stapled rectal anastomoses in a porcine model. | 2011 | 20542954 |
| rapid intradermal delivery of liquid formulations using a hollow microstructured array. | the purpose of this work is to demonstrate rapid intradermal delivery of up to 1.5 ml of formulation using a hollow microneedle delivery device designed for self-application. | 2011 | 20582455 |
| effects of sodium selenite and amiloride on calvarial calcification in closing small cranial defects. | fresh autogenous bone graft is the most preferred osteoplastic material, whether the purpose is cosmetic, psychological, or for the protection of the brain. these grafts are not rejected and do not react immunologically. the aim of this study was to evaluate the efficacy of autogenous fat rolled with bone dust derived from the bur hole in closing small cranial defects. additionally, the authors examined the morphological and biochemical effects of na selenite and amiloride on calvarial calcifica ... | 2011 | 20672896 |
| durability of the self-approximating translumenal access technique (stat) for potential use in natural orifice translumenal surgery (notes). | the self-approximating translumenal access technique (stat) has been shown to provide a safe and reliable means of abdominal access for natural orifice translumenal endoscopic surgery (notes). however, the feasibility of using stat for translumenal organ resection is unknown. this study aimed to evaluate the technical performance of organ resection using stat, the integrity of the stat gastric tunnel after organ resection, and the postoperative morbidity of organ resection using stat. | 2011 | 20725749 |
| peptide vaccine candidates against classical swine fever virus: t cell and neutralizing antibody responses of dendrimers displaying e2 and ns2-3 epitopes. | three peptide-based systems integrating b and t antigenic sites of csfv and displaying the b epitopes in fourfold presentation have been designed and produced, and shown to bring about significant enhancements in immunogenicity over the peptides in monomeric form. of the different strategies tested for producing the dendrimeric constructs, stepwise spps using 3,6-dioxaoctanoic acid as flexible, peg-like spacer units at the branching points is clearly advantageous, in particular over ligation in ... | 2011 | 20812373 |
| new piophilid in town: the first palaearctic record of piophila megastigmata and its coexistence with piophila casei in central spain. | the genus piophila fallén (diptera: piophilidae) is known from only two species: piophila casei (l.) is a major pest, a cosmopolitan species and is commonly used as a forensic indicator, whereas piophila megastigmata mcalpine has until now only been recorded in a natural environment in south africa. the present work reports the first occurrence of p. megastigmata in the palaearctic region from specimens collected by carrion-baited traps throughout different natural habitats of central spain. fur ... | 2011 | 20819151 |
| video: transcervical videoscopic esophageal dissection in minimally invasive esophagectomy. | minimally invasive esophagectomy (mie) may involve video-assisted thoracoscopic surgery (vats) for mediastinal esophageal dissection. usually, vats requires single-lung ventilation and has associated cardiopulmonary morbidity [1-3]. alternatively, transhiatal dissection can be performed, although its complications include vocal cord palsy [4], cardiac arrythmias [5], and increased bleeding [5, 6], the latter associated with mortality after esophagectomy [2]. therefore, the feasibility of mie usi ... | 2011 | 20844900 |
| comparison of the substrate kinetics of pig cyp3a29 with pig liver microsomes and human cyp3a4. | cyp (cytochrome p450) 3a29 in pigs could be an important candidate gene responsible for xenobiotic metabolism, similar to cyp3a4 in humans. accordingly, the tissue expression of cyp3a29 mrna in domestic pigs has been determined by a real-time pcr. the enzymatic properties of cyp3a29, cyp3a4 and plm (pig liver microsomes) were compared by kinetic analysis of tst (testosterone) 6β-hydroxylation and nif (nifedipine) oxidation. cyp3a29 mrna was highly expressed in the liver and small intestines of d ... | 2011 | 20863320 |
| a preclinical in vivo investigation of high-intensity focused ultrasound combined with radiotherapy. | this study aims to perform an in vivo investigation evaluating the injury to the pancreas and adjacent tissue of swine resulting with high-intensity focused ultrasound (hifu) combined with radiotherapy (rt). the protocol was approved by the animal ethics committee at the peking university first hospital. a total of 12 domestic swine were divided into four groups: control, hifu only, rt only and hifu + rt. the injury to the pancreas, adjacent tissue and tissue within the acoustic path of the hifu ... | 2011 | 21144959 |
| identification of low and high frequency ranges for heart rate variability and blood pressure variability analyses using pharmacological autonomic blockade with atropine and propranolol in swine. | understanding autonomic nervous system functioning, which mediates behavioral and physiological responses to stress, offers great potential for assessing farm animal stress and welfare. evaluation of heart rate variability (hrv) and blood pressure variability (bpv), using time and frequency domain analyses may provide a sensitive and reliable measure of affective states and stress-mediated changes in sympathetic and parasympathetic tones. the aim of this research was to define low (lf) and high ... | 2011 | 21281655 |
| molecular mechanism by which amp-activated protein kinase activation promotes glycogen accumulation in muscle. | during energy stress, amp-activated protein kinase (ampk) promotes glucose transport and glycolysis for atp production, while it is thought to inhibit anabolic glycogen synthesis by suppressing the activity of glycogen synthase (gs) to maintain the energy balance in muscle. paradoxically, chronic activation of ampk causes an increase in glycogen accumulation in skeletal and cardiac muscles, which in some cases is associated with cardiac dysfunction. the aim of this study was to elucidate the mol ... | 2011 | 21282366 |
| coronary vein infusion of multipotent stromal cells from bone marrow preserves cardiac function in swine ischemic cardiomyopathy via enhanced neovascularization. | few reports have examined the effects of adult bone marrow multipotent stromal cells (mscs) on large animals, and no useful method has been established for msc implantation. in this study, we investigate the effects of msc infusion from the coronary vein in a swine model of chronic myocardial infarction (mi). mi was induced in domestic swine by placing beads in the left coronary artery. bone marrow cells were aspirated and then cultured to isolate the mscs. at 4 weeks after mi, mscs labeled with ... | 2011 | 21283079 |
| discovery of porcine micrornas in multiple tissues by a solexa deep sequencing approach. | the domestic pig (sus scrofa) is an important economic animal for meat production and as a suitable model organism for comparative genomics and biomedical studies. in an effort to gain further identification of mirnas in the pig, we have applied the illumina solexa sequencing technology to carry out an in-depth analysis of the mirna transcriptome in a pool of equal amounts of rna from 16 different porcine tissues. from this data set, we identified 437 conserved and 86 candidate novel mirna/mirna ... | 2011 | 21283541 |
| the parameters of the porcine eyeball. | the eye of the domestic pig (sus scrofa domestica) is an ex vivo animal model often used in vision sciences research (retina studies, glaucoma, cataracts, etc.). however, only a few papers have compiled pig eye anatomical descriptions. the purpose of this paper is to describe pig and human eye anatomical parameters to help investigators in their choice of animal model depending on their study objective. | 2011 | 21287191 |
| different enteropathogenic yersinia strains found in wild boars and domestic pigs. | yersinia enterocolitica and yersinia pseudotuberculosis strains isolated from wild boars and fattening pigs were characterized and compared with each other. in wild boars, ail-positive y. enterocolitica strains belonged to bioserotypes 4/o:3 (36%, 5/14), 2/o:9 (29%, 4/14), and 2/o:5,27 (21%, 3/14). additionally, two ail-positive strains were untypable. among fattening pigs, the bioserotype 4/o:3 was dominating (91%, 71/78), and bioserotypes 2/o:5,27 (8%, 6/78) and 2/o:9 (1%, 1/78) were rare. inv ... | 2011 | 21288132 |
| trpml2 and the evolution of mucolipins. | trpml2, the polypeptide product of the gene trpml2 (aka mcoln2), is a member of the trpml or mucolipin branch of the trp super family of ion channels. although no known agonists have been discovered, the wild type channel gives basal currents when heterologously expressed in drosophila (s2) cells and is constitutively active in mammalian cells when bearing a cell degeneration-causing, proline to alanine substitution in the fifth trans-membrane domain. trpml2 forms channels that are inwardly rect ... | 2011 | 21290298 |
| changes of dna methylation level and spatial arrangement of primordial germ cells in embryonic day 15 to embryonic day 28 pig embryos. | the mammalian germline is generally assumed to undergo extensive epigenetic reprogramming during embryonic development, including a nearly complete erasure of dna methylation. this assumption does, however, to large degree rely on data from mouse, and despite a well-grounded picture the general nature of these data needs to be validated by investigations of other mammalian species. this study represents such a contribution in the examination of the germline in the domestic pig (sus scrofa). semi ... | 2011 | 21293033 |
| the local origin of the tibetan pig and additional insights into the origin of asian pigs. | the domestic pig currently indigenous to the tibetan highlands is supposed to have been introduced during a continuous period of colonization by the ancestors of modern tibetans. however, there is no direct genetic evidence of either the local origin or exotic migration of the tibetan pig. | 2011 | 22163285 |
| medicinal animals used in ethnoveterinary practices of the 'cariri paraibano', ne brazil. | zootherapy is important in various socio-cultural environments, and innumerous examples of the use of animal derived remedies can currently be found in many urban, semi-urban and more remote localities in all parts of the world, particularly in developing countries. however, although a number of ethnobiological inventories concerning the use of medicinal animals in human health care have been compiled in brazil in recent years, zootherapeutic practices in ethnoveterinary medicine (evm) are poorl ... | 2011 | 21985401 |
| extensive polymorphism in the porcine toll-like receptor 10 gene. | the great importance of the toll-like receptors (tlrs) in innate immunity is well established, but one family member - tlr10 - remains elusive. tlr10 is expressed in various tissues in several species, but its ligand is not known and its function is still poorly understood. the open reading frame of tlr10 was sequenced in 15 wild boars, representing three populations, and in 15 unrelated domestic pigs of hampshire, landrace and large white origin. amino acid positions corresponding to detected n ... | 2011 | 22077964 |
| the pig: a model for human infectious diseases. | an animal model to study human infectious diseases should accurately reproduce the various aspects of disease. domestic pigs (sus scrofa domesticus) are closely related to humans in terms of anatomy, genetics and physiology, and represent an excellent animal model to study various microbial infectious diseases. indeed, experiments in pigs are much more likely to be predictive of therapeutic treatments in humans than experiments in rodents. in this review, we highlight the numerous advantages of ... | 2011 | 22153753 |
| preserved heart rate variability during therapeutic hypothermia correlated to 96 hours neurological outcomes and survival in a pig model of cardiac arrest. | objective:: therapeutic hypothermia initiated with cardiopulmonary resuscitation improves neurologic outcomes and survival after prolonged cardiac arrest. however, the potential mechanism by which hypothermia improves neurologic outcomes remains unclear. in the current study, we investigated the effect of rapid head cooling on 96-hr neurologic outcomes and survival by heart rate variability analysis in a pig model of prolonged cardiac arrest. design:: prospective randomized controlled animal stu ... | 2011 | 21926589 |
| tracking cats: problems with placing feline carnivores on δo, δd isoscapes. | several felids are endangered and threatened by the illegal wildlife trade. establishing geographic origin of tissues of endangered species is thus crucial for wildlife crime investigations and effective conservation strategies. as shown in other species, stable isotope analysis of hydrogen and oxygen in hair (δd(h), δ(18)o(h)) can be used as a tool for provenance determination. however, reliably predicting the spatial distribution of δd(h) and δ(18)o(h) requires confirmation from animal tissues ... | 2011 | 21931770 |
| sensitive mie scattering immunoagglutination assay of porcine reproductive and respiratory syndrome virus (prrsv) from lung tissue samples in a microfluidic chip. | a microfluidic immunosensor utilizing mie scattering immunoaggultination assay was developed for rapid and sensitive detection of porcine reproductive and respiratory syndrome virus (prrsv) from lung tissue samples of domesticated pigs. antibodies against prrsv were conjugated to the surface of highly carboxylated polystyrene microparticles (diameter=920nm) and mixed with the diluted prrsv tissue samples in a y-shaped microchannel. antibody-antigen binding induced microparticle immunoagglutinati ... | 2011 | 21871925 |
| assessment of rodents as animal models for reston ebolavirus. | the emergence of reston ebolavirus (rebov) in domestic swine in the philippines has caused a renewed interest in rebov pathogenicity. here, the use of different rodent species as animal disease models for rebov was investigated. balb/c and stat1(-)(/-) mice, hartley guinea pigs, and syrian hamsters were inoculated intraperitoneally with rebov strain pennsylvania or reston08-a. although virus replication occurred in guinea pigs, hamsters, and stat1(-/-) mice, progression to disease was only obser ... | 2011 | 21987777 |
| evolution of the porcine (sus scrofa domestica) immunoglobulin kappa locus through germline gene conversion. | immunoglobulin (ig) gene rearrangement and expression are central to disease resistance and health maintenance in animals. the ig kappa (igk) locus in swine (sus scrofa domestica) contributes to approximately half of all antibody molecules, in contrast to many other cetartiodactyla, whose members provide the majority of human dietary protein and in which kappa locus utilization is limited. the porcine igk variable locus is 27.9 kb upstream of five ig kappa j genes (igkj) which are separated from ... | 2011 | 22109540 |